AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0812240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0812240 MtrunA17_Chr5g0428861 98.438 192 3 0 1 192 1 192 1.51e-139 387
Msa0812240 MtrunA17_Chr3g0128201 87.500 192 24 0 1 192 1 192 6.62e-126 352
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0812240 AT1G33140.1 84.456 193 29 1 1 192 1 193 4.41e-120 338
Msa0812240 AT1G33120.1 84.456 193 29 1 1 192 1 193 4.41e-120 338
Msa0812240 AT4G10450.1 82.383 193 33 1 1 192 1 193 6.07e-117 330
Msa0812240 AT4G10450.2 81.928 166 29 1 1 165 1 166 3.81e-99 284

Find 72 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGAGTGAAATCATTGTTAT+TGG 0.188764 5_3:+42164841 None:intergenic
GGTGAAGGATGAGTTGATTT+TGG 0.219023 5_3:-42162048 Msa0812240:CDS
AACTGCAGACCTGGTTAATA+AGG 0.222453 5_3:+42161988 None:intergenic
AACATTGATATTAGTTTGAT+TGG 0.254828 5_3:-42161758 Msa0812240:three_prime_UTR
AGAATAGATTATCGGCAAAT+AGG 0.332688 5_3:-42161781 Msa0812240:exon
GTTAATAAGGGCACAAGATC+TGG 0.336166 5_3:+42162001 None:intergenic
TGTTATTGGCCCACTCTAAT+CGG 0.350392 5_3:+42164855 None:intergenic
GCTTTCTGCGTGGGTGAATA+GGG 0.351660 5_3:+42164782 None:intergenic
TGAAGGTCCACGAGGTAAAT+TGG 0.356555 5_3:-42164640 Msa0812240:CDS
AGCTTTCTGCGTGGGTGAAT+AGG 0.360184 5_3:+42164781 None:intergenic
CTATTGATATACATACATTA+TGG 0.361780 5_3:-42161649 Msa0812240:three_prime_UTR
GTCGGGAATGTCCATTGTTT+CGG 0.365385 5_3:+42164701 None:intergenic
CTAAAGGTTGAAGCTTGGTT+TGG 0.367033 5_3:-42164552 Msa0812240:CDS
TTAGTAATACTAGCATTAAT+AGG 0.377704 5_3:+42164417 None:intergenic
TCTGCAAAACTTTGTAGAAC+AGG 0.385733 5_3:-42161562 Msa0812240:three_prime_UTR
TGCTCTCTCTCGCCGCCGGC+GGG 0.391506 5_3:+42164745 None:intergenic
TTGAAGCTTGGTTTGGATCA+AGG 0.391941 5_3:-42164545 Msa0812240:CDS
AAATGTAAATGACAAGTATT+GGG 0.409074 5_3:+42161529 None:intergenic
ACCTTCAACCTCGATGAGTT+TGG 0.410571 5_3:+42164656 None:intergenic
ATTGCTGAAGAATAGATTAT+CGG 0.412035 5_3:-42161789 Msa0812240:exon
TCTCATCACTGGTGTTACTA+AGG 0.424742 5_3:-42164478 Msa0812240:CDS
AAAATGTAAATGACAAGTAT+TGG 0.430103 5_3:+42161528 None:intergenic
ATTGAGATTCGTAACTTCCT+TGG 0.437199 5_3:-42164381 Msa0812240:CDS
GTTATTGGCCCACTCTAATC+GGG 0.447931 5_3:+42164856 None:intergenic
GACTTTGATGCTTACTCCGT+CGG 0.453982 5_3:+42164683 None:intergenic
AGATACCATCAAGGAACTTC+CGG 0.458260 5_3:+42161832 None:intergenic
CAGCTAATTACAGACGAAGA+AGG 0.461385 5_3:-42164585 Msa0812240:CDS
CACTATCCTTCGATCTGAAA+AGG 0.468016 5_3:-42162069 Msa0812240:CDS
TTGCTCTCTCTCGCCGCCGG+CGG 0.473354 5_3:+42164744 None:intergenic
TTGCAGACAAGGAGTTATGA+TGG 0.476759 5_3:+42161578 None:intergenic
