AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1142600


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1142600 MtrunA17_Chr7g0223401 94.161 137 8 0 1 137 6 142 2.48e-91 261
Msa1142600 MtrunA17_Chr7g0223391 92.701 137 10 0 1 137 18 154 1.55e-90 259
Msa1142600 MtrunA17_Chr8g0356181 48.571 140 69 1 1 137 1 140 2.45e-41 134
Msa1142600 MtrunA17_Chr1g0190921 35.878 131 79 1 1 131 1 126 1.24e-19 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1142600 AT2G44310.1 46.715 137 71 1 1 135 1 137 3.34e-39 129

Find 28 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGATGAGTGTAGAAATTT+TGG 0.159822 7_4:-17111523 None:intergenic
CTTCTTCATCTTCAAGGAAA+TGG 0.295262 7_4:+17111484 None:intergenic
TAAACACGAGCAAGCTCGTT+TGG 0.337682 7_4:+17111321 None:intergenic
TAAATGCTTCTTCATCTTCA+AGG 0.342383 7_4:+17111478 None:intergenic
CTTGCAATGGCAGATGGGTT+GGG 0.381320 7_4:-17111213 Msa1142600:CDS
CATGCCAATTCAAATGGTCT+TGG 0.391639 7_4:-17111187 Msa1142600:CDS
TCTTCCAAGACCATTTGAAT+TGG 0.395041 7_4:+17111183 None:intergenic
TTAATAGCTTGGTAGGTAAT+CGG 0.402120 7_4:-17111458 Msa1142600:CDS
GGGTTTCATGCCAATTCAAA+TGG 0.441784 7_4:-17111193 Msa1142600:CDS
TGAAGAAGCATTTAATAGCT+TGG 0.442582 7_4:-17111469 Msa1142600:CDS
CTCCTTCAACATCTCTTCAT+AGG 0.458078 7_4:+17111398 None:intergenic
GCTTGCAATGGCAGATGGGT+TGG 0.461788 7_4:-17111214 Msa1142600:CDS
AAACACGAGCAAGCTCGTTT+GGG 0.462526 7_4:+17111322 None:intergenic
GAAGGAGCTCCGGAGCTTGA+GGG 0.481338 7_4:-17111382 Msa1142600:CDS
CAGTTCGATCACAACTTGAA+TGG 0.485615 7_4:-17111282 Msa1142600:CDS
CTCCTATGAAGAGATGTTGA+AGG 0.486473 7_4:-17111400 Msa1142600:CDS
GAAGCATTTAATAGCTTGGT+AGG 0.494927 7_4:-17111465 Msa1142600:CDS
TTTCGAAAACCCTCAAGCTC+CGG 0.509695 7_4:+17111373 None:intergenic
AACACACTTTGGTATTGATG+TGG 0.520192 7_4:-17111352 Msa1142600:CDS
TGAAGGAGCTCCGGAGCTTG+AGG 0.522904 7_4:-17111383 Msa1142600:CDS
AAGAGATGTTGAAGGAGCTC+CGG 0.525969 7_4:-17111392 Msa1142600:CDS
TGAAAGAGAGTACAATAAAG+TGG 0.551009 7_4:-17111136 Msa1142600:CDS
ATGATGCTTGCAATGGCAGA+TGG 0.558625 7_4:-17111219 Msa1142600:CDS
GACCGTCGTTGTTTGTGTCG+AGG 0.574792 7_4:+17111427 None:intergenic
TTCAAGGAAATGGAAAATTG+TGG 0.612346 7_4:+17111494 None:intergenic
TAAGCAGATGATGCTTGCAA+TGG 0.622742 7_4:-17111226 Msa1142600:CDS
TGATGCTTGCAATGGCAGAT+GGG 0.629974 7_4:-17111218 Msa1142600:CDS
CGCCTCGACACAAACAACGA+CGG 0.676658 7_4:-17111429 Msa1142600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATGAGTGTAGAAATTTTGGA+TGG - chr7_4:17111128-17111147 Msa1142600:CDS 30.0%
! TGAGTGTAGAAATTTTGGAT+GGG - chr7_4:17111129-17111148 Msa1142600:CDS 30.0%
TTCAAGGAAATGGAAAATTG+TGG + chr7_4:17111156-17111175 None:intergenic 30.0%
TAAATGCTTCTTCATCTTCA+AGG + chr7_4:17111172-17111191 None:intergenic 30.0%
!! TGAAGAAGCATTTAATAGCT+TGG - chr7_4:17111178-17111197 Msa1142600:CDS 30.0%
TTAATAGCTTGGTAGGTAAT+CGG - chr7_4:17111189-17111208 Msa1142600:CDS 30.0%
! TGAAAGAGAGTACAATAAAG+TGG - chr7_4:17111511-17111530 Msa1142600:CDS 30.0%
! GAGTGTAGAAATTTTGGATG+GGG - chr7_4:17111130-17111149 Msa1142600:CDS 35.0%
CTTCTTCATCTTCAAGGAAA+TGG + chr7_4:17111166-17111185 None:intergenic 35.0%
! GAAGCATTTAATAGCTTGGT+AGG - chr7_4:17111182-17111201 Msa1142600:CDS 35.0%
!! AACACACTTTGGTATTGATG+TGG - chr7_4:17111295-17111314 Msa1142600:CDS 35.0%
TCTTCCAAGACCATTTGAAT+TGG + chr7_4:17111467-17111486 None:intergenic 35.0%
!! CTCTTTCAACAGCTATTTTG+AGG + chr7_4:17111499-17111518 None:intergenic 35.0%
CTCCTATGAAGAGATGTTGA+AGG - chr7_4:17111247-17111266 Msa1142600:CDS 40.0%
CTCCTTCAACATCTCTTCAT+AGG + chr7_4:17111252-17111271 None:intergenic 40.0%
! AGGGTTTTCGAAACACACTT+TGG - chr7_4:17111284-17111303 Msa1142600:CDS 40.0%
CAGTTCGATCACAACTTGAA+TGG - chr7_4:17111365-17111384 Msa1142600:CDS 40.0%
TAAGCAGATGATGCTTGCAA+TGG - chr7_4:17111421-17111440 Msa1142600:CDS 40.0%
GGGTTTCATGCCAATTCAAA+TGG - chr7_4:17111454-17111473 Msa1142600:CDS 40.0%
CATGCCAATTCAAATGGTCT+TGG - chr7_4:17111460-17111479 Msa1142600:CDS 40.0%
AAGAGATGTTGAAGGAGCTC+CGG - chr7_4:17111255-17111274 Msa1142600:CDS 45.0%
TTTCGAAAACCCTCAAGCTC+CGG + chr7_4:17111277-17111296 None:intergenic 45.0%
AAACACGAGCAAGCTCGTTT+GGG + chr7_4:17111328-17111347 None:intergenic 45.0%
TAAACACGAGCAAGCTCGTT+TGG + chr7_4:17111329-17111348 None:intergenic 45.0%
ATGATGCTTGCAATGGCAGA+TGG - chr7_4:17111428-17111447 Msa1142600:CDS 45.0%
TGATGCTTGCAATGGCAGAT+GGG - chr7_4:17111429-17111448 Msa1142600:CDS 45.0%
! CTTGCAATGGCAGATGGGTT+GGG - chr7_4:17111434-17111453 Msa1142600:CDS 50.0%
CGCCTCGACACAAACAACGA+CGG - chr7_4:17111218-17111237 Msa1142600:CDS 55.0%
GACCGTCGTTGTTTGTGTCG+AGG + chr7_4:17111223-17111242 None:intergenic 55.0%
GCTTGCAATGGCAGATGGGT+TGG - chr7_4:17111433-17111452 Msa1142600:CDS 55.0%
TGAAGGAGCTCCGGAGCTTG+AGG - chr7_4:17111264-17111283 Msa1142600:CDS 60.0%
GAAGGAGCTCCGGAGCTTGA+GGG - chr7_4:17111265-17111284 Msa1142600:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 17111128 17111541 17111128 ID=Msa1142600;Name=Msa1142600
chr7_4 mRNA 17111128 17111541 17111128 ID=Msa1142600-mRNA-1;Parent=Msa1142600;Name=Msa1142600-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|137
chr7_4 exon 17111128 17111541 17111128 ID=Msa1142600-mRNA-1:exon:3837;Parent=Msa1142600-mRNA-1
chr7_4 CDS 17111128 17111541 17111128 ID=Msa1142600-mRNA-1:cds;Parent=Msa1142600-mRNA-1
Gene Sequence

