AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0905980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0905980 MtrunA17_Chr6g0468681 51.765 85 38 2 32 116 18 99 1.47e-20 85.1
Msa0905980 MtrunA17_Chr5g0430671 25.806 248 156 8 32 267 1 232 5.12e-12 65.9
Msa0905980 MtrunA17_Chr4g0006871 30.952 126 83 2 147 268 105 230 1.84e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 62 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAAGAGAGATTTGATTTA+TGG 0.125921 6_2:-4443606 Msa0905980:CDS
TCGTCATTGGAACATGTTTC+TGG 0.158342 6_2:+4443784 None:intergenic
AAATCGCCAAACATCATTAA+AGG 0.271490 6_2:+4443547 None:intergenic
GGAATTATTATGAGGATTGT+AGG 0.335929 6_2:-4443979 Msa0905980:CDS
TCAACACCTTTAATGATGTT+TGG 0.344659 6_2:-4443553 Msa0905980:CDS
AGGATTGTAGGTTGGAATAT+AGG 0.355761 6_2:-4443967 Msa0905980:CDS
TATTGACGTGTCTTTCCTAT+TGG 0.374502 6_2:-4443647 Msa0905980:CDS
AGAAATGGTGGAGCGTTCTC+AGG 0.377089 6_2:-4447883 Msa0905980:CDS
TTGACCAAGCCCAACACAAT+TGG 0.391633 6_2:+4443886 None:intergenic
CGCCCTCTATTAGTTCAATC+TGG 0.398255 6_2:-4443277 Msa0905980:CDS
AGAAGGAGGACAATGTAAAC+TGG 0.407921 6_2:-4443816 Msa0905980:CDS
AACATATAAGAGATACATCT+TGG 0.410574 6_2:-4443695 Msa0905980:CDS
TCATTACCGATTATTTCTTC+CGG 0.412377 6_2:+4443508 None:intergenic
GACGGGGATTGGTATTCTCT+TGG 0.416245 6_2:-4443750 Msa0905980:CDS
TCCAAGGGTTTGGCAAGAAA+TGG 0.416459 6_2:-4447898 Msa0905980:CDS
CCTTGTGGAGCTTCCGTTGT+TGG 0.424252 6_2:-4443434 Msa0905980:CDS
ACCATTTCTTGCCAAACCCT+TGG 0.440889 6_2:+4447897 None:intergenic
TTGGAACCGGAAGAAATAAT+CGG 0.443270 6_2:-4443514 Msa0905980:CDS
GAACCAGATTGAACTAATAG+AGG 0.449982 6_2:+4443274 None:intergenic
TTATTATGAGGATTGTAGGT+TGG 0.450140 6_2:-4443975 Msa0905980:CDS
GTTGACATTACAAGCTTATG+TGG 0.450630 6_2:-4443247 Msa0905980:CDS
CTCTATCTCTTCTGCTTGCA+CGG 0.450899 6_2:+4443377 None:intergenic
TAATCCAATTGTGTTGGGCT+TGG 0.460396 6_2:-4443890 Msa0905980:CDS
TGTGCAGAGGAATTATTATG+AGG 0.467824 6_2:-4443987 Msa0905980:intron
ATGAGAAAAGTAAGGATAAG+TGG 0.474209 6_2:+4447934 None:intergenic
AGGTGTAATCCAATTGTGTT+GGG 0.478595 6_2:-4443895 Msa0905980:CDS
AACTCGGTGCATGAACCAAT+AGG 0.486014 6_2:+4443632 None:intergenic
AAGAGATACATCTTGGTTGA+AGG 0.504413 6_2:-4443688 Msa0905980:CDS
GAAGGAAAATTGATTCAAGA+AGG 0.505714 6_2:-4443670 Msa0905980:CDS
TAGGTGTAATCCAATTGTGT+TGG 0.514511 6_2:-4443896 Msa0905980:CDS
GGAATGGCTAGAGCATTTCA+AGG 0.514887 6_2:-4443341 Msa0905980:CDS
AGTTAAAGGATGGTGGAAGA+AGG 0.521573 6_2:-4443833 Msa0905980:CDS
ATTGTAGGTTGGAATATAGG+AGG 0.525683 6_2:-4443964 Msa0905980:CDS
TTGTAGGTTGGAATATAGGA+GGG 0.531381 6_2:-4443963 Msa0905980:CDS
ATAGGAGGGATTACTCAGTC+AGG 0.542546 6_2:-4443949 Msa0905980:CDS
TGTTCCAATGACGAGAAAAG+TGG 0.545436 6_2:-4443775 Msa0905980:CDS
GTTCCAATGACGAGAAAAGT+GGG 0.548017 6_2:-4443774 Msa0905980:CDS
CTTATGTGGAAACTCAGACT+TGG 0.550971 6_2:-4443233 Msa0905980:CDS
TAAAGGATGGTGGAAGAAGG+AGG 0.551329 6_2:-4443830 Msa0905980:CDS
GGAAGAAATAATCGGTAATG+AGG 0.557753 6_2:-4443506 Msa0905980:CDS
GGTAATGAGGTGACTTCACA+AGG 0.569428 6_2:-4443493 Msa0905980:CDS
AAGGGTTTGGCAAGAAATGG+TGG 0.570224 6_2:-4447895 Msa0905980:CDS
ATCGTGGAACTTAGAAGTGA+AGG 0.577239 6_2:-4443152 Msa0905980:three_prime_UTR
TCATTTCAGCTATACAATGT+CGG 0.588108 6_2:+4443458 None:intergenic
CATGATAACAATTGGTATCG+TGG 0.594148 6_2:-4443168 Msa0905980:exon
ATATCCGTGATGTTGTTCAT+AGG 0.599575 6_2:-4443915 Msa0905980:CDS
