AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0966080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 11 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATCTAATGTCCTTAT+TGG 0.159858 6_3:-94025525 None:intergenic
AATGTCCTTATTGGTTGTTT+TGG 0.290172 6_3:-94025516 Msa0966080:CDS
TTCATCCAAAACAACCAATA+AGG 0.361690 6_3:+94025511 None:intergenic
CTCTGAATCTGAACAGTCTT+AGG 0.361731 6_3:+94025440 None:intergenic
CTTAGGCCACTTGTCGAATC+TGG 0.370934 6_3:+94025457 None:intergenic
TTCAGATTCAGAGGAAGAAG+AGG 0.501652 6_3:-94025430 Msa0966080:CDS
TTAACATGGTTTAATCATTG+AGG 0.522179 6_3:+94025110 None:intergenic
GGAAGCTATTCGAGAAGAAG+AGG 0.554823 6_3:-94025487 Msa0966080:CDS
CTAAGACTGTTCAGATTCAG+AGG 0.581573 6_3:-94025439 Msa0966080:CDS
ATTTGACCAGATTCGACAAG+TGG 0.608851 6_3:-94025463 Msa0966080:CDS
GAAGCTATTCGAGAAGAAGA+GGG 0.626506 6_3:-94025486 Msa0966080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTCATCCAAAACAACCAATA+AGG + chr6_3:94025131-94025150 None:intergenic 30.0%
!!! TATTGGTTGTTTTGGATGAA+TGG - chr6_3:94025131-94025150 Msa0966080:CDS 30.0%
AAACCATATAGACTGATACA+CGG + chr6_3:94025349-94025368 None:intergenic 30.0%
! TATCAGTCTATATGGTTTGT+AGG - chr6_3:94025351-94025370 Msa0966080:intron 30.0%
TTTCAACATTTAGCATGTAC+TGG - chr6_3:94025455-94025474 Msa0966080:CDS 30.0%
ATATAGACTGATACACGGAT+AGG + chr6_3:94025344-94025363 None:intergenic 35.0%
TATCCGTGTATCAGTCTATA+TGG - chr6_3:94025343-94025362 Msa0966080:intron 35.0%
GAAGCTATTCGAGAAGAAGA+GGG - chr6_3:94025153-94025172 Msa0966080:intron 40.0%
ATTTGACCAGATTCGACAAG+TGG - chr6_3:94025176-94025195 Msa0966080:intron 40.0%
CTCTGAATCTGAACAGTCTT+AGG + chr6_3:94025202-94025221 None:intergenic 40.0%
CTAAGACTGTTCAGATTCAG+AGG - chr6_3:94025200-94025219 Msa0966080:intron 40.0%
TTCAGATTCAGAGGAAGAAG+AGG - chr6_3:94025209-94025228 Msa0966080:intron 40.0%
!!! AGAAGAGGATTTTGAAGCAG+AGG - chr6_3:94025224-94025243 Msa0966080:intron 40.0%
!!! GATTTTGAAGCAGAGGTTGA+AGG - chr6_3:94025231-94025250 Msa0966080:intron 40.0%
! CCAAATTTCTAGACAGCTCA+AGG + chr6_3:94025282-94025301 None:intergenic 40.0%
CCTTGAGCTGTCTAGAAATT+TGG - chr6_3:94025279-94025298 Msa0966080:intron 40.0%
GCTGTCTAGAAATTTGGATG+TGG - chr6_3:94025285-94025304 Msa0966080:intron 40.0%
GGAAGCTATTCGAGAAGAAG+AGG - chr6_3:94025152-94025171 Msa0966080:CDS 45.0%
CTTAGGCCACTTGTCGAATC+TGG + chr6_3:94025185-94025204 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 94025124 94025537 94025124 ID=Msa0966080;Name=Msa0966080
chr6_3 mRNA 94025124 94025537 94025124 ID=Msa0966080-mRNA-1;Parent=Msa0966080;Name=Msa0966080-mRNA-1;_AED=0.72;_eAED=0.72;_QI=0|0|0|0.5|1|1|2|0|58
chr6_3 exon 94025409 94025537 94025409 ID=Msa0966080-mRNA-1:exon:17338;Parent=Msa0966080-mRNA-1
chr6_3 exon 94025124 94025171 94025124 ID=Msa0966080-mRNA-1:exon:17337;Parent=Msa0966080-mRNA-1
chr6_3 CDS 94025409 94025537 94025409 ID=Msa0966080-mRNA-1:cds;Parent=Msa0966080-mRNA-1
chr6_3 CDS 94025124 94025171 94025124 ID=Msa0966080-mRNA-1:cds;Parent=Msa0966080-mRNA-1
Gene Sequence

>Msa0966080

ATGTCCTTATTGGTTGTTTTGGATGAATGGAAGCTATTCGAGAAGAAGAGGGATTTGACCAGATTCGACAAGTGGCCTAAGACTGTTCAGATTCAGAGGAAGAAGAGGATTTTGAAGCAGAGGTTGAAGCAGCTGTCAGTTGCATTATGTTACAACTTGCTGTCATCACCTCAATGA

Protein sequence

>Msa0966080

MSLLVVLDEWKLFEKKRDLTRFDKWPKTVQIQRKKRILKQRLKQLSVALCYNLLSSPQ*