Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0452900 | Msa0899600 | 0.800427 | 1.448576e-48 | -8.615850e-47 |
| Msa0454910 | Msa0899600 | 0.804343 | 2.260761e-49 | -8.615850e-47 |
| Msa0502760 | Msa0899600 | 0.805676 | 1.189755e-49 | -8.615850e-47 |
| Msa0598780 | Msa0899600 | 0.800225 | 1.592356e-48 | -8.615850e-47 |
| Msa0846210 | Msa0899600 | 0.838602 | 2.608670e-57 | -8.615850e-47 |
| Msa0899600 | Msa0979410 | 0.823999 | 1.023318e-53 | -8.615850e-47 |
| Msa0899600 | Msa1025370 | 0.804628 | 1.971970e-49 | -8.615850e-47 |
| Msa0899600 | Msa1044830 | 0.807563 | 4.754960e-50 | -8.615850e-47 |
| Msa0899600 | Msa1044840 | 0.802382 | 5.762164e-49 | -8.615850e-47 |
| Msa0899600 | Msa1100980 | 0.825010 | 5.917091e-54 | -8.615850e-47 |
| Msa0899600 | Msa1135740 | 0.809219 | 2.108105e-50 | -8.615850e-47 |
| Msa0899600 | Msa1187950 | 0.802523 | 5.388365e-49 | -8.615850e-47 |
| Msa0899600 | Msa1283330 | 0.801593 | 8.367070e-49 | -8.615850e-47 |
| Msa0899600 | Msa1422180 | 0.806242 | 9.045148e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGACAAATCAACAATTGC+TGG | 0.319414 | 6_1:+94843743 | Msa0899600:CDS |
| TGATATCATCAGGGTGAGTT+TGG | 0.349530 | 6_1:+94843370 | Msa0899600:intron |
| TAAGTTCTAATAACTTCCTT+AGG | 0.383205 | 6_1:-94843800 | None:intergenic |
| GAACTATGTTTATAGGAACT+TGG | 0.458558 | 6_1:+94843130 | Msa0899600:intron |
| CAATGGATTGTGAAGACGTC+CGG | 0.496950 | 6_1:-94843200 | None:intergenic |
| TGCTTCATAGTTTAGATGGA+AGG | 0.499677 | 6_1:+94843019 | None:intergenic |
| ATATTACCTAGTTGCAGCAA+TGG | 0.510361 | 6_1:-94843217 | None:intergenic |
| ATAACTTCCTTAGGTCTTAG+CGG | 0.539854 | 6_1:-94843791 | None:intergenic |
| ACAGGAGCTGAAGTAGAGAA+AGG | 0.563721 | 6_1:+94843422 | Msa0899600:CDS |
| TTCAGCACCGCTAAGACCTA+AGG | 0.564308 | 6_1:+94843784 | Msa0899600:CDS |
| GAAGAAGCTTAATCAAAGAC+CGG | 0.572062 | 6_1:+94843181 | Msa0899600:CDS |
| CACAATCCATTGCTGCAACT+AGG | 0.574804 | 6_1:+94843211 | Msa0899600:CDS |
| TCAACAATTGCTGGTGTACG+TGG | 0.590971 | 6_1:+94843752 | Msa0899600:CDS |
| ATTTGTCTAAGAAATATCCA+AGG | 0.624404 | 6_1:-94843730 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTTGAACTATGTTTAT+AGG | + | chr6_1:94843123-94843142 | Msa0899600:intron | 15.0% |
| !! | GTAAAATTGAAAAAATAAGA+TGG | - | chr6_1:94843278-94843297 | None:intergenic | 15.0% |
| !!! | TAATTTTGAACTATGTTTAT+AGG | + | chr6_1:94843123-94843142 | Msa0899600:intron | 15.0% |
| !! | GTAAAATTGAAAAAATAAGA+TGG | - | chr6_1:94843278-94843297 | None:intergenic | 15.0% |
| !! | CTGAAATTAAAGTTCAAAAA+GGG | + | chr6_1:94843344-94843363 | Msa0899600:intron | 20.0% |
| !!! | TGAATGATGGTTTAAAATAA+TGG | + | chr6_1:94843554-94843573 | Msa0899600:intron | 20.0% |
| !!! | TTAAAATAATGGTTTGTCTA+TGG | + | chr6_1:94843565-94843584 | Msa0899600:intron | 20.0% |
