Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0916680 | PNY01273.1 | 61.029 | 136 | 28 | 3 | 1 | 111 | 1 | 136 | 1.66e-46 | 156 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0726140 | Msa0916680 | 0.817380 | 3.398733e-52 | -8.615850e-47 |
| Msa0825860 | Msa0916680 | 0.802817 | 4.686037e-49 | -8.615850e-47 |
| Msa0406120 | Msa0916680 | 0.805321 | 1.412622e-49 | -8.615850e-47 |
| Msa0564890 | Msa0916680 | 0.801953 | 7.060446e-49 | -8.615850e-47 |
| Msa0846350 | Msa0916680 | 0.802871 | 4.566929e-49 | -8.615850e-47 |
| Msa0865320 | Msa0916680 | 0.806843 | 6.756131e-50 | -8.615850e-47 |
| Msa0885290 | Msa0916680 | 0.819992 | 8.678881e-53 | -8.615850e-47 |
| Msa0916680 | Msa1285930 | 0.803263 | 3.789232e-49 | -8.615850e-47 |
| Msa0916680 | Msa1287830 | 0.817814 | 2.712923e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0916680 | MtrunA17_Chr8g0386831 | 61.194 | 134 | 20 | 2 | 1 | 111 | 453 | 577 | 3.47e-45 | 153 |
| Msa0916680 | MtrunA17_Chr5g0397411 | 49.286 | 140 | 40 | 3 | 1 | 109 | 451 | 590 | 3.06e-32 | 118 |
| Msa0916680 | MtrunA17_Chr8g0386841 | 52.500 | 120 | 30 | 3 | 1 | 94 | 430 | 548 | 5.48e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0916680 | AT4G11560.1 | 47.000 | 100 | 37 | 1 | 1 | 84 | 467 | 566 | 1.38e-23 | 94.4 |
Find 25 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCGCTTGTGGTTGTTCC+TGG | 0.292028 | 6_2:+38772481 | Msa0916680:CDS |
| TTTGAAGCTCATAAATCATT+TGG | 0.335927 | 6_2:+38772666 | Msa0916680:CDS |
| GATTTATGAGCTTCAAATAT+AGG | 0.347863 | 6_2:-38772660 | None:intergenic |
| GATGAAAATAGCAAAGCTTC+AGG | 0.352961 | 6_2:+38772702 | Msa0916680:CDS |
| TAGCAAAGCTTCAGGGCGTT+TGG | 0.398750 | 6_2:+38772710 | Msa0916680:CDS |
| CTGCATGTGTTGTGGCTCAT+CGG | 0.401201 | 6_2:-38772177 | None:intergenic |
| TTAACGTGTTAACCATATAT+CGG | 0.408741 | 6_2:-38772370 | None:intergenic |
| TGCATGTGTTGTGGCTCATC+GGG | 0.417646 | 6_2:-38772176 | None:intergenic |
| GCAATTTAGAAGTTTGATGT+TGG | 0.425648 | 6_2:+38772582 | Msa0916680:intron |
| AGGACATGCTAACCGATATA+TGG | 0.442148 | 6_2:+38772358 | Msa0916680:CDS |
| CTCATTCTTTGATATTTCCT+CGG | 0.456129 | 6_2:-38772153 | None:intergenic |
| CAATTTAGAAGTTTGATGTT+GGG | 0.469192 | 6_2:+38772583 | Msa0916680:intron |
| CATTGGTCTTGTAAGATTCA+AGG | 0.471102 | 6_2:-38772623 | None:intergenic |
| CGCATCACTAGAGGCAAACC+AGG | 0.504931 | 6_2:-38772499 | None:intergenic |
| AGGCAGCATAAAGATGTCAT+TGG | 0.511497 | 6_2:-38772640 | None:intergenic |
| ATGAAAATAGCAAAGCTTCA+GGG | 0.528128 | 6_2:+38772703 | Msa0916680:CDS |
| ATTGGTCTTGTAAGATTCAA+GGG | 0.528233 | 6_2:-38772622 | None:intergenic |
| CAGCTAGAATGCATCGCTTG+TGG | 0.528679 | 6_2:+38772471 | Msa0916680:intron |
