AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1329930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329930 MtrunA17_Chr4g0061171 100.000 160 0 0 1 160 1 160 2.83e-118 330
Msa1329930 MtrunA17_Chr3g0091481 96.250 160 6 0 1 160 1 160 5.53e-113 317
Msa1329930 MtrunA17_Chr4g0074021 93.711 159 10 0 1 159 1 159 2.73e-109 308
Msa1329930 MtrunA17_Chr5g0418211 91.824 159 13 0 1 159 1 159 9.03e-108 304
Msa1329930 MtrunA17_Chr4g0062241 71.569 102 19 1 1 102 1 92 4.95e-48 150
Msa1329930 MtrunA17_Chr4g0074011 46.094 128 21 2 18 145 42 121 1.23e-25 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329930 AT1G13950.1 91.195 159 13 1 1 159 1 158 7.23e-104 294
Msa1329930 AT1G69410.1 86.792 159 20 1 1 159 1 158 5.64e-101 287
Msa1329930 AT1G26630.1 81.646 158 28 1 1 158 1 157 3.47e-95 272
Msa1329930 AT1G26630.2 83.465 127 20 1 1 127 1 126 4.40e-76 223

Find 70 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAATGAACGCTAAAATTT+TGG 0.101895 8_4:-48873090 None:intergenic
AGTGGTGTTGGTGGTGTTTA+TGG 0.113621 8_4:+48874904 Msa1329930:three_prime_UTR
TCAAACGAGTATTTCCTTAT+TGG 0.168992 8_4:+48874976 Msa1329930:three_prime_UTR
GTGAAGATAAGTGGTAAATT+AGG 0.184677 8_4:-48873153 None:intergenic
CAAACGAGTATTTCCTTATT+GGG 0.252830 8_4:+48874977 Msa1329930:three_prime_UTR
TTCGTCAGACATATTGATTC+AGG 0.274394 8_4:-48873242 None:intergenic
ACTCATTTCATTCCATAGTT+TGG 0.285322 8_4:+48875185 Msa1329930:three_prime_UTR
CATCGGCCTTAGATTCAAAT+TGG 0.297056 8_4:-48873270 None:intergenic
CAATTTCCCCAACCAAACTA+TGG 0.307891 8_4:-48875197 None:intergenic
TGAAGATAAGTGGTAAATTA+GGG 0.331085 8_4:-48873152 None:intergenic
GTGAGCTTGCTCACTGAAAA+TGG 0.342511 8_4:+48874502 Msa1329930:CDS
TAAAGGACAAATACCCAATA+AGG 0.344877 8_4:-48874990 None:intergenic
CTTATTGACATTTCTGAAGA+TGG 0.348595 8_4:+48873766 Msa1329930:CDS
CTTGGCATGTCCATGCTTCC+CGG 0.359341 8_4:-48873509 None:intergenic
ATCTGTGCTTTGAAGGATAT+TGG 0.363464 8_4:+48874771 Msa1329930:CDS
TTTCATTCCATAGTTTGGTT+GGG 0.375187 8_4:+48875190 Msa1329930:three_prime_UTR
AGAGCACCAATTTGAATCTA+AGG 0.377994 8_4:+48873264 Msa1329930:CDS
CGATAATGAGAACAGAGATT+TGG 0.380861 8_4:-48873195 None:intergenic
AGCTTGTTGAGGATAAGTTT+TGG 0.388597 8_4:-48873302 None:intergenic
TACTATACTTGTAAAGATAA+AGG 0.392946 8_4:-48875007 None:intergenic
CTGTTCTCATTATCGCTCTT+CGG 0.419231 8_4:+48873203 Msa1329930:five_prime_UTR
CGTAAAACTCTGCTCTAGTC+TGG 0.419964 8_4:-48875080 None:intergenic
AATGTCAATAAGCTGATAAT+CGG 0.422180 8_4:-48873755 None:intergenic
ATTTCATTCCATAGTTTGGT+TGG 0.427612 8_4:+48875189 Msa1329930:three_prime_UTR
TTATAATATTCGCAGACCTC+AGG 0.458657 8_4:-48873115 None:intergenic
TTCATTCCATAGTTTGGTTG+GGG 0.466367 8_4:+48875191 Msa1329930:three_prime_UTR
GGAGCAGATCTGTGCTTTGA+AGG 0.467522 8_4:+48874764 Msa1329930:CDS
ATGAGAACAGAGATTTGGTT+CGG 0.473998 8_4:-48873190 None:intergenic
TAACTATGTATCCATTCTTA+CGG 0.477130 8_4:-48873333 None:intergenic
GCTGGTACCATCCGTAAGAA+TGG 0.484451 8_4:+48873322 Msa1329930:CDS
TGATTCAGGAGTGGCGGTGG+CGG 0.509670 8_4:-48873228 None:intergenic
