AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1000240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1000240 MtrunA17_Chr6g0486531 63.291 79 24 1 1 74 424 502 1.27e-21 88.6
Msa1000240 MtrunA17_Chr6g0486531 69.048 42 13 0 75 116 619 660 4.52e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCTGTCTGTCTCAGTTTC+AGG 0.204785 6_4:+82949596 None:intergenic
ACATGAGCTCCTAAGGAAAA+AGG 0.318240 6_4:-82948451 Msa1000240:CDS
AAGCAACTTCAGGACGAAAC+AGG 0.454192 6_4:-82948534 Msa1000240:intron
CTTCAGGACGAAACAGGATC+TGG 0.468831 6_4:-82948528 Msa1000240:intron
TCAAAGCACATGAGCTCCTA+AGG 0.487568 6_4:-82948458 Msa1000240:CDS
GATGAAAGTGTACTAGAAAG+TGG 0.503380 6_4:-82949686 Msa1000240:CDS
CGAAACAGGATCTGGAATGA+TGG 0.515352 6_4:-82948520 Msa1000240:CDS
GATTCTGTAACAACAGGGCT+AGG 0.516573 6_4:+82949563 None:intergenic
CTCATGTGCTTTGATAATGT+CGG 0.550385 6_4:+82948467 None:intergenic
AACTGACTCACTTGTTGAAG+TGG 0.554070 6_4:-82949711 Msa1000240:CDS
GGCTGTAAATTCAATTACTG+AGG 0.629591 6_4:-82949771 Msa1000240:CDS
TCAGGAAATCAACACAGTAG+TGG 0.648084 6_4:-82947279 None:intergenic
AGTTTGTGCGGTGACTTCAG+TGG 0.696642 6_4:+82948417 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATATTTTTCTATAAAGA+TGG - chr6_4:82949039-82949058 Msa1000240:intron 10.0%
!! AAAATTTCAATTAAAGTTAA+TGG - chr6_4:82949854-82949873 Msa1000240:intron 10.0%
!! AAAAAAAAAAAAACACTTCA+TGG + chr6_4:82947308-82947327 None:intergenic 15.0%
!! TAAGCTTAATAAAAATTCAT+GGG + chr6_4:82947423-82947442 None:intergenic 15.0%
!!! ATTGATTTTAGTTGTAATTT+CGG + chr6_4:82948300-82948319 None:intergenic 15.0%
!! GAAACTTTAAATTAAGTTAA+AGG + chr6_4:82949444-82949463 None:intergenic 15.0%
!!! AAATATGGATATTTTAGAAT+GGG + chr6_4:82949898-82949917 None:intergenic 15.0%
!!! TAAATATGGATATTTTAGAA+TGG + chr6_4:82949899-82949918 None:intergenic 15.0%
!! TATTATCTATGAATTATTCT+TGG - chr6_4:82950181-82950200 Msa1000240:intron 15.0%
!! CTAAGCTTAATAAAAATTCA+TGG + chr6_4:82947424-82947443 None:intergenic 20.0%
!! TCACATTCAATTCATATAAT+AGG + chr6_4:82947671-82947690 None:intergenic 20.0%
!!! TTTGAAACTAAAAATATCCA+AGG + chr6_4:82947766-82947785 None:intergenic 20.0%
!! AAAACAAAAAATAACCTTCA+TGG + chr6_4:82947796-82947815 None:intergenic 20.0%
!! AAAATCAAGACAAAACAAAA+GGG + chr6_4:82947856-82947875 None:intergenic 20.0%
!! AAAAATCAAGACAAAACAAA+AGG + chr6_4:82947857-82947876 None:intergenic 20.0%
!!! ATTTTCTTTAACTTTGAATC+AGG - chr6_4:82948606-82948625 Msa1000240:intron 20.0%
