AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0024480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0024480 MtrunA17_Chr1g0178671 98.540 137 2 0 1 137 1 137 5.94e-100 282
Msa0024480 MtrunA17_Chr8g0383271 36.634 101 61 2 22 119 70 170 4.65e-18 75.9
Msa0024480 MtrunA17_Chr7g0266291 35.714 84 53 1 44 126 76 159 4.54e-14 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0024480 AT2G16710.1 75.188 133 32 1 2 134 3 134 4.43e-72 212
Msa0024480 AT2G16710.3 72.464 138 31 2 3 134 4 140 1.49e-69 206
Msa0024480 AT2G36260.1 69.725 109 32 1 17 125 1 108 1.03e-55 170
Msa0024480 AT2G16710.2 73.529 102 27 0 2 103 3 104 1.33e-51 159
Msa0024480 AT1G10500.1 32.407 108 70 2 19 123 69 176 8.10e-18 75.9
Msa0024480 AT5G03905.1 31.481 108 72 2 21 126 50 157 4.85e-13 62.8

Find 89 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGGTATGTTCGTCATTT+TGG 0.156614 1_1:+46894686 None:intergenic
ATCATCAACGAAATCCATTT+TGG 0.208042 1_1:+46894188 None:intergenic
CCACATTGGCCTTTGGAGTT+TGG 0.248774 1_1:+46892463 None:intergenic
AAAAGAGAAATTTGATGAAT+TGG 0.258711 1_1:-46894271 Msa0024480:CDS
ACTTCTCTATTTCTATTTCA+TGG 0.287553 1_1:-46895147 Msa0024480:five_prime_UTR
TCAACCATTTCCTTGCTTAG+AGG 0.300532 1_1:+46892402 None:intergenic
GCATGTTGTTGGAACCAAAA+TGG 0.312759 1_1:-46894202 Msa0024480:CDS
TGCTTAGAGGCTCCAGTACT+TGG 0.316007 1_1:+46892415 None:intergenic
TTTCCAACTCATGCGCCTAA+TGG 0.325435 1_1:-46895079 Msa0024480:five_prime_UTR
TGCTGAGATTGAACGGTGTT+TGG 0.336566 1_1:+46894613 None:intergenic
CTCTATTTCTATTTCATGGT+TGG 0.350103 1_1:-46895143 Msa0024480:five_prime_UTR
GTGGATACTGTATAATTTGT+AGG 0.368896 1_1:-46892122 Msa0024480:three_prime_UTR
TCAGTACATGCCACATCATT+CGG 0.376103 1_1:-46894840 Msa0024480:five_prime_UTR
TTTAGTCATTTGAACATGTA+AGG 0.381086 1_1:+46894658 None:intergenic
TTCTTCCGAATTCGTTGGAT+TGG 0.383312 1_1:-46894728 Msa0024480:five_prime_UTR
GATGAATTGGTTGAAGATAA+AGG 0.389292 1_1:-46894258 Msa0024480:CDS
ACCACAACCACATTGGCCTT+TGG 0.397048 1_1:+46892456 None:intergenic
GCTGAGATTGAACGGTGTTT+GGG 0.419964 1_1:+46894614 None:intergenic
AGACTCCAATCCAACGAATT+CGG 0.422958 1_1:+46894723 None:intergenic
AATGACTAAATTAACCTCAT+AGG 0.422962 1_1:-46894645 Msa0024480:five_prime_UTR
AAAATAGATGATGGTATTCA+AGG 0.433143 1_1:+46895046 None:intergenic
GAATTCGTTGGATTGGAGTC+TGG 0.444757 1_1:-46894721 Msa0024480:five_prime_UTR
TTTCGTAATTAACAAGCAAT+GGG 0.449583 1_1:-46894589 Msa0024480:exon
TTGGCATGCTGATACTGAAA+TGG 0.458736 1_1:-46892248 Msa0024480:three_prime_UTR
GGAGCCTCTAAGCAAGGAAA+TGG 0.461818 1_1:-46892406 Msa0024480:CDS
AAGGCTCTTATGCATGTTGT+TGG 0.463150 1_1:-46894213 Msa0024480:CDS
ATTTCGTAATTAACAAGCAA+TGG 0.463321 1_1:-46894590 Msa0024480:exon
TTTGGGGATTTATACCTATG+AGG 0.467243 1_1:+46894631 None:intergenic
CAGTACATGCCACATCATTC+GGG 0.468012 1_1:-46894839 Msa0024480:five_prime_UTR
CCACCTTCTTCCGAATTCGT+TGG 0.476252 1_1:-46894733 Msa0024480:five_prime_UTR
CGGTGCTAAATATAGCGAAC+AGG 0.479621 1_1:+46894916 None:intergenic
ACAACATGCATAAGAGCCTT+AGG 0.481683 1_1:+46894216 None:intergenic
AGGAAATTACATAGAGAAGA+AGG 0.487338 1_1:+46895188 None:intergenic
TGAACATGTAAGGACATGTA+AGG 0.491010 1_1:+46894668 None:intergenic
CAAGGTCCATGGGTTCCATT+AGG 0.493368 1_1:+46895064 None:intergenic
ATGATGGTATTCAAGGTCCA+TGG 0.498566 1_1:+46895053 None:intergenic
ATTTCGTTGATGATAAACTA+AGG 0.504621 1_1:-46894179 Msa0024480:intron