| !!! | AATTTTCTGATGTATAATGA+GGG | + | chr6_1:94843629-94843648 | Msa0899600:intron | 20.0% |
| !! | CTGAAATTAAAGTTCAAAAA+GGG | + | chr6_1:94843344-94843363 | Msa0899600:intron | 20.0% |
| !!! | TGAATGATGGTTTAAAATAA+TGG | + | chr6_1:94843554-94843573 | Msa0899600:intron | 20.0% |
| !!! | TTAAAATAATGGTTTGTCTA+TGG | + | chr6_1:94843565-94843584 | Msa0899600:intron | 20.0% |
| !!! | AATTTTCTGATGTATAATGA+GGG | + | chr6_1:94843629-94843648 | Msa0899600:intron | 20.0% |
| ! | GCTGAAATTAAAGTTCAAAA+AGG | + | chr6_1:94843343-94843362 | Msa0899600:intron | 25.0% |
| !!! | CAATTTTCTAGTTGTTCTTT+TGG | + | chr6_1:94843397-94843416 | Msa0899600:CDS | 25.0% |
| ! | AGAAGTTAATGACCAAATTA+AGG | + | chr6_1:94843470-94843489 | Msa0899600:intron | 25.0% |
| ! | GAAGTTAATGACCAAATTAA+GGG | + | chr6_1:94843471-94843490 | Msa0899600:intron | 25.0% |
| !!! | TAGATTTCTAAAGCATGTAA+AGG | - | chr6_1:94843603-94843622 | None:intergenic | 25.0% |
| !! | GAATTTTCTGATGTATAATG+AGG | + | chr6_1:94843628-94843647 | Msa0899600:intron | 25.0% |
| ! | TGATTCTGTACAAAAATACA+AGG | - | chr6_1:94843708-94843727 | None:intergenic | 25.0% |
| ! | ATTTGTCTAAGAAATATCCA+AGG | - | chr6_1:94843733-94843752 | None:intergenic | 25.0% |
| !! | TAAGTTCTAATAACTTCCTT+AGG | - | chr6_1:94843803-94843822 | None:intergenic | 25.0% |
| ! | GCTGAAATTAAAGTTCAAAA+AGG | + | chr6_1:94843343-94843362 | Msa0899600:intron | 25.0% |
| !!! | CAATTTTCTAGTTGTTCTTT+TGG | + | chr6_1:94843397-94843416 | Msa0899600:CDS | 25.0% |
| ! | AGAAGTTAATGACCAAATTA+AGG | + | chr6_1:94843470-94843489 | Msa0899600:intron | 25.0% |
| ! | GAAGTTAATGACCAAATTAA+GGG | + | chr6_1:94843471-94843490 | Msa0899600:intron | 25.0% |
| !!! | TAGATTTCTAAAGCATGTAA+AGG | - | chr6_1:94843603-94843622 | None:intergenic | 25.0% |
| !! | GAATTTTCTGATGTATAATG+AGG | + | chr6_1:94843628-94843647 | Msa0899600:intron | 25.0% |
| ! | TGATTCTGTACAAAAATACA+AGG | - | chr6_1:94843708-94843727 | None:intergenic | 25.0% |
| ! | ATTTGTCTAAGAAATATCCA+AGG | - | chr6_1:94843733-94843752 | None:intergenic | 25.0% |
| !! | TAAGTTCTAATAACTTCCTT+AGG | - | chr6_1:94843803-94843822 | None:intergenic | 25.0% |
| GAACTATGTTTATAGGAACT+TGG | + | chr6_1:94843130-94843149 | Msa0899600:intron | 30.0% | |
| AATGACCAAATTAAGGGTAA+CGG | + | chr6_1:94843477-94843496 | Msa0899600:intron | 30.0% | |
| !! | TTTCTAAAGCATGTAAAGGA+GGG | - | chr6_1:94843599-94843618 | None:intergenic | 30.0% |
| !! | ATTTCTAAAGCATGTAAAGG+AGG | - | chr6_1:94843600-94843619 | None:intergenic | 30.0% |
| ! | TTTTGTACAGAATCAAACCT+TGG | + | chr6_1:94843713-94843732 | Msa0899600:intron | 30.0% |
| TTAGACAAATCAACAATTGC+TGG | + | chr6_1:94843743-94843762 | Msa0899600:CDS | 30.0% | |
| GAACTATGTTTATAGGAACT+TGG | + | chr6_1:94843130-94843149 | Msa0899600:intron | 30.0% | |
| AATGACCAAATTAAGGGTAA+CGG | + | chr6_1:94843477-94843496 | Msa0899600:intron | 30.0% | |