!!! CTTAATTTAAAGTTTCAAAC+AGG - chr6_4:82949448-82949467 Msa1000240:intron 20.0%
!!! GATAGTTTACTATCTTTTTT+TGG + chr6_4:82949828-82949847 None:intergenic 20.0%
!!! CATTAACCTTATTTACTAAT+TGG - chr6_4:82949947-82949966 Msa1000240:intron 20.0%
!! TTAAGTGAAAGAAAAATAGT+AGG + chr6_4:82950009-82950028 None:intergenic 20.0%
!! CTTTTTGTGTTTCAAGAAAT+CGG - chr6_4:82947565-82947584 Msa1000240:intron 25.0%
! TTCAATTCATATAATAGGCA+AGG + chr6_4:82947666-82947685 None:intergenic 25.0%
! TCAAAATTCAAACTCAGTTA+TGG + chr6_4:82947701-82947720 None:intergenic 25.0%
!!! GGTTATTTTTTGTTTTTCTG+TGG - chr6_4:82947800-82947819 Msa1000240:intron 25.0%
! ACATTACATCAAGAAACATA+AGG - chr6_4:82948191-82948210 Msa1000240:intron 25.0%
! ATATCATTTGGTAAGGTAAT+CGG + chr6_4:82948363-82948382 None:intergenic 25.0%
! AAAGGAAATATCATTTGGTA+AGG + chr6_4:82948370-82948389 None:intergenic 25.0%
! AATGGAAAGGAAATATCATT+TGG + chr6_4:82948375-82948394 None:intergenic 25.0%
! TATAATGCAGAAGATCAAAT+AGG + chr6_4:82948523-82948542 None:intergenic 25.0%
! GAATGACATCATAATAGAAA+AGG - chr6_4:82948566-82948585 Msa1000240:intron 25.0%
!! GAAAAGGCATATTTAACAAA+TGG - chr6_4:82948582-82948601 Msa1000240:intron 25.0%
! TTGATGGAGAAAAATGAAAA+TGG + chr6_4:82948751-82948770 None:intergenic 25.0%
! ACATATTTACTTCTGTTTGA+TGG + chr6_4:82948767-82948786 None:intergenic 25.0%
! ATAAAAAAAGTTGGGGAAAA+AGG + chr6_4:82948857-82948876 None:intergenic 25.0%
! TATAAAGATGGCTTATACAT+AGG - chr6_4:82949051-82949070 Msa1000240:intron 25.0%
! ATATAGTTGCTGCAATATAT+GGG + chr6_4:82949161-82949180 None:intergenic 25.0%
! TAAAACGAAAAACTACAACA+GGG - chr6_4:82949417-82949436 Msa1000240:intron 25.0%
! ATTTAGACATTTCTTCATGT+AGG - chr6_4:82949494-82949513 Msa1000240:intron 25.0%
! ATTACATCTTCAGTTCATTA+AGG - chr6_4:82949576-82949595 Msa1000240:intron 25.0%
!! AACTATCGTGTTAATTTTCA+TGG + chr6_4:82949644-82949663 None:intergenic 25.0%
! AACATGTTTACGAATATGAA+GGG + chr6_4:82950158-82950177 None:intergenic 25.0%
! TAACATGTTTACGAATATGA+AGG + chr6_4:82950159-82950178 None:intergenic 25.0%
! GTCATAAACAGACAAAAATT+AGG + chr6_4:82950223-82950242 None:intergenic 25.0%
!! GCATTTTATGAAATTATCAC+CGG + chr6_4:82950321-82950340 None:intergenic 25.0%
! TTGTGCACAAAAAGAAAATT+GGG - chr6_4:82950376-82950395 Msa1000240:intron 25.0%
!!! AATTGTTTTAATGTTCTGTC+AGG - chr6_4:82950432-82950451 Msa1000240:intron 25.0%