TGTATCCGTGATGTCTGCTA+TGG 0.510337 8_4:+48874737 Msa1329930:CDS
CTTTGTGTGCAGATCAAGGA+TGG 0.512763 8_4:+48874690 Msa1329930:intron
CACTGAAAATGGAAACACTA+AGG 0.519818 8_4:+48874513 Msa1329930:CDS
TGTGAGACTAGTGGTGTTGG+TGG 0.526439 8_4:+48874895 Msa1329930:three_prime_UTR
GAAGTTTCGACTTCGAAGAC+CGG 0.527896 8_4:+48873490 Msa1329930:CDS
GAATAATCACCTTGCATGGA+CGG 0.531922 8_4:-48873363 None:intergenic
GGATGGATTTGCTGAGGGAA+AGG 0.534900 8_4:+48874707 Msa1329930:CDS
TGTCATCGGTGGGAAGTTTG+AGG 0.539905 8_4:-48874540 None:intergenic
CAATTGAAAGTGAAGATAAG+TGG 0.541516 8_4:-48873162 None:intergenic
ACTTCGAAGACCGGGAAGCA+TGG 0.555740 8_4:+48873499 Msa1329930:CDS
CTGAGTGAGCAGACTGTCAT+CGG 0.555770 8_4:-48874554 None:intergenic
TATTGATTCAGGAGTGGCGG+TGG 0.557198 8_4:-48873231 None:intergenic
TTTGAATCTAAGGCCGATGC+CGG 0.559985 8_4:+48873274 Msa1329930:CDS
TTACGAATAATCACCTTGCA+TGG 0.560163 8_4:-48873367 None:intergenic
TCTTGTGAGACTAGTGGTGT+TGG 0.565302 8_4:+48874892 Msa1329930:three_prime_UTR
AAAACTTATCCTCAACAAGC+TGG 0.581192 8_4:+48873304 Msa1329930:CDS
TAGCGTTCATTCCACGCCTG+AGG 0.590265 8_4:+48873099 Msa1329930:five_prime_UTR
AGTGAGCAGACTGTCATCGG+TGG 0.592770 8_4:-48874551 None:intergenic
AAGTTTCGACTTCGAAGACC+GGG 0.594274 8_4:+48873491 Msa1329930:CDS
TGTGCTTTGAAGGATATTGG+TGG 0.594489 8_4:+48874774 Msa1329930:CDS
CGGATGGTACCAGCTTGTTG+AGG 0.595998 8_4:-48873313 None:intergenic
CGTGATGTCTGCTATGGGTG+AGG 0.597852 8_4:+48874743 Msa1329930:CDS
CGATGACAGTCTGCTCACTC+AGG 0.597987 8_4:+48874555 Msa1329930:CDS
ATAGATTCTTGTGAGACTAG+TGG 0.603554 8_4:+48874886 Msa1329930:three_prime_UTR
TATGTATCCATTCTTACGGA+TGG 0.607030 8_4:-48873329 None:intergenic
ATTCACTTCAAAGAAGCTTG+AGG 0.607930 8_4:+48873555 Msa1329930:CDS
ACATCACAATTATGTGAAGA+CGG 0.610965 8_4:-48873586 None:intergenic
GTATCCGTGATGTCTGCTAT+GGG 0.612684 8_4:+48874738 Msa1329930:CDS
GTGAGCAGACTGTCATCGGT+GGG 0.615967 8_4:-48874550 None:intergenic
GATCAAGGATGGATTTGCTG+AGG 0.618132 8_4:+48874701 Msa1329930:intron
ATCAAGGATGGATTTGCTGA+GGG 0.621184 8_4:+48874702 Msa1329930:CDS
TTCATGCAATACATAAACTG+TGG 0.627363 8_4:-48875230 None:intergenic
AATAGCAACAAAATGACACT+TGG 0.628478 8_4:-48873527 None:intergenic
CAGACATCACGGATACAACA+AGG 0.636222 8_4:-48874731 None:intergenic
ACATATTGATTCAGGAGTGG+CGG 0.644758 8_4:-48873234 None:intergenic
CTCACCCATAGCAGACATCA+CGG 0.653023 8_4:-48874742 None:intergenic
ATATTCGCAGACCTCAGGCG+TGG 0.676343 8_4:-48873110 None:intergenic
CAGACATATTGATTCAGGAG+TGG 0.678905 8_4:-48873237 None:intergenic
TAATCGGTACGATTCACGTG+AGG 0.748646 8_4:-48873739 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAACAAACTAAATTAAAA+CGG + chr8_4:48874335-48874354 Msa1329930:intron 10.0%
!! TTAAACAAACTAAATTAAAA+CGG + chr8_4:48874335-48874354 Msa1329930:intron 10.0%
!!! CAATTGTTTTTATTTTTGTT+AGG + chr8_4:48873714-48873733 Msa1329930:intron 15.0%
!! TAAACAAACTAAATTAAAAC+GGG + chr8_4:48874336-48874355 Msa1329930:intron 15.0%
!!! CAATTGTTTTTATTTTTGTT+AGG + chr8_4:48873714-48873733 Msa1329930:intron 15.0%
!! TAAACAAACTAAATTAAAAC+GGG + chr8_4:48874336-48874355 Msa1329930:intron 15.0%
