AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0799160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0799160 MtrunA17_Chr5g0404371 96.396 444 14 1 1 444 1 442 0.0 867
Msa0799160 MtrunA17_Chr6g0457381 28.926 363 208 9 86 420 7 347 1.34e-27 113
Msa0799160 MtrunA17_Chr6g0457381 37.589 141 85 2 64 203 196 334 6.78e-20 91.3
Msa0799160 MtrunA17_Chr6g0457381 25.907 386 160 13 28 337 57 392 2.40e-17 83.6
Msa0799160 MtrunA17_Chr6g0457381 23.701 308 185 6 137 442 4 263 1.23e-14 75.5
Msa0799160 MtrunA17_Chr3g0130621 26.634 413 218 17 26 423 34 376 2.07e-24 105
Msa0799160 MtrunA17_Chr3g0130621 23.232 396 235 9 56 442 8 343 4.33e-22 98.2
Msa0799160 MtrunA17_Chr3g0130621 27.302 315 158 10 141 444 39 293 7.38e-20 91.7
Msa0799160 MtrunA17_Chr3g0130621 25.212 353 192 12 28 372 89 377 7.67e-18 85.5
Msa0799160 MtrunA17_Chr3g0130621 30.350 257 154 9 28 273 141 383 4.87e-17 82.8
Msa0799160 MtrunA17_Chr1g0202151 26.585 410 221 14 55 442 270 621 3.03e-22 100
Msa0799160 MtrunA17_Chr8g0384631 24.233 326 177 8 108 423 41 306 2.44e-21 95.1
Msa0799160 MtrunA17_Chr8g0384631 35.079 191 113 5 26 215 69 249 1.46e-19 90.1
Msa0799160 MtrunA17_Chr8g0384631 23.404 329 183 11 53 372 39 307 7.42e-17 82.0
Msa0799160 MtrunA17_Chr8g0384631 23.607 305 173 7 143 442 24 273 1.64e-16 80.9
Msa0799160 MtrunA17_Chr8g0384631 27.778 198 117 5 256 443 41 222 1.85e-11 65.5
Msa0799160 MtrunA17_Chr3g0123301 24.866 374 251 11 70 416 153 523 9.11e-21 94.7
Msa0799160 MtrunA17_Chr3g0094741 24.682 393 216 13 64 434 320 654 2.85e-20 94.0
Msa0799160 MtrunA17_Chr7g0244411 26.303 422 223 16 24 424 233 587 3.76e-20 93.6
Msa0799160 MtrunA17_Chr7g0253611 24.714 437 238 14 21 443 148 507 4.23e-20 93.6
Msa0799160 MtrunA17_Chr4g0013271 25.428 409 219 14 38 424 45 389 2.01e-19 91.3
Msa0799160 MtrunA17_Chr4g0013271 25.382 327 219 11 136 443 35 355 3.12e-14 75.1
Msa0799160 MtrunA17_Chr6g0463421 26.250 400 207 15 55 428 267 604 2.90e-19 90.9
Msa0799160 MtrunA17_Chr7g0233601 24.878 410 239 11 83 441 47 438 2.11e-18 87.0
Msa0799160 MtrunA17_Chr7g0233601 35.185 162 102 2 55 216 252 410 4.56e-17 83.2
Msa0799160 MtrunA17_Chr7g0233601 26.260 377 204 10 31 392 124 441 3.67e-12 68.2
Msa0799160 MtrunA17_Chr7g0220131 27.425 299 146 9 52 328 162 411 1.21e-17 85.1
Msa0799160 MtrunA17_Chr7g0220131 26.648 364 212 17 66 390 65 412 1.29e-13 72.8
Msa0799160 MtrunA17_Chr7g0220131 29.798 198 113 7 246 435 41 220 2.39e-11 65.5
Msa0799160 MtrunA17_Chr5g0415871 26.494 385 218 14 84 433 16 370 1.52e-17 84.0
Msa0799160 MtrunA17_Chr2g0317861 27.302 315 171 7 135 442 128 391 1.61e-17 84.7
Msa0799160 MtrunA17_Chr1g0167471 26.136 352 226 11 123 443 219 567 2.02e-17 85.1
Msa0799160 MtrunA17_Chr1g0167471 23.636 385 238 14 55 403 265 629 2.16e-15 79.0
Msa0799160 MtrunA17_Chr8g0362061 25.000 404 218 18 64 442 286 629 8.68e-17 83.2
Msa0799160 MtrunA17_Chr5g0442001 23.469 392 221 13 64 434 310 643 1.26e-16 82.8
Msa0799160 MtrunA17_Chr7g0239601 35.583 163 87 7 28 185 58 207 3.11e-16 77.4
Msa0799160 MtrunA17_Chr7g0239601 31.217 189 112 5 259 441 31 207 1.60e-13 69.7
Msa0799160 MtrunA17_Chr3g0130871 25.697 323 174 9 135 438 47 322 2.59e-15 76.6
Msa0799160 MtrunA17_Chr3g0130871 26.531 294 142 9 169 444 28 265 3.79e-13 70.1
Msa0799160 MtrunA17_Chr3g0130871 25.566 309 176 8 51 354 17 276 8.93e-12 66.2
Msa0799160 MtrunA17_Chr3g0109811 22.704 392 211 12 64 428 305 631 6.63e-15 77.4
Msa0799160 MtrunA17_Chr3g0109811 24.373 279 171 6 158 407 292 559 1.80e-14 75.9
Msa0799160 MtrunA17_Chr3g0112381 38.971 136 72 5 55 185 29 158 5.86e-14 69.7
Msa0799160 MtrunA17_Chr3g0140201 23.729 354 184 12 99 417 28 330 9.55e-14 73.2
Msa0799160 MtrunA17_Chr3g0142381 24.735 283 158 5 159 429 45 284 3.72e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0799160 AT5G48330.1 60.230 435 152 7 19 443 31 454 6.24e-178 505
Msa0799160 AT5G48330.2 60.138 434 153 7 19 443 31 453 1.56e-177 504
Msa0799160 AT3G02510.1 28.375 363 209 9 86 420 9 348 2.48e-28 115
Msa0799160 AT3G02510.1 34.507 142 90 2 63 203 196 335 1.82e-17 84.3
Msa0799160 AT5G63860.1 26.914 405 224 14 28 423 36 377 1.10e-27 114
Msa0799160 AT5G63860.1 27.362 307 199 8 28 321 89 384 1.05e-21 97.4
Msa0799160 AT5G63860.1 22.423 388 245 8 58 442 10 344 3.00e-21 96.3
Msa0799160 AT5G63860.1 25.843 356 194 11 95 443 1 293 1.63e-18 87.8
Msa0799160 AT5G16040.1 27.548 363 212 9 86 420 9 348 5.30e-27 112
Msa0799160 AT5G16040.1 27.431 288 135 7 65 330 152 387 6.62e-19 88.6
Msa0799160 AT5G16040.1 33.099 142 92 2 63 203 196 335 7.07e-18 85.5
Msa0799160 AT5G16040.1 25.733 307 179 5 137 442 6 264 2.74e-16 80.9
Msa0799160 AT3G02510.2 27.729 339 170 8 86 411 9 285 2.83e-22 97.4
Msa0799160 AT3G02510.2 25.405 370 193 11 26 379 5 307 6.94e-16 78.6
Msa0799160 AT3G02510.2 24.921 317 167 9 137 441 6 263 1.72e-11 65.5
Msa0799160 AT3G02510.3 27.762 353 176 10 84 420 1 290 3.04e-22 97.8
Msa0799160 AT3G02510.3 34.507 142 90 2 63 203 138 277 1.08e-17 84.3
Msa0799160 AT3G02510.3 26.621 293 141 9 65 335 94 334 3.15e-17 82.8
Msa0799160 AT3G02510.3 26.250 320 161 8 138 442 1 260 7.87e-17 81.6
Msa0799160 AT5G12350.1 24.615 390 218 12 64 434 309 641 1.52e-20 95.5
Msa0799160 AT5G12350.1 24.810 395 207 17 29 401 330 656 2.66e-12 69.7
Msa0799160 AT5G19420.3 24.041 391 219 12 64 434 313 645 4.28e-20 94.0
Msa0799160 AT5G19420.3 26.230 244 144 9 65 281 419 653 7.75e-11 65.1
Msa0799160 AT1G76950.1 25.240 416 221 16 55 442 272 625 4.57e-20 94.0
Msa0799160 AT5G19420.2 24.745 392 215 14 64 434 347 679 5.15e-20 93.6
Msa0799160 AT5G19420.1 24.745 392 215 14 64 434 313 645 5.28e-20 93.6
Msa0799160 AT3G26100.5 25.197 381 241 9 70 416 150 520 1.36e-19 91.7
Msa0799160 AT3G26100.6 25.197 381 241 9 70 416 150 520 1.36e-19 91.7
Msa0799160 AT3G26100.2 25.197 381 241 9 70 416 150 520 1.36e-19 91.7
Msa0799160 AT3G26100.3 25.197 381 241 9 70 416 50 420 1.41e-19 90.9
Msa0799160 AT3G26100.4 25.197 381 241 9 70 416 50 420 1.41e-19 90.9
Msa0799160 AT3G26100.1 25.197 381 241 9 70 416 50 420 1.41e-19 90.9
Msa0799160 AT1G65920.2 23.664 393 213 10 64 433 247 575 5.04e-19 90.5
Msa0799160 AT1G65920.2 24.615 325 221 10 138 443 215 534 2.04e-12 70.1
Msa0799160 AT1G65920.1 23.664 393 213 10 64 433 293 621 5.43e-19 90.5
Msa0799160 AT1G65920.1 24.615 325 221 10 138 443 261 580 2.63e-12 69.7
Msa0799160 AT3G55580.1 26.379 417 248 13 49 441 102 483 5.75e-19 89.7
Msa0799160 AT3G55580.1 31.217 189 109 7 6 185 307 483 1.12e-11 67.0
Msa0799160 AT5G60870.1 24.485 437 245 14 59 443 39 442 6.71e-19 89.0
Msa0799160 AT5G60870.1 26.857 350 174 9 65 392 154 443 1.71e-17 84.7
Msa0799160 AT5G60870.1 30.864 162 109 3 55 216 254 412 9.59e-17 82.4
Msa0799160 AT5G60870.1 32.857 140 85 3 53 185 303 440 9.81e-14 73.2
Msa0799160 AT3G53830.3 26.540 422 243 15 46 441 87 467 1.10e-18 88.6
Msa0799160 AT3G53830.4 29.431 299 163 10 158 441 230 495 4.80e-18 86.7
Msa0799160 AT5G08710.1 26.444 450 251 16 19 442 36 431 5.64e-18 86.3
Msa0799160 AT5G42140.2 25.189 397 227 14 55 434 268 611 8.63e-18 86.7
Msa0799160 AT5G42140.1 25.189 397 227 14 55 434 268 611 8.63e-18 86.7
Msa0799160 AT3G03790.1 26.173 405 213 13 17 405 141 475 9.70e-18 86.7
Msa0799160 AT5G60870.2 26.801 347 185 9 65 392 154 450 1.18e-17 85.5
Msa0799160 AT5G60870.2 30.178 169 108 4 55 216 254 419 9.41e-15 76.6
Msa0799160 AT1G27060.1 26.410 390 229 10 28 417 17 348 1.22e-17 84.7
Msa0799160 AT5G60870.3 30.864 162 109 3 55 216 85 243 2.49e-17 82.4
Msa0799160 AT5G60870.3 32.857 140 85 3 53 185 134 271 4.61e-14 72.8
Msa0799160 AT3G03790.3 26.271 354 185 10 65 405 188 478 3.17e-17 85.1
Msa0799160 AT3G03790.3 28.082 292 156 10 16 296 192 440 6.86e-14 74.7
Msa0799160 AT3G03790.2 26.271 354 185 10 65 405 188 478 3.60e-17 84.7
Msa0799160 AT3G03790.2 28.082 292 156 10 16 296 192 440 7.59e-14 74.3
Msa0799160 AT3G23270.1 24.408 422 228 18 48 441 235 593 4.03e-17 84.7
Msa0799160 AT3G23270.1 26.178 382 215 14 80 443 209 541 5.70e-17 84.3
Msa0799160 AT3G23270.2 24.408 422 228 18 48 441 239 597 4.91e-17 84.3
Msa0799160 AT3G23270.2 26.178 382 215 14 80 443 213 545 6.83e-17 84.0
Msa0799160 AT4G14368.1 25.316 395 208 17 55 424 261 593 5.28e-17 84.3
Msa0799160 AT4G14368.1 25.392 319 187 10 132 443 285 559 8.24e-17 84.0
Msa0799160 AT4G14368.3 25.472 318 186 10 132 442 278 551 9.77e-17 83.6
Msa0799160 AT4G14368.3 25.381 394 207 17 55 423 254 585 1.43e-16 83.2
Msa0799160 AT4G14368.2 25.472 318 186 10 132 442 283 556 1.08e-16 83.6
Msa0799160 AT4G14368.2 25.381 394 207 17 55 423 259 590 1.42e-16 83.2
Msa0799160 AT4G14368.4 25.472 318 186 10 132 442 285 558 1.13e-16 83.2
Msa0799160 AT4G14368.4 25.381 394 207 17 55 423 261 592 1.54e-16 82.8
Msa0799160 AT3G02300.2 27.243 301 149 11 65 336 179 438 1.88e-16 82.0
Msa0799160 AT3G02300.2 24.242 363 222 11 66 390 75 422 1.43e-13 72.8
Msa0799160 AT3G02300.2 35.172 145 80 4 268 407 75 210 4.35e-12 68.2
Msa0799160 AT3G02300.1 27.243 301 149 11 65 336 168 427 2.02e-16 81.6
Msa0799160 AT3G02300.1 24.242 363 222 11 66 390 64 411 1.80e-13 72.4
Msa0799160 AT3G02300.1 30.851 188 107 5 268 441 64 242 4.24e-12 68.2
Msa0799160 AT5G11580.1 25.513 439 222 15 28 413 37 423 3.06e-16 81.3
Msa0799160 AT5G11580.1 27.300 337 183 13 135 416 34 363 9.11e-15 77.0
Msa0799160 AT5G16040.2 25.817 306 178 5 137 441 6 263 3.27e-16 80.1
Msa0799160 AT5G16040.2 25.469 373 193 12 26 381 5 309 1.23e-15 78.2
Msa0799160 AT3G15430.6 24.697 413 243 9 43 444 38 393 3.91e-16 80.9
Msa0799160 AT3G15430.4 24.697 413 243 9 43 444 38 393 3.91e-16 80.9
Msa0799160 AT3G15430.2 24.697 413 243 9 43 444 38 393 3.91e-16 80.9
Msa0799160 AT3G15430.3 24.697 413 243 9 43 444 38 393 3.91e-16 80.9
Msa0799160 AT3G15430.5 24.697 413 243 9 43 444 38 393 3.91e-16 80.9
Msa0799160 AT3G15430.1 24.697 413 243 9 43 444 38 393 3.91e-16 80.9
Msa0799160 AT5G60870.4 27.953 254 131 5 65 312 154 361 7.97e-16 79.3
Msa0799160 AT5G60870.4 31.818 132 87 3 55 186 254 382 2.66e-12 68.6
Msa0799160 AT1G19880.1 26.710 307 152 8 159 444 45 299 7.41e-15 77.0
Msa0799160 AT3G47660.7 28.374 289 177 12 46 312 186 466 1.54e-14 76.6
Msa0799160 AT3G47660.7 24.598 435 240 19 30 442 119 487 2.47e-14 75.9
Msa0799160 AT3G47660.6 28.374 289 177 12 46 312 253 533 1.56e-14 76.6
Msa0799160 AT3G47660.6 24.598 435 240 19 30 442 186 554 2.39e-14 75.9
Msa0799160 AT3G47660.4 27.742 310 190 13 46 329 367 668 2.99e-14 75.9
Msa0799160 AT3G47660.4 24.368 435 241 18 30 442 300 668 4.08e-14 75.5
Msa0799160 AT3G47660.3 24.598 435 240 19 30 442 261 629 3.08e-14 75.5
Msa0799160 AT3G47660.5 24.598 435 240 19 30 442 261 629 3.08e-14 75.5
Msa0799160 AT3G47660.2 24.598 435 240 19 30 442 264 632 3.25e-14 75.5
Msa0799160 AT3G47660.1 24.598 435 240 19 30 442 264 632 3.43e-14 75.5
Msa0799160 AT5G08710.2 27.066 351 190 13 19 357 36 332 5.96e-13 70.1
Msa0799160 AT5G08710.2 25.959 339 173 13 87 407 54 332 6.84e-13 70.1
Msa0799160 AT1G69710.2 25.914 301 191 9 136 407 247 544 7.28e-13 71.2
Msa0799160 AT1G69710.1 25.914 301 191 9 136 407 260 557 7.51e-13 71.2

Find 178 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGGGTCTGTTTGGGTTT+GGG 0.143939 5_3:+12250604 Msa0799160:CDS
