Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011800 | XP_013447886.1 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 3.74e-90 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011800 | A0A072TYJ6 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 1.79e-90 | 268 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0805390 | Msa1011800 | 0.802713 | 4.923033e-49 | -8.615850e-47 |
| Msa0120860 | Msa1011800 | 0.808806 | 2.584794e-50 | -8.615850e-47 |
| Msa0468660 | Msa1011800 | 0.800076 | 1.707444e-48 | -8.615850e-47 |
| Msa1011800 | Msa1011860 | 0.810572 | 1.078125e-50 | -8.615850e-47 |
| Msa1011800 | Msa1011870 | 0.936665 | 1.207753e-97 | -8.615850e-47 |
| Msa1011800 | Msa1053300 | 0.801856 | 7.392268e-49 | -8.615850e-47 |
| Msa1011800 | Msa1053310 | 0.902191 | 1.247702e-78 | -8.615850e-47 |
| Msa1011800 | Msa1053330 | 0.823022 | 1.732583e-53 | -8.615850e-47 |
| Msa1011800 | Msa1053340 | 0.949377 | 1.440550e-107 | -8.615850e-47 |
| Msa1011800 | Msa1095400 | 0.812884 | 3.385693e-51 | -8.615850e-47 |
| Msa1011800 | Msa1095410 | 0.900363 | 7.893817e-78 | -8.615850e-47 |
| Msa1011800 | Msa1095430 | 0.806183 | 9.307436e-50 | -8.615850e-47 |
| Msa1011800 | Msa1142580 | 0.824443 | 8.050710e-54 | -8.615850e-47 |
| Msa1011800 | Msa1142590 | 0.926750 | 3.050819e-91 | -8.615850e-47 |
| Msa1011800 | Msa1146980 | 0.815936 | 7.160714e-52 | -8.615850e-47 |
| Msa1011800 | Msa1197720 | 0.841357 | 4.992234e-58 | -8.615850e-47 |
| Msa1011800 | Msa1251410 | 0.834355 | 3.145087e-56 | -8.615850e-47 |
| Msa1011800 | Msa1403660 | 0.800526 | 1.383183e-48 | -8.615850e-47 |
| Msa0900830 | Msa1011800 | 0.810007 | 1.427447e-50 | -8.615850e-47 |
| Msa0926240 | Msa1011800 | 0.827960 | 1.171820e-54 | -8.615850e-47 |
| Msa0965800 | Msa1011800 | 0.815800 | 7.679736e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011800 | MtrunA17_Chr7g0223391 | 95.620 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 7.63e-94 | 267 |
| Msa1011800 | MtrunA17_Chr7g0223401 | 94.891 | 137 | 7 | 0 | 1 | 137 | 6 | 142 | 1.38e-92 | 264 |
| Msa1011800 | MtrunA17_Chr8g0356181 | 47.857 | 140 | 70 | 1 | 1 | 137 | 1 | 140 | 8.69e-41 | 132 |
| Msa1011800 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 5.40e-20 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011800 | AT2G44310.1 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 9.62e-40 | 130 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_1:-16590201 | None:intergenic |
| CTTGAATGGCACAATTGATT+TGG | 0.268459 | 7_1:-16589946 | Msa1011800:CDS |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7_1:+16589999 | None:intergenic |
| TAAATGCTTCTTCGTCTTCA+AGG | 0.385959 | 7_1:+16590156 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_1:-16589865 | Msa1011800:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_1:+16589861 | None:intergenic |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_1:-16590136 | Msa1011800:CDS |
| GAAGCATTTAATAGCTCAGT+TGG | 0.438400 | 7_1:-16590143 | Msa1011800:CDS |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_1:-16589871 | Msa1011800:CDS |
| CTTCTTCGTCTTCAAGGAAG+TGG | 0.458207 | 7_1:+16590162 | None:intergenic |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7_1:+16590000 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7_1:-16590060 | Msa1011800:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7_1:-16589960 | Msa1011800:CDS |
| CTCTTATGATGAGATGTTGA+AGG | 0.511648 | 7_1:-16590078 | Msa1011800:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_1:-16589892 | Msa1011800:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7_1:-16590061 | Msa1011800:CDS |
| ATGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7_1:-16590070 | Msa1011800:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7_1:-16589814 | Msa1011800:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_1:-16589891 | Msa1011800:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558215 | 7_1:-16589897 | Msa1011800:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_1:-16590030 | Msa1011800:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_1:+16590172 | None:intergenic |
| CGCCTCGACACAAACAGCGA+TGG | 0.598011 | 7_1:-16590107 | Msa1011800:CDS |
| AAAGCAGATGATGCTTGCAA+TGG | 0.609930 | 7_1:-16589904 | Msa1011800:CDS |
| GACCATCGCTGTTTGTGTCG+AGG | 0.621315 | 7_1:+16590105 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.625156 | 7_1:-16589896 | Msa1011800:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_1:16589806-16589825 | Msa1011800:CDS | 30.0% |
| TTAATAGCTCAGTTGGAAAT+CGG | - | chr7_1:16589867-16589886 | Msa1011800:CDS | 30.0% | |
| ! | TGAAAGAGAGTACAATAAAG+TGG | - | chr7_1:16590189-16590208 | Msa1011800:CDS | 30.0% |
| TAAATGCTTCTTCGTCTTCA+AGG | + | chr7_1:16589850-16589869 | None:intergenic | 35.0% | |
| ! | GAAGCATTTAATAGCTCAGT+TGG | - | chr7_1:16589860-16589879 | Msa1011800:CDS | 35.0% |
| CTCTTATGATGAGATGTTGA+AGG | - | chr7_1:16589925-16589944 | Msa1011800:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_1:16589962-16589981 | Msa1011800:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_1:16589973-16589992 | Msa1011800:CDS | 35.0% |
| CTTGAATGGCACAATTGATT+TGG | - | chr7_1:16590057-16590076 | Msa1011800:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_1:16590145-16590164 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTG+AGG | + | chr7_1:16590177-16590196 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_1:16589834-16589853 | None:intergenic | 40.0% | |
| TTTCAAAAACCCTCAAGCTC+CGG | + | chr7_1:16589955-16589974 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_1:16590043-16590062 | Msa1011800:CDS | 40.0% | |
| AAAGCAGATGATGCTTGCAA+TGG | - | chr7_1:16590099-16590118 | Msa1011800:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_1:16590132-16590151 | Msa1011800:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_1:16590138-16590157 | Msa1011800:CDS | 40.0% | |
| CTTCTTCGTCTTCAAGGAAG+TGG | + | chr7_1:16589844-16589863 | None:intergenic | 45.0% | |
| ATGAGATGTTGAAGGAGCTC+CGG | - | chr7_1:16589933-16589952 | Msa1011800:CDS | 45.0% | |
| AAACACGAGCAAGCTCGTTT+GGG | + | chr7_1:16590006-16590025 | None:intergenic | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | + | chr7_1:16590007-16590026 | None:intergenic | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_1:16590106-16590125 | Msa1011800:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | chr7_1:16590107-16590126 | Msa1011800:CDS | 45.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_1:16590112-16590131 | Msa1011800:CDS | 50.0% | |
| GACCATCGCTGTTTGTGTCG+AGG | + | chr7_1:16589901-16589920 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_1:16590111-16590130 | Msa1011800:CDS | 55.0% | |
| CGCCTCGACACAAACAGCGA+TGG | - | chr7_1:16589896-16589915 | Msa1011800:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | - | chr7_1:16589942-16589961 | Msa1011800:CDS | 60.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | - | chr7_1:16589943-16589962 | Msa1011800:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 16589806 | 16590219 | 16589806 | ID=Msa1011800;Name=Msa1011800 |
| chr7_1 | mRNA | 16589806 | 16590219 | 16589806 | ID=Msa1011800-mRNA-1;Parent=Msa1011800;Name=Msa1011800-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_1 | exon | 16589806 | 16590219 | 16589806 | ID=Msa1011800-mRNA-1:exon:4231;Parent=Msa1011800-mRNA-1 |
| chr7_1 | CDS | 16589806 | 16590219 | 16589806 | ID=Msa1011800-mRNA-1:cds;Parent=Msa1011800-mRNA-1 |
| Gene Sequence |
| Protein sequence |
