AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1114360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1114360 MtrunA17_Chr7g0256981 93.417 319 16 2 1 319 1 314 0.0 534
Msa1114360 MtrunA17_Chr7g0257031 75.938 320 66 4 1 319 1 310 1.59e-144 409
Msa1114360 MtrunA17_Chr7g0257011 68.652 319 67 6 1 319 1 286 7.88e-130 371
Msa1114360 MtrunA17_Chr7g0256971 72.800 250 61 3 34 281 3 247 1.26e-120 347
Msa1114360 MtrunA17_Chr1g0172581 44.751 181 79 6 64 233 1 171 5.02e-39 136
Msa1114360 MtrunA17_Chr3g0085991 42.623 122 69 1 22 142 21 142 3.11e-31 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1114360 AT5G03990.1 34.839 310 155 8 1 305 1 268 4.39e-39 140
Msa1114360 AT3G51940.1 47.619 105 54 1 25 128 20 124 1.18e-30 120
Msa1114360 AT3G51940.2 52.174 92 43 1 38 128 37 128 2.74e-30 119
Msa1114360 AT5G03990.2 32.645 242 123 6 64 305 1 202 9.81e-22 92.4

Find 115 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATCCCAATGTGGTAAAT+TGG 0.166892 7_3:-61179406 Msa1114360:CDS
CAAAGGTGGCTTTCCTTTAT+AGG 0.179989 7_3:+61180920 None:intergenic
GAAATGGCGGTAATGGAAAT+TGG 0.216567 7_3:-61178875 Msa1114360:CDS
TAATTACTGAAATGGATAAT+TGG 0.271436 7_3:-61181001 Msa1114360:exon
TGTCGACCCCGAACTTTATC+TGG 0.292705 7_3:-61179252 Msa1114360:CDS
GGCGAGGCAGCCGTGATTCT+TGG 0.305461 7_3:-61179185 Msa1114360:CDS
GGAGGAGGAGGAAGAGGTGG+AGG 0.309961 7_3:-61178726 Msa1114360:CDS
GGAGGAGGAAGAGGTGGAGG+AGG 0.311785 7_3:-61178723 Msa1114360:CDS
GTTGAACCTTCAAAGGAATA+TGG 0.318838 7_3:-61178981 Msa1114360:CDS
CAGATGGCTTTATTGTCTAT+TGG 0.319056 7_3:-61178486 Msa1114360:three_prime_UTR
GGTGGAGGAGGAGGAAGGAG+GGG 0.319614 7_3:-61178711 Msa1114360:CDS
AGGTGGAGGAGGAGGAAGGA+GGG 0.326176 7_3:-61178712 Msa1114360:CDS
CGCCGCAACATGGAAGAAAA+AGG 0.329418 7_3:-61179206 Msa1114360:CDS
GGAGGAAGAGGTGGAGGAGG+AGG 0.330303 7_3:-61178720 Msa1114360:CDS
TGACCCTTTAGCAGCTTCAT+AGG 0.335346 7_3:+61178606 None:intergenic
CGCCAACCATATTCCTTTGA+AGG 0.340454 7_3:+61178975 None:intergenic
GGATGGGGACCTACTGGATG+GGG 0.344597 7_3:-61179125 Msa1114360:CDS
GGAGGAAGAGGAGGAGGAGG+AGG 0.345038 7_3:-61178738 Msa1114360:CDS
TCCATCCATGATAATCATTC+TGG 0.346408 7_3:+61178946 None:intergenic
GAGGTGGAGGAGGAGGAAGG+AGG 0.349043 7_3:-61178713 Msa1114360:CDS
AAATGGCGGTAATGGAAATT+GGG 0.360900 7_3:-61178874 Msa1114360:CDS
AGAAGAAGAAGCGGAGGAAG+AGG 0.362675 7_3:-61178750 Msa1114360:CDS
AGCGGAGGAAGAGGAGGAGG+AGG 0.362993 7_3:-61178741 Msa1114360:CDS
GATGGGGACCTACTGGATGG+GGG 0.369719 7_3:-61179124 Msa1114360:CDS
AAATCCTCATCGTCCTATAA+AGG 0.372642 7_3:-61180933 Msa1114360:CDS
ATTATATCATATAAGAAGTA+TGG 0.374963 7_3:-61178536 Msa1114360:three_prime_UTR
TCCTTTGAAGGTTCAACAAC+AGG 0.379188 7_3:+61178987 None:intergenic
TTCCACATCCAGATAAAGTT+CGG 0.380635 7_3:+61179244 None:intergenic
TCCACATCCAGATAAAGTTC+GGG 0.382465 7_3:+61179245 None:intergenic
AAAGAGAAAGACACAGATTT+CGG 0.387442 7_3:+61181141 None:intergenic
CTATAAAGGAAAGCCACCTT+TGG 0.390395 7_3:-61180919 Msa1114360:CDS
AAGGAATATGGTTGGCGAAA+TGG 0.391675 7_3:-61178969 Msa1114360:CDS
CTGGATGGGGACCTACTGGA+TGG 0.396986 7_3:-61179127 Msa1114360:CDS
GAAGAGGTGGAGGAGGAGGA+AGG 0.407371 7_3:-61178716 Msa1114360:CDS
CAAGGAAGAAATGGCGGTAA+TGG 0.409545 7_3:-61178882 Msa1114360:CDS
TGGATGGGGACCTACTGGAT+GGG 0.411862 7_3:-61179126 Msa1114360:CDS
AGAAGCGGAGGAAGAGGAGG+AGG 0.414895 7_3:-61178744 Msa1114360:CDS
AACAAGTATCAAGGAAGAAA+TGG 0.421942 7_3:-61178891 Msa1114360:CDS
TCATCCCAATTTACCACATT+GGG 0.428459 7_3:+61179401 None:intergenic
AATTAAGAAACTTTGGGAAA+AGG 0.430371 7_3:+61181100 None:intergenic
AAAATAATGAAACAAACTCT+TGG 0.438543 7_3:-61179025 Msa1114360:CDS
