AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1156560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1156560 MtrunA17_Chr7g0250141 58.896 326 98 8 6 300 1 321 1.21e-112 328
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 81 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACGTTTGTGCTGAAATTT+AGG 0.171556 7_4:+44883417 None:intergenic
ACGGTTTCCTTGTGCTTATT+TGG 0.219708 7_4:+44883453 None:intergenic
GTAATCTTGACCATTATATT+TGG 0.230664 7_4:-44883366 Msa1156560:CDS
TATAGTTAGCATTATCTAAT+TGG 0.231671 7_4:-44882823 Msa1156560:three_prime_UTR
TCCATATGCACCATGATTTC+CGG 0.234037 7_4:-44882863 Msa1156560:three_prime_UTR
TACTCGGAATGGTTGTCTTT+GGG 0.248890 7_4:-44883113 Msa1156560:CDS
AGGTCCAACGTGGTCAAATA+TGG 0.249406 7_4:-44883302 Msa1156560:CDS
CATCCACCAAGAGGATTTCT+TGG 0.279495 7_4:+44884076 None:intergenic
GAGTGTTGTGTTTATCTTCT+GGG 0.288933 7_4:-44883190 Msa1156560:CDS
AATCTTAAAGCCATTCATTA+TGG 0.289423 7_4:-44883895 Msa1156560:CDS
CCTCTTTATCAACACAAATA+AGG 0.303589 7_4:+44883610 None:intergenic
ATACTCGGAATGGTTGTCTT+TGG 0.312772 7_4:-44883114 Msa1156560:CDS
TAGCTTCGTTCAAGATTGTT+AGG 0.328816 7_4:-44884509 Msa1156560:intron
TTCAAGGACATGCTGCAATT+TGG 0.337988 7_4:-44884111 Msa1156560:CDS
AGAGTGTTGTGTTTATCTTC+TGG 0.340356 7_4:-44883191 Msa1156560:CDS
TGAGCAACTTACCTGGATAT+TGG 0.346014 7_4:+44883861 None:intergenic
CGATCCACGTGTCGAAGAAA+AGG 0.367683 7_4:+44883340 None:intergenic
GAAAAGGGAACCAAATATAA+TGG 0.381996 7_4:+44883356 None:intergenic
ATACTCTATAAAAGTATGTA+AGG 0.405060 7_4:+44883653 None:intergenic
AAAATTAGCTGAAGACTTCA+AGG 0.407924 7_4:-44884127 Msa1156560:CDS
AGGAACTTACTATGATTTGT+TGG 0.419337 7_4:+44883630 None:intergenic
CCTTATTTGTGTTGATAAAG+AGG 0.421351 7_4:-44883610 Msa1156560:CDS
TGAAATTTAGGATATGATAT+TGG 0.422942 7_4:+44883429 None:intergenic
CTGCTTTGCGTATTGCTGTT+GGG 0.429582 7_4:-44882969 Msa1156560:three_prime_UTR
TAATTAAGACAAATGCTTTG+TGG 0.431922 7_4:-44882929 Msa1156560:three_prime_UTR
GCCGGAAATCATGGTGCATA+TGG 0.435809 7_4:+44882862 None:intergenic
GACCACGTTGGACCTTCATA+AGG 0.436592 7_4:+44883310 None:intergenic
ATGTGAACCTGAGATATTAC+AGG 0.439567 7_4:+44884680 None:intergenic
AATTTGATAAGATGGATATA+AGG 0.440936 7_4:-44884170 Msa1156560:exon
ACCACGTTGGACCTTCATAA+GGG 0.454092 7_4:+44883311 None:intergenic
TTGCTGTTGGGCACTAAACT+TGG 0.457266 7_4:-44882957 Msa1156560:three_prime_UTR
ACAGGGGAAATTTGATAAGA+TGG 0.457412 7_4:-44884178 Msa1156560:intron
ACTCGGAATGGTTGTCTTTG+GGG 0.473197 7_4:-44883112 Msa1156560:CDS
AGATTCTAAATATATTTGTC+AGG 0.473876 7_4:+44884599 None:intergenic
GAAGAAACAAATCATCGTGT+TGG 0.475250 7_4:-44884016 Msa1156560:CDS
AAGACAACCATTCCGAGTAT+TGG 0.477069 7_4:+44883117 None:intergenic
