Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011830 | XP_013447886.1 | 94.161 | 137 | 7 | 1 | 1 | 136 | 1 | 137 | 1.68e-86 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011830 | A0A072TYJ6 | 94.161 | 137 | 7 | 1 | 1 | 136 | 1 | 137 | 8.03e-87 | 258 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011790 | Msa1011830 | 0.822970 | 1.781094e-53 | -8.615850e-47 |
| Msa1011810 | Msa1011830 | 0.991339 | 3.987088e-187 | -8.615850e-47 |
| Msa1011830 | Msa1011850 | 0.877419 | 6.381238e-69 | -8.615850e-47 |
| Msa1011830 | Msa1053290 | 0.805020 | 1.632992e-49 | -8.615850e-47 |
| Msa1011830 | Msa1053300 | 0.833407 | 5.427858e-56 | -8.615850e-47 |
| Msa1011830 | Msa1053320 | 0.855577 | 5.747162e-62 | -8.615850e-47 |
| Msa1011830 | Msa1053330 | 0.800966 | 1.124849e-48 | -8.615850e-47 |
| Msa1011830 | Msa1095410 | 0.802295 | 6.003757e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011830 | MtrunA17_Chr7g0223391 | 94.161 | 137 | 7 | 1 | 1 | 136 | 18 | 154 | 2.66e-90 | 258 |
| Msa1011830 | MtrunA17_Chr7g0223401 | 90.511 | 137 | 12 | 1 | 1 | 136 | 6 | 142 | 2.64e-86 | 248 |
| Msa1011830 | MtrunA17_Chr8g0356181 | 48.571 | 140 | 68 | 2 | 1 | 136 | 1 | 140 | 1.20e-40 | 132 |
| Msa1011830 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 77 | 2 | 1 | 130 | 1 | 126 | 4.06e-19 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011830 | AT2G44310.1 | 45.985 | 137 | 71 | 2 | 1 | 134 | 1 | 137 | 2.82e-37 | 124 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_1:+16637700 | None:intergenic |
| CATTTAAGCAGCCACTTTAC+CGG | 0.324149 | 7_1:+16637504 | None:intergenic |
| CTTGAATGGCACGGTTGATC+TGG | 0.357110 | 7_1:-16637647 | Msa1011830:CDS |
| TATTAAATGCTTCATCTTCA+AGG | 0.376744 | 7_1:+16637854 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_1:-16637566 | Msa1011830:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_1:+16637562 | None:intergenic |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_1:-16637837 | Msa1011830:CDS |
| GAAGCATTTAATAGCTCAGT+TGG | 0.438228 | 7_1:-16637844 | Msa1011830:CDS |
| ATGCTTCATCTTCAAGGAAG+TGG | 0.440600 | 7_1:+16637860 | None:intergenic |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_1:+16637777 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_1:+16637701 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_1:-16637572 | Msa1011830:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_1:-16637779 | Msa1011830:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.477280 | 7_1:-16637661 | Msa1011830:CDS |
| GAAGGAGCTCAAGTGCTTGA+GGG | 0.496779 | 7_1:-16637761 | Msa1011830:CDS |
| GCTTGCAATGGCAGATGGAA+TGG | 0.514699 | 7_1:-16637593 | Msa1011830:CDS |
| ATAGCAGTTGAAAGAGAGTC+CGG | 0.521949 | 7_1:-16637523 | Msa1011830:CDS |
| CTTGCAATGGCAGATGGAAT+GGG | 0.535718 | 7_1:-16637592 | Msa1011830:CDS |
| TGAAAGAGAGTCCGGTAAAG+TGG | 0.542820 | 7_1:-16637515 | Msa1011830:CDS |
| TGAAGGAGCTCAAGTGCTTG+AGG | 0.550425 | 7_1:-16637762 | Msa1011830:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.563021 | 7_1:-16637598 | Msa1011830:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_1:-16637731 | Msa1011830:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_1:+16637870 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_1:-16637605 | Msa1011830:CDS |
| CGATCACAACTTGAATGGCA+CGG | 0.606932 | 7_1:-16637656 | Msa1011830:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_1:-16637808 | Msa1011830:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGTGTAGAAATTTTAGA+TGG | - | chr7_1:16637507-16637526 | Msa1011830:CDS | 25.0% |
| ! | TATTAAATGCTTCATCTTCA+AGG | + | chr7_1:16637551-16637570 | None:intergenic | 25.0% |
| TTAATAGCTCAGTTGGAAAT+CGG | - | chr7_1:16637565-16637584 | Msa1011830:CDS | 30.0% | |
| ! | GAAGCATTTAATAGCTCAGT+TGG | - | chr7_1:16637558-16637577 | Msa1011830:CDS | 35.0% |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_1:16637660-16637679 | Msa1011830:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_1:16637671-16637690 | Msa1011830:CDS | 35.0% |
| TAAGCAAATGATGCTTGCAA+TGG | - | chr7_1:16637797-16637816 | Msa1011830:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_1:16637843-16637862 | None:intergenic | 35.0% | |
| !!! | CTCTTTCAACTGCTATTTTG+AGG | + | chr7_1:16637875-16637894 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_1:16637535-16637554 | None:intergenic | 40.0% | |
| ATGCTTCATCTTCAAGGAAG+TGG | + | chr7_1:16637545-16637564 | None:intergenic | 40.0% | |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_1:16637623-16637642 | Msa1011830:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_1:16637628-16637647 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_1:16637704-16637723 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_1:16637705-16637724 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_1:16637741-16637760 | Msa1011830:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_1:16637830-16637849 | Msa1011830:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_1:16637836-16637855 | Msa1011830:CDS | 40.0% | |
| ATAGCAGTTGAAAGAGAGTC+CGG | - | chr7_1:16637879-16637898 | Msa1011830:CDS | 40.0% | |
| TGCCTCGACACAAATAACGA+CGG | - | chr7_1:16637594-16637613 | Msa1011830:CDS | 45.0% | |
| CGATCACAACTTGAATGGCA+CGG | - | chr7_1:16637746-16637765 | Msa1011830:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_1:16637804-16637823 | Msa1011830:CDS | 45.0% | |
| CTTGCAATGGCAGATGGAAT+GGG | - | chr7_1:16637810-16637829 | Msa1011830:CDS | 45.0% | |
| TGAAAGAGAGTCCGGTAAAG+TGG | - | chr7_1:16637887-16637906 | Msa1011830:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | + | chr7_1:16637599-16637618 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGTGCTTG+AGG | - | chr7_1:16637640-16637659 | Msa1011830:CDS | 50.0% | |
| GAAGGAGCTCAAGTGCTTGA+GGG | - | chr7_1:16637641-16637660 | Msa1011830:CDS | 50.0% | |
| CTTGAATGGCACGGTTGATC+TGG | - | chr7_1:16637755-16637774 | Msa1011830:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGAA+TGG | - | chr7_1:16637809-16637828 | Msa1011830:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 16637507 | 16637917 | 16637507 | ID=Msa1011830;Name=Msa1011830 |
| chr7_1 | mRNA | 16637507 | 16637917 | 16637507 | ID=Msa1011830-mRNA-1;Parent=Msa1011830;Name=Msa1011830-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|136 |
| chr7_1 | exon | 16637507 | 16637917 | 16637507 | ID=Msa1011830-mRNA-1:exon:4228;Parent=Msa1011830-mRNA-1 |
| chr7_1 | CDS | 16637507 | 16637917 | 16637507 | ID=Msa1011830-mRNA-1:cds;Parent=Msa1011830-mRNA-1 |
| Gene Sequence |
| Protein sequence |
