AlfalfaGEDB | Msa0876530

M. sativa cultivar zhongmu-4 / Msa0876530

KEGG: K02992
GO: GO:0000028 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009579 GO:0009987 GO:0010467 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042254 GO:0042255 GO:0042274 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00177.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0876530	YP_009141653.1	95.370	108	1	1	1	104	47	154	9.29e-67	207

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0876530	sp\|Q9GE26\|RR7_AMBTC	91.667	108	5	1	1	104	47	154	2.39e-67	202

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0876530	A0A0F6NLW0	95.370	108	1	1	1	104	47	154	4.44e-67	207

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0398610	Msa0876530	0.801096	1.057918e-48	-8.615850e-47
Msa0405430	Msa0876530	0.849018	4.231674e-60	-8.615850e-47
Msa0405520	Msa0876530	0.846456	2.147216e-59	-8.615850e-47
Msa0263770	Msa0876530	0.833261	5.902903e-56	-8.615850e-47
Msa0591290	Msa0876530	0.845220	4.651490e-59	-8.615850e-47
Msa0876530	Msa1460120	0.845244	4.581729e-59	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0876530	MtrunA17_CPg0492421	85.455	110	10	2	1	104	47	156	6.54e-62	185
Msa0876530	MtrunA17_Chr4g0016101	85.455	110	10	2	1	104	47	156	6.54e-62	185
Msa0876530	MtrunA17_Chr3g0090841	85.455	110	10	2	1	104	47	156	6.54e-62	185
Msa0876530	MtrunA17_Chr4g0002541	73.684	38	10	0	1	38	23	60	3.53e-13	59.3
Msa0876530	MtrunA17_Chr4g0057401	74.286	35	9	0	70	104	5	39	7.25e-12	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0876530	ATCG00900.1	90.741	108	6	1	1	104	47	154	1.36e-67	200
Msa0876530	ATCG01240.1	90.741	108	6	1	1	104	47	154	1.36e-67	200
Msa0876530	ATMG01270.1	32.381	105	64	2	7	104	41	145	7.06e-12	58.2
Msa0876530	AT2G07696.1	32.381	105	64	2	7	104	41	145	7.06e-12	58.2

