AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0398610


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0398610 MtrunA17_CPg0492781 98.333 60 1 0 46 105 7 66 1.19e-38 124
Msa0398610 MtrunA17_Chr4g0015791 98.333 60 1 0 46 105 7 66 1.19e-38 124
Msa0398610 MtrunA17_Chr8g0355601 96.610 59 2 0 46 104 7 65 1.85e-37 120
Msa0398610 MtrunA17_Chr7g0224991 95.455 44 2 0 62 105 6 49 8.03e-26 90.9
Msa0398610 MtrunA17_CPg0492791 93.750 32 2 0 15 46 1 32 2.16e-15 64.3
Msa0398610 MtrunA17_Chr4g0015781 93.750 32 2 0 15 46 1 32 2.16e-15 64.3
Msa0398610 MtrunA17_Chr4g0028951 93.750 32 2 0 15 46 1 32 2.16e-15 64.3
Msa0398610 MtrunA17_Chr8g0355611 93.750 32 2 0 15 46 1 32 2.16e-15 64.3
Msa0398610 MtrunA17_Chr4g0016881 96.774 31 1 0 15 45 1 31 6.36e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0398610 ATCG00640.1 75.000 60 15 0 46 105 7 66 2.54e-29 100
Msa0398610 ATCG00630.1 84.375 32 5 0 15 46 1 32 9.35e-13 58.2

