AlfalfaGEDB | Msa1114360

M. sativa cultivar zhongmu-4 / Msa1114360

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1114360	XP_003625087.2	93.417	319	16	2	1	319	1	314	0.0	534

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1114360	G7KSK5	93.417	319	16	2	1	319	1	314	0.0	534

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0014800	Msa1114360	0.807641	4.576312e-50	-8.615850e-47
Msa0449760	Msa1114360	0.811550	6.619104e-51	-8.615850e-47
Msa0610590	Msa1114360	0.801006	1.103418e-48	-8.615850e-47
Msa0835730	Msa1114360	0.803927	2.759366e-49	-8.615850e-47
Msa0891600	Msa1114360	-0.816919	4.313667e-52	-8.615850e-47
Msa0910980	Msa1114360	-0.812241	4.681124e-51	-8.615850e-47
Msa0927670	Msa1114360	0.808272	3.360529e-50	-8.615850e-47
Msa0992490	Msa1114360	-0.801984	6.955192e-49	-8.615850e-47
Msa1026760	Msa1114360	0.800948	1.134246e-48	-8.615850e-47
Msa1026790	Msa1114360	0.801790	7.623402e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1114360	MtrunA17_Chr7g0256981	93.417	319	16	2	1	319	1	314	0.0	534
Msa1114360	MtrunA17_Chr7g0257031	75.938	320	66	4	1	319	1	310	1.59e-144	409
Msa1114360	MtrunA17_Chr7g0257011	68.652	319	67	6	1	319	1	286	7.88e-130	371
Msa1114360	MtrunA17_Chr7g0256971	72.800	250	61	3	34	281	3	247	1.26e-120	347
Msa1114360	MtrunA17_Chr1g0172581	44.751	181	79	6	64	233	1	171	5.02e-39	136
Msa1114360	MtrunA17_Chr3g0085991	42.623	122	69	1	22	142	21	142	3.11e-31	120

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1114360	AT5G03990.1	34.839	310	155	8	1	305	1	268	4.39e-39	140
Msa1114360	AT3G51940.1	47.619	105	54	1	25	128	20	124	1.18e-30	120
Msa1114360	AT3G51940.2	52.174	92	43	1	38	128	37	128	2.74e-30	119
Msa1114360	AT5G03990.2	32.645	242	123	6	64	305	1	202	9.81e-22	92.4

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Find 115 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAATGAAACAAACTCT+TGG	0.438543	7_3:-61179025	Msa1114360:CDS
AAAGAGAAAGACACAGATTT+CGG	0.387442	7_3:+61181141	None:intergenic
AAATAATGAAACAAACTCTT+GGG	0.477297	7_3:-61179024	Msa1114360:CDS
AAATCCTCATCGTCCTATAA+AGG	0.372642	7_3:-61180933	Msa1114360:CDS
AAATGGCGGTAATGGAAATT+GGG	0.360900	7_3:-61178874	Msa1114360:CDS
AAATGGTACTAACCCAAAGG+TGG	0.674375	7_3:+61180906	None:intergenic
AACAAGTATCAAGGAAGAAA+TGG	0.421942	7_3:-61178891	Msa1114360:CDS
AACCTTCAAAGGAATATGGT+TGG	0.557337	7_3:-61178977	Msa1114360:CDS
AAGAGAACTACCAAGAATCA+CGG	0.642265	7_3:+61179175	None:intergenic
AAGGAATATGGTTGGCGAAA+TGG	0.391675	7_3:-61178969	Msa1114360:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGATGTTGGAACAAAAACA+AGG	+	chr7_3:61178419-61178438	None:intergenic	30.0%
AAAGAGAAAGACACAGATTT+CGG	+	chr7_3:61178509-61178528	None:intergenic	30.0%
GATAATTGGAGAAAAAACCA+AGG	-	chr7_3:61178660-61178679	Msa1114360:exon	30.0%
ATCAGTAATCATCATCAAGT+GGG	-	chr7_3:61178684-61178703	Msa1114360:CDS	30.0%
ATTCAACTGCAACTCAATTA+TGG	+	chr7_3:61178941-61178960	None:intergenic	30.0%
GTCAATTTAATGATGAGGTA+GGG	+	chr7_3:61179615-61179634	None:intergenic	30.0%
ATTAAATTGACGAAGCTTAC+AGG	-	chr7_3:61179624-61179643	Msa1114360:intron	30.0%
TCAATGATGACACACATTAT+CGG	+	chr7_3:61179780-61179799	None:intergenic	30.0%
TTCGTGTATTGAAAATGTCT+AGG	-	chr7_3:61179858-61179877	Msa1114360:intron	30.0%
CATTTGACAATGCAAAAAAC+AGG	-	chr7_3:61180286-61180305	Msa1114360:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	61178390	61181279	61178390	ID=Msa1114360;Name=Msa1114360
chr7_3	mRNA	61178390	61181279	61178390	ID=Msa1114360-mRNA-1;Parent=Msa1114360;Name=Msa1114360-mRNA-1;_AED=0.01;_eAED=0.01;_QI=267\|1\|1\|1\|1\|1\|2\|275\|319
chr7_3	exon	61178390	61179530	61178390	ID=Msa1114360-mRNA-1:exon:13817;Parent=Msa1114360-mRNA-1
chr7_3	exon	61180919	61181279	61180919	ID=Msa1114360-mRNA-1:exon:13816;Parent=Msa1114360-mRNA-1
chr7_3	five_prime_UTR	61181013	61181279	61181013	ID=Msa1114360-mRNA-1:five_prime_utr;Parent=Msa1114360-mRNA-1
chr7_3	CDS	61180919	61181012	61180919	ID=Msa1114360-mRNA-1:cds;Parent=Msa1114360-mRNA-1
chr7_3	CDS	61178665	61179530	61178665	ID=Msa1114360-mRNA-1:cds;Parent=Msa1114360-mRNA-1
chr7_3	three_prime_UTR	61178390	61178664	61178390	ID=Msa1114360-mRNA-1:three_prime_utr;Parent=Msa1114360-mRNA-1