! TTTCCATAACTGAGTTTTCA+TGG - chr6_4:82947740-82947759 Msa1000240:intron 30.0%
TTTCAAAGTTCAAACCATGA+AGG - chr6_4:82947779-82947798 Msa1000240:intron 30.0%
!!! CAACCCTTTTTTTGATTTAG+AGG - chr6_4:82947937-82947956 Msa1000240:intron 30.0%
CAGCCTCTAAATCAAAAAAA+GGG + chr6_4:82947943-82947962 None:intergenic 30.0%
ACAGCCTCTAAATCAAAAAA+AGG + chr6_4:82947944-82947963 None:intergenic 30.0%
AAACAATCAAAAAGTGACAG+TGG - chr6_4:82948094-82948113 Msa1000240:intron 30.0%
! TCGCATTCGTATTTTACATA+TGG - chr6_4:82948251-82948270 Msa1000240:intron 30.0%
CAATAACATGCATATGTACA+TGG - chr6_4:82948332-82948351 Msa1000240:intron 30.0%
!!! TTCCCCAACTTTTTTTATGT+TGG - chr6_4:82948859-82948878 Msa1000240:intron 30.0%
!!! TCCCCAACTTTTTTTATGTT+GGG - chr6_4:82948860-82948879 Msa1000240:intron 30.0%
! ATGCTATCATTGATAGCTTA+TGG + chr6_4:82948976-82948995 None:intergenic 30.0%
GCTTATACATAGGTTATGTT+TGG - chr6_4:82949061-82949080 Msa1000240:intron 30.0%
TATAGTTGCTGCAATATATG+GGG + chr6_4:82949160-82949179 None:intergenic 30.0%
GATATAGTTGCTGCAATATA+TGG + chr6_4:82949162-82949181 None:intergenic 30.0%
!!! CGTTTTAGAACTACTTTCTT+TGG + chr6_4:82949404-82949423 None:intergenic 30.0%
CTAAAACGAAAAACTACAAC+AGG - chr6_4:82949416-82949435 Msa1000240:intron 30.0%
TTAACACGAAAACAAGATCA+AGG + chr6_4:82949475-82949494 None:intergenic 30.0%
! AAAATTGTCAGCTACAGTTT+TGG + chr6_4:82949547-82949566 None:intergenic 30.0%
TCGAATTAGTATATCACTGA+AGG - chr6_4:82949735-82949754 Msa1000240:CDS 30.0%
CGAATTAGTATATCACTGAA+GGG - chr6_4:82949736-82949755 Msa1000240:CDS 30.0%
! GATATAGGCTAATTTGAAGT+TGG - chr6_4:82949758-82949777 Msa1000240:CDS 30.0%
!! TGTCTACTTTGGGATAAATA+TGG + chr6_4:82949913-82949932 None:intergenic 30.0%
!!! ATCCCAAAGTAGACATTTTT+TGG - chr6_4:82949918-82949937 Msa1000240:intron 30.0%
CACCAAAAAATGTCTACTTT+GGG + chr6_4:82949923-82949942 None:intergenic 30.0%
TCACCAAAAAATGTCTACTT+TGG + chr6_4:82949924-82949943 None:intergenic 30.0%
GCTAAGCCAATTAGTAAATA+AGG + chr6_4:82949956-82949975 None:intergenic 30.0%
TATATACACTCTCTTCACAA+AGG - chr6_4:82950033-82950052 Msa1000240:intron 30.0%
GTTGTGCACAAAAAGAAAAT+TGG - chr6_4:82950375-82950394 Msa1000240:intron 30.0%
AGTGTAACAAGAATGCACTA+AGG + chr6_4:82947461-82947480 None:intergenic 35.0%
GGCTGTAAATTCAATTACTG+AGG - chr6_4:82947958-82947977 Msa1000240:intron 35.0%
! GATGAAAGTGTACTAGAAAG+TGG - chr6_4:82948043-82948062 Msa1000240:intron 35.0%
