Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095410 | XP_013447886.1 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 3.74e-90 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095410 | A0A072TYJ6 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 1.79e-90 | 268 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0034100 | Msa1095410 | -0.803556 | 3.294778e-49 | -8.615850e-47 |
| Msa0128340 | Msa1095410 | 0.807914 | 4.005145e-50 | -8.615850e-47 |
| Msa1011790 | Msa1095410 | 0.803356 | 3.624630e-49 | -8.615850e-47 |
| Msa1011800 | Msa1095410 | 0.900363 | 7.893817e-78 | -8.615850e-47 |
| Msa1011820 | Msa1095410 | 0.806569 | 7.717796e-50 | -8.615850e-47 |
| Msa1011830 | Msa1095410 | 0.802295 | 6.003757e-49 | -8.615850e-47 |
| Msa1011840 | Msa1095410 | 0.809401 | 1.927037e-50 | -8.615850e-47 |
| Msa1011860 | Msa1095410 | 0.814363 | 1.600509e-51 | -8.615850e-47 |
| Msa1011870 | Msa1095410 | 0.846782 | 1.748985e-59 | -8.615850e-47 |
| Msa1053300 | Msa1095410 | 0.807320 | 5.353130e-50 | -8.615850e-47 |
| Msa1053310 | Msa1095410 | 0.915084 | 8.952563e-85 | -8.615850e-47 |
| Msa1053330 | Msa1095410 | 0.828563 | 8.388257e-55 | -8.615850e-47 |
| Msa1053340 | Msa1095410 | 0.951180 | 3.512539e-109 | -8.615850e-47 |
| Msa1095400 | Msa1095410 | 0.835640 | 1.491427e-56 | -8.615850e-47 |
| Msa1095410 | Msa1142570 | 0.805769 | 1.137552e-49 | -8.615850e-47 |
| Msa1095410 | Msa1142580 | 0.809986 | 1.442393e-50 | -8.615850e-47 |
| Msa1095410 | Msa1142590 | 0.957021 | 7.398520e-115 | -8.615850e-47 |
| Msa1095410 | Msa1142600 | 0.807445 | 5.037019e-50 | -8.615850e-47 |
| Msa1095410 | Msa1146980 | 0.808199 | 3.482475e-50 | -8.615850e-47 |
| Msa1095410 | Msa1157210 | 0.805155 | 1.530451e-49 | -8.615850e-47 |
| Msa1095410 | Msa1197720 | 0.821174 | 4.646382e-53 | -8.615850e-47 |
| Msa1095410 | Msa1251410 | 0.802404 | 5.701327e-49 | -8.615850e-47 |
| Msa1095410 | Msa1429810 | 0.812282 | 4.584963e-51 | -8.615850e-47 |
| Msa1095410 | Msa1462780 | 0.804230 | 2.386653e-49 | -8.615850e-47 |
| Msa0882370 | Msa1095410 | 0.812587 | 3.932435e-51 | -8.615850e-47 |
| Msa0900830 | Msa1095410 | 0.805394 | 1.363474e-49 | -8.615850e-47 |
| Msa0926240 | Msa1095410 | 0.814835 | 1.258100e-51 | -8.615850e-47 |
| Msa0932140 | Msa1095410 | 0.807574 | 4.730211e-50 | -8.615850e-47 |
| Msa0932150 | Msa1095410 | 0.820340 | 7.224706e-53 | -8.615850e-47 |
| Msa0965800 | Msa1095410 | 0.803246 | 3.819468e-49 | -8.615850e-47 |
| Msa0970270 | Msa1095410 | 0.801429 | 9.043602e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095410 | MtrunA17_Chr7g0223391 | 95.620 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 7.63e-94 | 267 |
| Msa1095410 | MtrunA17_Chr7g0223401 | 94.891 | 137 | 7 | 0 | 1 | 137 | 6 | 142 | 1.38e-92 | 264 |
| Msa1095410 | MtrunA17_Chr8g0356181 | 47.857 | 140 | 70 | 1 | 1 | 137 | 1 | 140 | 8.69e-41 | 132 |
| Msa1095410 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 5.40e-20 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095410 | AT2G44310.1 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 9.62e-40 | 130 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_3:+18254198 | None:intergenic |
| CTTGAATGGCACAATTGATT+TGG | 0.268459 | 7_3:+18254453 | Msa1095410:CDS |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7_3:-18254400 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_3:-18254243 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_3:+18254534 | Msa1095410:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_3:-18254538 | None:intergenic |
| GAAGCATTTAATAGCTCAGT+TGG | 0.431408 | 7_3:+18254256 | Msa1095410:CDS |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_3:+18254263 | Msa1095410:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_3:-18254237 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_3:+18254528 | Msa1095410:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7_3:-18254399 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7_3:+18254339 | Msa1095410:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7_3:+18254439 | Msa1095410:CDS |
