AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1044350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1044350 MtrunA17_Chr1g0178671 73.481 181 4 1 1 181 1 137 4.82e-89 256
Msa1044350 MtrunA17_Chr8g0383271 35.802 81 49 2 22 99 70 150 4.05e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1044350 AT2G16710.1 56.818 176 31 2 3 178 4 134 1.92e-62 189
Msa1044350 AT2G16710.3 53.846 182 33 3 3 178 4 140 4.63e-58 179
Msa1044350 AT2G16710.2 72.816 103 28 0 2 104 3 105 1.44e-50 159
Msa1044350 AT2G36260.1 48.366 153 34 2 17 169 1 108 1.34e-44 143

Find 96 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGGTATGTTCGTCATTT+TGG 0.177213 7_2:-2704474 None:intergenic
ATCATCAACGAAATCCATTT+TGG 0.208042 7_2:-2704972 None:intergenic
CCACATTGGCCTTTGGAGTT+TGG 0.248774 7_2:-2706697 None:intergenic
TCTCTATTTCTTCTATTTCA+TGG 0.257785 7_2:+2704037 Msa1044350:five_prime_UTR
AAAAGAGAAATTTGATGAAT+TGG 0.258711 7_2:+2704889 Msa1044350:CDS
TCAACCATTTCCTTGCTTAG+AGG 0.300532 7_2:-2706758 None:intergenic
TTGCACGCTGTTTGTGTTAT+GGG 0.300870 7_2:+2705267 Msa1044350:CDS
GCATGTTGTTGGAACCAAAA+TGG 0.312759 7_2:+2704958 Msa1044350:CDS
ATTGCACGCTGTTTGTGTTA+TGG 0.315018 7_2:+2705266 Msa1044350:CDS
TGCTTAGAGGCTCCAGTACT+TGG 0.316007 7_2:-2706745 None:intergenic
TTTCCAACTCATGCGCCTAA+TGG 0.325435 7_2:+2704089 Msa1044350:five_prime_UTR
TGCTGAGATTGAACGGTGTT+TGG 0.336566 7_2:-2704547 None:intergenic
GTGGATACTGTATAATTTGT+AGG 0.368896 7_2:+2707038 Msa1044350:three_prime_UTR
TCAGTACATGCCACATCATT+CGG 0.376103 7_2:+2704320 Msa1044350:five_prime_UTR
TTTAGTCATTTGAACATGTA+AGG 0.381086 7_2:-2704502 None:intergenic
TATTTCTTCTATTTCATGGT+TGG 0.382503 7_2:+2704041 Msa1044350:five_prime_UTR
TTCTTCCGAATTCGTTGGAT+TGG 0.383312 7_2:+2704432 Msa1044350:five_prime_UTR
GATGAATTGGTTGAAGATAA+AGG 0.389292 7_2:+2704902 Msa1044350:CDS
ACCACAACCACATTGGCCTT+TGG 0.397048 7_2:-2706704 None:intergenic
GCTGAGATTGAACGGTGTTT+GGG 0.419964 7_2:-2704546 None:intergenic
AGACTCCAATCCAACGAATT+CGG 0.422958 7_2:-2704437 None:intergenic
AATGACTAAATTAACCTCAT+AGG 0.422962 7_2:+2704515 Msa1044350:five_prime_UTR
GTTATGGGTCCTTTGTGTAA+TGG 0.427802 7_2:+2705282 Msa1044350:CDS
AAAATAGATGATGGTATTCA+AGG 0.433143 7_2:-2704114 None:intergenic
ATGGTATTCAAGGTTCCATT+AGG 0.437536 7_2:-2704104 None:intergenic
GAATTCGTTGGATTGGAGTC+TGG 0.444757 7_2:+2704439 Msa1044350:five_prime_UTR
TTTCGTAATTAACAAGCAAT+GGG 0.449583 7_2:+2704571 Msa1044350:exon
TAAGATATTGATTGATCCTA+AGG 0.450763 7_2:+2704928 Msa1044350:CDS
TTGGCATGCTGATACTGAAA+TGG 0.458736 7_2:+2706912 Msa1044350:three_prime_UTR
GGAGCCTCTAAGCAAGGAAA+TGG 0.461818 7_2:+2706754 Msa1044350:CDS
AAGGCTCTTATGCATGTTGT+TGG 0.463150 7_2:+2704947 Msa1044350:CDS
ATTTCGTAATTAACAAGCAA+TGG 0.463321 7_2:+2704570 Msa1044350:exon
TTTGGGGATTTATACCTATG+AGG 0.467243 7_2:-2704529 None:intergenic
CAGTACATGCCACATCATTC+GGG 0.468012 7_2:+2704321 Msa1044350:five_prime_UTR
CCACCTTCTTCCGAATTCGT+TGG 0.476252 7_2:+2704427 Msa1044350:five_prime_UTR
CGGTGCTAAATATAGCGAAC+AGG 0.479621 7_2:-2704244 None:intergenic
ACAACATGCATAAGAGCCTT+AGG 0.481683 7_2:-2704944 None:intergenic
AGGAAATTACATAGAGAAGA+AGG 0.487338 7_2:-2703993 None:intergenic
TGAACATGTAAGGACATGTA+AGG 0.491010 7_2:-2704492 None:intergenic
ATTTCGTTGATGATAAACTA+AGG 0.504621 7_2:+2704981 Msa1044350:CDS
