AlfalfaGEDB | Msa0943270

M. sativa cultivar zhongmu-4 / Msa0943270

KEGG: K02183
GO: GO:0005513 GO:0008150 GO:0009593 GO:0010035 GO:0010038 GO:0010099 GO:0042221 GO:0048580 GO:0048583 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051592 GO:0051606 GO:0065007 GO:2000026 GO:2000030
PFAM: PF13499.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0943270	sp\|P62201\|CALM_LILLO	100.000	149	0	0	1	149	1	149	2.36e-105	300

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0943270	A0A218X1R4	100.000	149	0	0	1	149	65	213	8.89e-104	305

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006880	Msa0943270	0.819719	1.001856e-52	-8.615850e-47
Msa0008840	Msa0943270	0.850212	1.964730e-60	-8.615850e-47
Msa0010490	Msa0943270	0.818952	1.498043e-52	-8.615850e-47
Msa0010500	Msa0943270	0.818302	2.104354e-52	-8.615850e-47
Msa0027580	Msa0943270	0.816678	4.885214e-52	-8.615850e-47
Msa0029260	Msa0943270	0.815402	9.414635e-52	-8.615850e-47
Msa0030300	Msa0943270	0.816957	4.230863e-52	-8.615850e-47
Msa0033880	Msa0943270	0.823236	1.543843e-53	-8.615850e-47
Msa0036370	Msa0943270	0.809903	1.503124e-50	-8.615850e-47
Msa0047250	Msa0943270	0.819454	1.151412e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0943270	MtrunA17_Chr6g0460211	100.000	149	0	0	1	149	1	149	1.74e-106	300
Msa0943270	MtrunA17_Chr3g0093271	99.329	149	1	0	1	149	52	200	4.50e-106	300
Msa0943270	MtrunA17_Chr5g0440931	99.329	149	1	0	1	149	1	149	6.63e-106	298
Msa0943270	MtrunA17_Chr7g0254731	98.658	149	2	0	1	149	1	149	1.79e-104	295
Msa0943270	MtrunA17_Chr1g0166281	78.378	148	32	0	1	148	1	148	2.67e-83	241
Msa0943270	MtrunA17_Chr1g0166281	47.692	65	34	0	85	149	12	76	4.12e-12	60.1
Msa0943270	MtrunA17_Chr1g0188171	77.703	148	33	0	1	148	1	148	4.41e-83	241
Msa0943270	MtrunA17_Chr1g0188171	45.070	71	36	1	82	149	6	76	2.21e-11	58.2
Msa0943270	MtrunA17_Chr7g0274101	62.838	148	55	0	1	148	1	148	1.75e-57	176
Msa0943270	MtrunA17_Chr1g0185001	58.503	147	60	1	1	147	1	146	3.46e-57	175

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0943270	AT3G43810.2	100.000	148	0	0	1	148	1	148	1.28e-106	303
Msa0943270	AT3G43810.2	45.455	77	41	1	73	149	1	76	4.87e-13	64.3
Msa0943270	AT3G43810.1	100.000	149	0	0	1	149	1	149	2.40e-106	300
Msa0943270	AT2G27030.3	99.329	149	1	0	1	149	1	149	4.05e-106	300
Msa0943270	AT2G27030.3	44.304	79	43	1	1	78	73	151	1.81e-13	64.7
Msa0943270	AT5G21274.1	99.329	149	1	0	1	149	1	149	7.60e-106	298
Msa0943270	AT3G56800.1	99.329	149	1	0	1	149	1	149	9.06e-106	298
Msa0943270	AT2G41110.1	99.329	149	1	0	1	149	1	149	9.06e-106	298
Msa0943270	AT2G27030.1	99.329	149	1	0	1	149	1	149	9.06e-106	298
Msa0943270	AT1G66410.1	97.315	149	4	0	1	149	1	149	2.46e-104	295