TGGAATCAGACGGAGCAATA+TGG 0.440258 7_3:-61178927 Msa1114360:CDS
GGTTTCCAACAGTGCCTTTG+TGG 0.441064 7_3:-61179502 Msa1114360:CDS
ATCATCATCAAGTGGGTCAC+TGG 0.445627 7_3:-61180956 Msa1114360:CDS
TCCCCATCCAGGACCGGAAA+AGG 0.450165 7_3:+61179139 None:intergenic
ATGGGGACCTACTGGATGGG+GGG 0.451326 7_3:-61179123 Msa1114360:CDS
CCATGGCGAAAGGTAATAGA+AGG 0.451419 7_3:-61179446 Msa1114360:CDS
GGTCCTGGATGGGGACCTAC+TGG 0.453239 7_3:-61179131 Msa1114360:CDS
CTCACATTGATGATGTAGAT+TGG 0.455451 7_3:-61179283 Msa1114360:CDS
TGAACAGAATTATGCTGCTC+AGG 0.456904 7_3:-61179069 Msa1114360:CDS
TCAATGTGAGTGTCGGGATC+AGG 0.460586 7_3:+61179296 None:intergenic
TAAATGAAGATCAAGTGCTT+TGG 0.464392 7_3:-61178645 Msa1114360:three_prime_UTR
ATTAGTGCCATGATGATGTC+CGG 0.466379 7_3:+61178794 None:intergenic
GATGATGATTACTGATCCCT+TGG 0.467657 7_3:+61180970 None:intergenic
AGAATTATGCTGCTCAGGAA+TGG 0.467763 7_3:-61179064 Msa1114360:CDS
TATAAAGGAAAGCCACCTTT+GGG 0.476143 7_3:-61180918 Msa1114360:intron
AAATAATGAAACAAACTCTT+GGG 0.477297 7_3:-61179024 Msa1114360:CDS
TCAACAACAGGAGCACGATA+TGG 0.478493 7_3:+61178999 None:intergenic
GAATTATGCTGCTCAGGAAT+GGG 0.478819 7_3:-61179063 Msa1114360:CDS
AATGAATATCAGCTGAATAG+AGG 0.480106 7_3:-61178774 Msa1114360:CDS
ACATCATCAATGTGAGTGTC+GGG 0.481617 7_3:+61179290 None:intergenic
AGAAGAAGCGGAGGAAGAGG+AGG 0.489710 7_3:-61178747 Msa1114360:CDS
GACTCCTATGAAGCTGCTAA+AGG 0.497038 7_3:-61178610 Msa1114360:three_prime_UTR
GCATCCCAATGTGGTAAATT+GGG 0.497460 7_3:-61179405 Msa1114360:CDS
TCCTGTTGTTGAACCTTCAA+AGG 0.497931 7_3:-61178988 Msa1114360:CDS
ACTCCTATGAAGCTGCTAAA+GGG 0.501415 7_3:-61178609 Msa1114360:three_prime_UTR
GTAATGGAAATTGGGGAGCA+TGG 0.502202 7_3:-61178866 Msa1114360:CDS
GTTTCCAACAGTGCCTTTGT+GGG 0.503784 7_3:-61179501 Msa1114360:CDS
GGAAATTGGGGAGCATGGGA+TGG 0.507278 7_3:-61178861 Msa1114360:CDS
ATCAGGCAATGGTATGTCGC+AGG 0.507842 7_3:+61179313 None:intergenic
TTAGTGCCATGATGATGTCC+GGG 0.507899 7_3:+61178795 None:intergenic
CATCCAGTAGGTCCCCATCC+AGG 0.508679 7_3:+61179128 None:intergenic
TTTCGGAGATAATTATATGA+AGG 0.513750 7_3:+61181158 None:intergenic
TACATCATCAATGTGAGTGT+CGG 0.513899 7_3:+61179289 None:intergenic
ATGATTCAGTACATTAGTAC+TGG 0.514710 7_3:-61178425 Msa1114360:three_prime_UTR
AGAGGAAGAAGAAGAAGCGG+AGG 0.514759 7_3:-61178756 Msa1114360:CDS
ATATGAAGGTGATGAGAAGA+AGG 0.520437 7_3:+61181172 None:intergenic
AATCGATTGGCTCAGTTCCA+TGG 0.520978 7_3:-61179463 Msa1114360:CDS
AGCAGAGGCCCGCCGCAACA+TGG 0.520992 7_3:-61179216 Msa1114360:CDS
AGTACTAATGTACTGAATCA+TGG 0.528010 7_3:+61178427 None:intergenic
GTCATCCCAATTTACCACAT+TGG 0.528139 7_3:+61179400 None:intergenic
TAATGGAAATTGGGGAGCAT+GGG 0.534866 7_3:-61178865 Msa1114360:CDS
CCCCGAACTTTATCTGGATG+TGG 0.535767 7_3:-61179246 Msa1114360:CDS
GTGGAAAAGGTAGCTACTCC+TGG 0.538528 7_3:-61178675 Msa1114360:CDS
CCTTCTATTACCTTTCGCCA+TGG 0.539041 7_3:+61179446 None:intergenic
ATCTTCATTTACCATGCACC+AGG 0.539123 7_3:+61178657 None:intergenic
GCCAGAATGATTATCATGGA+TGG 0.543243 7_3:-61178947 Msa1114360:CDS
GACTTGCATAACAAGTATCA+AGG 0.550052 7_3:-61178900 Msa1114360:CDS
AACCTTCAAAGGAATATGGT+TGG 0.557337 7_3:-61178977 Msa1114360:CDS
AGTGCTTTGGACTGCATGCA+AGG 0.558385 7_3:-61178632 Msa1114360:three_prime_UTR
AATGGCCAGAATGATTATCA+TGG 0.560785 7_3:-61178951 Msa1114360:CDS
AGTAGGTCCCCATCCAGGAC+CGG 0.560984 7_3:+61179133 None:intergenic
AATAACCCCGGACATCATCA+TGG 0.580939 7_3:-61178801 Msa1114360:CDS
GATCAGTAATCATCATCAAG+TGG 0.601542 7_3:-61180964 Msa1114360:CDS
TGAGTGTCGGGATCAGGCAA+TGG 0.603390 7_3:+61179302 None:intergenic
CTTTCCTTTATAGGACGATG+AGG 0.606347 7_3:+61180929 None:intergenic
AATAGAGGAAGAAGAAGAAG+CGG 0.613668 7_3:-61178759 Msa1114360:CDS
ATCAGTAATCATCATCAAGT+GGG 0.617268 7_3:-61180963 Msa1114360:CDS
AGGTAGCTACTCCTGGTGCA+TGG 0.624734 7_3:-61178668 Msa1114360:CDS
GGATGTGGAAAGAGAAGCAG+AGG 0.624882 7_3:-61179231 Msa1114360:CDS
TCAGGCAATGGTATGTCGCA+GGG 0.628570 7_3:+61179314 None:intergenic
TGGCTCAGTTCCATGGCGAA+AGG 0.630820 7_3:-61179456 Msa1114360:CDS
GGATGACTCTGCTGTCAAAG+AGG 0.633767 7_3:-61179384 Msa1114360:CDS
AAGAGAACTACCAAGAATCA+CGG 0.642265 7_3:+61179175 None:intergenic
AATGGCGGTAATGGAAATTG+GGG 0.644690 7_3:-61178873 Msa1114360:CDS
AGAGAGAGAACAATATGTCG+TGG 0.659005 7_3:-61178827 Msa1114360:CDS
AAGTATCAAGGAAGAAATGG+CGG 0.663489 7_3:-61178888 Msa1114360:CDS
AGGACAGAGGAAGCGTTCCG+AGG 0.663665 7_3:+61181251 None:intergenic
ATGTCGTGGTCTAATAACCC+CGG 0.670337 7_3:-61178813 Msa1114360:CDS
TGTGGAAGCAAGAACAACCT+CGG 0.672529 7_3:-61181268 None:intergenic
AAATGGTACTAACCCAAAGG+TGG 0.674375 7_3:+61180906 None:intergenic
CCACATCCAGATAAAGTTCG+GGG 0.678042 7_3:+61179246 None:intergenic
TTATCATGGATGGAATCAGA+CGG 0.682338 7_3:-61178937 Msa1114360:CDS
TAGTGCCATGATGATGTCCG+GGG 0.697507 7_3:+61178796 None:intergenic
TATGTATCTGCATCCCAATG+TGG 0.712444 7_3:-61179414 Msa1114360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAATTAAGAAACTTT+GGG + chr7_3:61178556-61178575 None:intergenic 10.0%
!! AAAAAATAATTAAGAAACTT+TGG + chr7_3:61178557-61178576 None:intergenic 10.0%
!!! TTTTCTTTTAGTTTATTTTA+AGG - chr7_3:61178844-61178863 Msa1114360:CDS 10.0%
!!! TTTTCTTTTAATTACTGAAA+TGG - chr7_3:61178638-61178657 Msa1114360:three_prime_UTR 15.0%
!! TAACAAATAAAACTAAAGAA+AGG + chr7_3:61178782-61178801 None:intergenic 15.0%
!! ATTTAATTATAGAAATGTAC+AGG - chr7_3:61179427-61179446 Msa1114360:CDS 15.0%
!! TTTAATTATAGAAATGTACA+GGG - chr7_3:61179428-61179447 Msa1114360:CDS 15.0%
!! AAATTTAACCAAACTTAAAA+TGG + chr7_3:61179692-61179711 None:intergenic 15.0%
!!! TATCTACTAATTAAATCTTT+TGG - chr7_3:61179881-61179900 Msa1114360:intron 15.0%
!! ATAAACACTTATTTATTGAA+TGG + chr7_3:61180004-61180023 None:intergenic 15.0%
!!! AAAATCAATTGGTAATTTAA+GGG + chr7_3:61180097-61180116 None:intergenic 15.0%
!!! TAAAATCAATTGGTAATTTA+AGG + chr7_3:61180098-61180117 None:intergenic 15.0%
!! AAGGAATAAATAAAATCAAT+TGG + chr7_3:61180108-61180127 None:intergenic 15.0%
!!! TTGATTTTATTTATTCCTTT+TGG - chr7_3:61180109-61180128 Msa1114360:intron 15.0%
!! ATTATATCATATAAGAAGTA+TGG - chr7_3:61181111-61181130 Msa1114360:five_prime_UTR 15.0%
!! TAATTACTGAAATGGATAAT+TGG - chr7_3:61178646-61178665 Msa1114360:exon 20.0%
!!! GATCAGTGATTTAAAATAAT+TGG - chr7_3:61178893-61178912 Msa1114360:CDS 20.0%
!! ATTGAACATATTCAAAAGTA+GGG + chr7_3:61178991-61179010 None:intergenic 20.0%
!! AATTGAACATATTCAAAAGT+AGG + chr7_3:61178992-61179011 None:intergenic 20.0%
!!! TACTTTTGAATATGTTCAAT+TGG - chr7_3:61178991-61179010 Msa1114360:CDS 20.0%
!!! ACTTTTGAATATGTTCAATT+GGG - chr7_3:61178992-61179011 Msa1114360:CDS 20.0%
!! TGAAATGTTGTAAAATATGA+TGG - chr7_3:61179232-61179251 Msa1114360:CDS 20.0%
!! CAAGATATTCAATAATTGAA+GGG - chr7_3:61179292-61179311 Msa1114360:CDS 20.0%
!! TTCTATAATTAAATGACGTA+GGG + chr7_3:61179421-61179440 None:intergenic 20.0%
!! TTTCTATAATTAAATGACGT+AGG + chr7_3:61179422-61179441 None:intergenic 20.0%
!!! AAATGAAACCATTTTAAGTT+TGG - chr7_3:61179681-61179700 Msa1114360:intron 20.0%
!!! TTGTTTCATTATTTTCATGT+TGG + chr7_3:61180617-61180636 None:intergenic 20.0%
!! AAAATAATGAAACAAACTCT+TGG - chr7_3:61180622-61180641 Msa1114360:intron 20.0%
!! AAATAATGAAACAAACTCTT+GGG - chr7_3:61180623-61180642 Msa1114360:intron 20.0%
!!! AAATTTTTCTTATGTGGAAA+AGG - chr7_3:61180959-61180978 Msa1114360:CDS 20.0%
!!! ATTTTGTAAAATTCATCAGA+TGG - chr7_3:61181145-61181164 Msa1114360:five_prime_UTR 20.0%
! TTTCGGAGATAATTATATGA+AGG + chr7_3:61178492-61178511 None:intergenic 25.0%
!! AATTAAGAAACTTTGGGAAA+AGG + chr7_3:61178550-61178569 None:intergenic 25.0%
! ATAATTGGAGAAAAAACCAA+GGG - chr7_3:61178661-61178680 Msa1114360:exon 25.0%
!! CTTTTGAATATGTTCAATTG+GGG - chr7_3:61178993-61179012 Msa1114360:CDS 25.0%
!!! TGGTTTTGAATGAACAATTT+TGG - chr7_3:61179035-61179054 Msa1114360:CDS 25.0%
!! ATTTTTCTTAGGATGAGTTT+TGG - chr7_3:61179106-61179125 Msa1114360:CDS 25.0%
! AATGTTGTAAAATATGATGG+AGG - chr7_3:61179235-61179254 Msa1114360:CDS 25.0%
!! ATGTTGTAAAATATGATGGA+GGG - chr7_3:61179236-61179255 Msa1114360:CDS 25.0%
! CCTTCAATTATTGAATATCT+TGG + chr7_3:61179294-61179313 None:intergenic 25.0%
! CCAAGATATTCAATAATTGA+AGG - chr7_3:61179291-61179310 Msa1114360:CDS 25.0%
!! ATGAATTGTTGTCATTTCTT+TGG - chr7_3:61179330-61179349 Msa1114360:CDS 25.0%
! ACAAAAGATAGACTAAAGAA+AGG + chr7_3:61179661-61179680 None:intergenic 25.0%
!!! GGTTAAATTTGTTCTGAAAA+TGG - chr7_3:61179702-61179721 Msa1114360:intron 25.0%
!!! TCTGAAAATGGTTTTGATTT+TGG - chr7_3:61179714-61179733 Msa1114360:intron 25.0%
! ACAGGAAATTTAAGTATCTT+TGG + chr7_3:61179819-61179838 None:intergenic 25.0%
!! AAGAGTGTAGTTAAATAAAC+AGG + chr7_3:61179837-61179856 None:intergenic 25.0%
!! CTACTAATTAAATCTTTTGG+AGG - chr7_3:61179884-61179903 Msa1114360:intron 25.0%
!!! TTACTTTTCAAAGTATCCTT+AGG - chr7_3:61179912-61179931 Msa1114360:intron 25.0%
! TTCAATAATTGAATGACCTA+AGG + chr7_3:61179931-61179950 None:intergenic 25.0%
! AGGTCATTCAATTATTGAAT+AGG - chr7_3:61179932-61179951 Msa1114360:intron 25.0%
!!! TAGTGTATGAAATGTTTTGA+TGG - chr7_3:61181193-61181212 Msa1114360:five_prime_UTR 25.0%
ATGATGTTGGAACAAAAACA+AGG + chr7_3:61178419-61178438 None:intergenic 30.0%
AAAGAGAAAGACACAGATTT+CGG + chr7_3:61178509-61178528 None:intergenic 30.0%
! CTTTTTGAATTTCCTCATCT+GGG - chr7_3:61178612-61178631 Msa1114360:three_prime_UTR 30.0%
GATAATTGGAGAAAAAACCA+AGG - chr7_3:61178660-61178679 Msa1114360:exon 30.0%
ATCAGTAATCATCATCAAGT+GGG - chr7_3:61178684-61178703 Msa1114360:CDS 30.0%
ATTCAACTGCAACTCAATTA+TGG + chr7_3:61178941-61178960 None:intergenic 30.0%
! TAAAATATGATGGAGGGTTT+AGG - chr7_3:61179242-61179261 Msa1114360:CDS 30.0%
! AGGTTGTAATTTCTTGTTGT+TGG - chr7_3:61179366-61179385 Msa1114360:CDS 30.0%
! AACTATGTTTCTGTTTGTGT+TGG - chr7_3:61179460-61179479 Msa1114360:CDS 30.0%
GTCAATTTAATGATGAGGTA+GGG + chr7_3:61179615-61179634 None:intergenic 30.0%
ATTAAATTGACGAAGCTTAC+AGG - chr7_3:61179624-61179643 Msa1114360:intron 30.0%
!! ATGTTCAAGTTGGTATCTAT+TGG - chr7_3:61179739-61179758 Msa1114360:intron 30.0%
TCAATGATGACACACATTAT+CGG + chr7_3:61179780-61179799 None:intergenic 30.0%
TTCGTGTATTGAAAATGTCT+AGG - chr7_3:61179858-61179877 Msa1114360:intron 30.0%
! CTAGACAGATTTAGTTCAAA+AGG + chr7_3:61179975-61179994 None:intergenic 30.0%
!! AAGAAGTTTTGTGAATCGAT+TGG - chr7_3:61180171-61180190 Msa1114360:intron 30.0%
CATTTGACAATGCAAAAAAC+AGG - chr7_3:61180286-61180305 Msa1114360:intron 30.0%
! ACAATGCAAAAAACAGGTTT+TGG - chr7_3:61180292-61180311 Msa1114360:intron 30.0%
! CAATGCAAAAAACAGGTTTT+GGG - chr7_3:61180293-61180312 Msa1114360:intron 30.0%
AACAAGTATCAAGGAAGAAA+TGG - chr7_3:61180756-61180775 Msa1114360:intron 30.0%
AATGAATATCAGCTGAATAG+AGG - chr7_3:61180873-61180892 Msa1114360:intron 30.0%
TAAATGAAGATCAAGTGCTT+TGG - chr7_3:61181002-61181021 Msa1114360:exon 30.0%
AGTACTAATGTACTGAATCA+TGG + chr7_3:61181223-61181242 None:intergenic 30.0%