GTCAAAGCTCGCGGATGTAA+TGG 0.505977 1_1:-46894430 Msa0024480:CDS
TTCATGACAAAACCAAGTAC+TGG 0.509644 1_1:-46892427 Msa0024480:CDS
GGGTTCCAAGGCGATGGCTA+TGG 0.513236 1_1:-46894569 Msa0024480:CDS
TCCAAAGGCCAATGTGGTTG+TGG 0.524509 1_1:-46892457 Msa0024480:CDS
CCTCGCGAAATCACGAAATA+CGG 0.525892 1_1:+46894942 None:intergenic
AATCCAACGAATTCGGAAGA+AGG 0.529950 1_1:+46894730 None:intergenic
TTCATTAATCCAAACTCCAA+AGG 0.536589 1_1:-46892472 Msa0024480:CDS
TGCTGCCATAGCCATCGCCT+TGG 0.537499 1_1:+46894564 None:intergenic
GTTGTCAGAAGAAGATCTAG+AGG 0.539767 1_1:+46895168 None:intergenic
ACGAAATTGCTGAGATTGAA+CGG 0.542457 1_1:+46894606 None:intergenic
TATTCTAGAGGCGGCGGCAT+CGG 0.552495 1_1:+46894498 None:intergenic
CAAAGACCGTTCTTAAAGCT+CGG 0.558956 1_1:-46894454 Msa0024480:CDS
TTAACAAGCAATGGGTTCCA+AGG 0.561441 1_1:-46894581 Msa0024480:exon
TACACTTCTTGCTTCAGCAA+CGG 0.562185 1_1:-46894477 Msa0024480:CDS
GTTGAAATTATCATTCAACT+AGG 0.573251 1_1:-46892384 Msa0024480:exon
AGCAATGGGTTCCAAGGCGA+TGG 0.573401 1_1:-46894575 Msa0024480:exon
GTTCCATTAGGCGCATGAGT+TGG 0.574673 1_1:+46895076 None:intergenic
AGATAAGCACGGCGGTGCGA+CGG 0.576266 1_1:-46894537 Msa0024480:CDS
AATTGTTGTGCGTATGGCAG+TGG 0.579932 1_1:+46894757 None:intergenic
TGATGGTATTCAAGGTCCAT+GGG 0.580246 1_1:+46895054 None:intergenic
GGTGGAAATTGTTGTGCGTA+TGG 0.580291 1_1:+46894751 None:intergenic
GGCGGCATCGGTTAACTCAA+CGG 0.586444 1_1:+46894510 None:intergenic
TCTGTGGCTATGAATTTCAG+AGG 0.588281 1_1:-46895024 Msa0024480:five_prime_UTR
CTCTGAAGCTGATACTGACA+AGG 0.590200 1_1:-46892208 Msa0024480:three_prime_UTR
TAGCCACAGAAAATAGATGA+TGG 0.591925 1_1:+46895037 None:intergenic
CATGCGCCTAATGGAACCCA+TGG 0.592755 1_1:-46895070 Msa0024480:five_prime_UTR
CCAACGAATTCGGAAGAAGG+TGG 0.596750 1_1:+46894733 None:intergenic
AGGTGTTGTTGACTTGTCGT+AGG 0.597446 1_1:+46895211 None:intergenic
AAATTACATAGAGAAGAAGG+AGG 0.601528 1_1:+46895191 None:intergenic
GAAGTGTATTCTAGAGGCGG+CGG 0.606928 1_1:+46894492 None:intergenic
TTTAATTTAATGAGAAAGAG+CGG 0.607923 1_1:+46894896 None:intergenic
CTGAGATTGAACGGTGTTTG+GGG 0.614313 1_1:+46894615 None:intergenic
TTGTCGTAGGCTAATAGCGG+TGG 0.620718 1_1:+46895224 None:intergenic
AAATTACAGAAATTCAATTG+TGG 0.623213 1_1:+46892303 None:intergenic
AGTACTGGAGCCTCTAAGCA+AGG 0.624921 1_1:-46892412 Msa0024480:CDS
AAGATTCACCACAACCACAT+TGG 0.628045 1_1:+46892449 None:intergenic
CCAAACTCCAAAGGCCAATG+TGG 0.629156 1_1:-46892463 Msa0024480:CDS
CCGTATTTCGTGATTTCGCG+AGG 0.633478 1_1:-46894942 Msa0024480:five_prime_UTR
GAACATGTAAGGACATGTAA+GGG 0.635484 1_1:+46894669 None:intergenic
CGAAAACGTCCCGAATGATG+TGG 0.636787 1_1:+46894830 None:intergenic
CAAGAAGTGTATTCTAGAGG+CGG 0.641303 1_1:+46894489 None:intergenic
TGGTATTTGCATGTTCACCA+TGG 0.645858 1_1:+46892323 None:intergenic
AAGCAAGAAGTGTATTCTAG+AGG 0.646366 1_1:+46894486 None:intergenic
GGCAGCAGAGAAGATAAGCA+CGG 0.647942 1_1:-46894548 Msa0024480:CDS
TTGACGCCGAGCTTTAAGAA+CGG 0.652738 1_1:+46894448 None:intergenic
AAGCTCGGCGTCAAAGCTCG+CGG 0.657327 1_1:-46894439 Msa0024480:CDS
TCTTACACGCTAAATTACGC+AGG 0.659336 1_1:-46894403 Msa0024480:intron
GACTTGTCGTAGGCTAATAG+CGG 0.662488 1_1:+46895221 None:intergenic
GTTTGAAAACATGAGTGTGG+TGG 0.682217 1_1:+46894969 None:intergenic
TCAGTTTGAAAACATGAGTG+TGG 0.728519 1_1:+46894966 None:intergenic