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Find 62 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTACGAGGAATTCGTTA+AGG	0.480590	6_3:-18896765	Msa0943270:CDS
AAGAAGAAGAAAAGCGACGA+TGG	0.585072	6_3:-18899859	Msa0943270:exon
AAGGAAATGACAATAGGGGA+GGG	0.576403	6_3:-18896670	Msa0943270:three_prime_UTR
AATGGTGAGTGAGGTTTAAG+TGG	0.506534	6_3:-18896371	Msa0943270:three_prime_UTR
ACCATTCACCAAAATATGAA+TGG	0.516525	6_3:+18896452	None:intergenic
AGCTTTCAGCTTATTCGATA+AGG	0.414465	6_3:-18899796	Msa0943270:CDS
AGGAGCTGAAAGAGGCGTTT+CGG	0.391958	6_3:-18896925	Msa0943270:CDS
AGTCGATGAGATGATCCGTG+AGG	0.760734	6_3:-18896813	Msa0943270:CDS
AGTGCTGCTGCTATTGGTTG+TGG	0.518859	6_3:-18896553	Msa0943270:three_prime_UTR
AGTTTCTACCATTCATATTT+TGG	0.145819	6_3:-18896460	Msa0943270:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCAAACAAAAACCAAATCC+TGG	+	chr6_3:18896666-18896685	None:intergenic	30.0%
ACCTATACAGTATGACAAAT+CGG	+	chr6_3:18897092-18897111	None:intergenic	30.0%
TTATTCAGTGTTGTGAATCA+CGG	-	chr6_3:18897709-18897728	Msa0943270:intron	30.0%
TCACTATTTAGTACAAAGGA+GGG	+	chr6_3:18897800-18897819	None:intergenic	30.0%
TTCACTATTTAGTACAAAGG+AGG	+	chr6_3:18897801-18897820	None:intergenic	30.0%
AATAGTGAATCACAGAACAA+TGG	-	chr6_3:18897812-18897831	Msa0943270:intron	30.0%
AACTTAGTGTTGTAAAACAG+AGG	+	chr6_3:18897869-18897888	None:intergenic	30.0%
AAAAAAAGTTGAACACACGT+TGG	-	chr6_3:18898025-18898044	Msa0943270:intron	30.0%
TTGTGGCTTATAAACTAGTA+CGG	-	chr6_3:18898056-18898075	Msa0943270:intron	30.0%
ATCAAGTAACCATCATATCA+AGG	+	chr6_3:18898114-18898133	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	18896311	18899969	18896311	ID=Msa0943270;Name=Msa0943270
chr6_3	mRNA	18896311	18899969	18896311	ID=Msa0943270-mRNA-1;Parent=Msa0943270;Name=Msa0943270-mRNA-1;_AED=0.09;_eAED=0.09;_QI=107\|1\|1\|1\|1\|1\|2\|437\|149
chr6_3	exon	18896311	18897121	18896311	ID=Msa0943270-mRNA-1:exon:5611;Parent=Msa0943270-mRNA-1
chr6_3	exon	18899787	18899969	18899787	ID=Msa0943270-mRNA-1:exon:5610;Parent=Msa0943270-mRNA-1
chr6_3	five_prime_UTR	18899863	18899969	18899863	ID=Msa0943270-mRNA-1:five_prime_utr;Parent=Msa0943270-mRNA-1
chr6_3	CDS	18899787	18899862	18899787	ID=Msa0943270-mRNA-1:cds;Parent=Msa0943270-mRNA-1
chr6_3	CDS	18896748	18897121	18896748	ID=Msa0943270-mRNA-1:cds;Parent=Msa0943270-mRNA-1
chr6_3	three_prime_UTR	18896311	18896747	18896311	ID=Msa0943270-mRNA-1:three_prime_utr;Parent=Msa0943270-mRNA-1

Gene Sequence

>Msa0943270

ATGGCAGATCAACTCACAGACGATCAGATCTCTGAGTTCAAGGAAGCTTTCAGCTTATTCGATAAGGATGGCGATGGTTGCATCACAACCAAGGAGCTTGGAACTGTTATGCGTTCATTGGGGCAAAACCCAACTGAGGCAGAGCTTCAGGACATGATCAATGAAGTAGATGCTGATGGGAATGGTACTATTGACTTCCCTGAGTTTCTTAACCTCATGGCAAGGAAGATGAAGGACACTGACTCCGAGGAGGAGCTGAAAGAGGCGTTTCGGGTTTTTGACAAGGATCAGAACGGTTTCATTTCTGCTGCTGAGCTTCGCCATGTGATGACCAACCTAGGCGAGAAGCTCACCGATGAAGAAGTCGATGAGATGATCCGTGAGGCTGATGTTGATGGTGATGGTCAAATAAACTACGAGGAATTCGTTAAGGTCATGATGGCTAAATGA

Protein sequence

>Msa0943270

MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKVMMAK*