Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011810 | XP_013447886.1 | 94.161 | 137 | 7 | 1 | 1 | 136 | 1 | 137 | 1.68e-86 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011810 | A0A072TYJ6 | 94.161 | 137 | 7 | 1 | 1 | 136 | 1 | 137 | 8.03e-87 | 258 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011790 | Msa1011810 | 0.824801 | 6.628159e-54 | -8.615850e-47 |
| Msa1011810 | Msa1011820 | 0.804009 | 2.652966e-49 | -8.615850e-47 |
| Msa1011810 | Msa1011830 | 0.991339 | 3.987088e-187 | -8.615850e-47 |
| Msa1011810 | Msa1011850 | 0.875337 | 3.337328e-68 | -8.615850e-47 |
| Msa1011810 | Msa1011860 | 0.804303 | 2.304292e-49 | -8.615850e-47 |
| Msa1011810 | Msa1053290 | 0.819656 | 1.035821e-52 | -8.615850e-47 |
| Msa1011810 | Msa1053300 | 0.834390 | 3.081727e-56 | -8.615850e-47 |
| Msa1011810 | Msa1053320 | 0.855539 | 5.896467e-62 | -8.615850e-47 |
| Msa1011810 | Msa1053330 | 0.807308 | 5.386381e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011810 | MtrunA17_Chr7g0223391 | 94.161 | 137 | 7 | 1 | 1 | 136 | 18 | 154 | 2.66e-90 | 258 |
| Msa1011810 | MtrunA17_Chr7g0223401 | 90.511 | 137 | 12 | 1 | 1 | 136 | 6 | 142 | 2.64e-86 | 248 |
| Msa1011810 | MtrunA17_Chr8g0356181 | 48.571 | 140 | 68 | 2 | 1 | 136 | 1 | 140 | 1.20e-40 | 132 |
| Msa1011810 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 77 | 2 | 1 | 130 | 1 | 126 | 4.06e-19 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011810 | AT2G44310.1 | 45.985 | 137 | 71 | 2 | 1 | 134 | 1 | 137 | 2.82e-37 | 124 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_1:+16605777 | None:intergenic |
| CATTTAAGCAGCCACTTTAC+CGG | 0.324149 | 7_1:+16605581 | None:intergenic |
| CTTGAATGGCACGGTTGATC+TGG | 0.357110 | 7_1:-16605724 | Msa1011810:CDS |
| TATTAAATGCTTCATCTTCA+AGG | 0.376744 | 7_1:+16605931 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_1:-16605643 | Msa1011810:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_1:+16605639 | None:intergenic |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_1:-16605914 | Msa1011810:CDS |
| GAAGCATTTAATAGCTCAGT+TGG | 0.438228 | 7_1:-16605921 | Msa1011810:CDS |
| ATGCTTCATCTTCAAGGAAG+TGG | 0.440600 | 7_1:+16605937 | None:intergenic |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_1:+16605854 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_1:+16605778 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_1:-16605649 | Msa1011810:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_1:-16605856 | Msa1011810:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.477280 | 7_1:-16605738 | Msa1011810:CDS |
| GAAGGAGCTCAAGTGCTTGA+GGG | 0.496779 | 7_1:-16605838 | Msa1011810:CDS |
| GCTTGCAATGGCAGATGGAA+TGG | 0.514699 | 7_1:-16605670 | Msa1011810:CDS |
| ATAGCAGTTGAAAGAGAGTC+CGG | 0.521949 | 7_1:-16605600 | Msa1011810:CDS |
| CTTGCAATGGCAGATGGAAT+GGG | 0.535718 | 7_1:-16605669 | Msa1011810:CDS |
| TGAAAGAGAGTCCGGTAAAG+TGG | 0.542820 | 7_1:-16605592 | Msa1011810:CDS |
| TGAAGGAGCTCAAGTGCTTG+AGG | 0.550425 | 7_1:-16605839 | Msa1011810:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.563021 | 7_1:-16605675 | Msa1011810:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_1:-16605808 | Msa1011810:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_1:+16605947 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_1:-16605682 | Msa1011810:CDS |
