AlfalfaGEDB | Msa0813640

M. sativa cultivar zhongmu-4 / Msa0813640

KEGG: K21374
GO: -
PFAM: PF00201.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0813640	GAU31028.1	72.701	348	50	3	6	315	3	343	4.48e-178	509

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0813640	sp\|O64732\|U87A1_ARATH	52.321	237	100	6	77	310	147	373	3.49e-73	236
Msa0813640	sp\|O64732\|U87A1_ARATH	62.500	64	21	2	16	79	1	61	2.19e-16	83.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0813640	A0A2Z6MGK6	72.701	348	50	3	6	315	3	343	2.14e-178	509

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0193120	Msa0813640	0.811956	5.401103e-51	-8.615850e-47
Msa0241640	Msa0813640	0.809538	1.800743e-50	-8.615850e-47
Msa0358170	Msa0813640	0.807932	3.969639e-50	-8.615850e-47
Msa0395960	Msa0813640	0.820096	8.214126e-53	-8.615850e-47
Msa0734740	Msa0813640	0.825332	4.966258e-54	-8.615850e-47
Msa0734750	Msa0813640	0.838845	2.257977e-57	-8.615850e-47
Msa0743720	Msa0813640	0.800332	1.514795e-48	-8.615850e-47
Msa0813640	Msa0813670	0.802608	5.175777e-49	-8.615850e-47
Msa0813640	Msa0813720	0.842029	3.318123e-58	-8.615850e-47
Msa0813640	Msa0813740	0.875959	2.042429e-68	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0813640	MtrunA17_Chr5g0431441	95.217	230	11	0	81	310	173	402	6.77e-163	463
Msa0813640	MtrunA17_Chr5g0431441	77.215	79	18	0	12	90	18	96	1.40e-33	129
Msa0813640	MtrunA17_Chr5g0431431	53.216	342	116	5	5	310	3	336	2.21e-121	354
Msa0813640	MtrunA17_Chr5g0432731	60.338	237	91	2	81	315	3	238	7.90e-97	289
Msa0813640	MtrunA17_Chr3g0106231	58.190	232	93	3	81	310	159	388	1.23e-90	279
Msa0813640	MtrunA17_Chr3g0106231	53.261	92	32	3	1	90	5	87	3.79e-21	94.0
Msa0813640	MtrunA17_Chr3g0106251	57.895	228	92	3	83	310	7	230	1.28e-90	274
Msa0813640	MtrunA17_Chr5g0431361	52.212	226	105	2	84	309	165	387	1.30e-75	240
Msa0813640	MtrunA17_Chr5g0431361	60.000	75	25	3	12	84	12	83	5.24e-20	90.5
Msa0813640	MtrunA17_Chr5g0431421	47.280	239	119	3	75	311	151	384	1.29e-70	227

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0813640	AT2G30150.1	52.321	237	100	6	77	310	147	373	3.55e-74	236
Msa0813640	AT2G30150.1	62.500	64	21	2	16	79	1	61	2.22e-17	83.2
Msa0813640	AT2G30150.2	52.321	237	100	6	77	310	163	389	4.65e-74	236
Msa0813640	AT2G30150.2	53.846	91	38	3	1	90	1	88	8.26e-21	93.6
Msa0813640	AT2G30140.1	51.261	238	99	6	77	310	164	388	7.70e-71	228
Msa0813640	AT2G30140.1	58.974	78	27	2	4	79	3	77	9.47e-21	93.2
Msa0813640	AT2G30140.2	50.840	238	99	6	77	310	164	387	1.79e-69	224
Msa0813640	AT2G30140.2	58.974	78	27	2	4	79	3	77	9.79e-21	93.2
Msa0813640	AT2G28080.1	35.417	240	134	5	80	305	179	411	2.80e-43	156
Msa0813640	AT2G36970.1	35.200	250	132	8	87	316	182	421	2.18e-42	154

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Find 102 sgRNAs with CRISPR-Local