Gene Sequence

>Msa1114360

ATGGATAATTGGAGAAAAAACCAAGGGATCAGTAATCATCATCAAGTGGGTCACTGGAAATCCTCATCGTCCTATAAAGGAAAGCCACCTTTGGATAATAGGTTTCCAACAGTGCCTTTGTGGGAAAAGAAGTTTTGTGAATCGATTGGCTCAGTTCCATGGCGAAAGGTAATAGAAGGCAATAAGTATATGTATCTGCATCCCAATGTGGTAAATTGGGATGACTCTGCTGTCAAAGAGGCATTTGACAATGCAAAAAACAGGTTTTGGGCTGAGATCAACGGCATTCCCTGCGACATACCATTGCCTGATCCCGACACTCACATTGATGATGTAGATTGGAATGCAAGTGTCGACCCCGAACTTTATCTGGATGTGGAAAGAGAAGCAGAGGCCCGCCGCAACATGGAAGAAAAAGGCGAGGCAGCCGTGATTCTTGGTAGTTCTCTTCTCTTGAATCAGTCCTTTTCCGGTCCTGGATGGGGACCTACTGGATGGGGGGACGAAGAAGAAAAAGAAGTAACAAAGACTTCTGAACAGAATTATGCTGCTCAGGAATGGGAATTGAACCAACATGAAAATAATGAAACAAACTCTTGGGAACCATATCGTGCTCCTGTTGTTGAACCTTCAAAGGAATATGGTTGGCGAAATGGCCAGAATGATTATCATGGATGGAATCAGACGGAGCAATATGGTGCTGACTTGCATAACAAGTATCAAGGAAGAAATGGCGGTAATGGAAATTGGGGAGCATGGGATGGATATAACAGAAAGAGAGAGAACAATATGTCGTGGTCTAATAACCCCGGACATCATCATGGCACTAATGAATATCAGCTGAATAGAGGAAGAAGAAGAAGCGGAGGAAGAGGAGGAGGAGGAGGAAGAGGTGGAGGAGGAGGAAGGAGGGGAAATTTTTCTTATGTGGAAAAGGTAGCTACTCCTGGTGCATGGTAA

Protein sequence

>Msa1114360

MDNWRKNQGISNHHQVGHWKSSSSYKGKPPLDNRFPTVPLWEKKFCESIGSVPWRKVIEGNKYMYLHPNVVNWDDSAVKEAFDNAKNRFWAEINGIPCDIPLPDPDTHIDDVDWNASVDPELYLDVEREAEARRNMEEKGEAAVILGSSLLLNQSFSGPGWGPTGWGDEEEKEVTKTSEQNYAAQEWELNQHENNETNSWEPYRAPVVEPSKEYGWRNGQNDYHGWNQTEQYGADLHNKYQGRNGGNGNWGAWDGYNRKRENNMSWSNNPGHHHGTNEYQLNRGRRRSGGRGGGGGRGGGGGRRGNFSYVEKVATPGAW*