AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1173470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 85 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGAAAAGATGAACAATTT+TGG 0.161614 8_1:-3862320 None:intergenic
AAGCATCACAATATTGAATT+TGG 0.165572 8_1:-3864904 None:intergenic
CGATTATATATTCTAGAAAT+AGG 0.252654 8_1:-3865061 None:intergenic
AATATTGTATATTTCCAAAA+TGG 0.253982 8_1:-3865202 None:intergenic
GTAGCACCTTCAACGATAAA+TGG 0.301094 8_1:+3861948 Msa1173470:five_prime_UTR
TCATCCCCTTAGAACCCTTA+TGG 0.306841 8_1:+3862199 Msa1173470:five_prime_UTR
TTGACTCCATAAGGGTTCTA+AGG 0.323844 8_1:-3862205 None:intergenic
ATAAAATGTTGATGTTCATT+AGG 0.330627 8_1:-3865143 None:intergenic
GCAGAGTTCATTGGCATACT+AGG 0.350554 8_1:+3863459 Msa1173470:intron
AAAGTGGAGCGTGAGTTTAT+TGG 0.354845 8_1:-3861816 None:intergenic
CGGTTTCCATTTATCGTTGA+AGG 0.365309 8_1:-3861954 None:intergenic
TAGATCTAGAAATAGGTTAC+TGG 0.381185 8_1:-3865025 None:intergenic
TGAAATCAAGAATGGGAGTT+TGG 0.382622 8_1:-3865106 None:intergenic
AAAGAATGATTGACTCCATA+AGG 0.383943 8_1:-3862214 None:intergenic
GGTTATGTAGATCTAGAAAT+AGG 0.392162 8_1:-3865032 None:intergenic
AGGACGATTGATAGGCAAAT+AGG 0.394105 8_1:-3863192 None:intergenic
TCTTAGCTGCAAGACACTTA+AGG 0.399730 8_1:-3863212 None:intergenic
AATCGGAGGTGTTGAATGTT+CGG 0.406634 8_1:+3862291 Msa1173470:exon
TTAATCTTTGCAGAGTTCAT+TGG 0.415750 8_1:+3863450 Msa1173470:intron
TCTCCCACCACTACCGTCTC+TGG 0.419830 8_1:+3861846 Msa1173470:five_prime_UTR
ATCGGAGGTGTTGAATGTTC+GGG 0.420607 8_1:+3862292 Msa1173470:exon
TACATGTCAAATATTTGAAT+GGG 0.424772 8_1:-3863279 None:intergenic
ACAAATAAAACCTTCGGATA+AGG 0.434235 8_1:-3863484 None:intergenic
ACGCAATTCAATTCATAGAA+AGG 0.440026 8_1:-3862146 None:intergenic
CTATTCAATTTCGTCGATTG+AGG 0.440240 8_1:+3862368 Msa1173470:CDS
CGCAATTCAATTCATAGAAA+GGG 0.450972 8_1:-3862145 None:intergenic
GACACTTAAGGACGATTGAT+AGG 0.454087 8_1:-3863200 None:intergenic
ATACATGTCAAATATTTGAA+TGG 0.457197 8_1:-3863280 None:intergenic
TATTCTAGAAATAGGTTATG+TGG 0.470056 8_1:-3865053 None:intergenic
TCTTCTGAACAAGGCGGAGG+TGG 0.475561 8_1:+3862032 Msa1173470:five_prime_UTR
ATACAGCATGAAATCAAGAA+TGG 0.482965 8_1:-3865114 None:intergenic
ATCCTCCACGCCGTCAGCTG+TGG 0.491291 8_1:+3861900 Msa1173470:five_prime_UTR
AGTCATCGAAACGGAGAAGA+AGG 0.493305 8_1:-3862347 None:intergenic
GGTTTGATTCGCATGCCATT+CGG 0.494201 8_1:-3861974 None:intergenic
CTCCGCCTTGTTCAGAAGAT+CGG 0.504922 8_1:-3862028 None:intergenic
TGACTCCATAAGGGTTCTAA+GGG 0.506680 8_1:-3862204 None:intergenic
ATTTCAAGTCACAAGTGCAT+TGG 0.508716 8_1:+3861772 None:intergenic
ATTCTAGATCTAAAAGAAAG+TGG 0.520543 8_1:-3864998 None:intergenic
TACAGCATGAAATCAAGAAT+GGG 0.520608 8_1:-3865113 None:intergenic
AGTTCCAGAGACGGTAGTGG+TGG 0.523926 8_1:-3861850 None:intergenic
TAAGCTAAGGCCGTCGATGA+TGG 0.526649 8_1:+3863165 Msa1173470:intron
TCCCTCCTTCTTCTTCACAT+CGG 0.528047 8_1:-3862064 None:intergenic
AGAAGCCGATGTGAAGAAGA+AGG 0.532467 8_1:+3862059 Msa1173470:five_prime_UTR
GAGATGACGGAGGGAGGCAA+TGG 0.532740 8_1:-3861877 None:intergenic
