AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1457040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1457040 MtrunA17_Chr6g0486531 63.291 158 12 2 9 120 818 975 4.39e-41 144
Msa1457040 MtrunA17_Chr6g0486531 39.623 159 42 4 15 120 577 734 2.29e-22 90.9
Msa1457040 MtrunA17_Chr6g0486321 65.686 102 19 2 35 120 34 135 4.04e-32 111
Msa1457040 MtrunA17_Chr6g0486211 81.538 65 4 1 35 91 13 77 4.95e-28 98.2
Msa1457040 MtrunA17_Chr6g0486241 81.538 65 4 1 35 91 38 102 8.77e-27 98.2
Msa1457040 MtrunA17_Chr6g0486491 58.511 94 7 2 58 119 1 94 1.78e-24 89.4
Msa1457040 MtrunA17_Chr2g0299591 80.851 47 9 0 35 81 15 61 1.93e-19 75.9
Msa1457040 MtrunA17_Chr6g0460441 43.810 105 34 3 41 120 13 117 3.36e-16 69.7
Msa1457040 MtrunA17_Chr6g0486471 45.882 85 27 2 1 66 1 85 5.07e-15 65.5
Msa1457040 MtrunA17_Chr2g0299581 49.315 73 13 1 72 120 39 111 1.24e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1457040 AT4G29060.1 51.938 129 16 2 38 120 754 882 5.06e-27 104
Msa1457040 AT4G29060.1 41.216 148 41 2 19 120 497 644 8.62e-23 92.4
Msa1457040 AT4G29060.2 43.662 142 40 2 19 120 497 638 5.33e-23 93.2

Find 63 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATGATTTGTTTGCGGATA+TGG 0.143182 tig0038321:-24911 Msa1457040:five_prime_UTR
CCTCATTGGTTAAACAATTA+AGG 0.145910 tig0038321:-21462 Msa1457040:CDS
TCGCTGTCTGTCTCAGTTTC+AGG 0.204785 tig0038321:+23890 Msa1457040:intergenic
TTCCTTCGTCACAATTGTTT+CGG 0.234648 tig0038321:+21156 Msa1457040:intergenic
TCATTAAGTTGAACTCTTGT+AGG 0.256678 tig0038321:+24848 Msa1457040:intergenic
TCCTTCGTCACAATTGTTTC+GGG 0.294632 tig0038321:+21157 Msa1457040:intergenic
TCCTTCAGCTGCTAGTCTTC+CGG 0.310289 tig0038321:+21345 Msa1457040:intergenic
TTACTTTGGTTCAGTTTCCT+TGG 0.353460 tig0038321:+22620 Msa1457040:intergenic
GACATTGCTTGTTGCAATAT+CGG 0.357465 tig0038321:+24031 Msa1457040:intergenic
TCTTTCACTGGAGTAGTTAC+CGG 0.365022 tig0038321:+22663 Msa1457040:intergenic
CTCATTGGTTAAACAATTAA+GGG 0.369404 tig0038321:-21461 Msa1457040:CDS
TGAAGAGTTGCGGGTATTGT+TGG 0.384332 tig0038321:-24885 Msa1457040:five_prime_UTR
AGTAGTTATGGTTGCGGTTG+TGG 0.392817 tig0038321:+24757 Msa1457040:intergenic
TCTTATCTGTGAAGTAGTTA+TGG 0.393868 tig0038321:+24745 Msa1457040:intergenic
ATGTCTGATTCTGTAACAAC+AGG 0.394057 tig0038321:+23851 Msa1457040:intergenic
AGTACTGGTTTCGGCCTCAT+TGG 0.405483 tig0038321:-21476 Msa1457040:CDS
AGGTCTTCTCATTGCATCTC+AGG 0.406127 tig0038321:+21127 Msa1457040:intergenic
AAGCAACTTCGGGACGAAAC+AGG 0.415386 tig0038321:-22812 Msa1457040:five_prime_UTR
TATGGTTGCGGTTGTGGTTG+TGG 0.418111 tig0038321:+24763 Msa1457040:intergenic
TGCACAAACTTCAGCAAAAC+CGG 0.423115 tig0038321:-22682 Msa1457040:intron
TCCTTGGCTTCTGTAGCAGC+AGG 0.424542 tig0038321:+22636 Msa1457040:intergenic
GAAATCAACAGTACTGGTTT+CGG 0.431645 tig0038321:-21485 Msa1457040:CDS