GTCAAAGCTCGCGGATGTAA+TGG 0.505977 7_2:+2704730 Msa1044350:CDS
TTCATGACAAAACCAAGTAC+TGG 0.509644 7_2:+2706733 Msa1044350:CDS
GGGTTCCAAGGCGATGGCTA+TGG 0.513236 7_2:+2704591 Msa1044350:CDS
TCCAAAGGCCAATGTGGTTG+TGG 0.524509 7_2:+2706703 Msa1044350:CDS
CCTCGCGAAATCACGAAATA+CGG 0.525892 7_2:-2704218 None:intergenic
CATATAGGTTGCATTTCACG+TGG 0.529348 7_2:+2705181 Msa1044350:intron
AATCCAACGAATTCGGAAGA+AGG 0.529950 7_2:-2704430 None:intergenic
TTCATTAATCCAAACTCCAA+AGG 0.536589 7_2:+2706688 Msa1044350:CDS
TGCTGCCATAGCCATCGCCT+TGG 0.537499 7_2:-2704596 None:intergenic
GTTGTCAGAAGAAGATCTAG+AGG 0.539767 7_2:-2704013 None:intergenic
ACGAAATTGCTGAGATTGAA+CGG 0.542457 7_2:-2704554 None:intergenic
AGGTGTTGTTGACTTGTCGT+AGG 0.551502 7_2:-2703970 None:intergenic
TATTCTAGAGGCGGCGGCAT+CGG 0.552495 7_2:-2704662 None:intergenic
GTTCCATTAGGCGCATGAGT+TGG 0.558660 7_2:-2704092 None:intergenic
CAAAGACCGTTCTTAAAGCT+CGG 0.558956 7_2:+2704706 Msa1044350:CDS
TTAACAAGCAATGGGTTCCA+AGG 0.561441 7_2:+2704579 Msa1044350:exon
TACACTTCTTGCTTCAGCAA+CGG 0.562185 7_2:+2704683 Msa1044350:CDS
GTTGAAATTATCATTCAACT+AGG 0.573251 7_2:+2706776 Msa1044350:exon
AGCAATGGGTTCCAAGGCGA+TGG 0.573401 7_2:+2704585 Msa1044350:exon
AGATAAGCACGGCGGTGCGA+CGG 0.576266 7_2:+2704623 Msa1044350:CDS
AATTGTTGTGCGTATGGCAG+TGG 0.579932 7_2:-2704403 None:intergenic
GGTGGAAATTGTTGTGCGTA+TGG 0.580291 7_2:-2704409 None:intergenic
GTAATGGACCACTCTTCCAC+AGG 0.585685 7_2:+2705298 Msa1044350:CDS
GGCGGCATCGGTTAACTCAA+CGG 0.586444 7_2:-2704650 None:intergenic
TCTGTGGCTATGAATTTCAG+AGG 0.588281 7_2:+2704136 Msa1044350:five_prime_UTR
CTCTGAAGCTGATACTGACA+AGG 0.590200 7_2:+2706952 Msa1044350:three_prime_UTR
TAGCCACAGAAAATAGATGA+TGG 0.591925 7_2:-2704123 None:intergenic
CCAACGAATTCGGAAGAAGG+TGG 0.596750 7_2:-2704427 None:intergenic
AAATTACATAGAGAAGAAGG+AGG 0.601528 7_2:-2703990 None:intergenic
GAAGTGTATTCTAGAGGCGG+CGG 0.606928 7_2:-2704668 None:intergenic
TTTAATTTAATGAGAAAGAG+CGG 0.607923 7_2:-2704264 None:intergenic
TTGTCGTAGGCTTATAGCGG+TGG 0.610408 7_2:-2703957 None:intergenic
CTGAGATTGAACGGTGTTTG+GGG 0.614313 7_2:-2704545 None:intergenic
AAATTACAGAAATTCAATTG+TGG 0.623213 7_2:-2706857 None:intergenic
AGTACTGGAGCCTCTAAGCA+AGG 0.624921 7_2:+2706748 Msa1044350:CDS
AAGATTCACCACAACCACAT+TGG 0.628045 7_2:-2706711 None:intergenic
CCAAACTCCAAAGGCCAATG+TGG 0.629156 7_2:+2706697 Msa1044350:CDS
GACTTGTCGTAGGCTTATAG+CGG 0.631925 7_2:-2703960 None:intergenic
CCGTATTTCGTGATTTCGCG+AGG 0.633478 7_2:+2704218 Msa1044350:five_prime_UTR
GAACATGTAAGGACATGTAA+GGG 0.635484 7_2:-2704491 None:intergenic
CGAAAACGTCCCGAATGATG+TGG 0.636787 7_2:-2704330 None:intergenic
CAAGAAGTGTATTCTAGAGG+CGG 0.641303 7_2:-2704671 None:intergenic
TGGTATTTGCATGTTCACCA+TGG 0.645858 7_2:-2706837 None:intergenic
AAGCAAGAAGTGTATTCTAG+AGG 0.646366 7_2:-2704674 None:intergenic
GGCAGCAGAGAAGATAAGCA+CGG 0.647942 7_2:+2704612 Msa1044350:CDS
TTGACGCCGAGCTTTAAGAA+CGG 0.652738 7_2:-2704712 None:intergenic
AAGCTCGGCGTCAAAGCTCG+CGG 0.657327 7_2:+2704721 Msa1044350:CDS
TCTTACACGCTAAATTACGC+AGG 0.659336 7_2:+2704757 Msa1044350:CDS
TCACGTGGGTGACTGTGCTG+AGG 0.673939 7_2:+2705196 Msa1044350:CDS
TTACGGAACCTGTGGAAGAG+TGG 0.674207 7_2:-2705306 None:intergenic
GTTTGAAAACATGAGTGTGG+TGG 0.682217 7_2:-2704191 None:intergenic