ATGATTCAGTACATTAGTAC+TGG - chr7_3:61181222-61181241 Msa1114360:five_prime_UTR 30.0%
! GATGATGATGATGATGATGT+TGG + chr7_3:61178432-61178451 None:intergenic 35.0%
ATATGAAGGTGATGAGAAGA+AGG + chr7_3:61178478-61178497 None:intergenic 35.0%
! GCTTTTTGAATTTCCTCATC+TGG - chr7_3:61178611-61178630 Msa1114360:three_prime_UTR 35.0%
AAAAGAAAAAGACCCAGATG+AGG + chr7_3:61178627-61178646 None:intergenic 35.0%
GATCAGTAATCATCATCAAG+TGG - chr7_3:61178683-61178702 Msa1114360:CDS 35.0%
AAATCCTCATCGTCCTATAA+AGG - chr7_3:61178714-61178733 Msa1114360:CDS 35.0%
TATAAAGGAAAGCCACCTTT+GGG - chr7_3:61178729-61178748 Msa1114360:CDS 35.0%
AGAAAATGGTACTAACCCAA+AGG + chr7_3:61178747-61178766 None:intergenic 35.0%
AATATGTTCAATTGGGGTGT+TGG - chr7_3:61178999-61179018 Msa1114360:CDS 35.0%
!! TGGGGTGTTGGTTTAAAAAT+TGG - chr7_3:61179011-61179030 Msa1114360:CDS 35.0%
!! GTGTTGGTTTAAAAATTGGC+TGG - chr7_3:61179015-61179034 Msa1114360:CDS 35.0%
!!! CAATTTTGGTTTTGTGCTAG+TGG - chr7_3:61179049-61179068 Msa1114360:CDS 35.0%
!!! TAGGATGAGTTTTGGTTTGA+TGG - chr7_3:61179114-61179133 Msa1114360:CDS 35.0%
!!! CTTGGTCATTTTTGTTGACA+AGG + chr7_3:61179276-61179295 None:intergenic 35.0%
AATTGAAGGGAGTTTCACTA+AGG - chr7_3:61179305-61179324 Msa1114360:CDS 35.0%
! ATTGTTGTCATTTCTTTGGC+TGG - chr7_3:61179334-61179353 Msa1114360:CDS 35.0%
CAATGTGTCACATCAAGATT+CGG + chr7_3:61179584-61179603 None:intergenic 35.0%
CGTCAATTTAATGATGAGGT+AGG + chr7_3:61179616-61179635 None:intergenic 35.0%
GCTTCGTCAATTTAATGATG+AGG + chr7_3:61179620-61179639 None:intergenic 35.0%
!! GATTTTGGCAATGTTCAAGT+TGG - chr7_3:61179729-61179748 Msa1114360:intron 35.0%
AATGATGACACACATTATCG+GGG + chr7_3:61179778-61179797 None:intergenic 35.0%
CAATGATGACACACATTATC+GGG + chr7_3:61179779-61179798 None:intergenic 35.0%
! CTTTTGCATGTTTTCCTTCT+AGG - chr7_3:61180055-61180074 Msa1114360:intron 35.0%
TTCCTTCTAGGTGTGATTAT+AGG - chr7_3:61180067-61180086 Msa1114360:intron 35.0%
AACCTATAATCACACCTAGA+AGG + chr7_3:61180072-61180091 None:intergenic 35.0%
! CAAAACTTCTTTTCCCACAA+AGG + chr7_3:61180162-61180181 None:intergenic 35.0%
TCATCCCAATTTACCACATT+GGG + chr7_3:61180249-61180268 None:intergenic 35.0%
TACATCATCAATGTGAGTGT+CGG + chr7_3:61180361-61180380 None:intergenic 35.0%
!! CTCACATTGATGATGTAGAT+TGG - chr7_3:61180364-61180383 Msa1114360:intron 35.0%
TTCCACATCCAGATAAAGTT+CGG + chr7_3:61180406-61180425 None:intergenic 35.0%
AAGAGAACTACCAAGAATCA+CGG + chr7_3:61180475-61180494 None:intergenic 35.0%
GTTGAACCTTCAAAGGAATA+TGG - chr7_3:61180666-61180685 Msa1114360:intron 35.0%
AACCTTCAAAGGAATATGGT+TGG - chr7_3:61180670-61180689 Msa1114360:intron 35.0%
AATGGCCAGAATGATTATCA+TGG - chr7_3:61180696-61180715 Msa1114360:intron 35.0%
TCCATCCATGATAATCATTC+TGG + chr7_3:61180704-61180723 None:intergenic 35.0%
TTATCATGGATGGAATCAGA+CGG - chr7_3:61180710-61180729 Msa1114360:intron 35.0%
GACTTGCATAACAAGTATCA+AGG - chr7_3:61180747-61180766 Msa1114360:intron 35.0%
AAGTATCAAGGAAGAAATGG+CGG - chr7_3:61180759-61180778 Msa1114360:intron 35.0%
!! AAATGGCGGTAATGGAAATT+GGG - chr7_3:61180773-61180792 Msa1114360:intron 35.0%
AATAGAGGAAGAAGAAGAAG+CGG - chr7_3:61180888-61180907 Msa1114360:intron 35.0%
! GAGGGGAAATTTTTCTTATG+TGG - chr7_3:61180953-61180972 Msa1114360:CDS 35.0%
!! CAGATGGCTTTATTGTCTAT+TGG - chr7_3:61181161-61181180 Msa1114360:five_prime_UTR 35.0%
TGGAACAAAAACAAGGACAG+AGG + chr7_3:61178412-61178431 None:intergenic 40.0%
GATGATGATTACTGATCCCT+TGG + chr7_3:61178680-61178699 None:intergenic 40.0%
CTTTCCTTTATAGGACGATG+AGG + chr7_3:61178721-61178740 None:intergenic 40.0%
CAAAGGTGGCTTTCCTTTAT+AGG + chr7_3:61178730-61178749 None:intergenic 40.0%