!! CACTTAATTAATCAAATCAA+AGG - chr8_4:48873820-48873839 None:intergenic 20.0%
!!! GATTTTGATATTCAATTAGT+TGG + chr8_4:48873897-48873916 Msa1329930:intron 20.0%
!!! GATTTTTTTACTTTCAGATT+AGG + chr8_4:48873955-48873974 Msa1329930:intron 20.0%
!!! AGCTTAGAAAAAAAGATTTT+GGG - chr8_4:48874297-48874316 None:intergenic 20.0%
!!! TTTAGTTTGTTTAAGCTTTA+TGG - chr8_4:48874329-48874348 None:intergenic 20.0%
!! TTCATAATATAGCTATCAAA+AGG - chr8_4:48874424-48874443 None:intergenic 20.0%
!!! TGATTATGTTTTTGTATTGT+AGG + chr8_4:48874480-48874499 Msa1329930:intron 20.0%
!! TACTATACTTGTAAAGATAA+AGG - chr8_4:48875010-48875029 None:intergenic 20.0%
!! CACTTAATTAATCAAATCAA+AGG - chr8_4:48873820-48873839 None:intergenic 20.0%
!!! GATTTTGATATTCAATTAGT+TGG + chr8_4:48873897-48873916 Msa1329930:intron 20.0%
!!! GATTTTTTTACTTTCAGATT+AGG + chr8_4:48873955-48873974 Msa1329930:intron 20.0%
!!! AGCTTAGAAAAAAAGATTTT+GGG - chr8_4:48874297-48874316 None:intergenic 20.0%
!!! TTTAGTTTGTTTAAGCTTTA+TGG - chr8_4:48874329-48874348 None:intergenic 20.0%
!! TTCATAATATAGCTATCAAA+AGG - chr8_4:48874424-48874443 None:intergenic 20.0%
!!! TGATTATGTTTTTGTATTGT+AGG + chr8_4:48874480-48874499 Msa1329930:intron 20.0%
!! TACTATACTTGTAAAGATAA+AGG - chr8_4:48875010-48875029 None:intergenic 20.0%
! TGAAGATAAGTGGTAAATTA+GGG - chr8_4:48873155-48873174 None:intergenic 25.0%
! TAACTATGTATCCATTCTTA+CGG - chr8_4:48873336-48873355 None:intergenic 25.0%
!! TTTTTGTACTTCGTTATTCA+TGG + chr8_4:48873418-48873437 Msa1329930:intron 25.0%
! AATGTCAATAAGCTGATAAT+CGG - chr8_4:48873758-48873777 None:intergenic 25.0%
! GTTCATACTTCTTTATAATC+TGG - chr8_4:48873844-48873863 None:intergenic 25.0%
! AAGTATGAACTGATTGTTAT+GGG + chr8_4:48873854-48873873 Msa1329930:intron 25.0%
!! TTAGTTGGAAATTTGTTTGA+AGG + chr8_4:48873912-48873931 Msa1329930:intron 25.0%
! TAATCTGATTTAGATTAGTG+TGG - chr8_4:48874238-48874257 None:intergenic 25.0%
!! GAGCTTAGAAAAAAAGATTT+TGG - chr8_4:48874298-48874317 None:intergenic 25.0%
! TGAAGATAAGTGGTAAATTA+GGG - chr8_4:48873155-48873174 None:intergenic 25.0%
! TAACTATGTATCCATTCTTA+CGG - chr8_4:48873336-48873355 None:intergenic 25.0%
!! TTTTTGTACTTCGTTATTCA+TGG + chr8_4:48873418-48873437 Msa1329930:intron 25.0%
! AATGTCAATAAGCTGATAAT+CGG - chr8_4:48873758-48873777 None:intergenic 25.0%
! GTTCATACTTCTTTATAATC+TGG - chr8_4:48873844-48873863 None:intergenic 25.0%
! AAGTATGAACTGATTGTTAT+GGG + chr8_4:48873854-48873873 Msa1329930:intron 25.0%
!! TTAGTTGGAAATTTGTTTGA+AGG + chr8_4:48873912-48873931 Msa1329930:intron 25.0%
! TAATCTGATTTAGATTAGTG+TGG - chr8_4:48874238-48874257 None:intergenic 25.0%
!! GAGCTTAGAAAAAAAGATTT+TGG - chr8_4:48874298-48874317 None:intergenic 25.0%
GTGAAGATAAGTGGTAAATT+AGG - chr8_4:48873156-48873175 None:intergenic 30.0%
CAATTGAAAGTGAAGATAAG+TGG - chr8_4:48873165-48873184 None:intergenic 30.0%
!!! TGTTTTTTGTTGTTGCGATT+AGG + chr8_4:48873462-48873481 Msa1329930:intron 30.0%
AATAGCAACAAAATGACACT+TGG - chr8_4:48873530-48873549 None:intergenic 30.0%
ACATCACAATTATGTGAAGA+CGG - chr8_4:48873589-48873608 None:intergenic 30.0%