AGCAGAGAAGATAAGCACGG+CGG 0.734036 1_1:-46894545 Msa0024480:CDS
TTATTGAAGAGAGAATCGAA+AGG 0.762981 1_1:+46894996 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATTAAAATTAAAAA+AGG - chr1_1:46892438-46892457 Msa0024480:CDS 0.0%
!! ATTAAACGAAAATTTAAAGA+GGG + chr1_1:46892953-46892972 None:intergenic 15.0%
!! AATTAAACGAAAATTTAAAG+AGG + chr1_1:46892954-46892973 None:intergenic 15.0%
!!! TTTAATTTAATGAGAAAGAG+CGG + chr1_1:46892424-46892443 None:intergenic 20.0%
!!! GTATTGATTTTAAGATTTGT+TGG - chr1_1:46893005-46893024 Msa0024480:intron 20.0%
!! AAAAGAGAAATTTGATGAAT+TGG - chr1_1:46893046-46893065 Msa0024480:intron 20.0%
!!! TTCTTTGTTCAATTTTGTTT+TGG - chr1_1:46893199-46893218 Msa0024480:intron 20.0%
!!! TTTTTTTTCCCCAATAAATA+AGG + chr1_1:46893238-46893257 None:intergenic 20.0%
!! TAATTATATGATGAGGTTTA+TGG + chr1_1:46893759-46893778 None:intergenic 20.0%
!!! TTGCTTATGTTTTTTCTTTT+CGG - chr1_1:46894771-46894790 Msa0024480:five_prime_UTR 20.0%
!! AAATTACAGAAATTCAATTG+TGG + chr1_1:46895017-46895036 None:intergenic 20.0%
!! ATTCATGTAAAAAACTATTG+TGG - chr1_1:46895176-46895195 Msa0024480:five_prime_UTR 20.0%
!!! ACTTCTCTATTTCTATTTCA+TGG - chr1_1:46892170-46892189 Msa0024480:three_prime_UTR 25.0%
!! AAAATAGATGATGGTATTCA+AGG + chr1_1:46892274-46892293 None:intergenic 25.0%
! TTAAAAAAGGAAAATGCAAG+TGG - chr1_1:46892451-46892470 Msa0024480:CDS 25.0%
! TAAAAAAGGAAAATGCAAGT+GGG - chr1_1:46892452-46892471 Msa0024480:CDS 25.0%
! TTTAGTCATTTGAACATGTA+AGG + chr1_1:46892662-46892681 None:intergenic 25.0%
! AATGACTAAATTAACCTCAT+AGG - chr1_1:46892672-46892691 Msa0024480:intron 25.0%
! ATTTCGTAATTAACAAGCAA+TGG - chr1_1:46892727-46892746 Msa0024480:intron 25.0%
! TTTCGTAATTAACAAGCAAT+GGG - chr1_1:46892728-46892747 Msa0024480:intron 25.0%
!!! TTGATTTTAAGATTTGTTGG+TGG - chr1_1:46893008-46893027 Msa0024480:intron 25.0%
!!! TAAGATTTTGATTGATCCTA+AGG - chr1_1:46893085-46893104 Msa0024480:intron 25.0%
!!! ATTTCGTTGATGATAAACTA+AGG - chr1_1:46893138-46893157 Msa0024480:intron 25.0%
!! TCTTGTTCAACCTTATTTAT+TGG - chr1_1:46893225-46893244 Msa0024480:intron 25.0%
!! CTTGTTCAACCTTATTTATT+GGG - chr1_1:46893226-46893245 Msa0024480:intron 25.0%
!! TTGTTCAACCTTATTTATTG+GGG - chr1_1:46893227-46893246 Msa0024480:intron 25.0%
! AAAAAAACGCAAACATTGAT+TGG - chr1_1:46893251-46893270 Msa0024480:intron 25.0%
!! AAAAAACGCAAACATTGATT+GGG - chr1_1:46893252-46893271 Msa0024480:intron 25.0%
! AGTATGTCATAAATCTCATT+TGG - chr1_1:46893956-46893975 Msa0024480:intron 25.0%
! AAGTAATGCATATTCCTAAT+TGG + chr1_1:46894060-46894079 None:intergenic 25.0%
! TAGGAATATGCATTACTTAA+AGG - chr1_1:46894062-46894081 Msa0024480:intron 25.0%
! AGGAATATGCATTACTTAAA+GGG - chr1_1:46894063-46894082 Msa0024480:intron 25.0%
! GTTGAAATTATCATTCAACT+AGG - chr1_1:46894933-46894952 Msa0024480:five_prime_UTR 25.0%
!! TCTTTCTTTTATGATTTCCA+TGG - chr1_1:46894977-46894996 Msa0024480:five_prime_UTR 25.0%
! AGAGGAAAATAAAAAAGCTT+TGG - chr1_1:46895050-46895069 Msa0024480:five_prime_UTR 25.0%
AAATTACATAGAGAAGAAGG+AGG + chr1_1:46892129-46892148 None:intergenic 30.0%
AGGAAATTACATAGAGAAGA+AGG + chr1_1:46892132-46892151 None:intergenic 30.0%
!! CTCTATTTCTATTTCATGGT+TGG - chr1_1:46892174-46892193 Msa0024480:three_prime_UTR 30.0%
! TGAGTTGGAAAGAAAAACTA+TGG + chr1_1:46892229-46892248 None:intergenic 30.0%
! ATACCATCATCTATTTTCTG+TGG - chr1_1:46892277-46892296 Msa0024480:three_prime_UTR 30.0%