Find 301 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGTGTGGATTACATTCC+GGG	0.361089	5_3:+44666381	Msa0813640:CDS
AAAGATATTTGTGGGGATAA+GGG	0.388826	5_3:+44666834	Msa0813640:CDS
AAAGGTTGGTCCATCATTAT+TGG	0.266008	5_3:-44666984	None:intergenic
AAAGTGGTGTTAGATTCTTG+TGG	0.695939	5_3:+44666784	Msa0813640:CDS
AACATGAAAAGCTCTGACAG+AGG	0.623253	5_3:+44667080	Msa0813640:CDS
AACGTAATTCCCTCCGAAGA+AGG	0.451880	5_3:+44663644	Msa0813640:CDS
AACGTGTGGATTACATTCCG+GGG	0.625241	5_3:+44666382	Msa0813640:CDS
AAGATATTTGTGGGGATAAG+GGG	0.481885	5_3:+44666835	Msa0813640:CDS
AATTGTTGGTGCCACCTCTT+TGG	0.300336	5_3:-44667110	None:intergenic
ACAGAGGAGGGATTCCAAAG+AGG	0.663669	5_3:+44667096	Msa0813640:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATGAAGATGTTAAGGTTGT+TGG	-	chr5_3:44663534-44663553	None:intergenic	30.0%
TGAAGGTTATGAAGATGTTA+AGG	-	chr5_3:44663541-44663560	None:intergenic	30.0%
GAATCAAGTAGTTTCTCAAA+TGG	-	chr5_3:44663726-44663745	None:intergenic	30.0%
CTATCATGTATAATCACACT+CGG	-	chr5_3:44663762-44663781	None:intergenic	30.0%
TCAAATGGCCTATAATTACA+GGG	-	chr5_3:44663814-44663833	None:intergenic	30.0%
TTCAAATGGCCTATAATTAC+AGG	-	chr5_3:44663815-44663834	None:intergenic	30.0%
ACATCAGCAAATTACCAAAA+TGG	+	chr5_3:44663926-44663945	Msa0813640:intron	30.0%
AAAAAAATTCGGGAAAAAGG+TGG	+	chr5_3:44664295-44664314	Msa0813640:intron	30.0%
AAAAAATTCGGGAAAAAGGT+GGG	+	chr5_3:44664296-44664315	Msa0813640:intron	30.0%
CTTGTAACCCAAAACAAATA+AGG	-	chr5_3:44664542-44664561	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	44663425	44667172	44663425	ID=Msa0813640;Name=Msa0813640
chr5_3	mRNA	44663425	44667172	44663425	ID=Msa0813640-mRNA-1;Parent=Msa0813640;Name=Msa0813640-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0\|0\|0\|1\|0\|1\|2\|0\|347
chr5_3	exon	44663425	44663665	44663425	ID=Msa0813640-mRNA-1:exon:13742;Parent=Msa0813640-mRNA-1
chr5_3	exon	44666370	44667172	44666370	ID=Msa0813640-mRNA-1:exon:13743;Parent=Msa0813640-mRNA-1
chr5_3	CDS	44663425	44663665	44663425	ID=Msa0813640-mRNA-1:cds;Parent=Msa0813640-mRNA-1
chr5_3	CDS	44666370	44667172	44666370	ID=Msa0813640-mRNA-1:cds;Parent=Msa0813640-mRNA-1

Gene Sequence

>Msa0813640

ATGGAACCTCCAAATCCTCCTCCACCCACCACTCACGTAGTAGCCGTACCATACCCAGGTCGCGGCCACATCAACCCAATGATGAACCTATGCAAACTCTTAATCTCCAACAACCTTAACATCTTCATAACCTTCATAGTCACAGAAGAATGGCTCACCCTCATCAACTCCGACCCTACAAAAACAAACAACAAAAACATCAAATTTGCCACCATTCCAAACGTAATTCCCTCCGAAGAAGAACTTGGTGACAAACGTGTGGATTACATTCCGGGGAACTCATCAATTCGTCTCCAAGATTTTCAACTTCACGATGCAAGCTTACGCACCCGTAGATTATTAGAATTAGCTCTGAATAACATACCATGGATGAAGAGAGCACAATATCTATTATTCCCTTCCATATATGAACTTGAGCCTCAAGCAATTGATGTTTTGAGAGAAGAATTTTCTATACCAATTTACACAATTGGCCCTACTATACCATATTTTAGTCACGGCCAAATTGCCTCTCTAAGCACCAACCAAGATGTTGAACTTGATTATATAAATTGGCTAGATAATCAACCCATTGGTTCTGTTCTATATGTCTCACAAGGGAGTTTTCTTTCGGTTTCAAGCGAACAGATCAATGAAATTGCGAATGGTTTGCGTGAAAGTGGTGTTAGATTCTTGTGGATAATGCGTGGAGAAAGTTCTAAGTGGAAAGATATTTGTGGGGATAAGGGGTTTGTTTTGCCATGGTGTGACCAATTGAGAGTGTTGATGCATAGTGCTATAGGTGGTTTTTGGTCACATTGTGGGTGGAATTCAACTAGAGAAGGTTTGTTTTGTGGTGTGCCTTTTCTTACCTCTCCAATAATGATGGACCAACCTTTGAATAGTAAGTATATAGTTGAGGATTGGAAGGTTGGGTGGAGAGTGGAGAAGGGCTTGGTTAGAAATAAAAATAACATGAAAAGCTCTGACAGAGGAGGGATTCCAAAGAGGTGGCACCAACAATTTCTGAGGAAATATAAACTCCATCAAGTGCACTCGAATTAA

Protein sequence

>Msa0813640

MEPPNPPPPTTHVVAVPYPGRGHINPMMNLCKLLISNNLNIFITFIVTEEWLTLINSDPTKTNNKNIKFATIPNVIPSEEELGDKRVDYIPGNSSIRLQDFQLHDASLRTRRLLELALNNIPWMKRAQYLLFPSIYELEPQAIDVLREEFSIPIYTIGPTIPYFSHGQIASLSTNQDVELDYINWLDNQPIGSVLYVSQGSFLSVSSEQINEIANGLRESGVRFLWIMRGESSKWKDICGDKGFVLPWCDQLRVLMHSAIGGFWSHCGWNSTREGLFCGVPFLTSPIMMDQPLNSKYIVEDWKVGWRVEKGLVRNKNNMKSSDRGGIPKRWHQQFLRKYKLHQVHSN*