GATATCCGGAAGTTCCTTGA+TGG 0.481710 5_3:-42161837 Msa0812240:CDS
ATCTTTCAGAAAGATAACTT+TGG 0.496875 5_3:+42161693 None:intergenic
CGCCGCCGGCGGGAATGCAG+AGG 0.499664 5_3:+42164755 None:intergenic
AGAAGCTAAAGGTTGAAGCT+TGG 0.507094 5_3:-42164557 Msa0812240:CDS
CATTGTTTCGGAAGAGAGGA+TGG 0.507424 5_3:+42164713 None:intergenic
TGCAGACAAGGAGTTATGAT+GGG 0.511384 5_3:+42161579 None:intergenic
ATGTTAAGAACAAAGATATC+CGG 0.514793 5_3:-42161851 Msa0812240:CDS
ATCTTGCTCTCTCTCGCCGC+CGG 0.519718 5_3:+42164741 None:intergenic
CATGTTGATAATCTCATCAC+TGG 0.524737 5_3:-42164489 Msa0812240:CDS
AGCTAATTACAGACGAAGAA+GGG 0.525662 5_3:-42164584 Msa0812240:CDS
CTCATCACTGGTGTTACTAA+GGG 0.526252 5_3:-42164477 Msa0812240:CDS
AGAAAAGTTGACATGCTTAA+CGG 0.531676 5_3:-42162095 Msa0812240:CDS
ACTGCAGACCTGGTTAATAA+GGG 0.534157 5_3:+42161989 None:intergenic
CATCCTCTCTTCCGAAACAA+TGG 0.543487 5_3:-42164712 Msa0812240:CDS
GCCAAACTCATCGAGGTTGA+AGG 0.549542 5_3:-42164657 Msa0812240:CDS
TCCACGAGGTAAATTGGTGC+GGG 0.549581 5_3:-42164634 Msa0812240:CDS
ACTTTGATGCTTACTCCGTC+GGG 0.550847 5_3:+42164684 None:intergenic
ATCTTGTGCCCTTATTAACC+AGG 0.554399 5_3:-42161997 Msa0812240:intron
ACTTTGGCAACACAAAATTG+TGG 0.560568 5_3:+42161709 None:intergenic
TGTCCATTGTTTCGGAAGAG+AGG 0.563232 5_3:+42164709 None:intergenic
CGCCTCTGCATTCCCGCCGG+CGG 0.564821 5_3:-42164757 Msa0812240:five_prime_UTR
GGTATCTATGTTAGCGAGAG+GGG 0.570010 5_3:-42161816 Msa0812240:CDS
CCTTCGATCTGAAAAGGTGA+AGG 0.575546 5_3:-42162063 Msa0812240:CDS
CAACCTCGATGAGTTTGGCG+TGG 0.580509 5_3:+42164661 None:intergenic
CTTCGCCTCTGCATTCCCGC+CGG 0.588832 5_3:-42164760 Msa0812240:five_prime_UTR
AGAGGCGAAGCTTTCTGCGT+GGG 0.588920 5_3:+42164773 None:intergenic
AAACAAAACGCATCTTGTAA+CGG 0.594741 5_3:+42164451 None:intergenic
ATCTAAGACCCGATTAGAGT+GGG 0.600388 5_3:-42164864 Msa0812240:five_prime_UTR
AGCAGTACGAATAGCAGCAG+AGG 0.600567 5_3:+42164518 None:intergenic
CGCTAACATAGATACCATCA+AGG 0.600705 5_3:+42161823 None:intergenic
GTCCACGAGGTAAATTGGTG+CGG 0.602536 5_3:-42164635 Msa0812240:CDS
TATCTAAGACCCGATTAGAG+TGG 0.612625 5_3:-42164865 Msa0812240:five_prime_UTR
AGAAGGGAAGAAGAAGCTAA+AGG 0.615201 5_3:-42164568 Msa0812240:CDS
TGGTATCTATGTTAGCGAGA+GGG 0.617657 5_3:-42161817 Msa0812240:CDS
GCAGACAAGGAGTTATGATG+GGG 0.628361 5_3:+42161580 None:intergenic
TCCCGCACCAATTTACCTCG+TGG 0.646235 5_3:+42164633 None:intergenic
ATGGTATCTATGTTAGCGAG+AGG 0.648406 5_3:-42161818 Msa0812240:CDS
CAGAGGCGAAGCTTTCTGCG+TGG 0.685721 5_3:+42164772 None:intergenic
CTGTAATTAGCTGAAAATCG+AGG 0.687812 5_3:+42164595 None:intergenic
GAAACAATGGACATTCCCGA+CGG 0.716770 5_3:-42164699 Msa0812240:CDS
AGTCCACGCCAAACTCATCG+AGG 0.736394 5_3:-42164664 Msa0812240:CDS