ATATGGAGGTGAAGAGTTGC+GGG 0.435816 tig0038321:-24894 Msa1457040:five_prime_UTR
GAAGAGTTGCGGGTATTGTT+GGG 0.436336 tig0038321:-24884 Msa1457040:five_prime_UTR
AACAGAAGGTGATCTTGAAA+AGG 0.444145 tig0038321:-21392 Msa1457040:CDS
GCAAGGTGCTGCTTGTCCTC+AGG 0.444571 tig0038321:-21206 Msa1457040:intron
AGAAACGGGTGCAGGGATGA+TGG 0.446137 tig0038321:-21437 Msa1457040:CDS
CAGGAAAGATGATTTGTTTG+CGG 0.446414 tig0038321:-24917 Msa1457040:five_prime_UTR
TAAACAATTAAGGGAAGAAA+CGG 0.450664 tig0038321:-21452 Msa1457040:CDS
TGCTGAAAATTCAATTATTG+AGG 0.450723 tig0038321:-24065 Msa1457040:five_prime_UTR
GCAGCAGGTTCCTCTTTCAC+TGG 0.453637 tig0038321:+22651 Msa1457040:intergenic
CATATCTGTGAAGCAACTTC+GGG 0.456518 tig0038321:-22822 Msa1457040:five_prime_UTR
ACATATCTGTGAAGCAACTT+CGG 0.478897 tig0038321:-22823 Msa1457040:five_prime_UTR
CGAAACAGGAGCTGGAATGA+TGG 0.479672 tig0038321:-22798 Msa1457040:exon
TTAAGGGAAGAAACGGGTGC+AGG 0.502165 tig0038321:-21445 Msa1457040:CDS
TGTCAGGAAATCAACAGTAC+TGG 0.503338 tig0038321:-21491 Msa1457040:intron
ACAGAAGGTGATCTTGAAAA+GGG 0.503619 tig0038321:-21391 Msa1457040:CDS
GATTCTGTAACAACAGGGCT+AGG 0.516573 tig0038321:+23857 Msa1457040:intergenic
CCTTAATTGTTTAACCAATG+AGG 0.525034 tig0038321:+21462 Msa1457040:intergenic
TCATCCGCTGATAAGAAATC+CGG 0.529747 tig0038321:-21364 Msa1457040:CDS
CTGTGAAGTAGTTATGGTTG+CGG 0.532069 tig0038321:+24751 Msa1457040:intergenic
GGTAACTACTCCAGTGAAAG+AGG 0.534563 tig0038321:-22661 Msa1457040:CDS
CTTCGGGACGAAACAGGAGC+TGG 0.545385 tig0038321:-22806 Msa1457040:five_prime_UTR
ATATCTTCAATAGATACAAA+CGG 0.551658 tig0038321:+21181 Msa1457040:intergenic
ACCTGCTGCTACAGAAGCCA+AGG 0.561437 tig0038321:-22637 Msa1457040:CDS
GATGAAAGTGTAATAGAAAG+TGG 0.562261 tig0038321:-23981 Msa1457040:five_prime_UTR
AAACAATTAAGGGAAGAAAC+GGG 0.568597 tig0038321:-21451 Msa1457040:CDS
ACATTACATCAAGAAACATA+AGG 0.570939 tig0038321:-23832 Msa1457040:intron
GAAGACTAGCAGCTGAAGGA+AGG 0.571916 tig0038321:-21342 Msa1457040:CDS
GATATGGAGGTGAAGAGTTG+CGG 0.571940 tig0038321:-24895 Msa1457040:five_prime_UTR
AAAGCTCTTGCTGAAACAGA+AGG 0.589113 tig0038321:-21406 Msa1457040:CDS
TCCCGAAACAATTGTGACGA+AGG 0.603689 tig0038321:-21158 Msa1457040:CDS
TCCGGAAGACTAGCAGCTGA+AGG 0.605549 tig0038321:-21346 Msa1457040:CDS
TAAGGGAAGAAACGGGTGCA+GGG 0.607891 tig0038321:-21444 Msa1457040:CDS
AATCTTGCGCTTCCAAGCGG+AGG 0.620640 tig0038321:+24809 Msa1457040:intergenic
TGATTTGTTTGCGGATATGG+AGG 0.624281 tig0038321:-24908 Msa1457040:five_prime_UTR
TCTTCCGGATTTCTTATCAG+CGG 0.628603 tig0038321:+21360 Msa1457040:intergenic
CTAAATCTTGCGCTTCCAAG+CGG 0.633412 tig0038321:+24806 Msa1457040:intergenic
TCACAGATATGTCAACAGAC+TGG 0.638706 tig0038321:+22834 Msa1457040:intergenic
AATGCTCTATCAGAGAGTCA+AGG 0.664313 tig0038321:-22767 Msa1457040:intron
ATAGATACAAACGGAACCTG+AGG 0.668908 tig0038321:+21190 Msa1457040:intergenic