ATATAGGTTGCATTTCACGT+GGG 0.699722 7_2:+2705182 Msa1044350:intron
TCAGTTTGAAAACATGAGTG+TGG 0.728519 7_2:-2704194 None:intergenic
AGCAGAGAAGATAAGCACGG+CGG 0.734036 7_2:+2704615 Msa1044350:CDS
AAGAGTGGTCCATTACACAA+AGG 0.734584 7_2:-2705291 None:intergenic
TTATTGAAGAGAGAATCGAA+AGG 0.762981 7_2:-2704164 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATTAAAATTAAAAA+AGG + chr7_2:2704281-2704300 Msa1044350:five_prime_UTR 0.0%
!! ATTAAACGAAAATTTAAAGA+GGG - chr7_2:2704796-2704815 None:intergenic 15.0%
!! AATTAAACGAAAATTTAAAG+AGG - chr7_2:2704797-2704816 None:intergenic 15.0%
!!! TTTAATTTAATGAGAAAGAG+CGG - chr7_2:2704267-2704286 None:intergenic 20.0%
!!! GTATTGATTTTAAGATTTGT+TGG + chr7_2:2704848-2704867 Msa1044350:intron 20.0%
!! AAAAGAGAAATTTGATGAAT+TGG + chr7_2:2704889-2704908 Msa1044350:CDS 20.0%
!!! TTCTTTGTTCAATTTTGTTT+TGG + chr7_2:2705042-2705061 Msa1044350:intron 20.0%
!!! TTTTTTTTCCCCAATAAATA+AGG - chr7_2:2705081-2705100 None:intergenic 20.0%
!! TAATTATATGATGAGGTTTA+TGG - chr7_2:2705602-2705621 None:intergenic 20.0%
!!! TTGCTTATGTTTTTTCTTTT+CGG + chr7_2:2706614-2706633 Msa1044350:intron 20.0%
!! AAATTACAGAAATTCAATTG+TGG - chr7_2:2706860-2706879 None:intergenic 20.0%
!! ATTCATGTAAAAAACTATTG+TGG + chr7_2:2707019-2707038 Msa1044350:three_prime_UTR 20.0%
!! TCTCTATTTCTTCTATTTCA+TGG + chr7_2:2704037-2704056 Msa1044350:five_prime_UTR 25.0%
! TATTTCTTCTATTTCATGGT+TGG + chr7_2:2704041-2704060 Msa1044350:five_prime_UTR 25.0%
!! AAAATAGATGATGGTATTCA+AGG - chr7_2:2704117-2704136 None:intergenic 25.0%
! TTAAAAAAGGAAAATGCAAG+TGG + chr7_2:2704294-2704313 Msa1044350:five_prime_UTR 25.0%
! TAAAAAAGGAAAATGCAAGT+GGG + chr7_2:2704295-2704314 Msa1044350:five_prime_UTR 25.0%
! TTTAGTCATTTGAACATGTA+AGG - chr7_2:2704505-2704524 None:intergenic 25.0%
! AATGACTAAATTAACCTCAT+AGG + chr7_2:2704515-2704534 Msa1044350:five_prime_UTR 25.0%
! ATTTCGTAATTAACAAGCAA+TGG + chr7_2:2704570-2704589 Msa1044350:exon 25.0%
! TTTCGTAATTAACAAGCAAT+GGG + chr7_2:2704571-2704590 Msa1044350:exon 25.0%
!!! TTGATTTTAAGATTTGTTGG+TGG + chr7_2:2704851-2704870 Msa1044350:intron 25.0%
!! TAAGATATTGATTGATCCTA+AGG + chr7_2:2704928-2704947 Msa1044350:CDS 25.0%
!!! ATTTCGTTGATGATAAACTA+AGG + chr7_2:2704981-2705000 Msa1044350:CDS 25.0%
!! TCTTGTTCAACCTTATTTAT+TGG + chr7_2:2705068-2705087 Msa1044350:intron 25.0%
!! CTTGTTCAACCTTATTTATT+GGG + chr7_2:2705069-2705088 Msa1044350:intron 25.0%
!! TTGTTCAACCTTATTTATTG+GGG + chr7_2:2705070-2705089 Msa1044350:intron 25.0%
! AAAAAAACGCAAACATTGAT+TGG + chr7_2:2705094-2705113 Msa1044350:intron 25.0%
!! AAAAAACGCAAACATTGATT+GGG + chr7_2:2705095-2705114 Msa1044350:intron 25.0%
! AGTATGTCATAAATCTCATT+TGG + chr7_2:2705799-2705818 Msa1044350:intron 25.0%
! AAGTAATGCATATTCCTAAT+TGG - chr7_2:2705903-2705922 None:intergenic 25.0%
! TAGGAATATGCATTACTTAA+AGG + chr7_2:2705905-2705924 Msa1044350:intron 25.0%
! AGGAATATGCATTACTTAAA+GGG + chr7_2:2705906-2705925 Msa1044350:intron 25.0%
! GTTGAAATTATCATTCAACT+AGG + chr7_2:2706776-2706795 Msa1044350:exon 25.0%
!! TCTTTCTTTTATGATTTCCA+TGG + chr7_2:2706820-2706839 Msa1044350:three_prime_UTR 25.0%
! AGAGGAAAATAAAAAAGCTT+TGG + chr7_2:2706893-2706912 Msa1044350:three_prime_UTR 25.0%
AAATTACATAGAGAAGAAGG+AGG - chr7_2:2703993-2704012 None:intergenic 30.0%