GAAATGGTGGAGCGTTCTCA+GGG 0.605086 6_2:-4447882 Msa0905980:intron
AATCTCTCTTGACTGCAACT+CGG 0.607370 6_2:+4443616 None:intergenic
TGAAGCTGCGGGGATTAGAG+AGG 0.608404 6_2:-4443315 Msa0905980:CDS
AAACTCAGACTTGGACGTGA+AGG 0.611935 6_2:-4443224 Msa0905980:CDS
TACTCAGTCAGGAAAATCTG+TGG 0.624379 6_2:-4443938 Msa0905980:CDS
CAGACTTGGACGTGAAGGAG+AGG 0.636238 6_2:-4443219 Msa0905980:CDS
AACCAGATTGAACTAATAGA+GGG 0.638427 6_2:+4443275 None:intergenic
GAAGGAGGACAATGTAAACT+GGG 0.639227 6_2:-4443815 Msa0905980:CDS
TACACCTATGAACAACATCA+CGG 0.643677 6_2:+4443911 None:intergenic
CACGCAACATTCGAGTCTCA+CGG 0.650462 6_2:-4443863 Msa0905980:CDS
GGAACTTAGAAGTGAAGGCG+AGG 0.669805 6_2:-4443147 Msa0905980:three_prime_UTR
TCCAATGACGAGAAAAGTGG+GGG 0.678217 6_2:-4443772 Msa0905980:CDS
TTCCAATGACGAGAAAAGTG+GGG 0.679735 6_2:-4443773 Msa0905980:CDS
CCAACAACGGAAGCTCCACA+AGG 0.691394 6_2:+4443434 None:intergenic
TATAGCTGAAATGAACCTTG+TGG 0.699115 6_2:-4443449 Msa0905980:CDS
CAGATTGAACTAATAGAGGG+CGG 0.706620 6_2:+4443278 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATAAAAAATGTG+TGG + chr6_2:4443315-4443334 None:intergenic 10.0%
!! ATTAACATAACTAATATTTA+AGG - chr6_2:4443437-4443456 Msa0905980:CDS 10.0%
!! GAAGTTTATAAAAAAATAAA+CGG - chr6_2:4443808-4443827 Msa0905980:CDS 10.0%
!!! ATTTTTGTTAATATTTTGAA+TGG + chr6_2:4444950-4444969 None:intergenic 10.0%
!!! GATTAGATTTTTTTATATTT+AGG + chr6_2:4445686-4445705 None:intergenic 10.0%
!!! TGATTTTTATTTATTTTGTA+TGG - chr6_2:4446648-4446667 Msa0905980:intron 10.0%
!!! GATTTTTATTTATTTTGTAT+GGG - chr6_2:4446649-4446668 Msa0905980:intron 10.0%
!!! TCCTAATTTTTAACAAATAT+AGG - chr6_2:4443664-4443683 Msa0905980:CDS 15.0%
!!! TCCTATATTTGTTAAAAATT+AGG + chr6_2:4443668-4443687 None:intergenic 15.0%
!! CAAAATATTAACAAAAATTG+AGG - chr6_2:4444952-4444971 Msa0905980:intron 15.0%
!! GAAAAAAAATGAGAAAAGTA+AGG + chr6_2:4443091-4443110 None:intergenic 20.0%
!!! ATTTTTTTTCTTACTTCCAA+GGG - chr6_2:4443101-4443120 Msa0905980:three_prime_UTR 20.0%
!!! GGTTTTGATTAAATAATTGT+TGG - chr6_2:4443209-4443228 Msa0905980:CDS 20.0%
!! TAAGATTACTCTTTCAAAAT+TGG - chr6_2:4443266-4443285 Msa0905980:CDS 20.0%
!! ATTATTCAAGGCATAAAAAA+GGG + chr6_2:4443501-4443520 None:intergenic 20.0%
!! AATTATTCAAGGCATAAAAA+AGG + chr6_2:4443502-4443521 None:intergenic 20.0%
!! AAACAATCATGAATTATTCA+AGG + chr6_2:4443513-4443532 None:intergenic 20.0%
!!! TTTTTTTCTACAAAAAGTGT+AGG - chr6_2:4443712-4443731 Msa0905980:CDS 20.0%
!!! AGAGATTTTACAATATTGTT+TGG + chr6_2:4443887-4443906 None:intergenic 20.0%
!! ATTAAAAACAATTGAGTCAA+AGG - chr6_2:4443979-4443998 Msa0905980:CDS 20.0%
!!! GGAAAGATTAAATTTTTGTT+AGG + chr6_2:4444732-4444751 None:intergenic 20.0%
!! CAAAAATTTAATCTTTCCAA+AGG - chr6_2:4444734-4444753 Msa0905980:intron 20.0%
!!! TGTTGAAGTTATCATTTTTA+GGG + chr6_2:4445571-4445590 None:intergenic 20.0%
!!! ATGTTGAAGTTATCATTTTT+AGG + chr6_2:4445572-4445591 None:intergenic 20.0%
!!! GTAATATTGGAATTTTAGTT+GGG - chr6_2:4446190-4446209 Msa0905980:intron 20.0%
!! TTTGTAGTGTTAATTAGTTA+GGG - chr6_2:4446394-4446413 Msa0905980:intron 20.0%
!!! TTTCAGTATACTTTGATTTA+GGG - chr6_2:4446747-4446766 Msa0905980:intron 20.0%
!!! CATTTTTTTTCTTACTTCCA+AGG - chr6_2:4443100-4443119 Msa0905980:three_prime_UTR 25.0%
! TTTGTGTTGAAATTCTGATT+TGG - chr6_2:4443188-4443207 Msa0905980:CDS 25.0%