! CTTATTGACATTTCTGAAGA+TGG + chr8_4:48873766-48873785 Msa1329930:CDS 30.0%
GAAGTATGAACTGATTGTTA+TGG + chr8_4:48873853-48873872 Msa1329930:intron 30.0%
! GGTACAATAACTTTTGTAAC+AGG - chr8_4:48874373-48874392 None:intergenic 30.0%
TAAGCCTGTTACAGATTAAT+GGG - chr8_4:48874395-48874414 None:intergenic 30.0%
ATAAGCCTGTTACAGATTAA+TGG - chr8_4:48874396-48874415 None:intergenic 30.0%
! GATTTGTTCTTGCATTGTAA+TGG + chr8_4:48874586-48874605 Msa1329930:intron 30.0%
! TGGTGTTTATGGATTTTTGT+TGG + chr8_4:48874915-48874934 Msa1329930:three_prime_UTR 30.0%
TCAAACGAGTATTTCCTTAT+TGG + chr8_4:48874976-48874995 Msa1329930:three_prime_UTR 30.0%
CAAACGAGTATTTCCTTATT+GGG + chr8_4:48874977-48874996 Msa1329930:three_prime_UTR 30.0%
TAAAGGACAAATACCCAATA+AGG - chr8_4:48874993-48875012 None:intergenic 30.0%
ACTCATTTCATTCCATAGTT+TGG + chr8_4:48875185-48875204 Msa1329930:three_prime_UTR 30.0%
ATTTCATTCCATAGTTTGGT+TGG + chr8_4:48875189-48875208 Msa1329930:three_prime_UTR 30.0%
TTTCATTCCATAGTTTGGTT+GGG + chr8_4:48875190-48875209 Msa1329930:three_prime_UTR 30.0%
TTCATGCAATACATAAACTG+TGG - chr8_4:48875233-48875252 None:intergenic 30.0%
! GGAATGAACGCTAAAATTTT+TGG - chr8_4:48873092-48873111 None:intergenic 30.0%
GTGAAGATAAGTGGTAAATT+AGG - chr8_4:48873156-48873175 None:intergenic 30.0%
CAATTGAAAGTGAAGATAAG+TGG - chr8_4:48873165-48873184 None:intergenic 30.0%
!!! TGTTTTTTGTTGTTGCGATT+AGG + chr8_4:48873462-48873481 Msa1329930:intron 30.0%
AATAGCAACAAAATGACACT+TGG - chr8_4:48873530-48873549 None:intergenic 30.0%
ACATCACAATTATGTGAAGA+CGG - chr8_4:48873589-48873608 None:intergenic 30.0%
! CTTATTGACATTTCTGAAGA+TGG + chr8_4:48873766-48873785 Msa1329930:CDS 30.0%
GAAGTATGAACTGATTGTTA+TGG + chr8_4:48873853-48873872 Msa1329930:intron 30.0%
! GGTACAATAACTTTTGTAAC+AGG - chr8_4:48874373-48874392 None:intergenic 30.0%
TAAGCCTGTTACAGATTAAT+GGG - chr8_4:48874395-48874414 None:intergenic 30.0%
ATAAGCCTGTTACAGATTAA+TGG - chr8_4:48874396-48874415 None:intergenic 30.0%
! GATTTGTTCTTGCATTGTAA+TGG + chr8_4:48874586-48874605 Msa1329930:intron 30.0%
! TGGTGTTTATGGATTTTTGT+TGG + chr8_4:48874915-48874934 Msa1329930:three_prime_UTR 30.0%
TCAAACGAGTATTTCCTTAT+TGG + chr8_4:48874976-48874995 Msa1329930:three_prime_UTR 30.0%
CAAACGAGTATTTCCTTATT+GGG + chr8_4:48874977-48874996 Msa1329930:three_prime_UTR 30.0%
TAAAGGACAAATACCCAATA+AGG - chr8_4:48874993-48875012 None:intergenic 30.0%
ACTCATTTCATTCCATAGTT+TGG + chr8_4:48875185-48875204 Msa1329930:three_prime_UTR 30.0%
ATTTCATTCCATAGTTTGGT+TGG + chr8_4:48875189-48875208 Msa1329930:three_prime_UTR 30.0%
TTTCATTCCATAGTTTGGTT+GGG + chr8_4:48875190-48875209 Msa1329930:three_prime_UTR 30.0%
TTCATGCAATACATAAACTG+TGG - chr8_4:48875233-48875252 None:intergenic 30.0%
TTATAATATTCGCAGACCTC+AGG - chr8_4:48873118-48873137 None:intergenic 35.0%
! ATGAGAACAGAGATTTGGTT+CGG - chr8_4:48873193-48873212 None:intergenic 35.0%
CGATAATGAGAACAGAGATT+TGG - chr8_4:48873198-48873217 None:intergenic 35.0%
!! TTCGTCAGACATATTGATTC+AGG - chr8_4:48873245-48873264 None:intergenic 35.0%
! AGAGCACCAATTTGAATCTA+AGG + chr8_4:48873264-48873283 Msa1329930:CDS 35.0%
