Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053310 | XP_013447886.1 | 90.551 | 127 | 4 | 1 | 1 | 119 | 1 | 127 | 2.68e-75 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053310 | A0A072TYJ6 | 90.551 | 127 | 4 | 1 | 1 | 119 | 1 | 127 | 1.28e-75 | 229 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0072440 | Msa1053310 | 0.801514 | 8.688330e-49 | -8.615850e-47 |
| Msa0101530 | Msa1053310 | 0.820531 | 6.531169e-53 | -8.615850e-47 |
| Msa0606600 | Msa1053310 | 0.828014 | 1.137681e-54 | -8.615850e-47 |
| Msa0647300 | Msa1053310 | 0.818144 | 2.284272e-52 | -8.615850e-47 |
| Msa0686830 | Msa1053310 | 0.821140 | 4.730434e-53 | -8.615850e-47 |
| Msa0120860 | Msa1053310 | 0.815564 | 8.666379e-52 | -8.615850e-47 |
| Msa0219280 | Msa1053310 | 0.810056 | 1.393340e-50 | -8.615850e-47 |
| Msa0384590 | Msa1053310 | 0.809852 | 1.541626e-50 | -8.615850e-47 |
| Msa1011800 | Msa1053310 | 0.902191 | 1.247702e-78 | -8.615850e-47 |
| Msa1011860 | Msa1053310 | 0.802370 | 5.794714e-49 | -8.615850e-47 |
| Msa1011870 | Msa1053310 | 0.889037 | 3.466094e-73 | -8.615850e-47 |
| Msa1053310 | Msa1053330 | 0.829749 | 4.325785e-55 | -8.615850e-47 |
| Msa1053310 | Msa1053340 | 0.945858 | 1.387152e-104 | -8.615850e-47 |
| Msa1053310 | Msa1088400 | -0.808041 | 3.763413e-50 | -8.615850e-47 |
| Msa1053310 | Msa1094810 | 0.803599 | 3.228364e-49 | -8.615850e-47 |
| Msa1053310 | Msa1095400 | 0.849031 | 4.197047e-60 | -8.615850e-47 |
| Msa1053310 | Msa1095410 | 0.915084 | 8.952563e-85 | -8.615850e-47 |
| Msa1053310 | Msa1095430 | 0.802542 | 5.338770e-49 | -8.615850e-47 |
| Msa1053310 | Msa1095440 | 0.813764 | 2.169477e-51 | -8.615850e-47 |
| Msa1053310 | Msa1142570 | 0.834779 | 2.460505e-56 | -8.615850e-47 |
| Msa1053310 | Msa1142580 | 0.832778 | 7.786437e-56 | -8.615850e-47 |
| Msa1053310 | Msa1142590 | 0.946170 | 7.687067e-105 | -8.615850e-47 |
| Msa1053310 | Msa1142600 | 0.816605 | 5.073515e-52 | -8.615850e-47 |
| Msa1053310 | Msa1146980 | 0.820800 | 5.662923e-53 | -8.615850e-47 |
| Msa1053310 | Msa1197720 | 0.849857 | 2.470847e-60 | -8.615850e-47 |
| Msa1053310 | Msa1251410 | 0.829861 | 4.061210e-55 | -8.615850e-47 |
| Msa1053310 | Msa1322500 | 0.801828 | 7.488555e-49 | -8.615850e-47 |
| Msa1053310 | Msa1359590 | 0.801371 | 9.294847e-49 | -8.615850e-47 |
| Msa1053310 | Msa1400290 | -0.808701 | 2.721426e-50 | -8.615850e-47 |
| Msa1053310 | Msa1403660 | 0.802803 | 4.717087e-49 | -8.615850e-47 |
| Msa1053310 | Msa1462780 | 0.800522 | 1.385798e-48 | -8.615850e-47 |
| Msa0242720 | Msa1053310 | 0.807624 | 4.616131e-50 | -8.615850e-47 |
| Msa0900830 | Msa1053310 | 0.808085 | 3.682637e-50 | -8.615850e-47 |
| Msa0926240 | Msa1053310 | 0.821187 | 4.613434e-53 | -8.615850e-47 |
| Msa0965800 | Msa1053310 | 0.820335 | 7.243149e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053310 | MtrunA17_Chr7g0223391 | 90.551 | 127 | 4 | 1 | 1 | 119 | 18 | 144 | 4.95e-79 | 229 |
| Msa1053310 | MtrunA17_Chr7g0223401 | 88.976 | 127 | 6 | 1 | 1 | 119 | 6 | 132 | 8.64e-77 | 223 |
| Msa1053310 | MtrunA17_Chr8g0356181 | 46.281 | 121 | 57 | 1 | 1 | 113 | 1 | 121 | 6.25e-33 | 112 |
| Msa1053310 | MtrunA17_Chr1g0190921 | 36.975 | 119 | 72 | 2 | 1 | 116 | 1 | 119 | 8.88e-14 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053310 | AT2G44310.1 | 46.281 | 121 | 57 | 1 | 1 | 113 | 1 | 121 | 3.67e-33 | 113 |
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_2:-22744765 | None:intergenic |
| CTTGAATGGCACAATTGATT+TGG | 0.268459 | 7_2:-22744510 | Msa1053310:CDS |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7_2:+22744563 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_2:+22744720 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_2:-22744429 | Msa1053310:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_2:+22744425 | None:intergenic |
| GAAGCATTTAATAGCTCAGT+TGG | 0.431408 | 7_2:-22744707 | Msa1053310:CDS |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_2:-22744700 | Msa1053310:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_2:+22744726 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_2:-22744435 | Msa1053310:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7_2:+22744564 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7_2:-22744624 | Msa1053310:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7_2:-22744524 | Msa1053310:CDS |