TGTCTGATTCTGTAACAACA+GGG 0.689329 tig0038321:+23852 Msa1457040:intergenic
TGGATGTGATCAAATTGTTG+CGG 0.690741 tig0038321:-23961 Msa1457040:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTTCAATTAAAGTTAA+TGG - tig0038321:23989-24008 Msa1457040:five_prime_UTR 10.0%
!!! TTTTTGTTTTTGTTTTTATG+TGG - tig0038321:21394-21413 Msa1457040:CDS 15.0%
!!! TTTGAAACTAAAAATATTCA+AGG + tig0038321:21889-21908 Msa1457040:intergenic 15.0%
!!! ATTGATTTTAGTTGTAATTT+CGG + tig0038321:22322-22341 Msa1457040:intergenic 15.0%
!!! ATTGATTTTAGTTGTAATTT+CGG + tig0038321:22322-22341 Msa1457040:intergenic 15.0%
!!! ATGTAATGTATCTTTTAAAA+TGG + tig0038321:23697-23716 Msa1457040:intergenic 15.0%
!!! AAATATGGATATTTTAGAAT+GGG + tig0038321:24033-24052 Msa1457040:intergenic 15.0%
!!! TAAATATGGATATTTTAGAA+TGG + tig0038321:24034-24053 Msa1457040:intergenic 15.0%
!! TTAGTAAATAAGGATAAATA+TGG + tig0038321:24058-24077 Msa1457040:intergenic 15.0%
!!! ATTTATCCTTATTTACTAAT+TGG - tig0038321:24059-24078 Msa1457040:five_prime_UTR 15.0%
!! TATTATCTATGAATTATTCT+TGG - tig0038321:24291-24310 Msa1457040:intron 15.0%
!!! AAAGACAAACATATTTTCAT+AGG - tig0038321:21343-21362 Msa1457040:CDS 20.0%
!! TTAAGCTTACTAAATATTCA+CGG + tig0038321:21528-21547 Msa1457040:intergenic 20.0%
!! ACACATTCAATTCATATAAT+TGG + tig0038321:21799-21818 Msa1457040:intergenic 20.0%
!! AAAAACAAAAAATACCTTCA+TGG + tig0038321:21919-21938 Msa1457040:intergenic 20.0%
!! ATATCATTTGGTAAGTTAAT+CGG + tig0038321:22386-22405 Msa1457040:intergenic 20.0%
!! ATATCATTTGGTAAGTTAAT+CGG + tig0038321:22386-22405 Msa1457040:intergenic 20.0%
!!! ATTTTCTTTAACTTTGAATC+AGG - tig0038321:22725-22744 Msa1457040:intron 20.0%
!! TATAAAGATAGCTTATACAT+AGG - tig0038321:23050-23069 Msa1457040:intron 20.0%
!!! TTTGTTTTGATAAACAACTT+AGG - tig0038321:23074-23093 Msa1457040:intron 20.0%
!! TAAATAACTCAATAACAAGT+AGG + tig0038321:23470-23489 Msa1457040:intergenic 20.0%
!! GCAACTTTAAATTAAGTTAA+AGG + tig0038321:23559-23578 Msa1457040:intergenic 20.0%
!!! GATAGTTTACTATCTTTTTT+TGG + tig0038321:23963-23982 Msa1457040:intergenic 20.0%
!! AGGATAAATATGGATAAATA+TGG + tig0038321:24048-24067 Msa1457040:intergenic 20.0%
!! TTAAGTGAAAGAAAAATAGT+AGG + tig0038321:24121-24140 Msa1457040:intergenic 20.0%
!! AACATGTTTACAAATATGAA+GGG + tig0038321:24268-24287 Msa1457040:intergenic 20.0%
!! TAACATGTTTACAAATATGA+AGG + tig0038321:24269-24288 Msa1457040:intergenic 20.0%
!! ATATCTTCAATAGATACAAA+CGG + tig0038321:24871-24890 Msa1457040:intergenic 20.0%
! TAAGCTTACTAAATATTCAC+GGG + tig0038321:21527-21546 Msa1457040:intergenic 25.0%
!!! CTTTTCTAGTTTCATGATAT+CGG - tig0038321:21694-21713 Msa1457040:intron 25.0%
! TTCAATTCATATAATTGGCA+AGG + tig0038321:21794-21813 Msa1457040:intergenic 25.0%
! CAGCATCTAAATAGAAAAAA+GGG + tig0038321:21969-21988 Msa1457040:intergenic 25.0%