AGGAAATTACATAGAGAAGA+AGG - chr7_2:2703996-2704015 None:intergenic 30.0%
! TGAGTTGGAAAGAAAAACTA+TGG - chr7_2:2704080-2704099 None:intergenic 30.0%
! ATACCATCATCTATTTTCTG+TGG + chr7_2:2704120-2704139 Msa1044350:five_prime_UTR 30.0%
TTATTGAAGAGAGAATCGAA+AGG - chr7_2:2704167-2704186 None:intergenic 30.0%
GATGAATTGGTTGAAGATAA+AGG + chr7_2:2704902-2704921 Msa1044350:CDS 30.0%
! ATCATCAACGAAATCCATTT+TGG - chr7_2:2704975-2704994 None:intergenic 30.0%
! AAAAACGCAAACATTGATTG+GGG + chr7_2:2705096-2705115 Msa1044350:intron 30.0%
TCAGTAAACCTTACTAGTTA+AGG - chr7_2:2705147-2705166 None:intergenic 30.0%
ATTATGTAATGAACCAGTTG+AGG - chr7_2:2705424-2705443 None:intergenic 30.0%
! TTACATAATTTGCATGCTAC+AGG + chr7_2:2705435-2705454 Msa1044350:intron 30.0%
! TACATAATTTGCATGCTACA+GGG + chr7_2:2705436-2705455 Msa1044350:intron 30.0%
CCTCATCATATAATTATACC+CGG + chr7_2:2705606-2705625 Msa1044350:intron 30.0%
! TATTTTACCCACGTAGAATT+GGG - chr7_2:2706026-2706045 None:intergenic 30.0%
! TACATCAGTGTTGTTAATTG+CGG + chr7_2:2706125-2706144 Msa1044350:intron 30.0%
ATTAGTTACTGCTTGATCTT+TGG + chr7_2:2706245-2706264 Msa1044350:intron 30.0%
AATATGCTACAATGCTATAG+CGG + chr7_2:2706312-2706331 Msa1044350:intron 30.0%
TTGTACTAAATAGCATACCA+CGG + chr7_2:2706359-2706378 Msa1044350:intron 30.0%
! GTCTAAAGCTTTATGTACAA+GGG - chr7_2:2706514-2706533 None:intergenic 30.0%
AAAAAACATAAGCAAGTAGC+AGG - chr7_2:2706609-2706628 None:intergenic 30.0%
! AAATACTATTTCTCCTCTTC+TGG + chr7_2:2706649-2706668 Msa1044350:intron 30.0%
TTCATTAATCCAAACTCCAA+AGG + chr7_2:2706688-2706707 Msa1044350:CDS 30.0%
! GTGGATACTGTATAATTTGT+AGG + chr7_2:2707038-2707057 Msa1044350:three_prime_UTR 30.0%
ATGGTATTCAAGGTTCCATT+AGG - chr7_2:2704107-2704126 None:intergenic 35.0%
TAGCCACAGAAAATAGATGA+TGG - chr7_2:2704126-2704145 None:intergenic 35.0%
TCAGTTTGAAAACATGAGTG+TGG - chr7_2:2704197-2704216 None:intergenic 35.0%
!! TAAGGGTATGTTCGTCATTT+TGG - chr7_2:2704477-2704496 None:intergenic 35.0%
GAACATGTAAGGACATGTAA+GGG - chr7_2:2704494-2704513 None:intergenic 35.0%
TGAACATGTAAGGACATGTA+AGG - chr7_2:2704495-2704514 None:intergenic 35.0%
TTTGGGGATTTATACCTATG+AGG - chr7_2:2704532-2704551 None:intergenic 35.0%
ACGAAATTGCTGAGATTGAA+CGG - chr7_2:2704557-2704576 None:intergenic 35.0%
! AAGCAAGAAGTGTATTCTAG+AGG - chr7_2:2704677-2704696 None:intergenic 35.0%
! AAAACGCAAACATTGATTGG+GGG + chr7_2:2705097-2705116 Msa1044350:intron 35.0%
ACTAGTTAAGGCTTCAAACA+TGG - chr7_2:2705135-2705154 None:intergenic 35.0%
! GTTTGAAGCCTTAACTAGTA+AGG + chr7_2:2705136-2705155 Msa1044350:intron 35.0%
ATATAGGTTGCATTTCACGT+GGG + chr7_2:2705182-2705201 Msa1044350:intron 35.0%
ATTGCACGCTATTTGTGTTA+CGG - chr7_2:2705326-2705345 None:intergenic 35.0%
AGTTCTATGTTGAAGGAGAT+GGG - chr7_2:2705571-2705590 None:intergenic 35.0%
AAGTTCTATGTTGAAGGAGA+TGG - chr7_2:2705572-2705591 None:intergenic 35.0%
CCGGGTATAATTATATGATG+AGG - chr7_2:2705609-2705628 None:intergenic 35.0%
! GTATTTATTCGGTTTGAACC+GGG - chr7_2:2705627-2705646 None:intergenic 35.0%
! GGTATTTATTCGGTTTGAAC+CGG - chr7_2:2705628-2705647 None:intergenic 35.0%
CGAGAGGAATGGTATTTATT+CGG - chr7_2:2705638-2705657 None:intergenic 35.0%
AAACTATCCACCTCAGTATT+AGG + chr7_2:2705670-2705689 Msa1044350:intron 35.0%
!! ACTTTTTCCTAATACTGAGG+TGG - chr7_2:2705680-2705699 None:intergenic 35.0%