AAGAATGATTGACTCCATAA+GGG 0.538167 8_1:-3862213 None:intergenic
GGTGGGAGAATATTGAAAAG+TGG 0.540273 8_1:-3861832 None:intergenic
CGATCTTCTGAACAAGGCGG+AGG 0.548594 8_1:+3862029 Msa1173470:five_prime_UTR
AATATTTGAATGGGGTGCAA+AGG 0.551607 8_1:-3863270 None:intergenic
GGGACTGAAGCGGCATGTGA+AGG 0.554999 8_1:+3862083 Msa1173470:five_prime_UTR
GTTGACTGATAACAATCCAT+TGG 0.562982 8_1:+3862673 Msa1173470:CDS
GAGAGTTCCAGAGACGGTAG+TGG 0.567901 8_1:-3861853 None:intergenic
ATACTAGGTTCCTTATCCGA+AGG 0.568215 8_1:+3863474 Msa1173470:CDS
GCCGATGTGAAGAAGAAGGA+GGG 0.579212 8_1:+3862063 Msa1173470:five_prime_UTR
GCTTTCCGATCTTCTGAACA+AGG 0.584830 8_1:+3862023 Msa1173470:five_prime_UTR
AAGAGGAAGCTGACTATGTG+GGG 0.591978 8_1:+3862639 Msa1173470:CDS
AACGGAAAAGCCACAGCTGA+CGG 0.595636 8_1:-3861910 None:intergenic
AAATTGAATAGTCATCGAAA+CGG 0.596702 8_1:-3862356 None:intergenic
AGTCGTCTCAAGCGTTAGAG+AGG 0.597652 8_1:-3862256 None:intergenic
CTCAAAATTGAATGTGACGT+CGG 0.609907 8_1:+3861996 Msa1173470:five_prime_UTR
GTTCCAGAGACGGTAGTGGT+GGG 0.611665 8_1:-3861849 None:intergenic
AACGATAAATGGAAACCGAA+TGG 0.612701 8_1:+3861959 Msa1173470:five_prime_UTR
GAAGAGGAAGCTGACTATGT+GGG 0.616858 8_1:+3862638 Msa1173470:CDS
CGTTGAAGGTGCTACAACGG+TGG 0.622090 8_1:-3861940 None:intergenic
CGGCGTGGAGGATGAGATGA+CGG 0.626771 8_1:-3861890 None:intergenic
GAGGATGAGATGACGGAGGG+AGG 0.629445 8_1:-3861883 None:intergenic
TATCGTTGAAGGTGCTACAA+CGG 0.633875 8_1:-3861943 None:intergenic
AATAGGAACACCATCATCGA+CGG 0.635123 8_1:-3863175 None:intergenic
TCAGCTCGGATATGATGAAG+AGG 0.636784 8_1:+3862622 Msa1173470:intron
AAAAGCCACAGCTGACGGCG+TGG 0.639850 8_1:-3861905 None:intergenic
AGCCGATGTGAAGAAGAAGG+AGG 0.642473 8_1:+3862062 Msa1173470:five_prime_UTR
GTCGTCTCAAGCGTTAGAGA+GGG 0.643738 8_1:-3862255 None:intergenic
AGAAGAAGGAGGGACTGAAG+CGG 0.644736 8_1:+3862073 Msa1173470:five_prime_UTR
GTGGAGGATGAGATGACGGA+GGG 0.645414 8_1:-3861886 None:intergenic
AGCCACAGCTGACGGCGTGG+AGG 0.648146 8_1:-3861902 None:intergenic
TGAAGAGGAAGCTGACTATG+TGG 0.652797 8_1:+3862637 Msa1173470:CDS
TACAACGGTGGTGATTGTAA+CGG 0.659540 8_1:-3861928 None:intergenic
AATGGAGAGAGTTCCAGAGA+CGG 0.663560 8_1:-3861859 None:intergenic
TAAATGTGAACGGAGACCGA+CGG 0.671648 8_1:-3863238 None:intergenic
ACATGTCAAATATTTGAATG+GGG 0.675084 8_1:-3863278 None:intergenic
CTTGCAGCTAAGATAACCGT+CGG 0.680082 8_1:+3863222 Msa1173470:CDS
GAATTGCAGATAAATGTGAA+CGG 0.680664 8_1:-3863248 None:intergenic
TTCCGATCTTCTGAACAAGG+CGG 0.700783 8_1:+3862026 Msa1173470:five_prime_UTR
CGTGGAGGATGAGATGACGG+AGG 0.703543 8_1:-3861887 None:intergenic
GACTCCATAAGGGTTCTAAG+GGG 0.711536 8_1:-3862203 None:intergenic
AGAGGAAGCTGACTATGTGG+GGG 0.774034 8_1:+3862640 Msa1173470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAATTAGTATTGTAA+TGG + chr8_1:3863792-3863811 Msa1173470:intron 10.0%
!! ATAAAAATTAGTATTGTAAT+GGG + chr8_1:3863793-3863812 Msa1173470:intron 10.0%
!! AAGAATGACTTATATTAAAA+TGG - chr8_1:3863030-3863049 None:intergenic 15.0%