!!! AGCTTGTTGAGGATAAGTTT+TGG - chr8_4:48873305-48873324 None:intergenic 35.0%
AAAACTTATCCTCAACAAGC+TGG + chr8_4:48873304-48873323 Msa1329930:CDS 35.0%
TATGTATCCATTCTTACGGA+TGG - chr8_4:48873332-48873351 None:intergenic 35.0%
TTACGAATAATCACCTTGCA+TGG - chr8_4:48873370-48873389 None:intergenic 35.0%
!!! TTTTTGTTGTTGCGATTAGG+TGG + chr8_4:48873465-48873484 Msa1329930:intron 35.0%
ATTCACTTCAAAGAAGCTTG+AGG + chr8_4:48873555-48873574 Msa1329930:CDS 35.0%
! GTTGGAAATTTGTTTGAAGG+AGG + chr8_4:48873915-48873934 Msa1329930:intron 35.0%
! TTCAGACCCTTTTATGTTGA+AGG + chr8_4:48874157-48874176 Msa1329930:intron 35.0%
! TCAGACCCTTTTATGTTGAA+GGG + chr8_4:48874158-48874177 Msa1329930:intron 35.0%
CCATACCCTTCAACATAAAA+GGG - chr8_4:48874166-48874185 None:intergenic 35.0%
CATACACAAAATCAACTCAC+CGG - chr8_4:48874189-48874208 None:intergenic 35.0%
AAGCCTGTTACAGATTAATG+GGG - chr8_4:48874394-48874413 None:intergenic 35.0%
CACTGAAAATGGAAACACTA+AGG + chr8_4:48874513-48874532 Msa1329930:CDS 35.0%
!! ATCTGTGCTTTGAAGGATAT+TGG + chr8_4:48874771-48874790 Msa1329930:CDS 35.0%
ATAGATTCTTGTGAGACTAG+TGG + chr8_4:48874886-48874905 Msa1329930:three_prime_UTR 35.0%
TTCATTCCATAGTTTGGTTG+GGG + chr8_4:48875191-48875210 Msa1329930:three_prime_UTR 35.0%
TTATAATATTCGCAGACCTC+AGG - chr8_4:48873118-48873137 None:intergenic 35.0%
! ATGAGAACAGAGATTTGGTT+CGG - chr8_4:48873193-48873212 None:intergenic 35.0%
CGATAATGAGAACAGAGATT+TGG - chr8_4:48873198-48873217 None:intergenic 35.0%
!! TTCGTCAGACATATTGATTC+AGG - chr8_4:48873245-48873264 None:intergenic 35.0%
! AGAGCACCAATTTGAATCTA+AGG + chr8_4:48873264-48873283 Msa1329930:CDS 35.0%
!!! AGCTTGTTGAGGATAAGTTT+TGG - chr8_4:48873305-48873324 None:intergenic 35.0%
AAAACTTATCCTCAACAAGC+TGG + chr8_4:48873304-48873323 Msa1329930:CDS 35.0%
TATGTATCCATTCTTACGGA+TGG - chr8_4:48873332-48873351 None:intergenic 35.0%
TTACGAATAATCACCTTGCA+TGG - chr8_4:48873370-48873389 None:intergenic 35.0%
!!! TTTTTGTTGTTGCGATTAGG+TGG + chr8_4:48873465-48873484 Msa1329930:intron 35.0%
ATTCACTTCAAAGAAGCTTG+AGG + chr8_4:48873555-48873574 Msa1329930:CDS 35.0%
! GTTGGAAATTTGTTTGAAGG+AGG + chr8_4:48873915-48873934 Msa1329930:intron 35.0%
! TTCAGACCCTTTTATGTTGA+AGG + chr8_4:48874157-48874176 Msa1329930:intron 35.0%
! TCAGACCCTTTTATGTTGAA+GGG + chr8_4:48874158-48874177 Msa1329930:intron 35.0%
CCATACCCTTCAACATAAAA+GGG - chr8_4:48874166-48874185 None:intergenic 35.0%
CATACACAAAATCAACTCAC+CGG - chr8_4:48874189-48874208 None:intergenic 35.0%
AAGCCTGTTACAGATTAATG+GGG - chr8_4:48874394-48874413 None:intergenic 35.0%
CACTGAAAATGGAAACACTA+AGG + chr8_4:48874513-48874532 Msa1329930:CDS 35.0%
!! ATCTGTGCTTTGAAGGATAT+TGG + chr8_4:48874771-48874790 Msa1329930:CDS 35.0%
ATAGATTCTTGTGAGACTAG+TGG + chr8_4:48874886-48874905 Msa1329930:three_prime_UTR 35.0%
TTCATTCCATAGTTTGGTTG+GGG + chr8_4:48875191-48875210 Msa1329930:three_prime_UTR 35.0%
CTGTTCTCATTATCGCTCTT+CGG + chr8_4:48873203-48873222 Msa1329930:five_prime_UTR 40.0%
!! ACATATTGATTCAGGAGTGG+CGG - chr8_4:48873237-48873256 None:intergenic 40.0%