ATCAAAAAGTGACAGTGGAA+AGG - chr6_4:82948099-82948118 Msa1000240:intron 35.0%
TGTCTGATTCTGTAACAACA+GGG + chr6_4:82948174-82948193 None:intergenic 35.0%
ATGTCTGATTCTGTAACAAC+AGG + chr6_4:82948175-82948194 None:intergenic 35.0%
! TTCCTTTCCATTTCAAGTAG+AGG - chr6_4:82948383-82948402 Msa1000240:intron 35.0%
ACATTGTCCTCTACTTGAAA+TGG + chr6_4:82948393-82948412 None:intergenic 35.0%
! TTTCAAGTAGAGGACAATGT+TGG - chr6_4:82948393-82948412 Msa1000240:intron 35.0%
!!! TTCTTGCACTTCTTTTTCAG+TGG + chr6_4:82948653-82948672 None:intergenic 35.0%
! TTCATCTAAAGCAGTAATGC+CGG + chr6_4:82948705-82948724 None:intergenic 35.0%
TTAGATGAAGGCTAGAGTAA+AGG - chr6_4:82948716-82948735 Msa1000240:intron 35.0%
GCCCAACATAAAAAAAGTTG+GGG + chr6_4:82948864-82948883 None:intergenic 35.0%
GGCCCAACATAAAAAAAGTT+GGG + chr6_4:82948865-82948884 None:intergenic 35.0%
ATCACATAAGCCAATCCAAA+CGG + chr6_4:82948887-82948906 None:intergenic 35.0%
AGTCTCGCAAGTTATTATGT+TGG + chr6_4:82948918-82948937 None:intergenic 35.0%
! TAATAACTTGCGAGACTGTT+TGG - chr6_4:82948921-82948940 Msa1000240:intron 35.0%
ATAGTTGCTGCAATATATGG+GGG + chr6_4:82949159-82949178 None:intergenic 35.0%
!! CTCATGTGCTTTGATAATGT+CGG + chr6_4:82949265-82949284 None:intergenic 35.0%
! TTTCTTTGGTTCAGTTTCCT+TGG + chr6_4:82949390-82949409 None:intergenic 35.0%
AATTCGAATGTTTCACCGAT+CGG + chr6_4:82949722-82949741 None:intergenic 35.0%
GTATATCACTGAAGGGATAT+AGG - chr6_4:82949743-82949762 Msa1000240:CDS 35.0%
AATTAAAGTTAATGGCCCTC+TGG - chr6_4:82949862-82949881 Msa1000240:intron 35.0%
TGTTTACGAATATGAAGGGA+AGG + chr6_4:82950154-82950173 None:intergenic 35.0%
TCAAATGTCTGTGTTTGTGT+CGG + chr6_4:82950271-82950290 None:intergenic 35.0%
ACAACTAACACCTACATATG+TGG + chr6_4:82950360-82950379 None:intergenic 35.0%
AGGCCATGAAAACTCAGTTA+TGG + chr6_4:82947746-82947765 None:intergenic 40.0%
! TAACTGAGTTTTCATGGCCT+TGG - chr6_4:82947746-82947765 Msa1000240:intron 40.0%
! AACTGACTCACTTGTTGAAG+TGG - chr6_4:82948018-82948037 Msa1000240:intron 40.0%
GTCCTCTACTTGAAATGGAA+AGG + chr6_4:82948388-82948407 None:intergenic 40.0%
GCATCAATCTCTCTGTTCTT+TGG + chr6_4:82948426-82948445 None:intergenic 40.0%
! GAGATTGATGCTAGTTTGAC+TGG - chr6_4:82948435-82948454 Msa1000240:CDS 40.0%
AGACACCTGCAGTATTCTAA+CGG - chr6_4:82948674-82948693 Msa1000240:intron 40.0%
TTTGTCCGTTAGAATACTGC+AGG + chr6_4:82948682-82948701 None:intergenic 40.0%
! CAGTATTCTAACGGACAAAC+CGG - chr6_4:82948683-82948702 Msa1000240:intron 40.0%