GACACATCTTTGAACTTAAA+AGG 0.155222 5_3:-12252690 None:intergenic
TCTAACCCCTCCACTCTTCT+TGG 0.209669 5_3:-12250524 None:intergenic
CAAAATTTGAAAACCTTCTA+AGG 0.230502 5_3:-12252514 None:intergenic
CCACTTCACAAGGCGATCTT+TGG 0.235793 5_3:+12250435 Msa0799160:CDS
AGATGGAAGGATAGGGAATA+TGG 0.260984 5_3:+12251591 Msa0799160:CDS
ATGATGGGTCTGTTTGGGTT+TGG 0.261109 5_3:+12250603 Msa0799160:CDS
AGACTAACATGCCAATAATA+TGG 0.268553 5_3:+12251737 Msa0799160:CDS
GCCTTGATTGATGCGTTGGC+TGG 0.288036 5_3:+12252345 Msa0799160:CDS
CACAAGGCGATCTTTGGGCT+TGG 0.291957 5_3:+12250441 Msa0799160:CDS
GGGGCGAGCTAGAGAAGATT+TGG 0.300080 5_3:+12252140 Msa0799160:CDS
CTGTGGATGGCAAGCTGTTT+GGG 0.306555 5_3:+12251554 Msa0799160:CDS
ACAAGGCGATCTTTGGGCTT+GGG 0.310684 5_3:+12250442 Msa0799160:CDS
AAGTCCAAGCTGTCCCCTTT+TGG 0.320606 5_3:-12250633 None:intergenic
AGGCGAGGTTCCCATATTAT+TGG 0.321168 5_3:-12251748 None:intergenic
CAACAACATTTATTTACAAT+TGG 0.321991 5_3:-12252771 None:intergenic
CATAATCTGGTTCTTGTTGC+TGG 0.330642 5_3:+12252558 Msa0799160:CDS
GATGGTGCCCTCTTGAGTTC+TGG 0.335543 5_3:+12252096 Msa0799160:CDS
GAAACAGGTCTCGTTTGGTT+GGG 0.339280 5_3:+12251513 Msa0799160:intron
CCAACAACCACCGTTGGTAT+AGG 0.342749 5_3:+12250326 Msa0799160:CDS
AGCGGGGCTTGGCCACTCTT+TGG 0.346640 5_3:+12252206 Msa0799160:CDS
CACTTCACAAGGCGATCTTT+GGG 0.348125 5_3:+12250436 Msa0799160:CDS
TTGGTGATGATGGGTCTGTT+TGG 0.349821 5_3:+12250597 Msa0799160:CDS
GTCTGGGTTTGATCATAATC+TGG 0.355645 5_3:+12252545 Msa0799160:CDS
TCGTGTTGTGTTGGTGATGA+TGG 0.355668 5_3:+12250587 Msa0799160:CDS
TGGTGATGATGGGTCTGTTT+GGG 0.357075 5_3:+12250598 Msa0799160:CDS
TCAGATAGTAATGTCACTTT+GGG 0.357204 5_3:+12252249 Msa0799160:CDS
AGCCCCGCTGCTACGGAAAC+AGG 0.364716 5_3:-12252192 None:intergenic
TTCAGCAGGGCGTGCACATT+CGG 0.368046 5_3:+12252386 Msa0799160:CDS
GAAATGTGGGTCTGGGGATC+TGG 0.369104 5_3:+12252429 Msa0799160:CDS
GATGGGTCTGTTTGGGTTTG+GGG 0.371073 5_3:+12250605 Msa0799160:CDS
TCAGAAGGTAAAGAAGGAAT+TGG 0.376055 5_3:-12250371 None:intergenic
GGATGGCAAGCTGTTTGGGT+GGG 0.376413 5_3:+12251558 Msa0799160:CDS
TAGAAACAACGAAGCCCAAC+TGG 0.377481 5_3:+12250466 Msa0799160:CDS
ACTCCTGTTTCCGTAGCAGC+GGG 0.387778 5_3:+12252189 Msa0799160:CDS
GCACTTGAACTGGAATCTAA+TGG 0.394490 5_3:-12251637 None:intergenic
GTTTCCGTAGCAGCGGGGCT+TGG 0.395218 5_3:+12252195 Msa0799160:CDS
CTCTTTAAAGCTTAAACTAA+AGG 0.399840 5_3:+12252712 Msa0799160:three_prime_UTR
AGTAACGTGTATGGCCAATT+GGG 0.400946 5_3:+12252120 Msa0799160:CDS
CAAAAGGAGAAATGTGGGTC+TGG 0.402480 5_3:+12252421 Msa0799160:CDS
TGATTGAAACAGGTCTCGTT+TGG 0.413513 5_3:+12251508 Msa0799160:intron
ACTGTGGATGGCAAGCTGTT+TGG 0.414500 5_3:+12251553 Msa0799160:CDS
CGCCCTGCTGAAACTGAAAC+CGG 0.414741 5_3:-12252375 None:intergenic
TCTTTAAAGCTTAAACTAAA+GGG 0.414926 5_3:+12252713 Msa0799160:three_prime_UTR
GGTTCACTAAATTTAGTAAC+CGG 0.417729 5_3:+12252613 Msa0799160:three_prime_UTR
TCCTGCCTTGATTGATGCGT+TGG 0.421410 5_3:+12252341 Msa0799160:CDS
AATAATATGGGAACCTCGCC+TGG 0.424364 5_3:+12251750 Msa0799160:CDS
AAAAGGGGACAGCTTGGACT+TGG 0.426798 5_3:+12250635 Msa0799160:CDS
GTTTGAAAATGCACTTGAAC+TGG 0.426915 5_3:-12251647 None:intergenic
TGCGTTGGCTGGGGAAAATC+CGG 0.427799 5_3:+12252356 Msa0799160:CDS
CTTCTCTAGCTCGCCCCAAT+TGG 0.427816 5_3:-12252134 None:intergenic
AAGTAACGTGTATGGCCAAT+TGG 0.428223 5_3:+12252119 Msa0799160:CDS
GGGCGAGCTAGAGAAGATTT+GGG 0.429340 5_3:+12252141 Msa0799160:CDS
ATCAGATAGTAATGTCACTT+TGG 0.429388 5_3:+12252248 Msa0799160:CDS
CCTTGATTGATGCGTTGGCT+GGG 0.431283 5_3:+12252346 Msa0799160:CDS
AATTGGATCAAGGGAGTGCT+TGG 0.433676 5_3:-12252754 None:intergenic
GGAGTGAACTTTATGTCAAC+AGG 0.436438 5_3:-12252171 None:intergenic
GAAAGTGCTTCAACTCTAGA+AGG 0.437031 5_3:-12250683 None:intergenic
GATGGCAAGCTGTTTGGGTG+GGG 0.439388 5_3:+12251559 Msa0799160:CDS
AGTTCTCAGCTAGGACGGGC+AGG 0.440448 5_3:+12252306 Msa0799160:CDS
CGTGTTGTGTTGGTGATGAT+GGG 0.440936 5_3:+12250588 Msa0799160:CDS
GACTACAGATATTGCTTCAT+GGG 0.446195 5_3:+12252272 Msa0799160:CDS
ACACATCTTTGAACTTAAAA+GGG 0.447389 5_3:-12252689 None:intergenic
CATTTAATCATGAATAAAGA+TGG 0.448731 5_3:+12252842 Msa0799160:three_prime_UTR
AAACAGGTCTCGTTTGGTTG+GGG 0.450780 5_3:+12251514 Msa0799160:intron
TGAAACAGGTCTCGTTTGGT+TGG 0.450912 5_3:+12251512 Msa0799160:intron
TGGATGGCAAGCTGTTTGGG+TGG 0.459079 5_3:+12251557 Msa0799160:CDS
ATTTATTTACAATTGGATCA+AGG 0.459687 5_3:-12252764 None:intergenic
CAAGGCGATCTTTGGGCTTG+GGG 0.460820 5_3:+12250443 Msa0799160:CDS
ATCAATCAAGGCAGGAAGAT+AGG 0.464337 5_3:-12252334 None:intergenic
CCTATACCAACGGTGGTTGT+TGG 0.466016 5_3:-12250326 None:intergenic
AATCCGGTTTCAGTTTCAGC+AGG 0.467662 5_3:+12252372 Msa0799160:CDS
ATGAGCACGGTGATGAGTTT+CGG 0.468069 5_3:+12250275 Msa0799160:CDS
ATCATCTTTACTACTTATCA+AGG 0.468134 5_3:-12252816 None:intergenic
GGAAAGCCCACGACCCAGTT+GGG 0.471539 5_3:-12250480 None:intergenic
TTAGAAAATGTGAAGGTTTG+TGG 0.475248 5_3:+12250542 Msa0799160:CDS
AAAAGGAGAAATGTGGGTCT+GGG 0.480674 5_3:+12252422 Msa0799160:CDS
AACGAAGCCCAACTGGGTCG+TGG 0.486773 5_3:+12250473 Msa0799160:CDS
TCGGTTGCTCTCACATCAAA+AGG 0.487958 5_3:+12252405 Msa0799160:CDS
ATTTAATCATGAATAAAGAT+GGG 0.493498 5_3:+12252843 Msa0799160:three_prime_UTR
ATGGGTCTGTTTGGGTTTGG+GGG 0.497631 5_3:+12250606 Msa0799160:CDS
GAAAACCTTCTAAGGAATCC+AGG 0.497690 5_3:-12252506 None:intergenic
GGACTACAGATATTGCTTCA+TGG 0.498556 5_3:+12252271 Msa0799160:CDS
GACTAACATGCCAATAATAT+GGG 0.499069 5_3:+12251738 Msa0799160:CDS
CCCAGCCAACGCATCAATCA+AGG 0.500231 5_3:-12252346 None:intergenic
TATTATGCAGATGGAAGGAT+AGG 0.501045 5_3:+12251583 Msa0799160:CDS