!! CAGACATATTGATTCAGGAG+TGG - chr8_4:48873240-48873259 None:intergenic 40.0%
CATCGGCCTTAGATTCAAAT+TGG - chr8_4:48873273-48873292 None:intergenic 40.0%
!!! AGGATAAGTTTTGGAAGCAC+CGG - chr8_4:48873296-48873315 None:intergenic 40.0%
TATCAAAAACCGTCCATGCA+AGG + chr8_4:48873354-48873373 Msa1329930:CDS 40.0%
GAATAATCACCTTGCATGGA+CGG - chr8_4:48873366-48873385 None:intergenic 40.0%
! CCCTTTTATGTTGAAGGGTA+TGG + chr8_4:48874163-48874182 Msa1329930:intron 40.0%
GCCATACCCTTCAACATAAA+AGG - chr8_4:48874167-48874186 None:intergenic 40.0%
! TTTATGTTGAAGGGTATGGC+CGG + chr8_4:48874167-48874186 Msa1329930:intron 40.0%
GTACCCCATTAATCTGTAAC+AGG + chr8_4:48874388-48874407 Msa1329930:intron 40.0%
TTTGCTTTGTGTGCAGATCA+AGG + chr8_4:48874686-48874705 Msa1329930:intron 40.0%
!! ATCAAGGATGGATTTGCTGA+GGG + chr8_4:48874702-48874721 Msa1329930:CDS 40.0%
!! TGTGCTTTGAAGGATATTGG+TGG + chr8_4:48874774-48874793 Msa1329930:CDS 40.0%
CAATTTCCCCAACCAAACTA+TGG - chr8_4:48875200-48875219 None:intergenic 40.0%
CTGTTCTCATTATCGCTCTT+CGG + chr8_4:48873203-48873222 Msa1329930:five_prime_UTR 40.0%
!! ACATATTGATTCAGGAGTGG+CGG - chr8_4:48873237-48873256 None:intergenic 40.0%
!! CAGACATATTGATTCAGGAG+TGG - chr8_4:48873240-48873259 None:intergenic 40.0%
CATCGGCCTTAGATTCAAAT+TGG - chr8_4:48873273-48873292 None:intergenic 40.0%
!!! AGGATAAGTTTTGGAAGCAC+CGG - chr8_4:48873296-48873315 None:intergenic 40.0%
TATCAAAAACCGTCCATGCA+AGG + chr8_4:48873354-48873373 Msa1329930:CDS 40.0%
GAATAATCACCTTGCATGGA+CGG - chr8_4:48873366-48873385 None:intergenic 40.0%
! CCCTTTTATGTTGAAGGGTA+TGG + chr8_4:48874163-48874182 Msa1329930:intron 40.0%
GCCATACCCTTCAACATAAA+AGG - chr8_4:48874167-48874186 None:intergenic 40.0%
! TTTATGTTGAAGGGTATGGC+CGG + chr8_4:48874167-48874186 Msa1329930:intron 40.0%
GTACCCCATTAATCTGTAAC+AGG + chr8_4:48874388-48874407 Msa1329930:intron 40.0%
TTTGCTTTGTGTGCAGATCA+AGG + chr8_4:48874686-48874705 Msa1329930:intron 40.0%
!! ATCAAGGATGGATTTGCTGA+GGG + chr8_4:48874702-48874721 Msa1329930:CDS 40.0%
!! TGTGCTTTGAAGGATATTGG+TGG + chr8_4:48874774-48874793 Msa1329930:CDS 40.0%
CAATTTCCCCAACCAAACTA+TGG - chr8_4:48875200-48875219 None:intergenic 40.0%
! TTTGAATCTAAGGCCGATGC+CGG + chr8_4:48873274-48873293 Msa1329930:CDS 45.0%
GAAGTTTCGACTTCGAAGAC+CGG + chr8_4:48873490-48873509 Msa1329930:CDS 45.0%
AAGTTTCGACTTCGAAGACC+GGG + chr8_4:48873491-48873510 Msa1329930:CDS 45.0%
TAATCGGTACGATTCACGTG+AGG - chr8_4:48873742-48873761 None:intergenic 45.0%
! GTGAGCTTGCTCACTGAAAA+TGG + chr8_4:48874502-48874521 Msa1329930:CDS 45.0%
CTTTGTGTGCAGATCAAGGA+TGG + chr8_4:48874690-48874709 Msa1329930:intron 45.0%
!! GATCAAGGATGGATTTGCTG+AGG + chr8_4:48874701-48874720 Msa1329930:intron 45.0%
CAGACATCACGGATACAACA+AGG - chr8_4:48874734-48874753 None:intergenic 45.0%
TGTATCCGTGATGTCTGCTA+TGG + chr8_4:48874737-48874756 Msa1329930:CDS 45.0%
GTATCCGTGATGTCTGCTAT+GGG + chr8_4:48874738-48874757 Msa1329930:CDS 45.0%
TCTTGTGAGACTAGTGGTGT+TGG + chr8_4:48874892-48874911 Msa1329930:three_prime_UTR 45.0%
!! AGTGGTGTTGGTGGTGTTTA+TGG + chr8_4:48874904-48874923 Msa1329930:three_prime_UTR 45.0%
