Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053330 | XP_013447886.1 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 5.95e-89 | 265 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053330 | A0A072TYJ6 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 2.84e-89 | 265 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011790 | Msa1053330 | 0.828598 | 8.225064e-55 | -8.615850e-47 |
| Msa1011800 | Msa1053330 | 0.823022 | 1.732583e-53 | -8.615850e-47 |
| Msa1011810 | Msa1053330 | 0.807308 | 5.386381e-50 | -8.615850e-47 |
| Msa1011820 | Msa1053330 | 0.893179 | 7.997397e-75 | -8.615850e-47 |
| Msa1011830 | Msa1053330 | 0.800966 | 1.124849e-48 | -8.615850e-47 |
| Msa1011840 | Msa1053330 | 0.888199 | 7.294847e-73 | -8.615850e-47 |
| Msa1011860 | Msa1053330 | 0.858306 | 9.016347e-63 | -8.615850e-47 |
| Msa1053290 | Msa1053330 | 0.840388 | 8.963223e-58 | -8.615850e-47 |
| Msa1053310 | Msa1053330 | 0.829749 | 4.325785e-55 | -8.615850e-47 |
| Msa1053330 | Msa1053340 | 0.856262 | 3.623038e-62 | -8.615850e-47 |
| Msa1053330 | Msa1095400 | 0.809473 | 1.859384e-50 | -8.615850e-47 |
| Msa1053330 | Msa1095410 | 0.828563 | 8.388257e-55 | -8.615850e-47 |
| Msa1053330 | Msa1095430 | 0.835019 | 2.140126e-56 | -8.615850e-47 |
| Msa1053330 | Msa1095440 | 0.845235 | 4.608961e-59 | -8.615850e-47 |
| Msa1053330 | Msa1142590 | 0.856700 | 2.694203e-62 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053330 | MtrunA17_Chr7g0223391 | 95.620 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 1.10e-92 | 265 |
| Msa1053330 | MtrunA17_Chr7g0223401 | 90.511 | 137 | 13 | 0 | 1 | 137 | 6 | 142 | 4.35e-89 | 255 |
| Msa1053330 | MtrunA17_Chr8g0356181 | 49.286 | 140 | 68 | 1 | 1 | 137 | 1 | 140 | 3.66e-42 | 136 |
| Msa1053330 | MtrunA17_Chr1g0190921 | 35.075 | 134 | 82 | 1 | 1 | 134 | 1 | 129 | 6.09e-20 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053330 | AT2G44310.1 | 49.635 | 137 | 67 | 1 | 1 | 135 | 1 | 137 | 1.10e-41 | 135 |
Find 28 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_2:-22757704 | None:intergenic |
| CGGTTGATCTTGAAGAATTC+AGG | 0.278025 | 7_2:-22757438 | Msa1053330:CDS |
| TGAAAGAGAGTTCAGTAAAT+TGG | 0.290880 | 7_2:-22757317 | Msa1053330:CDS |
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_2:+22757502 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_2:+22757659 | None:intergenic |
| TCTTCCAAGACCATTTGAAT+AGG | 0.402040 | 7_2:+22757364 | None:intergenic |
| CATGCCTATTCAAATGGTCT+TGG | 0.421269 | 7_2:-22757368 | Msa1053330:CDS |
| CAGTTTGATCACAACTTGAA+TGG | 0.425360 | 7_2:-22757463 | Msa1053330:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_2:+22757665 | None:intergenic |
| GGGTTTCATGCCTATTCAAA+TGG | 0.450103 | 7_2:-22757374 | Msa1053330:CDS |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.454217 | 7_2:-22757639 | Msa1053330:CDS |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_2:+22757579 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_2:+22757503 | None:intergenic |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_2:-22757581 | Msa1053330:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.520375 | 7_2:-22757563 | Msa1053330:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_2:-22757395 | Msa1053330:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7_2:-22757646 | Msa1053330:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.545635 | 7_2:-22757400 | Msa1053330:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7_2:-22757650 | Msa1053330:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_2:-22757394 | Msa1053330:CDS |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.558625 | 7_2:-22757564 | Msa1053330:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_2:-22757533 | Msa1053330:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_2:+22757675 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_2:-22757407 | Msa1053330:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.619568 | 7_2:-22757399 | Msa1053330:CDS |