!!! ATTTTAATATAAGTCATTCT+TGG + chr8_1:3863029-3863048 Msa1173470:intron 15.0%
!! TAAAAAAATTGCTTACAATT+AGG + chr8_1:3863962-3863981 Msa1173470:intron 15.0%
!! ATTCAAAATACTACAAAATA+TGG - chr8_1:3864348-3864367 None:intergenic 15.0%
!!! ATATTTTGTAGTATTTTGAA+TGG + chr8_1:3864347-3864366 Msa1173470:intron 15.0%
!!! TATTTTGTAGTATTTTGAAT+GGG + chr8_1:3864348-3864367 Msa1173470:intron 15.0%
!!! TCATTATAATCCATATTTTT+AGG + chr8_1:3864458-3864477 Msa1173470:intron 15.0%
!! TAAAAAGAAAAAAACATTGT+CGG - chr8_1:3864547-3864566 None:intergenic 15.0%
!!! TCCAATTAATTTTGTATCAA+TGG + chr8_1:3862836-3862855 Msa1173470:intron 20.0%
!! TCCATTGATACAAAATTAAT+TGG - chr8_1:3862840-3862859 None:intergenic 20.0%
!! AGAACTCTTAAAATAAGAAA+TGG - chr8_1:3862945-3862964 None:intergenic 20.0%
!! TACATGTCAAATATTTGAAT+GGG - chr8_1:3863282-3863301 None:intergenic 20.0%
!! ATACATGTCAAATATTTGAA+TGG - chr8_1:3863283-3863302 None:intergenic 20.0%
!! GAAGATAAATAATCAAAGAA+TGG - chr8_1:3863311-3863330 None:intergenic 20.0%
!! TTTATTTGTCCATTTGATTA+TGG + chr8_1:3863498-3863517 Msa1173470:intron 20.0%
!! TTTATTTGAATGAATGACAT+TGG + chr8_1:3864221-3864240 Msa1173470:intron 20.0%
!!! GACAAAATTCTATATTTTGT+TGG + chr8_1:3864294-3864313 Msa1173470:intron 20.0%
!!! ATTTTGTAGTATTTTGAATG+GGG + chr8_1:3864349-3864368 Msa1173470:intron 20.0%
!!! ATATAGCATAATGTGTATTT+TGG + chr8_1:3864372-3864391 Msa1173470:intron 20.0%
!!! AATATAAGCATATACCTTTT+GGG - chr8_1:3864485-3864504 None:intergenic 20.0%
!! AAATTCAATCAGATTCTTTA+AGG - chr8_1:3864777-3864796 None:intergenic 20.0%
!!! TTTTCATTATTTCTTTTTGC+AGG + chr8_1:3864862-3864881 Msa1173470:intron 20.0%
!! AAAAAGATGTTCACAAAAAA+AGG + chr8_1:3864952-3864971 Msa1173470:CDS 20.0%
!!! CGATTATATATTCTAGAAAT+AGG - chr8_1:3865064-3865083 None:intergenic 20.0%
!! ATAAAATGTTGATGTTCATT+AGG - chr8_1:3865146-3865165 None:intergenic 20.0%
!!! TTTTCAATAGATATCCATTT+TGG + chr8_1:3865188-3865207 Msa1173470:three_prime_UTR 20.0%
!! GTGAAAAGATGAACAATTTT+GGG - chr8_1:3862322-3862341 None:intergenic 25.0%
!! AGTGAAAAGATGAACAATTT+TGG - chr8_1:3862323-3862342 None:intergenic 25.0%
! AAATTGAATAGTCATCGAAA+CGG - chr8_1:3862359-3862378 None:intergenic 25.0%
! GAATTCAAAATTAAATAGCC+AGG - chr8_1:3862434-3862453 None:intergenic 25.0%
! TCACTTTCTTAATGCAATTA+AGG - chr8_1:3862456-3862475 None:intergenic 25.0%
! AAACAGAAACACAGTAATTT+AGG - chr8_1:3862599-3862618 None:intergenic 25.0%
!! TAAAAGCATGTTTATACCAA+TGG - chr8_1:3862692-3862711 None:intergenic 25.0%
! ATAATAAACATTGCAAGCTT+AGG + chr8_1:3862919-3862938 Msa1173470:intron 25.0%
! TTAATATAAGTCATTCTTGG+TGG + chr8_1:3863032-3863051 Msa1173470:intron 25.0%
! GTTTCAAAACAAGTAAGTTT+AGG + chr8_1:3863071-3863090 Msa1173470:intron 25.0%
! ACATGTCAAATATTTGAATG+GGG - chr8_1:3863281-3863300 None:intergenic 25.0%
! AAATGGACAAATAAAACCTT+CGG - chr8_1:3863493-3863512 None:intergenic 25.0%
!!! CATTTTCCTGTTTTTGTTTT+TGG + chr8_1:3863541-3863560 Msa1173470:intron 25.0%
! TTTCAGAAGTTATCAAATTG+TGG - chr8_1:3863697-3863716 None:intergenic 25.0%
! GATAACTATGCATACTATTA+TGG + chr8_1:3863729-3863748 Msa1173470:intron 25.0%