! AAATAAAAAATGTGTGGTTG+TGG + chr6_2:4443309-4443328 None:intergenic 25.0%
!! GTGCATATCAAACTAATTTT+TGG + chr6_2:4443469-4443488 None:intergenic 25.0%
! AGTTATGAAATGTGTTGAAA+TGG + chr6_2:4443766-4443785 None:intergenic 25.0%
! TGTAATGATAGGACATATTT+AGG + chr6_2:4444492-4444511 None:intergenic 25.0%
! TCCTATCATTACATTACATT+CGG - chr6_2:4444499-4444518 Msa0905980:intron 25.0%
!! AGATTAAATTTTTGTTAGGC+TGG + chr6_2:4444728-4444747 None:intergenic 25.0%
! AAAGGAAAAACATGAATGTT+GGG - chr6_2:4444752-4444771 Msa0905980:intron 25.0%
! TTTGAATTTGCCTCATTTAA+AGG + chr6_2:4444880-4444899 None:intergenic 25.0%
! CAAACATTTCATCAAACAAT+TGG - chr6_2:4445526-4445545 Msa0905980:intron 25.0%
! AGCTTTCACTTAAATTATGT+TGG + chr6_2:4445876-4445895 None:intergenic 25.0%
!! ATTTGAAGTTGAAGGAAATT+TGG - chr6_2:4445926-4445945 Msa0905980:intron 25.0%
!!! GGTAATATTGGAATTTTAGT+TGG - chr6_2:4446189-4446208 Msa0905980:intron 25.0%
! CTAACTAATTAACACTACAA+AGG + chr6_2:4446395-4446414 None:intergenic 25.0%
! CTTTGTAGTGTTAATTAGTT+AGG - chr6_2:4446393-4446412 Msa0905980:intron 25.0%
! TTTCAACCAAAATCATTATC+TGG - chr6_2:4446579-4446598 Msa0905980:intron 25.0%
! TTCAACCAAAATCATTATCT+GGG - chr6_2:4446580-4446599 Msa0905980:intron 25.0%
!! TATTTTTCCCTAAATGCAAA+TGG - chr6_2:4446610-4446629 Msa0905980:intron 25.0%
!! ATTTTTCCCTAAATGCAAAT+GGG - chr6_2:4446611-4446630 Msa0905980:intron 25.0%
!! GTTTCAGTATACTTTGATTT+AGG - chr6_2:4446746-4446765 Msa0905980:intron 25.0%
!!! TCATGAAAAATGTGTTGTTT+TGG - chr6_2:4446806-4446825 Msa0905980:intron 25.0%
!!! TTGGAATGAACTTCTTTTTT+CGG - chr6_2:4447283-4447302 Msa0905980:intron 25.0%
! AACATATAAGAGATACATCT+TGG - chr6_2:4447319-4447338 Msa0905980:intron 25.0%
!! TTTTCAGTCATGATAACAAT+TGG - chr6_2:4447838-4447857 Msa0905980:intron 25.0%
ATGAGAAAAGTAAGGATAAG+TGG + chr6_2:4443083-4443102 None:intergenic 30.0%
!! AGGGTTTTATCTGTCTAATT+CGG - chr6_2:4443151-4443170 Msa0905980:three_prime_UTR 30.0%
! TTTTATCTGTCTAATTCGGT+AGG - chr6_2:4443155-4443174 Msa0905980:three_prime_UTR 30.0%
!! CTGATTTTTGTACATTGGTT+AGG + chr6_2:4443413-4443432 None:intergenic 30.0%
!!! TGTCACTGATTTTTGTACAT+TGG + chr6_2:4443418-4443437 None:intergenic 30.0%
! GAAATGTGTTGAAATGGATT+TGG + chr6_2:4443760-4443779 None:intergenic 30.0%
TGTACAACTCAATAAAACCA+AGG - chr6_2:4444072-4444091 Msa0905980:intron 30.0%
CCTATCATTACATTACATTC+GGG - chr6_2:4444500-4444519 Msa0905980:intron 30.0%
AGACAATTGTTGTAGTTTGA+AGG + chr6_2:4444531-4444550 None:intergenic 30.0%
GTTTGAATTTACCATTTGAG+AGG + chr6_2:4444556-4444575 None:intergenic 30.0%
CTCAAATGGTAAATTCAAAC+TGG - chr6_2:4444556-4444575 Msa0905980:intron 30.0%
AAATGAAAAACATGAGTGCT+GGG + chr6_2:4444607-4444626 None:intergenic 30.0%
! TTTAGATGATGGTATTCAAG+TGG - chr6_2:4444655-4444674 Msa0905980:intron 30.0%
! CAACATTCATGTTTTTCCTT+TGG + chr6_2:4444753-4444772 None:intergenic 30.0%
CAAAGGAAAAACATGAATGT+TGG - chr6_2:4444751-4444770 Msa0905980:intron 30.0%
AATTGTCTTGCCTTTAAATG+AGG - chr6_2:4444867-4444886 Msa0905980:intron 30.0%
AATGGAACTATAGAACTTCT+GGG + chr6_2:4444932-4444951 None:intergenic 30.0%
AGATTAAATTCTTGTTAGGC+TGG + chr6_2:4445012-4445031 None:intergenic 30.0%
CGGAAGATTAAATTCTTGTT+AGG + chr6_2:4445016-4445035 None:intergenic 30.0%
! AATGCTTGATGATTGTTGTT+TGG - chr6_2:4445040-4445059 Msa0905980:intron 30.0%
AAATTGTCTTCCTTTGAATG+AGG + chr6_2:4445079-4445098 None:intergenic 30.0%
