AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0163560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0163560 MtrunA17_Chr1g0178671 98.540 137 2 0 1 137 1 137 5.94e-100 282
Msa0163560 MtrunA17_Chr8g0383271 36.634 101 61 2 22 119 70 170 4.65e-18 75.9
Msa0163560 MtrunA17_Chr7g0266291 35.714 84 53 1 44 126 76 159 4.54e-14 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0163560 AT2G16710.1 75.188 133 32 1 2 134 3 134 4.43e-72 212
Msa0163560 AT2G16710.3 72.464 138 31 2 3 134 4 140 1.49e-69 206
Msa0163560 AT2G36260.1 69.725 109 32 1 17 125 1 108 1.03e-55 170
Msa0163560 AT2G16710.2 73.529 102 27 0 2 103 3 104 1.33e-51 159
Msa0163560 AT1G10500.1 32.407 108 70 2 19 123 69 176 8.10e-18 75.9
Msa0163560 AT5G03905.1 31.481 108 72 2 21 126 50 157 4.85e-13 62.8

Find 86 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGGTATGTTCGTCATTT+TGG 0.177213 1_4:-41829114 None:intergenic
ATCATCAACGAAATCCATTT+TGG 0.208042 1_4:-41829612 None:intergenic
CCACATTGGCCTTTGGAGTT+TGG 0.248774 1_4:-41831337 None:intergenic
TCTCTATTTCTTCTATTTCA+TGG 0.257785 1_4:+41828677 Msa0163560:five_prime_UTR
AAAAGAGAAATTTGATGAAT+TGG 0.258711 1_4:+41829529 Msa0163560:CDS
TCAACCATTTCCTTGCTTAG+AGG 0.300532 1_4:-41831398 None:intergenic
GCATGTTGTTGGAACCAAAA+TGG 0.312759 1_4:+41829598 Msa0163560:CDS
TGCTTAGAGGCTCCAGTACT+TGG 0.316007 1_4:-41831385 None:intergenic
TTTCCAACTCATGCGCCTAA+TGG 0.325435 1_4:+41828729 Msa0163560:five_prime_UTR
TGCTGAGATTGAACGGTGTT+TGG 0.336566 1_4:-41829187 None:intergenic
GTGGATACTGTATAATTTGT+AGG 0.368896 1_4:+41831678 Msa0163560:three_prime_UTR
TCAGTACATGCCACATCATT+CGG 0.376103 1_4:+41828960 Msa0163560:five_prime_UTR
TTTAGTCATTTGAACATGTA+AGG 0.381086 1_4:-41829142 None:intergenic
TATTTCTTCTATTTCATGGT+TGG 0.382503 1_4:+41828681 Msa0163560:five_prime_UTR
TTCTTCCGAATTCGTTGGAT+TGG 0.383312 1_4:+41829072 Msa0163560:five_prime_UTR
GATGAATTGGTTGAAGATAA+AGG 0.389292 1_4:+41829542 Msa0163560:CDS
ACCACAACCACATTGGCCTT+TGG 0.397048 1_4:-41831344 None:intergenic
AGACTCCAATCCAACGAATT+CGG 0.422958 1_4:-41829077 None:intergenic
AATGACTAAATTAACCTCAT+AGG 0.422962 1_4:+41829155 Msa0163560:five_prime_UTR
AAAATAGATGATGGTATTCA+AGG 0.433143 1_4:-41828754 None:intergenic
GCTGAGATTGAACGGTGTTT+GGG 0.436035 1_4:-41829186 None:intergenic
ATGGTATTCAAGGTTCCATT+AGG 0.437536 1_4:-41828744 None:intergenic
GAATTCGTTGGATTGGAGTC+TGG 0.444757 1_4:+41829079 Msa0163560:five_prime_UTR
TTTCGTAATTAACAAGCAAT+GGG 0.449583 1_4:+41829211 Msa0163560:exon
TTGGCATGCTGATACTGAAA+TGG 0.458736 1_4:+41831552 Msa0163560:three_prime_UTR
GGAGCCTCTAAGCAAGGAAA+TGG 0.461818 1_4:+41831394 Msa0163560:CDS
AAGGCTCTTATGCATGTTGT+TGG 0.463150 1_4:+41829587 Msa0163560:CDS
ATTTCGTAATTAACAAGCAA+TGG 0.463321 1_4:+41829210 Msa0163560:exon
CAGTACATGCCACATCATTC+GGG 0.468012 1_4:+41828961 Msa0163560:five_prime_UTR
CCACCTTCTTCCGAATTCGT+TGG 0.476252 1_4:+41829067 Msa0163560:five_prime_UTR
TTTGGGCATTTATACCTATG+AGG 0.479572 1_4:-41829169 None:intergenic
CGGTGCTAAATATAGCGAAC+AGG 0.479621 1_4:-41828884 None:intergenic
ACAACATGCATAAGAGCCTT+AGG 0.481683 1_4:-41829584 None:intergenic
AGGAAATTACATAGAGAAGA+AGG 0.487338 1_4:-41828633 None:intergenic
TGAACATGTAAGGACATGTA+AGG 0.491010 1_4:-41829132 None:intergenic