CGTAAAACTCTGCTCTAGTC+TGG - chr8_4:48875083-48875102 None:intergenic 45.0%
! TTTGAATCTAAGGCCGATGC+CGG + chr8_4:48873274-48873293 Msa1329930:CDS 45.0%
GAAGTTTCGACTTCGAAGAC+CGG + chr8_4:48873490-48873509 Msa1329930:CDS 45.0%
AAGTTTCGACTTCGAAGACC+GGG + chr8_4:48873491-48873510 Msa1329930:CDS 45.0%
TAATCGGTACGATTCACGTG+AGG - chr8_4:48873742-48873761 None:intergenic 45.0%
! GTGAGCTTGCTCACTGAAAA+TGG + chr8_4:48874502-48874521 Msa1329930:CDS 45.0%
CTTTGTGTGCAGATCAAGGA+TGG + chr8_4:48874690-48874709 Msa1329930:intron 45.0%
!! GATCAAGGATGGATTTGCTG+AGG + chr8_4:48874701-48874720 Msa1329930:intron 45.0%
CAGACATCACGGATACAACA+AGG - chr8_4:48874734-48874753 None:intergenic 45.0%
TGTATCCGTGATGTCTGCTA+TGG + chr8_4:48874737-48874756 Msa1329930:CDS 45.0%
GTATCCGTGATGTCTGCTAT+GGG + chr8_4:48874738-48874757 Msa1329930:CDS 45.0%
TCTTGTGAGACTAGTGGTGT+TGG + chr8_4:48874892-48874911 Msa1329930:three_prime_UTR 45.0%
!! AGTGGTGTTGGTGGTGTTTA+TGG + chr8_4:48874904-48874923 Msa1329930:three_prime_UTR 45.0%
CGTAAAACTCTGCTCTAGTC+TGG - chr8_4:48875083-48875102 None:intergenic 45.0%
!! TATTGATTCAGGAGTGGCGG+TGG - chr8_4:48873234-48873253 None:intergenic 50.0%
!!! AGTTTTGGAAGCACCGGCAT+CGG - chr8_4:48873290-48873309 None:intergenic 50.0%
GCTGGTACCATCCGTAAGAA+TGG + chr8_4:48873322-48873341 Msa1329930:CDS 50.0%
TGTCATCGGTGGGAAGTTTG+AGG - chr8_4:48874543-48874562 None:intergenic 50.0%
CTGAGTGAGCAGACTGTCAT+CGG - chr8_4:48874557-48874576 None:intergenic 50.0%
!! GGATGGATTTGCTGAGGGAA+AGG + chr8_4:48874707-48874726 Msa1329930:CDS 50.0%
CTCACCCATAGCAGACATCA+CGG - chr8_4:48874745-48874764 None:intergenic 50.0%
GGAGCAGATCTGTGCTTTGA+AGG + chr8_4:48874764-48874783 Msa1329930:CDS 50.0%
! TGTGAGACTAGTGGTGTTGG+TGG + chr8_4:48874895-48874914 Msa1329930:three_prime_UTR 50.0%
!! TATTGATTCAGGAGTGGCGG+TGG - chr8_4:48873234-48873253 None:intergenic 50.0%
!!! AGTTTTGGAAGCACCGGCAT+CGG - chr8_4:48873290-48873309 None:intergenic 50.0%
GCTGGTACCATCCGTAAGAA+TGG + chr8_4:48873322-48873341 Msa1329930:CDS 50.0%
TGTCATCGGTGGGAAGTTTG+AGG - chr8_4:48874543-48874562 None:intergenic 50.0%
CTGAGTGAGCAGACTGTCAT+CGG - chr8_4:48874557-48874576 None:intergenic 50.0%
!! GGATGGATTTGCTGAGGGAA+AGG + chr8_4:48874707-48874726 Msa1329930:CDS 50.0%
CTCACCCATAGCAGACATCA+CGG - chr8_4:48874745-48874764 None:intergenic 50.0%
GGAGCAGATCTGTGCTTTGA+AGG + chr8_4:48874764-48874783 Msa1329930:CDS 50.0%
! TGTGAGACTAGTGGTGTTGG+TGG + chr8_4:48874895-48874914 Msa1329930:three_prime_UTR 50.0%
TAGCGTTCATTCCACGCCTG+AGG + chr8_4:48873099-48873118 Msa1329930:five_prime_UTR 55.0%
ATATTCGCAGACCTCAGGCG+TGG - chr8_4:48873113-48873132 None:intergenic 55.0%
!! CGGATGGTACCAGCTTGTTG+AGG - chr8_4:48873316-48873335 None:intergenic 55.0%
ACTTCGAAGACCGGGAAGCA+TGG + chr8_4:48873499-48873518 Msa1329930:CDS 55.0%
CTTGGCATGTCCATGCTTCC+CGG - chr8_4:48873512-48873531 None:intergenic 55.0%
GTGAGCAGACTGTCATCGGT+GGG - chr8_4:48874553-48874572 None:intergenic 55.0%
AGTGAGCAGACTGTCATCGG+TGG - chr8_4:48874554-48874573 None:intergenic 55.0%