CTATAAAGGAAAGCCACCTT+TGG - chr7_3:61178728-61178747 Msa1114360:CDS 40.0%
AAATGGTACTAACCCAAAGG+TGG + chr7_3:61178744-61178763 None:intergenic 40.0%
GGAAAGAGAGAGAGAGAAAA+TGG + chr7_3:61178761-61178780 None:intergenic 40.0%
!!! TTTGGTTTTGTGCTAGTGGT+AGG - chr7_3:61179053-61179072 Msa1114360:CDS 40.0%
TAAGCCTCTTCTCACAAAAG+AGG + chr7_3:61179159-61179178 None:intergenic 40.0%
! TTGTCATTTCTTTGGCTGGT+GGG - chr7_3:61179338-61179357 Msa1114360:CDS 40.0%
! TGGTGTTTTGCATGATCATC+AGG - chr7_3:61179386-61179405 Msa1114360:CDS 40.0%
! GTTTTGCATGATCATCAGGT+TGG - chr7_3:61179390-61179409 Msa1114360:CDS 40.0%
! CTTTGATTTCATGTCGTCTG+TGG - chr7_3:61179528-61179547 Msa1114360:intron 40.0%
ATGATGACACACATTATCGG+GGG + chr7_3:61179777-61179796 None:intergenic 40.0%
CCTATTATCTGCGAACCAAA+AGG + chr7_3:61180127-61180146 None:intergenic 40.0%
!!! CCTTTTGGTTCGCAGATAAT+AGG - chr7_3:61180124-61180143 Msa1114360:intron 40.0%
TATGTATCTGCATCCCAATG+TGG - chr7_3:61180233-61180252 Msa1114360:intron 40.0%
TGCATCCCAATGTGGTAAAT+TGG - chr7_3:61180241-61180260 Msa1114360:intron 40.0%
GCATCCCAATGTGGTAAATT+GGG - chr7_3:61180242-61180261 Msa1114360:intron 40.0%
GTCATCCCAATTTACCACAT+TGG + chr7_3:61180250-61180269 None:intergenic 40.0%
ACATCATCAATGTGAGTGTC+GGG + chr7_3:61180360-61180379 None:intergenic 40.0%
TCCACATCCAGATAAAGTTC+GGG + chr7_3:61180405-61180424 None:intergenic 40.0%
! CTCTTGAATCAGTCCTTTTC+CGG - chr7_3:61180495-61180514 Msa1114360:intron 40.0%
TGAACAGAATTATGCTGCTC+AGG - chr7_3:61180578-61180597 Msa1114360:intron 40.0%
AGAATTATGCTGCTCAGGAA+TGG - chr7_3:61180583-61180602 Msa1114360:intron 40.0%
GAATTATGCTGCTCAGGAAT+GGG - chr7_3:61180584-61180603 Msa1114360:intron 40.0%
TCCTGTTGTTGAACCTTCAA+AGG - chr7_3:61180659-61180678 Msa1114360:intron 40.0%
! TCCTTTGAAGGTTCAACAAC+AGG + chr7_3:61180663-61180682 None:intergenic 40.0%
AAGGAATATGGTTGGCGAAA+TGG - chr7_3:61180678-61180697 Msa1114360:intron 40.0%
GCCAGAATGATTATCATGGA+TGG - chr7_3:61180700-61180719 Msa1114360:intron 40.0%
!! GAAATGGCGGTAATGGAAAT+TGG - chr7_3:61180772-61180791 Msa1114360:intron 40.0%
!! AATGGCGGTAATGGAAATTG+GGG - chr7_3:61180774-61180793 Msa1114360:intron 40.0%
! TAATGGAAATTGGGGAGCAT+GGG - chr7_3:61180782-61180801 Msa1114360:intron 40.0%
AGAGAGAGAACAATATGTCG+TGG - chr7_3:61180820-61180839 Msa1114360:intron 40.0%
! ATTAGTGCCATGATGATGTC+CGG + chr7_3:61180856-61180875 None:intergenic 40.0%
ATCTTCATTTACCATGCACC+AGG + chr7_3:61180993-61181012 None:intergenic 40.0%
ACTCCTATGAAGCTGCTAAA+GGG - chr7_3:61181038-61181057 Msa1114360:five_prime_UTR 40.0%
!! CAGTTTTGTGAAAGTAGCTG+AGG - chr7_3:61181062-61181081 Msa1114360:five_prime_UTR 40.0%
ATCATCATCAAGTGGGTCAC+TGG - chr7_3:61178691-61178710 Msa1114360:CDS 45.0%
! CATGCCTCTTTTGTGAGAAG+AGG - chr7_3:61179152-61179171 Msa1114360:CDS 45.0%
TGTGTGTGACTCATGTGACA+GGG - chr7_3:61179206-61179225 Msa1114360:CDS 45.0%
! GTTGTCATTTCTTTGGCTGG+TGG - chr7_3:61179337-61179356 Msa1114360:CDS 45.0%
!! TGCATGATCATCAGGTTGGT+TGG - chr7_3:61179394-61179413 Msa1114360:CDS 45.0%
GTTTCCAACAGTGCCTTTGT+GGG - chr7_3:61180146-61180165 Msa1114360:intron 45.0%
!!! TTTTCCCACAAAGGCACTGT+TGG + chr7_3:61180153-61180172 None:intergenic 45.0%
! AATCGATTGGCTCAGTTCCA+TGG - chr7_3:61180184-61180203 Msa1114360:intron 45.0%
CCTTCTATTACCTTTCGCCA+TGG + chr7_3:61180204-61180223 None:intergenic 45.0%
CCATGGCGAAAGGTAATAGA+AGG - chr7_3:61180201-61180220 Msa1114360:intron 45.0%
!!! AGGTTTTGGGCTGAGATCAA+CGG - chr7_3:61180306-61180325 Msa1114360:intron 45.0%
CCACATCCAGATAAAGTTCG+GGG + chr7_3:61180404-61180423 None:intergenic 45.0%