CGCTGCCATAGCCATCGCCT+TGG 0.499390 1_4:-41829236 None:intergenic
ATTTCGTTGATGATAAACTA+AGG 0.504621 1_4:+41829621 Msa0163560:CDS
GTCAAAGCTCGCGGATGTAA+TGG 0.505977 1_4:+41829370 Msa0163560:CDS
TTCATGACAAAACCAAGTAC+TGG 0.509644 1_4:+41831373 Msa0163560:CDS
GGGTTCCAAGGCGATGGCTA+TGG 0.513236 1_4:+41829231 Msa0163560:CDS
TCCAAAGGCCAATGTGGTTG+TGG 0.524509 1_4:+41831343 Msa0163560:CDS
CCTCGCGAAATCACGAAATA+CGG 0.525892 1_4:-41828858 None:intergenic
AATCCAACGAATTCGGAAGA+AGG 0.529950 1_4:-41829070 None:intergenic
TTCATTAATCCAAACTCCAA+AGG 0.536589 1_4:+41831328 Msa0163560:CDS
GTTGTCAGAAGAAGATCTAG+AGG 0.539767 1_4:-41828653 None:intergenic
ACGAAATTGCTGAGATTGAA+CGG 0.542457 1_4:-41829194 None:intergenic
CAAGGCGATGGCTATGGCAG+CGG 0.545268 1_4:+41829237 Msa0163560:CDS
AGGTGTTGTTGACTTGTCGT+AGG 0.551502 1_4:-41828610 None:intergenic
TATTCTAGAGGCGGCGGCAT+CGG 0.552495 1_4:-41829302 None:intergenic
GTTCCATTAGGCGCATGAGT+TGG 0.558660 1_4:-41828732 None:intergenic
CAAAGACCGTTCTTAAAGCT+CGG 0.558956 1_4:+41829346 Msa0163560:CDS
TTAACAAGCAATGGGTTCCA+AGG 0.561441 1_4:+41829219 Msa0163560:exon
TACACTTCTTGCTTCAGCAA+CGG 0.562185 1_4:+41829323 Msa0163560:CDS
GTTGAAATTATCATTCAACT+AGG 0.573251 1_4:+41831416 Msa0163560:exon
AGCAATGGGTTCCAAGGCGA+TGG 0.573401 1_4:+41829225 Msa0163560:exon
AGATAAGCACGGCGGTGCGA+CGG 0.576266 1_4:+41829263 Msa0163560:CDS
AATTGTTGTGCGTATGGCAG+TGG 0.579932 1_4:-41829043 None:intergenic
GGTGGAAATTGTTGTGCGTA+TGG 0.580291 1_4:-41829049 None:intergenic
GGCGGCATCGGTTAACTCAA+CGG 0.586444 1_4:-41829290 None:intergenic
TCTGTGGCTATGAATTTCAG+AGG 0.588281 1_4:+41828776 Msa0163560:five_prime_UTR
CTCTGAAGCTGATACTGACA+AGG 0.590200 1_4:+41831592 Msa0163560:three_prime_UTR
TAGCCACAGAAAATAGATGA+TGG 0.591925 1_4:-41828763 None:intergenic
CCAACGAATTCGGAAGAAGG+TGG 0.596750 1_4:-41829067 None:intergenic
AAATTACATAGAGAAGAAGG+AGG 0.601528 1_4:-41828630 None:intergenic
GAAGTGTATTCTAGAGGCGG+CGG 0.606928 1_4:-41829308 None:intergenic
TTTAATTTAATGAGAAAGAG+CGG 0.607923 1_4:-41828904 None:intergenic
TTGTCGTAGGCTTATAGCGG+TGG 0.610408 1_4:-41828597 None:intergenic
AAATTACAGAAATTCAATTG+TGG 0.623213 1_4:-41831497 None:intergenic
AGTACTGGAGCCTCTAAGCA+AGG 0.624921 1_4:+41831388 Msa0163560:CDS
AAGATTCACCACAACCACAT+TGG 0.628045 1_4:-41831351 None:intergenic
CCAAACTCCAAAGGCCAATG+TGG 0.629156 1_4:+41831337 Msa0163560:CDS
GACTTGTCGTAGGCTTATAG+CGG 0.631925 1_4:-41828600 None:intergenic
CCGTATTTCGTGATTTCGCG+AGG 0.633478 1_4:+41828858 Msa0163560:five_prime_UTR
GAACATGTAAGGACATGTAA+GGG 0.635484 1_4:-41829131 None:intergenic
CGAAAACGTCCCGAATGATG+TGG 0.636787 1_4:-41828970 None:intergenic
CAAGAAGTGTATTCTAGAGG+CGG 0.641303 1_4:-41829311 None:intergenic
TGGTATTTGCATGTTCACCA+TGG 0.645858 1_4:-41831477 None:intergenic
AAGCAAGAAGTGTATTCTAG+AGG 0.646366 1_4:-41829314 None:intergenic
TTGACGCCGAGCTTTAAGAA+CGG 0.652738 1_4:-41829352 None:intergenic
AAGCTCGGCGTCAAAGCTCG+CGG 0.657327 1_4:+41829361 Msa0163560:CDS
TCTTACACGCTAAATTACGC+AGG 0.659336 1_4:+41829397 Msa0163560:CDS
GGCAGCGGAGAAGATAAGCA+CGG 0.676966 1_4:+41829252 Msa0163560:CDS
GTTTGAAAACATGAGTGTGG+TGG 0.682217 1_4:-41828831 None:intergenic
TCAGTTTGAAAACATGAGTG+TGG 0.728519 1_4:-41828834 None:intergenic
TTATTGAAGAGAGAATCGAA+AGG 0.762981 1_4:-41828804 None:intergenic
