Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1021750 | AFK40291.1 | 77.419 | 93 | 20 | 1 | 1 | 93 | 2 | 93 | 2.35e-43 | 152 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1021750 | I3SJ49 | 77.419 | 93 | 20 | 1 | 1 | 93 | 2 | 93 | 1.12e-43 | 152 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0023610 | Msa1021750 | 0.853758 | 1.933335e-61 | -8.615850e-47 |
| Msa0071340 | Msa1021750 | 0.802977 | 4.341437e-49 | -8.615850e-47 |
| Msa0675510 | Msa1021750 | 0.843427 | 1.411111e-58 | -8.615850e-47 |
| Msa0153200 | Msa1021750 | 0.832810 | 7.642586e-56 | -8.615850e-47 |
| Msa0999360 | Msa1021750 | 0.811310 | 7.464722e-51 | -8.615850e-47 |
| Msa1021740 | Msa1021750 | 0.849072 | 4.089273e-60 | -8.615850e-47 |
| Msa1021750 | Msa1105890 | 0.848843 | 4.732167e-60 | -8.615850e-47 |
| Msa1021750 | Msa1463140 | 0.819545 | 1.097655e-52 | -8.615850e-47 |
| Msa0884920 | Msa1021750 | 0.808614 | 2.839984e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1021750 | MtrunA17_Chr7g0238191 | 77.419 | 93 | 20 | 1 | 1 | 93 | 2 | 93 | 1.13e-45 | 151 |
| Msa1021750 | MtrunA17_Chr7g0238141 | 74.194 | 93 | 24 | 0 | 1 | 93 | 2 | 94 | 1.05e-41 | 141 |
| Msa1021750 | MtrunA17_Chr7g0238241 | 72.043 | 93 | 26 | 0 | 1 | 93 | 2 | 94 | 2.75e-41 | 140 |
| Msa1021750 | MtrunA17_Chr7g0238171 | 78.571 | 84 | 18 | 0 | 1 | 84 | 2 | 85 | 7.79e-40 | 137 |
| Msa1021750 | MtrunA17_Chr7g0238071 | 68.889 | 90 | 28 | 0 | 1 | 90 | 2 | 91 | 1.11e-38 | 128 |
| Msa1021750 | MtrunA17_Chr7g0238031 | 69.892 | 93 | 28 | 0 | 1 | 93 | 1 | 93 | 1.52e-38 | 134 |
| Msa1021750 | MtrunA17_Chr7g0238151 | 67.742 | 93 | 30 | 0 | 1 | 93 | 1 | 93 | 5.74e-38 | 127 |
| Msa1021750 | MtrunA17_Chr7g0238201 | 65.591 | 93 | 32 | 0 | 1 | 93 | 2 | 94 | 1.34e-37 | 124 |
| Msa1021750 | MtrunA17_Chr7g0232531 | 67.010 | 97 | 28 | 2 | 1 | 93 | 2 | 98 | 1.22e-35 | 121 |
| Msa1021750 | MtrunA17_Chr7g0238101 | 67.021 | 94 | 30 | 1 | 1 | 93 | 2 | 95 | 1.67e-35 | 125 |
| Msa1021750 | MtrunA17_Chr7g0238291 | 63.830 | 94 | 33 | 1 | 1 | 93 | 2 | 95 | 2.65e-35 | 120 |
| Msa1021750 | MtrunA17_Chr7g0238231 | 64.894 | 94 | 32 | 1 | 1 | 93 | 2 | 95 | 3.16e-35 | 124 |
| Msa1021750 | MtrunA17_Chr7g0238511 | 60.825 | 97 | 34 | 2 | 1 | 93 | 2 | 98 | 8.25e-32 | 115 |
| Msa1021750 | MtrunA17_Chr7g0238251 | 59.794 | 97 | 35 | 2 | 1 | 93 | 2 | 98 | 9.81e-29 | 107 |
| Msa1021750 | MtrunA17_Chr7g0238111 | 55.670 | 97 | 39 | 2 | 1 | 93 | 2 | 98 | 5.57e-28 | 104 |
| Msa1021750 | MtrunA17_Chr8g0352511 | 40.476 | 84 | 46 | 1 | 1 | 80 | 1 | 84 | 2.20e-15 | 69.7 |
| Msa1021750 | MtrunA17_Chr8g0354611 | 49.254 | 67 | 27 | 1 | 25 | 91 | 24 | 83 | 2.58e-15 | 68.6 |
| Msa1021750 | MtrunA17_Chr8g0354591 | 47.761 | 67 | 28 | 1 | 25 | 91 | 24 | 83 | 2.06e-14 | 67.0 |
| Msa1021750 | MtrunA17_Chr8g0354581 | 51.786 | 56 | 27 | 0 | 23 | 78 | 22 | 77 | 2.52e-14 | 66.6 |
| Msa1021750 | MtrunA17_Chr7g0238131 | 64.444 | 45 | 16 | 0 | 49 | 93 | 1 | 45 | 3.27e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCAGAAAGATACCAAATTT+TGG | 0.098652 | 7_1:-46689324 | Msa1021750:CDS |
| GTGTAGTACTTCCCAAAATT+TGG | 0.251674 | 7_1:+46689312 | None:intergenic |
| TATCTTGGGTCGTGAACTTT+CGG | 0.259672 | 7_1:+46689365 | None:intergenic |
| ATCTTGGGTCGTGAACTTTC+GGG | 0.268704 | 7_1:+46689366 | None:intergenic |
| ATATGCCACCCTTTGTATCT+TGG | 0.323049 | 7_1:+46689350 | None:intergenic |
| AGATTGATGCTAACTATTAG+AGG | 0.388438 | 7_1:-46689158 | None:intergenic |
| TATGCCACCCTTTGTATCTT+GGG | 0.401309 | 7_1:+46689351 | None:intergenic |
| GACAGAAAAGATCTGAAGTT+AGG | 0.445890 | 7_1:-46689255 | Msa1021750:CDS |
| ACACCCAAGTTGGTGTCAAT+AGG | 0.472272 | 7_1:-46689291 | Msa1021750:CDS |
| GAGAGAAGTACAAGCTTATG+TGG | 0.495648 | 7_1:-46689233 | Msa1021750:CDS |
| AGGACCTATTGACACCAACT+TGG | 0.504668 | 7_1:+46689287 | None:intergenic |
| CAAAATTTGGTATCTTTCTG+CGG | 0.547610 | 7_1:+46689325 | None:intergenic |
| TTCACGACCCAAGATACAAA+GGG | 0.583005 | 7_1:-46689358 | Msa1021750:CDS |
| ACAGAATACATAGAAAACAC+TGG | 0.600333 | 7_1:-46689207 | Msa1021750:CDS |
| GAAGTACTACACACCCAAGT+TGG | 0.600865 | 7_1:-46689301 | Msa1021750:CDS |
| GGACCTATTGACACCAACTT+GGG | 0.601545 | 7_1:+46689288 | None:intergenic |
| GTTCACGACCCAAGATACAA+AGG | 0.623259 | 7_1:-46689359 | Msa1021750:CDS |
| AGAGAAGTACAAGCTTATGT+GGG | 0.651797 | 7_1:-46689232 | Msa1021750:CDS |
| AAACACTGGACTTAGTCCAG+AGG | 0.721578 | 7_1:-46689193 | Msa1021750:CDS |
| ACGACCCAAGATACAAAGGG+TGG | 0.724861 | 7_1:-46689355 | Msa1021750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAAATTTTTTTTTAATAGTT+TGG | + | chr7_1:46689172-46689191 | None:intergenic | 10.0% |
| !! | AACTATTAAAAAAAAATTTG+CGG | - | chr7_1:46689172-46689191 | Msa1021750:CDS | 10.0% |
| CAAAATTTGGTATCTTTCTG+CGG | + | chr7_1:46689262-46689281 | None:intergenic | 30.0% | |
| ! | GCAGAAAGATACCAAATTTT+GGG | - | chr7_1:46689261-46689280 | Msa1021750:CDS | 30.0% |
| ACAGAATACATAGAAAACAC+TGG | - | chr7_1:46689377-46689396 | Msa1021750:CDS | 30.0% | |
| ! | CGCAGAAAGATACCAAATTT+TGG | - | chr7_1:46689260-46689279 | Msa1021750:CDS | 35.0% |
| GTGTAGTACTTCCCAAAATT+TGG | + | chr7_1:46689275-46689294 | None:intergenic | 35.0% | |
| !!! | TCTTTTCTGTCATGATGGAT+AGG | + | chr7_1:46689320-46689339 | None:intergenic | 35.0% |
| ! | TCAGATCTTTTCTGTCATGA+TGG | + | chr7_1:46689325-46689344 | None:intergenic | 35.0% |
| ! | GACAGAAAAGATCTGAAGTT+AGG | - | chr7_1:46689329-46689348 | Msa1021750:CDS | 35.0% |
| ! | AGAGAAGTACAAGCTTATGT+GGG | - | chr7_1:46689352-46689371 | Msa1021750:CDS | 35.0% |
| ! | ATCTTTTTGTGTAGTTCCTC+TGG | + | chr7_1:46689410-46689429 | None:intergenic | 35.0% |
| TATCTTGGGTCGTGAACTTT+CGG | + | chr7_1:46689222-46689241 | None:intergenic | 40.0% | |
| TTCACGACCCAAGATACAAA+GGG | - | chr7_1:46689226-46689245 | Msa1021750:CDS | 40.0% | |
| TATGCCACCCTTTGTATCTT+GGG | + | chr7_1:46689236-46689255 | None:intergenic | 40.0% | |
| ATATGCCACCCTTTGTATCT+TGG | + | chr7_1:46689237-46689256 | None:intergenic | 40.0% | |
| ! | GAGAGAAGTACAAGCTTATG+TGG | - | chr7_1:46689351-46689370 | Msa1021750:CDS | 40.0% |
| ATCTTGGGTCGTGAACTTTC+GGG | + | chr7_1:46689221-46689240 | None:intergenic | 45.0% | |
| GTTCACGACCCAAGATACAA+AGG | - | chr7_1:46689225-46689244 | Msa1021750:CDS | 45.0% | |
| ! | GAAGTACTACACACCCAAGT+TGG | - | chr7_1:46689283-46689302 | Msa1021750:CDS | 45.0% |
| !! | ACACCCAAGTTGGTGTCAAT+AGG | - | chr7_1:46689293-46689312 | Msa1021750:CDS | 45.0% |
| GGACCTATTGACACCAACTT+GGG | + | chr7_1:46689299-46689318 | None:intergenic | 45.0% | |
| AGGACCTATTGACACCAACT+TGG | + | chr7_1:46689300-46689319 | None:intergenic | 45.0% | |
| AAACACTGGACTTAGTCCAG+AGG | - | chr7_1:46689391-46689410 | Msa1021750:CDS | 45.0% | |
| !!! | AAATTTTTTTTTAATAGTTT+GGG | + | chr7_1:46689171-46689190 | None:intergenic | 5.0% |
| ACGACCCAAGATACAAAGGG+TGG | - | chr7_1:46689229-46689248 | Msa1021750:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 46689161 | 46689445 | 46689161 | ID=Msa1021750;Name=Msa1021750 |
| chr7_1 | mRNA | 46689161 | 46689445 | 46689161 | ID=Msa1021750-mRNA-1;Parent=Msa1021750;Name=Msa1021750-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|94 |
| chr7_1 | exon | 46689161 | 46689445 | 46689161 | ID=Msa1021750-mRNA-1:exon:9187;Parent=Msa1021750-mRNA-1 |
| chr7_1 | CDS | 46689161 | 46689445 | 46689161 | ID=Msa1021750-mRNA-1:cds;Parent=Msa1021750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