| CGATCACAACTTGAATGGCA+CGG | 0.606932 | 7_1:-16605733 | Msa1011810:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_1:-16605885 | Msa1011810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGTGTAGAAATTTTAGA+TGG | - | chr7_1:16605584-16605603 | Msa1011810:CDS | 25.0% |
| ! | TATTAAATGCTTCATCTTCA+AGG | + | chr7_1:16605628-16605647 | None:intergenic | 25.0% |
| TTAATAGCTCAGTTGGAAAT+CGG | - | chr7_1:16605642-16605661 | Msa1011810:CDS | 30.0% | |
| ! | GAAGCATTTAATAGCTCAGT+TGG | - | chr7_1:16605635-16605654 | Msa1011810:CDS | 35.0% |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_1:16605737-16605756 | Msa1011810:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_1:16605748-16605767 | Msa1011810:CDS | 35.0% |
| TAAGCAAATGATGCTTGCAA+TGG | - | chr7_1:16605874-16605893 | Msa1011810:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_1:16605920-16605939 | None:intergenic | 35.0% | |
| !!! | CTCTTTCAACTGCTATTTTG+AGG | + | chr7_1:16605952-16605971 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_1:16605612-16605631 | None:intergenic | 40.0% | |
| ATGCTTCATCTTCAAGGAAG+TGG | + | chr7_1:16605622-16605641 | None:intergenic | 40.0% | |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_1:16605700-16605719 | Msa1011810:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_1:16605705-16605724 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_1:16605781-16605800 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_1:16605782-16605801 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_1:16605818-16605837 | Msa1011810:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_1:16605907-16605926 | Msa1011810:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_1:16605913-16605932 | Msa1011810:CDS | 40.0% | |
| ATAGCAGTTGAAAGAGAGTC+CGG | - | chr7_1:16605956-16605975 | Msa1011810:CDS | 40.0% | |
| TGCCTCGACACAAATAACGA+CGG | - | chr7_1:16605671-16605690 | Msa1011810:CDS | 45.0% | |
| CGATCACAACTTGAATGGCA+CGG | - | chr7_1:16605823-16605842 | Msa1011810:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_1:16605881-16605900 | Msa1011810:CDS | 45.0% | |
| CTTGCAATGGCAGATGGAAT+GGG | - | chr7_1:16605887-16605906 | Msa1011810:CDS | 45.0% | |
| TGAAAGAGAGTCCGGTAAAG+TGG | - | chr7_1:16605964-16605983 | Msa1011810:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | + | chr7_1:16605676-16605695 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGTGCTTG+AGG | - | chr7_1:16605717-16605736 | Msa1011810:CDS | 50.0% | |
| GAAGGAGCTCAAGTGCTTGA+GGG | - | chr7_1:16605718-16605737 | Msa1011810:CDS | 50.0% | |
| CTTGAATGGCACGGTTGATC+TGG | - | chr7_1:16605832-16605851 | Msa1011810:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGAA+TGG | - | chr7_1:16605886-16605905 | Msa1011810:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 16605584 | 16605994 | 16605584 | ID=Msa1011810;Name=Msa1011810 |
| chr7_1 | mRNA | 16605584 | 16605994 | 16605584 | ID=Msa1011810-mRNA-1;Parent=Msa1011810;Name=Msa1011810-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|136 |
| chr7_1 | exon | 16605584 | 16605994 | 16605584 | ID=Msa1011810-mRNA-1:exon:4227;Parent=Msa1011810-mRNA-1 |
| chr7_1 | CDS | 16605584 | 16605994 | 16605584 | ID=Msa1011810-mRNA-1:cds;Parent=Msa1011810-mRNA-1 |
| Gene Sequence |
| Protein sequence |