AGCGGAGAAGATAAGCACGG+CGG 0.763606 1_4:+41829255 Msa0163560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATTAAAATTAAAAA+AGG + chr1_4:41828921-41828940 Msa0163560:five_prime_UTR 0.0%
!! ATTAAACGAAAATTTAAAGA+GGG - chr1_4:41829436-41829455 None:intergenic 15.0%
!! AATTAAACGAAAATTTAAAG+AGG - chr1_4:41829437-41829456 None:intergenic 15.0%
!!! TTTAATTTAATGAGAAAGAG+CGG - chr1_4:41828907-41828926 None:intergenic 20.0%
!!! GTATTGATTTTAAGATTTGT+TGG + chr1_4:41829488-41829507 Msa0163560:intron 20.0%
!! AAAAGAGAAATTTGATGAAT+TGG + chr1_4:41829529-41829548 Msa0163560:CDS 20.0%
!!! TTCTTTGTTCAATTTTGTTT+TGG + chr1_4:41829682-41829701 Msa0163560:intron 20.0%
!!! TTTTTTTTCCCCAATAAATA+AGG - chr1_4:41829721-41829740 None:intergenic 20.0%
!! TAATTATATGATGAGGTTTA+TGG - chr1_4:41830242-41830261 None:intergenic 20.0%
!!! TTGCTTATGTTTTTTCTTTT+CGG + chr1_4:41831254-41831273 Msa0163560:intron 20.0%
!! AAATTACAGAAATTCAATTG+TGG - chr1_4:41831500-41831519 None:intergenic 20.0%
!! ATTCATGTAAAAAACTATTG+TGG + chr1_4:41831659-41831678 Msa0163560:three_prime_UTR 20.0%
!! TCTCTATTTCTTCTATTTCA+TGG + chr1_4:41828677-41828696 Msa0163560:five_prime_UTR 25.0%
! TATTTCTTCTATTTCATGGT+TGG + chr1_4:41828681-41828700 Msa0163560:five_prime_UTR 25.0%
!! AAAATAGATGATGGTATTCA+AGG - chr1_4:41828757-41828776 None:intergenic 25.0%
! TTAAAAAAGGAAAATGCAAG+TGG + chr1_4:41828934-41828953 Msa0163560:five_prime_UTR 25.0%
! TAAAAAAGGAAAATGCAAGT+GGG + chr1_4:41828935-41828954 Msa0163560:five_prime_UTR 25.0%
! TTTAGTCATTTGAACATGTA+AGG - chr1_4:41829145-41829164 None:intergenic 25.0%
! AATGACTAAATTAACCTCAT+AGG + chr1_4:41829155-41829174 Msa0163560:five_prime_UTR 25.0%
! ATTTCGTAATTAACAAGCAA+TGG + chr1_4:41829210-41829229 Msa0163560:exon 25.0%
! TTTCGTAATTAACAAGCAAT+GGG + chr1_4:41829211-41829230 Msa0163560:exon 25.0%
!!! TTGATTTTAAGATTTGTTGG+TGG + chr1_4:41829491-41829510 Msa0163560:intron 25.0%
!!! TAAGATTTTGATTGATCCTA+AGG + chr1_4:41829568-41829587 Msa0163560:CDS 25.0%
!!! ATTTCGTTGATGATAAACTA+AGG + chr1_4:41829621-41829640 Msa0163560:CDS 25.0%
!! TCTTGTTCAACCTTATTTAT+TGG + chr1_4:41829708-41829727 Msa0163560:intron 25.0%
!! CTTGTTCAACCTTATTTATT+GGG + chr1_4:41829709-41829728 Msa0163560:intron 25.0%
!! TTGTTCAACCTTATTTATTG+GGG + chr1_4:41829710-41829729 Msa0163560:intron 25.0%
! AAAAAAACGCAAACATTGAT+TGG + chr1_4:41829734-41829753 Msa0163560:intron 25.0%
!! AAAAAACGCAAACATTGATT+GGG + chr1_4:41829735-41829754 Msa0163560:intron 25.0%
! AGTATGTCATAAATCTCATT+TGG + chr1_4:41830439-41830458 Msa0163560:intron 25.0%
! AAGTAATGCATATTCCTAAT+TGG - chr1_4:41830543-41830562 None:intergenic 25.0%
! TAGGAATATGCATTACTTAA+AGG + chr1_4:41830545-41830564 Msa0163560:intron 25.0%
! AGGAATATGCATTACTTAAA+GGG + chr1_4:41830546-41830565 Msa0163560:intron 25.0%
! TACATCAATGTTGTTAATTG+CGG + chr1_4:41830765-41830784 Msa0163560:intron 25.0%
! GTTGAAATTATCATTCAACT+AGG + chr1_4:41831416-41831435 Msa0163560:exon 25.0%
!! TCTTTCTTTTATGATTTCCA+TGG + chr1_4:41831460-41831479 Msa0163560:three_prime_UTR 25.0%
! AGAGGAAAATAAAAAAGCTT+TGG + chr1_4:41831533-41831552 Msa0163560:three_prime_UTR 25.0%
AAATTACATAGAGAAGAAGG+AGG - chr1_4:41828633-41828652 None:intergenic 30.0%
AGGAAATTACATAGAGAAGA+AGG - chr1_4:41828636-41828655 None:intergenic 30.0%
! TGAGTTGGAAAGAAAAACTA+TGG - chr1_4:41828720-41828739 None:intergenic 30.0%
! ATACCATCATCTATTTTCTG+TGG + chr1_4:41828760-41828779 Msa0163560:five_prime_UTR 30.0%
TTATTGAAGAGAGAATCGAA+AGG - chr1_4:41828807-41828826 None:intergenic 30.0%
GATGAATTGGTTGAAGATAA+AGG + chr1_4:41829542-41829561 Msa0163560:CDS 30.0%
! ATCATCAACGAAATCCATTT+TGG - chr1_4:41829615-41829634 None:intergenic 30.0%
! AAAAACGCAAACATTGATTG+GGG + chr1_4:41829736-41829755 Msa0163560:intron 30.0%
TCAGTAAACCTTACTAGTTA+AGG - chr1_4:41829787-41829806 None:intergenic 30.0%
ATTATGTAATGAACCAGTTG+AGG - chr1_4:41830064-41830083 None:intergenic 30.0%
! TTACATAATTTGCATGCTAC+AGG + chr1_4:41830075-41830094 Msa0163560:intron 30.0%
! TACATAATTTGCATGCTACA+GGG + chr1_4:41830076-41830095 Msa0163560:intron 30.0%
CCTCATCATATAATTATACC+CGG + chr1_4:41830246-41830265 Msa0163560:intron 30.0%
! TATTTTACCCACGTAGAATT+GGG - chr1_4:41830666-41830685 None:intergenic 30.0%
ATTAGTTACTGCTTGATCTT+TGG + chr1_4:41830885-41830904 Msa0163560:intron 30.0%
AATATGCTACAATGCTATAG+CGG + chr1_4:41830952-41830971 Msa0163560:intron 30.0%
TTGTACTAAATAGCATACCA+CGG + chr1_4:41830999-41831018 Msa0163560:intron 30.0%
! GTCTAAAGCTTTATGTACAA+GGG - chr1_4:41831154-41831173 None:intergenic 30.0%
AAAAAACATAAGCAAGTAGC+AGG - chr1_4:41831249-41831268 None:intergenic 30.0%
! AAATACTATTTCTCCTCTTC+TGG + chr1_4:41831289-41831308 Msa0163560:intron 30.0%
TTCATTAATCCAAACTCCAA+AGG + chr1_4:41831328-41831347 Msa0163560:CDS 30.0%
! GTGGATACTGTATAATTTGT+AGG + chr1_4:41831678-41831697 Msa0163560:three_prime_UTR 30.0%
ATGGTATTCAAGGTTCCATT+AGG - chr1_4:41828747-41828766 None:intergenic 35.0%
TAGCCACAGAAAATAGATGA+TGG - chr1_4:41828766-41828785 None:intergenic 35.0%
TCAGTTTGAAAACATGAGTG+TGG - chr1_4:41828837-41828856 None:intergenic 35.0%
!! TAAGGGTATGTTCGTCATTT+TGG - chr1_4:41829117-41829136 None:intergenic 35.0%
GAACATGTAAGGACATGTAA+GGG - chr1_4:41829134-41829153 None:intergenic 35.0%
TGAACATGTAAGGACATGTA+AGG - chr1_4:41829135-41829154 None:intergenic 35.0%
TTTGGGCATTTATACCTATG+AGG - chr1_4:41829172-41829191 None:intergenic 35.0%
ACGAAATTGCTGAGATTGAA+CGG - chr1_4:41829197-41829216 None:intergenic 35.0%
! AAGCAAGAAGTGTATTCTAG+AGG - chr1_4:41829317-41829336 None:intergenic 35.0%
! AAAACGCAAACATTGATTGG+GGG + chr1_4:41829737-41829756 Msa0163560:intron 35.0%
ACTAGTTAAGGCTTCAAACA+TGG - chr1_4:41829775-41829794 None:intergenic 35.0%
! GTTTGAAGCCTTAACTAGTA+AGG + chr1_4:41829776-41829795 Msa0163560:intron 35.0%
GTTTACTGAGTGATTGAAGA+AGG + chr1_4:41829798-41829817 Msa0163560:intron 35.0%
! AGTGATTGAAGAAGGCATAT+AGG + chr1_4:41829806-41829825 Msa0163560:intron 35.0%
ATATAGGTTGCATTTCACGT+GGG + chr1_4:41829822-41829841 Msa0163560:intron 35.0%
ATTGCACGCTATTTGTGTTA+CGG - chr1_4:41829966-41829985 None:intergenic 35.0%
AGTTCTATGTTGAAGGAGAT+GGG - chr1_4:41830211-41830230 None:intergenic 35.0%
AAGTTCTATGTTGAAGGAGA+TGG - chr1_4:41830212-41830231 None:intergenic 35.0%
CCGGGTATAATTATATGATG+AGG - chr1_4:41830249-41830268 None:intergenic 35.0%
! GTATTTATTCGGTTTGAACC+GGG - chr1_4:41830267-41830286 None:intergenic 35.0%
! GGTATTTATTCGGTTTGAAC+CGG - chr1_4:41830268-41830287 None:intergenic 35.0%
CGAGAGGAATGGTATTTATT+CGG - chr1_4:41830278-41830297 None:intergenic 35.0%
AAACTATCCACCTCAGTATT+AGG + chr1_4:41830310-41830329 Msa0163560:intron 35.0%
!! ACTTTTTCCTAATACTGAGG+TGG - chr1_4:41830320-41830339 None:intergenic 35.0%
ACCTCAGTATTAGGAAAAAG+TGG + chr1_4:41830319-41830338 Msa0163560:intron 35.0%
CCTCAGTATTAGGAAAAAGT+GGG + chr1_4:41830320-41830339 Msa0163560:intron 35.0%
! TGTTGTCCAGCATATTTTGT+CGG - chr1_4:41830347-41830366 None:intergenic 35.0%
TGCATATTCCTAATTGGTGA+TGG - chr1_4:41830537-41830556 None:intergenic 35.0%
GATGAAATACTCATGACATC+TGG + chr1_4:41830577-41830596 Msa0163560:intron 35.0%
! GTATTTTACCCACGTAGAAT+TGG - chr1_4:41830667-41830686 None:intergenic 35.0%
!!! TCTTTTGAGTTAGTGCTCTT+GGG - chr1_4:41830714-41830733 None:intergenic 35.0%
TTGTCAATTGCAGAAAACAG+CGG + chr1_4:41830915-41830934 Msa0163560:intron 35.0%
TAGACGCTAAAATAGTGTAG+TGG - chr1_4:41831028-41831047 None:intergenic 35.0%
AGAGCAGTAGATTCAAATAG+AGG - chr1_4:41831087-41831106 None:intergenic 35.0%
TTGAATCTACTGCTCTTGAT+CGG + chr1_4:41831091-41831110 Msa0163560:intron 35.0%
! GGTCTAAAGCTTTATGTACA+AGG - chr1_4:41831155-41831174 None:intergenic 35.0%
TTAGACCTCTGTTGTAAAAG+AGG + chr1_4:41831168-41831187 Msa0163560:intron 35.0%
TTCATGACAAAACCAAGTAC+TGG + chr1_4:41831373-41831392 Msa0163560:CDS 35.0%
GTTGTCAGAAGAAGATCTAG+AGG - chr1_4:41828656-41828675 None:intergenic 40.0%
TCTGTGGCTATGAATTTCAG+AGG + chr1_4:41828776-41828795 Msa0163560:five_prime_UTR 40.0%
GTTTGAAAACATGAGTGTGG+TGG - chr1_4:41828834-41828853 None:intergenic 40.0%
TCAGTACATGCCACATCATT+CGG + chr1_4:41828960-41828979 Msa0163560:five_prime_UTR 40.0%
AATCCAACGAATTCGGAAGA+AGG - chr1_4:41829073-41829092 None:intergenic 40.0%
TTCTTCCGAATTCGTTGGAT+TGG + chr1_4:41829072-41829091 Msa0163560:five_prime_UTR 40.0%
AGACTCCAATCCAACGAATT+CGG - chr1_4:41829080-41829099 None:intergenic 40.0%
! TTAACAAGCAATGGGTTCCA+AGG + chr1_4:41829219-41829238 Msa0163560:exon 40.0%
! CAAGAAGTGTATTCTAGAGG+CGG - chr1_4:41829314-41829333 None:intergenic 40.0%
TACACTTCTTGCTTCAGCAA+CGG + chr1_4:41829323-41829342 Msa0163560:CDS 40.0%
CAAAGACCGTTCTTAAAGCT+CGG + chr1_4:41829346-41829365 Msa0163560:CDS 40.0%
TCTTACACGCTAAATTACGC+AGG + chr1_4:41829397-41829416 Msa0163560:CDS 40.0%
ACAACATGCATAAGAGCCTT+AGG - chr1_4:41829587-41829606 None:intergenic 40.0%
AAGGCTCTTATGCATGTTGT+TGG + chr1_4:41829587-41829606 Msa0163560:CDS 40.0%
GCATGTTGTTGGAACCAAAA+TGG + chr1_4:41829598-41829617 Msa0163560:CDS 40.0%
CATATAGGTTGCATTTCACG+TGG + chr1_4:41829821-41829840 Msa0163560:intron 40.0%
ATTGCACGCTGTTTGTGTTA+TGG + chr1_4:41829906-41829925 Msa0163560:intron 40.0%
TTGCACGCTGTTTGTGTTAT+GGG + chr1_4:41829907-41829926 Msa0163560:intron 40.0%
GTTATGGGTCCTTTGTGTAA+TGG + chr1_4:41829922-41829941 Msa0163560:intron 40.0%
AAGAGTGGTCCATTACACAA+AGG - chr1_4:41829934-41829953 None:intergenic 40.0%
TATTTGTGTTACGGAACCTG+TGG - chr1_4:41829957-41829976 None:intergenic 40.0%
TCCAGTGAGTATGCAATGTT+GGG - chr1_4:41830015-41830034 None:intergenic 40.0%
ATGAACCAGTTGAGGATGAA+TGG - chr1_4:41830056-41830075 None:intergenic 40.0%
!! TCAGATTTGAACGAGTTGGT+AGG + chr1_4:41830118-41830137 Msa0163560:intron 40.0%
GTCGACAAGTTCTATGTTGA+AGG - chr1_4:41830218-41830237 None:intergenic 40.0%
GTGGATAGTTTAATCTGGAC+AGG - chr1_4:41830301-41830320 None:intergenic 40.0%
CTGAGGTGGATAGTTTAATC+TGG - chr1_4:41830306-41830325 None:intergenic 40.0%
!!! CCCACTTTTTCCTAATACTG+AGG - chr1_4:41830323-41830342 None:intergenic 40.0%
TATGCTGGACAACATGAGAA+AGG + chr1_4:41830353-41830372 Msa0163560:intron 40.0%
GTATTCTGCTATGCTATGCT+TGG + chr1_4:41830605-41830624 Msa0163560:intron 40.0%
!! TGCTATGCTTGGTCTTAGTT+AGG + chr1_4:41830616-41830635 Msa0163560:intron 40.0%
TTGCAATCCCAATTCTACGT+GGG + chr1_4:41830656-41830675 Msa0163560:intron 40.0%
!!! GTCTTTTGAGTTAGTGCTCT+TGG - chr1_4:41830715-41830734 None:intergenic 40.0%
TGTTAATTGCGGAAAACAGC+AGG + chr1_4:41830776-41830795 Msa0163560:intron 40.0%
TGTCAATTGCAGAAAACAGC+GGG + chr1_4:41830916-41830935 Msa0163560:intron 40.0%
CAAGGGCTATGCTAAAATAG+AGG - chr1_4:41831137-41831156 None:intergenic 40.0%
ACCTCTGTTGTAAAAGAGGA+AGG + chr1_4:41831172-41831191 Msa0163560:intron 40.0%
! ACCTTCCTCTTTTACAACAG+AGG - chr1_4:41831176-41831195 None:intergenic 40.0%
!! GGAAGGTGTTGATGAATATG+TGG + chr1_4:41831189-41831208 Msa0163560:intron 40.0%
GTGGCATGAATATTAGTGTG+TGG + chr1_4:41831208-41831227 Msa0163560:intron 40.0%
AAGATTCACCACAACCACAT+TGG - chr1_4:41831354-41831373 None:intergenic 40.0%
TCAACCATTTCCTTGCTTAG+AGG - chr1_4:41831401-41831420 None:intergenic 40.0%
TGGTATTTGCATGTTCACCA+TGG - chr1_4:41831480-41831499 None:intergenic 40.0%
! TTATTTTCCTCTGTACTCGC+AGG - chr1_4:41831525-41831544 None:intergenic 40.0%
TTGGCATGCTGATACTGAAA+TGG + chr1_4:41831552-41831571 Msa0163560:three_prime_UTR 40.0%
GACTTGTCGTAGGCTTATAG+CGG - chr1_4:41828603-41828622 None:intergenic 45.0%
!! AGGTGTTGTTGACTTGTCGT+AGG - chr1_4:41828613-41828632 None:intergenic 45.0%
TTTCCAACTCATGCGCCTAA+TGG + chr1_4:41828729-41828748 Msa0163560:five_prime_UTR 45.0%
CCTCGCGAAATCACGAAATA+CGG - chr1_4:41828861-41828880 None:intergenic 45.0%
! CGGTGCTAAATATAGCGAAC+AGG - chr1_4:41828887-41828906 None:intergenic 45.0%
CAGTACATGCCACATCATTC+GGG + chr1_4:41828961-41828980 Msa0163560:five_prime_UTR 45.0%
AATTGTTGTGCGTATGGCAG+TGG - chr1_4:41829046-41829065 None:intergenic 45.0%
GGTGGAAATTGTTGTGCGTA+TGG - chr1_4:41829052-41829071 None:intergenic 45.0%
! GAATTCGTTGGATTGGAGTC+TGG + chr1_4:41829079-41829098 Msa0163560:five_prime_UTR 45.0%
GCTGAGATTGAACGGTGTTT+GGG - chr1_4:41829189-41829208 None:intergenic 45.0%
TGCTGAGATTGAACGGTGTT+TGG - chr1_4:41829190-41829209 None:intergenic 45.0%
!! TTGACGCCGAGCTTTAAGAA+CGG - chr1_4:41829355-41829374 None:intergenic 45.0%
! AAACATTGATTGGGGGAGGA+GGG + chr1_4:41829744-41829763 Msa0163560:intron 45.0%
GTCCAGTGAGTATGCAATGT+TGG - chr1_4:41830016-41830035 None:intergenic 45.0%
ACATTGCATACTCACTGGAC+AGG + chr1_4:41830016-41830035 Msa0163560:intron 45.0%
! ATGTGCCATTCATCCTCAAC+TGG + chr1_4:41830048-41830067 Msa0163560:intron 45.0%
ACTCGTTCAAATCTGACCAC+AGG - chr1_4:41830114-41830133 None:intergenic 45.0%
GTGGTCAGATTTGAACGAGT+TGG + chr1_4:41830114-41830133 Msa0163560:intron 45.0%
!! CTAGCTTGGAGGATTTGTTG+CGG - chr1_4:41830147-41830166 None:intergenic 45.0%
CAAAGGTTCGTCATCTAGCT+TGG - chr1_4:41830161-41830180 None:intergenic 45.0%
CAAGCTAGATGACGAACCTT+TGG + chr1_4:41830159-41830178 Msa0163560:intron 45.0%
GAAGGAGATGGGATAGCATA+TGG - chr1_4:41830200-41830219 None:intergenic 45.0%
ATCTAATGATCCCGTGTGAG+AGG - chr1_4:41830411-41830430 None:intergenic 45.0%
TGATGTAGGATCCATGGTTG+CGG - chr1_4:41830500-41830519 None:intergenic 45.0%
GTTGCAATCCCAATTCTACG+TGG + chr1_4:41830655-41830674 Msa0163560:intron 45.0%
! GTGTCTTTTGAGCTGCTCTT+GGG - chr1_4:41830857-41830876 None:intergenic 45.0%
! AGTGTCTTTTGAGCTGCTCT+TGG - chr1_4:41830858-41830877 None:intergenic 45.0%
!! AAATAGTGTAGTGGACTCCG+TGG - chr1_4:41831019-41831038 None:intergenic 45.0%
! AATTTTGCCTGCGAGTACAG+AGG + chr1_4:41831515-41831534 Msa0163560:three_prime_UTR 45.0%
!! CTCTGAAGCTGATACTGACA+AGG + chr1_4:41831592-41831611 Msa0163560:three_prime_UTR 45.0%
GTTCCATTAGGCGCATGAGT+TGG - chr1_4:41828735-41828754 None:intergenic 50.0%
CCGTATTTCGTGATTTCGCG+AGG + chr1_4:41828858-41828877 Msa0163560:five_prime_UTR 50.0%
CGAAAACGTCCCGAATGATG+TGG - chr1_4:41828973-41828992 None:intergenic 50.0%
CCAACGAATTCGGAAGAAGG+TGG - chr1_4:41829070-41829089 None:intergenic 50.0%
CCACCTTCTTCCGAATTCGT+TGG + chr1_4:41829067-41829086 Msa0163560:five_prime_UTR 50.0%
!! GTTCGTCATTTTGGATCGCG+TGG - chr1_4:41829108-41829127 None:intergenic 50.0%
! GAAGTGTATTCTAGAGGCGG+CGG - chr1_4:41829311-41829330 None:intergenic 50.0%
GTCAAAGCTCGCGGATGTAA+TGG + chr1_4:41829370-41829389 Msa0163560:CDS 50.0%
! ACGCAAACATTGATTGGGGG+AGG + chr1_4:41829740-41829759 Msa0163560:intron 50.0%
! CAAACATTGATTGGGGGAGG+AGG + chr1_4:41829743-41829762 Msa0163560:intron 50.0%
!! GTAATGGACCACTCTTCCAC+AGG + chr1_4:41829938-41829957 Msa0163560:intron 50.0%
TTACGGAACCTGTGGAAGAG+TGG - chr1_4:41829949-41829968 None:intergenic 50.0%
GCCCAACATTGCATACTCAC+TGG + chr1_4:41830011-41830030 Msa0163560:intron 50.0%
!! AGGGCATTGAACATGACCTG+TGG + chr1_4:41830095-41830114 Msa0163560:intron 50.0%
! AGGTTCGTCATCTAGCTTGG+AGG - chr1_4:41830158-41830177 None:intergenic 50.0%
GCTAGATGACGAACCTTTGG+TGG + chr1_4:41830162-41830181 Msa0163560:intron 50.0%
GTTTAATCTGGACAGGCGAG+AGG - chr1_4:41830294-41830313 None:intergenic 50.0%
GTGGGTCCGACAAAATATGC+TGG + chr1_4:41830338-41830357 Msa0163560:intron 50.0%
TAATGATCCCGTGTGAGAGG+AGG - chr1_4:41830408-41830427 None:intergenic 50.0%
ATGGTTGCGGGTGGAAGATT+CGG - chr1_4:41830487-41830506 None:intergenic 50.0%
GATGTAGGATCCATGGTTGC+GGG - chr1_4:41830499-41830518 None:intergenic 50.0%
TGAGGGTGATGTAGGATCCA+TGG - chr1_4:41830506-41830525 None:intergenic 50.0%
!! CCTAATTGGTGATGGCGCTA+GGG - chr1_4:41830529-41830548 None:intergenic 50.0%
!! TCCTAATTGGTGATGGCGCT+AGG - chr1_4:41830530-41830549 None:intergenic 50.0%
GAACTCAGATCTGCCAGAAG+AGG - chr1_4:41831305-41831324 None:intergenic 50.0%
!! CCACATTGGCCTTTGGAGTT+TGG - chr1_4:41831340-41831359 None:intergenic 50.0%
CCAAACTCCAAAGGCCAATG+TGG + chr1_4:41831337-41831356 Msa0163560:CDS 50.0%
TCCAAAGGCCAATGTGGTTG+TGG + chr1_4:41831343-41831362 Msa0163560:CDS 50.0%
!! ACCACAACCACATTGGCCTT+TGG - chr1_4:41831347-41831366 None:intergenic 50.0%
TGCTTAGAGGCTCCAGTACT+TGG - chr1_4:41831388-41831407 None:intergenic 50.0%
! AGTACTGGAGCCTCTAAGCA+AGG + chr1_4:41831388-41831407 Msa0163560:CDS 50.0%
GGAGCCTCTAAGCAAGGAAA+TGG + chr1_4:41831394-41831413 Msa0163560:CDS 50.0%
! AGCAATGGGTTCCAAGGCGA+TGG + chr1_4:41829225-41829244 Msa0163560:exon 55.0%
GGCAGCGGAGAAGATAAGCA+CGG + chr1_4:41829252-41829271 Msa0163560:CDS 55.0%
AGCGGAGAAGATAAGCACGG+CGG + chr1_4:41829255-41829274 Msa0163560:CDS 55.0%
!! GGCGGCATCGGTTAACTCAA+CGG - chr1_4:41829293-41829312 None:intergenic 55.0%
! TATTCTAGAGGCGGCGGCAT+CGG - chr1_4:41829305-41829324 None:intergenic 55.0%
!! GTGTGCGAGAGTACCACCAA+AGG - chr1_4:41830178-41830197 None:intergenic 55.0%
ATCTGGACAGGCGAGAGGAA+TGG - chr1_4:41830289-41830308 None:intergenic 55.0%
ACATCCCACCTCCTCTCACA+CGG + chr1_4:41830397-41830416 Msa0163560:intron 55.0%
! TGCGGGTGGAAGATTCGGTT+TGG - chr1_4:41830482-41830501 None:intergenic 55.0%
GAATCTTCCACCCGCAACCA+TGG + chr1_4:41830486-41830505 Msa0163560:intron 55.0%
CCCTAGCGCCATCACCAATT+AGG + chr1_4:41830526-41830545 Msa0163560:intron 55.0%
GGGTTCCAAGGCGATGGCTA+TGG + chr1_4:41829231-41829250 Msa0163560:CDS 60.0%
CAAGGCGATGGCTATGGCAG+CGG + chr1_4:41829237-41829256 Msa0163560:CDS 60.0%
AGATAAGCACGGCGGTGCGA+CGG + chr1_4:41829263-41829282 Msa0163560:CDS 60.0%
AAGCTCGGCGTCAAAGCTCG+CGG + chr1_4:41829361-41829380 Msa0163560:CDS 60.0%
TCACGTGGGTGACTGTGCTG+AGG + chr1_4:41829836-41829855 Msa0163560:intron 60.0%
CATCCCACCTCCTCTCACAC+GGG + chr1_4:41830398-41830417 Msa0163560:intron 60.0%
GATCCCGTGTGAGAGGAGGT+GGG - chr1_4:41830404-41830423 None:intergenic 60.0%
TGATCCCGTGTGAGAGGAGG+TGG - chr1_4:41830405-41830424 None:intergenic 60.0%
GTAGGATCCATGGTTGCGGG+TGG - chr1_4:41830496-41830515 None:intergenic 60.0%
CGCTAGGGTGAGGGTGATGT+AGG - chr1_4:41830514-41830533 None:intergenic 60.0%
!! TGGTGATGGCGCTAGGGTGA+GGG - chr1_4:41830523-41830542 None:intergenic 60.0%
!! TTGGTGATGGCGCTAGGGTG+AGG - chr1_4:41830524-41830543 None:intergenic 60.0%
CGCTGCCATAGCCATCGCCT+TGG - chr1_4:41829239-41829258 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 41828600 41831726 41828600 ID=Msa0163560;Name=Msa0163560
chr1_4 mRNA 41828600 41831726 41828600 ID=Msa0163560-mRNA-1;Parent=Msa0163560;Name=Msa0163560-mRNA-1;_AED=0.01;_eAED=0.01;_QI=629|1|1|1|0.5|1|3|306|137
chr1_4 exon 41828600 41829418 41828600 ID=Msa0163560-mRNA-1:exon:12076;Parent=Msa0163560-mRNA-1
chr1_4 exon 41829525 41829642 41829525 ID=Msa0163560-mRNA-1:exon:12077;Parent=Msa0163560-mRNA-1
chr1_4 exon 41831315 41831726 41831315 ID=Msa0163560-mRNA-1:exon:12078;Parent=Msa0163560-mRNA-1
chr1_4 five_prime_UTR 41828600 41829228 41828600 ID=Msa0163560-mRNA-1:five_prime_utr;Parent=Msa0163560-mRNA-1
chr1_4 CDS 41829229 41829418 41829229 ID=Msa0163560-mRNA-1:cds;Parent=Msa0163560-mRNA-1
chr1_4 CDS 41829525 41829642 41829525 ID=Msa0163560-mRNA-1:cds;Parent=Msa0163560-mRNA-1
chr1_4 CDS 41831315 41831420 41831315 ID=Msa0163560-mRNA-1:cds;Parent=Msa0163560-mRNA-1
chr1_4 three_prime_UTR 41831421 41831726 41831421 ID=Msa0163560-mRNA-1:three_prime_utr;Parent=Msa0163560-mRNA-1
Gene Sequence

>Msa0163560

ATGGGTTCCAAGGCGATGGCTATGGCAGCGGAGAAGATAAGCACGGCGGTGCGACGGCAAGCCGTTGAGTTAACCGATGCCGCCGCCTCTAGAATACACTTCTTGCTTCAGCAACGGCAAAGACCGTTCTTAAAGCTCGGCGTCAAAGCTCGCGGATGTAATGGTTTATCTTACACGCTAAATTACGCAGATAAAAAAGAGAAATTTGATGAATTGGTTGAAGATAAAGGTGTTAAGATTTTGATTGATCCTAAGGCTCTTATGCATGTTGTTGGAACCAAAATGGATTTCGTTGATGATAAACTAAGATCTGAGTTCGTTTTCATTAATCCAAACTCCAAAGGCCAATGTGGTTGTGGTGAATCTTTCATGACAAAACCAAGTACTGGAGCCTCTAAGCAAGGAAATGGTTGA
Protein sequence

>Msa0163560

MGSKAMAMAAEKISTAVRRQAVELTDAAASRIHFLLQQRQRPFLKLGVKARGCNGLSYTLNYADKKEKFDELVEDKGVKILIDPKALMHVVGTKMDFVDDKLRSEFVFINPNSKGQCGCGESFMTKPSTGASKQGNG*