| CTCTTATGATGAGATGTTGA+AGG | 0.511648 | 7_2:-22744642 | Msa1053310:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_2:-22744456 | Msa1053310:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7_2:-22744625 | Msa1053310:CDS |
| ATGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7_2:-22744634 | Msa1053310:CDS |
| AACACACTTTGGTATGGATG+TGG | 0.528157 | 7_2:-22744594 | Msa1053310:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_2:-22744455 | Msa1053310:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558215 | 7_2:-22744461 | Msa1053310:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_2:+22744736 | None:intergenic |
| CGCCTCGACACAAACAGCGA+TGG | 0.598011 | 7_2:-22744671 | Msa1053310:CDS |
| AAAGCAGATGATGCTTGCAA+TGG | 0.609930 | 7_2:-22744468 | Msa1053310:CDS |
| GACCATCGCTGTTTGTGTCG+AGG | 0.621315 | 7_2:+22744669 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.625156 | 7_2:-22744460 | Msa1053310:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_2:22744403-22744422 | Msa1053310:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_2:22744447-22744466 | None:intergenic | 30.0% | |
| TTAATAGCTCAGTTGGAAAT+CGG | - | chr7_2:22744464-22744483 | Msa1053310:CDS | 30.0% | |
| !!! | TTTTGAAACACACTTTGGTA+TGG | - | chr7_2:22744564-22744583 | Msa1053310:intron | 30.0% |
| ! | GAAGCATTTAATAGCTCAGT+TGG | - | chr7_2:22744457-22744476 | Msa1053310:CDS | 35.0% |
| CTCTTATGATGAGATGTTGA+AGG | - | chr7_2:22744522-22744541 | Msa1053310:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_2:22744559-22744578 | Msa1053310:intron | 35.0% |
| CTTGAATGGCACAATTGATT+TGG | - | chr7_2:22744654-22744673 | Msa1053310:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_2:22744742-22744761 | None:intergenic | 35.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_2:22744431-22744450 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_2:22744441-22744460 | None:intergenic | 40.0% | |
| TTTCAAAAACCCTCAAGCTC+CGG | + | chr7_2:22744552-22744571 | None:intergenic | 40.0% | |
| AACACACTTTGGTATGGATG+TGG | - | chr7_2:22744570-22744589 | Msa1053310:CDS | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_2:22744640-22744659 | Msa1053310:CDS | 40.0% | |
| AAAGCAGATGATGCTTGCAA+TGG | - | chr7_2:22744696-22744715 | Msa1053310:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_2:22744729-22744748 | Msa1053310:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_2:22744735-22744754 | Msa1053310:CDS | 40.0% | |
| ATGAGATGTTGAAGGAGCTC+CGG | - | chr7_2:22744530-22744549 | Msa1053310:intron | 45.0% | |
| AAACACGAGCAAGCTCGTTT+GGG | + | chr7_2:22744603-22744622 | None:intergenic | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | + | chr7_2:22744604-22744623 | None:intergenic | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_2:22744703-22744722 | Msa1053310:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | chr7_2:22744704-22744723 | Msa1053310:CDS | 45.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_2:22744709-22744728 | Msa1053310:CDS | 50.0% | |
| GACCATCGCTGTTTGTGTCG+AGG | + | chr7_2:22744498-22744517 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_2:22744708-22744727 | Msa1053310:CDS | 55.0% | |
| CGCCTCGACACAAACAGCGA+TGG | - | chr7_2:22744493-22744512 | Msa1053310:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | - | chr7_2:22744539-22744558 | Msa1053310:intron | 60.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | - | chr7_2:22744540-22744559 | Msa1053310:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 22744403 | 22744783 | 22744403 | ID=Msa1053310;Name=Msa1053310 |
| chr7_2 | mRNA | 22744403 | 22744783 | 22744403 | ID=Msa1053310-mRNA-1;Parent=Msa1053310;Name=Msa1053310-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|0|0|2|0|119 |
| chr7_2 | exon | 22744568 | 22744783 | 22744568 | ID=Msa1053310-mRNA-1:exon:5165;Parent=Msa1053310-mRNA-1 |
| chr7_2 | exon | 22744403 | 22744543 | 22744403 | ID=Msa1053310-mRNA-1:exon:5164;Parent=Msa1053310-mRNA-1 |
| chr7_2 | CDS | 22744568 | 22744783 | 22744568 | ID=Msa1053310-mRNA-1:cds;Parent=Msa1053310-mRNA-1 |
| chr7_2 | CDS | 22744403 | 22744543 | 22744403 | ID=Msa1053310-mRNA-1:cds;Parent=Msa1053310-mRNA-1 |
| Gene Sequence |
| Protein sequence |