! TCAGCATCTAAATAGAAAAA+AGG + tig0038321:21970-21989 Msa1457040:intergenic 25.0%
! TGCTGAAAATTCAATTATTG+AGG - tig0038321:21984-22003 Msa1457040:intron 25.0%
! ACATTACATCAAGAAACATA+AGG - tig0038321:22217-22236 Msa1457040:intron 25.0%
! AATGGAAAGGAAATATCATT+TGG + tig0038321:22398-22417 Msa1457040:intergenic 25.0%
! AATGGAAAGGAAATATCATT+TGG + tig0038321:22398-22417 Msa1457040:intergenic 25.0%
! TATAATGCAGAAGATCAAAT+AGG + tig0038321:22642-22661 Msa1457040:intergenic 25.0%
! GAATGACATCATAATAGAAA+AGG - tig0038321:22685-22704 Msa1457040:intron 25.0%
!! GAAAAGGCATATTTAACAAA+TGG - tig0038321:22701-22720 Msa1457040:intron 25.0%
! ACATATTTACTTCTGTTTGA+TGG + tig0038321:22888-22907 Msa1457040:intergenic 25.0%
! ATTCATAGAACTACTTACTT+TGG + tig0038321:23446-23465 Msa1457040:intergenic 25.0%
!! CTTAATTTAAAGTTGCAAAC+AGG - tig0038321:23563-23582 Msa1457040:intron 25.0%
! TTAACACAAAAACAACATCA+AGG + tig0038321:23590-23609 Msa1457040:intergenic 25.0%
!! AACTATCGTGTTAATTTTGA+TGG + tig0038321:23778-23797 Msa1457040:intergenic 25.0%
! CGAATTAGTATATCATTGAA+TGG - tig0038321:23871-23890 Msa1457040:five_prime_UTR 25.0%
! GTATATCATTGAATGGATAT+AGG - tig0038321:23878-23897 Msa1457040:five_prime_UTR 25.0%
! GTCATAAACAGACAAAAATT+AGG + tig0038321:24333-24352 Msa1457040:intergenic 25.0%
!!! AATTGTTTTAATGTTCTGTC+AGG - tig0038321:24542-24561 Msa1457040:intron 25.0%
!! CTCATTGGTTAAACAATTAA+GGG - tig0038321:24588-24607 Msa1457040:intron 25.0%
! TAAACAATTAAGGGAAGAAA+CGG - tig0038321:24597-24616 Msa1457040:intron 25.0%
!! TCATTAAGTTGAACTCTTGT+AGG + tig0038321:21204-21223 Msa1457040:intergenic 30.0%
TCTTATCTGTGAAGTAGTTA+TGG + tig0038321:21307-21326 Msa1457040:intergenic 30.0%
! TTTCCATAACTGAGTTTTCA+TGG - tig0038321:21863-21882 Msa1457040:intron 30.0%
TTTCAAAGTTCAAACCATGA+AGG - tig0038321:21902-21921 Msa1457040:intron 30.0%
! GATGAAAGTGTAATAGAAAG+TGG - tig0038321:22068-22087 Msa1457040:intron 30.0%
AAACAATCAAAAAGTGACAG+TGG - tig0038321:22120-22139 Msa1457040:intron 30.0%
TTGCATTCGTATTTCACATA+TGG - tig0038321:22273-22292 Msa1457040:intron 30.0%
CAATAACATGCATATGTACA+TGG - tig0038321:22355-22374 Msa1457040:intron 30.0%
ATGCGATAAATGAAATGGAA+AGG + tig0038321:22411-22430 Msa1457040:intergenic 30.0%
TACGAATGCGATAAATGAAA+TGG + tig0038321:22416-22435 Msa1457040:intergenic 30.0%
CAATAACATGCATATGTACA+TGG - tig0038321:22355-22374 Msa1457040:intron 30.0%
!!! TCTTGCAATTCTTTTTTCAG+TGG + tig0038321:22773-22792 Msa1457040:intergenic 30.0%
TTGATGGAGAAAAATGAGAA+TGG + tig0038321:22872-22891 Msa1457040:intergenic 30.0%
AGTCTCACAAGTTATTATGT+TGG + tig0038321:22917-22936 Msa1457040:intergenic 30.0%
! TAATAACTTGTGAGACTGTT+TGG - tig0038321:22920-22939 Msa1457040:intron 30.0%
ATGCTATCATAGATAGCTTA+TGG + tig0038321:22975-22994 Msa1457040:intergenic 30.0%