TTATTGAAGAGAGAATCGAA+AGG + chr1_1:46892324-46892343 None:intergenic 30.0%
GATGAATTGGTTGAAGATAA+AGG - chr1_1:46893059-46893078 Msa0024480:intron 30.0%
! ATCATCAACGAAATCCATTT+TGG + chr1_1:46893132-46893151 None:intergenic 30.0%
! AAAAACGCAAACATTGATTG+GGG - chr1_1:46893253-46893272 Msa0024480:intron 30.0%
TCAGTAAACCTTACTAGTTA+AGG + chr1_1:46893304-46893323 None:intergenic 30.0%
ATTATGTAATGAACCAGTTG+AGG + chr1_1:46893581-46893600 None:intergenic 30.0%
! TTACATAATTTGCATGCTAC+AGG - chr1_1:46893592-46893611 Msa0024480:intron 30.0%
! TACATAATTTGCATGCTACA+GGG - chr1_1:46893593-46893612 Msa0024480:intron 30.0%
CCTCATCATATAATTATACC+CGG - chr1_1:46893763-46893782 Msa0024480:intron 30.0%
! TATTTTACCCACGTAGAATT+GGG + chr1_1:46894183-46894202 None:intergenic 30.0%
! TACATCAGTGTTGTTAATTG+CGG - chr1_1:46894282-46894301 Msa0024480:intron 30.0%
ATTAGTTACTGCTTGATCTT+TGG - chr1_1:46894402-46894421 Msa0024480:intron 30.0%
AATATGCTACAATGCTATAG+CGG - chr1_1:46894469-46894488 Msa0024480:CDS 30.0%
TTGTACTAAATAGCATACCA+CGG - chr1_1:46894516-46894535 Msa0024480:CDS 30.0%
! GTCTAAAGCTTTATGTACAA+GGG + chr1_1:46894671-46894690 None:intergenic 30.0%
AAAAAACATAAGCAAGTAGC+AGG + chr1_1:46894766-46894785 None:intergenic 30.0%
! AAATACTATTTCTCCTCTTC+TGG - chr1_1:46894806-46894825 Msa0024480:five_prime_UTR 30.0%
TTCATTAATCCAAACTCCAA+AGG - chr1_1:46894845-46894864 Msa0024480:five_prime_UTR 30.0%
! GTGGATACTGTATAATTTGT+AGG - chr1_1:46895195-46895214 Msa0024480:five_prime_UTR 30.0%
TAGCCACAGAAAATAGATGA+TGG + chr1_1:46892283-46892302 None:intergenic 35.0%
TCAGTTTGAAAACATGAGTG+TGG + chr1_1:46892354-46892373 None:intergenic 35.0%
!! AAGGGTATGTTCGTCATTTT+GGG + chr1_1:46892633-46892652 None:intergenic 35.0%
!! TAAGGGTATGTTCGTCATTT+TGG + chr1_1:46892634-46892653 None:intergenic 35.0%
GAACATGTAAGGACATGTAA+GGG + chr1_1:46892651-46892670 None:intergenic 35.0%
TGAACATGTAAGGACATGTA+AGG + chr1_1:46892652-46892671 None:intergenic 35.0%
TTTGGGGATTTATACCTATG+AGG + chr1_1:46892689-46892708 None:intergenic 35.0%
ACGAAATTGCTGAGATTGAA+CGG + chr1_1:46892714-46892733 None:intergenic 35.0%
! AAGCAAGAAGTGTATTCTAG+AGG + chr1_1:46892834-46892853 None:intergenic 35.0%
! AAAACGCAAACATTGATTGG+GGG - chr1_1:46893254-46893273 Msa0024480:intron 35.0%
ACTAGTTAAGGCTTCAAACA+TGG + chr1_1:46893292-46893311 None:intergenic 35.0%
! GTTTGAAGCCTTAACTAGTA+AGG - chr1_1:46893293-46893312 Msa0024480:intron 35.0%
ATATAGGTTGCATTTCACGT+GGG - chr1_1:46893339-46893358 Msa0024480:intron 35.0%
ATTGCACGCTATTTGTGTTA+CGG + chr1_1:46893483-46893502 None:intergenic 35.0%
AGTTCTATGTTGAAGGAGAT+GGG + chr1_1:46893728-46893747 None:intergenic 35.0%
AAGTTCTATGTTGAAGGAGA+TGG + chr1_1:46893729-46893748 None:intergenic 35.0%
CCGGGTATAATTATATGATG+AGG + chr1_1:46893766-46893785 None:intergenic 35.0%
! GTATTTATTCGGTTTGAACC+GGG + chr1_1:46893784-46893803 None:intergenic 35.0%
! GGTATTTATTCGGTTTGAAC+CGG + chr1_1:46893785-46893804 None:intergenic 35.0%
CGAGAGGAATGGTATTTATT+CGG + chr1_1:46893795-46893814 None:intergenic 35.0%
AAACTATCCACCTCAGTATT+AGG - chr1_1:46893827-46893846 Msa0024480:intron 35.0%
!! ACTTTTTCCTAATACTGAGG+TGG + chr1_1:46893837-46893856 None:intergenic 35.0%
ACCTCAGTATTAGGAAAAAG+TGG - chr1_1:46893836-46893855 Msa0024480:intron 35.0%
CCTCAGTATTAGGAAAAAGT+GGG - chr1_1:46893837-46893856 Msa0024480:intron 35.0%