ATCGAGGTTGAAGGTCCACG+AGG 0.744735 5_3:-42164648 Msa0812240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTATTTTTTTTAATT+TGG - chr5_3:42163635-42163654 Msa0812240:intron 0.0%
!!! TTTTAATTTCGTTTTTTTTT+TGG - chr5_3:42162014-42162033 Msa0812240:CDS 10.0%
!! ATCAAATTTAAATTTGTATT+TGG - chr5_3:42164215-42164234 Msa0812240:intron 10.0%
!! ATTATAAGATTATTCATGAT+TGG - chr5_3:42162256-42162275 Msa0812240:intron 15.0%
!! TTATAAGATTATTCATGATT+GGG - chr5_3:42162257-42162276 Msa0812240:intron 15.0%
!! TTATTTGAATGAAATTAGTT+TGG - chr5_3:42162292-42162311 Msa0812240:intron 15.0%
!!! TTGATTTTTTACTAAGAAAA+AGG - chr5_3:42163185-42163204 Msa0812240:intron 15.0%
!!! TGATTTTTTACTAAGAAAAA+GGG - chr5_3:42163186-42163205 Msa0812240:intron 15.0%
!! ACAAAATCTAAAAATACATA+CGG + chr5_3:42163540-42163559 None:intergenic 15.0%
!! TTAGTAATACTAGCATTAAT+AGG + chr5_3:42161937-42161956 None:intergenic 20.0%
!! AGCAATATAACTACTAAAAT+GGG + chr5_3:42162141-42162160 None:intergenic 20.0%
!! AAGCAATATAACTACTAAAA+TGG + chr5_3:42162142-42162161 None:intergenic 20.0%
!!! CTTATTTTTTTTCAGCTTAT+AGG + chr5_3:42162481-42162500 None:intergenic 20.0%
!! TATACACCAAATAAAAAACA+TGG + chr5_3:42162615-42162634 None:intergenic 20.0%
!!! ATTTTCCAAAATCTTAGTTT+GGG - chr5_3:42163099-42163118 Msa0812240:intron 20.0%
!!! ACAATATTTTGAATAAAAGG+TGG + chr5_3:42163317-42163336 None:intergenic 20.0%
!!! TGCACAATATTTTGAATAAA+AGG + chr5_3:42163320-42163339 None:intergenic 20.0%
!! TGTTTCACAAAAATTATTAG+AGG + chr5_3:42163492-42163511 None:intergenic 20.0%
!! TACAAGACTATTTATGTAAT+AGG + chr5_3:42163569-42163588 None:intergenic 20.0%
!!! CTTTATTTTTTTTTTCCCAA+GGG - chr5_3:42163733-42163752 Msa0812240:intron 20.0%
!!! TTCTTATATTTTTTTCCCTT+GGG + chr5_3:42163751-42163770 None:intergenic 20.0%
!! AAATCTATATTAAGCAGAAT+AGG - chr5_3:42164091-42164110 Msa0812240:intron 20.0%
!! TTAAAATCAAAACAAGAGAA+TGG + chr5_3:42164429-42164448 None:intergenic 20.0%
!! ATTAAAATTAAAACCTGAGA+CGG + chr5_3:42164452-42164471 None:intergenic 20.0%
!!! AACATTGATATTAGTTTGAT+TGG - chr5_3:42164593-42164612 Msa0812240:CDS 20.0%
!! CTATTGATATACATACATTA+TGG - chr5_3:42164702-42164721 Msa0812240:CDS 20.0%
!!! ATGGAATATTCTTTTTGTTT+TGG - chr5_3:42164721-42164740 Msa0812240:CDS 20.0%
!! AAATGTAAATGACAAGTATT+GGG + chr5_3:42164825-42164844 None:intergenic 20.0%
!! AAAATGTAAATGACAAGTAT+TGG + chr5_3:42164826-42164845 None:intergenic 20.0%
!!! TGTTGTTGTGATTTTTGAAA+TGG - chr5_3:42162096-42162115 Msa0812240:CDS 25.0%
!!! GTTGTTGTGATTTTTGAAAT+GGG - chr5_3:42162097-42162116 Msa0812240:CDS 25.0%
!!! TTTTGAAATGGGTTGTTTTT+TGG - chr5_3:42162108-42162127 Msa0812240:intron 25.0%