| CTCTTATGATGAGATGTTGA+AGG | 0.511648 | 7_3:+18254321 | Msa1095410:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_3:+18254507 | Msa1095410:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7_3:+18254338 | Msa1095410:CDS |
| ATGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7_3:+18254329 | Msa1095410:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7_3:+18254585 | Msa1095410:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_3:+18254508 | Msa1095410:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558215 | 7_3:+18254502 | Msa1095410:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_3:+18254369 | Msa1095410:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_3:-18254227 | None:intergenic |
| CGCCTCGACACAAACAGCGA+TGG | 0.598011 | 7_3:+18254292 | Msa1095410:CDS |
| AAAGCAGATGATGCTTGCAA+TGG | 0.609930 | 7_3:+18254495 | Msa1095410:CDS |
| GACCATCGCTGTTTGTGTCG+AGG | 0.621315 | 7_3:-18254294 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.625156 | 7_3:+18254503 | Msa1095410:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | + | chr7_3:18254202-18254221 | Msa1095410:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7_3:18254246-18254265 | None:intergenic | 30.0% | |
| TTAATAGCTCAGTTGGAAAT+CGG | + | chr7_3:18254263-18254282 | Msa1095410:CDS | 30.0% | |
| ! | TGAAAGAGAGTACAATAAAG+TGG | + | chr7_3:18254585-18254604 | Msa1095410:CDS | 30.0% |
| ! | GAAGCATTTAATAGCTCAGT+TGG | + | chr7_3:18254256-18254275 | Msa1095410:CDS | 35.0% |
| CTCTTATGATGAGATGTTGA+AGG | + | chr7_3:18254321-18254340 | Msa1095410:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | + | chr7_3:18254358-18254377 | Msa1095410:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | + | chr7_3:18254369-18254388 | Msa1095410:CDS | 35.0% |
| CTTGAATGGCACAATTGATT+TGG | + | chr7_3:18254453-18254472 | Msa1095410:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | - | chr7_3:18254541-18254560 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTG+AGG | - | chr7_3:18254573-18254592 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | - | chr7_3:18254230-18254249 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | - | chr7_3:18254240-18254259 | None:intergenic | 40.0% | |
| TTTCAAAAACCCTCAAGCTC+CGG | - | chr7_3:18254351-18254370 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | + | chr7_3:18254439-18254458 | Msa1095410:CDS | 40.0% | |
| AAAGCAGATGATGCTTGCAA+TGG | + | chr7_3:18254495-18254514 | Msa1095410:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7_3:18254528-18254547 | Msa1095410:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | + | chr7_3:18254534-18254553 | Msa1095410:CDS | 40.0% | |
| ATGAGATGTTGAAGGAGCTC+CGG | + | chr7_3:18254329-18254348 | Msa1095410:CDS | 45.0% | |
| AAACACGAGCAAGCTCGTTT+GGG | - | chr7_3:18254402-18254421 | None:intergenic | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | - | chr7_3:18254403-18254422 | None:intergenic | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7_3:18254502-18254521 | Msa1095410:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | + | chr7_3:18254503-18254522 | Msa1095410:CDS | 45.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | + | chr7_3:18254508-18254527 | Msa1095410:CDS | 50.0% | |
| GACCATCGCTGTTTGTGTCG+AGG | - | chr7_3:18254297-18254316 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | + | chr7_3:18254507-18254526 | Msa1095410:CDS | 55.0% | |
| CGCCTCGACACAAACAGCGA+TGG | + | chr7_3:18254292-18254311 | Msa1095410:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | + | chr7_3:18254338-18254357 | Msa1095410:CDS | 60.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | + | chr7_3:18254339-18254358 | Msa1095410:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 18254202 | 18254615 | 18254202 | ID=Msa1095410;Name=Msa1095410 |
| chr7_3 | mRNA | 18254202 | 18254615 | 18254202 | ID=Msa1095410-mRNA-1;Parent=Msa1095410;Name=Msa1095410-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_3 | exon | 18254202 | 18254615 | 18254202 | ID=Msa1095410-mRNA-1:exon:3960;Parent=Msa1095410-mRNA-1 |
| chr7_3 | CDS | 18254202 | 18254615 | 18254202 | ID=Msa1095410-mRNA-1:cds;Parent=Msa1095410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