! TTTTTCTTCCATGTTGCGGC+GGG + chr7_3:61180442-61180461 None:intergenic 45.0%
! CTTTTTCTTCCATGTTGCGG+CGG + chr7_3:61180443-61180462 None:intergenic 45.0%
! CGCCTTTTTCTTCCATGTTG+CGG + chr7_3:61180446-61180465 None:intergenic 45.0%
CAACATGGAAGAAAAAGGCG+AGG - chr7_3:61180446-61180465 Msa1114360:intron 45.0%
! TCAACAACAGGAGCACGATA+TGG + chr7_3:61180651-61180670 None:intergenic 45.0%
CGCCAACCATATTCCTTTGA+AGG + chr7_3:61180675-61180694 None:intergenic 45.0%
TGGAATCAGACGGAGCAATA+TGG - chr7_3:61180720-61180739 Msa1114360:intron 45.0%
CAAGGAAGAAATGGCGGTAA+TGG - chr7_3:61180765-61180784 Msa1114360:intron 45.0%
! GTAATGGAAATTGGGGAGCA+TGG - chr7_3:61180781-61180800 Msa1114360:intron 45.0%
ATGTCGTGGTCTAATAACCC+CGG - chr7_3:61180834-61180853 Msa1114360:intron 45.0%
AATAACCCCGGACATCATCA+TGG - chr7_3:61180846-61180865 Msa1114360:intron 45.0%
! TTAGTGCCATGATGATGTCC+GGG + chr7_3:61180855-61180874 None:intergenic 45.0%
GACTCCTATGAAGCTGCTAA+AGG - chr7_3:61181037-61181056 Msa1114360:five_prime_UTR 45.0%
TGACCCTTTAGCAGCTTCAT+AGG + chr7_3:61181044-61181063 None:intergenic 45.0%
!!! ATTTTAATTTAATTTTTCTT+AGG - chr7_3:61179095-61179114 Msa1114360:CDS 5.0%
GTGTGTGTGACTCATGTGAC+AGG - chr7_3:61179205-61179224 Msa1114360:CDS 50.0%
!! TCTTTGGCTGGTGGGTTGTT+AGG - chr7_3:61179346-61179365 Msa1114360:CDS 50.0%
GGTTTCCAACAGTGCCTTTG+TGG - chr7_3:61180145-61180164 Msa1114360:intron 50.0%
GGATGACTCTGCTGTCAAAG+AGG - chr7_3:61180263-61180282 Msa1114360:intron 50.0%
TCAGGCAATGGTATGTCGCA+GGG + chr7_3:61180336-61180355 None:intergenic 50.0%
ATCAGGCAATGGTATGTCGC+AGG + chr7_3:61180337-61180356 None:intergenic 50.0%
!! TCAATGTGAGTGTCGGGATC+AGG + chr7_3:61180354-61180373 None:intergenic 50.0%
TGTCGACCCCGAACTTTATC+TGG - chr7_3:61180395-61180414 Msa1114360:intron 50.0%
CCCCGAACTTTATCTGGATG+TGG - chr7_3:61180401-61180420 Msa1114360:intron 50.0%
GGATGTGGAAAGAGAAGCAG+AGG - chr7_3:61180416-61180435 Msa1114360:intron 50.0%
CGCCGCAACATGGAAGAAAA+AGG - chr7_3:61180441-61180460 Msa1114360:intron 50.0%
! AATCAGTCCTTTTCCGGTCC+TGG - chr7_3:61180501-61180520 Msa1114360:intron 50.0%
! TAGTGCCATGATGATGTCCG+GGG + chr7_3:61180854-61180873 None:intergenic 50.0%
AGAGGAAGAAGAAGAAGCGG+AGG - chr7_3:61180891-61180910 Msa1114360:intron 50.0%
AGAAGAAGAAGCGGAGGAAG+AGG - chr7_3:61180897-61180916 Msa1114360:intron 50.0%
GTGGAAAAGGTAGCTACTCC+TGG - chr7_3:61180972-61180991 Msa1114360:CDS 50.0%
! AGTGCTTTGGACTGCATGCA+AGG - chr7_3:61181015-61181034 Msa1114360:five_prime_UTR 50.0%
TGGCTCAGTTCCATGGCGAA+AGG - chr7_3:61180191-61180210 Msa1114360:intron 55.0%
!! TGAGTGTCGGGATCAGGCAA+TGG + chr7_3:61180348-61180367 None:intergenic 55.0%
! AGTCCTTTTCCGGTCCTGGA+TGG - chr7_3:61180505-61180524 Msa1114360:intron 55.0%
! GTCCTTTTCCGGTCCTGGAT+GGG - chr7_3:61180506-61180525 Msa1114360:intron 55.0%
! TCCTTTTCCGGTCCTGGATG+GGG - chr7_3:61180507-61180526 Msa1114360:intron 55.0%
TGGATGGGGACCTACTGGAT+GGG - chr7_3:61180521-61180540 Msa1114360:intron 55.0%
! GGAAATTGGGGAGCATGGGA+TGG - chr7_3:61180786-61180805 Msa1114360:intron 55.0%
AGAAGAAGCGGAGGAAGAGG+AGG - chr7_3:61180900-61180919 Msa1114360:intron 55.0%
AGGTAGCTACTCCTGGTGCA+TGG - chr7_3:61180979-61180998 Msa1114360:CDS 55.0%
!! AGGACAGAGGAAGCGTTCCG+AGG + chr7_3:61178399-61178418 None:intergenic 60.0%
TCCCCATCCAGGACCGGAAA+AGG + chr7_3:61180511-61180530 None:intergenic 60.0%
AGTAGGTCCCCATCCAGGAC+CGG + chr7_3:61180517-61180536 None:intergenic 60.0%
CATCCAGTAGGTCCCCATCC+AGG + chr7_3:61180522-61180541 None:intergenic 60.0%
CTGGATGGGGACCTACTGGA+TGG - chr7_3:61180520-61180539 Msa1114360:intron 60.0%
GGATGGGGACCTACTGGATG+GGG - chr7_3:61180522-61180541 Msa1114360:intron 60.0%