GGCGATCACAGAGAATATTC+CGG 0.501138 5_3:+12250207 Msa0799160:CDS
GTGGCCAAGCCCCGCTGCTA+CGG 0.501725 5_3:-12252199 None:intergenic
TCAACAAATGCCTTAGTGCT+AGG 0.503940 5_3:+12252592 Msa0799160:three_prime_UTR
TCTAAGGAATCCAGGTAAAA+CGG 0.504606 5_3:-12252498 None:intergenic
TTTATTTACAATTGGATCAA+GGG 0.505190 5_3:-12252763 None:intergenic
ATCAAGGCAGGAAGATAGGC+AGG 0.508105 5_3:-12252330 None:intergenic
GGTAAAGAAGGAATTGGAGT+TGG 0.508424 5_3:-12250365 None:intergenic
TTCTCGACAAAACGTAGTTA+AGG 0.508526 5_3:+12250703 Msa0799160:CDS
AACATAGGAAAGAGTGTACA+AGG 0.509568 5_3:+12252654 Msa0799160:three_prime_UTR
CTTGATTGATGCGTTGGCTG+GGG 0.510648 5_3:+12252347 Msa0799160:CDS
GATGGAAGGATAGGGAATAT+GGG 0.512559 5_3:+12251592 Msa0799160:CDS
CGGAAAGCCCACGACCCAGT+TGG 0.514415 5_3:-12250481 None:intergenic
ACAATATCAGAAGGTAAAGA+AGG 0.516505 5_3:-12250377 None:intergenic
TCACCGTGCTCATCCATCTC+CGG 0.521429 5_3:-12250265 None:intergenic
CGGTTGTTATCCACCGGAGA+TGG 0.522709 5_3:+12250252 Msa0799160:CDS
AAAGAAAGTCTTACAAGGAA+TGG 0.524779 5_3:+12251708 Msa0799160:CDS
ATGTCCAAAAGGGGACAGCT+TGG 0.527513 5_3:+12250629 Msa0799160:CDS
CTCATTCCATGTTTCTCTAG+CGG 0.530612 5_3:-12250501 None:intergenic
AAAGAACTAAAGAGTGATCA+AGG 0.530943 5_3:-12251816 None:intergenic
TACTATCTGATTCATCAAGC+TGG 0.532238 5_3:-12252236 None:intergenic
GGTGTTGTTTCGTGTTGTGT+TGG 0.534055 5_3:+12250578 Msa0799160:CDS
CACTCCTGTTTCCGTAGCAG+CGG 0.534340 5_3:+12252188 Msa0799160:CDS
CCAAAGATCGCCTTGTGAAG+TGG 0.541795 5_3:-12250435 None:intergenic
AGTTCTGGAAGTAACGTGTA+TGG 0.542194 5_3:+12252111 Msa0799160:CDS
GACGGAACCCACGGAGCACT+CGG 0.542264 5_3:+12250299 Msa0799160:CDS
ACTGCGTGGCGTTCAAGTTG+TGG 0.548356 5_3:+12251780 Msa0799160:CDS
GGGTGGGGCTATTATGCAGA+TGG 0.551116 5_3:+12251574 Msa0799160:CDS
CACATCAAAAGGAGAAATGT+GGG 0.554457 5_3:+12252416 Msa0799160:CDS
CCCGTCCTAGCTGAGAACTC+TGG 0.559607 5_3:-12252302 None:intergenic
TGCGAGAGAGTGGTAGTGAC+CGG 0.560541 5_3:-12250411 None:intergenic
TGGTAAACCGAGTGCTCCGT+GGG 0.572538 5_3:-12250306 None:intergenic
GCTCTTGCATTAACTGTGGA+TGG 0.574863 5_3:+12251541 Msa0799160:CDS
ATTTAGTGAACCTAGCACTA+AGG 0.575101 5_3:-12252602 None:intergenic
GTTCTCAGCTAGGACGGGCA+GGG 0.575236 5_3:+12252307 Msa0799160:CDS
CGCAGTTCTTCAACCAGGCG+AGG 0.575726 5_3:-12251763 None:intergenic
ACCAGAGTTCTCAGCTAGGA+CGG 0.577035 5_3:+12252301 Msa0799160:CDS
CCAGAGTTCTCAGCTAGGAC+GGG 0.578114 5_3:+12252302 Msa0799160:CDS
ACGAAGCCCAACTGGGTCGT+GGG 0.579776 5_3:+12250474 Msa0799160:CDS
TCACATCAAAAGGAGAAATG+TGG 0.579944 5_3:+12252415 Msa0799160:CDS
GAATATGGGGAATGATCACT+TGG 0.580284 5_3:+12251606 Msa0799160:CDS
TTGGTAAACCGAGTGCTCCG+TGG 0.581895 5_3:-12250307 None:intergenic
GCCAACGCATCAATCAAGGC+AGG 0.582821 5_3:-12252342 None:intergenic
CCGCCGCGGTTGTTATCCAC+CGG 0.586164 5_3:+12250246 Msa0799160:CDS
AGTCTTACAAGGAATGGCAG+AGG 0.587231 5_3:+12251714 Msa0799160:CDS
GGTTTACCAACAACCACCGT+TGG 0.588291 5_3:+12250320 Msa0799160:CDS
TTAAAGCTTAAACTAAAGGG+TGG 0.590686 5_3:+12252716 Msa0799160:three_prime_UTR
CGCCACGCAGTTCTTCAACC+AGG 0.592349 5_3:-12251768 None:intergenic
AACAGGTCTCGTTTGGTTGG+GGG 0.594975 5_3:+12251515 Msa0799160:intron
GTGGTAGTGACCGGCGTGGA+CGG 0.596464 5_3:-12250402 None:intergenic
CGTTACTTCCAGAACTCAAG+AGG 0.596715 5_3:-12252104 None:intergenic
ATGGAAGGATAGGGAATATG+GGG 0.598459 5_3:+12251593 Msa0799160:CDS
AACAGATAAGAAAATAACAT+AGG 0.605445 5_3:+12252639 Msa0799160:three_prime_UTR
AGTTTCGGCGACGGAACCCA+CGG 0.608836 5_3:+12250290 Msa0799160:CDS
AGTAGAAAAGAAAAGAAATG+TGG 0.609485 5_3:+12250186 Msa0799160:exon
AAAGGAGAAATGTGGGTCTG+GGG 0.610343 5_3:+12252423 Msa0799160:CDS
GAATGAGCCAAGAAGAGTGG+AGG 0.612574 5_3:+12250517 Msa0799160:CDS
ATGGAATGAGCCAAGAAGAG+TGG 0.613681 5_3:+12250514 Msa0799160:CDS
GGCTTTCCGCTAGAGAAACA+TGG 0.616376 5_3:+12250495 Msa0799160:CDS
GATATTGTTACCGTCCACGC+CGG 0.620563 5_3:+12250392 Msa0799160:CDS
GTGAAGTGGTTGCGAGAGAG+TGG 0.623822 5_3:-12250421 None:intergenic
TTTAATCATGAATAAAGATG+GGG 0.624732 5_3:+12252844 Msa0799160:three_prime_UTR
AATGAGCCAAGAAGAGTGGA+GGG 0.624889 5_3:+12250518 Msa0799160:CDS
GCTGAAAAGAAAGTCTTACA+AGG 0.625036 5_3:+12251703 Msa0799160:CDS
CCGTGCTCATCCATCTCCGG+TGG 0.625588 5_3:-12250262 None:intergenic
TGCGTGTGTTCCTATACCAA+CGG 0.630934 5_3:-12250336 None:intergenic
GTAACGTGTATGGCCAATTG+GGG 0.635104 5_3:+12252121 Msa0799160:CDS
ATCCGGTTTCAGTTTCAGCA+GGG 0.636572 5_3:+12252373 Msa0799160:CDS
ATTATGCAGATGGAAGGATA+GGG 0.638426 5_3:+12251584 Msa0799160:CDS
GGAGGGGTTAGAAAATGTGA+AGG 0.639727 5_3:+12250535 Msa0799160:CDS
GCTGGCATATTGCCAAAGAG+TGG 0.644997 5_3:-12252218 None:intergenic
GGGGCTATTATGCAGATGGA+AGG 0.650368 5_3:+12251578 Msa0799160:CDS
GCACGCTCTTGCATTAACTG+TGG 0.652360 5_3:+12251537 Msa0799160:CDS
TCTCTCGCAACCACTTCACA+AGG 0.655415 5_3:+12250425 Msa0799160:CDS
ACTACAGATATTGCTTCATG+GGG 0.658687 5_3:+12252273 Msa0799160:CDS
AGAAACAACGAAGCCCAACT+GGG 0.659931 5_3:+12250467 Msa0799160:CDS
CAAGTTGTGGACATTGCATG+TGG 0.663017 5_3:+12251793 Msa0799160:CDS
TGGAACCAGAGTTCTCAGCT+AGG 0.674318 5_3:+12252297 Msa0799160:CDS
ACGGTGATGAGTTTCGGCGA+CGG 0.675231 5_3:+12250281 Msa0799160:CDS
TGGACGGTAACAATATCAGA+AGG 0.675890 5_3:-12250386 None:intergenic
ATTCAAGCATGACACCGCCA+CGG 0.676456 5_3:+12250160 None:intergenic
CTCCTGTTTCCGTAGCAGCG+GGG 0.681037 5_3:+12252190 Msa0799160:CDS
CAGATAGTAATGTCACTTTG+GGG 0.684131 5_3:+12252250 Msa0799160:CDS
CCACCGGAGATGGATGAGCA+CGG 0.687533 5_3:+12250262 Msa0799160:CDS
GTTACTTCCAGAACTCAAGA+GGG 0.707147 5_3:-12252103 None:intergenic
ACATAGGAAAGAGTGTACAA+GGG 0.723463 5_3:+12252655 Msa0799160:three_prime_UTR