! TTGTTTAATGCATATGCTTT+AGG - chr5_3:42162183-42162202 Msa0812240:intron 25.0%
! TGTTTAATGCATATGCTTTA+GGG - chr5_3:42162184-42162203 Msa0812240:intron 25.0%
! CAAAAATAAGTCAATCCAAA+CGG - chr5_3:42162454-42162473 Msa0812240:intron 25.0%
! AAAAATAAGTCAATCCAAAC+GGG - chr5_3:42162455-42162474 Msa0812240:intron 25.0%
!!! GAAGATCCATGTTTTTTATT+TGG - chr5_3:42162606-42162625 Msa0812240:intron 25.0%
!! GATTTTCCAAAATCTTAGTT+TGG - chr5_3:42163098-42163117 Msa0812240:intron 25.0%
!!! TTTTCCAAAATCTTAGTTTG+GGG - chr5_3:42163100-42163119 Msa0812240:intron 25.0%
! GTTATGAATGGAAAACAATT+CGG + chr5_3:42163258-42163277 None:intergenic 25.0%
!!! CATGTATTTTTTTACTTGAG+AGG - chr5_3:42163418-42163437 Msa0812240:intron 25.0%
! TGTGAAACAAAAACATAGTT+TGG - chr5_3:42163503-42163522 Msa0812240:intron 25.0%
!!! GCTTTATTTTTTTTTTCCCA+AGG - chr5_3:42163732-42163751 Msa0812240:intron 25.0%
!!! CTTCTTATATTTTTTTCCCT+TGG + chr5_3:42163752-42163771 None:intergenic 25.0%
! AGGGAAAAAAATATAAGAAG+TGG - chr5_3:42163752-42163771 Msa0812240:intron 25.0%
! AAGATTATTGTTGGAATGAA+TGG - chr5_3:42164120-42164139 Msa0812240:intron 25.0%
! GAAAGAGTCTAAAAACTAAA+AGG + chr5_3:42164192-42164211 None:intergenic 25.0%
! ATGTTAAGAACAAAGATATC+CGG - chr5_3:42164500-42164519 Msa0812240:CDS 25.0%
! ATTGCTGAAGAATAGATTAT+CGG - chr5_3:42164562-42164581 Msa0812240:CDS 25.0%
!!! TGGAAAAGTATTAGCTTTTT+TGG - chr5_3:42164613-42164632 Msa0812240:CDS 25.0%
! ATCTTTCAGAAAGATAACTT+TGG + chr5_3:42164661-42164680 None:intergenic 25.0%
!!! ATTAGGGTTTTAGAAAAGAG+GGG + chr5_3:42161542-42161561 None:intergenic 30.0%
!!! GATTAGGGTTTTAGAAAAGA+GGG + chr5_3:42161543-42161562 None:intergenic 30.0%
!!! TGAATAGGGTTTTAGGATTA+GGG + chr5_3:42161558-42161577 None:intergenic 30.0%
AAACAAAACGCATCTTGTAA+CGG + chr5_3:42161903-42161922 None:intergenic 30.0%
TAACTTCCTTGGTGAAAAAA+AGG - chr5_3:42161981-42162000 Msa0812240:intron 30.0%
!!! AAAGTACCTTTTTTTCACCA+AGG + chr5_3:42161990-42162009 None:intergenic 30.0%
!! TTTGTAATTGCTTAGTTCTG+AGG - chr5_3:42162214-42162233 Msa0812240:intron 30.0%
!!! TAGCTTTGGTGTTTTTGTTT+TGG - chr5_3:42162654-42162673 Msa0812240:intron 30.0%
! AATCTAAAGCATGTAAGAAG+TGG - chr5_3:42162735-42162754 Msa0812240:intron 30.0%
ACATGTTTATCTTACTTGAG+AGG - chr5_3:42162987-42163006 Msa0812240:intron 30.0%
GTAATAGAGCAAATAAACAG+TGG + chr5_3:42163470-42163489 None:intergenic 30.0%
AGGCATTTGTCTGAATTATT+TGG - chr5_3:42163849-42163868 Msa0812240:intron 30.0%
ATCTAATCTTACAGTACACA+TGG + chr5_3:42163973-42163992 None:intergenic 30.0%
! GATCTGTTACCTTTAAGAAT+TGG - chr5_3:42163990-42164009 Msa0812240:intron 30.0%