GATGGGGACCTACTGGATGG+GGG - chr7_3:61180523-61180542 Msa1114360:intron 60.0%
ATGGGGACCTACTGGATGGG+GGG - chr7_3:61180524-61180543 Msa1114360:intron 60.0%
TCTTCGTCCCCCCATCCAGT+AGG + chr7_3:61180534-61180553 None:intergenic 60.0%
AGAAGCGGAGGAAGAGGAGG+AGG - chr7_3:61180903-61180922 Msa1114360:intron 60.0%
AGAGGAGGAGGAGGAGGAAG+AGG - chr7_3:61180915-61180934 Msa1114360:intron 60.0%
GAAGAGGTGGAGGAGGAGGA+AGG - chr7_3:61180931-61180950 Msa1114360:CDS 60.0%
AGGTGGAGGAGGAGGAAGGA+GGG - chr7_3:61180935-61180954 Msa1114360:CDS 60.0%
GGCGAGGCAGCCGTGATTCT+TGG - chr7_3:61180462-61180481 Msa1114360:intron 65.0%
GGTCCTGGATGGGGACCTAC+TGG - chr7_3:61180516-61180535 Msa1114360:intron 65.0%
AGCGGAGGAAGAGGAGGAGG+AGG - chr7_3:61180906-61180925 Msa1114360:intron 65.0%
GGAGGAAGAGGAGGAGGAGG+AGG - chr7_3:61180909-61180928 Msa1114360:intron 65.0%
GGAGGAGGAGGAGGAAGAGG+TGG - chr7_3:61180918-61180937 Msa1114360:intron 65.0%
GGAGGAGGAGGAAGAGGTGG+AGG - chr7_3:61180921-61180940 Msa1114360:CDS 65.0%
GGAGGAGGAAGAGGTGGAGG+AGG - chr7_3:61180924-61180943 Msa1114360:CDS 65.0%
GGAGGAAGAGGTGGAGGAGG+AGG - chr7_3:61180927-61180946 Msa1114360:CDS 65.0%
GAGGTGGAGGAGGAGGAAGG+AGG - chr7_3:61180934-61180953 Msa1114360:CDS 65.0%
GGTGGAGGAGGAGGAAGGAG+GGG - chr7_3:61180936-61180955 Msa1114360:CDS 65.0%
AGCAGAGGCCCGCCGCAACA+TGG - chr7_3:61180431-61180450 Msa1114360:intron 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 61178390 61181279 61178390 ID=Msa1114360;Name=Msa1114360
chr7_3 mRNA 61178390 61181279 61178390 ID=Msa1114360-mRNA-1;Parent=Msa1114360;Name=Msa1114360-mRNA-1;_AED=0.01;_eAED=0.01;_QI=267|1|1|1|1|1|2|275|319
chr7_3 exon 61178390 61179530 61178390 ID=Msa1114360-mRNA-1:exon:13817;Parent=Msa1114360-mRNA-1
chr7_3 exon 61180919 61181279 61180919 ID=Msa1114360-mRNA-1:exon:13816;Parent=Msa1114360-mRNA-1
chr7_3 five_prime_UTR 61181013 61181279 61181013 ID=Msa1114360-mRNA-1:five_prime_utr;Parent=Msa1114360-mRNA-1
chr7_3 CDS 61180919 61181012 61180919 ID=Msa1114360-mRNA-1:cds;Parent=Msa1114360-mRNA-1
chr7_3 CDS 61178665 61179530 61178665 ID=Msa1114360-mRNA-1:cds;Parent=Msa1114360-mRNA-1
chr7_3 three_prime_UTR 61178390 61178664 61178390 ID=Msa1114360-mRNA-1:three_prime_utr;Parent=Msa1114360-mRNA-1
Gene Sequence

>Msa1114360

ATGGATAATTGGAGAAAAAACCAAGGGATCAGTAATCATCATCAAGTGGGTCACTGGAAATCCTCATCGTCCTATAAAGGAAAGCCACCTTTGGATAATAGGTTTCCAACAGTGCCTTTGTGGGAAAAGAAGTTTTGTGAATCGATTGGCTCAGTTCCATGGCGAAAGGTAATAGAAGGCAATAAGTATATGTATCTGCATCCCAATGTGGTAAATTGGGATGACTCTGCTGTCAAAGAGGCATTTGACAATGCAAAAAACAGGTTTTGGGCTGAGATCAACGGCATTCCCTGCGACATACCATTGCCTGATCCCGACACTCACATTGATGATGTAGATTGGAATGCAAGTGTCGACCCCGAACTTTATCTGGATGTGGAAAGAGAAGCAGAGGCCCGCCGCAACATGGAAGAAAAAGGCGAGGCAGCCGTGATTCTTGGTAGTTCTCTTCTCTTGAATCAGTCCTTTTCCGGTCCTGGATGGGGACCTACTGGATGGGGGGACGAAGAAGAAAAAGAAGTAACAAAGACTTCTGAACAGAATTATGCTGCTCAGGAATGGGAATTGAACCAACATGAAAATAATGAAACAAACTCTTGGGAACCATATCGTGCTCCTGTTGTTGAACCTTCAAAGGAATATGGTTGGCGAAATGGCCAGAATGATTATCATGGATGGAATCAGACGGAGCAATATGGTGCTGACTTGCATAACAAGTATCAAGGAAGAAATGGCGGTAATGGAAATTGGGGAGCATGGGATGGATATAACAGAAAGAGAGAGAACAATATGTCGTGGTCTAATAACCCCGGACATCATCATGGCACTAATGAATATCAGCTGAATAGAGGAAGAAGAAGAAGCGGAGGAAGAGGAGGAGGAGGAGGAAGAGGTGGAGGAGGAGGAAGGAGGGGAAATTTTTCTTATGTGGAAAAGGTAGCTACTCCTGGTGCATGGTAA
Protein sequence

>Msa1114360

MDNWRKNQGISNHHQVGHWKSSSSYKGKPPLDNRFPTVPLWEKKFCESIGSVPWRKVIEGNKYMYLHPNVVNWDDSAVKEAFDNAKNRFWAEINGIPCDIPLPDPDTHIDDVDWNASVDPELYLDVEREAEARRNMEEKGEAAVILGSSLLLNQSFSGPGWGPTGWGDEEEKEVTKTSEQNYAAQEWELNQHENNETNSWEPYRAPVVEPSKEYGWRNGQNDYHGWNQTEQYGADLHNKYQGRNGGNGNWGAWDGYNRKRENNMSWSNNPGHHHGTNEYQLNRGRRRSGGRGGGGGRGGGGGRRGNFSYVEKVATPGAW*