ATGAGCCAAGAAGAGTGGAG+GGG 0.735998 5_3:+12250519 Msa0799160:CDS
GAGAGTGGTAGTGACCGGCG+TGG 0.738365 5_3:-12250406 None:intergenic
CTACAGATATTGCTTCATGG+GGG 0.740225 5_3:+12252274 Msa0799160:CDS
TCTCCGGTGGATAACAACCG+CGG 0.747489 5_3:-12250249 None:intergenic
CGCCTGGTTGAAGAACTGCG+TGG 0.750941 5_3:+12251766 Msa0799160:CDS
CCGGTGGATAACAACCGCGG+CGG 0.765384 5_3:-12250246 None:intergenic
GTGTGTTCCTATACCAACGG+TGG 0.765520 5_3:-12250333 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTTATAAAATACTATTT+TGG + chr5_3:12250890-12250909 Msa0799160:intron 10.0%
!!! TTAGTTCTTTTTCTAAATTT+AGG + chr5_3:12251066-12251085 Msa0799160:intron 15.0%
!!! TCAATTTTCTCAAATTATTA+CGG - chr5_3:12251360-12251379 None:intergenic 15.0%
!!! ATATGTAATAATTTGCTAAA+AGG - chr5_3:12252031-12252050 None:intergenic 15.0%
!! ATTTAATCATGAATAAAGAT+GGG + chr5_3:12252843-12252862 Msa0799160:three_prime_UTR 15.0%
!! TAAAAAGTAGCTTATACTAT+TGG - chr5_3:12250877-12250896 None:intergenic 20.0%
!!! AATGTAGATTATGTTACTTA+CGG - chr5_3:12251843-12251862 None:intergenic 20.0%
!!! TCTACATTGCATAATTTTTT+AGG + chr5_3:12251855-12251874 Msa0799160:intron 20.0%
!!! CTACATTGCATAATTTTTTA+GGG + chr5_3:12251856-12251875 Msa0799160:intron 20.0%
!!! TATTTTCTTATCTGTTATTC+CGG - chr5_3:12252635-12252654 None:intergenic 20.0%
!! AACAGATAAGAAAATAACAT+AGG + chr5_3:12252639-12252658 Msa0799160:three_prime_UTR 20.0%
!!! TCTTTAAAGCTTAAACTAAA+GGG + chr5_3:12252713-12252732 Msa0799160:three_prime_UTR 20.0%
!! TTTATTTACAATTGGATCAA+GGG - chr5_3:12252766-12252785 None:intergenic 20.0%
!! ATTTATTTACAATTGGATCA+AGG - chr5_3:12252767-12252786 None:intergenic 20.0%
!! CAACAACATTTATTTACAAT+TGG - chr5_3:12252774-12252793 None:intergenic 20.0%
!! CATTTAATCATGAATAAAGA+TGG + chr5_3:12252842-12252861 Msa0799160:three_prime_UTR 20.0%
!! TTTAATCATGAATAAAGATG+GGG + chr5_3:12252844-12252863 Msa0799160:three_prime_UTR 20.0%
! AGTAGAAAAGAAAAGAAATG+TGG + chr5_3:12250186-12250205 Msa0799160:exon 25.0%
! CCAAACTAATAATTTACTCT+TGG - chr5_3:12250958-12250977 None:intergenic 25.0%
! CCAAGAGTAAATTATTAGTT+TGG + chr5_3:12250955-12250974 Msa0799160:intron 25.0%
! TTTGGTCTATGAAATTGTAA+GGG + chr5_3:12250973-12250992 Msa0799160:intron 25.0%
!!! ATTTAGGATCACAATTTTGA+AGG + chr5_3:12251082-12251101 Msa0799160:intron 25.0%
! TCAATGTTTACGTGATTTAT+TGG + chr5_3:12251143-12251162 Msa0799160:intron 25.0%
! GATATAGAGACTAAATTGAA+TGG + chr5_3:12251197-12251216 Msa0799160:intron 25.0%
!! AATGGCCTTCTTTAATTTAA+GGG + chr5_3:12251215-12251234 Msa0799160:intron 25.0%
! TTAGTCCCTTAAATTAAAGA+AGG - chr5_3:12251223-12251242 None:intergenic 25.0%
! GTAAAAGAGCTTATTTGAAT+AGG - chr5_3:12251324-12251343 None:intergenic 25.0%
! TTAAATGTAATCTCATGTGT+CGG + chr5_3:12251385-12251404 Msa0799160:intron 25.0%
! CCATCTTTAATACAAAGTAT+CGG + chr5_3:12251429-12251448 Msa0799160:intron 25.0%
!! ATTGTTTCTTTGATTGAAAC+AGG + chr5_3:12251498-12251517 Msa0799160:intron 25.0%
!!! AATTTAGTCTTTGAAATTGC+AGG + chr5_3:12251886-12251905 Msa0799160:intron 25.0%
! CAAAATTTGAAAACCTTCTA+AGG - chr5_3:12252517-12252536 None:intergenic 25.0%
! ACACATCTTTGAACTTAAAA+GGG - chr5_3:12252692-12252711 None:intergenic 25.0%
!!! CTCTTTAAAGCTTAAACTAA+AGG + chr5_3:12252712-12252731 Msa0799160:three_prime_UTR 25.0%
! ATCATCTTTACTACTTATCA+AGG - chr5_3:12252819-12252838 None:intergenic 25.0%
ACAATATCAGAAGGTAAAGA+AGG - chr5_3:12250380-12250399 None:intergenic 30.0%
TTAGAAAATGTGAAGGTTTG+TGG + chr5_3:12250542-12250561 Msa0799160:CDS 30.0%
TTAAGCTCTCTTACATTACA+TGG + chr5_3:12250739-12250758 Msa0799160:intron 30.0%
ATAACTTATGCAATGCTTGT+AGG - chr5_3:12250778-12250797 None:intergenic 30.0%
CAATGTTGATATTGTTAGAC+TGG - chr5_3:12250839-12250858 None:intergenic 30.0%
GTTTGGTCTATGAAATTGTA+AGG + chr5_3:12250972-12250991 Msa0799160:intron 30.0%
!!! ACGTGATTTATTGGTTTTGA+TGG + chr5_3:12251152-12251171 Msa0799160:intron 30.0%
! GAATGGCCTTCTTTAATTTA+AGG + chr5_3:12251214-12251233 Msa0799160:intron 30.0%
!!! TTGCTATTTTGTGAATCCAA+AGG + chr5_3:12251248-12251267 Msa0799160:intron 30.0%
CCGATACTTTGTATTAAAGA+TGG - chr5_3:12251432-12251451 None:intergenic 30.0%
AAAGAAAGTCTTACAAGGAA+TGG + chr5_3:12251708-12251727 Msa0799160:CDS 30.0%
AGACTAACATGCCAATAATA+TGG + chr5_3:12251737-12251756 Msa0799160:CDS 30.0%
GACTAACATGCCAATAATAT+GGG + chr5_3:12251738-12251757 Msa0799160:CDS 30.0%
AAAGAACTAAAGAGTGATCA+AGG - chr5_3:12251819-12251838 None:intergenic 30.0%
TTTCCAATTTCCTTATGTTC+AGG + chr5_3:12251946-12251965 Msa0799160:intron 30.0%
TTCCAATTTCCTTATGTTCA+GGG + chr5_3:12251947-12251966 Msa0799160:intron 30.0%
! ATCAGATAGTAATGTCACTT+TGG + chr5_3:12252248-12252267 Msa0799160:CDS 30.0%
!! TCAGATAGTAATGTCACTTT+GGG + chr5_3:12252249-12252268 Msa0799160:CDS 30.0%
! AGAAGGTTTTCAAATTTTGC+AGG + chr5_3:12252518-12252537 Msa0799160:CDS 30.0%
GGTTCACTAAATTTAGTAAC+CGG + chr5_3:12252613-12252632 Msa0799160:three_prime_UTR 30.0%
GACACATCTTTGAACTTAAA+AGG - chr5_3:12252693-12252712 None:intergenic 30.0%
TTAAAGCTTAAACTAAAGGG+TGG + chr5_3:12252716-12252735 Msa0799160:three_prime_UTR 30.0%
!!! ATGTTGTTGTTACTTTTGTC+TGG + chr5_3:12252785-12252804 Msa0799160:three_prime_UTR 30.0%
! TTTTCTTTTCTACTTCACCG+TGG - chr5_3:12250180-12250199 None:intergenic 35.0%
TCAGAAGGTAAAGAAGGAAT+TGG - chr5_3:12250374-12250393 None:intergenic 35.0%
TTCTCGACAAAACGTAGTTA+AGG + chr5_3:12250703-12250722 Msa0799160:CDS 35.0%
GTCTATGAAATTGTAAGGGT+TGG + chr5_3:12250977-12250996 Msa0799160:intron 35.0%
GCATAGCTTATTCAAACACA+TGG + chr5_3:12251456-12251475 Msa0799160:intron 35.0%
TATTATGCAGATGGAAGGAT+AGG + chr5_3:12251583-12251602 Msa0799160:CDS 35.0%