!!! CTTTTAGAATGTAGTTTTCG+AGG + tig0038321:23517-23536 Msa1457040:intergenic 30.0%
TTTAGTCATTTCTTCATGTC+AGG - tig0038321:23610-23629 Msa1457040:intron 30.0%
! GTTTAAAACGTCAACAATGT+AGG - tig0038321:23644-23663 Msa1457040:intron 30.0%
ATTACATCTTCAGTTCATCA+AGG - tig0038321:23710-23729 Msa1457040:intron 30.0%
! GATATAGGCTAATTTGAAGT+TGG - tig0038321:23893-23912 Msa1457040:five_prime_UTR 30.0%
GCTAAGCCAATTAGTAAATA+AGG + tig0038321:24068-24087 Msa1457040:intergenic 30.0%
TATATACACTCTCTTCACAA+AGG - tig0038321:24145-24164 Msa1457040:intron 30.0%
TGTTTACAAATATGAAGGGA+AGG + tig0038321:24264-24283 Msa1457040:intergenic 30.0%
!!! GCATTTTCTGAAATTATCAC+TGG + tig0038321:24431-24450 Msa1457040:intergenic 30.0%
GTTGTGCACAAAAAGAAAAT+TGG - tig0038321:24484-24503 Msa1457040:intron 30.0%
CCTTAATTGTTTAACCAATG+AGG + tig0038321:24590-24609 Msa1457040:intergenic 30.0%
! CCTCATTGGTTAAACAATTA+AGG - tig0038321:24587-24606 Msa1457040:intron 30.0%
AAACAATTAAGGGAAGAAAC+GGG - tig0038321:24598-24617 Msa1457040:intron 30.0%
!! AGATGATTTGTTTGCGGATA+TGG - tig0038321:21138-21157 Msa1457040:CDS 35.0%
AGTGTAACAAGAATGCACTA+TGG + tig0038321:21565-21584 Msa1457040:intergenic 35.0%
! GACATTGCTTGTTGCAATAT+CGG + tig0038321:22021-22040 Msa1457040:intergenic 35.0%
TGGATGTGATCAAATTGTTG+CGG - tig0038321:22088-22107 Msa1457040:intron 35.0%
ATCAAAAAGTGACAGTGGAA+AGG - tig0038321:22125-22144 Msa1457040:intron 35.0%
TGTCTGATTCTGTAACAACA+GGG + tig0038321:22200-22219 Msa1457040:intergenic 35.0%
ATGTCTGATTCTGTAACAAC+AGG + tig0038321:22201-22220 Msa1457040:intergenic 35.0%
TCGCATTCGTATTTCACATA+TGG - tig0038321:22425-22444 Msa1457040:intron 35.0%
! TTCCTTTCCATTTCAAGTAG+AGG - tig0038321:22558-22577 Msa1457040:intron 35.0%
ACATTGTCCTCTACTTGAAA+TGG + tig0038321:22568-22587 Msa1457040:intergenic 35.0%
! TTTCAAGTAGAGGACAATGT+TGG - tig0038321:22568-22587 Msa1457040:intron 35.0%
! AATCTCTCTGTTCTTTGGAA+CGG + tig0038321:22596-22615 Msa1457040:intergenic 35.0%
!!! CAATTCTTTTTTCAGTGGCA+GGG + tig0038321:22768-22787 Msa1457040:intergenic 35.0%
TTTGTCCATTAGAATACTGC+AGG + tig0038321:22803-22822 Msa1457040:intergenic 35.0%
! CAGTATTCTAATGGACAAAC+CGG - tig0038321:22804-22823 Msa1457040:exon 35.0%
! TTCATCTAAAGCAGTAATGC+CGG + tig0038321:22826-22845 Msa1457040:intergenic 35.0%
TTAGATGAAGGCTAGAGTAA+AGG - tig0038321:22837-22856 Msa1457040:five_prime_UTR 35.0%
ACATATCTGTGAAGCAACTT+CGG - tig0038321:23226-23245 Msa1457040:intron 35.0%
!!! AAATCCTGGCTTTTTTCCTT+AGG + tig0038321:23332-23351 Msa1457040:intergenic 35.0%
! TTACTTTGGTTCAGTTTCCT+TGG + tig0038321:23432-23451 Msa1457040:intergenic 35.0%
AATTCGAATGTTTCTCCGAT+CGG + tig0038321:23857-23876 Msa1457040:intergenic 35.0%
ACAAATATGAAGGGAAGGTT+AGG + tig0038321:24259-24278 Msa1457040:intergenic 35.0%
! GACATGTGAGTGTCATTATA+TGG - tig0038321:24350-24369 Msa1457040:intron 35.0%