!!! GCAATTTTTTTATCTCATGT+AGG - chr8_1:3863954-3863973 None:intergenic 25.0%
!!! TCTGAAATGTGAAAATGTTT+TGG + chr8_1:3864129-3864148 Msa1173470:intron 25.0%
!!! TAATGTGTATTTTGGTTCAT+CGG + chr8_1:3864380-3864399 Msa1173470:intron 25.0%
!! ATATAAGCATATACCTTTTG+GGG - chr8_1:3864484-3864503 None:intergenic 25.0%
!! CAATATAAGCATATACCTTT+TGG - chr8_1:3864486-3864505 None:intergenic 25.0%
! TTTCTTTCCATTACAATAAC+TGG - chr8_1:3864588-3864607 None:intergenic 25.0%
!! TTATTGTAATGGAAAGAAAC+AGG + chr8_1:3864589-3864608 Msa1173470:intron 25.0%
! ATTTGTGGTGAAAACAAATA+GGG + chr8_1:3864621-3864640 Msa1173470:intron 25.0%
!!! TTGTGACTTTAAAATGATAC+AGG + chr8_1:3864691-3864710 Msa1173470:intron 25.0%
!! TTTAAAATGATACAGGTTAG+TGG + chr8_1:3864698-3864717 Msa1173470:intron 25.0%
! TTAAAATGATACAGGTTAGT+GGG + chr8_1:3864699-3864718 Msa1173470:intron 25.0%
! AAGCATCACAATATTGAATT+TGG - chr8_1:3864907-3864926 None:intergenic 25.0%
! ATTCTAGATCTAAAAGAAAG+TGG - chr8_1:3865001-3865020 None:intergenic 25.0%
!! TATTCTAGAAATAGGTTATG+TGG - chr8_1:3865056-3865075 None:intergenic 25.0%
! GTTTTGTGAATTTTCTCTCA+AGG + chr8_1:3862111-3862130 Msa1173470:five_prime_UTR 30.0%
CGCAATTCAATTCATAGAAA+GGG - chr8_1:3862148-3862167 None:intergenic 30.0%
ACGCAATTCAATTCATAGAA+AGG - chr8_1:3862149-3862168 None:intergenic 30.0%
! AAGAATGATTGACTCCATAA+GGG - chr8_1:3862216-3862235 None:intergenic 30.0%
! AAAGAATGATTGACTCCATA+AGG - chr8_1:3862217-3862236 None:intergenic 30.0%
! CGACTCAGATTTTCAAAAAT+CGG + chr8_1:3862274-3862293 Msa1173470:five_prime_UTR 30.0%
TTCCATGTTGATATATGAAG+CGG - chr8_1:3862501-3862520 None:intergenic 30.0%
TGTTGAATCTGTGTTAGTAA+TGG + chr8_1:3862716-3862735 Msa1173470:intron 30.0%
CTTGAATCAATTATTGATGC+AGG + chr8_1:3862743-3862762 Msa1173470:intron 30.0%
TTCTTTATTGCCTTGCTTTA+TGG - chr8_1:3862869-3862888 None:intergenic 30.0%
GCTTGCAATGTTTATTATGA+TGG - chr8_1:3862917-3862936 None:intergenic 30.0%
AAAATAAAAAAAGGAGCAGC+AGG - chr8_1:3862999-3863018 None:intergenic 30.0%
TACTACTAACAGAGAATTGA+AGG - chr8_1:3863122-3863141 None:intergenic 30.0%
GAATTGCAGATAAATGTGAA+CGG - chr8_1:3863251-3863270 None:intergenic 30.0%
TTAATCTTTGCAGAGTTCAT+TGG + chr8_1:3863450-3863469 Msa1173470:intron 30.0%
ACAAATAAAACCTTCGGATA+AGG - chr8_1:3863487-3863506 None:intergenic 30.0%
ATGCATCCAAAAACAAAAAC+AGG - chr8_1:3863550-3863569 None:intergenic 30.0%
TATCAAATTGTGGTTACCTT+TGG - chr8_1:3863687-3863706 None:intergenic 30.0%
! CTCATGTAGGATTTTATCTT+TGG - chr8_1:3863941-3863960 None:intergenic 30.0%
AACCTGATTTCATATCCTTT+GGG - chr8_1:3863991-3864010 None:intergenic 30.0%
! GAAATCAGGTTGTCAAAAAA+TGG + chr8_1:3864000-3864019 Msa1173470:intron 30.0%
! TGGCTATACTTTTATACCAA+AGG + chr8_1:3864020-3864039 Msa1173470:intron 30.0%
ATACCAAAGGATATGAAATC+AGG + chr8_1:3864033-3864052 Msa1173470:intron 30.0%
!! GAAATGTGAAAATGTTTTGG+CGG + chr8_1:3864132-3864151 Msa1173470:intron 30.0%
TGAACTATTGGGAAATCATA+TGG + chr8_1:3864174-3864193 Msa1173470:intron 30.0%
!! TATTTTGTTGGCTGAACTAT+TGG + chr8_1:3864306-3864325 Msa1173470:intron 30.0%