ATTATGCAGATGGAAGGATA+GGG + chr5_3:12251584-12251603 Msa0799160:CDS 35.0%
GTTTGAAAATGCACTTGAAC+TGG - chr5_3:12251650-12251669 None:intergenic 35.0%
GCTGAAAAGAAAGTCTTACA+AGG + chr5_3:12251703-12251722 Msa0799160:CDS 35.0%
TACTATCTGATTCATCAAGC+TGG - chr5_3:12252239-12252258 None:intergenic 35.0%
!! CAGATAGTAATGTCACTTTG+GGG + chr5_3:12252250-12252269 Msa0799160:CDS 35.0%
GACTACAGATATTGCTTCAT+GGG + chr5_3:12252272-12252291 Msa0799160:CDS 35.0%
ACTACAGATATTGCTTCATG+GGG + chr5_3:12252273-12252292 Msa0799160:CDS 35.0%
TCACATCAAAAGGAGAAATG+TGG + chr5_3:12252415-12252434 Msa0799160:CDS 35.0%
CACATCAAAAGGAGAAATGT+GGG + chr5_3:12252416-12252435 Msa0799160:CDS 35.0%
TCTGGTAAAAATGGTAGACT+TGG + chr5_3:12252447-12252466 Msa0799160:CDS 35.0%
TCTAAGGAATCCAGGTAAAA+CGG - chr5_3:12252501-12252520 None:intergenic 35.0%
! TTTTACCTGGATTCCTTAGA+AGG + chr5_3:12252501-12252520 Msa0799160:CDS 35.0%
! AGGTTTTCAAATTTTGCAGG+CGG + chr5_3:12252521-12252540 Msa0799160:CDS 35.0%
ATTTAGTGAACCTAGCACTA+AGG - chr5_3:12252605-12252624 None:intergenic 35.0%
! AACATAGGAAAGAGTGTACA+AGG + chr5_3:12252654-12252673 Msa0799160:three_prime_UTR 35.0%
! ACATAGGAAAGAGTGTACAA+GGG + chr5_3:12252655-12252674 Msa0799160:three_prime_UTR 35.0%
!!! CGGTTTTTGGTTTGAAGTTC+CGG - chr5_3:12250229-12250248 None:intergenic 40.0%
GGTAAAGAAGGAATTGGAGT+TGG - chr5_3:12250368-12250387 None:intergenic 40.0%
TGGACGGTAACAATATCAGA+AGG - chr5_3:12250389-12250408 None:intergenic 40.0%
CTCATTCCATGTTTCTCTAG+CGG - chr5_3:12250504-12250523 None:intergenic 40.0%
!! GAAAGTGCTTCAACTCTAGA+AGG - chr5_3:12250686-12250705 None:intergenic 40.0%
CGTCGAGCAATTAAATCCTT+TGG - chr5_3:12251267-12251286 None:intergenic 40.0%
TGATTGAAACAGGTCTCGTT+TGG + chr5_3:12251508-12251527 Msa0799160:intron 40.0%
AGATGGAAGGATAGGGAATA+TGG + chr5_3:12251591-12251610 Msa0799160:CDS 40.0%
GATGGAAGGATAGGGAATAT+GGG + chr5_3:12251592-12251611 Msa0799160:CDS 40.0%
ATGGAAGGATAGGGAATATG+GGG + chr5_3:12251593-12251612 Msa0799160:CDS 40.0%
GAATATGGGGAATGATCACT+TGG + chr5_3:12251606-12251625 Msa0799160:CDS 40.0%
GCACTTGAACTGGAATCTAA+TGG - chr5_3:12251640-12251659 None:intergenic 40.0%
! CTTTCTTTTCAGCAACTTCG+AGG - chr5_3:12251696-12251715 None:intergenic 40.0%
GTCCCTGAACATAAGGAAAT+TGG - chr5_3:12251952-12251971 None:intergenic 40.0%
CAACTTTGTCCCTGAACATA+AGG - chr5_3:12251959-12251978 None:intergenic 40.0%
GTTACTTCCAGAACTCAAGA+GGG - chr5_3:12252106-12252125 None:intergenic 40.0%
!! AGTTCTGGAAGTAACGTGTA+TGG + chr5_3:12252111-12252130 Msa0799160:CDS 40.0%
AAGTAACGTGTATGGCCAAT+TGG + chr5_3:12252119-12252138 Msa0799160:CDS 40.0%
AGTAACGTGTATGGCCAATT+GGG + chr5_3:12252120-12252139 Msa0799160:CDS 40.0%
GGAGTGAACTTTATGTCAAC+AGG - chr5_3:12252174-12252193 None:intergenic 40.0%
GGACTACAGATATTGCTTCA+TGG + chr5_3:12252271-12252290 Msa0799160:CDS 40.0%
CTACAGATATTGCTTCATGG+GGG + chr5_3:12252274-12252293 Msa0799160:CDS 40.0%
ATCAATCAAGGCAGGAAGAT+AGG - chr5_3:12252337-12252356 None:intergenic 40.0%
AAAAGGAGAAATGTGGGTCT+GGG + chr5_3:12252422-12252441 Msa0799160:CDS 40.0%
! GAAAACCTTCTAAGGAATCC+AGG - chr5_3:12252509-12252528 None:intergenic 40.0%
GTCTGGGTTTGATCATAATC+TGG + chr5_3:12252545-12252564 Msa0799160:CDS 40.0%
!! CATAATCTGGTTCTTGTTGC+TGG + chr5_3:12252558-12252577 Msa0799160:CDS 40.0%
TCAACAAATGCCTTAGTGCT+AGG + chr5_3:12252592-12252611 Msa0799160:three_prime_UTR 40.0%
! TCTTTTCTACTTCACCGTGG+CGG - chr5_3:12250177-12250196 None:intergenic 45.0%
GGCGATCACAGAGAATATTC+CGG + chr5_3:12250207-12250226 Msa0799160:CDS 45.0%
ATGAGCACGGTGATGAGTTT+CGG + chr5_3:12250275-12250294 Msa0799160:CDS 45.0%
TGCGTGTGTTCCTATACCAA+CGG - chr5_3:12250339-12250358 None:intergenic 45.0%
CACTTCACAAGGCGATCTTT+GGG + chr5_3:12250436-12250455 Msa0799160:CDS 45.0%
TAGAAACAACGAAGCCCAAC+TGG + chr5_3:12250466-12250485 Msa0799160:CDS 45.0%
AGAAACAACGAAGCCCAACT+GGG + chr5_3:12250467-12250486 Msa0799160:CDS 45.0%
ATGGAATGAGCCAAGAAGAG+TGG + chr5_3:12250514-12250533 Msa0799160:CDS 45.0%
AATGAGCCAAGAAGAGTGGA+GGG + chr5_3:12250518-12250537 Msa0799160:CDS 45.0%
GGAGGGGTTAGAAAATGTGA+AGG + chr5_3:12250535-12250554 Msa0799160:CDS 45.0%
! GTTTGTGGTGCTTTTGCTTC+TGG + chr5_3:12250557-12250576 Msa0799160:CDS 45.0%
!! GGTGTTGTTTCGTGTTGTGT+TGG + chr5_3:12250578-12250597 Msa0799160:CDS 45.0%
!! TCGTGTTGTGTTGGTGATGA+TGG + chr5_3:12250587-12250606 Msa0799160:CDS 45.0%
!! CGTGTTGTGTTGGTGATGAT+GGG + chr5_3:12250588-12250607 Msa0799160:CDS 45.0%
!! TTGGTGATGATGGGTCTGTT+TGG + chr5_3:12250597-12250616 Msa0799160:CDS 45.0%
!! TGGTGATGATGGGTCTGTTT+GGG + chr5_3:12250598-12250617 Msa0799160:CDS 45.0%
!! ATGATGGGTCTGTTTGGGTT+TGG + chr5_3:12250603-12250622 Msa0799160:CDS 45.0%
! TGATGGGTCTGTTTGGGTTT+GGG + chr5_3:12250604-12250623 Msa0799160:CDS 45.0%
ATGAGATCCCATTTCGAACC+AGG + chr5_3:12250803-12250822 Msa0799160:intron 45.0%
TGAGATCCCATTTCGAACCA+GGG + chr5_3:12250804-12250823 Msa0799160:intron 45.0%
TGAAACAGGTCTCGTTTGGT+TGG + chr5_3:12251512-12251531 Msa0799160:intron 45.0%
GAAACAGGTCTCGTTTGGTT+GGG + chr5_3:12251513-12251532 Msa0799160:intron 45.0%
AAACAGGTCTCGTTTGGTTG+GGG + chr5_3:12251514-12251533 Msa0799160:intron 45.0%
GCTCTTGCATTAACTGTGGA+TGG + chr5_3:12251541-12251560 Msa0799160:CDS 45.0%
AGTCTTACAAGGAATGGCAG+AGG + chr5_3:12251714-12251733 Msa0799160:CDS 45.0%
AGGCGAGGTTCCCATATTAT+TGG - chr5_3:12251751-12251770 None:intergenic 45.0%
AATAATATGGGAACCTCGCC+TGG + chr5_3:12251750-12251769 Msa0799160:CDS 45.0%
CAAGTTGTGGACATTGCATG+TGG + chr5_3:12251793-12251812 Msa0799160:CDS 45.0%
CGTTACTTCCAGAACTCAAG+AGG - chr5_3:12252107-12252126 None:intergenic 45.0%