! GGCATTACTGCTTTAGATGA+AGG - chr6_4:82948704-82948723 Msa1000240:intron 40.0%
GGGCCCAACATAAAAAAAGT+TGG + chr6_4:82948866-82948885 None:intergenic 40.0%
!!! TTTTTTATGTTGGGCCCGTT+TGG - chr6_4:82948869-82948888 Msa1000240:intron 40.0%
TCACATAAGCCAATCCAAAC+GGG + chr6_4:82948886-82948905 None:intergenic 40.0%
TAGTTGCTGCAATATATGGG+GGG + chr6_4:82949158-82949177 None:intergenic 40.0%
ACATGAGCTCCTAAGGAAAA+AGG - chr6_4:82949278-82949297 Msa1000240:intron 40.0%
!! AAATCCTGGCCTTTTTCCTT+AGG + chr6_4:82949290-82949309 None:intergenic 40.0%
TGACTTCAGTGGAAAAATCC+TGG + chr6_4:82949304-82949323 None:intergenic 40.0%
TCTTTCACTGGAGTAGTTAC+CGG + chr6_4:82949347-82949366 None:intergenic 40.0%
TGTCAACAGAAGTCCAATTG+TGG - chr6_4:82949694-82949713 Msa1000240:CDS 40.0%
ATGGGAAAAACTACACCAGA+GGG + chr6_4:82949880-82949899 None:intergenic 40.0%
AATGGGAAAAACTACACCAG+AGG + chr6_4:82949881-82949900 None:intergenic 40.0%
AATATGAAGGGAAGGTTAGG+AGG + chr6_4:82950146-82950165 None:intergenic 40.0%
ACGAATATGAAGGGAAGGTT+AGG + chr6_4:82950149-82950168 None:intergenic 40.0%
TAACACTAACATGTCGACAC+CGG - chr6_4:82950299-82950318 Msa1000240:intron 40.0%
CTGAATGCAACCACATATGT+AGG - chr6_4:82950347-82950366 Msa1000240:intron 40.0%
GATTCTGTAACAACAGGGCT+AGG + chr6_4:82948169-82948188 None:intergenic 45.0%
!! AGTGCCGATTTCTCTCATGA+GGG - chr6_4:82948462-82948481 Msa1000240:CDS 45.0%
GCTTCCCTCATGAGAGAAAT+CGG + chr6_4:82948469-82948488 None:intergenic 45.0%
CATGAGGGAAGCTTGAATAC+AGG - chr6_4:82948477-82948496 Msa1000240:CDS 45.0%
! TTTTCAGTGGCAGGACTTTC+AGG + chr6_4:82948640-82948659 None:intergenic 45.0%
!!! TGCACTTCTTTTTCAGTGGC+AGG + chr6_4:82948649-82948668 None:intergenic 45.0%
AGCACTTGTGAAGCAACTTC+AGG - chr6_4:82949185-82949204 Msa1000240:intron 45.0%
AAGCAACTTCAGGACGAAAC+AGG - chr6_4:82949195-82949214 Msa1000240:intron 45.0%
! CGAAACAGGATCTGGAATGA+TGG - chr6_4:82949209-82949228 Msa1000240:intron 45.0%
TCAAAGCACATGAGCTCCTA+AGG - chr6_4:82949271-82949290 Msa1000240:intron 45.0%
! CGGTTTTGCTGAAGTTTGTG+CGG + chr6_4:82949327-82949346 None:intergenic 45.0%
CGCACAAACTTCAGCAAAAC+CGG - chr6_4:82949325-82949344 Msa1000240:intron 45.0%
GGTAACTACTCCAGTGAAAG+AGG - chr6_4:82949346-82949365 Msa1000240:intron 45.0%
TTGGACTTCTGTTGACAACG+AGG + chr6_4:82949691-82949710 None:intergenic 45.0%
!! AGTCCAATTGTGGTACCGAT+CGG - chr6_4:82949704-82949723 Msa1000240:CDS 45.0%
