Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1077320 | XP_039683380.1 | 98.699 | 538 | 7 | 0 | 1 | 538 | 1 | 538 | 0.0 | 1112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1077320 | sp|Q5R8M1|STK38_PONAB | 50.448 | 446 | 215 | 4 | 41 | 486 | 10 | 449 | 7.65e-153 | 448 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1077320 | A0A072U4A3 | 98.699 | 538 | 7 | 0 | 1 | 538 | 1 | 538 | 0.0 | 1112 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0680100 | Msa1077320 | 0.813352 | 2.673196e-51 | -8.615850e-47 |
| Msa1036890 | Msa1077320 | 0.888107 | 7.911036e-73 | -8.615850e-47 |
| Msa1077320 | Msa1079100 | 0.852538 | 4.323763e-61 | -8.615850e-47 |
| Msa1077320 | Msa1116990 | 0.822843 | 1.906517e-53 | -8.615850e-47 |
| Msa1077320 | Msa1124010 | 0.836358 | 9.805372e-57 | -8.615850e-47 |
| Msa1077320 | Msa1164000 | 0.859606 | 3.682733e-63 | -8.615850e-47 |
| Msa1077320 | Msa1173470 | 0.801154 | 1.029307e-48 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1077320 | MtrunA17_Chr7g0261491 | 92.028 | 577 | 7 | 2 | 1 | 538 | 1 | 577 | 0.0 | 1089 |
| Msa1077320 | MtrunA17_Chr1g0183651 | 84.328 | 536 | 83 | 1 | 1 | 536 | 1 | 535 | 0.0 | 926 |
| Msa1077320 | MtrunA17_Chr1g0187581 | 75.908 | 523 | 121 | 4 | 1 | 522 | 1 | 519 | 0.0 | 831 |
| Msa1077320 | MtrunA17_Chr1g0167351 | 73.654 | 520 | 123 | 5 | 1 | 518 | 1 | 508 | 0.0 | 771 |
| Msa1077320 | MtrunA17_Chr1g0167311 | 66.853 | 537 | 160 | 8 | 1 | 535 | 1 | 521 | 0.0 | 730 |
| Msa1077320 | MtrunA17_Chr3g0135951 | 65.539 | 473 | 154 | 1 | 44 | 507 | 18 | 490 | 0.0 | 658 |
| Msa1077320 | MtrunA17_Chr2g0299781 | 65.359 | 459 | 150 | 1 | 39 | 488 | 32 | 490 | 0.0 | 635 |
| Msa1077320 | MtrunA17_Chr1g0153321 | 60.516 | 504 | 184 | 5 | 16 | 515 | 9 | 501 | 0.0 | 619 |
| Msa1077320 | MtrunA17_Chr6g0478601 | 36.993 | 419 | 242 | 8 | 31 | 446 | 29 | 428 | 2.79e-84 | 271 |
| Msa1077320 | MtrunA17_Chr4g0018201 | 42.598 | 331 | 170 | 6 | 110 | 435 | 749 | 1064 | 5.81e-78 | 266 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1077320 | AT2G20470.2 | 79.271 | 521 | 99 | 5 | 1 | 516 | 1 | 517 | 0.0 | 842 |
| Msa1077320 | AT2G20470.1 | 79.271 | 521 | 99 | 5 | 1 | 516 | 1 | 517 | 0.0 | 842 |
| Msa1077320 | AT2G20470.3 | 79.231 | 520 | 99 | 5 | 1 | 515 | 1 | 516 | 0.0 | 840 |
| Msa1077320 | AT1G03920.1 | 77.505 | 529 | 101 | 3 | 1 | 512 | 1 | 528 | 0.0 | 838 |
| Msa1077320 | AT1G03920.3 | 77.505 | 529 | 101 | 3 | 1 | 512 | 1 | 528 | 0.0 | 838 |
| Msa1077320 | AT3G23310.1 | 74.432 | 528 | 131 | 3 | 1 | 525 | 1 | 527 | 0.0 | 813 |
| Msa1077320 | AT4G14350.2 | 78.831 | 496 | 101 | 3 | 40 | 534 | 39 | 531 | 0.0 | 811 |
| Msa1077320 | AT4G14350.1 | 78.831 | 496 | 101 | 3 | 40 | 534 | 39 | 531 | 0.0 | 811 |
| Msa1077320 | AT4G14350.4 | 78.831 | 496 | 101 | 3 | 40 | 534 | 39 | 531 | 0.0 | 811 |
| Msa1077320 | AT4G14350.3 | 78.831 | 496 | 101 | 3 | 40 | 534 | 39 | 531 | 0.0 | 811 |
Find 121 sgRNAs with CRISPR-Local
Find 422 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCGGAGATGCTTCGAAG+AGG | 0.667256 | 7_2:+75952115 | Msa1077320:CDS |
| AAATGGATTCAGCGAGGAGT+TGG | 0.488752 | 7_2:+75950629 | None:intergenic |
| AAATGGTGCGGTTGAAATAA+AGG | 0.363488 | 7_2:+75954410 | Msa1077320:CDS |
| AACAAGAACAGTTGGAAAAC+TGG | 0.344829 | 7_2:+75952926 | Msa1077320:CDS |