CGATGACAGTCTGCTCACTC+AGG + chr8_4:48874555-48874574 Msa1329930:CDS 55.0%
CGTGATGTCTGCTATGGGTG+AGG + chr8_4:48874743-48874762 Msa1329930:CDS 55.0%
TAGCGTTCATTCCACGCCTG+AGG + chr8_4:48873099-48873118 Msa1329930:five_prime_UTR 55.0%
ATATTCGCAGACCTCAGGCG+TGG - chr8_4:48873113-48873132 None:intergenic 55.0%
!! CGGATGGTACCAGCTTGTTG+AGG - chr8_4:48873316-48873335 None:intergenic 55.0%
ACTTCGAAGACCGGGAAGCA+TGG + chr8_4:48873499-48873518 Msa1329930:CDS 55.0%
CTTGGCATGTCCATGCTTCC+CGG - chr8_4:48873512-48873531 None:intergenic 55.0%
GTGAGCAGACTGTCATCGGT+GGG - chr8_4:48874553-48874572 None:intergenic 55.0%
AGTGAGCAGACTGTCATCGG+TGG - chr8_4:48874554-48874573 None:intergenic 55.0%
CGATGACAGTCTGCTCACTC+AGG + chr8_4:48874555-48874574 Msa1329930:CDS 55.0%
CGTGATGTCTGCTATGGGTG+AGG + chr8_4:48874743-48874762 Msa1329930:CDS 55.0%
TGATTCAGGAGTGGCGGTGG+CGG - chr8_4:48873231-48873250 None:intergenic 60.0%
TGATTCAGGAGTGGCGGTGG+CGG - chr8_4:48873231-48873250 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 48873092 48875269 48873092 ID=Msa1329930;Name=Msa1329930
chr8_4 mRNA 48873092 48875269 48873092 ID=Msa1329930-mRNA-1;Parent=Msa1329930;Name=Msa1329930-mRNA-1;_AED=0.12;_eAED=0.12;_QI=161|1|1|1|1|1|5|463|160
chr8_4 exon 48873092 48873375 48873092 ID=Msa1329930-mRNA-1:exon:14051;Parent=Msa1329930-mRNA-1
chr8_4 exon 48873484 48873606 48873484 ID=Msa1329930-mRNA-1:exon:14052;Parent=Msa1329930-mRNA-1
chr8_4 exon 48873736 48873792 48873736 ID=Msa1329930-mRNA-1:exon:14053;Parent=Msa1329930-mRNA-1
chr8_4 exon 48874502 48874576 48874502 ID=Msa1329930-mRNA-1:exon:14054;Parent=Msa1329930-mRNA-1
chr8_4 exon 48874702 48875269 48874702 ID=Msa1329930-mRNA-1:exon:14055;Parent=Msa1329930-mRNA-1
chr8_4 five_prime_UTR 48873092 48873252 48873092 ID=Msa1329930-mRNA-1:five_prime_utr;Parent=Msa1329930-mRNA-1
chr8_4 CDS 48873253 48873375 48873253 ID=Msa1329930-mRNA-1:cds;Parent=Msa1329930-mRNA-1
chr8_4 CDS 48873484 48873606 48873484 ID=Msa1329930-mRNA-1:cds;Parent=Msa1329930-mRNA-1
chr8_4 CDS 48873736 48873792 48873736 ID=Msa1329930-mRNA-1:cds;Parent=Msa1329930-mRNA-1
chr8_4 CDS 48874502 48874576 48874502 ID=Msa1329930-mRNA-1:cds;Parent=Msa1329930-mRNA-1
chr8_4 CDS 48874702 48874806 48874702 ID=Msa1329930-mRNA-1:cds;Parent=Msa1329930-mRNA-1
chr8_4 three_prime_UTR 48874807 48875269 48874807 ID=Msa1329930-mRNA-1:three_prime_utr;Parent=Msa1329930-mRNA-1
Gene Sequence

>Msa1329930

ATGTCTGACGAAGAGCACCAATTTGAATCTAAGGCCGATGCCGGTGCTTCCAAAACTTATCCTCAACAAGCTGGTACCATCCGTAAGAATGGATACATAGTTATCAAAAACCGTCCATGCAAGGTGGTTGAAGTTTCGACTTCGAAGACCGGGAAGCATGGACATGCCAAGTGTCATTTTGTTGCTATTGATATATTCACTTCAAAGAAGCTTGAGGATATCGTGCCGTCTTCACATAATTGTGATGTTCCTCACGTGAATCGTACCGATTATCAGCTTATTGACATTTCTGAAGATGGATTTGTGAGCTTGCTCACTGAAAATGGAAACACTAAGGATGACCTCAAACTTCCCACCGATGACAGTCTGCTCACTCAGATCAAGGATGGATTTGCTGAGGGAAAGGACCTTGTTGTATCCGTGATGTCTGCTATGGGTGAGGAGCAGATCTGTGCTTTGAAGGATATTGGTGGAAAGAACTAA
Protein sequence

>Msa1329930

MSDEEHQFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTDDSLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGGKN*