AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0999360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0999360 MtrunA17_Chr6g0485551 57.500 80 22 1 38 117 42 109 4.56e-20 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACCATTTGATCATAAA+AGG 0.244028 6_4:-81298965 None:intergenic
TCGGACTTTCAAGTTCTTTA+CGG 0.318095 6_4:-81299203 None:intergenic
GACAGTCATTTGTGGAAGAT+TGG 0.366580 6_4:-81298715 None:intergenic
ACAGTCATTTGTGGAAGATT+GGG 0.381276 6_4:-81298714 None:intergenic
AGGCTAAAATGTGCGTGGTT+TGG 0.425751 6_4:+81299147 Msa0999360:CDS
CGGAAGCTGAAATTCGAAAG+TGG 0.452981 6_4:+81299280 Msa0999360:CDS
CTCTAAACCAGCAGTTGTTT+AGG 0.457152 6_4:+81299127 Msa0999360:intron
TTCTTCTCGACAGTCATTTG+TGG 0.465053 6_4:-81298723 None:intergenic
TGGTGATTTAAATCAGCATC+CGG 0.494036 6_4:-81299012 None:intergenic
CGAAAGTGGGCAGCTAAAGT+TGG 0.498146 6_4:+81299294 Msa0999360:CDS
AGCTAAAGTTGGAAAGCAGC+AGG 0.505164 6_4:+81299305 Msa0999360:CDS
CAGTCATTTGTGGAAGATTG+GGG 0.526783 6_4:-81298713 None:intergenic
TGGCAAGGCTCATTATAAAG+AGG 0.544099 6_4:+81299167 Msa0999360:CDS
TCGTAGTTGCATGAATAAGC+CGG 0.600958 6_4:+81298993 Msa0999360:CDS
GGATGCTGATTTAAATCACC+AGG 0.601696 6_4:+81299014 Msa0999360:CDS
CATCACATGTTACACAGACA+CGG 0.608031 6_4:+81298173 Msa0999360:CDS
AAAATGTGCGTGGTTTGGCA+AGG 0.609174 6_4:+81299152 Msa0999360:CDS
GGAAGCTGAAATTCGAAAGT+GGG 0.616430 6_4:+81299281 Msa0999360:CDS
CAAGGCTCATTATAAAGAGG+AGG 0.629971 6_4:+81299170 Msa0999360:CDS
TGTTTAGGCTAAAATGTGCG+TGG 0.663685 6_4:+81299142 Msa0999360:CDS
AGTCATTTGTGGAAGATTGG+GGG 0.693821 6_4:-81298712 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATAGAAAATTAATATTAA+AGG - chr6_4:81298923-81298942 None:intergenic 10.0%
!!! TTCTTTTTAATTTTCTTTTT+CGG - chr6_4:81299225-81299244 None:intergenic 10.0%
!! GTATAGAAAATTAATATTAA+AGG - chr6_4:81298923-81298942 None:intergenic 10.0%
!!! TTCTTTTTAATTTTCTTTTT+CGG - chr6_4:81299225-81299244 None:intergenic 10.0%
!! GAAAAAGAAAATTAAAAAGA+AGG + chr6_4:81299224-81299243 Msa0999360:CDS 15.0%
!! GAAAAAGAAAATTAAAAAGA+AGG + chr6_4:81299224-81299243 Msa0999360:CDS 15.0%
!! TTAATATTAAAGGGCTAAAA+CGG - chr6_4:81298913-81298932 None:intergenic 20.0%
!! GAAAAAGAAAATTAAGAAGA+CGG + chr6_4:81299260-81299279 Msa0999360:CDS 20.0%
!! TTAATATTAAAGGGCTAAAA+CGG - chr6_4:81298913-81298932 None:intergenic 20.0%
!! GAAAAAGAAAATTAAGAAGA+CGG + chr6_4:81299260-81299279 Msa0999360:CDS 20.0%
! GAAATCAATCATAGATTCAA+AGG - chr6_4:81298476-81298495 None:intergenic 25.0%
! GATGATAACAATCATAAAAG+AGG - chr6_4:81298631-81298650 None:intergenic 25.0%
!! TATTAATTTTCTATACCTGC+AGG + chr6_4:81298927-81298946 Msa0999360:intron 25.0%
!! TGATTCCTTTTATGATCAAA+TGG + chr6_4:81298960-81298979 Msa0999360:CDS 25.0%
! ATCAACCATTTGATCATAAA+AGG - chr6_4:81298968-81298987 None:intergenic 25.0%
! GAAATCAATCATAGATTCAA+AGG - chr6_4:81298476-81298495 None:intergenic 25.0%
! GATGATAACAATCATAAAAG+AGG - chr6_4:81298631-81298650 None:intergenic 25.0%
!! TATTAATTTTCTATACCTGC+AGG + chr6_4:81298927-81298946 Msa0999360:intron 25.0%
!! TGATTCCTTTTATGATCAAA+TGG + chr6_4:81298960-81298979 Msa0999360:CDS 25.0%
! ATCAACCATTTGATCATAAA+AGG - chr6_4:81298968-81298987 None:intergenic 25.0%
!!! GAAGATGATGATTTTTTCTG+TGG + chr6_4:81298211-81298230 Msa0999360:CDS 30.0%
!!! ATTTTTTCTGTGGAAGAAAG+TGG + chr6_4:81298221-81298240 Msa0999360:intron 30.0%
TATGATAAACCAATTCAACG+TGG + chr6_4:81298295-81298314 Msa0999360:intron 30.0%
AGGATCAAAGTGATTAAAGT+AGG + chr6_4:81298378-81298397 Msa0999360:intron 30.0%
ATTCGAATATTCTTGCATTC+AGG + chr6_4:81298507-81298526 Msa0999360:intron 30.0%
TTCGAATATTCTTGCATTCA+GGG + chr6_4:81298508-81298527 Msa0999360:intron 30.0%
! ATTTTTGATGAGAAGTTTGC+CGG - chr6_4:81298817-81298836 None:intergenic 30.0%
TCAAAAATGATAGAGATCAG+CGG + chr6_4:81298829-81298848 Msa0999360:intron 30.0%
!! TTCAAGTTCTTTACGGTTTT+CGG - chr6_4:81299199-81299218 None:intergenic 30.0%
!!! GAAGATGATGATTTTTTCTG+TGG + chr6_4:81298211-81298230 Msa0999360:CDS 30.0%
!!! ATTTTTTCTGTGGAAGAAAG+TGG + chr6_4:81298221-81298240 Msa0999360:intron 30.0%
TATGATAAACCAATTCAACG+TGG + chr6_4:81298295-81298314 Msa0999360:intron 30.0%
AGGATCAAAGTGATTAAAGT+AGG + chr6_4:81298378-81298397 Msa0999360:intron 30.0%
ATTCGAATATTCTTGCATTC+AGG + chr6_4:81298507-81298526 Msa0999360:intron 30.0%
TTCGAATATTCTTGCATTCA+GGG + chr6_4:81298508-81298527 Msa0999360:intron 30.0%
! ATTTTTGATGAGAAGTTTGC+CGG - chr6_4:81298817-81298836 None:intergenic 30.0%
TCAAAAATGATAGAGATCAG+CGG + chr6_4:81298829-81298848 Msa0999360:intron 30.0%
!! TTCAAGTTCTTTACGGTTTT+CGG - chr6_4:81299199-81299218 None:intergenic 30.0%
TCGAATATTCTTGCATTCAG+GGG + chr6_4:81298509-81298528 Msa0999360:intron 35.0%
AATGCTTCTTCTCTAGTCTA+GGG + chr6_4:81298556-81298575 Msa0999360:intron 35.0%
ACAGTCATTTGTGGAAGATT+GGG - chr6_4:81298717-81298736 None:intergenic 35.0%
AGAAATCTAAGCCAACTACT+TGG + chr6_4:81298742-81298761 Msa0999360:intron 35.0%
! CTCTTTTTGCCAGTATATGA+GGG - chr6_4:81298780-81298799 None:intergenic 35.0%
! TCTCTTTTTGCCAGTATATG+AGG - chr6_4:81298781-81298800 None:intergenic 35.0%
TATACTGGCAAAAAGAGATC+TGG + chr6_4:81298783-81298802 Msa0999360:intron 35.0%
ATACTGGCAAAAAGAGATCT+GGG + chr6_4:81298784-81298803 Msa0999360:intron 35.0%
CAAAAATGATAGAGATCAGC+GGG + chr6_4:81298830-81298849 Msa0999360:intron 35.0%
TGGTGATTTAAATCAGCATC+CGG - chr6_4:81299015-81299034 None:intergenic 35.0%
TTGTTCGAAAAAAACTTGCC+TGG - chr6_4:81299035-81299054 None:intergenic 35.0%
!! ATTTTAGCCTAAACAACTGC+TGG - chr6_4:81299137-81299156 None:intergenic 35.0%
!! TCGGACTTTCAAGTTCTTTA+CGG - chr6_4:81299206-81299225 None:intergenic 35.0%
TCGAATATTCTTGCATTCAG+GGG + chr6_4:81298509-81298528 Msa0999360:intron 35.0%
AATGCTTCTTCTCTAGTCTA+GGG + chr6_4:81298556-81298575 Msa0999360:intron 35.0%
ACAGTCATTTGTGGAAGATT+GGG - chr6_4:81298717-81298736 None:intergenic 35.0%
AGAAATCTAAGCCAACTACT+TGG + chr6_4:81298742-81298761 Msa0999360:intron 35.0%
! CTCTTTTTGCCAGTATATGA+GGG - chr6_4:81298780-81298799 None:intergenic 35.0%
! TCTCTTTTTGCCAGTATATG+AGG - chr6_4:81298781-81298800 None:intergenic 35.0%
TATACTGGCAAAAAGAGATC+TGG + chr6_4:81298783-81298802 Msa0999360:intron 35.0%
ATACTGGCAAAAAGAGATCT+GGG + chr6_4:81298784-81298803 Msa0999360:intron 35.0%
CAAAAATGATAGAGATCAGC+GGG + chr6_4:81298830-81298849 Msa0999360:intron 35.0%
TGGTGATTTAAATCAGCATC+CGG - chr6_4:81299015-81299034 None:intergenic 35.0%
TTGTTCGAAAAAAACTTGCC+TGG - chr6_4:81299035-81299054 None:intergenic 35.0%
!! ATTTTAGCCTAAACAACTGC+TGG - chr6_4:81299137-81299156 None:intergenic 35.0%
!! TCGGACTTTCAAGTTCTTTA+CGG - chr6_4:81299206-81299225 None:intergenic 35.0%
CATCACATGTTACACAGACA+CGG + chr6_4:81298173-81298192 Msa0999360:CDS 40.0%
! AGTCTGTTGCTGCAAGTTTT+GGG - chr6_4:81298259-81298278 None:intergenic 40.0%
! TAGTCTGTTGCTGCAAGTTT+TGG - chr6_4:81298260-81298279 None:intergenic 40.0%
ACTTGCAGCAACAGACTAAT+TGG + chr6_4:81298262-81298281 Msa0999360:intron 40.0%
CTTGCAGCAACAGACTAATT+GGG + chr6_4:81298263-81298282 Msa0999360:intron 40.0%
TGCAAAGATCCACGTTGAAT+TGG - chr6_4:81298307-81298326 None:intergenic 40.0%
! CTTTGGGAAAATTGTCACAG+AGG + chr6_4:81298358-81298377 Msa0999360:intron 40.0%
ATTCTTGCATTCAGGGGTTT+AGG + chr6_4:81298515-81298534 Msa0999360:intron 40.0%
TTCTTGCATTCAGGGGTTTA+GGG + chr6_4:81298516-81298535 Msa0999360:intron 40.0%
CAATGCTTCTTCTCTAGTCT+AGG + chr6_4:81298555-81298574 Msa0999360:intron 40.0%
AGTCATTTGTGGAAGATTGG+GGG - chr6_4:81298715-81298734 None:intergenic 40.0%
CAGTCATTTGTGGAAGATTG+GGG - chr6_4:81298716-81298735 None:intergenic 40.0%
GACAGTCATTTGTGGAAGAT+TGG - chr6_4:81298718-81298737 None:intergenic 40.0%
TTCTTCTCGACAGTCATTTG+TGG - chr6_4:81298726-81298745 None:intergenic 40.0%
!!! ATGAGAAGTTTGCCGGTTTT+TGG - chr6_4:81298810-81298829 None:intergenic 40.0%
TCGTAGTTGCATGAATAAGC+CGG + chr6_4:81298993-81299012 Msa0999360:CDS 40.0%
!! GGATGCTGATTTAAATCACC+AGG + chr6_4:81299014-81299033 Msa0999360:CDS 40.0%
!! CTAAACAACTGCTGGTTTAG+AGG - chr6_4:81299129-81299148 None:intergenic 40.0%
CTCTAAACCAGCAGTTGTTT+AGG + chr6_4:81299127-81299146 Msa0999360:intron 40.0%
TGTTTAGGCTAAAATGTGCG+TGG + chr6_4:81299142-81299161 Msa0999360:CDS 40.0%
TGGCAAGGCTCATTATAAAG+AGG + chr6_4:81299167-81299186 Msa0999360:CDS 40.0%
CAAGGCTCATTATAAAGAGG+AGG + chr6_4:81299170-81299189 Msa0999360:CDS 40.0%
GGAAGCTGAAATTCGAAAGT+GGG + chr6_4:81299281-81299300 Msa0999360:CDS 40.0%
CATCACATGTTACACAGACA+CGG + chr6_4:81298173-81298192 Msa0999360:CDS 40.0%
! AGTCTGTTGCTGCAAGTTTT+GGG - chr6_4:81298259-81298278 None:intergenic 40.0%
! TAGTCTGTTGCTGCAAGTTT+TGG - chr6_4:81298260-81298279 None:intergenic 40.0%
ACTTGCAGCAACAGACTAAT+TGG + chr6_4:81298262-81298281 Msa0999360:intron 40.0%
CTTGCAGCAACAGACTAATT+GGG + chr6_4:81298263-81298282 Msa0999360:intron 40.0%
TGCAAAGATCCACGTTGAAT+TGG - chr6_4:81298307-81298326 None:intergenic 40.0%
! CTTTGGGAAAATTGTCACAG+AGG + chr6_4:81298358-81298377 Msa0999360:intron 40.0%
ATTCTTGCATTCAGGGGTTT+AGG + chr6_4:81298515-81298534 Msa0999360:intron 40.0%
TTCTTGCATTCAGGGGTTTA+GGG + chr6_4:81298516-81298535 Msa0999360:intron 40.0%
CAATGCTTCTTCTCTAGTCT+AGG + chr6_4:81298555-81298574 Msa0999360:intron 40.0%
AGTCATTTGTGGAAGATTGG+GGG - chr6_4:81298715-81298734 None:intergenic 40.0%
CAGTCATTTGTGGAAGATTG+GGG - chr6_4:81298716-81298735 None:intergenic 40.0%
GACAGTCATTTGTGGAAGAT+TGG - chr6_4:81298718-81298737 None:intergenic 40.0%
TTCTTCTCGACAGTCATTTG+TGG - chr6_4:81298726-81298745 None:intergenic 40.0%
!!! ATGAGAAGTTTGCCGGTTTT+TGG - chr6_4:81298810-81298829 None:intergenic 40.0%
TCGTAGTTGCATGAATAAGC+CGG + chr6_4:81298993-81299012 Msa0999360:CDS 40.0%
!! GGATGCTGATTTAAATCACC+AGG + chr6_4:81299014-81299033 Msa0999360:CDS 40.0%
!! CTAAACAACTGCTGGTTTAG+AGG - chr6_4:81299129-81299148 None:intergenic 40.0%
CTCTAAACCAGCAGTTGTTT+AGG + chr6_4:81299127-81299146 Msa0999360:intron 40.0%
TGTTTAGGCTAAAATGTGCG+TGG + chr6_4:81299142-81299161 Msa0999360:CDS 40.0%
TGGCAAGGCTCATTATAAAG+AGG + chr6_4:81299167-81299186 Msa0999360:CDS 40.0%
CAAGGCTCATTATAAAGAGG+AGG + chr6_4:81299170-81299189 Msa0999360:CDS 40.0%
GGAAGCTGAAATTCGAAAGT+GGG + chr6_4:81299281-81299300 Msa0999360:CDS 40.0%
! GACACGGTTTTCGAATACTG+AGG + chr6_4:81298189-81298208 Msa0999360:CDS 45.0%
TTCAACGTGGATCTTTGCAG+AGG + chr6_4:81298308-81298327 Msa0999360:intron 45.0%
TCAACGTGGATCTTTGCAGA+GGG + chr6_4:81298309-81298328 Msa0999360:intron 45.0%
! TTTTCCCAAAGCGAGATGCT+GGG - chr6_4:81298349-81298368 None:intergenic 45.0%
! ATTTTCCCAAAGCGAGATGC+TGG - chr6_4:81298350-81298369 None:intergenic 45.0%
AAGAGATCTGGGCCAAAAAC+CGG + chr6_4:81298795-81298814 Msa0999360:intron 45.0%
AATCAAACAACGAAGCCTGC+AGG - chr6_4:81298945-81298964 None:intergenic 45.0%
AGGCTAAAATGTGCGTGGTT+TGG + chr6_4:81299147-81299166 Msa0999360:CDS 45.0%
! AAAATGTGCGTGGTTTGGCA+AGG + chr6_4:81299152-81299171 Msa0999360:CDS 45.0%
CGGAAGCTGAAATTCGAAAG+TGG + chr6_4:81299280-81299299 Msa0999360:CDS 45.0%
AGCTAAAGTTGGAAAGCAGC+AGG + chr6_4:81299305-81299324 Msa0999360:CDS 45.0%
! GACACGGTTTTCGAATACTG+AGG + chr6_4:81298189-81298208 Msa0999360:CDS 45.0%
TTCAACGTGGATCTTTGCAG+AGG + chr6_4:81298308-81298327 Msa0999360:intron 45.0%
TCAACGTGGATCTTTGCAGA+GGG + chr6_4:81298309-81298328 Msa0999360:intron 45.0%
! TTTTCCCAAAGCGAGATGCT+GGG - chr6_4:81298349-81298368 None:intergenic 45.0%
! ATTTTCCCAAAGCGAGATGC+TGG - chr6_4:81298350-81298369 None:intergenic 45.0%
AAGAGATCTGGGCCAAAAAC+CGG + chr6_4:81298795-81298814 Msa0999360:intron 45.0%
AATCAAACAACGAAGCCTGC+AGG - chr6_4:81298945-81298964 None:intergenic 45.0%
AGGCTAAAATGTGCGTGGTT+TGG + chr6_4:81299147-81299166 Msa0999360:CDS 45.0%
! AAAATGTGCGTGGTTTGGCA+AGG + chr6_4:81299152-81299171 Msa0999360:CDS 45.0%
CGGAAGCTGAAATTCGAAAG+TGG + chr6_4:81299280-81299299 Msa0999360:CDS 45.0%
AGCTAAAGTTGGAAAGCAGC+AGG + chr6_4:81299305-81299324 Msa0999360:CDS 45.0%
!! TATAGAAAATTAATATTAAA+GGG - chr6_4:81298922-81298941 None:intergenic 5.0%
!! TATAGAAAATTAATATTAAA+GGG - chr6_4:81298922-81298941 None:intergenic 5.0%
AGCGAGATGCTGGGACTAAT+TGG - chr6_4:81298340-81298359 None:intergenic 50.0%
TTAGTCCCAGCATCTCGCTT+TGG + chr6_4:81298341-81298360 Msa0999360:intron 50.0%
TAGTCCCAGCATCTCGCTTT+GGG + chr6_4:81298342-81298361 Msa0999360:intron 50.0%
CCTGCGATGATCCAAGTAGT+TGG - chr6_4:81298756-81298775 None:intergenic 50.0%
CCAACTACTTGGATCATCGC+AGG + chr6_4:81298753-81298772 Msa0999360:intron 50.0%
ATCGCAGGACCCTCATATAC+TGG + chr6_4:81298768-81298787 Msa0999360:intron 50.0%
CGAAAGTGGGCAGCTAAAGT+TGG + chr6_4:81299294-81299313 Msa0999360:CDS 50.0%
AGCGAGATGCTGGGACTAAT+TGG - chr6_4:81298340-81298359 None:intergenic 50.0%
TTAGTCCCAGCATCTCGCTT+TGG + chr6_4:81298341-81298360 Msa0999360:intron 50.0%
TAGTCCCAGCATCTCGCTTT+GGG + chr6_4:81298342-81298361 Msa0999360:intron 50.0%
CCTGCGATGATCCAAGTAGT+TGG - chr6_4:81298756-81298775 None:intergenic 50.0%
CCAACTACTTGGATCATCGC+AGG + chr6_4:81298753-81298772 Msa0999360:intron 50.0%
ATCGCAGGACCCTCATATAC+TGG + chr6_4:81298768-81298787 Msa0999360:intron 50.0%
CGAAAGTGGGCAGCTAAAGT+TGG + chr6_4:81299294-81299313 Msa0999360:CDS 50.0%
GGGAGAGAGTTAAGCTGCAG+AGG - chr6_4:81298695-81298714 None:intergenic 55.0%
GGGAGAGAGTTAAGCTGCAG+AGG - chr6_4:81298695-81298714 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 81298166 81299335 81298166 ID=Msa0999360;Name=Msa0999360
chr6_4 mRNA 81298166 81299335 81298166 ID=Msa0999360-mRNA-1;Parent=Msa0999360;Name=Msa0999360-mRNA-1;_AED=0.37;_eAED=0.42;_QI=0|0|0|1|0|0|4|0|142
chr6_4 exon 81298166 81298239 81298166 ID=Msa0999360-mRNA-1:exon:15540;Parent=Msa0999360-mRNA-1
chr6_4 exon 81298701 81298734 81298701 ID=Msa0999360-mRNA-1:exon:15541;Parent=Msa0999360-mRNA-1
chr6_4 exon 81298949 81299068 81298949 ID=Msa0999360-mRNA-1:exon:15542;Parent=Msa0999360-mRNA-1
chr6_4 exon 81299138 81299335 81299138 ID=Msa0999360-mRNA-1:exon:15543;Parent=Msa0999360-mRNA-1
chr6_4 CDS 81298166 81298239 81298166 ID=Msa0999360-mRNA-1:cds;Parent=Msa0999360-mRNA-1
chr6_4 CDS 81298701 81298734 81298701 ID=Msa0999360-mRNA-1:cds;Parent=Msa0999360-mRNA-1
chr6_4 CDS 81298949 81299068 81298949 ID=Msa0999360-mRNA-1:cds;Parent=Msa0999360-mRNA-1
chr6_4 CDS 81299138 81299335 81299138 ID=Msa0999360-mRNA-1:cds;Parent=Msa0999360-mRNA-1
Gene Sequence

>Msa0999360

ATGAAAACATCACATGTTACACAGACACGGTTTTCGAATACTGAGGAAGATGATGATTTTTTCTGTGGAAGAAACTTAACTCTCTCCCCCAATCTTCCACAAATGACTGCTTCGTTGTTTGATTCCTTTTATGATCAAATGGTTGATGATGTTCGTAGTTGCATGAATAAGCCGGATGCTGATTTAAATCACCAGGCAAGTTTTTTTCGAACAATGTTTTTGATTCAACAGTTGTTTAGGCTAAAATGTGCGTGGTTTGGCAAGGCTCATTATAAAGAGGAGGTTGCCGAAAACCGTAAAGAACTTGAAAGTCCGAAAAAGAAAATTAAAAAGAAGGAAGCTGAAATTCTGAAAAAGAAAATTAAGAAGACGGAAGCTGAAATTCGAAAGTGGGCAGCTAAAGTTGGAAAGCAGCAGGAGAAGAAA

Protein sequence

>Msa0999360

MKTSHVTQTRFSNTEEDDDFFCGRNLTLSPNLPQMTASLFDSFYDQMVDDVRSCMNKPDADLNHQASFFRTMFLIQQLFRLKCAWFGKAHYKEEVAENRKELESPKKKIKKKEAEILKKKIKKTEAEIRKWAAKVGKQQEKK