GTAACGTGTATGGCCAATTG+GGG + chr5_3:12252121-12252140 Msa0799160:CDS 45.0%
AATCCGGTTTCAGTTTCAGC+AGG + chr5_3:12252372-12252391 Msa0799160:CDS 45.0%
ATCCGGTTTCAGTTTCAGCA+GGG + chr5_3:12252373-12252392 Msa0799160:CDS 45.0%
TCGGTTGCTCTCACATCAAA+AGG + chr5_3:12252405-12252424 Msa0799160:CDS 45.0%
CAAAAGGAGAAATGTGGGTC+TGG + chr5_3:12252421-12252440 Msa0799160:CDS 45.0%
AAAGGAGAAATGTGGGTCTG+GGG + chr5_3:12252423-12252442 Msa0799160:CDS 45.0%
GTCTGGGGATCTGGTAAAAA+TGG + chr5_3:12252438-12252457 Msa0799160:CDS 45.0%
! AAATTTTGCAGGCGGTGTCT+GGG + chr5_3:12252529-12252548 Msa0799160:CDS 45.0%
!! AATTGGATCAAGGGAGTGCT+TGG - chr5_3:12252757-12252776 None:intergenic 45.0%
!!! TTTTTATAAAATACTATTTT+GGG + chr5_3:12250891-12250910 Msa0799160:intron 5.0%
CTTCAAACCAAAAACCGCCG+CGG + chr5_3:12250232-12250251 Msa0799160:CDS 50.0%
! ATAACAACCGCGGCGGTTTT+TGG - chr5_3:12250242-12250261 None:intergenic 50.0%
GGTTTACCAACAACCACCGT+TGG + chr5_3:12250320-12250339 Msa0799160:CDS 50.0%
CCTATACCAACGGTGGTTGT+TGG - chr5_3:12250329-12250348 None:intergenic 50.0%
CCAACAACCACCGTTGGTAT+AGG + chr5_3:12250326-12250345 Msa0799160:CDS 50.0%
GTGTGTTCCTATACCAACGG+TGG - chr5_3:12250336-12250355 None:intergenic 50.0%
GATATTGTTACCGTCCACGC+CGG + chr5_3:12250392-12250411 Msa0799160:CDS 50.0%
TCTCTCGCAACCACTTCACA+AGG + chr5_3:12250425-12250444 Msa0799160:CDS 50.0%
CCAAAGATCGCCTTGTGAAG+TGG - chr5_3:12250438-12250457 None:intergenic 50.0%
CCACTTCACAAGGCGATCTT+TGG + chr5_3:12250435-12250454 Msa0799160:CDS 50.0%
ACAAGGCGATCTTTGGGCTT+GGG + chr5_3:12250442-12250461 Msa0799160:CDS 50.0%
GGCTTTCCGCTAGAGAAACA+TGG + chr5_3:12250495-12250514 Msa0799160:CDS 50.0%
GAATGAGCCAAGAAGAGTGG+AGG + chr5_3:12250517-12250536 Msa0799160:CDS 50.0%
ATGAGCCAAGAAGAGTGGAG+GGG + chr5_3:12250519-12250538 Msa0799160:CDS 50.0%
TCTAACCCCTCCACTCTTCT+TGG - chr5_3:12250527-12250546 None:intergenic 50.0%
! GATGGGTCTGTTTGGGTTTG+GGG + chr5_3:12250605-12250624 Msa0799160:CDS 50.0%
! ATGGGTCTGTTTGGGTTTGG+GGG + chr5_3:12250606-12250625 Msa0799160:CDS 50.0%
GGTTTGGGGGATGTCCAAAA+GGG + chr5_3:12250619-12250638 Msa0799160:CDS 50.0%
GTTTGGGGGATGTCCAAAAG+GGG + chr5_3:12250620-12250639 Msa0799160:CDS 50.0%
ATGTCCAAAAGGGGACAGCT+TGG + chr5_3:12250629-12250648 Msa0799160:CDS 50.0%
! AAGTCCAAGCTGTCCCCTTT+TGG - chr5_3:12250636-12250655 None:intergenic 50.0%
AAAAGGGGACAGCTTGGACT+TGG + chr5_3:12250635-12250654 Msa0799160:CDS 50.0%
AACAGGTCTCGTTTGGTTGG+GGG + chr5_3:12251515-12251534 Msa0799160:intron 50.0%
GCACGCTCTTGCATTAACTG+TGG + chr5_3:12251537-12251556 Msa0799160:CDS 50.0%
ACTGTGGATGGCAAGCTGTT+TGG + chr5_3:12251553-12251572 Msa0799160:CDS 50.0%
CTGTGGATGGCAAGCTGTTT+GGG + chr5_3:12251554-12251573 Msa0799160:CDS 50.0%
GGGGCTATTATGCAGATGGA+AGG + chr5_3:12251578-12251597 Msa0799160:CDS 50.0%
AACTCTCGAGTGCCCTTATC+AGG + chr5_3:12252072-12252091 Msa0799160:intron 50.0%
! AGGGCACCATCACCTGATAA+GGG - chr5_3:12252087-12252106 None:intergenic 50.0%
GGGCGAGCTAGAGAAGATTT+GGG + chr5_3:12252141-12252160 Msa0799160:CDS 50.0%
GCTGGCATATTGCCAAAGAG+TGG - chr5_3:12252221-12252240 None:intergenic 50.0%
CAGATATTGCTTCATGGGGG+TGG + chr5_3:12252277-12252296 Msa0799160:CDS 50.0%
TGGAACCAGAGTTCTCAGCT+AGG + chr5_3:12252297-12252316 Msa0799160:CDS 50.0%
ACCAGAGTTCTCAGCTAGGA+CGG + chr5_3:12252301-12252320 Msa0799160:CDS 50.0%
ATCAAGGCAGGAAGATAGGC+AGG - chr5_3:12252333-12252352 None:intergenic 50.0%
TCCTGCCTTGATTGATGCGT+TGG + chr5_3:12252341-12252360 Msa0799160:CDS 50.0%
!! CCTTGATTGATGCGTTGGCT+GGG + chr5_3:12252346-12252365 Msa0799160:CDS 50.0%
!! CTTGATTGATGCGTTGGCTG+GGG + chr5_3:12252347-12252366 Msa0799160:CDS 50.0%
!! CGAAGATGAGCCGTTTTACC+TGG + chr5_3:12252488-12252507 Msa0799160:CDS 50.0%
! CAAATTTTGCAGGCGGTGTC+TGG + chr5_3:12252528-12252547 Msa0799160:CDS 50.0%
TCTCCGGTGGATAACAACCG+CGG - chr5_3:12250252-12250271 None:intergenic 55.0%
CGGTTGTTATCCACCGGAGA+TGG + chr5_3:12250252-12250271 Msa0799160:CDS 55.0%
TCACCGTGCTCATCCATCTC+CGG - chr5_3:12250268-12250287 None:intergenic 55.0%
ACGGTGATGAGTTTCGGCGA+CGG + chr5_3:12250281-12250300 Msa0799160:CDS 55.0%
TGGTAAACCGAGTGCTCCGT+GGG - chr5_3:12250309-12250328 None:intergenic 55.0%
TTGGTAAACCGAGTGCTCCG+TGG - chr5_3:12250310-12250329 None:intergenic 55.0%
! TGCGAGAGAGTGGTAGTGAC+CGG - chr5_3:12250414-12250433 None:intergenic 55.0%
GTGAAGTGGTTGCGAGAGAG+TGG - chr5_3:12250424-12250443 None:intergenic 55.0%
CACAAGGCGATCTTTGGGCT+TGG + chr5_3:12250441-12250460 Msa0799160:CDS 55.0%
CAAGGCGATCTTTGGGCTTG+GGG + chr5_3:12250443-12250462 Msa0799160:CDS 55.0%
GGGTTTGGGGGATGTCCAAA+AGG + chr5_3:12250618-12250637 Msa0799160:CDS 55.0%
! CCGTGTCCCTGGTTCGAAAT+GGG - chr5_3:12250813-12250832 None:intergenic 55.0%
CCCATTTCGAACCAGGGACA+CGG + chr5_3:12250810-12250829 Msa0799160:intron 55.0%
! ACCGTGTCCCTGGTTCGAAA+TGG - chr5_3:12250814-12250833 None:intergenic 55.0%
TGGATGGCAAGCTGTTTGGG+TGG + chr5_3:12251557-12251576 Msa0799160:CDS 55.0%
GGATGGCAAGCTGTTTGGGT+GGG + chr5_3:12251558-12251577 Msa0799160:CDS 55.0%
GATGGCAAGCTGTTTGGGTG+GGG + chr5_3:12251559-12251578 Msa0799160:CDS 55.0%
GGGTGGGGCTATTATGCAGA+TGG + chr5_3:12251574-12251593 Msa0799160:CDS 55.0%
!! ACTGCGTGGCGTTCAAGTTG+TGG + chr5_3:12251780-12251799 Msa0799160:CDS 55.0%
CGAGTGCCCTTATCAGGTGA+TGG + chr5_3:12252078-12252097 Msa0799160:intron 55.0%
!! GAGGGCACCATCACCTGATA+AGG - chr5_3:12252088-12252107 None:intergenic 55.0%
GATGGTGCCCTCTTGAGTTC+TGG + chr5_3:12252096-12252115 Msa0799160:CDS 55.0%
! CTTCTCTAGCTCGCCCCAAT+TGG - chr5_3:12252137-12252156 None:intergenic 55.0%