AATCTTAGAATGTTGATTTG+AGG 0.478486 7_4:+44884565 None:intergenic
AAGGACATGCTGCAATTTGG+TGG 0.486636 7_4:-44884108 Msa1156560:CDS
TCGAAATCCTGTAATATCTC+AGG 0.486803 7_4:-44884687 Msa1156560:five_prime_UTR
ACTGCTTTGCGTATTGCTGT+TGG 0.491166 7_4:-44882970 Msa1156560:three_prime_UTR
ATGTTTACGATTTCACGCTT+AGG 0.493571 7_4:-44883919 Msa1156560:CDS
GTTGCTATAATGATTCTTGT+TGG 0.493716 7_4:-44883234 Msa1156560:CDS
TAATGTAATATACCTTTCCT+TGG 0.499043 7_4:-44882997 Msa1156560:three_prime_UTR
TTTGAAGCCTTCCAATATCC+AGG 0.501125 7_4:-44883872 Msa1156560:intron
AAAGTGCTGGCCGGAAATCA+TGG 0.508063 7_4:+44882853 None:intergenic
AAATATGGGTCTCCTCGCTC+AGG 0.509899 7_4:-44883287 Msa1156560:CDS
GATCCACGTGTCGAAGAAAA+GGG 0.523570 7_4:+44883341 None:intergenic
GGTCCAACGTGGTCAAATAT+GGG 0.526486 7_4:-44883301 Msa1156560:CDS
ACACAACCATTAGCCACGGT+TGG 0.528420 7_4:-44883522 Msa1156560:CDS
AGGCTTCAAACCATAATGAA+TGG 0.532125 7_4:+44883885 None:intergenic
GAACCAAGAAATCCTCTTGG+TGG 0.533519 7_4:-44884079 Msa1156560:CDS
GACGAATGCATCACCACCGT+TGG 0.543227 7_4:-44882891 Msa1156560:three_prime_UTR
TAAATTTCAGCACAAACGTA+AGG 0.550229 7_4:-44883415 Msa1156560:CDS
ATTTGGATTATTACTACTAG+TGG 0.555390 7_4:+44883470 None:intergenic
GTCTTTGGGGTGTTTGCTCG+TGG 0.556626 7_4:-44883099 Msa1156560:CDS
AGCAAATAAAGAGCACTGCA+TGG 0.561104 7_4:-44883587 Msa1156560:CDS
TGAGAACCAAGAAATCCTCT+TGG 0.562798 7_4:-44884082 Msa1156560:CDS
CAACTTACCTGGATATTGGA+AGG 0.568102 7_4:+44883865 None:intergenic
AAAATCAAGAGTTGATCAGA+AGG 0.572726 7_4:-44883981 Msa1156560:CDS
ATGTTGATTTGAGGATGAAA+CGG 0.585785 7_4:+44884574 None:intergenic
GATCGAGCCATCCCTTATGA+AGG 0.587003 7_4:-44883322 Msa1156560:CDS
ACACTCTGCACACGGACGAA+TGG 0.589264 7_4:+44883207 None:intergenic
AACAAGAATCATTATAGCAA+CGG 0.594551 7_4:+44883236 None:intergenic
TTTGTGGTACGATACGAGAA+TGG 0.597174 7_4:-44882913 Msa1156560:three_prime_UTR
ACAATGTGTGTTCCAATACT+CGG 0.599011 7_4:-44883129 Msa1156560:CDS
CGTTGGACCTTCATAAGGGA+TGG 0.600614 7_4:+44883315 None:intergenic
TTTCAAGTTCATCCACCAAG+AGG 0.609797 7_4:+44884067 None:intergenic
TACTCTATAAAAGTATGTAA+GGG 0.610014 7_4:+44883654 None:intergenic
TAAACACAACACTCTGCACA+CGG 0.624793 7_4:+44883199 None:intergenic
TTACTTTGAATCAGTCCATG+TGG 0.639089 7_4:-44884722 Msa1156560:five_prime_UTR
GTGTGTTCCAATACTCGGAA+TGG 0.643820 7_4:-44883124 Msa1156560:CDS
CGACACATCAAATGACCACA+TGG 0.645456 7_4:+44884707 None:intergenic
TTTAGGATATGATATTGGCA+CGG 0.659698 7_4:+44883434 None:intergenic
ATTTACACAACCATTAGCCA+CGG 0.664300 7_4:-44883526 Msa1156560:CDS
TACGCAAAGCAGTACTACCA+AGG 0.669016 7_4:+44882980 None:intergenic
AGACCCATATTTGACCACGT+TGG 0.670283 7_4:+44883298 None:intergenic
GTGCATATGGAATAAACCAA+CGG 0.670297 7_4:+44882875 None:intergenic