| !! | TTTCTAAAGCATGTAAAGGA+GGG | - | chr6_1:94843599-94843618 | None:intergenic | 30.0% |
| !! | ATTTCTAAAGCATGTAAAGG+AGG | - | chr6_1:94843600-94843619 | None:intergenic | 30.0% |
| ! | TTTTGTACAGAATCAAACCT+TGG | + | chr6_1:94843713-94843732 | Msa0899600:intron | 30.0% |
| TTAGACAAATCAACAATTGC+TGG | + | chr6_1:94843743-94843762 | Msa0899600:CDS | 30.0% | |
| TCCTTCGTTACATGATCAAA+TGG | + | chr6_1:94843071-94843090 | Msa0899600:intron | 35.0% | |
| ACCATTTGATCATGTAACGA+AGG | - | chr6_1:94843075-94843094 | None:intergenic | 35.0% | |
| GAAGAAGCTTAATCAAAGAC+CGG | + | chr6_1:94843181-94843200 | Msa0899600:CDS | 35.0% | |
| ATATTACCTAGTTGCAGCAA+TGG | - | chr6_1:94843220-94843239 | None:intergenic | 35.0% | |
| TGCTGCAACTAGGTAATATT+TGG | + | chr6_1:94843221-94843240 | Msa0899600:intron | 35.0% | |
| TGCAACTAGGTAATATTTGG+TGG | + | chr6_1:94843224-94843243 | Msa0899600:intron | 35.0% | |
| ! | AACTAGGTAATATTTGGTGG+TGG | + | chr6_1:94843227-94843246 | Msa0899600:intron | 35.0% |
| AAAAGGGCTATGATATCATC+AGG | + | chr6_1:94843360-94843379 | Msa0899600:intron | 35.0% | |
| AAAGGGCTATGATATCATCA+GGG | + | chr6_1:94843361-94843380 | Msa0899600:intron | 35.0% | |
| TAAAGCCGTTACCCTTAATT+TGG | - | chr6_1:94843485-94843504 | None:intergenic | 35.0% | |
| CATAAATATGTGTACCGCAA+AGG | - | chr6_1:94843674-94843693 | None:intergenic | 35.0% | |
| ATAACTTCCTTAGGTCTTAG+CGG | - | chr6_1:94843794-94843813 | None:intergenic | 35.0% | |
| TCCTTCGTTACATGATCAAA+TGG | + | chr6_1:94843071-94843090 | Msa0899600:intron | 35.0% | |
| ACCATTTGATCATGTAACGA+AGG | - | chr6_1:94843075-94843094 | None:intergenic | 35.0% | |
| GAAGAAGCTTAATCAAAGAC+CGG | + | chr6_1:94843181-94843200 | Msa0899600:CDS | 35.0% | |
| ATATTACCTAGTTGCAGCAA+TGG | - | chr6_1:94843220-94843239 | None:intergenic | 35.0% | |
| TGCTGCAACTAGGTAATATT+TGG | + | chr6_1:94843221-94843240 | Msa0899600:intron | 35.0% | |
| TGCAACTAGGTAATATTTGG+TGG | + | chr6_1:94843224-94843243 | Msa0899600:intron | 35.0% | |
| ! | AACTAGGTAATATTTGGTGG+TGG | + | chr6_1:94843227-94843246 | Msa0899600:intron | 35.0% |
| AAAAGGGCTATGATATCATC+AGG | + | chr6_1:94843360-94843379 | Msa0899600:intron | 35.0% | |
| AAAGGGCTATGATATCATCA+GGG | + | chr6_1:94843361-94843380 | Msa0899600:intron | 35.0% | |
| TAAAGCCGTTACCCTTAATT+TGG | - | chr6_1:94843485-94843504 | None:intergenic | 35.0% | |
| CATAAATATGTGTACCGCAA+AGG | - | chr6_1:94843674-94843693 | None:intergenic | 35.0% | |
| ATAACTTCCTTAGGTCTTAG+CGG | - | chr6_1:94843794-94843813 | None:intergenic | 35.0% | |
| TGATATCATCAGGGTGAGTT+TGG | + | chr6_1:94843370-94843389 | Msa0899600:intron | 40.0% | |
| !! | CTAGTTGTTCTTTTGGTGAC+AGG | + | chr6_1:94843404-94843423 | Msa0899600:CDS | 40.0% |
| ATTCAAAGCAGATGCGTTCA+CGG | - | chr6_1:94843539-94843558 | None:intergenic | 40.0% | |