| CGATGAGCCACAACACATGC+AGG | 0.563452 | 6_2:+38772178 | Msa0916680:CDS |
| GTGATGGATATTATCTGTGC+AGG | 0.594177 | 6_2:+38772338 | Msa0916680:CDS |
| CTGGTTTGCCTCTAGTGATG+CGG | 0.616365 | 6_2:+38772500 | Msa0916680:CDS |
| GACAGACACCGCATCACTAG+AGG | 0.621120 | 6_2:-38772508 | None:intergenic |
| CAAGGAGGGTTTGACTGCCG+AGG | 0.633599 | 6_2:+38772136 | None:intergenic |
| TTGGTCTTGTAAGATTCAAG+GGG | 0.641401 | 6_2:-38772621 | None:intergenic |
| AAAGTGACCTGCATGTGTTG+TGG | 0.716814 | 6_2:-38772185 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATACTATTTGTTAAATTG+TGG | + | chr6_2:38772227-38772246 | Msa0916680:intron | 15.0% |
| !!! | TATACTATTTGTTAAATTGT+GGG | + | chr6_2:38772228-38772247 | Msa0916680:intron | 15.0% |
| !! | AACAAATAGTATAAGAATGT+CGG | - | chr6_2:38772221-38772240 | None:intergenic | 20.0% |
| !! | TTGAATATTAGCATTTCTAT+TGG | + | chr6_2:38772262-38772281 | Msa0916680:intron | 20.0% |
| ! | ACAAATAGTATAAGAATGTC+GGG | - | chr6_2:38772220-38772239 | None:intergenic | 25.0% |
| ! | TTAACGTGTTAACCATATAT+CGG | - | chr6_2:38772373-38772392 | None:intergenic | 25.0% |
| !! | CATACATTTCATTTTGAATC+TGG | - | chr6_2:38772549-38772568 | None:intergenic | 25.0% |
| ! | CAATTTAGAAGTTTGATGTT+GGG | + | chr6_2:38772583-38772602 | Msa0916680:intron | 25.0% |
| ! | GATTTATGAGCTTCAAATAT+AGG | - | chr6_2:38772663-38772682 | None:intergenic | 25.0% |
| !! | TTTGAAGCTCATAAATCATT+TGG | + | chr6_2:38772666-38772685 | Msa0916680:CDS | 25.0% |
| CTCATTCTTTGATATTTCCT+CGG | - | chr6_2:38772156-38772175 | None:intergenic | 30.0% | |
| ATTTGTAACCAGCAAAAAGT+TGG | + | chr6_2:38772314-38772333 | Msa0916680:CDS | 30.0% | |
| AAACTTCTAAATTGCCTATG+GGG | - | chr6_2:38772577-38772596 | None:intergenic | 30.0% | |
| CAAACTTCTAAATTGCCTAT+GGG | - | chr6_2:38772578-38772597 | None:intergenic | 30.0% | |
| TCAAACTTCTAAATTGCCTA+TGG | - | chr6_2:38772579-38772598 | None:intergenic | 30.0% | |
| GCAATTTAGAAGTTTGATGT+TGG | + | chr6_2:38772582-38772601 | Msa0916680:intron | 30.0% | |
| !! | ATTGGTCTTGTAAGATTCAA+GGG | - | chr6_2:38772625-38772644 | None:intergenic | 30.0% |
| ATGAAAATAGCAAAGCTTCA+GGG | + | chr6_2:38772703-38772722 | Msa0916680:CDS | 30.0% | |
| ATAGTATAAGAATGTCGGGA+TGG | - | chr6_2:38772216-38772235 | None:intergenic | 35.0% | |
| AATCTGGTGCATCTAATGTT+AGG | - | chr6_2:38772533-38772552 | None:intergenic | 35.0% | |
| !! | TTGGTCTTGTAAGATTCAAG+GGG | - | chr6_2:38772624-38772643 | None:intergenic | 35.0% |
| !! | CATTGGTCTTGTAAGATTCA+AGG | - | chr6_2:38772626-38772645 | None:intergenic | 35.0% |
| GATGAAAATAGCAAAGCTTC+AGG | + | chr6_2:38772702-38772721 | Msa0916680:CDS | 35.0% | |