!! ATTTTGTTGGCTGAACTATT+GGG + chr8_1:3864307-3864326 Msa1173470:intron 30.0%
ACATTCAAGGAGAAAAATTG+CGG - chr8_1:3864427-3864446 None:intergenic 30.0%
CATTTGTGGTGAAAACAAAT+AGG + chr8_1:3864620-3864639 Msa1173470:intron 30.0%
!! ATTCAACTGAGAGTATTCAT+AGG + chr8_1:3864664-3864683 Msa1173470:intron 30.0%
!! TTCAACTGAGAGTATTCATA+GGG + chr8_1:3864665-3864684 Msa1173470:intron 30.0%
TAAAATGATACAGGTTAGTG+GGG + chr8_1:3864700-3864719 Msa1173470:intron 30.0%
! GGGGATTAAAATAGTCTTTT+TGG + chr8_1:3864720-3864739 Msa1173470:intron 30.0%
! ATTTCTTTTTGCAGGTTATG+TGG + chr8_1:3864870-3864889 Msa1173470:intron 30.0%
TAGATCTAGAAATAGGTTAC+TGG - chr8_1:3865028-3865047 None:intergenic 30.0%
GGTTATGTAGATCTAGAAAT+AGG - chr8_1:3865035-3865054 None:intergenic 30.0%
TACAGCATGAAATCAAGAAT+GGG - chr8_1:3865116-3865135 None:intergenic 30.0%
ATACAGCATGAAATCAAGAA+TGG - chr8_1:3865117-3865136 None:intergenic 30.0%
AACGATAAATGGAAACCGAA+TGG + chr8_1:3861959-3861978 Msa1173470:five_prime_UTR 35.0%
CTCAAAATTGAATGTGACGT+CGG + chr8_1:3861996-3862015 Msa1173470:five_prime_UTR 35.0%
! CTCAGATTTTCAAAAATCGG+AGG + chr8_1:3862277-3862296 Msa1173470:five_prime_UTR 35.0%
! CTATTCAATTTCGTCGATTG+AGG + chr8_1:3862368-3862387 Msa1173470:CDS 35.0%
AACCGCTTCATATATCAACA+TGG + chr8_1:3862496-3862515 Msa1173470:intron 35.0%
! TGTTTCTGTTTATCTCAGCT+CGG + chr8_1:3862608-3862627 Msa1173470:intron 35.0%
GTTGACTGATAACAATCCAT+TGG + chr8_1:3862673-3862692 Msa1173470:CDS 35.0%
TGGAGAATTTCCATAAAGCA+AGG + chr8_1:3862856-3862875 Msa1173470:intron 35.0%
AGTAAGTTTAGGCCAACTAT+AGG + chr8_1:3863082-3863101 Msa1173470:intron 35.0%
AACCTTTCTGATCCTATAGT+TGG - chr8_1:3863097-3863116 None:intergenic 35.0%
AACTATAGGATCAGAAAGGT+TGG + chr8_1:3863096-3863115 Msa1173470:intron 35.0%
TCTGTTAGTAGTATCTCGAT+CGG + chr8_1:3863129-3863148 Msa1173470:intron 35.0%
TAAGGCCAACAAATAAGCTA+AGG + chr8_1:3863152-3863171 Msa1173470:intron 35.0%
AATATTTGAATGGGGTGCAA+AGG - chr8_1:3863273-3863292 None:intergenic 35.0%
CTCTGCAAAGATTAACGAAA+AGG - chr8_1:3863445-3863464 None:intergenic 35.0%
CTAGCTTGACCATAATCAAA+TGG - chr8_1:3863510-3863529 None:intergenic 35.0%
CATTTGATTATGGTCAAGCT+AGG + chr8_1:3863508-3863527 Msa1173470:intron 35.0%
TATGGTCAAGCTAGGTAAAT+TGG + chr8_1:3863516-3863535 Msa1173470:intron 35.0%
! ATTGAGCATCGCTGTATATA+GGG + chr8_1:3863575-3863594 Msa1173470:intron 35.0%
TCCTACTGAATAATGACCAA+AGG + chr8_1:3863668-3863687 Msa1173470:intron 35.0%
ACCTTTGGTCATTATTCAGT+AGG - chr8_1:3863672-3863691 None:intergenic 35.0%
! AGTATTGTAATGGGAATGTG+TGG + chr8_1:3863802-3863821 Msa1173470:intron 35.0%
ATCAGGATTGTAGGAATCTA+TGG + chr8_1:3863832-3863851 Msa1173470:intron 35.0%
! CTCTTGGATGGATTCATATA+TGG - chr8_1:3863908-3863927 None:intergenic 35.0%
CATATATGAATCCATCCAAG+AGG + chr8_1:3863906-3863925 Msa1173470:intron 35.0%
CTTACAATTAGGTTTCCCAA+AGG + chr8_1:3863973-3863992 Msa1173470:intron 35.0%
TTCCCAAAGGATATGAAATC+AGG + chr8_1:3863986-3864005 Msa1173470:intron 35.0%
CAACCTGATTTCATATCCTT+TGG - chr8_1:3863992-3864011 None:intergenic 35.0%