| ! | AACGCATCTGCTTTGAATGA+TGG | + | chr6_1:94843541-94843560 | Msa0899600:intron | 40.0% |
| CTCTTCTATCATCTCCTTTG+CGG | + | chr6_1:94843657-94843676 | Msa0899600:intron | 40.0% | |
| TGATATCATCAGGGTGAGTT+TGG | + | chr6_1:94843370-94843389 | Msa0899600:intron | 40.0% | |
| !! | CTAGTTGTTCTTTTGGTGAC+AGG | + | chr6_1:94843404-94843423 | Msa0899600:CDS | 40.0% |
| ATTCAAAGCAGATGCGTTCA+CGG | - | chr6_1:94843539-94843558 | None:intergenic | 40.0% | |
| ! | AACGCATCTGCTTTGAATGA+TGG | + | chr6_1:94843541-94843560 | Msa0899600:intron | 40.0% |
| CTCTTCTATCATCTCCTTTG+CGG | + | chr6_1:94843657-94843676 | Msa0899600:intron | 40.0% | |
| !! | CAATGGATTGTGAAGACGTC+CGG | - | chr6_1:94843203-94843222 | None:intergenic | 45.0% |
| CACAATCCATTGCTGCAACT+AGG | + | chr6_1:94843211-94843230 | Msa0899600:CDS | 45.0% | |
| ACAGGAGCTGAAGTAGAGAA+AGG | + | chr6_1:94843422-94843441 | Msa0899600:CDS | 45.0% | |
| TCAACAATTGCTGGTGTACG+TGG | + | chr6_1:94843752-94843771 | Msa0899600:CDS | 45.0% | |
| !! | CAATGGATTGTGAAGACGTC+CGG | - | chr6_1:94843203-94843222 | None:intergenic | 45.0% |
| CACAATCCATTGCTGCAACT+AGG | + | chr6_1:94843211-94843230 | Msa0899600:CDS | 45.0% | |
| ACAGGAGCTGAAGTAGAGAA+AGG | + | chr6_1:94843422-94843441 | Msa0899600:CDS | 45.0% | |
| TCAACAATTGCTGGTGTACG+TGG | + | chr6_1:94843752-94843771 | Msa0899600:CDS | 45.0% | |
| GCAGATGCGTTCACGGTTAA+TGG | - | chr6_1:94843532-94843551 | None:intergenic | 50.0% | |
| TTCAGCACCGCTAAGACCTA+AGG | + | chr6_1:94843784-94843803 | Msa0899600:CDS | 50.0% | |
| GCAGATGCGTTCACGGTTAA+TGG | - | chr6_1:94843532-94843551 | None:intergenic | 50.0% | |
| TTCAGCACCGCTAAGACCTA+AGG | + | chr6_1:94843784-94843803 | Msa0899600:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 94843034 | 94843823 | 94843034 | ID=Msa0899600;Name=Msa0899600 |
| chr6_1 | mRNA | 94843034 | 94843823 | 94843034 | ID=Msa0899600-mRNA-1;Parent=Msa0899600;Name=Msa0899600-mRNA-1;_AED=0.28;_eAED=0.62;_QI=0|0|0|0.5|1|1|4|0|95 |
| chr6_1 | exon | 94843034 | 94843040 | 94843034 | ID=Msa0899600-mRNA-1:exon:16251;Parent=Msa0899600-mRNA-1 |
| chr6_1 | exon | 94843145 | 94843232 | 94843145 | ID=Msa0899600-mRNA-1:exon:16252;Parent=Msa0899600-mRNA-1 |
| chr6_1 | exon | 94843382 | 94843473 | 94843382 | ID=Msa0899600-mRNA-1:exon:16253;Parent=Msa0899600-mRNA-1 |
| chr6_1 | exon | 94843723 | 94843823 | 94843723 | ID=Msa0899600-mRNA-1:exon:16254;Parent=Msa0899600-mRNA-1 |
| chr6_1 | CDS | 94843034 | 94843040 | 94843034 | ID=Msa0899600-mRNA-1:cds;Parent=Msa0899600-mRNA-1 |
| chr6_1 | CDS | 94843145 | 94843232 | 94843145 | ID=Msa0899600-mRNA-1:cds;Parent=Msa0899600-mRNA-1 |
| chr6_1 | CDS | 94843382 | 94843473 | 94843382 | ID=Msa0899600-mRNA-1:cds;Parent=Msa0899600-mRNA-1 |
| chr6_1 | CDS | 94843723 | 94843823 | 94843723 | ID=Msa0899600-mRNA-1:cds;Parent=Msa0899600-mRNA-1 |
| Gene Sequence |
| Protein sequence |