AAAGCAGTACTACCAAGGAA+AGG 0.672840 7_4:+44882985 None:intergenic
TAATAATCCAAATAAGCACA+AGG 0.676862 7_4:-44883460 Msa1156560:CDS
TCCCTTATGAAGGTCCAACG+TGG 0.728134 7_4:-44883312 Msa1156560:CDS
CATATGGAATAAACCAACGG+TGG 0.747921 7_4:+44882878 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAATCAAAACATTTCA+AGG + chr7_4:44883158-44883177 None:intergenic 15.0%
!!! TACACATTTCTTTTTTTTTA+TGG - chr7_4:44883362-44883381 Msa1156560:CDS 15.0%
!! TATTAAATCAAAACATTTCA+AGG + chr7_4:44883158-44883177 None:intergenic 15.0%
!!! TACACATTTCTTTTTTTTTA+TGG - chr7_4:44883362-44883381 Msa1156560:CDS 15.0%
!! AAAAAGATGAAACAGATTAT+GGG + chr7_4:44882918-44882937 None:intergenic 20.0%
!! TAAAAAGATGAAACAGATTA+TGG + chr7_4:44882919-44882938 None:intergenic 20.0%
!! AGATTCTAAATATATTTGTC+AGG + chr7_4:44882970-44882989 None:intergenic 20.0%
!!! TCCAATTTTTTTTTTCTTTC+TGG - chr7_4:44883123-44883142 Msa1156560:CDS 20.0%
!! TCCAGAAAGAAAAAAAAAAT+TGG + chr7_4:44883127-44883146 None:intergenic 20.0%
!!! TCTTTTTTTTTATGGATAAC+AGG - chr7_4:44883370-44883389 Msa1156560:CDS 20.0%
!!! CTTTTTTTTTATGGATAACA+GGG - chr7_4:44883371-44883390 Msa1156560:CDS 20.0%
!!! TTTTTTTTTATGGATAACAG+GGG - chr7_4:44883372-44883391 Msa1156560:CDS 20.0%
!! AATTTGATAAGATGGATATA+AGG - chr7_4:44883396-44883415 Msa1156560:CDS 20.0%
!!! TACTCTATAAAAGTATGTAA+GGG + chr7_4:44883915-44883934 None:intergenic 20.0%
!!! ATACTCTATAAAAGTATGTA+AGG + chr7_4:44883916-44883935 None:intergenic 20.0%
!! AATTCAATGTAAAAAACACA+CGG + chr7_4:44884023-44884042 None:intergenic 20.0%
!!! TGAAATTTAGGATATGATAT+TGG + chr7_4:44884140-44884159 None:intergenic 20.0%
!!! TGGAAAATTTTAGAGAATAT+TGG - chr7_4:44884487-44884506 Msa1156560:intron 20.0%
!! TATAGTTAGCATTATCTAAT+TGG - chr7_4:44884743-44884762 Msa1156560:five_prime_UTR 20.0%
!! AAAAAGATGAAACAGATTAT+GGG + chr7_4:44882918-44882937 None:intergenic 20.0%
!! TAAAAAGATGAAACAGATTA+TGG + chr7_4:44882919-44882938 None:intergenic 20.0%
!! AGATTCTAAATATATTTGTC+AGG + chr7_4:44882970-44882989 None:intergenic 20.0%
!!! TCCAATTTTTTTTTTCTTTC+TGG - chr7_4:44883123-44883142 Msa1156560:CDS 20.0%
!! TCCAGAAAGAAAAAAAAAAT+TGG + chr7_4:44883127-44883146 None:intergenic 20.0%
!!! TCTTTTTTTTTATGGATAAC+AGG - chr7_4:44883370-44883389 Msa1156560:CDS 20.0%
!!! CTTTTTTTTTATGGATAACA+GGG - chr7_4:44883371-44883390 Msa1156560:CDS 20.0%
!!! TTTTTTTTTATGGATAACAG+GGG - chr7_4:44883372-44883391 Msa1156560:CDS 20.0%
!! AATTTGATAAGATGGATATA+AGG - chr7_4:44883396-44883415 Msa1156560:CDS 20.0%
!!! TACTCTATAAAAGTATGTAA+GGG + chr7_4:44883915-44883934 None:intergenic 20.0%
!!! ATACTCTATAAAAGTATGTA+AGG + chr7_4:44883916-44883935 None:intergenic 20.0%
