Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0610800 | Msa0997110 | 0.807886 | 4.059216e-50 | -8.615850e-47 |
| Msa0808010 | Msa0997110 | 0.801467 | 8.883306e-49 | -8.615850e-47 |
| Msa0997110 | Msa1121480 | 0.814466 | 1.518700e-51 | -8.615850e-47 |
| Msa0997110 | Msa1121490 | 0.802238 | 6.168557e-49 | -8.615850e-47 |
| Msa0545700 | Msa0997110 | 0.800726 | 1.258684e-48 | -8.615850e-47 |
| Msa0594560 | Msa0997110 | 0.804527 | 2.070077e-49 | -8.615850e-47 |
| Msa0844490 | Msa0997110 | 0.804687 | 1.916973e-49 | -8.615850e-47 |
| Msa0925290 | Msa0997110 | 0.802203 | 6.269979e-49 | -8.615850e-47 |
| Msa0927380 | Msa0997110 | 0.810628 | 1.048715e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGAACAGATTGAGATTT+TGG | 0.155235 | 6_4:+76196298 | Msa0997110:CDS |
| CCTCAAAGGAATGACTAATT+AGG | 0.261604 | 6_4:-76196108 | None:intergenic |
| TGTTAAGAATGCTTTGGATT+TGG | 0.295341 | 6_4:+76196651 | Msa0997110:CDS |
| GAAACCAACGTAGATGAAAT+TGG | 0.297664 | 6_4:+76196130 | Msa0997110:CDS |
| CCTAATTAGTCATTCCTTTG+AGG | 0.320162 | 6_4:+76196108 | Msa0997110:CDS |
| CAACGTAGATGAAATTGGTT+TGG | 0.382676 | 6_4:+76196135 | Msa0997110:CDS |
| TGATAACAATCTTTCGTCAT+TGG | 0.390779 | 6_4:+76196165 | Msa0997110:CDS |
| TTGCAGTGTTAAGAATGCTT+TGG | 0.416508 | 6_4:+76196645 | Msa0997110:intron |
| ACCACCTTTACAAGCTAGTC+TGG | 0.480028 | 6_4:-76196332 | None:intergenic |
| ATCTACGTTGGTTTCCTCAA+AGG | 0.502573 | 6_4:-76196122 | None:intergenic |
| CAAACCAATTTCATCTACGT+TGG | 0.546211 | 6_4:-76196134 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAATTTATGTTTGCTATAA+TGG | + | chr6_4:76196463-76196482 | Msa0997110:intron | 20.0% |
| !!! | CTTTTGTTCTTTTAGAGTAT+CGG | + | chr6_4:76196404-76196423 | Msa0997110:intron | 25.0% |
| !!! | TACTTTGAAATAAATGACAG+CGG | - | chr6_4:76196564-76196583 | None:intergenic | 25.0% |
| TGATAACAATCTTTCGTCAT+TGG | + | chr6_4:76196165-76196184 | Msa0997110:CDS | 30.0% | |
| GGTGTGATTAACTCTATATT+TGG | + | chr6_4:76196186-76196205 | Msa0997110:intron | 30.0% | |
| ! | TGAAGAACAGATTGAGATTT+TGG | + | chr6_4:76196298-76196317 | Msa0997110:CDS | 30.0% |
| !! | AAATAAATGACAGCGGATTT+TGG | - | chr6_4:76196557-76196576 | None:intergenic | 30.0% |
| ! | TGTTAAGAATGCTTTGGATT+TGG | + | chr6_4:76196651-76196670 | Msa0997110:CDS | 30.0% |
| CCTCAAAGGAATGACTAATT+AGG | - | chr6_4:76196111-76196130 | None:intergenic | 35.0% | |
| CCTAATTAGTCATTCCTTTG+AGG | + | chr6_4:76196108-76196127 | Msa0997110:CDS | 35.0% | |
| GAAACCAACGTAGATGAAAT+TGG | + | chr6_4:76196130-76196149 | Msa0997110:CDS | 35.0% | |