TCAAATGTCTGTGTTTGTGT+CGG + tig0038321:24381-24400 Msa1457040:intergenic 35.0%
CTGAATGTAACCACATATGT+AGG - tig0038321:24456-24475 Msa1457040:intron 35.0%
ACAACTAACACCTACATATG+TGG + tig0038321:24469-24488 Msa1457040:intergenic 35.0%
!! GAAATCAACAGTACTGGTTT+CGG - tig0038321:24564-24583 Msa1457040:intron 35.0%
AACAGAAGGTGATCTTGAAA+AGG - tig0038321:24657-24676 Msa1457040:intron 35.0%
ACAGAAGGTGATCTTGAAAA+GGG - tig0038321:24658-24677 Msa1457040:intron 35.0%
AAGTGAGAAATTCAAGGAGT+TGG - tig0038321:24804-24823 Msa1457040:five_prime_UTR 35.0%
TTCCTTCGTCACAATTGTTT+CGG + tig0038321:24896-24915 Msa1457040:intergenic 35.0%
!! TGATTTGTTTGCGGATATGG+AGG - tig0038321:21141-21160 Msa1457040:CDS 40.0%
CTGTGAAGTAGTTATGGTTG+CGG + tig0038321:21301-21320 Msa1457040:intergenic 40.0%
AGGCCATGAAAACTCAGTTA+TGG + tig0038321:21869-21888 Msa1457040:intergenic 40.0%
GTCCTCTACTTGAAATGGAA+AGG + tig0038321:22563-22582 Msa1457040:intergenic 40.0%
GCATCAATCTCTCTGTTCTT+TGG + tig0038321:22601-22620 Msa1457040:intergenic 40.0%
!!! GCAATTCTTTTTTCAGTGGC+AGG + tig0038321:22769-22788 Msa1457040:intergenic 40.0%
AGACACCTGCAGTATTCTAA+TGG - tig0038321:22795-22814 Msa1457040:exon 40.0%
! GGCATTACTGCTTTAGATGA+AGG - tig0038321:22825-22844 Msa1457040:five_prime_UTR 40.0%
! CGTCTGTGACGATTTTCAAT+TGG - tig0038321:23158-23177 Msa1457040:intron 40.0%
TCAATTGGATATCTTGTCCC+TGG - tig0038321:23173-23192 Msa1457040:intron 40.0%
CAATTGGATATCTTGTCCCT+GGG - tig0038321:23174-23193 Msa1457040:intron 40.0%
TCACAGATATGTCAACAGAC+TGG + tig0038321:23218-23237 Msa1457040:intergenic 40.0%
CATATCTGTGAAGCAACTTC+GGG - tig0038321:23227-23246 Msa1457040:intron 40.0%
AATGCTCTATCAGAGAGTCA+AGG - tig0038321:23282-23301 Msa1457040:intron 40.0%
TGACTTCAGCAGAAAAATCC+TGG + tig0038321:23346-23365 Msa1457040:intergenic 40.0%
TGCACAAACTTCAGCAAAAC+CGG - tig0038321:23367-23386 Msa1457040:intron 40.0%
TCTTTCACTGGAGTAGTTAC+CGG + tig0038321:23389-23408 Msa1457040:intergenic 40.0%
GTCATTTCTTCATGTCAGGT+TGG - tig0038321:23614-23633 Msa1457040:intron 40.0%
AATTAAAGTTAATGGCCCCC+TGG - tig0038321:23997-24016 Msa1457040:five_prime_UTR 40.0%
AATATGAAGGGAAGGTTAGG+AGG + tig0038321:24256-24275 Msa1457040:intergenic 40.0%
TGTCAGGAAATCAACAGTAC+TGG - tig0038321:24558-24577 Msa1457040:intron 40.0%
AAAGCTCTTGCTGAAACAGA+AGG - tig0038321:24643-24662 Msa1457040:intron 40.0%
TCATCCGCTGATAAGAAATC+CGG - tig0038321:24685-24704 Msa1457040:intron 40.0%
TCTTCCGGATTTCTTATCAG+CGG + tig0038321:24692-24711 Msa1457040:intergenic 40.0%
GTACATAGATTCACGAAGCA+CGG - tig0038321:24734-24753 Msa1457040:five_prime_UTR 40.0%
! AACTGTGAAACCGACTTTGT+TGG - tig0038321:24778-24797 Msa1457040:five_prime_UTR 40.0%
TGGCAGAAGTGAGAAATTCA+AGG - tig0038321:24798-24817 Msa1457040:five_prime_UTR 40.0%
!! GTTGATGATCTTGCAATGCA+AGG - tig0038321:24826-24845 Msa1457040:five_prime_UTR 40.0%