! AGGAAGACAATTTTTGTAGT+TGG - chr6_2:4445086-4445105 Msa0905980:intron 30.0%
! ATTGTGTAGATTTGAAGCAA+TGG + chr6_2:4445473-4445492 None:intergenic 30.0%
!! GTTGTTTGATTTGAAGTTGA+AGG - chr6_2:4445918-4445937 Msa0905980:intron 30.0%
!!! ATTGGAATTTTAGTTGGGAA+TGG - chr6_2:4446195-4446214 Msa0905980:intron 30.0%
!!! TTGGAATTTTAGTTGGGAAT+GGG - chr6_2:4446196-4446215 Msa0905980:intron 30.0%
TTCAATGAATTTGAGCTCAT+GGG + chr6_2:4446262-4446281 None:intergenic 30.0%
TTTCAATGAATTTGAGCTCA+TGG + chr6_2:4446263-4446282 None:intergenic 30.0%
ACTAATTAACACTACAAAGG+TGG + chr6_2:4446392-4446411 None:intergenic 30.0%
! AACAACCCAGATAATGATTT+TGG + chr6_2:4446588-4446607 None:intergenic 30.0%
AACAAACCCATTTGCATTTA+GGG + chr6_2:4446620-4446639 None:intergenic 30.0%
CAAACAGAAAAATGAAGCAA+TGG + chr6_2:4446687-4446706 None:intergenic 30.0%
GGAATTATTATGAGGATTGT+AGG - chr6_2:4447035-4447054 Msa0905980:intron 30.0%
TTATTATGAGGATTGTAGGT+TGG - chr6_2:4447039-4447058 Msa0905980:intron 30.0%
ATGAAAAAGTTAAAGGATGG+TGG - chr6_2:4447174-4447193 Msa0905980:intron 30.0%
! GAAGGAAAATTGATTCAAGA+AGG - chr6_2:4447344-4447363 Msa0905980:intron 30.0%
! GTCAAGAGAGATTTGATTTA+TGG - chr6_2:4447408-4447427 Msa0905980:intron 30.0%
TCAACACCTTTAATGATGTT+TGG - chr6_2:4447461-4447480 Msa0905980:intron 30.0%
AAATCGCCAAACATCATTAA+AGG + chr6_2:4447470-4447489 None:intergenic 30.0%
!!! TTTTCAAGAAGTTTTGGAAC+CGG - chr6_2:4447487-4447506 Msa0905980:intron 30.0%
! TCATTACCGATTATTTCTTC+CGG + chr6_2:4447509-4447528 None:intergenic 30.0%
TCATTTCAGCTATACAATGT+CGG + chr6_2:4447559-4447578 None:intergenic 30.0%
! AGATAGAGTTTTTGTAGATC+AGG - chr6_2:4447652-4447671 Msa0905980:intron 30.0%
AACCAGATTGAACTAATAGA+GGG + chr6_2:4447742-4447761 None:intergenic 30.0%
! TTTTCTTACTTCCAAGGGTT+TGG - chr6_2:4443106-4443125 Msa0905980:three_prime_UTR 35.0%
! GAGAAGCATGATAGAGTATT+TGG - chr6_2:4443608-4443627 Msa0905980:CDS 35.0%
TTTGGTCGATTATTCTACCT+TGG + chr6_2:4444092-4444111 None:intergenic 35.0%
!!! AGAACACGTTTTTTGGACTT+TGG + chr6_2:4444110-4444129 None:intergenic 35.0%
!!! CAACTCAAGAACACGTTTTT+TGG + chr6_2:4444117-4444136 None:intergenic 35.0%
CCCGAATGTAATGTAATGAT+AGG + chr6_2:4444503-4444522 None:intergenic 35.0%
CAATTGTCTTACCTCTCAAA+TGG - chr6_2:4444542-4444561 Msa0905980:intron 35.0%
ATTCAAACTGGCTAAACATC+AGG - chr6_2:4444568-4444587 Msa0905980:intron 35.0%
CAAATGAAAAACATGAGTGC+TGG + chr6_2:4444608-4444627 None:intergenic 35.0%
! GTTAGACTTGCTTTAGATGA+TGG - chr6_2:4444644-4444663 Msa0905980:intron 35.0%
!! ATGAAGGTATTGAAGTGTCA+AGG + chr6_2:4444700-4444719 None:intergenic 35.0%
TGTACGACAATTGTTGTAGT+TGG - chr6_2:4444823-4444842 Msa0905980:intron 35.0%
GAATGGAACTATAGAACTTC+TGG + chr6_2:4444933-4444952 None:intergenic 35.0%
!! ATGAAGGTATTGAAGTGTCA+AGG + chr6_2:4444700-4444719 None:intergenic 35.0%
!! TGTTAGGCTGGTTTAAATGA+AGG + chr6_2:4445000-4445019 None:intergenic 35.0%
ACAATCATCAAGCATTCACT+CGG + chr6_2:4445036-4445055 None:intergenic 35.0%
! AAATGTGACGAGCATGTTTT+GGG + chr6_2:4445227-4445246 None:intergenic 35.0%
! AAAATGTGACGAGCATGTTT+TGG + chr6_2:4445228-4445247 None:intergenic 35.0%
CAACTGAATGAATTCAACCA+AGG + chr6_2:4445355-4445374 None:intergenic 35.0%
! AGCTTACTTGTTCTTGTTGT+TGG - chr6_2:4445892-4445911 Msa0905980:intron 35.0%
! AGTTGAAGGAAATTTGGAGT+TGG - chr6_2:4445932-4445951 Msa0905980:intron 35.0%