| CAAACCAATTTCATCTACGT+TGG | - | chr6_4:76196137-76196156 | None:intergenic | 35.0% | |
| CAACGTAGATGAAATTGGTT+TGG | + | chr6_4:76196135-76196154 | Msa0997110:CDS | 35.0% | |
| CTAATCTTATTCCTCCCAAT+AGG | + | chr6_4:76196269-76196288 | Msa0997110:intron | 35.0% | |
| !!! | TTAGAGTATCGGTCTCTTTT+AGG | + | chr6_4:76196415-76196434 | Msa0997110:intron | 35.0% |
| !!! | TAGAGTATCGGTCTCTTTTA+GGG | + | chr6_4:76196416-76196435 | Msa0997110:intron | 35.0% |
| TTGCAGTGTTAAGAATGCTT+TGG | + | chr6_4:76196645-76196664 | Msa0997110:intron | 35.0% | |
| !! | ATCTACGTTGGTTTCCTCAA+AGG | - | chr6_4:76196125-76196144 | None:intergenic | 40.0% |
| GTTCTTCAGCTTCTCCTATT+GGG | - | chr6_4:76196286-76196305 | None:intergenic | 40.0% | |
| TGTTCTTCAGCTTCTCCTAT+TGG | - | chr6_4:76196287-76196306 | None:intergenic | 40.0% | |
| CTTGTAAAGGTGGTTTCTTC+TGG | + | chr6_4:76196341-76196360 | Msa0997110:intron | 40.0% | |
| !! | TGTGCCAGACTAGCTTGTAA+AGG | + | chr6_4:76196328-76196347 | Msa0997110:intron | 45.0% |
| ACCACCTTTACAAGCTAGTC+TGG | - | chr6_4:76196335-76196354 | None:intergenic | 45.0% | |
| ! | CTTCTGGAGACACTGTGTAA+CGG | + | chr6_4:76196357-76196376 | Msa0997110:intron | 45.0% |
| CTTCAGCTTCTCCTATTGGG+AGG | - | chr6_4:76196283-76196302 | None:intergenic | 50.0% | |
| !! | GCCAGACTAGCTTGTAAAGG+TGG | + | chr6_4:76196331-76196350 | Msa0997110:intron | 50.0% |
| TGGAGACACTGTGTAACGGT+GGG | + | chr6_4:76196361-76196380 | Msa0997110:intron | 50.0% | |
| CTGGAGACACTGTGTAACGG+TGG | + | chr6_4:76196360-76196379 | Msa0997110:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 76196097 | 76196687 | 76196097 | ID=Msa0997110;Name=Msa0997110 |
| chr6_4 | mRNA | 76196097 | 76196687 | 76196097 | ID=Msa0997110-mRNA-1;Parent=Msa0997110;Name=Msa0997110-mRNA-1;_AED=0.20;_eAED=0.45;_QI=0|0|0|1|0|0|3|0|59 |
| chr6_4 | exon | 76196097 | 76196186 | 76196097 | ID=Msa0997110-mRNA-1:exon:14363;Parent=Msa0997110-mRNA-1 |
| chr6_4 | exon | 76196291 | 76196343 | 76196291 | ID=Msa0997110-mRNA-1:exon:14364;Parent=Msa0997110-mRNA-1 |
| chr6_4 | exon | 76196651 | 76196687 | 76196651 | ID=Msa0997110-mRNA-1:exon:14365;Parent=Msa0997110-mRNA-1 |
| chr6_4 | CDS | 76196097 | 76196186 | 76196097 | ID=Msa0997110-mRNA-1:cds;Parent=Msa0997110-mRNA-1 |
| chr6_4 | CDS | 76196291 | 76196343 | 76196291 | ID=Msa0997110-mRNA-1:cds;Parent=Msa0997110-mRNA-1 |
| chr6_4 | CDS | 76196651 | 76196687 | 76196651 | ID=Msa0997110-mRNA-1:cds;Parent=Msa0997110-mRNA-1 |
| Gene Sequence |
| Protein sequence |