ATAGATACAAACGGAACCTG+AGG + tig0038321:24862-24881 Msa1457040:intergenic 40.0%
TCCTTCGTCACAATTGTTTC+GGG + tig0038321:24895-24914 Msa1457040:intergenic 40.0%
GATATGGAGGTGAAGAGTTG+CGG - tig0038321:21154-21173 Msa1457040:CDS 45.0%
ATATGGAGGTGAAGAGTTGC+GGG - tig0038321:21155-21174 Msa1457040:CDS 45.0%
! TGAAGAGTTGCGGGTATTGT+TGG - tig0038321:21164-21183 Msa1457040:CDS 45.0%
! GAAGAGTTGCGGGTATTGTT+GGG - tig0038321:21165-21184 Msa1457040:CDS 45.0%
TCTCGAAAAATTCCTCCGCT+TGG - tig0038321:21228-21247 Msa1457040:intron 45.0%
CTAAATCTTGCGCTTCCAAG+CGG + tig0038321:21246-21265 Msa1457040:intergenic 45.0%
AGTAGTTATGGTTGCGGTTG+TGG + tig0038321:21295-21314 Msa1457040:intergenic 45.0%
GATTCTGTAACAACAGGGCT+AGG + tig0038321:22195-22214 Msa1457040:intergenic 45.0%
TCGTCACAGACGATTCTGTT+TGG + tig0038321:23150-23169 Msa1457040:intergenic 45.0%
AGCTCCTAAGGAAAAAAGCC+AGG - tig0038321:23325-23344 Msa1457040:intron 45.0%
GGTAACTACTCCAGTGAAAG+AGG - tig0038321:23388-23407 Msa1457040:intron 45.0%
TTGGACTTCTGTTGACAACG+AGG + tig0038321:23826-23845 Msa1457040:intergenic 45.0%
! AGTCCAATTGTCGTACCGAT+CGG - tig0038321:23839-23858 Msa1457040:five_prime_UTR 45.0%
AATGGGCAAAACTACACCAG+GGG + tig0038321:24016-24035 Msa1457040:intergenic 45.0%
GAATGGGCAAAACTACACCA+GGG + tig0038321:24017-24036 Msa1457040:intergenic 45.0%
AGAATGGGCAAAACTACACC+AGG + tig0038321:24018-24037 Msa1457040:intergenic 45.0%
TAGATTCACGAAGCACGGAT+CGG - tig0038321:24739-24758 Msa1457040:five_prime_UTR 45.0%
CTCACTTCTGCCAACAAAGT+CGG + tig0038321:24791-24810 Msa1457040:intergenic 45.0%
TCCCGAAACAATTGTGACGA+AGG - tig0038321:24891-24910 Msa1457040:five_prime_UTR 45.0%
TATGGTTGCGGTTGTGGTTG+TGG + tig0038321:21289-21308 Msa1457040:intergenic 50.0%
TCGCTGTCTGTCTCAGTTTC+AGG + tig0038321:22162-22181 Msa1457040:intergenic 50.0%
TTTCAGTGGCAGGGACTTTC+AGG + tig0038321:22759-22778 Msa1457040:intergenic 50.0%
ATCTTGTCCCTGGGTAGAAC+TGG - tig0038321:23183-23202 Msa1457040:intron 50.0%
TCTTGTCCCTGGGTAGAACT+GGG - tig0038321:23184-23203 Msa1457040:intron 50.0%
CAAACTCCCAGTTCTACCCA+GGG + tig0038321:23193-23212 Msa1457040:intergenic 50.0%
TCAAACTCCCAGTTCTACCC+AGG + tig0038321:23194-23213 Msa1457040:intergenic 50.0%
AAGCAACTTCGGGACGAAAC+AGG - tig0038321:23237-23256 Msa1457040:intron 50.0%
CGAAACAGGAGCTGGAATGA+TGG - tig0038321:23251-23270 Msa1457040:intron 50.0%
TCAAAGCACACGAGCTCCTA+AGG - tig0038321:23313-23332 Msa1457040:intron 50.0%
TCTCCGATCGGTACGACAAT+TGG + tig0038321:23845-23864 Msa1457040:intergenic 50.0%
ATGGGCAAAACTACACCAGG+GGG + tig0038321:24015-24034 Msa1457040:intergenic 50.0%
!! AGTACTGGTTTCGGCCTCAT+TGG - tig0038321:24573-24592 Msa1457040:intron 50.0%
TTAAGGGAAGAAACGGGTGC+AGG - tig0038321:24604-24623 Msa1457040:intron 50.0%
TAAGGGAAGAAACGGGTGCA+GGG - tig0038321:24605-24624 Msa1457040:intron 50.0%
