Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053340 | XP_013447886.1 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 3.74e-90 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053340 | A0A072TYJ6 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 1.79e-90 | 268 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0034100 | Msa1053340 | -0.826029 | 3.394349e-54 | -8.615850e-47 |
| Msa0040360 | Msa1053340 | 0.804905 | 1.725809e-49 | -8.615850e-47 |
| Msa0606600 | Msa1053340 | 0.828646 | 8.009038e-55 | -8.615850e-47 |
| Msa0610240 | Msa1053340 | -0.806092 | 9.728057e-50 | -8.615850e-47 |
| Msa0647300 | Msa1053340 | 0.822621 | 2.147656e-53 | -8.615850e-47 |
| Msa0659020 | Msa1053340 | -0.800083 | 1.701664e-48 | -8.615850e-47 |
| Msa0685390 | Msa1053340 | -0.803220 | 3.867880e-49 | -8.615850e-47 |
| Msa0686830 | Msa1053340 | 0.826285 | 2.950993e-54 | -8.615850e-47 |
| Msa0703430 | Msa1053340 | -0.806284 | 8.863024e-50 | -8.615850e-47 |
| Msa0705520 | Msa1053340 | 0.800678 | 1.287435e-48 | -8.615850e-47 |
| Msa0708680 | Msa1053340 | 0.814800 | 1.281119e-51 | -8.615850e-47 |
| Msa0731580 | Msa1053340 | 0.805076 | 1.589367e-49 | -8.615850e-47 |
| Msa0756670 | Msa1053340 | -0.815846 | 7.498926e-52 | -8.615850e-47 |
| Msa0836060 | Msa1053340 | -0.806764 | 7.022212e-50 | -8.615850e-47 |
| Msa0120860 | Msa1053340 | 0.817326 | 3.495578e-52 | -8.615850e-47 |
| Msa0128110 | Msa1053340 | -0.812109 | 5.001400e-51 | -8.615850e-47 |
| Msa0128340 | Msa1053340 | 0.804926 | 1.708746e-49 | -8.615850e-47 |
| Msa0128740 | Msa1053340 | 0.802158 | 6.406252e-49 | -8.615850e-47 |
| Msa0136180 | Msa1053340 | -0.807868 | 4.096527e-50 | -8.615850e-47 |
| Msa0166180 | Msa1053340 | 0.801126 | 1.042897e-48 | -8.615850e-47 |
| Msa0180210 | Msa1053340 | 0.801862 | 7.369034e-49 | -8.615850e-47 |
| Msa0186200 | Msa1053340 | 0.820251 | 7.571557e-53 | -8.615850e-47 |
| Msa0190670 | Msa1053340 | 0.802497 | 5.456485e-49 | -8.615850e-47 |
| Msa0214800 | Msa1053340 | 0.809963 | 1.459444e-50 | -8.615850e-47 |
| Msa0373550 | Msa1053340 | 0.811909 | 5.528505e-51 | -8.615850e-47 |
| Msa0468660 | Msa1053340 | 0.813631 | 2.321048e-51 | -8.615850e-47 |
| Msa1011800 | Msa1053340 | 0.949377 | 1.440550e-107 | -8.615850e-47 |
| Msa1011820 | Msa1053340 | 0.825954 | 3.536197e-54 | -8.615850e-47 |
| Msa1011840 | Msa1053340 | 0.826579 | 2.510500e-54 | -8.615850e-47 |
| Msa1011860 | Msa1053340 | 0.838204 | 3.303205e-57 | -8.615850e-47 |
| Msa1011870 | Msa1053340 | 0.893447 | 6.235359e-75 | -8.615850e-47 |
| Msa1030320 | Msa1053340 | 0.810242 | 1.270469e-50 | -8.615850e-47 |
| Msa1053300 | Msa1053340 | 0.818674 | 1.732671e-52 | -8.615850e-47 |
| Msa1053310 | Msa1053340 | 0.945858 | 1.387152e-104 | -8.615850e-47 |
| Msa1053330 | Msa1053340 | 0.856262 | 3.623038e-62 | -8.615850e-47 |
| Msa1053340 | Msa1088400 | -0.818908 | 1.533006e-52 | -8.615850e-47 |
| Msa1053340 | Msa1094810 | 0.800411 | 1.459471e-48 | -8.615850e-47 |
| Msa1053340 | Msa1095400 | 0.830339 | 3.105859e-55 | -8.615850e-47 |
| Msa1053340 | Msa1095410 | 0.951180 | 3.512539e-109 | -8.615850e-47 |
| Msa1053340 | Msa1095430 | 0.802071 | 6.676890e-49 | -8.615850e-47 |
| Msa1053340 | Msa1095440 | 0.804995 | 1.652514e-49 | -8.615850e-47 |
| Msa1053340 | Msa1107330 | 0.807984 | 3.870045e-50 | -8.615850e-47 |
| Msa1053340 | Msa1142570 | 0.839786 | 1.286633e-57 | -8.615850e-47 |
| Msa1053340 | Msa1142580 | 0.839006 | 2.050859e-57 | -8.615850e-47 |
| Msa1053340 | Msa1142590 | 0.983159 | 5.470362e-157 | -8.615850e-47 |
| Msa1053340 | Msa1142600 | 0.828673 | 7.889511e-55 | -8.615850e-47 |
| Msa1053340 | Msa1146980 | 0.841166 | 5.601367e-58 | -8.615850e-47 |
| Msa1053340 | Msa1157210 | 0.820332 | 7.255748e-53 | -8.615850e-47 |
| Msa1053340 | Msa1165460 | 0.802959 | 4.380583e-49 | -8.615850e-47 |
| Msa1053340 | Msa1186670 | 0.806116 | 9.618112e-50 | -8.615850e-47 |