!! AATTCAATGTAAAAAACACA+CGG + chr7_4:44884023-44884042 None:intergenic 20.0%
!!! TGAAATTTAGGATATGATAT+TGG + chr7_4:44884140-44884159 None:intergenic 20.0%
!!! TGGAAAATTTTAGAGAATAT+TGG - chr7_4:44884487-44884506 Msa1156560:intron 20.0%
!! TATAGTTAGCATTATCTAAT+TGG - chr7_4:44884743-44884762 Msa1156560:five_prime_UTR 20.0%
!! ATAACTTTTACTGTGGAATT+TGG + chr7_4:44882947-44882966 None:intergenic 25.0%
!!! AATCTTAGAATGTTGATTTG+AGG + chr7_4:44883004-44883023 None:intergenic 25.0%
! AAAAAATTGGACAAGAGAAT+AGG + chr7_4:44883114-44883133 None:intergenic 25.0%
! TTCATAAACCCTAACAATAT+AGG - chr7_4:44883244-44883263 Msa1156560:CDS 25.0%
! TTGACTATTGTAATTGTAGA+TGG - chr7_4:44883306-44883325 Msa1156560:CDS 25.0%
! AATCTTAAAGCCATTCATTA+TGG - chr7_4:44883671-44883690 Msa1156560:intron 25.0%
! TAAAAGTCGGTCAAAAAAAA+TGG + chr7_4:44883773-44883792 None:intergenic 25.0%
! CTAAGGATAATAGTAAAAGT+CGG + chr7_4:44883786-44883805 None:intergenic 25.0%
! ATTGAATATCTGTCTTTCTA+AGG + chr7_4:44883803-44883822 None:intergenic 25.0%
! GAAAGACAGATATTCAATTA+AGG - chr7_4:44883805-44883824 Msa1156560:intron 25.0%
! ATATTCAATTAAGGAAATCC+AGG - chr7_4:44883814-44883833 Msa1156560:intron 25.0%
! ATTTGGATTATTACTACTAG+TGG + chr7_4:44884099-44884118 None:intergenic 25.0%
! TAATAATCCAAATAAGCACA+AGG - chr7_4:44884106-44884125 Msa1156560:CDS 25.0%
! GTAATCTTGACCATTATATT+TGG - chr7_4:44884200-44884219 Msa1156560:intron 25.0%
! AACAAGAATCATTATAGCAA+CGG + chr7_4:44884333-44884352 None:intergenic 25.0%
! TAATGTAATATACCTTTCCT+TGG - chr7_4:44884569-44884588 Msa1156560:five_prime_UTR 25.0%
! TAATTAAGACAAATGCTTTG+TGG - chr7_4:44884637-44884656 Msa1156560:five_prime_UTR 25.0%
!! ATAACTTTTACTGTGGAATT+TGG + chr7_4:44882947-44882966 None:intergenic 25.0%
!!! AATCTTAGAATGTTGATTTG+AGG + chr7_4:44883004-44883023 None:intergenic 25.0%
! AAAAAATTGGACAAGAGAAT+AGG + chr7_4:44883114-44883133 None:intergenic 25.0%
! TTCATAAACCCTAACAATAT+AGG - chr7_4:44883244-44883263 Msa1156560:CDS 25.0%
! TTGACTATTGTAATTGTAGA+TGG - chr7_4:44883306-44883325 Msa1156560:CDS 25.0%
! AATCTTAAAGCCATTCATTA+TGG - chr7_4:44883671-44883690 Msa1156560:intron 25.0%
! TAAAAGTCGGTCAAAAAAAA+TGG + chr7_4:44883773-44883792 None:intergenic 25.0%
! CTAAGGATAATAGTAAAAGT+CGG + chr7_4:44883786-44883805 None:intergenic 25.0%
! ATTGAATATCTGTCTTTCTA+AGG + chr7_4:44883803-44883822 None:intergenic 25.0%
! GAAAGACAGATATTCAATTA+AGG - chr7_4:44883805-44883824 Msa1156560:intron 25.0%
! ATATTCAATTAAGGAAATCC+AGG - chr7_4:44883814-44883833 Msa1156560:intron 25.0%
! ATTTGGATTATTACTACTAG+TGG + chr7_4:44884099-44884118 None:intergenic 25.0%
! TAATAATCCAAATAAGCACA+AGG - chr7_4:44884106-44884125 Msa1156560:CDS 25.0%