GGGGCGAGCTAGAGAAGATT+TGG + chr5_3:12252140-12252159 Msa0799160:CDS 55.0%
CACTCCTGTTTCCGTAGCAG+CGG + chr5_3:12252188-12252207 Msa0799160:CDS 55.0%
ACTCCTGTTTCCGTAGCAGC+GGG + chr5_3:12252189-12252208 Msa0799160:CDS 55.0%
CCAGAGTTCTCAGCTAGGAC+GGG + chr5_3:12252302-12252321 Msa0799160:CDS 55.0%
GCCAACGCATCAATCAAGGC+AGG - chr5_3:12252345-12252364 None:intergenic 55.0%
! GCCTTGATTGATGCGTTGGC+TGG + chr5_3:12252345-12252364 Msa0799160:CDS 55.0%
CCCAGCCAACGCATCAATCA+AGG - chr5_3:12252349-12252368 None:intergenic 55.0%
!! TGCGTTGGCTGGGGAAAATC+CGG + chr5_3:12252356-12252375 Msa0799160:CDS 55.0%
CGCCCTGCTGAAACTGAAAC+CGG - chr5_3:12252378-12252397 None:intergenic 55.0%
TTCAGCAGGGCGTGCACATT+CGG + chr5_3:12252386-12252405 Msa0799160:CDS 55.0%
GAAATGTGGGTCTGGGGATC+TGG + chr5_3:12252429-12252448 Msa0799160:CDS 55.0%
CCACCGGAGATGGATGAGCA+CGG + chr5_3:12250262-12250281 Msa0799160:CDS 60.0%
AGTTTCGGCGACGGAACCCA+CGG + chr5_3:12250290-12250309 Msa0799160:CDS 60.0%
AACGAAGCCCAACTGGGTCG+TGG + chr5_3:12250473-12250492 Msa0799160:CDS 60.0%
ACGAAGCCCAACTGGGTCGT+GGG + chr5_3:12250474-12250493 Msa0799160:CDS 60.0%
GGAAAGCCCACGACCCAGTT+GGG - chr5_3:12250483-12250502 None:intergenic 60.0%
TAGACTGGACACCGTGTCCC+TGG - chr5_3:12250824-12250843 None:intergenic 60.0%
CGCAGTTCTTCAACCAGGCG+AGG - chr5_3:12251766-12251785 None:intergenic 60.0%
CGCCTGGTTGAAGAACTGCG+TGG + chr5_3:12251766-12251785 Msa0799160:CDS 60.0%
CGCCACGCAGTTCTTCAACC+AGG - chr5_3:12251771-12251790 None:intergenic 60.0%
CTCCTGTTTCCGTAGCAGCG+GGG + chr5_3:12252190-12252209 Msa0799160:CDS 60.0%
CCCGTCCTAGCTGAGAACTC+TGG - chr5_3:12252305-12252324 None:intergenic 60.0%
AGTTCTCAGCTAGGACGGGC+AGG + chr5_3:12252306-12252325 Msa0799160:CDS 60.0%
GTTCTCAGCTAGGACGGGCA+GGG + chr5_3:12252307-12252326 Msa0799160:CDS 60.0%
CCGGTGGATAACAACCGCGG+CGG - chr5_3:12250249-12250268 None:intergenic 65.0%
CCGCCGCGGTTGTTATCCAC+CGG + chr5_3:12250246-12250265 Msa0799160:CDS 65.0%
CCGTGCTCATCCATCTCCGG+TGG - chr5_3:12250265-12250284 None:intergenic 65.0%
! GACGGAACCCACGGAGCACT+CGG + chr5_3:12250299-12250318 Msa0799160:CDS 65.0%
! GTGGTAGTGACCGGCGTGGA+CGG - chr5_3:12250405-12250424 None:intergenic 65.0%
! GAGAGTGGTAGTGACCGGCG+TGG - chr5_3:12250409-12250428 None:intergenic 65.0%
CGGAAAGCCCACGACCCAGT+TGG - chr5_3:12250484-12250503 None:intergenic 65.0%
AGCCCCGCTGCTACGGAAAC+AGG - chr5_3:12252195-12252214 None:intergenic 65.0%
GTTTCCGTAGCAGCGGGGCT+TGG + chr5_3:12252195-12252214 Msa0799160:CDS 65.0%
AGCGGGGCTTGGCCACTCTT+TGG + chr5_3:12252206-12252225 Msa0799160:CDS 65.0%
GTGGCCAAGCCCCGCTGCTA+CGG - chr5_3:12252202-12252221 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 12250169 12252874 12250169 ID=Msa0799160;Name=Msa0799160
chr5_3 mRNA 12250169 12252874 12250169 ID=Msa0799160-mRNA-1;Parent=Msa0799160;Name=Msa0799160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=34|1|1|1|1|1|3|290|444
chr5_3 exon 12250169 12250724 12250169 ID=Msa0799160-mRNA-1:exon:5003;Parent=Msa0799160-mRNA-1
chr5_3 exon 12251520 12251841 12251520 ID=Msa0799160-mRNA-1:exon:5004;Parent=Msa0799160-mRNA-1
chr5_3 exon 12252094 12252874 12252094 ID=Msa0799160-mRNA-1:exon:5005;Parent=Msa0799160-mRNA-1
chr5_3 five_prime_UTR 12250169 12250202 12250169 ID=Msa0799160-mRNA-1:five_prime_utr;Parent=Msa0799160-mRNA-1
chr5_3 CDS 12250203 12250724 12250203 ID=Msa0799160-mRNA-1:cds;Parent=Msa0799160-mRNA-1
chr5_3 CDS 12251520 12251841 12251520 ID=Msa0799160-mRNA-1:cds;Parent=Msa0799160-mRNA-1
chr5_3 CDS 12252094 12252584 12252094 ID=Msa0799160-mRNA-1:cds;Parent=Msa0799160-mRNA-1
chr5_3 three_prime_UTR 12252585 12252874 12252585 ID=Msa0799160-mRNA-1:three_prime_utr;Parent=Msa0799160-mRNA-1
Gene Sequence

>Msa0799160

ATGTGGCGATCACAGAGAATATTCCGGAACTTCAAACCAAAAACCGCCGCGGTTGTTATCCACCGGAGATGGATGAGCACGGTGATGAGTTTCGGCGACGGAACCCACGGAGCACTCGGTTTACCAACAACCACCGTTGGTATAGGAACACACGCATACGAACCAACTCCAATTCCTTCTTTACCTTCTGATATTGTTACCGTCCACGCCGGTCACTACCACTCTCTCGCAACCACTTCACAAGGCGATCTTTGGGCTTGGGGTAGAAACAACGAAGCCCAACTGGGTCGTGGGCTTTCCGCTAGAGAAACATGGAATGAGCCAAGAAGAGTGGAGGGGTTAGAAAATGTGAAGGTTTGTGGTGCTTTTGCTTCTGGTGTTGTTTCGTGTTGTGTTGGTGATGATGGGTCTGTTTGGGTTTGGGGGATGTCCAAAAGGGGACAGCTTGGACTTGGAAAACATGTTACTGAAGCTGTTGTGCCTTCTAGAGTTGAAGCACTTTCTCGACAAAACGTAGTTAAGGTCTCGTTTGGTTGGGGGCACGCTCTTGCATTAACTGTGGATGGCAAGCTGTTTGGGTGGGGCTATTATGCAGATGGAAGGATAGGGAATATGGGGAATGATCACTTGGAGTCATCTCCATTAGATTCCAGTTCAAGTGCATTTTCAAACAACACACAAATCACGAGTTCAGACCTCGAAGTTGCTGAAAAGAAAGTCTTACAAGGAATGGCAGAGGAGACTAACATGCCAATAATATGGGAACCTCGCCTGGTTGAAGAACTGCGTGGCGTTCAAGTTGTGGACATTGCATGTGGCCTTGATCACTCTTTAGTTCTTTGCCGTGATGGTGCCCTCTTGAGTTCTGGAAGTAACGTGTATGGCCAATTGGGGCGAGCTAGAGAAGATTTGGGATTTTTTCCTGTTGACATAAAGTTCACTCCTGTTTCCGTAGCAGCGGGGCTTGGCCACTCTTTGGCAATATGCCAGCTTGATGAATCAGATAGTAATGTCACTTTGGGGACTACAGATATTGCTTCATGGGGGTGGAACCAGAGTTCTCAGCTAGGACGGGCAGGGCCTGCCTATCTTCCTGCCTTGATTGATGCGTTGGCTGGGGAAAATCCGGTTTCAGTTTCAGCAGGGCGTGCACATTCGGTTGCTCTCACATCAAAAGGAGAAATGTGGGTCTGGGGATCTGGTAAAAATGGTAGACTTGGATTGTCAAGTTCTGTTGACGAAGATGAGCCGTTTTACCTGGATTCCTTAGAAGGTTTTCAAATTTTGCAGGCGGTGTCTGGGTTTGATCATAATCTGGTTCTTGTTGCTGGCTGA
Protein sequence

>Msa0799160

MWRSQRIFRNFKPKTAAVVIHRRWMSTVMSFGDGTHGALGLPTTTVGIGTHAYEPTPIPSLPSDIVTVHAGHYHSLATTSQGDLWAWGRNNEAQLGRGLSARETWNEPRRVEGLENVKVCGAFASGVVSCCVGDDGSVWVWGMSKRGQLGLGKHVTEAVVPSRVEALSRQNVVKVSFGWGHALALTVDGKLFGWGYYADGRIGNMGNDHLESSPLDSSSSAFSNNTQITSSDLEVAEKKVLQGMAEETNMPIIWEPRLVEELRGVQVVDIACGLDHSLVLCRDGALLSSGSNVYGQLGRAREDLGFFPVDIKFTPVSVAAGLGHSLAICQLDESDSNVTLGTTDIASWGWNQSSQLGRAGPAYLPALIDALAGENPVSVSAGRAHSVALTSKGEMWVWGSGKNGRLGLSSSVDEDEPFYLDSLEGFQILQAVSGFDHNLVLVAG*