>Msa1142600

ATGAGTGTAGAAATTTTGGATGGGGCCACAATTTTCCATTTCCTTGAAGATGAAGAAGCATTTAATAGCTTGGTAGGTAATCGGTTTGCTCGCCTCGACACAAACAACGACGGTCTTCTCTCCTATGAAGAGATGTTGAAGGAGCTCCGGAGCTTGAGGGTTTTCGAAACACACTTTGGTATTGATGTGGATTCAGACCCAAACGAGCTTGCTCGTGTTTATGAATCTTTGTTCATACAGTTCGATCACAACTTGAATGGCACAATTGATCTCGAAGAATTCAAGAAAGAAACTAAGCAGATGATGCTTGCAATGGCAGATGGGTTGGGTTTCATGCCAATTCAAATGGTCTTGGAAGAAGACAGCATCCTCAAAATAGCTGTTGAAAGAGAGTACAATAAAGTGGCAGCTTAA

Protein sequence

>Msa1142600

MSVEILDGATIFHFLEDEEAFNSLVGNRFARLDTNNDGLLSYEEMLKELRSLRVFETHFGIDVDSDPNELARVYESLFIQFDHNLNGTIDLEEFKKETKQMMLAMADGLGFMPIQMVLEEDSILKIAVEREYNKVAA*