ACCTCAGTATTAGGAAAAAG+TGG + chr7_2:2705679-2705698 Msa1044350:intron 35.0%
CCTCAGTATTAGGAAAAAGT+GGG + chr7_2:2705680-2705699 Msa1044350:intron 35.0%
! TGTTGTCCAGCATATTTTGT+CGG - chr7_2:2705707-2705726 None:intergenic 35.0%
TGCATATTCCTAATTGGTGA+TGG - chr7_2:2705897-2705916 None:intergenic 35.0%
GATGAAATACTCATGACATC+TGG + chr7_2:2705937-2705956 Msa1044350:intron 35.0%
! GTATTTTACCCACGTAGAAT+TGG - chr7_2:2706027-2706046 None:intergenic 35.0%
!!! TCTTTTGAGTTAGTGCTCTT+GGG - chr7_2:2706074-2706093 None:intergenic 35.0%
TTGTCAATTGCAGAAAACAG+CGG + chr7_2:2706275-2706294 Msa1044350:intron 35.0%
TAGACGCTAAAATAGTGTAG+TGG - chr7_2:2706388-2706407 None:intergenic 35.0%
AGAGCAGTAGATTCAAATAG+AGG - chr7_2:2706447-2706466 None:intergenic 35.0%
TTGAATCTACTGCTCTTGAT+CGG + chr7_2:2706451-2706470 Msa1044350:intron 35.0%
! GGTCTAAAGCTTTATGTACA+AGG - chr7_2:2706515-2706534 None:intergenic 35.0%
TTAGACCTCTGTTGTAAAAG+AGG + chr7_2:2706528-2706547 Msa1044350:intron 35.0%
TTCATGACAAAACCAAGTAC+TGG + chr7_2:2706733-2706752 Msa1044350:CDS 35.0%
GTTGTCAGAAGAAGATCTAG+AGG - chr7_2:2704016-2704035 None:intergenic 40.0%
TCTGTGGCTATGAATTTCAG+AGG + chr7_2:2704136-2704155 Msa1044350:five_prime_UTR 40.0%
GTTTGAAAACATGAGTGTGG+TGG - chr7_2:2704194-2704213 None:intergenic 40.0%
TCAGTACATGCCACATCATT+CGG + chr7_2:2704320-2704339 Msa1044350:five_prime_UTR 40.0%
AATCCAACGAATTCGGAAGA+AGG - chr7_2:2704433-2704452 None:intergenic 40.0%
TTCTTCCGAATTCGTTGGAT+TGG + chr7_2:2704432-2704451 Msa1044350:five_prime_UTR 40.0%
AGACTCCAATCCAACGAATT+CGG - chr7_2:2704440-2704459 None:intergenic 40.0%
! TTAACAAGCAATGGGTTCCA+AGG + chr7_2:2704579-2704598 Msa1044350:exon 40.0%
! CAAGAAGTGTATTCTAGAGG+CGG - chr7_2:2704674-2704693 None:intergenic 40.0%
TACACTTCTTGCTTCAGCAA+CGG + chr7_2:2704683-2704702 Msa1044350:CDS 40.0%
CAAAGACCGTTCTTAAAGCT+CGG + chr7_2:2704706-2704725 Msa1044350:CDS 40.0%
TCTTACACGCTAAATTACGC+AGG + chr7_2:2704757-2704776 Msa1044350:CDS 40.0%
ACAACATGCATAAGAGCCTT+AGG - chr7_2:2704947-2704966 None:intergenic 40.0%
AAGGCTCTTATGCATGTTGT+TGG + chr7_2:2704947-2704966 Msa1044350:CDS 40.0%
GCATGTTGTTGGAACCAAAA+TGG + chr7_2:2704958-2704977 Msa1044350:CDS 40.0%
GTTTACTGAGTGATTGAAGC+CGG + chr7_2:2705158-2705177 Msa1044350:intron 40.0%
GTGAAATGCAACCTATATGC+CGG - chr7_2:2705180-2705199 None:intergenic 40.0%
CATATAGGTTGCATTTCACG+TGG + chr7_2:2705181-2705200 Msa1044350:intron 40.0%
ATTGCACGCTGTTTGTGTTA+TGG + chr7_2:2705266-2705285 Msa1044350:CDS 40.0%
TTGCACGCTGTTTGTGTTAT+GGG + chr7_2:2705267-2705286 Msa1044350:CDS 40.0%
GTTATGGGTCCTTTGTGTAA+TGG + chr7_2:2705282-2705301 Msa1044350:CDS 40.0%
AAGAGTGGTCCATTACACAA+AGG - chr7_2:2705294-2705313 None:intergenic 40.0%
TATTTGTGTTACGGAACCTG+TGG - chr7_2:2705317-2705336 None:intergenic 40.0%
TCCAGTGAGTATGCAATGTT+GGG - chr7_2:2705375-2705394 None:intergenic 40.0%
ATGAACCAGTTGAGGATGAA+TGG - chr7_2:2705416-2705435 None:intergenic 40.0%
!! TCAGATTTGAACGAGTTGGT+AGG + chr7_2:2705478-2705497 Msa1044350:intron 40.0%
GTCGACAAGTTCTATGTTGA+AGG - chr7_2:2705578-2705597 None:intergenic 40.0%
GTGGATAGTTTAATCTGGAC+AGG - chr7_2:2705661-2705680 None:intergenic 40.0%
CTGAGGTGGATAGTTTAATC+TGG - chr7_2:2705666-2705685 None:intergenic 40.0%
!!! CCCACTTTTTCCTAATACTG+AGG - chr7_2:2705683-2705702 None:intergenic 40.0%