!! TCCTTCAGCTGCTAGTCTTC+CGG + tig0038321:24707-24726 Msa1457040:intergenic 50.0%
GAAGACTAGCAGCTGAAGGA+AGG - tig0038321:24707-24726 Msa1457040:five_prime_UTR 50.0%
AATCTTGCGCTTCCAAGCGG+AGG + tig0038321:21243-21262 Msa1457040:intergenic 55.0%
GCAGCAGGTTCCTCTTTCAC+TGG + tig0038321:23401-23420 Msa1457040:intergenic 55.0%
ACCTGCTGCTACAGAAGCCA+AGG - tig0038321:23412-23431 Msa1457040:intron 55.0%
TCCTTGGCTTCTGTAGCAGC+AGG + tig0038321:23416-23435 Msa1457040:intergenic 55.0%
AGAAACGGGTGCAGGGATGA+TGG - tig0038321:24612-24631 Msa1457040:intron 55.0%
TCCGGAAGACTAGCAGCTGA+AGG - tig0038321:24703-24722 Msa1457040:intron 55.0%
CTTCGGGACGAAACAGGAGC+TGG - tig0038321:23243-23262 Msa1457040:intron 60.0%
!! GCAAGGTGCTGCTTGTCCTC+AGG - tig0038321:24843-24862 Msa1457040:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
tig0038321 gene 21135 24936 21135 ID=Msa1457040;Name=Msa1457040
tig0038321 mRNA 21135 24936 21135 ID=Msa1457040-mRNA-1;Parent=Msa1457040;Name=Msa1457040-mRNA-1;_AED=0.31;_eAED=0.31;_QI=544|0.4|0.33|1|0.8|0.66|6|0|120
tig0038321 exon 21135 21223 21135 ID=Msa1457040-mRNA-1:exon:20983;Parent=Msa1457040-mRNA-1
tig0038321 exon 21338 21507 21338 ID=Msa1457040-mRNA-1:exon:20982;Parent=Msa1457040-mRNA-1
tig0038321 exon 22624 22690 22624 ID=Msa1457040-mRNA-1:exon:20981;Parent=Msa1457040-mRNA-1
tig0038321 exon 22768 22862 22768 ID=Msa1457040-mRNA-1:exon:20980;Parent=Msa1457040-mRNA-1
tig0038321 exon 23833 24088 23833 ID=Msa1457040-mRNA-1:exon:20979;Parent=Msa1457040-mRNA-1
tig0038321 exon 24707 24936 24707 ID=Msa1457040-mRNA-1:exon:20978;Parent=Msa1457040-mRNA-1
tig0038321 five_prime_UTR 24707 24936 24707 ID=Msa1457040-mRNA-1:five_prime_utr;Parent=Msa1457040-mRNA-1
tig0038321 five_prime_UTR 23833 24088 23833 ID=Msa1457040-mRNA-1:five_prime_utr;Parent=Msa1457040-mRNA-1
tig0038321 five_prime_UTR 22805 22862 22805 ID=Msa1457040-mRNA-1:five_prime_utr;Parent=Msa1457040-mRNA-1
tig0038321 CDS 22768 22804 22768 ID=Msa1457040-mRNA-1:cds;Parent=Msa1457040-mRNA-1
tig0038321 CDS 22624 22690 22624 ID=Msa1457040-mRNA-1:cds;Parent=Msa1457040-mRNA-1
tig0038321 CDS 21338 21507 21338 ID=Msa1457040-mRNA-1:cds;Parent=Msa1457040-mRNA-1
tig0038321 CDS 21135 21223 21135 ID=Msa1457040-mRNA-1:cds;Parent=Msa1457040-mRNA-1
Gene Sequence

>Msa1457040

ATGATGGACTGCAAAAATGCTCTATCAGAGAGTCAAGCAAAACCGGTAACTACTCCAGTGAAAGAGGAACCTGCTGCTACAGAAGCCAAGGAAACTGAACCAAAGAAATCAACAGTACTGGTTTCGGCCTCATTGGTTAAACAATTAAGGGAAGAAACGGGTGCAGGGATGATGGACTGTAAGAAAGCTCTTGCTGAAACAGAAGGTGATCTTGAAAAGGGTCTCTCATCCGCTGATAAGAAATCCGGAAGACTAGCAGCTGAAGGAAGGATCAGTGCTGCTTGTCCTCAGGTTCCGTTTGTATCTATTGAAGATATTCCCGAAACAATTGTGACGAAGGAAAAAGAACCTGAGATGCAATGA
Protein sequence

>Msa1457040

MMDCKNALSESQAKPVTTPVKEEPAATEAKETEPKKSTVLVSASLVKQLREETGAGMMDCKKALAETEGDLEKGLSSADKKSGRLAAEGRISAACPQVPFVSIEDIPETIVTKEKEPEMQ*