Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142570 | XP_013447886.1 | 94.161 | 137 | 8 | 0 | 1 | 137 | 1 | 137 | 3.96e-89 | 265 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142570 | A0A072TYJ6 | 94.161 | 137 | 8 | 0 | 1 | 137 | 1 | 137 | 1.89e-89 | 265 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1053310 | Msa1142570 | 0.834779 | 2.460505e-56 | -8.615850e-47 |
| Msa1053340 | Msa1142570 | 0.839786 | 1.286633e-57 | -8.615850e-47 |
| Msa1095410 | Msa1142570 | 0.805769 | 1.137552e-49 | -8.615850e-47 |
| Msa1142570 | Msa1142580 | 0.820042 | 8.451714e-53 | -8.615850e-47 |
| Msa1142570 | Msa1142590 | 0.827982 | 1.158157e-54 | -8.615850e-47 |
| Msa1142570 | Msa1142600 | 0.823420 | 1.398598e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142570 | MtrunA17_Chr7g0223391 | 94.161 | 137 | 8 | 0 | 1 | 137 | 18 | 154 | 8.35e-93 | 265 |
| Msa1142570 | MtrunA17_Chr7g0223401 | 91.241 | 137 | 12 | 0 | 1 | 137 | 6 | 142 | 1.77e-89 | 256 |
| Msa1142570 | MtrunA17_Chr8g0356181 | 48.571 | 140 | 69 | 1 | 1 | 137 | 1 | 140 | 1.53e-40 | 132 |
| Msa1142570 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 1.71e-19 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142570 | AT2G44310.1 | 48.175 | 137 | 69 | 1 | 1 | 135 | 1 | 137 | 3.64e-40 | 131 |
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_4:-17090964 | None:intergenic |
| CTTGAATGGCATAATTGATT+TGG | 0.284664 | 7_4:-17090709 | Msa1142570:CDS |
| TAAACACGAGCAAGCTCATT+TGG | 0.308764 | 7_4:+17090762 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_4:+17090919 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_4:-17090628 | Msa1142570:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_4:+17090624 | None:intergenic |
| GAAGGAGCTCAAGAGCTTGC+GGG | 0.426982 | 7_4:-17090823 | Msa1142570:CDS |
| AAACACGAGCAAGCTCATTT+GGG | 0.439893 | 7_4:+17090763 | None:intergenic |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.444846 | 7_4:-17090899 | Msa1142570:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_4:+17090925 | None:intergenic |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_4:+17090839 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_4:-17090634 | Msa1142570:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.477280 | 7_4:-17090723 | Msa1142570:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.506798 | 7_4:-17090841 | Msa1142570:CDS |
| ATTATGCTTGCAATGGCAGA+TGG | 0.516462 | 7_4:-17090660 | Msa1142570:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.518450 | 7_4:-17090655 | Msa1142570:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7_4:-17090906 | Msa1142570:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7_4:-17090910 | Msa1142570:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7_4:-17090577 | Msa1142570:CDS |
| CGGCTCGACACAAATAAAGA+TGG | 0.561664 | 7_4:-17090870 | Msa1142570:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.563155 | 7_4:-17090654 | Msa1142570:CDS |
| TGAAGGAGCTCAAGAGCTTG+CGG | 0.581055 | 7_4:-17090824 | Msa1142570:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_4:-17090793 | Msa1142570:CDS |
| CGGTAGGAAATCGGTTTGCT+CGG | 0.591236 | 7_4:-17090890 | Msa1142570:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_4:+17090935 | None:intergenic |
| TAAGCAGATTATGCTTGCAA+TGG | 0.607418 | 7_4:-17090667 | Msa1142570:CDS |
| TTATGCTTGCAATGGCAGAT+GGG | 0.653752 | 7_4:-17090659 | Msa1142570:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_4:17090569-17090588 | Msa1142570:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_4:17090613-17090632 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+CGG | - | chr7_4:17090619-17090638 | Msa1142570:CDS | 30.0% |
| CTTGAATGGCATAATTGATT+TGG | - | chr7_4:17090820-17090839 | Msa1142570:CDS | 30.0% | |
| ! | TGAAAGAGAGTACAATAAAG+TGG | - | chr7_4:17090952-17090971 | Msa1142570:CDS | 30.0% |
| TTAATAGCTCGGTAGGAAAT+CGG | - | chr7_4:17090630-17090649 | Msa1142570:CDS | 35.0% | |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_4:17090736-17090755 | Msa1142570:CDS | 35.0% |
| TAAGCAGATTATGCTTGCAA+TGG | - | chr7_4:17090862-17090881 | Msa1142570:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_4:17090908-17090927 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTG+AGG | + | chr7_4:17090940-17090959 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_4:17090597-17090616 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_4:17090607-17090626 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | - | chr7_4:17090623-17090642 | Msa1142570:CDS | 40.0% |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_4:17090688-17090707 | Msa1142570:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_4:17090693-17090712 | None:intergenic | 40.0% | |
| !!! | CGGGTTTTTGAAACACACTT+TGG | - | chr7_4:17090725-17090744 | Msa1142570:CDS | 40.0% |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_4:17090769-17090788 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_4:17090770-17090789 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_4:17090806-17090825 | Msa1142570:CDS | 40.0% | |
| ATTATGCTTGCAATGGCAGA+TGG | - | chr7_4:17090869-17090888 | Msa1142570:CDS | 40.0% | |
| TTATGCTTGCAATGGCAGAT+GGG | - | chr7_4:17090870-17090889 | Msa1142570:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_4:17090895-17090914 | Msa1142570:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_4:17090901-17090920 | Msa1142570:CDS | 40.0% | |
| CGGCTCGACACAAATAAAGA+TGG | - | chr7_4:17090659-17090678 | Msa1142570:CDS | 45.0% | |
| !! | CGGTAGGAAATCGGTTTGCT+CGG | - | chr7_4:17090639-17090658 | Msa1142570:CDS | 50.0% |
| TGAAGGAGCTCAAGAGCTTG+CGG | - | chr7_4:17090705-17090724 | Msa1142570:CDS | 50.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_4:17090875-17090894 | Msa1142570:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGC+GGG | - | chr7_4:17090706-17090725 | Msa1142570:CDS | 55.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_4:17090874-17090893 | Msa1142570:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 17090569 | 17090982 | 17090569 | ID=Msa1142570;Name=Msa1142570 |
| chr7_4 | mRNA | 17090569 | 17090982 | 17090569 | ID=Msa1142570-mRNA-1;Parent=Msa1142570;Name=Msa1142570-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_4 | exon | 17090569 | 17090982 | 17090569 | ID=Msa1142570-mRNA-1:exon:3835;Parent=Msa1142570-mRNA-1 |
| chr7_4 | CDS | 17090569 | 17090982 | 17090569 | ID=Msa1142570-mRNA-1:cds;Parent=Msa1142570-mRNA-1 |
| Gene Sequence |
| Protein sequence |