TATGCTGGACAACATGAGAA+AGG + chr7_2:2705713-2705732 Msa1044350:intron 40.0%
GTATTCTGCTATGCTATGCT+TGG + chr7_2:2705965-2705984 Msa1044350:intron 40.0%
!! TGCTATGCTTGGTCTTAGTT+AGG + chr7_2:2705976-2705995 Msa1044350:intron 40.0%
TTGCAATCCCAATTCTACGT+GGG + chr7_2:2706016-2706035 Msa1044350:intron 40.0%
!!! GTCTTTTGAGTTAGTGCTCT+TGG - chr7_2:2706075-2706094 None:intergenic 40.0%
TGTTAATTGCGGAAAACAGC+AGG + chr7_2:2706136-2706155 Msa1044350:intron 40.0%
TGTCAATTGCAGAAAACAGC+GGG + chr7_2:2706276-2706295 Msa1044350:intron 40.0%
CAAGGGCTATGCTAAAATAG+AGG - chr7_2:2706497-2706516 None:intergenic 40.0%
ACCTCTGTTGTAAAAGAGGA+AGG + chr7_2:2706532-2706551 Msa1044350:intron 40.0%
! ACCTTCCTCTTTTACAACAG+AGG - chr7_2:2706536-2706555 None:intergenic 40.0%
!! GGAAGGTGTTGATGAATATG+TGG + chr7_2:2706549-2706568 Msa1044350:intron 40.0%
GTGGCATGAATATTAGTGTG+TGG + chr7_2:2706568-2706587 Msa1044350:intron 40.0%
AAGATTCACCACAACCACAT+TGG - chr7_2:2706714-2706733 None:intergenic 40.0%
TCAACCATTTCCTTGCTTAG+AGG - chr7_2:2706761-2706780 None:intergenic 40.0%
TGGTATTTGCATGTTCACCA+TGG - chr7_2:2706840-2706859 None:intergenic 40.0%
! TTATTTTCCTCTGTACTCGC+AGG - chr7_2:2706885-2706904 None:intergenic 40.0%
TTGGCATGCTGATACTGAAA+TGG + chr7_2:2706912-2706931 Msa1044350:three_prime_UTR 40.0%
GACTTGTCGTAGGCTTATAG+CGG - chr7_2:2703963-2703982 None:intergenic 45.0%
!! AGGTGTTGTTGACTTGTCGT+AGG - chr7_2:2703973-2703992 None:intergenic 45.0%
TTTCCAACTCATGCGCCTAA+TGG + chr7_2:2704089-2704108 Msa1044350:five_prime_UTR 45.0%
CCTCGCGAAATCACGAAATA+CGG - chr7_2:2704221-2704240 None:intergenic 45.0%
! CGGTGCTAAATATAGCGAAC+AGG - chr7_2:2704247-2704266 None:intergenic 45.0%
CAGTACATGCCACATCATTC+GGG + chr7_2:2704321-2704340 Msa1044350:five_prime_UTR 45.0%
AATTGTTGTGCGTATGGCAG+TGG - chr7_2:2704406-2704425 None:intergenic 45.0%
GGTGGAAATTGTTGTGCGTA+TGG - chr7_2:2704412-2704431 None:intergenic 45.0%
! GAATTCGTTGGATTGGAGTC+TGG + chr7_2:2704439-2704458 Msa1044350:five_prime_UTR 45.0%
CTGAGATTGAACGGTGTTTG+GGG - chr7_2:2704548-2704567 None:intergenic 45.0%
GCTGAGATTGAACGGTGTTT+GGG - chr7_2:2704549-2704568 None:intergenic 45.0%
TGCTGAGATTGAACGGTGTT+TGG - chr7_2:2704550-2704569 None:intergenic 45.0%
!! TTGACGCCGAGCTTTAAGAA+CGG - chr7_2:2704715-2704734 None:intergenic 45.0%
! AAACATTGATTGGGGGAGGA+GGG + chr7_2:2705104-2705123 Msa1044350:intron 45.0%
AGTGATTGAAGCCGGCATAT+AGG + chr7_2:2705166-2705185 Msa1044350:intron 45.0%
GTCCAGTGAGTATGCAATGT+TGG - chr7_2:2705376-2705395 None:intergenic 45.0%
ACATTGCATACTCACTGGAC+AGG + chr7_2:2705376-2705395 Msa1044350:intron 45.0%
! ATGTGCCATTCATCCTCAAC+TGG + chr7_2:2705408-2705427 Msa1044350:intron 45.0%
ACTCGTTCAAATCTGACCAC+AGG - chr7_2:2705474-2705493 None:intergenic 45.0%
GTGGTCAGATTTGAACGAGT+TGG + chr7_2:2705474-2705493 Msa1044350:intron 45.0%
!! CTAGCTTGGAGGATTTGTTG+CGG - chr7_2:2705507-2705526 None:intergenic 45.0%
CAAAGGTTCGTCATCTAGCT+TGG - chr7_2:2705521-2705540 None:intergenic 45.0%
CAAGCTAGATGACGAACCTT+TGG + chr7_2:2705519-2705538 Msa1044350:intron 45.0%
GAAGGAGATGGGATAGCATA+TGG - chr7_2:2705560-2705579 None:intergenic 45.0%
ATCTAATGATCCCGTGTGAG+AGG - chr7_2:2705771-2705790 None:intergenic 45.0%
TGATGTAGGATCCATGGTTG+CGG - chr7_2:2705860-2705879 None:intergenic 45.0%
