Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1287830 | A0A396J3U9 | 70.248 | 121 | 26 | 3 | 2 | 113 | 1 | 120 | 4.64e-37 | 142 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015750 | Msa1287830 | 0.803335 | 3.660760e-49 | -8.615850e-47 |
| Msa0016570 | Msa1287830 | 0.805633 | 1.214994e-49 | -8.615850e-47 |
| Msa0102070 | Msa1287830 | 0.849992 | 2.264945e-60 | -8.615850e-47 |
| Msa0111510 | Msa1287830 | 0.800961 | 1.127076e-48 | -8.615850e-47 |
| Msa0636170 | Msa1287830 | 0.817410 | 3.345675e-52 | -8.615850e-47 |
| Msa0678930 | Msa1287830 | 0.818134 | 2.297086e-52 | -8.615850e-47 |
| Msa0726140 | Msa1287830 | 0.864713 | 9.973518e-65 | -8.615850e-47 |
| Msa0729800 | Msa1287830 | 0.814577 | 1.435204e-51 | -8.615850e-47 |
| Msa0733290 | Msa1287830 | 0.847687 | 9.874487e-60 | -8.615850e-47 |
| Msa0743810 | Msa1287830 | 0.889440 | 2.417770e-73 | -8.615850e-47 |
| Msa0745060 | Msa1287830 | 0.871138 | 8.585978e-67 | -8.615850e-47 |
| Msa0767720 | Msa1287830 | 0.804994 | 1.653261e-49 | -8.615850e-47 |
| Msa0783160 | Msa1287830 | 0.822255 | 2.611644e-53 | -8.615850e-47 |
| Msa0787600 | Msa1287830 | 0.832019 | 1.200272e-55 | -8.615850e-47 |
| Msa0808010 | Msa1287830 | 0.832473 | 9.263746e-56 | -8.615850e-47 |
| Msa0808020 | Msa1287830 | 0.828781 | 7.427038e-55 | -8.615850e-47 |
| Msa0825860 | Msa1287830 | 0.879694 | 1.010959e-69 | -8.615850e-47 |
| Msa0833290 | Msa1287830 | 0.803371 | 3.598691e-49 | -8.615850e-47 |
| Msa0145750 | Msa1287830 | 0.812037 | 5.185919e-51 | -8.615850e-47 |
| Msa0153910 | Msa1287830 | 0.815333 | 9.752842e-52 | -8.615850e-47 |
| Msa0200000 | Msa1287830 | 0.826834 | 2.182269e-54 | -8.615850e-47 |
| Msa0227480 | Msa1287830 | 0.817467 | 3.248316e-52 | -8.615850e-47 |
| Msa0232850 | Msa1287830 | 0.810455 | 1.142975e-50 | -8.615850e-47 |
| Msa0235140 | Msa1287830 | 0.837013 | 6.676781e-57 | -8.615850e-47 |
| Msa1260100 | Msa1287830 | 0.813644 | 2.305702e-51 | -8.615850e-47 |
| Msa1269670 | Msa1287830 | 0.866047 | 3.794127e-65 | -8.615850e-47 |
| Msa1270230 | Msa1287830 | 0.855906 | 4.605650e-62 | -8.615850e-47 |
| Msa1270240 | Msa1287830 | 0.836732 | 7.873718e-57 | -8.615850e-47 |
| Msa1270270 | Msa1287830 | 0.868451 | 6.466721e-66 | -8.615850e-47 |
| Msa1283330 | Msa1287830 | 0.849707 | 2.721184e-60 | -8.615850e-47 |
| Msa1285930 | Msa1287830 | 0.909070 | 8.543269e-82 | -8.615850e-47 |
| Msa1287800 | Msa1287830 | 0.895474 | 9.264406e-76 | -8.615850e-47 |
| Msa1287810 | Msa1287830 | 0.903589 | 2.968220e-79 | -8.615850e-47 |
| Msa1287820 | Msa1287830 | 0.829800 | 4.203663e-55 | -8.615850e-47 |
| Msa1287830 | Msa1289430 | 0.830991 | 2.149559e-55 | -8.615850e-47 |
| Msa1287830 | Msa1297210 | 0.831818 | 1.345360e-55 | -8.615850e-47 |
| Msa1287830 | Msa1305480 | 0.804676 | 1.927031e-49 | -8.615850e-47 |
| Msa1287830 | Msa1307890 | 0.824830 | 6.525697e-54 | -8.615850e-47 |
| Msa1287830 | Msa1310120 | 0.808608 | 2.848441e-50 | -8.615850e-47 |
| Msa1287830 | Msa1320300 | 0.839115 | 1.921279e-57 | -8.615850e-47 |
| Msa1287830 | Msa1340590 | 0.839568 | 1.465802e-57 | -8.615850e-47 |
| Msa1287830 | Msa1342200 | 0.813002 | 3.190276e-51 | -8.615850e-47 |
| Msa1287830 | Msa1369430 | 0.802367 | 5.801768e-49 | -8.615850e-47 |