| !! | GTGATGGATATTATCTGTGC+AGG | + | chr6_2:38772338-38772357 | Msa0916680:CDS | 40.0% |
| AGGACATGCTAACCGATATA+TGG | + | chr6_2:38772358-38772377 | Msa0916680:CDS | 40.0% | |
| GCTGAAGTCATAGAAATAGC+AGG | - | chr6_2:38772430-38772449 | None:intergenic | 40.0% | |
| AAATGTATGCATGACCCCAT+AGG | + | chr6_2:38772560-38772579 | Msa0916680:intron | 40.0% | |
| AGGCAGCATAAAGATGTCAT+TGG | - | chr6_2:38772643-38772662 | None:intergenic | 40.0% | |
| AAAGTGACCTGCATGTGTTG+TGG | - | chr6_2:38772188-38772207 | None:intergenic | 45.0% | |
| ! | CCATCACACCAACTTTTTGC+TGG | - | chr6_2:38772325-38772344 | None:intergenic | 45.0% |
| !! | CCAGCAAAAAGTTGGTGTGA+TGG | + | chr6_2:38772322-38772341 | Msa0916680:CDS | 45.0% |
| TGCATGTGTTGTGGCTCATC+GGG | - | chr6_2:38772179-38772198 | None:intergenic | 50.0% | |
| CTGCATGTGTTGTGGCTCAT+CGG | - | chr6_2:38772180-38772199 | None:intergenic | 50.0% | |
| CAGCTAGAATGCATCGCTTG+TGG | + | chr6_2:38772471-38772490 | Msa0916680:intron | 50.0% | |
| ! | CTGGTTTGCCTCTAGTGATG+CGG | + | chr6_2:38772500-38772519 | Msa0916680:CDS | 50.0% |
| ! | TAGCAAAGCTTCAGGGCGTT+TGG | + | chr6_2:38772710-38772729 | Msa0916680:CDS | 50.0% |
| CGATGAGCCACAACACATGC+AGG | + | chr6_2:38772178-38772197 | Msa0916680:CDS | 55.0% | |
| GCATCGCTTGTGGTTGTTCC+TGG | + | chr6_2:38772481-38772500 | Msa0916680:CDS | 55.0% | |
| CGCATCACTAGAGGCAAACC+AGG | - | chr6_2:38772502-38772521 | None:intergenic | 55.0% | |
| GACAGACACCGCATCACTAG+AGG | - | chr6_2:38772511-38772530 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 38772140 | 38772737 | 38772140 | ID=Msa0916680;Name=Msa0916680 |
| chr6_2 | mRNA | 38772140 | 38772737 | 38772140 | ID=Msa0916680-mRNA-1;Parent=Msa0916680;Name=Msa0916680-mRNA-1;_AED=0.44;_eAED=0.48;_QI=0|0|0|1|0.66|0.5|4|0|113 |
| chr6_2 | exon | 38772140 | 38772199 | 38772140 | ID=Msa0916680-mRNA-1:exon:6415;Parent=Msa0916680-mRNA-1 |
| chr6_2 | exon | 38772293 | 38772379 | 38772293 | ID=Msa0916680-mRNA-1:exon:6416;Parent=Msa0916680-mRNA-1 |
| chr6_2 | exon | 38772474 | 38772521 | 38772474 | ID=Msa0916680-mRNA-1:exon:6417;Parent=Msa0916680-mRNA-1 |
| chr6_2 | exon | 38772591 | 38772737 | 38772591 | ID=Msa0916680-mRNA-1:exon:6418;Parent=Msa0916680-mRNA-1 |
| chr6_2 | CDS | 38772140 | 38772199 | 38772140 | ID=Msa0916680-mRNA-1:cds;Parent=Msa0916680-mRNA-1 |
| chr6_2 | CDS | 38772293 | 38772379 | 38772293 | ID=Msa0916680-mRNA-1:cds;Parent=Msa0916680-mRNA-1 |
| chr6_2 | CDS | 38772474 | 38772521 | 38772474 | ID=Msa0916680-mRNA-1:cds;Parent=Msa0916680-mRNA-1 |
| chr6_2 | CDS | 38772591 | 38772737 | 38772591 | ID=Msa0916680-mRNA-1:cds;Parent=Msa0916680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