Find 21 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCCATTCGTTGGTTATT+AGG	0.268444	6_1:+14759975	Msa0876530:CDS
CATCAAGTTCCCGTTGAAAT+AGG	0.307921	6_1:+14759933	Msa0876530:CDS
AAAACGTCCAGGTCGAAATA+TGG	0.317789	6_1:+14760007	Msa0876530:CDS
TGGCGATGCCATACGCAAAA+AGG	0.366109	6_1:+14760072	Msa0876530:CDS
GCTTGACGTAAAACAGATAG+TGG	0.426676	6_1:-14759852	None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG	0.427761	6_1:+14759903	Msa0876530:CDS
TATGGCATCGCCATTCCCTT+TGG	0.436234	6_1:-14760062	None:intergenic
TGTGTGGATCCTATTTCAAC+GGG	0.470768	6_1:-14759942	None:intergenic
TTATCTATCGAGCTATGAAA+AGG	0.512086	6_1:+14759805	None:intergenic
GAAAAGCACTTGCCATTCGT+TGG	0.526859	6_1:+14759967	Msa0876530:CDS
GTGGATGCTGCCAAAGGGAA+TGG	0.532446	6_1:+14760052	Msa0876530:intron
GGCGCATCCCGAAAACGTCC+AGG	0.540732	6_1:+14759996	Msa0876530:CDS
AAAGGAAGAGACTCATAGAA+TGG	0.548096	6_1:+14760090	Msa0876530:CDS
TTGTGTGGATCCTATTTCAA+CGG	0.548545	6_1:-14759943	None:intergenic
AATTAGTGGATGCTGCCAAA+GGG	0.575951	6_1:+14760047	Msa0876530:intron
GAATTAGTGGATGCTGCCAA+AGG	0.579889	6_1:+14760046	Msa0876530:intron
AGAGACTCATAGAATGGCAG+AGG	0.593635	6_1:+14760096	Msa0876530:CDS
TTGAAAGCCATATTTCGACC+TGG	0.605724	6_1:-14760014	None:intergenic
TGCGCCTAATAACCAACGAA+TGG	0.620213	6_1:-14759979	None:intergenic
TTTACGTCAAGCAATACGTG+GGG	0.729232	6_1:+14759863	Msa0876530:CDS
GTTGAAATAGGATCCACACA+AGG	0.731975	6_1:+14759945	Msa0876530:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAAATTAAGTTCGAATTAG+TGG	+	chr6_1:14760033-14760052	Msa0876530:intron	25.0%
!!	TCTTGCTTTTACTGCTATAT+CGG	-	chr6_1:14759895-14759914	None:intergenic	30.0%
!	TTTTACGTCAAGCAATACGT+GGG	+	chr6_1:14759862-14759881	Msa0876530:CDS	35.0%
!!	CTTGCTTTTACTGCTATATC+GGG	-	chr6_1:14759894-14759913	None:intergenic	35.0%
	TTGTGTGGATCCTATTTCAA+CGG	-	chr6_1:14759946-14759965	None:intergenic	35.0%
	AAAGGAAGAGACTCATAGAA+TGG	+	chr6_1:14760090-14760109	Msa0876530:CDS	35.0%
	GCTTGACGTAAAACAGATAG+TGG	-	chr6_1:14759855-14759874	None:intergenic	40.0%
!	GTTTTACGTCAAGCAATACG+TGG	+	chr6_1:14759861-14759880	Msa0876530:CDS	40.0%
	TTTACGTCAAGCAATACGTG+GGG	+	chr6_1:14759863-14759882	Msa0876530:CDS	40.0%
	GTAAAAGCAAGACGCGTAAA+CGG	+	chr6_1:14759903-14759922	Msa0876530:CDS	40.0%
	CATCAAGTTCCCGTTGAAAT+AGG	+	chr6_1:14759933-14759952	Msa0876530:CDS	40.0%
	TGTGTGGATCCTATTTCAAC+GGG	-	chr6_1:14759945-14759964	None:intergenic	40.0%
	GTTGAAATAGGATCCACACA+AGG	+	chr6_1:14759945-14759964	Msa0876530:CDS	40.0%
!	CTTGCCATTCGTTGGTTATT+AGG	+	chr6_1:14759975-14759994	Msa0876530:CDS	40.0%
!	TATTTCGACCTGGACGTTTT+CGG	-	chr6_1:14760007-14760026	None:intergenic	40.0%
	AAAACGTCCAGGTCGAAATA+TGG	+	chr6_1:14760007-14760026	Msa0876530:CDS	40.0%
	TTGAAAGCCATATTTCGACC+TGG	-	chr6_1:14760017-14760036	None:intergenic	40.0%
!	AATTAGTGGATGCTGCCAAA+GGG	+	chr6_1:14760047-14760066	Msa0876530:intron	40.0%
!	AGTCTCTTCCTTTTTGCGTA+TGG	-	chr6_1:14760083-14760102	None:intergenic	40.0%
!!	GAAAAGCACTTGCCATTCGT+TGG	+	chr6_1:14759967-14759986	Msa0876530:CDS	45.0%
	TGCGCCTAATAACCAACGAA+TGG	-	chr6_1:14759982-14760001	None:intergenic	45.0%
!	ATTTCGACCTGGACGTTTTC+GGG	-	chr6_1:14760006-14760025	None:intergenic	45.0%
!	GAATTAGTGGATGCTGCCAA+AGG	+	chr6_1:14760046-14760065	Msa0876530:intron	45.0%
	AGAGACTCATAGAATGGCAG+AGG	+	chr6_1:14760096-14760115	Msa0876530:CDS	45.0%
!	GGCAAGTGCTTTTCCTTGTG+TGG	-	chr6_1:14759961-14759980	None:intergenic	50.0%
	TATGGCATCGCCATTCCCTT+TGG	-	chr6_1:14760065-14760084	None:intergenic	50.0%
	TGGCGATGCCATACGCAAAA+AGG	+	chr6_1:14760072-14760091	Msa0876530:CDS	50.0%
	GTGGATGCTGCCAAAGGGAA+TGG	+	chr6_1:14760052-14760071	Msa0876530:intron	55.0%
	GGCGCATCCCGAAAACGTCC+AGG	+	chr6_1:14759996-14760015	Msa0876530:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	14759819	14760141	14759819	ID=Msa0876530;Name=Msa0876530
chr6_1	mRNA	14759819	14760141	14759819	ID=Msa0876530-mRNA-1;Parent=Msa0876530;Name=Msa0876530-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0\|0\|0\|1\|0\|0\|2\|0\|104
chr6_1	exon	14759819	14760041	14759819	ID=Msa0876530-mRNA-1:exon:4235;Parent=Msa0876530-mRNA-1
chr6_1	exon	14760053	14760141	14760053	ID=Msa0876530-mRNA-1:exon:4236;Parent=Msa0876530-mRNA-1
chr6_1	CDS	14759819	14760041	14759819	ID=Msa0876530-mRNA-1:cds;Parent=Msa0876530-mRNA-1
chr6_1	CDS	14760053	14760141	14760053	ID=Msa0876530-mRNA-1:cds;Parent=Msa0876530-mRNA-1

Gene Sequence	>Msa0876530 ATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCTGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAATGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTT
Protein sequence	>Msa0876530 MKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLMDAAKGNGDAIRKKEETHRMAEANRAFAHF