! TTACCTTTAAGAATTGGAAC+TGG - chr5_3:42163996-42164015 Msa0812240:intron 30.0%
AGGTTTGACAAGATTATTGT+TGG - chr5_3:42164111-42164130 Msa0812240:intron 30.0%
TGTTAGTTAACACAGTAAGA+TGG - chr5_3:42164162-42164181 Msa0812240:intron 30.0%
AGAAAAGTTGACATGCTTAA+CGG - chr5_3:42164256-42164275 Msa0812240:intron 30.0%
AGAATAGATTATCGGCAAAT+AGG - chr5_3:42164570-42164589 Msa0812240:CDS 30.0%
GCGAGTGAAATCATTGTTAT+TGG + chr5_3:42161513-42161532 None:intergenic 35.0%
!!! GGATTAGGGTTTTAGAAAAG+AGG + chr5_3:42161544-42161563 None:intergenic 35.0%
!!! GTGAATAGGGTTTTAGGATT+AGG + chr5_3:42161559-42161578 None:intergenic 35.0%
CTGTAATTAGCTGAAAATCG+AGG + chr5_3:42161759-42161778 None:intergenic 35.0%
AGCTAATTACAGACGAAGAA+GGG - chr5_3:42161767-42161786 Msa0812240:three_prime_UTR 35.0%
!! CATGTTGATAATCTCATCAC+TGG - chr5_3:42161862-42161881 Msa0812240:intron 35.0%
ATTGAGATTCGTAACTTCCT+TGG - chr5_3:42161970-42161989 Msa0812240:intron 35.0%
! TTTGGTGTATAGTGTGTGAT+TGG - chr5_3:42162624-42162643 Msa0812240:intron 35.0%
TATGTCATCAGGGTTAAGAA+TGG + chr5_3:42162805-42162824 None:intergenic 35.0%
TGTGCATTTCACATGAATTC+TGG - chr5_3:42162903-42162922 Msa0812240:intron 35.0%
GTGTAATCAAGTCAGAAACA+TGG + chr5_3:42163015-42163034 None:intergenic 35.0%
!! GGGCTTCAGTATTTTTGTTT+TGG - chr5_3:42163120-42163139 Msa0812240:intron 35.0%
AAGAAAAAGGGTCTAAGAAG+TGG - chr5_3:42163198-42163217 Msa0812240:intron 35.0%
AATACCTTGATATGTCGTCA+GGG + chr5_3:42163280-42163299 None:intergenic 35.0%
AAATACCTTGATATGTCGTC+AGG + chr5_3:42163281-42163300 None:intergenic 35.0%
! TATCAAGGTATTTGTCTGAG+AGG - chr5_3:42163288-42163307 Msa0812240:intron 35.0%
!!! TACTTGTTTTGTGACTTGAG+AGG - chr5_3:42163587-42163606 Msa0812240:intron 35.0%
GATGACGAGTCTGAATTATT+TGG - chr5_3:42163801-42163820 Msa0812240:intron 35.0%
GAACCAGTTCCAATTCTTAA+AGG + chr5_3:42164002-42164021 None:intergenic 35.0%
! TTAAGAATTGGAACTGGTTC+TGG - chr5_3:42164002-42164021 Msa0812240:intron 35.0%
! TAAGAATTGGAACTGGTTCT+GGG - chr5_3:42164003-42164022 Msa0812240:intron 35.0%
!!! AAGGATGAGTTGATTTTGGA+TGG - chr5_3:42164307-42164326 Msa0812240:intron 35.0%
!!! GTTTTGATTTTAACCGTCTC+AGG - chr5_3:42164436-42164455 Msa0812240:CDS 35.0%
ACTTTGGCAACACAAAATTG+TGG + chr5_3:42164645-42164664 None:intergenic 35.0%
! TCTACAAAGTTTTGCAGACA+AGG + chr5_3:42164787-42164806 None:intergenic 35.0%
TCTGCAAAACTTTGTAGAAC+AGG - chr5_3:42164789-42164808 Msa0812240:five_prime_UTR 35.0%
! TATCTAAGACCCGATTAGAG+TGG - chr5_3:42161486-42161505 Msa0812240:three_prime_UTR 40.0%
! ATCTAAGACCCGATTAGAGT+GGG - chr5_3:42161487-42161506 Msa0812240:three_prime_UTR 40.0%
TGTTATTGGCCCACTCTAAT+CGG + chr5_3:42161499-42161518 None:intergenic 40.0%