! TGTTGTCCAGCATATTTTGT+CGG + chr1_1:46893864-46893883 None:intergenic 35.0%
TGCATATTCCTAATTGGTGA+TGG + chr1_1:46894054-46894073 None:intergenic 35.0%
GATGAAATACTCATGACATC+TGG - chr1_1:46894094-46894113 Msa0024480:intron 35.0%
! GTATTTTACCCACGTAGAAT+TGG + chr1_1:46894184-46894203 None:intergenic 35.0%
!!! TCTTTTGAGTTAGTGCTCTT+GGG + chr1_1:46894231-46894250 None:intergenic 35.0%
TTGTCAATTGCAGAAAACAG+CGG - chr1_1:46894432-46894451 Msa0024480:CDS 35.0%
TAGACGCTAAAATAGTGTAG+TGG + chr1_1:46894545-46894564 None:intergenic 35.0%
AGAGCAGTAGATTCAAATAG+AGG + chr1_1:46894604-46894623 None:intergenic 35.0%
TTGAATCTACTGCTCTTGAT+CGG - chr1_1:46894608-46894627 Msa0024480:five_prime_UTR 35.0%
! GGTCTAAAGCTTTATGTACA+AGG + chr1_1:46894672-46894691 None:intergenic 35.0%
TTAGACCTCTGTTGTAAAAG+AGG - chr1_1:46894685-46894704 Msa0024480:five_prime_UTR 35.0%
TTCATGACAAAACCAAGTAC+TGG - chr1_1:46894890-46894909 Msa0024480:five_prime_UTR 35.0%
GTTGTCAGAAGAAGATCTAG+AGG + chr1_1:46892152-46892171 None:intergenic 40.0%
TGATGGTATTCAAGGTCCAT+GGG + chr1_1:46892266-46892285 None:intergenic 40.0%
ATGATGGTATTCAAGGTCCA+TGG + chr1_1:46892267-46892286 None:intergenic 40.0%
TCTGTGGCTATGAATTTCAG+AGG - chr1_1:46892293-46892312 Msa0024480:three_prime_UTR 40.0%
GTTTGAAAACATGAGTGTGG+TGG + chr1_1:46892351-46892370 None:intergenic 40.0%
TCAGTACATGCCACATCATT+CGG - chr1_1:46892477-46892496 Msa0024480:CDS 40.0%
AATCCAACGAATTCGGAAGA+AGG + chr1_1:46892590-46892609 None:intergenic 40.0%
TTCTTCCGAATTCGTTGGAT+TGG - chr1_1:46892589-46892608 Msa0024480:intron 40.0%
AGACTCCAATCCAACGAATT+CGG + chr1_1:46892597-46892616 None:intergenic 40.0%
! TTAACAAGCAATGGGTTCCA+AGG - chr1_1:46892736-46892755 Msa0024480:intron 40.0%
! CAAGAAGTGTATTCTAGAGG+CGG + chr1_1:46892831-46892850 None:intergenic 40.0%
TACACTTCTTGCTTCAGCAA+CGG - chr1_1:46892840-46892859 Msa0024480:intron 40.0%
CAAAGACCGTTCTTAAAGCT+CGG - chr1_1:46892863-46892882 Msa0024480:intron 40.0%
TCTTACACGCTAAATTACGC+AGG - chr1_1:46892914-46892933 Msa0024480:intron 40.0%
ACAACATGCATAAGAGCCTT+AGG + chr1_1:46893104-46893123 None:intergenic 40.0%
AAGGCTCTTATGCATGTTGT+TGG - chr1_1:46893104-46893123 Msa0024480:intron 40.0%
GCATGTTGTTGGAACCAAAA+TGG - chr1_1:46893115-46893134 Msa0024480:intron 40.0%
GTTTACTGAGTGATTGAAGC+CGG - chr1_1:46893315-46893334 Msa0024480:intron 40.0%
GTGAAATGCAACCTATATGC+CGG + chr1_1:46893337-46893356 None:intergenic 40.0%
CATATAGGTTGCATTTCACG+TGG - chr1_1:46893338-46893357 Msa0024480:intron 40.0%
ATTGCACGCTGTTTGTGTTA+TGG - chr1_1:46893423-46893442 Msa0024480:intron 40.0%
TTGCACGCTGTTTGTGTTAT+GGG - chr1_1:46893424-46893443 Msa0024480:intron 40.0%
GTTATGGGTCCTTTGTGTAA+TGG - chr1_1:46893439-46893458 Msa0024480:intron 40.0%
AAGAGTGGTCCATTACACAA+AGG + chr1_1:46893451-46893470 None:intergenic 40.0%
TATTTGTGTTACGGAACCTG+TGG + chr1_1:46893474-46893493 None:intergenic 40.0%
TCCAGTGAGTATGCAATGTT+GGG + chr1_1:46893532-46893551 None:intergenic 40.0%
ATGAACCAGTTGAGGATGAA+TGG + chr1_1:46893573-46893592 None:intergenic 40.0%
!! TCAGATTTGAACGAGTTGGT+AGG - chr1_1:46893635-46893654 Msa0024480:intron 40.0%
GTCGACAAGTTCTATGTTGA+AGG + chr1_1:46893735-46893754 None:intergenic 40.0%
GTGGATAGTTTAATCTGGAC+AGG + chr1_1:46893818-46893837 None:intergenic 40.0%
CTGAGGTGGATAGTTTAATC+TGG + chr1_1:46893823-46893842 None:intergenic 40.0%
!!! CCCACTTTTTCCTAATACTG+AGG + chr1_1:46893840-46893859 None:intergenic 40.0%
TATGCTGGACAACATGAGAA+AGG - chr1_1:46893870-46893889 Msa0024480:intron 40.0%
GTATTCTGCTATGCTATGCT+TGG - chr1_1:46894122-46894141 Msa0024480:intron 40.0%
!! TGCTATGCTTGGTCTTAGTT+AGG - chr1_1:46894133-46894152 Msa0024480:intron 40.0%
TTGCAATCCCAATTCTACGT+GGG - chr1_1:46894173-46894192 Msa0024480:intron 40.0%
!!! GTCTTTTGAGTTAGTGCTCT+TGG + chr1_1:46894232-46894251 None:intergenic 40.0%
TGTTAATTGCGGAAAACAGC+AGG - chr1_1:46894293-46894312 Msa0024480:intron 40.0%
TGTCAATTGCAGAAAACAGC+GGG - chr1_1:46894433-46894452 Msa0024480:CDS 40.0%
CAAGGGCTATGCTAAAATAG+AGG + chr1_1:46894654-46894673 None:intergenic 40.0%
ACCTCTGTTGTAAAAGAGGA+AGG - chr1_1:46894689-46894708 Msa0024480:five_prime_UTR 40.0%
! ACCTTCCTCTTTTACAACAG+AGG + chr1_1:46894693-46894712 None:intergenic 40.0%
!! GGAAGGTGTTGATGAATATG+TGG - chr1_1:46894706-46894725 Msa0024480:five_prime_UTR 40.0%
GTGGCATGAATATTAGTGTG+TGG - chr1_1:46894725-46894744 Msa0024480:five_prime_UTR 40.0%
AAGATTCACCACAACCACAT+TGG + chr1_1:46894871-46894890 None:intergenic 40.0%
TCAACCATTTCCTTGCTTAG+AGG + chr1_1:46894918-46894937 None:intergenic 40.0%
TGGTATTTGCATGTTCACCA+TGG + chr1_1:46894997-46895016 None:intergenic 40.0%
! TTATTTTCCTCTGTACTCGC+AGG + chr1_1:46895042-46895061 None:intergenic 40.0%
TTGGCATGCTGATACTGAAA+TGG - chr1_1:46895069-46895088 Msa0024480:five_prime_UTR 40.0%
GACTTGTCGTAGGCTAATAG+CGG + chr1_1:46892099-46892118 None:intergenic 45.0%
!! AGGTGTTGTTGACTTGTCGT+AGG + chr1_1:46892109-46892128 None:intergenic 45.0%
TTTCCAACTCATGCGCCTAA+TGG - chr1_1:46892238-46892257 Msa0024480:three_prime_UTR 45.0%
CCTCGCGAAATCACGAAATA+CGG + chr1_1:46892378-46892397 None:intergenic 45.0%
! CGGTGCTAAATATAGCGAAC+AGG + chr1_1:46892404-46892423 None:intergenic 45.0%
CAGTACATGCCACATCATTC+GGG - chr1_1:46892478-46892497 Msa0024480:CDS 45.0%
AATTGTTGTGCGTATGGCAG+TGG + chr1_1:46892563-46892582 None:intergenic 45.0%
GGTGGAAATTGTTGTGCGTA+TGG + chr1_1:46892569-46892588 None:intergenic 45.0%
! GAATTCGTTGGATTGGAGTC+TGG - chr1_1:46892596-46892615 Msa0024480:intron 45.0%
CTGAGATTGAACGGTGTTTG+GGG + chr1_1:46892705-46892724 None:intergenic 45.0%
GCTGAGATTGAACGGTGTTT+GGG + chr1_1:46892706-46892725 None:intergenic 45.0%
TGCTGAGATTGAACGGTGTT+TGG + chr1_1:46892707-46892726 None:intergenic 45.0%
!! TTGACGCCGAGCTTTAAGAA+CGG + chr1_1:46892872-46892891 None:intergenic 45.0%
! AAACATTGATTGGGGGAGGA+GGG - chr1_1:46893261-46893280 Msa0024480:intron 45.0%
AGTGATTGAAGCCGGCATAT+AGG - chr1_1:46893323-46893342 Msa0024480:intron 45.0%
GTCCAGTGAGTATGCAATGT+TGG + chr1_1:46893533-46893552 None:intergenic 45.0%
ACATTGCATACTCACTGGAC+AGG - chr1_1:46893533-46893552 Msa0024480:intron 45.0%
! ATGTGCCATTCATCCTCAAC+TGG - chr1_1:46893565-46893584 Msa0024480:intron 45.0%
ACTCGTTCAAATCTGACCAC+AGG + chr1_1:46893631-46893650 None:intergenic 45.0%
GTGGTCAGATTTGAACGAGT+TGG - chr1_1:46893631-46893650 Msa0024480:intron 45.0%
!! CTAGCTTGGAGGATTTGTTG+CGG + chr1_1:46893664-46893683 None:intergenic 45.0%
CAAAGGTTCGTCATCTAGCT+TGG + chr1_1:46893678-46893697 None:intergenic 45.0%
CAAGCTAGATGACGAACCTT+TGG - chr1_1:46893676-46893695 Msa0024480:intron 45.0%
GAAGGAGATGGGATAGCATA+TGG + chr1_1:46893717-46893736 None:intergenic 45.0%
ATCTAATGATCCCGTGTGAG+AGG + chr1_1:46893928-46893947 None:intergenic 45.0%
TGATGTAGGATCCATGGTTG+CGG + chr1_1:46894017-46894036 None:intergenic 45.0%
GTTGCAATCCCAATTCTACG+TGG - chr1_1:46894172-46894191 Msa0024480:intron 45.0%
! GTGTCTTTTGAGCTGCTCTT+GGG + chr1_1:46894374-46894393 None:intergenic 45.0%
! AGTGTCTTTTGAGCTGCTCT+TGG + chr1_1:46894375-46894394 None:intergenic 45.0%
!! AAATAGTGTAGTGGACTCCG+TGG + chr1_1:46894536-46894555 None:intergenic 45.0%
! AATTTTGCCTGCGAGTACAG+AGG - chr1_1:46895032-46895051 Msa0024480:five_prime_UTR 45.0%
!! CTCTGAAGCTGATACTGACA+AGG - chr1_1:46895109-46895128 Msa0024480:five_prime_UTR 45.0%
GTTCCATTAGGCGCATGAGT+TGG + chr1_1:46892244-46892263 None:intergenic 50.0%
CAAGGTCCATGGGTTCCATT+AGG + chr1_1:46892256-46892275 None:intergenic 50.0%
CCGTATTTCGTGATTTCGCG+AGG - chr1_1:46892375-46892394 Msa0024480:three_prime_UTR 50.0%
CGAAAACGTCCCGAATGATG+TGG + chr1_1:46892490-46892509 None:intergenic 50.0%
CCAACGAATTCGGAAGAAGG+TGG + chr1_1:46892587-46892606 None:intergenic 50.0%
CCACCTTCTTCCGAATTCGT+TGG - chr1_1:46892584-46892603 Msa0024480:intron 50.0%
GGCAGCAGAGAAGATAAGCA+CGG - chr1_1:46892769-46892788 Msa0024480:intron 50.0%
AGCAGAGAAGATAAGCACGG+CGG - chr1_1:46892772-46892791 Msa0024480:intron 50.0%
! GAAGTGTATTCTAGAGGCGG+CGG + chr1_1:46892828-46892847 None:intergenic 50.0%
GTCAAAGCTCGCGGATGTAA+TGG - chr1_1:46892887-46892906 Msa0024480:intron 50.0%
! ACGCAAACATTGATTGGGGG+AGG - chr1_1:46893257-46893276 Msa0024480:intron 50.0%
! CAAACATTGATTGGGGGAGG+AGG - chr1_1:46893260-46893279 Msa0024480:intron 50.0%
!! GTAATGGACCACTCTTCCAC+AGG - chr1_1:46893455-46893474 Msa0024480:intron 50.0%
TTACGGAACCTGTGGAAGAG+TGG + chr1_1:46893466-46893485 None:intergenic 50.0%
GCCCAACATTGCATACTCAC+TGG - chr1_1:46893528-46893547 Msa0024480:intron 50.0%
!! AGGGCATTGAACATGACCTG+TGG - chr1_1:46893612-46893631 Msa0024480:intron 50.0%
! AGGTTCGTCATCTAGCTTGG+AGG + chr1_1:46893675-46893694 None:intergenic 50.0%
GCTAGATGACGAACCTTTGG+TGG - chr1_1:46893679-46893698 Msa0024480:intron 50.0%
GTTTAATCTGGACAGGCGAG+AGG + chr1_1:46893811-46893830 None:intergenic 50.0%
GTGGGTCCGACAAAATATGC+TGG - chr1_1:46893855-46893874 Msa0024480:intron 50.0%
TAATGATCCCGTGTGAGAGG+AGG + chr1_1:46893925-46893944 None:intergenic 50.0%
ATGGTTGCGGGTGGAAGATT+CGG + chr1_1:46894004-46894023 None:intergenic 50.0%
GATGTAGGATCCATGGTTGC+GGG + chr1_1:46894016-46894035 None:intergenic 50.0%
TGAGGGTGATGTAGGATCCA+TGG + chr1_1:46894023-46894042 None:intergenic 50.0%
!! CCTAATTGGTGATGGCGCTA+GGG + chr1_1:46894046-46894065 None:intergenic 50.0%
!! TCCTAATTGGTGATGGCGCT+AGG + chr1_1:46894047-46894066 None:intergenic 50.0%
GAACTCAGATCTGCCAGAAG+AGG + chr1_1:46894822-46894841 None:intergenic 50.0%
!! CCACATTGGCCTTTGGAGTT+TGG + chr1_1:46894857-46894876 None:intergenic 50.0%
CCAAACTCCAAAGGCCAATG+TGG - chr1_1:46894854-46894873 Msa0024480:five_prime_UTR 50.0%
TCCAAAGGCCAATGTGGTTG+TGG - chr1_1:46894860-46894879 Msa0024480:five_prime_UTR 50.0%
!! ACCACAACCACATTGGCCTT+TGG + chr1_1:46894864-46894883 None:intergenic 50.0%
TGCTTAGAGGCTCCAGTACT+TGG + chr1_1:46894905-46894924 None:intergenic 50.0%
! AGTACTGGAGCCTCTAAGCA+AGG - chr1_1:46894905-46894924 Msa0024480:five_prime_UTR 50.0%
GGAGCCTCTAAGCAAGGAAA+TGG - chr1_1:46894911-46894930 Msa0024480:five_prime_UTR 50.0%
CATGCGCCTAATGGAACCCA+TGG - chr1_1:46892247-46892266 Msa0024480:three_prime_UTR 55.0%