Find 13 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACGAATAACTGTTATTT+TGG 0.125276 3_2:+20507728 Msa0398610:CDS
AGAGTGTTAATAAGGAATCT+AGG 0.329597 3_2:+20507772 Msa0398610:CDS
AATCGTATGTTTGCAACAAA+AGG 0.396841 3_2:-20507868 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 3_2:+20507104 Msa0398610:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 3_2:+20507773 Msa0398610:CDS
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 3_2:+20507121 Msa0398610:CDS
ACACAATACACCTAGTCGAT+TGG 0.524208 3_2:+20507827 Msa0398610:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 3_2:+20507111 Msa0398610:CDS
ATCGTATGTTTGCAACAAAA+GGG 0.538893 3_2:-20507867 None:intergenic
TTTCTCAATTCCAATCGACT+AGG 0.574270 3_2:-20507837 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 3_2:+20507090 Msa0398610:CDS
AACCATAGAGTACTTGCCAC+TGG 0.586013 3_2:-20507106 None:intergenic
AGTGTTAATAAGGAATCTAG+GGG 0.667965 3_2:+20507774 Msa0398610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTAGAATCAAAAAAA+GGG - chr3_2:20507193-20507212 None:intergenic 10.0%
!! AATAATTAGAATCAAAAAAA+GGG - chr3_2:20507193-20507212 None:intergenic 10.0%
!! ATAATTAGAATCAAAAAAAG+GGG - chr3_2:20507192-20507211 None:intergenic 15.0%
!! CAATAATTAGAATCAAAAAA+AGG - chr3_2:20507194-20507213 None:intergenic 15.0%
!!! CTTTTTTTGATTCTAATTAT+TGG + chr3_2:20507192-20507211 Msa0398610:intron 15.0%
!! AGAGCAAATTAATTTAATAA+AGG + chr3_2:20507314-20507333 Msa0398610:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG - chr3_2:20507408-20507427 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG + chr3_2:20507564-20507583 Msa0398610:intron 15.0%
!! ATAATTAGAATCAAAAAAAG+GGG - chr3_2:20507192-20507211 None:intergenic 15.0%
!! CAATAATTAGAATCAAAAAA+AGG - chr3_2:20507194-20507213 None:intergenic 15.0%
!!! CTTTTTTTGATTCTAATTAT+TGG + chr3_2:20507192-20507211 Msa0398610:intron 15.0%
!! AGAGCAAATTAATTTAATAA+AGG + chr3_2:20507314-20507333 Msa0398610:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG - chr3_2:20507408-20507427 None:intergenic 15.0%
!! CTGTACTATATAAAATTAAT+TGG + chr3_2:20507564-20507583 Msa0398610:intron 15.0%
!! AAATTACAAGAAAAAAACGA+AGG + chr3_2:20507040-20507059 Msa0398610:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG - chr3_2:20507247-20507266 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG + chr3_2:20507317-20507336 Msa0398610:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG + chr3_2:20507323-20507342 Msa0398610:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG - chr3_2:20507439-20507458 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG + chr3_2:20507728-20507747 Msa0398610:CDS 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr3_2:20507764-20507783 Msa0398610:CDS 20.0%
!! AAATTACAAGAAAAAAACGA+AGG + chr3_2:20507040-20507059 Msa0398610:CDS 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG - chr3_2:20507247-20507266 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG + chr3_2:20507317-20507336 Msa0398610:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG + chr3_2:20507323-20507342 Msa0398610:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG - chr3_2:20507439-20507458 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG + chr3_2:20507728-20507747 Msa0398610:CDS 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr3_2:20507764-20507783 Msa0398610:CDS 20.0%
! TTTGATTCTAATTATTGGCA+CGG + chr3_2:20507197-20507216 Msa0398610:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG - chr3_2:20507264-20507283 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG + chr3_2:20507443-20507462 Msa0398610:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG + chr3_2:20507444-20507463 Msa0398610:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG + chr3_2:20507682-20507701 Msa0398610:intron 25.0%
! TTTGATTCTAATTATTGGCA+CGG + chr3_2:20507197-20507216 Msa0398610:intron 25.0%
! AAAAAAAAAACGAACGAAGA+GGG - chr3_2:20507264-20507283 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG + chr3_2:20507443-20507462 Msa0398610:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG + chr3_2:20507444-20507463 Msa0398610:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG + chr3_2:20507682-20507701 Msa0398610:intron 25.0%
TTACAAGAAAAAAACGAAGG+AGG + chr3_2:20507043-20507062 Msa0398610:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG + chr3_2:20507081-20507100 Msa0398610:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG + chr3_2:20507153-20507172 Msa0398610:intron 30.0%
GAAAAAAAAAACGAACGAAG+AGG - chr3_2:20507265-20507284 None:intergenic 30.0%
AAAAACTCCTAATTTCACTG+AGG - chr3_2:20507349-20507368 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG + chr3_2:20507423-20507442 Msa0398610:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG + chr3_2:20507447-20507466 Msa0398610:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG + chr3_2:20507685-20507704 Msa0398610:intron 30.0%
TATTCGTATATCTTTACCCT+TGG - chr3_2:20507718-20507737 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr3_2:20507772-20507791 Msa0398610:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr3_2:20507773-20507792 Msa0398610:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr3_2:20507774-20507793 Msa0398610:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr3_2:20507870-20507889 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr3_2:20507871-20507890 None:intergenic 30.0%
TTACAAGAAAAAAACGAAGG+AGG + chr3_2:20507043-20507062 Msa0398610:CDS 30.0%
TCTAAAAACATATCTCTCTG+TGG + chr3_2:20507081-20507100 Msa0398610:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG + chr3_2:20507153-20507172 Msa0398610:intron 30.0%
GAAAAAAAAAACGAACGAAG+AGG - chr3_2:20507265-20507284 None:intergenic 30.0%