CAGCTAATTACAGACGAAGA+AGG - chr5_3:42161766-42161785 Msa0812240:three_prime_UTR 40.0%
AGAAGGGAAGAAGAAGCTAA+AGG - chr5_3:42161783-42161802 Msa0812240:exon 40.0%
AGAAGCTAAAGGTTGAAGCT+TGG - chr5_3:42161794-42161813 Msa0812240:exon 40.0%
CTAAAGGTTGAAGCTTGGTT+TGG - chr5_3:42161799-42161818 Msa0812240:CDS 40.0%
!! TTGAAGCTTGGTTTGGATCA+AGG - chr5_3:42161806-42161825 Msa0812240:CDS 40.0%
!! TCTCATCACTGGTGTTACTA+AGG - chr5_3:42161873-42161892 Msa0812240:intron 40.0%
!! CTCATCACTGGTGTTACTAA+GGG - chr5_3:42161874-42161893 Msa0812240:intron 40.0%
AAACTGCTCTGACAAGCTTA+TGG + chr5_3:42162335-42162354 None:intergenic 40.0%
! TGATTGGTGGTTTGTAGCTT+TGG - chr5_3:42162640-42162659 Msa0812240:intron 40.0%
TTAACCCTGATGACATATCG+AGG - chr5_3:42162808-42162827 Msa0812240:intron 40.0%
AATGCCTCGATATGTCATCA+GGG + chr5_3:42162815-42162834 None:intergenic 40.0%
AAATGCCTCGATATGTCATC+AGG + chr5_3:42162816-42162835 None:intergenic 40.0%
ACGACATTTGGAAGGAAAAG+TGG + chr5_3:42162852-42162871 None:intergenic 40.0%
TGAAATGCACGACATTTGGA+AGG + chr5_3:42162860-42162879 None:intergenic 40.0%
CATGTGAAATGCACGACATT+TGG + chr5_3:42162864-42162883 None:intergenic 40.0%
GCACAACAATCACAATCACA+CGG + chr5_3:42163065-42163084 None:intergenic 40.0%
! GAAGCCCCAAACTAAGATTT+TGG + chr5_3:42163107-42163126 None:intergenic 40.0%
TATGTCGTCAGGGTTATGAA+TGG + chr5_3:42163270-42163289 None:intergenic 40.0%
ATAACCCTGACGACATATCA+AGG - chr5_3:42163273-42163292 Msa0812240:intron 40.0%
GTGTGATTGATGTGCATTTC+AGG - chr5_3:42163664-42163683 Msa0812240:intron 40.0%
TTAATCCTGACGACATATCG+AGG - chr5_3:42163829-42163848 Msa0812240:intron 40.0%
TTAATCCTGACGACATATCG+AGG - chr5_3:42163829-42163848 Msa0812240:intron 40.0%
! TATCGAGGCATTTATCTGAG+AGG - chr5_3:42163892-42163911 Msa0812240:intron 40.0%
! TCAACTTTTCTCACCTGCAA+CGG + chr5_3:42164247-42164266 None:intergenic 40.0%
CACTATCCTTCGATCTGAAA+AGG - chr5_3:42164282-42164301 Msa0812240:intron 40.0%
!!! GGTGAAGGATGAGTTGATTT+TGG - chr5_3:42164303-42164322 Msa0812240:intron 40.0%
! GTTAATAAGGGCACAAGATC+TGG + chr5_3:42164353-42164372 None:intergenic 40.0%
ATCTTGTGCCCTTATTAACC+AGG - chr5_3:42164354-42164373 Msa0812240:intron 40.0%
ACTGCAGACCTGGTTAATAA+GGG + chr5_3:42164365-42164384 None:intergenic 40.0%
AACTGCAGACCTGGTTAATA+AGG + chr5_3:42164366-42164385 None:intergenic 40.0%
GCACACATGTAAGTATACAC+AGG + chr5_3:42164393-42164412 None:intergenic 40.0%
AGATACCATCAAGGAACTTC+CGG + chr5_3:42164522-42164541 None:intergenic 40.0%
CGCTAACATAGATACCATCA+AGG + chr5_3:42164531-42164550 None:intergenic 40.0%
ATGGTATCTATGTTAGCGAG+AGG - chr5_3:42164533-42164552 Msa0812240:CDS 40.0%