!! GTGTGTGTTGTTTGAAAATG+AGG - chr6_2:4445980-4445999 Msa0905980:intron 35.0%
TACGTGTGTTAACATGCTTA+AGG - chr6_2:4446081-4446100 Msa0905980:intron 35.0%
ACGTGTGTTAACATGCTTAA+GGG - chr6_2:4446082-4446101 Msa0905980:intron 35.0%
AAATCTGCTCTTTACCCAAA+TGG + chr6_2:4446141-4446160 None:intergenic 35.0%
AACTCACACATGGGTAATAT+TGG - chr6_2:4446177-4446196 Msa0905980:intron 35.0%
ACGGTACTTACTTGAAGTTA+GGG + chr6_2:4446363-4446382 None:intergenic 35.0%
AACGGTACTTACTTGAAGTT+AGG + chr6_2:4446364-4446383 None:intergenic 35.0%
ACTACAAAGGTGGTGATAAA+CGG + chr6_2:4446382-4446401 None:intergenic 35.0%
GAACAAACCCATTTGCATTT+AGG + chr6_2:4446621-4446640 None:intergenic 35.0%
ACAGAAAAATGAAGCAATGG+TGG + chr6_2:4446684-4446703 None:intergenic 35.0%
TGATGTTTCTTAATGTGCAG+AGG - chr6_2:4447014-4447033 Msa0905980:intron 35.0%
TGTGCAGAGGAATTATTATG+AGG - chr6_2:4447027-4447046 Msa0905980:intron 35.0%
! AGGATTGTAGGTTGGAATAT+AGG - chr6_2:4447047-4447066 Msa0905980:intron 35.0%
! ATTGTAGGTTGGAATATAGG+AGG - chr6_2:4447050-4447069 Msa0905980:intron 35.0%
! TTGTAGGTTGGAATATAGGA+GGG - chr6_2:4447051-4447070 Msa0905980:intron 35.0%
ATATCCGTGATGTTGTTCAT+AGG - chr6_2:4447099-4447118 Msa0905980:intron 35.0%
TACACCTATGAACAACATCA+CGG + chr6_2:4447106-4447125 None:intergenic 35.0%
TAGGTGTAATCCAATTGTGT+TGG - chr6_2:4447118-4447137 Msa0905980:intron 35.0%
AGGTGTAATCCAATTGTGTT+GGG - chr6_2:4447119-4447138 Msa0905980:intron 35.0%
CTCACGGATGAAAAAGTTAA+AGG - chr6_2:4447167-4447186 Msa0905980:intron 35.0%
CGGATGAAAAAGTTAAAGGA+TGG - chr6_2:4447171-4447190 Msa0905980:intron 35.0%
AAGAGATACATCTTGGTTGA+AGG - chr6_2:4447326-4447345 Msa0905980:intron 35.0%
TATTGACGTGTCTTTCCTAT+TGG - chr6_2:4447367-4447386 Msa0905980:intron 35.0%
!! TGGCGATTTTCAAGAAGTTT+TGG - chr6_2:4447481-4447500 Msa0905980:intron 35.0%
TTGGAACCGGAAGAAATAAT+CGG - chr6_2:4447500-4447519 Msa0905980:intron 35.0%
GGAAGAAATAATCGGTAATG+AGG - chr6_2:4447508-4447527 Msa0905980:intron 35.0%
TATAGCTGAAATGAACCTTG+TGG - chr6_2:4447565-4447584 Msa0905980:intron 35.0%
! GTGAACTTTTTGTCCAACAA+CGG + chr6_2:4447596-4447615 None:intergenic 35.0%
TGTTGGACAAAAAGTTCACA+TGG - chr6_2:4447597-4447616 Msa0905980:intron 35.0%
! GAGTTTTTGTAGATCAGGAA+TGG - chr6_2:4447657-4447676 Msa0905980:intron 35.0%
!!! ATTTCAAGGTTTTGAAGCTG+CGG - chr6_2:4447687-4447706 Msa0905980:intron 35.0%
GAACCAGATTGAACTAATAG+AGG + chr6_2:4447743-4447762 None:intergenic 35.0%
GTTGACATTACAAGCTTATG+TGG - chr6_2:4447767-4447786 Msa0905980:intron 35.0%
CATGATAACAATTGGTATCG+TGG - chr6_2:4447846-4447865 Msa0905980:intron 35.0%
GACACGAATGATGTTGTTGA+GGG + chr6_2:4443865-4443884 None:intergenic 40.0%
ATTCAAGTGGCAACGAAATG+AGG - chr6_2:4444668-4444687 Msa0905980:intron 40.0%
!! TGTTAGGCTGGTTTAGATGA+AGG + chr6_2:4444716-4444735 None:intergenic 40.0%
ATGAATGTTGGGCGAATGTT+GGG - chr6_2:4444763-4444782 Msa0905980:intron 40.0%
!! ATTCAAACTTGCTAAACGCC+TGG + chr6_2:4444791-4444810 None:intergenic 40.0%
GGCAAGACAATTGTTGTAGT+TGG + chr6_2:4444859-4444878 None:intergenic 40.0%
CAGAAGATCGTCATTAGGTA+GGG + chr6_2:4445123-4445142 None:intergenic 40.0%
TGTAACGTTAAGAGGTCTCA+TGG + chr6_2:4445380-4445399 None:intergenic 40.0%
GACCTCTTAACGTTACATCA+AGG - chr6_2:4445383-4445402 Msa0905980:intron 40.0%
TGCCTTGATGTAACGTTAAG+AGG + chr6_2:4445388-4445407 None:intergenic 40.0%
GACGTTCATGCAGAATTCAA+GGG + chr6_2:4445439-4445458 None:intergenic 40.0%