AAAAACTCCTAATTTCACTG+AGG - chr3_2:20507349-20507368 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG + chr3_2:20507423-20507442 Msa0398610:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG + chr3_2:20507447-20507466 Msa0398610:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG + chr3_2:20507685-20507704 Msa0398610:intron 30.0%
TATTCGTATATCTTTACCCT+TGG - chr3_2:20507718-20507737 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr3_2:20507772-20507791 Msa0398610:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr3_2:20507773-20507792 Msa0398610:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr3_2:20507774-20507793 Msa0398610:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr3_2:20507870-20507889 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr3_2:20507871-20507890 None:intergenic 30.0%
! TGAAATTAGGAGTTTTTCCC+AGG + chr3_2:20507352-20507371 Msa0398610:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG - chr3_2:20507372-20507391 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG - chr3_2:20507373-20507392 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG + chr3_2:20507448-20507467 Msa0398610:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG - chr3_2:20507643-20507662 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG + chr3_2:20507692-20507711 Msa0398610:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG - chr3_2:20507840-20507859 None:intergenic 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG + chr3_2:20507352-20507371 Msa0398610:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG - chr3_2:20507372-20507391 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG - chr3_2:20507373-20507392 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG + chr3_2:20507448-20507467 Msa0398610:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG - chr3_2:20507643-20507662 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG + chr3_2:20507692-20507711 Msa0398610:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG - chr3_2:20507840-20507859 None:intergenic 35.0%
TTCTAATTATTGGCACGGCA+AGG + chr3_2:20507202-20507221 Msa0398610:intron 40.0%
TCTAATTATTGGCACGGCAA+GGG + chr3_2:20507203-20507222 Msa0398610:intron 40.0%
GATTTGGCCTCAGTGAAATT+AGG + chr3_2:20507339-20507358 Msa0398610:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG + chr3_2:20507361-20507380 Msa0398610:intron 40.0%
ACACAATACACCTAGTCGAT+TGG + chr3_2:20507827-20507846 Msa0398610:CDS 40.0%
TTCTAATTATTGGCACGGCA+AGG + chr3_2:20507202-20507221 Msa0398610:intron 40.0%
TCTAATTATTGGCACGGCAA+GGG + chr3_2:20507203-20507222 Msa0398610:intron 40.0%
GATTTGGCCTCAGTGAAATT+AGG + chr3_2:20507339-20507358 Msa0398610:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG + chr3_2:20507361-20507380 Msa0398610:intron 40.0%
ACACAATACACCTAGTCGAT+TGG + chr3_2:20507827-20507846 Msa0398610:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG - chr3_2:20507109-20507128 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG - chr3_2:20507175-20507194 None:intergenic 45.0%
CTAATTATTGGCACGGCAAG+GGG + chr3_2:20507204-20507223 Msa0398610:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG - chr3_2:20507109-20507128 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG - chr3_2:20507175-20507194 None:intergenic 45.0%
CTAATTATTGGCACGGCAAG+GGG + chr3_2:20507204-20507223 Msa0398610:intron 45.0%
ATATCTCTCTGTGGCACCAG+TGG + chr3_2:20507090-20507109 Msa0398610:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG + chr3_2:20507104-20507123 Msa0398610:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG + chr3_2:20507111-20507130 Msa0398610:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG - chr3_2:20507174-20507193 None:intergenic 50.0%
CAAAGGAGGTTCATGGCCAA+GGG + chr3_2:20507699-20507718 Msa0398610:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG + chr3_2:20507090-20507109 Msa0398610:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG + chr3_2:20507104-20507123 Msa0398610:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG + chr3_2:20507111-20507130 Msa0398610:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG - chr3_2:20507174-20507193 None:intergenic 50.0%
CAAAGGAGGTTCATGGCCAA+GGG + chr3_2:20507699-20507718 Msa0398610:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG + chr3_2:20507121-20507140 Msa0398610:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG - chr3_2:20507701-20507720 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG + chr3_2:20507698-20507717 Msa0398610:intron 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG + chr3_2:20507121-20507140 Msa0398610:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG - chr3_2:20507701-20507720 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG + chr3_2:20507698-20507717 Msa0398610:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 20507031 20507911 20507031 ID=Msa0398610;Name=Msa0398610
chr3_2 mRNA 20507031 20507911 20507031 ID=Msa0398610-mRNA-1;Parent=Msa0398610;Name=Msa0398610-mRNA-1;_AED=0.39;_eAED=0.40;_QI=0|0|0|1|0|0|2|0|105
chr3_2 exon 20507031 20507163 20507031 ID=Msa0398610-mRNA-1:exon:4473;Parent=Msa0398610-mRNA-1
chr3_2 exon 20507727 20507911 20507727 ID=Msa0398610-mRNA-1:exon:4474;Parent=Msa0398610-mRNA-1
chr3_2 CDS 20507031 20507163 20507031 ID=Msa0398610-mRNA-1:cds;Parent=Msa0398610-mRNA-1
chr3_2 CDS 20507727 20507911 20507727 ID=Msa0398610-mRNA-1:cds;Parent=Msa0398610-mRNA-1
Gene Sequence

>Msa0398610

ATGTATTACAAATTACAAGAAAAAAACGAAGGAGGATTTGAAATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCATATACGAATAACTGTTATTTTGGAATGTAGCAGTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGGTATTTCTAGATATATTACTCAAAAGAATCGACACAATACACCTAGTCGATTGGAATTGAGAAAATTCTGTCCCTTTTGTTGCAAACATACGATTCACGTCGAGATAAAGAAATAG

Protein sequence

>Msa0398610

MYYKLQEKNEGGFEMRDLKTYLSVAPVASTLWFAVLAGLLIEINHIRITVILECSSCDKKSVNKESRGISRYITQKNRHNTPSRLELRKFCPFCCKHTIHVEIKK*