!! GTTCGTCATTTTGGGTCGCG+TGG + chr1_1:46892625-46892644 None:intergenic 55.0%
! AGCAATGGGTTCCAAGGCGA+TGG - chr1_1:46892742-46892761 Msa0024480:intron 55.0%
!! GGCGGCATCGGTTAACTCAA+CGG + chr1_1:46892810-46892829 None:intergenic 55.0%
! TATTCTAGAGGCGGCGGCAT+CGG + chr1_1:46892822-46892841 None:intergenic 55.0%
!! GTGTGCGAGAGTACCACCAA+AGG + chr1_1:46893695-46893714 None:intergenic 55.0%
ATCTGGACAGGCGAGAGGAA+TGG + chr1_1:46893806-46893825 None:intergenic 55.0%
ACATCCCACCTCCTCTCACA+CGG - chr1_1:46893914-46893933 Msa0024480:intron 55.0%
! TGCGGGTGGAAGATTCGGTT+TGG + chr1_1:46893999-46894018 None:intergenic 55.0%
GAATCTTCCACCCGCAACCA+TGG - chr1_1:46894003-46894022 Msa0024480:intron 55.0%
CCCTAGCGCCATCACCAATT+AGG - chr1_1:46894043-46894062 Msa0024480:intron 55.0%
GGGTTCCAAGGCGATGGCTA+TGG - chr1_1:46892748-46892767 Msa0024480:intron 60.0%
TGCTGCCATAGCCATCGCCT+TGG + chr1_1:46892756-46892775 None:intergenic 60.0%
AGATAAGCACGGCGGTGCGA+CGG - chr1_1:46892780-46892799 Msa0024480:intron 60.0%
AAGCTCGGCGTCAAAGCTCG+CGG - chr1_1:46892878-46892897 Msa0024480:intron 60.0%
TCACGTGGGTGACTGTGCTG+AGG - chr1_1:46893353-46893372 Msa0024480:intron 60.0%
CATCCCACCTCCTCTCACAC+GGG - chr1_1:46893915-46893934 Msa0024480:intron 60.0%
GATCCCGTGTGAGAGGAGGT+GGG + chr1_1:46893921-46893940 None:intergenic 60.0%
TGATCCCGTGTGAGAGGAGG+TGG + chr1_1:46893922-46893941 None:intergenic 60.0%
GTAGGATCCATGGTTGCGGG+TGG + chr1_1:46894013-46894032 None:intergenic 60.0%
CGCTAGGGTGAGGGTGATGT+AGG + chr1_1:46894031-46894050 None:intergenic 60.0%
!! TGGTGATGGCGCTAGGGTGA+GGG + chr1_1:46894040-46894059 None:intergenic 60.0%
!! TTGGTGATGGCGCTAGGGTG+AGG + chr1_1:46894041-46894060 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 46892096 46895243 46892096 ID=Msa0024480;Name=Msa0024480
chr1_1 mRNA 46892096 46895243 46892096 ID=Msa0024480-mRNA-1;Parent=Msa0024480;Name=Msa0024480-mRNA-1;_AED=0.01;_eAED=0.01;_QI=650|1|1|1|0.5|1|3|306|137
chr1_1 exon 46892096 46892507 46892096 ID=Msa0024480-mRNA-1:exon:13383;Parent=Msa0024480-mRNA-1
chr1_1 exon 46894180 46894297 46894180 ID=Msa0024480-mRNA-1:exon:13382;Parent=Msa0024480-mRNA-1
chr1_1 exon 46894404 46895243 46894404 ID=Msa0024480-mRNA-1:exon:13381;Parent=Msa0024480-mRNA-1
chr1_1 five_prime_UTR 46894594 46895243 46894594 ID=Msa0024480-mRNA-1:five_prime_utr;Parent=Msa0024480-mRNA-1
chr1_1 CDS 46894404 46894593 46894404 ID=Msa0024480-mRNA-1:cds;Parent=Msa0024480-mRNA-1
chr1_1 CDS 46894180 46894297 46894180 ID=Msa0024480-mRNA-1:cds;Parent=Msa0024480-mRNA-1
chr1_1 CDS 46892402 46892507 46892402 ID=Msa0024480-mRNA-1:cds;Parent=Msa0024480-mRNA-1
chr1_1 three_prime_UTR 46892096 46892401 46892096 ID=Msa0024480-mRNA-1:three_prime_utr;Parent=Msa0024480-mRNA-1
Gene Sequence

>Msa0024480

ATGGGTTCCAAGGCGATGGCTATGGCAGCAGAGAAGATAAGCACGGCGGTGCGACGGCAAGCCGTTGAGTTAACCGATGCCGCCGCCTCTAGAATACACTTCTTGCTTCAGCAACGGCAAAGACCGTTCTTAAAGCTCGGCGTCAAAGCTCGCGGATGTAATGGTTTATCTTACACGCTAAATTACGCAGATAAAAAAGAGAAATTTGATGAATTGGTTGAAGATAAAGGTGTTAAGATTTTGATTGATCCTAAGGCTCTTATGCATGTTGTTGGAACCAAAATGGATTTCGTTGATGATAAACTAAGATCTGAGTTCGTTTTCATTAATCCAAACTCCAAAGGCCAATGTGGTTGTGGTGAATCTTTCATGACAAAACCAAGTACTGGAGCCTCTAAGCAAGGAAATGGTTGA
Protein sequence

>Msa0024480

MGSKAMAMAAEKISTAVRRQAVELTDAAASRIHFLLQQRQRPFLKLGVKARGCNGLSYTLNYADKKEKFDELVEDKGVKILIDPKALMHVVGTKMDFVDDKLRSEFVFINPNSKGQCGCGESFMTKPSTGASKQGNG*