GAAATCAGGTCGTCAAAAAA+TGG + chr8_1:3864047-3864066 Msa1173470:intron 35.0%
AAGCCCTATAAAATACCATC+AGG + chr8_1:3864090-3864109 Msa1173470:intron 35.0%
! GAGCCTGATGGTATTTTATA+GGG - chr8_1:3864096-3864115 None:intergenic 35.0%
! TGAGCCTGATGGTATTTTAT+AGG - chr8_1:3864097-3864116 None:intergenic 35.0%
TTTGGCGGTAATTTGAGAAT+TGG + chr8_1:3864147-3864166 Msa1173470:intron 35.0%
!! ATGAATGACATTGGTGTCAT+TGG + chr8_1:3864230-3864249 Msa1173470:intron 35.0%
CAACGTTCCAGTTATTGTAA+TGG + chr8_1:3864578-3864597 Msa1173470:intron 35.0%
AAAATGATACAGGTTAGTGG+GGG + chr8_1:3864701-3864720 Msa1173470:intron 35.0%
AAGGCAATTGCTTCTTTCAT+TGG - chr8_1:3864758-3864777 None:intergenic 35.0%
TGAAATCAAGAATGGGAGTT+TGG - chr8_1:3865109-3865128 None:intergenic 35.0%
AAAGTGGAGCGTGAGTTTAT+TGG - chr8_1:3861819-3861838 None:intergenic 40.0%
GGTGGGAGAATATTGAAAAG+TGG - chr8_1:3861835-3861854 None:intergenic 40.0%
TACAACGGTGGTGATTGTAA+CGG - chr8_1:3861931-3861950 None:intergenic 40.0%
!! TATCGTTGAAGGTGCTACAA+CGG - chr8_1:3861946-3861965 None:intergenic 40.0%
GTAGCACCTTCAACGATAAA+TGG + chr8_1:3861948-3861967 Msa1173470:five_prime_UTR 40.0%
CGGTTTCCATTTATCGTTGA+AGG - chr8_1:3861957-3861976 None:intergenic 40.0%
! CGACGTCACATTCAATTTTG+AGG - chr8_1:3861998-3862017 None:intergenic 40.0%
! TGACTCCATAAGGGTTCTAA+GGG - chr8_1:3862207-3862226 None:intergenic 40.0%
TTGACTCCATAAGGGTTCTA+AGG - chr8_1:3862208-3862227 None:intergenic 40.0%
!! AATCGGAGGTGTTGAATGTT+CGG + chr8_1:3862291-3862310 Msa1173470:exon 40.0%
! TTCGTCGATTGAGGTAAGAA+TGG + chr8_1:3862377-3862396 Msa1173470:intron 40.0%
CGATTGAGGTAAGAATGGAA+TGG + chr8_1:3862382-3862401 Msa1173470:intron 40.0%
GAGGTAAGAATGGAATGGAA+TGG + chr8_1:3862387-3862406 Msa1173470:intron 40.0%
GTGTTACATCAGTTTAGACC+TGG + chr8_1:3862413-3862432 Msa1173470:intron 40.0%
!! ACAGTTCTTAAACTTGCTGC+AGG - chr8_1:3862972-3862991 None:intergenic 40.0%
TAGTAGTATCTCGATCGGTA+AGG + chr8_1:3863134-3863153 Msa1173470:intron 40.0%
AATAGGAACACCATCATCGA+CGG - chr8_1:3863178-3863197 None:intergenic 40.0%
AGGACGATTGATAGGCAAAT+AGG - chr8_1:3863195-3863214 None:intergenic 40.0%
GACACTTAAGGACGATTGAT+AGG - chr8_1:3863203-3863222 None:intergenic 40.0%
TCTTAGCTGCAAGACACTTA+AGG - chr8_1:3863215-3863234 None:intergenic 40.0%
ATACTAGGTTCCTTATCCGA+AGG + chr8_1:3863474-3863493 Msa1173470:CDS 40.0%
! CATTGAGCATCGCTGTATAT+AGG + chr8_1:3863574-3863593 Msa1173470:intron 40.0%
TTGTAGGAATCTATGGCACA+TGG + chr8_1:3863839-3863858 Msa1173470:intron 40.0%
TATGAATCCATCCAAGAGGA+AGG + chr8_1:3863910-3863929 Msa1173470:intron 40.0%
CGACCTGATTTCATATCCTT+TGG - chr8_1:3864039-3864058 None:intergenic 40.0%
! AGAATTGGTGGCTGAACTAT+TGG + chr8_1:3864162-3864181 Msa1173470:intron 40.0%
! GAATTGGTGGCTGAACTATT+GGG + chr8_1:3864163-3864182 Msa1173470:intron 40.0%
GAACTCGCATAACACATTCA+AGG - chr8_1:3864440-3864459 None:intergenic 40.0%
!!! CCTTTTGGGGCCTAAAAATA+TGG - chr8_1:3864471-3864490 None:intergenic 40.0%
!!! CCATATTTTTAGGCCCCAAA+AGG + chr8_1:3864468-3864487 Msa1173470:intron 40.0%