TGGTATCTATGTTAGCGAGA+GGG - chr5_3:42164534-42164553 Msa0812240:CDS 40.0%
TGCAGACAAGGAGTTATGAT+GGG + chr5_3:42164775-42164794 None:intergenic 40.0%
TTGCAGACAAGGAGTTATGA+TGG + chr5_3:42164776-42164795 None:intergenic 40.0%
GTTATTGGCCCACTCTAATC+GGG + chr5_3:42161498-42161517 None:intergenic 45.0%
CATTGTTTCGGAAGAGAGGA+TGG + chr5_3:42161641-42161660 None:intergenic 45.0%
CATCCTCTCTTCCGAAACAA+TGG - chr5_3:42161639-42161658 Msa0812240:three_prime_UTR 45.0%
TGTCCATTGTTTCGGAAGAG+AGG + chr5_3:42161645-42161664 None:intergenic 45.0%
GTCGGGAATGTCCATTGTTT+CGG + chr5_3:42161653-42161672 None:intergenic 45.0%
GAAACAATGGACATTCCCGA+CGG - chr5_3:42161652-42161671 Msa0812240:three_prime_UTR 45.0%
ACTTTGATGCTTACTCCGTC+GGG + chr5_3:42161670-42161689 None:intergenic 45.0%
GACTTTGATGCTTACTCCGT+CGG + chr5_3:42161671-42161690 None:intergenic 45.0%
ACCTTCAACCTCGATGAGTT+TGG + chr5_3:42161698-42161717 None:intergenic 45.0%
TGAAGGTCCACGAGGTAAAT+TGG - chr5_3:42161711-42161730 Msa0812240:three_prime_UTR 45.0%
TTTCAGCTTATAGGCCCGTT+TGG + chr5_3:42162472-42162491 None:intergenic 45.0%
GGTGTATAGTGTGTGATTGG+TGG - chr5_3:42162627-42162646 Msa0812240:intron 45.0%
AAATGCCTCGATATGTCGTC+AGG + chr5_3:42163837-42163856 None:intergenic 45.0%
AAATGCCTCGATATGTCGTC+AGG + chr5_3:42163837-42163856 None:intergenic 45.0%
TATTTGGTTGTCACCGTTGC+AGG - chr5_3:42164231-42164250 Msa0812240:intron 45.0%
! CCTTCACCTTTTCAGATCGA+AGG + chr5_3:42164291-42164310 None:intergenic 45.0%
CCTTCGATCTGAAAAGGTGA+AGG - chr5_3:42164288-42164307 Msa0812240:intron 45.0%
GATATCCGGAAGTTCCTTGA+TGG - chr5_3:42164514-42164533 Msa0812240:CDS 45.0%
GGTATCTATGTTAGCGAGAG+GGG - chr5_3:42164535-42164554 Msa0812240:CDS 45.0%
GCAGACAAGGAGTTATGATG+GGG + chr5_3:42164774-42164793 None:intergenic 45.0%
!!! ATTTTATTTGAAATTTTTAA+TGG - chr5_3:42164679-42164698 Msa0812240:CDS 5.0%
! GCGTGGGTGAATAGGGTTTT+AGG + chr5_3:42161565-42161584 None:intergenic 50.0%
GCTTTCTGCGTGGGTGAATA+GGG + chr5_3:42161572-42161591 None:intergenic 50.0%
AGCTTTCTGCGTGGGTGAAT+AGG + chr5_3:42161573-42161592 None:intergenic 50.0%
GCCAAACTCATCGAGGTTGA+AGG - chr5_3:42161694-42161713 Msa0812240:three_prime_UTR 50.0%
GTCCACGAGGTAAATTGGTG+CGG - chr5_3:42161716-42161735 Msa0812240:three_prime_UTR 50.0%
TCCACGAGGTAAATTGGTGC+GGG - chr5_3:42161717-42161736 Msa0812240:three_prime_UTR 50.0%
AGCAGTACGAATAGCAGCAG+AGG + chr5_3:42161836-42161855 None:intergenic 50.0%
ACAGGAAAGAACTGCAGACC+TGG + chr5_3:42164375-42164394 None:intergenic 50.0%
AGAGGCGAAGCTTTCTGCGT+GGG + chr5_3:42161581-42161600 None:intergenic 55.0%
AGTCCACGCCAAACTCATCG+AGG - chr5_3:42161687-42161706 Msa0812240:three_prime_UTR 55.0%