! GTAGATTTGAAGCAATGGTC+AGG + chr6_2:4445468-4445487 None:intergenic 40.0%
! TAACATGCTTAAGGGCATGT+TGG - chr6_2:4446090-4446109 Msa0905980:intron 40.0%
! AACATGCTTAAGGGCATGTT+GGG - chr6_2:4446091-4446110 Msa0905980:intron 40.0%
GCATGTTGGGCAATTACTTT+GGG - chr6_2:4446104-4446123 Msa0905980:intron 40.0%
AGTGTGAGATAACTCACACA+TGG - chr6_2:4446167-4446186 Msa0905980:intron 40.0%
GTGTGAGATAACTCACACAT+GGG - chr6_2:4446168-4446187 Msa0905980:intron 40.0%
! TAGTTGGGAATGGGCAATTT+TGG - chr6_2:4446205-4446224 Msa0905980:intron 40.0%
! GAGAGAGAGTGATTTTGCTA+GGG + chr6_2:4446301-4446320 None:intergenic 40.0%
! AGAGAGAGAGTGATTTTGCT+AGG + chr6_2:4446302-4446321 None:intergenic 40.0%
TACTCAGTCAGGAAAATCTG+TGG - chr6_2:4447076-4447095 Msa0905980:intron 40.0%
TAATCCAATTGTGTTGGGCT+TGG - chr6_2:4447124-4447143 Msa0905980:intron 40.0%
!! AGTTAAAGGATGGTGGAAGA+AGG - chr6_2:4447181-4447200 Msa0905980:intron 40.0%
AGAAGGAGGACAATGTAAAC+TGG - chr6_2:4447198-4447217 Msa0905980:intron 40.0%
GAAGGAGGACAATGTAAACT+GGG - chr6_2:4447199-4447218 Msa0905980:intron 40.0%
TCGTCATTGGAACATGTTTC+TGG + chr6_2:4447233-4447252 None:intergenic 40.0%
TGTTCCAATGACGAGAAAAG+TGG - chr6_2:4447239-4447258 Msa0905980:intron 40.0%
GTTCCAATGACGAGAAAAGT+GGG - chr6_2:4447240-4447259 Msa0905980:intron 40.0%
TTCCAATGACGAGAAAAGTG+GGG - chr6_2:4447241-4447260 Msa0905980:intron 40.0%
AATCTCTCTTGACTGCAACT+CGG + chr6_2:4447401-4447420 None:intergenic 40.0%
!!! TTTCAAGGTTTTGAAGCTGC+GGG - chr6_2:4447688-4447707 Msa0905980:intron 40.0%
CAGATTGAACTAATAGAGGG+CGG + chr6_2:4447739-4447758 None:intergenic 40.0%
CTTATGTGGAAACTCAGACT+TGG - chr6_2:4447781-4447800 Msa0905980:intron 40.0%
ATCGTGGAACTTAGAAGTGA+AGG - chr6_2:4447862-4447881 Msa0905980:intron 40.0%
TCCAAGGGTTTGGCAAGAAA+TGG - chr6_2:4443116-4443135 Msa0905980:three_prime_UTR 45.0%
ACCATTTCTTGCCAAACCCT+TGG + chr6_2:4443120-4443139 None:intergenic 45.0%
AAGGGTTTGGCAAGAAATGG+TGG - chr6_2:4443119-4443138 Msa0905980:three_prime_UTR 45.0%
GGACACGAATGATGTTGTTG+AGG + chr6_2:4443866-4443885 None:intergenic 45.0%
! TGTTCTTGAGTTGTGACCAC+CGG - chr6_2:4444124-4444143 Msa0905980:intron 45.0%
CAAGGTTTAACACTAGCGTG+AGG + chr6_2:4444200-4444219 None:intergenic 45.0%
AGCGTGAGTTAACATACGCA+AGG + chr6_2:4444218-4444237 None:intergenic 45.0%
CATGAATGTTGGGCGAATGT+TGG - chr6_2:4444762-4444781 Msa0905980:intron 45.0%
TGAATGTTGGGCGAATGTTG+GGG - chr6_2:4444764-4444783 Msa0905980:intron 45.0%
GTTTGACCTGCCTCATTCAA+AGG - chr6_2:4445066-4445085 Msa0905980:intron 45.0%
TGTCTTCCTTTGAATGAGGC+AGG + chr6_2:4445075-4445094 None:intergenic 45.0%
GATCGTCATTAGGTAGGGTA+AGG + chr6_2:4445118-4445137 None:intergenic 45.0%
CCAGAAGATCGTCATTAGGT+AGG + chr6_2:4445124-4445143 None:intergenic 45.0%
CCTACCTAATGACGATCTTC+TGG - chr6_2:4445121-4445140 Msa0905980:intron 45.0%
ACCTAATGACGATCTTCTGG+CGG - chr6_2:4445124-4445143 Msa0905980:intron 45.0%
TGTTCCAGTGGATCGATATG+CGG + chr6_2:4445195-4445214 None:intergenic 45.0%
!! GAGAGTATCACTCACTTGAG+TGG + chr6_2:4445266-4445285 None:intergenic 45.0%
AACCTCAGAGGTTGAAGGTT+CGG + chr6_2:4445411-4445430 None:intergenic 45.0%
GTTCATGCAGAATTCAAGGG+CGG + chr6_2:4445436-4445455 None:intergenic 45.0%
CGACGTTCATGCAGAATTCA+AGG + chr6_2:4445440-4445459 None:intergenic 45.0%
AGTCACGCTTCTCTCATAAC+TGG - chr6_2:4446041-4446060 Msa0905980:intron 45.0%
GGCATGTTGGGCAATTACTT+TGG - chr6_2:4446103-4446122 Msa0905980:intron 45.0%
