Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011860 | XP_013447886.1 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 2.68e-88 | 263 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011860 | A0A072TYJ6 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 1.28e-88 | 263 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011790 | Msa1011860 | 0.906539 | 1.332447e-80 | -8.615850e-47 |
| Msa1011800 | Msa1011860 | 0.810572 | 1.078125e-50 | -8.615850e-47 |
| Msa1011810 | Msa1011860 | 0.804303 | 2.304292e-49 | -8.615850e-47 |
| Msa1011820 | Msa1011860 | 0.876353 | 1.494436e-68 | -8.615850e-47 |
| Msa1011840 | Msa1011860 | 0.877833 | 4.575955e-69 | -8.615850e-47 |
| Msa1011850 | Msa1011860 | 0.805759 | 1.142866e-49 | -8.615850e-47 |
| Msa1011860 | Msa1053290 | 0.840753 | 7.190937e-58 | -8.615850e-47 |
| Msa1011860 | Msa1053310 | 0.802370 | 5.794714e-49 | -8.615850e-47 |
| Msa1011860 | Msa1053320 | 0.800042 | 1.735291e-48 | -8.615850e-47 |
| Msa1011860 | Msa1053330 | 0.858306 | 9.016347e-63 | -8.615850e-47 |
| Msa1011860 | Msa1053340 | 0.838204 | 3.303205e-57 | -8.615850e-47 |
| Msa1011860 | Msa1095410 | 0.814363 | 1.600509e-51 | -8.615850e-47 |
| Msa1011860 | Msa1095440 | 0.818516 | 1.881836e-52 | -8.615850e-47 |
| Msa1011860 | Msa1142590 | 0.831358 | 1.746555e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011860 | MtrunA17_Chr7g0223391 | 94.891 | 137 | 7 | 0 | 1 | 137 | 18 | 154 | 5.27e-92 | 263 |
| Msa1011860 | MtrunA17_Chr7g0223401 | 89.781 | 137 | 14 | 0 | 1 | 137 | 6 | 142 | 2.07e-88 | 253 |
| Msa1011860 | MtrunA17_Chr8g0356181 | 50.000 | 140 | 67 | 1 | 1 | 137 | 1 | 140 | 6.81e-43 | 138 |
| Msa1011860 | MtrunA17_Chr1g0190921 | 35.821 | 134 | 81 | 1 | 1 | 134 | 1 | 129 | 1.12e-20 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011860 | AT2G44310.1 | 49.635 | 137 | 67 | 1 | 1 | 135 | 1 | 137 | 9.82e-42 | 135 |
Find 28 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_1:-16663498 | None:intergenic |
| CGGTTGATCTTGAAGAATTC+AGG | 0.278025 | 7_1:-16663232 | Msa1011860:CDS |
| TGAAAGAGAGTTCAGTAAAT+TGG | 0.290880 | 7_1:-16663111 | Msa1011860:CDS |
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_1:+16663296 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_1:+16663453 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_1:-16663162 | Msa1011860:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_1:+16663158 | None:intergenic |
| CAGTTTGATCACAACTTGAA+TGG | 0.425360 | 7_1:-16663257 | Msa1011860:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_1:+16663459 | None:intergenic |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.454217 | 7_1:-16663433 | Msa1011860:CDS |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_1:+16663373 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_1:+16663297 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_1:-16663168 | Msa1011860:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_1:-16663375 | Msa1011860:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.520375 | 7_1:-16663357 | Msa1011860:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_1:-16663189 | Msa1011860:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7_1:-16663440 | Msa1011860:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.545635 | 7_1:-16663194 | Msa1011860:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7_1:-16663444 | Msa1011860:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_1:-16663188 | Msa1011860:CDS |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.558625 | 7_1:-16663358 | Msa1011860:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_1:-16663327 | Msa1011860:CDS |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_1:-16663201 | Msa1011860:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.610054 | 7_1:+16663469 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.619568 | 7_1:-16663193 | Msa1011860:CDS |
| TGATCACAACTTGAATGGCA+CGG | 0.635410 | 7_1:-16663252 | Msa1011860:CDS |
| TGATCTTGAAGAATTCAGGA+AGG | 0.643588 | 7_1:-16663228 | Msa1011860:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_1:-16663404 | Msa1011860:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_1:16663103-16663122 | Msa1011860:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_1:16663147-16663166 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+CGG | - | chr7_1:16663153-16663172 | Msa1011860:CDS | 30.0% |
| TGAAAGAGAGTTCAGTAAAT+TGG | - | chr7_1:16663486-16663505 | Msa1011860:CDS | 30.0% | |
| TTAATAGCTCGGTAGGAAAT+CGG | - | chr7_1:16663164-16663183 | Msa1011860:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_1:16663259-16663278 | Msa1011860:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_1:16663270-16663289 | Msa1011860:CDS | 35.0% |
| CAGTTTGATCACAACTTGAA+TGG | - | chr7_1:16663340-16663359 | Msa1011860:CDS | 35.0% | |
| TGATCTTGAAGAATTCAGGA+AGG | - | chr7_1:16663369-16663388 | Msa1011860:CDS | 35.0% | |
| TAAGCAAATGATGCTTGCAA+TGG | - | chr7_1:16663396-16663415 | Msa1011860:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_1:16663442-16663461 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTC+AGG | + | chr7_1:16663474-16663493 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_1:16663131-16663150 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_1:16663141-16663160 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | - | chr7_1:16663157-16663176 | Msa1011860:CDS | 40.0% |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_1:16663222-16663241 | Msa1011860:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_1:16663227-16663246 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_1:16663303-16663322 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_1:16663304-16663323 | None:intergenic | 40.0% | |
| TGATCACAACTTGAATGGCA+CGG | - | chr7_1:16663345-16663364 | Msa1011860:CDS | 40.0% | |
| CGGTTGATCTTGAAGAATTC+AGG | - | chr7_1:16663365-16663384 | Msa1011860:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_1:16663429-16663448 | Msa1011860:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_1:16663435-16663454 | Msa1011860:CDS | 40.0% | |
| TGCCTCGACACAAATAACGA+CGG | - | chr7_1:16663193-16663212 | Msa1011860:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_1:16663403-16663422 | Msa1011860:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | chr7_1:16663404-16663423 | Msa1011860:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | + | chr7_1:16663198-16663217 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | - | chr7_1:16663239-16663258 | Msa1011860:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | - | chr7_1:16663240-16663259 | Msa1011860:CDS | 50.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_1:16663409-16663428 | Msa1011860:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_1:16663408-16663427 | Msa1011860:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 16663103 | 16663516 | 16663103 | ID=Msa1011860;Name=Msa1011860 |
| chr7_1 | mRNA | 16663103 | 16663516 | 16663103 | ID=Msa1011860-mRNA-1;Parent=Msa1011860;Name=Msa1011860-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_1 | exon | 16663103 | 16663516 | 16663103 | ID=Msa1011860-mRNA-1:exon:4235;Parent=Msa1011860-mRNA-1 |
| chr7_1 | CDS | 16663103 | 16663516 | 16663103 | ID=Msa1011860-mRNA-1:cds;Parent=Msa1011860-mRNA-1 |
| Gene Sequence |
| Protein sequence |