CAACCTCGATGAGTTTGGCG+TGG + chr5_3:42161693-42161712 None:intergenic 55.0%
ATCGAGGTTGAAGGTCCACG+AGG - chr5_3:42161703-42161722 Msa0812240:three_prime_UTR 55.0%
TCCCGCACCAATTTACCTCG+TGG + chr5_3:42161721-42161740 None:intergenic 55.0%
CAGAGGCGAAGCTTTCTGCG+TGG + chr5_3:42161582-42161601 None:intergenic 60.0%
ATCTTGCTCTCTCTCGCCGC+CGG + chr5_3:42161613-42161632 None:intergenic 60.0%
CTTCGCCTCTGCATTCCCGC+CGG - chr5_3:42161591-42161610 Msa0812240:three_prime_UTR 65.0%
TTGCTCTCTCTCGCCGCCGG+CGG + chr5_3:42161610-42161629 None:intergenic 70.0%
CGCCTCTGCATTCCCGCCGG+CGG - chr5_3:42161594-42161613 Msa0812240:three_prime_UTR 75.0%
TGCTCTCTCTCGCCGCCGGC+GGG + chr5_3:42161609-42161628 None:intergenic 75.0%
! CGCCGCCGGCGGGAATGCAG+AGG + chr5_3:42161599-42161618 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 42161482 42164891 42161482 ID=Msa0812240;Name=Msa0812240
chr5_3 mRNA 42161482 42164891 42161482 ID=Msa0812240-mRNA-1;Parent=Msa0812240;Name=Msa0812240-mRNA-1;_AED=0.06;_eAED=0.06;_QI=149|1|1|1|1|1|3|315|192
chr5_3 exon 42161482 42161880 42161482 ID=Msa0812240-mRNA-1:exon:12954;Parent=Msa0812240-mRNA-1
chr5_3 exon 42161998 42162120 42161998 ID=Msa0812240-mRNA-1:exon:12953;Parent=Msa0812240-mRNA-1
chr5_3 exon 42164371 42164891 42164371 ID=Msa0812240-mRNA-1:exon:12952;Parent=Msa0812240-mRNA-1
chr5_3 five_prime_UTR 42164743 42164891 42164743 ID=Msa0812240-mRNA-1:five_prime_utr;Parent=Msa0812240-mRNA-1
chr5_3 CDS 42164371 42164742 42164371 ID=Msa0812240-mRNA-1:cds;Parent=Msa0812240-mRNA-1
chr5_3 CDS 42161998 42162120 42161998 ID=Msa0812240-mRNA-1:cds;Parent=Msa0812240-mRNA-1
chr5_3 CDS 42161797 42161880 42161797 ID=Msa0812240-mRNA-1:cds;Parent=Msa0812240-mRNA-1
chr5_3 three_prime_UTR 42161482 42161796 42161482 ID=Msa0812240-mRNA-1:three_prime_utr;Parent=Msa0812240-mRNA-1
Gene Sequence

>Msa0812240

ATGAAAACCATCCTCTCTTCCGAAACAATGGACATTCCCGACGGAGTAAGCATCAAAGTCCACGCCAAACTCATCGAGGTTGAAGGTCCACGAGGTAAATTGGTGCGGGATTTCAAGCATTTGAACCTCGATTTTCAGCTAATTACAGACGAAGAAGGGAAGAAGAAGCTAAAGGTTGAAGCTTGGTTTGGATCAAGGAAAACCTCTGCTGCTATTCGTACTGCTCTTAGTCATGTTGATAATCTCATCACTGGTGTTACTAAGGGTTACCGTTACAAGATGCGTTTTGTTTATGCACATTTTCCTATTAATGCTAGTATTACTAATACGAATTCTGCTATTGAGATTCGTAACTTCCTTGGTGAAAAAAAGGTGAGAAAAGTTGACATGCTTAACGGAGTCACTATCCTTCGATCTGAAAAGGTGAAGGATGAGTTGATTTTGGATGGAAATGACATTGAACTTGTCTCCAGATCTTGTGCCCTTATTAACCAGAAATGTCATGTTAAGAACAAAGATATCCGGAAGTTCCTTGATGGTATCTATGTTAGCGAGAGGGGAACAATTGCTGAAGAATAG
Protein sequence

>Msa0812240

MKTILSSETMDIPDGVSIKVHAKLIEVEGPRGKLVRDFKHLNLDFQLITDEEGKKKLKVEAWFGSRKTSAAIRTALSHVDNLITGVTKGYRYKMRFVYAHFPINASITNTNSAIEIRNFLGEKKVRKVDMLNGVTILRSEKVKDELILDGNDIELVSRSCALINQKCHVKNKDIRKFLDGIYVSERGTIAEE*