| TGATCACAACTTGAATGGCA+CGG | 0.635410 | 7_2:-22757458 | Msa1053330:CDS |
| TGATCTTGAAGAATTCAGGA+AGG | 0.643588 | 7_2:-22757434 | Msa1053330:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_2:-22757610 | Msa1053330:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_2:22757309-22757328 | Msa1053330:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_2:22757353-22757372 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+CGG | - | chr7_2:22757359-22757378 | Msa1053330:CDS | 30.0% |
| TGAAAGAGAGTTCAGTAAAT+TGG | - | chr7_2:22757692-22757711 | Msa1053330:CDS | 30.0% | |
| TTAATAGCTCGGTAGGAAAT+CGG | - | chr7_2:22757370-22757389 | Msa1053330:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_2:22757465-22757484 | Msa1053330:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_2:22757476-22757495 | Msa1053330:CDS | 35.0% |
| CAGTTTGATCACAACTTGAA+TGG | - | chr7_2:22757546-22757565 | Msa1053330:CDS | 35.0% | |
| TGATCTTGAAGAATTCAGGA+AGG | - | chr7_2:22757575-22757594 | Msa1053330:CDS | 35.0% | |
| TAAGCAAATGATGCTTGCAA+TGG | - | chr7_2:22757602-22757621 | Msa1053330:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+AGG | + | chr7_2:22757648-22757667 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTC+AGG | + | chr7_2:22757680-22757699 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_2:22757337-22757356 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_2:22757347-22757366 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | - | chr7_2:22757363-22757382 | Msa1053330:CDS | 40.0% |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_2:22757428-22757447 | Msa1053330:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_2:22757433-22757452 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_2:22757509-22757528 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_2:22757510-22757529 | None:intergenic | 40.0% | |
| TGATCACAACTTGAATGGCA+CGG | - | chr7_2:22757551-22757570 | Msa1053330:CDS | 40.0% | |
| CGGTTGATCTTGAAGAATTC+AGG | - | chr7_2:22757571-22757590 | Msa1053330:CDS | 40.0% | |
| GGGTTTCATGCCTATTCAAA+TGG | - | chr7_2:22757635-22757654 | Msa1053330:CDS | 40.0% | |
| CATGCCTATTCAAATGGTCT+TGG | - | chr7_2:22757641-22757660 | Msa1053330:CDS | 40.0% | |
| TGCCTCGACACAAATAACGA+CGG | - | chr7_2:22757399-22757418 | Msa1053330:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_2:22757609-22757628 | Msa1053330:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | chr7_2:22757610-22757629 | Msa1053330:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | + | chr7_2:22757404-22757423 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | - | chr7_2:22757445-22757464 | Msa1053330:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | - | chr7_2:22757446-22757465 | Msa1053330:CDS | 50.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_2:22757615-22757634 | Msa1053330:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_2:22757614-22757633 | Msa1053330:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 22757309 | 22757722 | 22757309 | ID=Msa1053330;Name=Msa1053330 |
| chr7_2 | mRNA | 22757309 | 22757722 | 22757309 | ID=Msa1053330-mRNA-1;Parent=Msa1053330;Name=Msa1053330-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_2 | exon | 22757309 | 22757722 | 22757309 | ID=Msa1053330-mRNA-1:exon:5155;Parent=Msa1053330-mRNA-1 |
| chr7_2 | CDS | 22757309 | 22757722 | 22757309 | ID=Msa1053330-mRNA-1:cds;Parent=Msa1053330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