| AACCAAAGGTTGGGGTCAAA+TGG | 0.449049 | 7_2:+75954393 | Msa1077320:CDS |
| AACCATCTTGGAAAAGAAGT+TGG | 0.526790 | 7_2:+75951756 | Msa1077320:CDS |
| AACCTTCTGCTTGGTAACAT+TGG | 0.513014 | 7_2:-75950761 | None:intergenic |
| AACTTATTACTTGATAAATA+TGG | 0.236706 | 7_2:+75952769 | Msa1077320:CDS |
| AAGAAGGATGATGGTAATGG+CGG | 0.583438 | 7_2:+75950697 | Msa1077320:CDS |
| AAGCTTACTTATAAGATCCG+TGG | 0.658145 | 7_2:-75954358 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCTTCAAGAGAGAAAAGAA+CGG | + | chr7_2:75950833-75950852 | Msa1077320:CDS | 30.0% | |
| GATGACAAACATGAATGAAA+TGG | - | chr7_2:75950865-75950884 | None:intergenic | 30.0% | |
| AGAATCAATTCCAGATGATT+CGG | - | chr7_2:75950904-75950923 | None:intergenic | 30.0% | |
| GCAAGAGAAGTTATTATGAT+TGG | + | chr7_2:75951374-75951393 | Msa1077320:intron | 30.0% | |
| CAACTATTCTTGGAAAATTC+CGG | - | chr7_2:75951414-75951433 | None:intergenic | 30.0% | |
| CAGTACCAATAAGTTAGTAT+AGG | + | chr7_2:75951496-75951515 | Msa1077320:intron | 30.0% | |
| GGAATTATTATTGTGGATGA+TGG | + | chr7_2:75951543-75951562 | Msa1077320:intron | 30.0% | |
| TGATTGCATCATATGAATTG+TGG | - | chr7_2:75951950-75951969 | None:intergenic | 30.0% | |
| ACTAAATGTTATACCTCTAC+TGG | + | chr7_2:75952015-75952034 | Msa1077320:intron | 30.0% | |
| TGTTTCTCCTACATAAAATC+GGG | - | chr7_2:75952425-75952444 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 75950631 | 75957569 | 75950631 | ID=Msa1077320;Name=Msa1077320 |
| chr7_2 | mRNA | 75950631 | 75957569 | 75950631 | ID=Msa1077320-mRNA-1;Parent=Msa1077320;Name=Msa1077320-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0|1|0.91|1|13|0|538 |
| chr7_2 | exon | 75950631 | 75950854 | 75950631 | ID=Msa1077320-mRNA-1:exon:18179;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75951753 | 75951927 | 75951753 | ID=Msa1077320-mRNA-1:exon:18180;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75952058 | 75952141 | 75952058 | ID=Msa1077320-mRNA-1:exon:18181;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75952247 | 75952488 | 75952247 | ID=Msa1077320-mRNA-1:exon:18182;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75952753 | 75952969 | 75952753 | ID=Msa1077320-mRNA-1:exon:18183;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75953121 | 75953200 | 75953121 | ID=Msa1077320-mRNA-1:exon:18184;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75953731 | 75953815 | 75953731 | ID=Msa1077320-mRNA-1:exon:18185;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75954300 | 75954431 | 75954300 | ID=Msa1077320-mRNA-1:exon:18186;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75954522 | 75954635 | 75954522 | ID=Msa1077320-mRNA-1:exon:18187;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75954735 | 75954785 | 75954735 | ID=Msa1077320-mRNA-1:exon:18188;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75955705 | 75955789 | 75955705 | ID=Msa1077320-mRNA-1:exon:18189;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75957338 | 75957394 | 75957338 | ID=Msa1077320-mRNA-1:exon:18190;Parent=Msa1077320-mRNA-1 |