AACAGGATCTGCTACATTTG+TGG + chr8_1:3864606-3864625 Msa1173470:intron 40.0%
AATGGAGAGAGTTCCAGAGA+CGG - chr8_1:3861862-3861881 None:intergenic 45.0%
! GGTTTGATTCGCATGCCATT+CGG - chr8_1:3861977-3861996 None:intergenic 45.0%
GCTTTCCGATCTTCTGAACA+AGG + chr8_1:3862023-3862042 Msa1173470:five_prime_UTR 45.0%
TTCCGATCTTCTGAACAAGG+CGG + chr8_1:3862026-3862045 Msa1173470:five_prime_UTR 45.0%
AGAAGCCGATGTGAAGAAGA+AGG + chr8_1:3862059-3862078 Msa1173470:five_prime_UTR 45.0%
TCCCTCCTTCTTCTTCACAT+CGG - chr8_1:3862067-3862086 None:intergenic 45.0%
TCATCCCCTTAGAACCCTTA+TGG + chr8_1:3862199-3862218 Msa1173470:five_prime_UTR 45.0%
!! GACTCCATAAGGGTTCTAAG+GGG - chr8_1:3862206-3862225 None:intergenic 45.0%
!! ATCGGAGGTGTTGAATGTTC+GGG + chr8_1:3862292-3862311 Msa1173470:exon 45.0%
AGTCATCGAAACGGAGAAGA+AGG - chr8_1:3862350-3862369 None:intergenic 45.0%
TCAGCTCGGATATGATGAAG+AGG + chr8_1:3862622-3862641 Msa1173470:intron 45.0%
TGAAGAGGAAGCTGACTATG+TGG + chr8_1:3862637-3862656 Msa1173470:CDS 45.0%
GAAGAGGAAGCTGACTATGT+GGG + chr8_1:3862638-3862657 Msa1173470:CDS 45.0%
AAGAGGAAGCTGACTATGTG+GGG + chr8_1:3862639-3862658 Msa1173470:CDS 45.0%
GGCCAACTATAGGATCAGAA+AGG + chr8_1:3863092-3863111 Msa1173470:intron 45.0%
GACGGCCTTAGCTTATTTGT+TGG - chr8_1:3863160-3863179 None:intergenic 45.0%
CTTGCAGCTAAGATAACCGT+CGG + chr8_1:3863222-3863241 Msa1173470:CDS 45.0%
TAAATGTGAACGGAGACCGA+CGG - chr8_1:3863241-3863260 None:intergenic 45.0%
GCAGAGTTCATTGGCATACT+AGG + chr8_1:3863459-3863478 Msa1173470:intron 45.0%
GAATGTGTGGAGACTACATC+AGG + chr8_1:3863815-3863834 Msa1173470:intron 45.0%
GGAGACTACATCAGGATTGT+AGG + chr8_1:3863823-3863842 Msa1173470:intron 45.0%
CTTTGGATTCTCCTTCCTCT+TGG - chr8_1:3863924-3863943 None:intergenic 45.0%
TACCATCAGGCTCATAGCTT+TGG + chr8_1:3864103-3864122 Msa1173470:intron 45.0%
GGCGGTAATTTGAGAATTGG+TGG + chr8_1:3864150-3864169 Msa1173470:intron 45.0%
!! GATAAAAATAAAATAAAAAA+AGG - chr8_1:3863008-3863027 None:intergenic 5.0%
AACGGAAAAGCCACAGCTGA+CGG - chr8_1:3861913-3861932 None:intergenic 50.0%
! CTCCGCCTTGTTCAGAAGAT+CGG - chr8_1:3862031-3862050 None:intergenic 50.0%
AGCCGATGTGAAGAAGAAGG+AGG + chr8_1:3862062-3862081 Msa1173470:five_prime_UTR 50.0%
GCCGATGTGAAGAAGAAGGA+GGG + chr8_1:3862063-3862082 Msa1173470:five_prime_UTR 50.0%
AGAAGAAGGAGGGACTGAAG+CGG + chr8_1:3862073-3862092 Msa1173470:five_prime_UTR 50.0%
GTCGTCTCAAGCGTTAGAGA+GGG - chr8_1:3862258-3862277 None:intergenic 50.0%
AGTCGTCTCAAGCGTTAGAG+AGG - chr8_1:3862259-3862278 None:intergenic 50.0%
AGAGGAAGCTGACTATGTGG+GGG + chr8_1:3862640-3862659 Msa1173470:CDS 50.0%
TAAGCTAAGGCCGTCGATGA+TGG + chr8_1:3863165-3863184 Msa1173470:intron 50.0%
GGATTCTCCTTCCTCTTGGA+TGG - chr8_1:3863920-3863939 None:intergenic 50.0%
TGCCAAAGCTATGAGCCTGA+TGG - chr8_1:3864108-3864127 None:intergenic 50.0%
! GTTCCAGAGACGGTAGTGGT+GGG - chr8_1:3861852-3861871 None:intergenic 55.0%
! AGTTCCAGAGACGGTAGTGG+TGG - chr8_1:3861853-3861872 None:intergenic 55.0%
GAGAGTTCCAGAGACGGTAG+TGG - chr8_1:3861856-3861875 None:intergenic 55.0%