TGGGAATGAGCGAAACCATT+TGG - chr6_2:4446123-4446142 Msa0905980:intron 45.0%
GGGAATGAGCGAAACCATTT+GGG - chr6_2:4446124-4446143 Msa0905980:intron 45.0%
!! TGGGAATGGGCAATTTTGGT+TGG - chr6_2:4446209-4446228 Msa0905980:intron 45.0%
!!! GGGAATGGGCAATTTTGGTT+GGG - chr6_2:4446210-4446229 Msa0905980:intron 45.0%
! TGATTTTGCTAGGGAAGAGG+TGG + chr6_2:4446292-4446311 None:intergenic 45.0%
! GAGTGATTTTGCTAGGGAAG+AGG + chr6_2:4446295-4446314 None:intergenic 45.0%
ATAGGAGGGATTACTCAGTC+AGG - chr6_2:4447065-4447084 Msa0905980:intron 45.0%
TTGACCAAGCCCAACACAAT+TGG + chr6_2:4447131-4447150 None:intergenic 45.0%
!! TAAAGGATGGTGGAAGAAGG+AGG - chr6_2:4447184-4447203 Msa0905980:intron 45.0%
TCCAATGACGAGAAAAGTGG+GGG - chr6_2:4447242-4447261 Msa0905980:intron 45.0%
!! AACTCGGTGCATGAACCAAT+AGG + chr6_2:4447385-4447404 None:intergenic 45.0%
GGTAATGAGGTGACTTCACA+AGG - chr6_2:4447521-4447540 Msa0905980:intron 45.0%
CTCTATCTCTTCTGCTTGCA+CGG + chr6_2:4447640-4447659 None:intergenic 45.0%
! GGAATGGCTAGAGCATTTCA+AGG - chr6_2:4447673-4447692 Msa0905980:intron 45.0%
!!! TTCAAGGTTTTGAAGCTGCG+GGG - chr6_2:4447689-4447708 Msa0905980:intron 45.0%
CGCCCTCTATTAGTTCAATC+TGG - chr6_2:4447737-4447756 Msa0905980:intron 45.0%
AAACTCAGACTTGGACGTGA+AGG - chr6_2:4447790-4447809 Msa0905980:intron 45.0%
!!! TATTATGAATTAAATTTTTT+TGG - chr6_2:4443639-4443658 Msa0905980:CDS 5.0%
!!! ATTAGATTTTTTTATATTTA+GGG + chr6_2:4445685-4445704 None:intergenic 5.0%
! AGAAATGGTGGAGCGTTCTC+AGG - chr6_2:4443131-4443150 Msa0905980:three_prime_UTR 50.0%
! GAAATGGTGGAGCGTTCTCA+GGG - chr6_2:4443132-4443151 Msa0905980:three_prime_UTR 50.0%
TTAACTCACGCCAATGTGGC+CGG + chr6_2:4444146-4444165 None:intergenic 50.0%
CGAGTTAACTCACGCCAATG+TGG + chr6_2:4444150-4444169 None:intergenic 50.0%
AACTCGCGCTAGTGTTAACC+TGG - chr6_2:4444164-4444183 Msa0905980:intron 50.0%
TCCGCCAGAAGATCGTCATT+AGG + chr6_2:4445128-4445147 None:intergenic 50.0%
TTACCCGCATATCGATCCAC+TGG - chr6_2:4445188-4445207 Msa0905980:intron 50.0%
GTTCCAGTGGATCGATATGC+GGG + chr6_2:4445194-4445213 None:intergenic 50.0%
CAAGGAGGGTGGAATACGTT+CGG + chr6_2:4445315-4445334 None:intergenic 50.0%
! GAGCTCATGGGTCTTATGTG+TGG + chr6_2:4446250-4446269 None:intergenic 50.0%
CACGCAACATTCGAGTCTCA+CGG - chr6_2:4447151-4447170 Msa0905980:intron 50.0%
!! TCCCCCACTTTTCTCGTCAT+TGG + chr6_2:4447246-4447265 None:intergenic 50.0%
ATGACGAGAAAAGTGGGGGA+CGG - chr6_2:4447246-4447265 Msa0905980:intron 50.0%
! GACGGGGATTGGTATTCTCT+TGG - chr6_2:4447264-4447283 Msa0905980:intron 50.0%
GGAACTTAGAAGTGAAGGCG+AGG - chr6_2:4447867-4447886 Msa0905980:intron 50.0%
!! CATGAGTGCTGGGCGAATGT+TGG + chr6_2:4444597-4444616 None:intergenic 55.0%
! GGGAGCACAACATGTTCCAG+TGG + chr6_2:4445207-4445226 None:intergenic 55.0%
AAACGTCCAACGCTCGACGA+GGG + chr6_2:4445289-4445308 None:intergenic 55.0%
TTCCACCCTCCTTGTGTCTC+TGG - chr6_2:4445321-4445340 Msa0905980:intron 55.0%
TCCACCCTCCTTGTGTCTCT+GGG - chr6_2:4445322-4445341 Msa0905980:intron 55.0%
CAACCCCAGAGACACAAGGA+GGG + chr6_2:4445329-4445348 None:intergenic 55.0%
TCAACCCCAGAGACACAAGG+AGG + chr6_2:4445330-4445349 None:intergenic 55.0%
GAGTCAACCCCAGAGACACA+AGG + chr6_2:4445333-4445352 None:intergenic 55.0%
!! TGTCTCTGGGGTTGACTCCT+TGG - chr6_2:4445335-4445354 Msa0905980:intron 55.0%
CACCGAACCTTCAACCTCTG+AGG - chr6_2:4445406-4445425 Msa0905980:intron 55.0%
CGGAGAACCTCAGAGGTTGA+AGG + chr6_2:4445416-4445435 None:intergenic 55.0%
TGACGAGAAAAGTGGGGGAC+GGG - chr6_2:4447247-4447266 Msa0905980:intron 55.0%
CCAACAACGGAAGCTCCACA+AGG + chr6_2:4447583-4447602 None:intergenic 55.0%
CCTTGTGGAGCTTCCGTTGT+TGG - chr6_2:4447580-4447599 Msa0905980:intron 55.0%
TGAAGCTGCGGGGATTAGAG+AGG - chr6_2:4447699-4447718 Msa0905980:intron 55.0%
CAGACTTGGACGTGAAGGAG+AGG - chr6_2:4447795-4447814 Msa0905980:intron 55.0%
GTTGTGACCACCGGCCACAT+TGG - chr6_2:4444133-4444152 Msa0905980:intron 60.0%
GCGTGAGGTAACTCACGTCC+AGG + chr6_2:4444185-4444204 None:intergenic 60.0%
TTGGGCGAATGTTGGGGACC+AGG - chr6_2:4444770-4444789 Msa0905980:intron 60.0%
AACGTCCAACGCTCGACGAG+GGG + chr6_2:4445288-4445307 None:intergenic 60.0%
CAAACGTCCAACGCTCGACG+AGG + chr6_2:4445290-4445309 None:intergenic 60.0%
CCACCCTCCTTGTGTCTCTG+GGG - chr6_2:4445323-4445342 Msa0905980:intron 60.0%
TCAAGGGCGGAGAACCTCAG+AGG + chr6_2:4445423-4445442 None:intergenic 60.0%
GACGAGAAAAGTGGGGGACG+GGG - chr6_2:4447248-4447267 Msa0905980:intron 60.0%
GAAAAGTGGGGGACGGGGAT+TGG - chr6_2:4447253-4447272 Msa0905980:intron 60.0%
ACTCACGCCAATGTGGCCGG+TGG + chr6_2:4444143-4444162 None:intergenic 65.0%
ACTCTCCCCTCGTCGAGCGT+TGG - chr6_2:4445280-4445299 Msa0905980:intron 65.0%
CCCCAGAGACACAAGGAGGG+TGG + chr6_2:4445326-4445345 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 4443071 4447965 4443071 ID=Msa0905980;Name=Msa0905980
chr6_2 mRNA 4443071 4447965 4443071 ID=Msa0905980-mRNA-1;Parent=Msa0905980;Name=Msa0905980-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0.5|0.5|0|0.5|2|115|299
chr6_2 exon 4443071 4444002 4443071 ID=Msa0905980-mRNA-1:exon:1291;Parent=Msa0905980-mRNA-1
chr6_2 exon 4447883 4447965 4447883 ID=Msa0905980-mRNA-1:exon:1290;Parent=Msa0905980-mRNA-1
chr6_2 CDS 4447883 4447965 4447883 ID=Msa0905980-mRNA-1:cds;Parent=Msa0905980-mRNA-1
chr6_2 CDS 4443186 4444002 4443186 ID=Msa0905980-mRNA-1:cds;Parent=Msa0905980-mRNA-1
chr6_2 three_prime_UTR 4443071 4443185 4443071 ID=Msa0905980-mRNA-1:three_prime_utr;Parent=Msa0905980-mRNA-1
Gene Sequence

>Msa0905980

ACTCATATTCCACTTATCCTTACTTTTCTCATTTTTTTTCTTACTTCCAAGGGTTTGGCAAGAAATGGTGGAGCGTTCTCAGGAGGAATTATTATGAGGATTGTAGGTTGGAATATAGGAGGGATTACTCAGTCAGGAAAATCTGTGGATATCCGTGATGTTGTTCATAGGTGTAATCCAATTGTGTTGGGCTTGGTCAACACGCAACATTCGAGTCTCACGGATGAAAAAGTTAAAGGATGGTGGAAGAAGGAGGACAATGTAAACTGGGTAGATGTTCCAGAAACATGTTCCAATGACGAGAAAAGTGGGGGACGGGGATTGGTATTCTCTTGGAATGAACTTCTTTTTTCGGAATCAAAAGTTTTAACATATAAGAGATACATCTTGGTTGAAGGAAAATTGATTCAAGAAGGTATTGACGTGTCTTTCCTATTGGTTCATGCACCGAGTTGCAGTCAAGAGAGATTTGATTTATGGAAAGATTTAATGAATCTTAAGAAGTCTATCTCAACACCTTTAATGATGTTTGGCGATTTTCAAGAAGTTTTGGAACCGGAAGAAATAATCGGTAATGAGGTGACTTCACAAGGAATGAAAGACTTCCGACATTGTATAGCTGAAATGAACCTTGTGGAGCTTCCGTTGTTGGACAAAAAGTTCACATGGTATGACTATGACAATAACCGTGCAAGCAGAAGAGATAGAGTTTTTGTAGATCAGGAATGGCTAGAGCATTTCAAGGTTTTGAAGCTGCGGGGATTAGAGAGGTCTCTCTCAGATCACCGCCCTCTATTAGTTCAATCTGGTTCAGTTGTTGACATTACAAGCTTATGTGGAAACTCAGACTTGGACGTGAAGGAGAGGCTTATGCATAAGTTGAAGCTTTTTCAGTCATGA
Protein sequence

>Msa0905980

THIPLILTFLIFFLTSKGLARNGGAFSGGIIMRIVGWNIGGITQSGKSVDIRDVVHRCNPIVLGLVNTQHSSLTDEKVKGWWKKEDNVNWVDVPETCSNDEKSGGRGLVFSWNELLFSESKVLTYKRYILVEGKLIQEGIDVSFLLVHAPSCSQERFDLWKDLMNLKKSISTPLMMFGDFQEVLEPEEIIGNEVTSQGMKDFRHCIAEMNLVELPLLDKKFTWYDYDNNRASRRDRVFVDQEWLEHFKVLKLRGLERSLSDHRPLLVQSGSVVDITSLCGNSDLDVKERLMHKLKLFQS*