| Msa1287830 | Msa1397580 | 0.830745 | 2.470633e-55 | -8.615850e-47 |
| Msa1287830 | Msa1404000 | 0.851299 | 9.715352e-61 | -8.615850e-47 |
| Msa1287830 | Msa1408780 | 0.825294 | 5.070135e-54 | -8.615850e-47 |
| Msa1287830 | Msa1415310 | 0.851948 | 6.362980e-61 | -8.615850e-47 |
| Msa1287830 | Msa1422180 | 0.819932 | 8.956784e-53 | -8.615850e-47 |
| Msa1287830 | Msa1433120 | 0.857244 | 1.863245e-62 | -8.615850e-47 |
| Msa0365500 | Msa1287830 | 0.825416 | 4.743277e-54 | -8.615850e-47 |
| Msa0366580 | Msa1287830 | 0.843279 | 1.544944e-58 | -8.615850e-47 |
| Msa0384800 | Msa1287830 | 0.828796 | 7.364604e-55 | -8.615850e-47 |
| Msa0394970 | Msa1287830 | 0.805015 | 1.637076e-49 | -8.615850e-47 |
| Msa0396600 | Msa1287830 | 0.800821 | 1.204053e-48 | -8.615850e-47 |
| Msa0406120 | Msa1287830 | 0.861461 | 1.009326e-63 | -8.615850e-47 |
| Msa0414410 | Msa1287830 | 0.842505 | 2.482198e-58 | -8.615850e-47 |
| Msa0414420 | Msa1287830 | 0.869319 | 3.385063e-66 | -8.615850e-47 |
| Msa0438620 | Msa1287830 | 0.824851 | 6.451095e-54 | -8.615850e-47 |
| Msa0450000 | Msa1287830 | 0.817208 | 3.714249e-52 | -8.615850e-47 |
| Msa0452900 | Msa1287830 | 0.871372 | 7.186707e-67 | -8.615850e-47 |
| Msa0452910 | Msa1287830 | 0.832025 | 1.196086e-55 | -8.615850e-47 |
| Msa0453120 | Msa1287830 | 0.814330 | 1.627894e-51 | -8.615850e-47 |
| Msa0454370 | Msa1287830 | 0.803701 | 3.074788e-49 | -8.615850e-47 |
| Msa0454910 | Msa1287830 | 0.823786 | 1.148447e-53 | -8.615850e-47 |
| Msa0459160 | Msa1287830 | 0.815709 | 8.045447e-52 | -8.615850e-47 |
| Msa0459170 | Msa1287830 | 0.811612 | 6.418108e-51 | -8.615850e-47 |
| Msa0981370 | Msa1287830 | 0.836042 | 1.179728e-56 | -8.615850e-47 |
| Msa0989060 | Msa1287830 | 0.842135 | 3.110186e-58 | -8.615850e-47 |
| Msa1016280 | Msa1287830 | 0.855538 | 5.900703e-62 | -8.615850e-47 |
| Msa1024240 | Msa1287830 | 0.811468 | 6.897016e-51 | -8.615850e-47 |
| Msa1025360 | Msa1287830 | 0.866932 | 1.986253e-65 | -8.615850e-47 |
| Msa1025370 | Msa1287830 | 0.868311 | 7.173661e-66 | -8.615850e-47 |
| Msa1044830 | Msa1287830 | 0.877411 | 6.422141e-69 | -8.615850e-47 |
| Msa1044840 | Msa1287830 | 0.870876 | 1.047608e-66 | -8.615850e-47 |
| Msa1077460 | Msa1287830 | 0.800636 | 1.313030e-48 | -8.615850e-47 |
| Msa1092990 | Msa1287830 | 0.833945 | 3.983210e-56 | -8.615850e-47 |
| Msa1096580 | Msa1287830 | 0.809556 | 1.784699e-50 | -8.615850e-47 |
| Msa1100980 | Msa1287830 | 0.864258 | 1.383828e-64 | -8.615850e-47 |
| Msa0267000 | Msa1287830 | 0.870497 | 1.396035e-66 | -8.615850e-47 |
| Msa0267010 | Msa1287830 | 0.864922 | 8.580397e-65 | -8.615850e-47 |
| Msa0313760 | Msa1287830 | 0.837174 | 6.072485e-57 | -8.615850e-47 |
| Msa0342240 | Msa1287830 | 0.852386 | 4.775111e-61 | -8.615850e-47 |
| Msa0360510 | Msa1287830 | 0.828044 | 1.118987e-54 | -8.615850e-47 |
| Msa0363300 | Msa1287830 | 0.811048 | 8.506870e-51 | -8.615850e-47 |
| Msa1109440 | Msa1287830 | 0.855819 | 4.885483e-62 | -8.615850e-47 |
| Msa1121200 | Msa1287830 | 0.828354 | 9.422166e-55 | -8.615850e-47 |
| Msa1135740 | Msa1287830 | 0.846573 | 1.995316e-59 | -8.615850e-47 |
| Msa1136090 | Msa1287830 | 0.806666 | 7.363024e-50 | -8.615850e-47 |