!! GTGGAGGATGAGATGACGGA+GGG - chr8_1:3861889-3861908 None:intergenic 55.0%
!! CGTTGAAGGTGCTACAACGG+TGG - chr8_1:3861943-3861962 None:intergenic 55.0%
CGATCTTCTGAACAAGGCGG+AGG + chr8_1:3862029-3862048 Msa1173470:five_prime_UTR 55.0%
TCTTCTGAACAAGGCGGAGG+TGG + chr8_1:3862032-3862051 Msa1173470:five_prime_UTR 55.0%
TCTCCCACCACTACCGTCTC+TGG + chr8_1:3861846-3861865 Msa1173470:five_prime_UTR 60.0%
!! GAGATGACGGAGGGAGGCAA+TGG - chr8_1:3861880-3861899 None:intergenic 60.0%
!! GAGGATGAGATGACGGAGGG+AGG - chr8_1:3861886-3861905 None:intergenic 60.0%
! CGTGGAGGATGAGATGACGG+AGG - chr8_1:3861890-3861909 None:intergenic 60.0%
CGGCGTGGAGGATGAGATGA+CGG - chr8_1:3861893-3861912 None:intergenic 60.0%
AAAAGCCACAGCTGACGGCG+TGG - chr8_1:3861908-3861927 None:intergenic 60.0%
GGGACTGAAGCGGCATGTGA+AGG + chr8_1:3862083-3862102 Msa1173470:five_prime_UTR 60.0%
ATCCTCCACGCCGTCAGCTG+TGG + chr8_1:3861900-3861919 Msa1173470:five_prime_UTR 65.0%
AGCCACAGCTGACGGCGTGG+AGG - chr8_1:3861905-3861924 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 3861786 3865211 3861786 ID=Msa1173470;Name=Msa1173470
chr8_1 mRNA 3861786 3865211 3861786 ID=Msa1173470-mRNA-1;Parent=Msa1173470;Name=Msa1173470-mRNA-1;_AED=0.33;_eAED=0.33;_QI=520|0.5|0.6|1|0.25|0.6|5|180|152
chr8_1 exon 3861786 3862389 3861786 ID=Msa1173470-mRNA-1:exon:1377;Parent=Msa1173470-mRNA-1
chr8_1 exon 3862626 3862694 3862626 ID=Msa1173470-mRNA-1:exon:1378;Parent=Msa1173470-mRNA-1
chr8_1 exon 3863174 3863298 3863174 ID=Msa1173470-mRNA-1:exon:1379;Parent=Msa1173470-mRNA-1
chr8_1 exon 3863463 3863495 3863463 ID=Msa1173470-mRNA-1:exon:1380;Parent=Msa1173470-mRNA-1
chr8_1 exon 3864884 3865211 3864884 ID=Msa1173470-mRNA-1:exon:1381;Parent=Msa1173470-mRNA-1
chr8_1 five_prime_UTR 3861786 3862305 3861786 ID=Msa1173470-mRNA-1:five_prime_utr;Parent=Msa1173470-mRNA-1
chr8_1 CDS 3862306 3862389 3862306 ID=Msa1173470-mRNA-1:cds;Parent=Msa1173470-mRNA-1
chr8_1 CDS 3862626 3862694 3862626 ID=Msa1173470-mRNA-1:cds;Parent=Msa1173470-mRNA-1
chr8_1 CDS 3863174 3863298 3863174 ID=Msa1173470-mRNA-1:cds;Parent=Msa1173470-mRNA-1
chr8_1 CDS 3863463 3863495 3863463 ID=Msa1173470-mRNA-1:cds;Parent=Msa1173470-mRNA-1
chr8_1 CDS 3864884 3865031 3864884 ID=Msa1173470-mRNA-1:cds;Parent=Msa1173470-mRNA-1
chr8_1 three_prime_UTR 3865032 3865211 3865032 ID=Msa1173470-mRNA-1:three_prime_utr;Parent=Msa1173470-mRNA-1
Gene Sequence

>Msa1173470

ATGTTCGGGTATTCCCAAAATTGTTCATCTTTTCACTTTCACCTTCTTCTCCGTTTCGATGACTATTCAATTTCGTCGATTGAGCTCGGATATGATGAAGAGGAAGCTGACTATGTGGGGGATGTTTCTAAGTTGACTGATAACAATCCATTGGCCGTCGATGATGGTGTTCCTATTTGCCTATCAATCGTCCTTAAGTGTCTTGCAGCTAAGATAACCGTCGGTCTCCGTTCACATTTATCTGCAATTCCTTTGCACCCCATTCAAATATTTGACATAGTTCATTGGCATACTAGGTTCCTTATCCGAAGGTTATGTGGTTATGCAGTAGCCAAATTCAATATTGTGATGCTTGAAAGAAGAATGATTATGCAGCTTGAAAAAGATGTTCACAAAAAAAGGAGAACTCACATGCTGTCGTGCTGCCACTTTCTTTTAGATCTAGAATATAACCAGTAA
Protein sequence

>Msa1173470

MFGYSQNCSSFHFHLLLRFDDYSISSIELGYDEEEADYVGDVSKLTDNNPLAVDDGVPICLSIVLKCLAAKITVGLRSHLSAIPLHPIQIFDIVHWHTRFLIRRLCGYAVAKFNIVMLERRMIMQLEKDVHKKRRTHMLSCCHFLLDLEYNQ*