| Msa1053340 | Msa1191350 | 0.812564 | 3.978927e-51 | -8.615850e-47 |
| Msa1053340 | Msa1192340 | 0.805417 | 1.348504e-49 | -8.615850e-47 |
| Msa1053340 | Msa1197720 | 0.848322 | 6.598351e-60 | -8.615850e-47 |
| Msa1053340 | Msa1211130 | 0.820617 | 6.238634e-53 | -8.615850e-47 |
| Msa1053340 | Msa1238250 | 0.804404 | 2.196027e-49 | -8.615850e-47 |
| Msa1053340 | Msa1251410 | 0.823887 | 1.087328e-53 | -8.615850e-47 |
| Msa1053340 | Msa1319030 | 0.808924 | 2.438054e-50 | -8.615850e-47 |
| Msa1053340 | Msa1319190 | 0.812687 | 3.739203e-51 | -8.615850e-47 |
| Msa1053340 | Msa1322500 | 0.814003 | 1.922153e-51 | -8.615850e-47 |
| Msa1053340 | Msa1359590 | 0.804102 | 2.538009e-49 | -8.615850e-47 |
| Msa1053340 | Msa1361980 | 0.809513 | 1.823299e-50 | -8.615850e-47 |
| Msa1053340 | Msa1400290 | -0.826476 | 2.656084e-54 | -8.615850e-47 |
| Msa1053340 | Msa1403660 | 0.810538 | 1.096812e-50 | -8.615850e-47 |
| Msa1053340 | Msa1429810 | 0.819397 | 1.186885e-52 | -8.615850e-47 |
| Msa1053340 | Msa1455360 | 0.800956 | 1.129936e-48 | -8.615850e-47 |
| Msa1053340 | Msa1462780 | 0.828713 | 7.715327e-55 | -8.615850e-47 |
| Msa0242720 | Msa1053340 | 0.817286 | 3.567127e-52 | -8.615850e-47 |
| Msa0294200 | Msa1053340 | 0.801830 | 7.481330e-49 | -8.615850e-47 |
| Msa0491780 | Msa1053340 | 0.800905 | 1.157554e-48 | -8.615850e-47 |
| Msa0514540 | Msa1053340 | 0.802852 | 4.609162e-49 | -8.615850e-47 |
| Msa0522280 | Msa1053340 | 0.823658 | 1.230440e-53 | -8.615850e-47 |
| Msa0850480 | Msa1053340 | 0.801010 | 1.101357e-48 | -8.615850e-47 |
| Msa0882370 | Msa1053340 | 0.823994 | 1.026561e-53 | -8.615850e-47 |
| Msa0900830 | Msa1053340 | 0.825832 | 3.781244e-54 | -8.615850e-47 |
| Msa0926240 | Msa1053340 | 0.842462 | 2.548976e-58 | -8.615850e-47 |
| Msa0932140 | Msa1053340 | 0.819663 | 1.032116e-52 | -8.615850e-47 |
| Msa0932150 | Msa1053340 | 0.830078 | 3.596368e-55 | -8.615850e-47 |
| Msa0936630 | Msa1053340 | 0.800580 | 1.348121e-48 | -8.615850e-47 |
| Msa0965800 | Msa1053340 | 0.840134 | 1.044101e-57 | -8.615850e-47 |
| Msa0970270 | Msa1053340 | 0.812101 | 5.021270e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053340 | MtrunA17_Chr7g0223391 | 95.620 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 7.63e-94 | 267 |
| Msa1053340 | MtrunA17_Chr7g0223401 | 94.891 | 137 | 7 | 0 | 1 | 137 | 6 | 142 | 1.38e-92 | 264 |
| Msa1053340 | MtrunA17_Chr8g0356181 | 47.857 | 140 | 70 | 1 | 1 | 137 | 1 | 140 | 8.69e-41 | 132 |
| Msa1053340 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 5.40e-20 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053340 | AT2G44310.1 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 9.62e-40 | 130 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_2:-22762140 | None:intergenic |
| CTTGAATGGCACAATTGATT+TGG | 0.268459 | 7_2:-22761885 | Msa1053340:CDS |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7_2:+22761938 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_2:+22762095 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_2:-22761804 | Msa1053340:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_2:+22761800 | None:intergenic |
| GAAGCATTTAATAGCTCAGT+TGG | 0.431408 | 7_2:-22762082 | Msa1053340:CDS |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_2:-22762075 | Msa1053340:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_2:+22762101 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_2:-22761810 | Msa1053340:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7_2:+22761939 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7_2:-22761999 | Msa1053340:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7_2:-22761899 | Msa1053340:CDS |
| CTCTTATGATGAGATGTTGA+AGG | 0.511648 | 7_2:-22762017 | Msa1053340:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_2:-22761831 | Msa1053340:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7_2:-22762000 | Msa1053340:CDS |
| ATGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7_2:-22762009 | Msa1053340:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7_2:-22761753 | Msa1053340:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_2:-22761830 | Msa1053340:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558215 | 7_2:-22761836 | Msa1053340:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_2:-22761969 | Msa1053340:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_2:+22762111 | None:intergenic |
| CGCCTCGACACAAACAGCGA+TGG | 0.598011 | 7_2:-22762046 | Msa1053340:CDS |
| AAAGCAGATGATGCTTGCAA+TGG | 0.609930 | 7_2:-22761843 | Msa1053340:CDS |
| GACCATCGCTGTTTGTGTCG+AGG | 0.621315 | 7_2:+22762044 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.625156 | 7_2:-22761835 | Msa1053340:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_2:22761745-22761764 | Msa1053340:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_2:22761789-22761808 | None:intergenic | 30.0% | |
| TTAATAGCTCAGTTGGAAAT+CGG | - | chr7_2:22761806-22761825 | Msa1053340:CDS | 30.0% | |
| ! | TGAAAGAGAGTACAATAAAG+TGG | - | chr7_2:22762128-22762147 | Msa1053340:CDS | 30.0% |
| ! | GAAGCATTTAATAGCTCAGT+TGG | - | chr7_2:22761799-22761818 | Msa1053340:CDS | 35.0% |
| CTCTTATGATGAGATGTTGA+AGG | - | chr7_2:22761864-22761883 | Msa1053340:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_2:22761901-22761920 | Msa1053340:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_2:22761912-22761931 | Msa1053340:CDS | 35.0% |
| CTTGAATGGCACAATTGATT+TGG | - | chr7_2:22761996-22762015 | Msa1053340:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_2:22762084-22762103 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTG+AGG | + | chr7_2:22762116-22762135 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_2:22761773-22761792 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_2:22761783-22761802 | None:intergenic | 40.0% | |
| TTTCAAAAACCCTCAAGCTC+CGG | + | chr7_2:22761894-22761913 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_2:22761982-22762001 | Msa1053340:CDS | 40.0% | |
| AAAGCAGATGATGCTTGCAA+TGG | - | chr7_2:22762038-22762057 | Msa1053340:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_2:22762071-22762090 | Msa1053340:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_2:22762077-22762096 | Msa1053340:CDS | 40.0% | |
| ATGAGATGTTGAAGGAGCTC+CGG | - | chr7_2:22761872-22761891 | Msa1053340:CDS | 45.0% | |
| AAACACGAGCAAGCTCGTTT+GGG | + | chr7_2:22761945-22761964 | None:intergenic | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | + | chr7_2:22761946-22761965 | None:intergenic | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_2:22762045-22762064 | Msa1053340:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | chr7_2:22762046-22762065 | Msa1053340:CDS | 45.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_2:22762051-22762070 | Msa1053340:CDS | 50.0% | |
| GACCATCGCTGTTTGTGTCG+AGG | + | chr7_2:22761840-22761859 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_2:22762050-22762069 | Msa1053340:CDS | 55.0% | |
| CGCCTCGACACAAACAGCGA+TGG | - | chr7_2:22761835-22761854 | Msa1053340:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | - | chr7_2:22761881-22761900 | Msa1053340:CDS | 60.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | - | chr7_2:22761882-22761901 | Msa1053340:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 22761745 | 22762158 | 22761745 | ID=Msa1053340;Name=Msa1053340 |
| chr7_2 | mRNA | 22761745 | 22762158 | 22761745 | ID=Msa1053340-mRNA-1;Parent=Msa1053340;Name=Msa1053340-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_2 | exon | 22761745 | 22762158 | 22761745 | ID=Msa1053340-mRNA-1:exon:5156;Parent=Msa1053340-mRNA-1 |
| chr7_2 | CDS | 22761745 | 22762158 | 22761745 | ID=Msa1053340-mRNA-1:cds;Parent=Msa1053340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