| chr7_2 | exon | 75957499 | 75957569 | 75957499 | ID=Msa1077320-mRNA-1:exon:18191;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75950631 | 75950854 | 75950631 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75951753 | 75951927 | 75951753 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75952058 | 75952141 | 75952058 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75952247 | 75952488 | 75952247 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75952753 | 75952969 | 75952753 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75953121 | 75953200 | 75953121 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75953731 | 75953815 | 75953731 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75954300 | 75954431 | 75954300 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75954522 | 75954635 | 75954522 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75954735 | 75954785 | 75954735 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75955705 | 75955789 | 75955705 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75957338 | 75957394 | 75957338 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| chr7_2 | CDS | 75957499 | 75957569 | 75957499 | ID=Msa1077320-mRNA-1:cds;Parent=Msa1077320-mRNA-1 |
| Gene Sequence |
>Msa1077320 ATGGATTCAGCGAGGAGTTGGTTTCATAAGTTTCAACCGCGAGATAGACTGAGAGCTTCAACGAGGAAGAAGGATGATGGTAATGGCGGAATAGATGATGCCAATGCGGTTGTTGATGAGACGGATCTTTCCAATGTTACCAAGCAGAAGGTTGCTGCTGCTAAGCAATATATCGAGAATCATTATAAAGAACAAATGAAGAATCTTCAAGAGAGAAAAGAACGTCGAACCATCTTGGAAAAGAAGTTGGCTGATGCTGATGTCTCTGAGGAAGATCAAAATAACCTACTTAAATATTTAGCGAAGAAGGAAACCGAATATATGCGTCTTCAAAGGCATAAAATGGGTGTTGAGGATTTTGAGATGTTGACTATGATTGGAAAAGGTGCATTTGGGGAGGTTAGAGTCTGCAGGGAAAAGACTACAGATCATGTATATGCAATGAAAAAGCTAAAGAAATCGGAGATGCTTCGAAGAGGCCAGGTAGAACATGTCAGAGCTGAGAGGAATCTCCTTGCAGAGGTTGACAGCAATTGCATAGTCAAACTTTATTGTTCTTTCCAAGATGATGAGTATTTATATCTTATTATGGAGTATCTACCGGGAGGGGATATGATGACTCTTCTTATGAGAAAGGATACATTAACCGAAGACGAAGCCCGATTTTATGTAGGAGAAACAGTTTTGGCTATTGAATCTATACATAAACACAATTATATACATAGGGATATTAAACCTGATAACTTATTACTTGATAAATATGGACACTTGAAACTATCGGATTTTGGACTATGTAAACCGTTAGACTGTAGTGTACTTGAAGAATCAGACTTTTCTAATTGCCAGAATGCAAATGGAACTGCACAAAATGATGATCGTGCATTTCCGAAACGTACACAACAAGAACAGTTGGAAAACTGGCAGAAAAACAGGAGGACACTTGCTTATTCTACCGTTGGTACACCAGATTATATTGCTCCAGAAGTTTTATTGAAGAAAGGTTATGGGATGGAATGTGATTGGTGGTCACTTGGAGCTATTATGTATGAAATGTTGGTGGGATATCCACCATTTTATTCTGATGATCCAATGTCAACATGTAGGAAGATAGTAAACTGGAAGTCTCACTTGAAGTTTCCCGAAGAAGCAAGGCTATCTCCGGAGGCCACGGATCTTATAAGTAAGCTTTTGTGTAATGTCAACCAAAGGTTGGGGTCAAATGGTGCGGTTGAAATAAAGTCTCATCCATTCTTTGAAGGTGTTCAATGGGAAAAGTTGTATCAAATGGAAGCTGCATTTACTCCTGAAGTCAACGACGAGTTAGATACTCAAAATTTTGAAAAGTTTGAAGAGTCTGACAGCCAGACCCATTCATCATCAAGATCAGGTGGTCCATGGAGAAAGATGCTTTCATCGAAGGACATAAATTTTGTTGGATATACATACAAGAACTTTGAGATTGTTAACGACTATCAAGTTCCTGGGATGGCTGAGTTGAAGAAAACCTCCAAATCGAAGAGGAAGCCATCTGTCAAGTCGTTGTTTGATGGCGAGTTGGAGACTTCTGAGGTGTCTGATACTTCAGAAGTGTCTGCAAGCAATCCACCTACTAAATGA |
| Protein sequence |
>Msa1077320 MDSARSWFHKFQPRDRLRASTRKKDDGNGGIDDANAVVDETDLSNVTKQKVAAAKQYIENHYKEQMKNLQERKERRTILEKKLADADVSEEDQNNLLKYLAKKETEYMRLQRHKMGVEDFEMLTMIGKGAFGEVRVCREKTTDHVYAMKKLKKSEMLRRGQVEHVRAERNLLAEVDSNCIVKLYCSFQDDEYLYLIMEYLPGGDMMTLLMRKDTLTEDEARFYVGETVLAIESIHKHNYIHRDIKPDNLLLDKYGHLKLSDFGLCKPLDCSVLEESDFSNCQNANGTAQNDDRAFPKRTQQEQLENWQKNRRTLAYSTVGTPDYIAPEVLLKKGYGMECDWWSLGAIMYEMLVGYPPFYSDDPMSTCRKIVNWKSHLKFPEEARLSPEATDLISKLLCNVNQRLGSNGAVEIKSHPFFEGVQWEKLYQMEAAFTPEVNDELDTQNFEKFEESDSQTHSSSRSGGPWRKMLSSKDINFVGYTYKNFEIVNDYQVPGMAELKKTSKSKRKPSVKSLFDGELETSEVSDTSEVSASNPPTK* |