GTTGCAATCCCAATTCTACG+TGG + chr7_2:2706015-2706034 Msa1044350:intron 45.0%
! GTGTCTTTTGAGCTGCTCTT+GGG - chr7_2:2706217-2706236 None:intergenic 45.0%
! AGTGTCTTTTGAGCTGCTCT+TGG - chr7_2:2706218-2706237 None:intergenic 45.0%
!! AAATAGTGTAGTGGACTCCG+TGG - chr7_2:2706379-2706398 None:intergenic 45.0%
! AATTTTGCCTGCGAGTACAG+AGG + chr7_2:2706875-2706894 Msa1044350:three_prime_UTR 45.0%
!! CTCTGAAGCTGATACTGACA+AGG + chr7_2:2706952-2706971 Msa1044350:three_prime_UTR 45.0%
GTTCCATTAGGCGCATGAGT+TGG - chr7_2:2704095-2704114 None:intergenic 50.0%
CCGTATTTCGTGATTTCGCG+AGG + chr7_2:2704218-2704237 Msa1044350:five_prime_UTR 50.0%
CGAAAACGTCCCGAATGATG+TGG - chr7_2:2704333-2704352 None:intergenic 50.0%
CCAACGAATTCGGAAGAAGG+TGG - chr7_2:2704430-2704449 None:intergenic 50.0%
CCACCTTCTTCCGAATTCGT+TGG + chr7_2:2704427-2704446 Msa1044350:five_prime_UTR 50.0%
!! GTTCGTCATTTTGGATCGCG+TGG - chr7_2:2704468-2704487 None:intergenic 50.0%
GGCAGCAGAGAAGATAAGCA+CGG + chr7_2:2704612-2704631 Msa1044350:CDS 50.0%
AGCAGAGAAGATAAGCACGG+CGG + chr7_2:2704615-2704634 Msa1044350:CDS 50.0%
! GAAGTGTATTCTAGAGGCGG+CGG - chr7_2:2704671-2704690 None:intergenic 50.0%
GTCAAAGCTCGCGGATGTAA+TGG + chr7_2:2704730-2704749 Msa1044350:CDS 50.0%
! ACGCAAACATTGATTGGGGG+AGG + chr7_2:2705100-2705119 Msa1044350:intron 50.0%
! CAAACATTGATTGGGGGAGG+AGG + chr7_2:2705103-2705122 Msa1044350:intron 50.0%
!! GTAATGGACCACTCTTCCAC+AGG + chr7_2:2705298-2705317 Msa1044350:CDS 50.0%
TTACGGAACCTGTGGAAGAG+TGG - chr7_2:2705309-2705328 None:intergenic 50.0%
GCCCAACATTGCATACTCAC+TGG + chr7_2:2705371-2705390 Msa1044350:intron 50.0%
!! AGGGCATTGAACATGACCTG+TGG + chr7_2:2705455-2705474 Msa1044350:intron 50.0%
! AGGTTCGTCATCTAGCTTGG+AGG - chr7_2:2705518-2705537 None:intergenic 50.0%
GCTAGATGACGAACCTTTGG+TGG + chr7_2:2705522-2705541 Msa1044350:intron 50.0%
GTTTAATCTGGACAGGCGAG+AGG - chr7_2:2705654-2705673 None:intergenic 50.0%
GTGGGTCCGACAAAATATGC+TGG + chr7_2:2705698-2705717 Msa1044350:intron 50.0%
TAATGATCCCGTGTGAGAGG+AGG - chr7_2:2705768-2705787 None:intergenic 50.0%
ATGGTTGCGGGTGGAAGATT+CGG - chr7_2:2705847-2705866 None:intergenic 50.0%
GATGTAGGATCCATGGTTGC+GGG - chr7_2:2705859-2705878 None:intergenic 50.0%
TGAGGGTGATGTAGGATCCA+TGG - chr7_2:2705866-2705885 None:intergenic 50.0%
!! CCTAATTGGTGATGGCGCTA+GGG - chr7_2:2705889-2705908 None:intergenic 50.0%
!! TCCTAATTGGTGATGGCGCT+AGG - chr7_2:2705890-2705909 None:intergenic 50.0%
GAACTCAGATCTGCCAGAAG+AGG - chr7_2:2706665-2706684 None:intergenic 50.0%
!! CCACATTGGCCTTTGGAGTT+TGG - chr7_2:2706700-2706719 None:intergenic 50.0%
CCAAACTCCAAAGGCCAATG+TGG + chr7_2:2706697-2706716 Msa1044350:CDS 50.0%
TCCAAAGGCCAATGTGGTTG+TGG + chr7_2:2706703-2706722 Msa1044350:CDS 50.0%
!! ACCACAACCACATTGGCCTT+TGG - chr7_2:2706707-2706726 None:intergenic 50.0%
TGCTTAGAGGCTCCAGTACT+TGG - chr7_2:2706748-2706767 None:intergenic 50.0%
! AGTACTGGAGCCTCTAAGCA+AGG + chr7_2:2706748-2706767 Msa1044350:CDS 50.0%
GGAGCCTCTAAGCAAGGAAA+TGG + chr7_2:2706754-2706773 Msa1044350:CDS 50.0%
! AGCAATGGGTTCCAAGGCGA+TGG + chr7_2:2704585-2704604 Msa1044350:exon 55.0%
!! GGCGGCATCGGTTAACTCAA+CGG - chr7_2:2704653-2704672 None:intergenic 55.0%
! TATTCTAGAGGCGGCGGCAT+CGG - chr7_2:2704665-2704684 None:intergenic 55.0%
!! GTGTGCGAGAGTACCACCAA+AGG - chr7_2:2705538-2705557 None:intergenic 55.0%
ATCTGGACAGGCGAGAGGAA+TGG - chr7_2:2705649-2705668 None:intergenic 55.0%
ACATCCCACCTCCTCTCACA+CGG + chr7_2:2705757-2705776 Msa1044350:intron 55.0%
! TGCGGGTGGAAGATTCGGTT+TGG - chr7_2:2705842-2705861 None:intergenic 55.0%
GAATCTTCCACCCGCAACCA+TGG + chr7_2:2705846-2705865 Msa1044350:intron 55.0%
CCCTAGCGCCATCACCAATT+AGG + chr7_2:2705886-2705905 Msa1044350:intron 55.0%
GGGTTCCAAGGCGATGGCTA+TGG + chr7_2:2704591-2704610 Msa1044350:CDS 60.0%
TGCTGCCATAGCCATCGCCT+TGG - chr7_2:2704599-2704618 None:intergenic 60.0%
AGATAAGCACGGCGGTGCGA+CGG + chr7_2:2704623-2704642 Msa1044350:CDS 60.0%
AAGCTCGGCGTCAAAGCTCG+CGG + chr7_2:2704721-2704740 Msa1044350:CDS 60.0%
TCACGTGGGTGACTGTGCTG+AGG + chr7_2:2705196-2705215 Msa1044350:CDS 60.0%
CATCCCACCTCCTCTCACAC+GGG + chr7_2:2705758-2705777 Msa1044350:intron 60.0%
GATCCCGTGTGAGAGGAGGT+GGG - chr7_2:2705764-2705783 None:intergenic 60.0%
TGATCCCGTGTGAGAGGAGG+TGG - chr7_2:2705765-2705784 None:intergenic 60.0%
GTAGGATCCATGGTTGCGGG+TGG - chr7_2:2705856-2705875 None:intergenic 60.0%
CGCTAGGGTGAGGGTGATGT+AGG - chr7_2:2705874-2705893 None:intergenic 60.0%
!! TGGTGATGGCGCTAGGGTGA+GGG - chr7_2:2705883-2705902 None:intergenic 60.0%
!! TTGGTGATGGCGCTAGGGTG+AGG - chr7_2:2705884-2705903 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 2703960 2707086 2703960 ID=Msa1044350;Name=Msa1044350
chr7_2 mRNA 2703960 2707086 2703960 ID=Msa1044350-mRNA-1;Parent=Msa1044350;Name=Msa1044350-mRNA-1;_AED=0.05;_eAED=0.05;_QI=629|0.33|0.75|0.75|1|1|4|306|181
chr7_2 exon 2703960 2704778 2703960 ID=Msa1044350-mRNA-1:exon:687;Parent=Msa1044350-mRNA-1
chr7_2 exon 2704885 2705002 2704885 ID=Msa1044350-mRNA-1:exon:688;Parent=Msa1044350-mRNA-1
chr7_2 exon 2705188 2705319 2705188 ID=Msa1044350-mRNA-1:exon:689;Parent=Msa1044350-mRNA-1
chr7_2 exon 2706675 2707086 2706675 ID=Msa1044350-mRNA-1:exon:690;Parent=Msa1044350-mRNA-1
chr7_2 five_prime_UTR 2703960 2704588 2703960 ID=Msa1044350-mRNA-1:five_prime_utr;Parent=Msa1044350-mRNA-1
chr7_2 CDS 2704589 2704778 2704589 ID=Msa1044350-mRNA-1:cds;Parent=Msa1044350-mRNA-1
chr7_2 CDS 2704885 2705002 2704885 ID=Msa1044350-mRNA-1:cds;Parent=Msa1044350-mRNA-1
chr7_2 CDS 2705188 2705319 2705188 ID=Msa1044350-mRNA-1:cds;Parent=Msa1044350-mRNA-1
chr7_2 CDS 2706675 2706780 2706675 ID=Msa1044350-mRNA-1:cds;Parent=Msa1044350-mRNA-1
chr7_2 three_prime_UTR 2706781 2707086 2706781 ID=Msa1044350-mRNA-1:three_prime_utr;Parent=Msa1044350-mRNA-1
Gene Sequence

>Msa1044350

ATGGGTTCCAAGGCGATGGCTATGGCAGCAGAGAAGATAAGCACGGCGGTGCGACGGCAAGCCGTTGAGTTAACCGATGCCGCCGCCTCTAGAATACACTTCTTGCTTCAGCAACGGCAAAGACCGTTCTTAAAGCTCGGCGTCAAAGCTCGCGGATGTAATGGTTTATCTTACACGCTAAATTACGCAGATAAAAAAGAGAAATTTGATGAATTGGTTGAAGATAAAGGTGTTAAGATATTGATTGATCCTAAGGCTCTTATGCATGTTGTTGGAACCAAAATGGATTTCGTTGATGATAAACTAAGGTTGCATTTCACGTGGGTGACTGTGCTGAGGTCGTTTCTGTATGTCGTTGTTGTTCGTGCTGTTATTGTTCACAGCAGATTGCACGCTGTTTGTGTTATGGGTCCTTTGTGTAATGGACCACTCTTCCACAGATCTGAGTTCGTTTTCATTAATCCAAACTCCAAAGGCCAATGTGGTTGTGGTGAATCTTTCATGACAAAACCAAGTACTGGAGCCTCTAAGCAAGGAAATGGTTGA
Protein sequence

>Msa1044350

MGSKAMAMAAEKISTAVRRQAVELTDAAASRIHFLLQQRQRPFLKLGVKARGCNGLSYTLNYADKKEKFDELVEDKGVKILIDPKALMHVVGTKMDFVDDKLRLHFTWVTVLRSFLYVVVVRAVIVHSRLHAVCVMGPLCNGPLFHRSEFVFINPNSKGQCGCGESFMTKPSTGASKQGNG*