!!! TTTTTTTTTTTTTTTTTATG+TGG - chr6_4:82947320-82947339 Msa1000240:intron 5.0%
TCGCTGTCTGTCTCAGTTTC+AGG + chr6_4:82948136-82948155 None:intergenic 50.0%
!! GAGTGCCGATTTCTCTCATG+AGG - chr6_4:82948461-82948480 Msa1000240:CDS 50.0%
TATGTTGGGCCCGTTTGGAT+TGG - chr6_4:82948874-82948893 Msa1000240:intron 50.0%
TTGCTGCAATATATGGGGGG+CGG + chr6_4:82949155-82949174 None:intergenic 50.0%
CTTCAGGACGAAACAGGATC+TGG - chr6_4:82949201-82949220 Msa1000240:intron 50.0%
AGCTCCTAAGGAAAAAGGCC+AGG - chr6_4:82949283-82949302 Msa1000240:intron 50.0%
AGTTTGTGCGGTGACTTCAG+TGG + chr6_4:82949315-82949334 None:intergenic 50.0%
TCACCGATCGGTACCACAAT+TGG + chr6_4:82949710-82949729 None:intergenic 50.0%
GCAGCAGGTTCCTCTTTCAC+TGG + chr6_4:82949359-82949378 None:intergenic 55.0%
ACCTGCTGCTACAGAAGCCA+AGG - chr6_4:82949370-82949389 Msa1000240:intron 55.0%
TCCTTGGCTTCTGTAGCAGC+AGG + chr6_4:82949374-82949393 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 82947282 82950469 82947282 ID=Msa1000240;Name=Msa1000240
chr6_4 mRNA 82947282 82950469 82947282 ID=Msa1000240-mRNA-1;Parent=Msa1000240;Name=Msa1000240-mRNA-1;_AED=0.49;_eAED=0.52;_QI=0|0|0|0.75|0.33|0.5|4|0|116
chr6_4 exon 82950466 82950469 82950466 ID=Msa1000240-mRNA-1:exon:16018;Parent=Msa1000240-mRNA-1
chr6_4 exon 82949577 82949794 82949577 ID=Msa1000240-mRNA-1:exon:16017;Parent=Msa1000240-mRNA-1
chr6_4 exon 82948432 82948544 82948432 ID=Msa1000240-mRNA-1:exon:16016;Parent=Msa1000240-mRNA-1
chr6_4 exon 82947282 82947297 82947282 ID=Msa1000240-mRNA-1:exon:16015;Parent=Msa1000240-mRNA-1
chr6_4 CDS 82950466 82950469 82950466 ID=Msa1000240-mRNA-1:cds;Parent=Msa1000240-mRNA-1
chr6_4 CDS 82949577 82949794 82949577 ID=Msa1000240-mRNA-1:cds;Parent=Msa1000240-mRNA-1
chr6_4 CDS 82948432 82948544 82948432 ID=Msa1000240-mRNA-1:cds;Parent=Msa1000240-mRNA-1
chr6_4 CDS 82947282 82947297 82947282 ID=Msa1000240-mRNA-1:cds;Parent=Msa1000240-mRNA-1
Gene Sequence

>Msa1000240

ATGGAGGCTGTAAATTCAATTACTGAGGAAGCTATTCAGATAGATATTGCAACAAGCAATGTCGAAACTGACTCACTTGTTGAAGTGGCTGATGAAAGTGTACTAGAAAGTGGATTTGATCAAATTGTTGCAGAAGATAAAAAACAATCAAAAAGTGACAGTGGAAAGGAAGAAGTTGCACCTGAAACTGAGACAGACAGCGATGCATTTGATCCTAGCCCTGACGAAACAGGATCTGGAATGATGGACTGCAAAAATGCTCTATCAAAGAGTCAAGCCGACATTATCAAAGCACATGAGCTCCTAAGGAAAAAGGCCAGGATTTTTCCACTGAAGAAATCAACACAGTAG

Protein sequence

>Msa1000240

MEAVNSITEEAIQIDIATSNVETDSLVEVADESVLESGFDQIVAEDKKQSKSDSGKEEVAPETETDSDAFDPSPDETGSGMMDCKNALSKSQADIIKAHELLRKKARIFPLKKSTQ*