| Msa1146110 | Msa1287830 | 0.855045 | 8.210914e-62 | -8.615850e-47 |
| Msa1150370 | Msa1287830 | 0.842592 | 2.353212e-58 | -8.615850e-47 |
| Msa1164120 | Msa1287830 | 0.810745 | 9.892449e-51 | -8.615850e-47 |
| Msa1173920 | Msa1287830 | 0.857329 | 1.758203e-62 | -8.615850e-47 |
| Msa1187940 | Msa1287830 | 0.854846 | 9.375055e-62 | -8.615850e-47 |
| Msa1187950 | Msa1287830 | 0.824029 | 1.007031e-53 | -8.615850e-47 |
| Msa1192640 | Msa1287830 | 0.896834 | 2.521880e-76 | -8.615850e-47 |
| Msa0491590 | Msa1287830 | 0.840570 | 8.029280e-58 | -8.615850e-47 |
| Msa0502760 | Msa1287830 | 0.802413 | 5.676063e-49 | -8.615850e-47 |
| Msa0548060 | Msa1287830 | 0.807135 | 5.859670e-50 | -8.615850e-47 |
| Msa0564890 | Msa1287830 | 0.871242 | 7.931554e-67 | -8.615850e-47 |
| Msa0846210 | Msa1287830 | 0.804299 | 2.309233e-49 | -8.615850e-47 |
| Msa0846330 | Msa1287830 | 0.833768 | 4.410549e-56 | -8.615850e-47 |
| Msa0846350 | Msa1287830 | 0.828256 | 9.944769e-55 | -8.615850e-47 |
| Msa0865320 | Msa1287830 | 0.876363 | 1.482649e-68 | -8.615850e-47 |
| Msa0885290 | Msa1287830 | 0.852669 | 3.966166e-61 | -8.615850e-47 |
| Msa0887920 | Msa1287830 | 0.803636 | 3.171661e-49 | -8.615850e-47 |
| Msa0888370 | Msa1287830 | 0.831108 | 2.011650e-55 | -8.615850e-47 |
| Msa0916680 | Msa1287830 | 0.817814 | 2.712923e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1287830 | MtrunA17_Chr2g0289121 | 70.248 | 121 | 26 | 3 | 2 | 113 | 1 | 120 | 8.93e-41 | 142 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 4 sgRNAs with CRISPR-Local
Find 6 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAATGATAAATATTGTATT+TGG | 0.252954 | 8_3:-39419758 | Msa1287830:CDS |
| ACTACGATAGTTCTTTGATT+TGG | 0.343751 | 8_3:+39419464 | None:intergenic |
| GATAAATATTGTATTTGGTG+CGG | 0.569850 | 8_3:-39419753 | Msa1287830:CDS |
| AAGATTAAAATTTCAAGACA+AGG | 0.606952 | 8_3:-39419722 | Msa1287830:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGATTAAAATTTCAAGACA+AGG | - | chr8_3:39419446-39419465 | Msa1287830:CDS | 20.0% |
| !! | GATAAATATTGTATTTGGTG+CGG | - | chr8_3:39419415-39419434 | Msa1287830:CDS | 25.0% |
| !! | AATCAACAATCTGAGTTTTT+AGG | - | chr8_3:39419476-39419495 | Msa1287830:CDS | 25.0% |
| !! | ATCAACAATCTGAGTTTTTA+GGG | - | chr8_3:39419477-39419496 | Msa1287830:CDS | 25.0% |
| ACTACGATAGTTCTTTGATT+TGG | + | chr8_3:39419707-39419726 | None:intergenic | 30.0% | |
| ! | TTGTGACGATTTTGCTGCAG+AGG | + | chr8_3:39419590-39419609 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 39419413 | 39419777 | 39419413 | ID=Msa1287830;Name=Msa1287830 |
| chr8_3 | mRNA | 39419413 | 39419777 | 39419413 | ID=Msa1287830-mRNA-1;Parent=Msa1287830;Name=Msa1287830-mRNA-1;_AED=0.49;_eAED=0.52;_QI=0|0|0|1|0|0|2|0|113 |
| chr8_3 | exon | 39419692 | 39419777 | 39419692 | ID=Msa1287830-mRNA-1:exon:11047;Parent=Msa1287830-mRNA-1 |
| chr8_3 | exon | 39419413 | 39419668 | 39419413 | ID=Msa1287830-mRNA-1:exon:11046;Parent=Msa1287830-mRNA-1 |
| chr8_3 | CDS | 39419692 | 39419777 | 39419692 | ID=Msa1287830-mRNA-1:cds;Parent=Msa1287830-mRNA-1 |
| chr8_3 | CDS | 39419413 | 39419668 | 39419413 | ID=Msa1287830-mRNA-1:cds;Parent=Msa1287830-mRNA-1 |
| Gene Sequence |
| Protein sequence |