! GTAATCTTGACCATTATATT+TGG - chr7_4:44884200-44884219 Msa1156560:intron 25.0%
! AACAAGAATCATTATAGCAA+CGG + chr7_4:44884333-44884352 None:intergenic 25.0%
! TAATGTAATATACCTTTCCT+TGG - chr7_4:44884569-44884588 Msa1156560:five_prime_UTR 25.0%
! TAATTAAGACAAATGCTTTG+TGG - chr7_4:44884637-44884656 Msa1156560:five_prime_UTR 25.0%
!! ATGTTGATTTGAGGATGAAA+CGG + chr7_4:44882995-44883014 None:intergenic 30.0%
AAGAGAATAGGAAATTGGAA+TGG + chr7_4:44883102-44883121 None:intergenic 30.0%
TGCTACATCCTATATTGTTA+GGG + chr7_4:44883255-44883274 None:intergenic 30.0%
ATGCTACATCCTATATTGTT+AGG + chr7_4:44883256-44883275 None:intergenic 30.0%
AAAATTAGCTGAAGACTTCA+AGG - chr7_4:44883439-44883458 Msa1156560:CDS 30.0%
! AAAATCAAGAGTTGATCAGA+AGG - chr7_4:44883585-44883604 Msa1156560:CDS 30.0%
CAAAAAATCAAAATGACTCC+TGG + chr7_4:44883835-44883854 None:intergenic 30.0%
!!! CAGGAGTCATTTTGATTTTT+TGG - chr7_4:44883833-44883852 Msa1156560:intron 30.0%
! AGGAACTTACTATGATTTGT+TGG + chr7_4:44883939-44883958 None:intergenic 30.0%
CCTCTTTATCAACACAAATA+AGG + chr7_4:44883959-44883978 None:intergenic 30.0%
! CCTTATTTGTGTTGATAAAG+AGG - chr7_4:44883956-44883975 Msa1156560:CDS 30.0%
! TTTAGGATATGATATTGGCA+CGG + chr7_4:44884135-44884154 None:intergenic 30.0%
TTACGTTTGTGCTGAAATTT+AGG + chr7_4:44884152-44884171 None:intergenic 30.0%
TAAATTTCAGCACAAACGTA+AGG - chr7_4:44884151-44884170 Msa1156560:CDS 30.0%
GAAAAGGGAACCAAATATAA+TGG + chr7_4:44884213-44884232 None:intergenic 30.0%
!!! TTGGTTTTATGGCATTTGTT+TGG - chr7_4:44884306-44884325 Msa1156560:intron 30.0%
!!! TGGTTTTATGGCATTTGTTT+GGG - chr7_4:44884307-44884326 Msa1156560:intron 30.0%
GTTGCTATAATGATTCTTGT+TGG - chr7_4:44884332-44884351 Msa1156560:intron 30.0%
ACTATAAAAAGTCAAAGTGC+TGG + chr7_4:44884729-44884748 None:intergenic 30.0%
!! ATGTTGATTTGAGGATGAAA+CGG + chr7_4:44882995-44883014 None:intergenic 30.0%
AAGAGAATAGGAAATTGGAA+TGG + chr7_4:44883102-44883121 None:intergenic 30.0%
TGCTACATCCTATATTGTTA+GGG + chr7_4:44883255-44883274 None:intergenic 30.0%
ATGCTACATCCTATATTGTT+AGG + chr7_4:44883256-44883275 None:intergenic 30.0%
AAAATTAGCTGAAGACTTCA+AGG - chr7_4:44883439-44883458 Msa1156560:CDS 30.0%
! AAAATCAAGAGTTGATCAGA+AGG - chr7_4:44883585-44883604 Msa1156560:CDS 30.0%
CAAAAAATCAAAATGACTCC+TGG + chr7_4:44883835-44883854 None:intergenic 30.0%
!!! CAGGAGTCATTTTGATTTTT+TGG - chr7_4:44883833-44883852 Msa1156560:intron 30.0%
! AGGAACTTACTATGATTTGT+TGG + chr7_4:44883939-44883958 None:intergenic 30.0%
CCTCTTTATCAACACAAATA+AGG + chr7_4:44883959-44883978 None:intergenic 30.0%
! CCTTATTTGTGTTGATAAAG+AGG - chr7_4:44883956-44883975 Msa1156560:CDS 30.0%
! TTTAGGATATGATATTGGCA+CGG + chr7_4:44884135-44884154 None:intergenic 30.0%
TTACGTTTGTGCTGAAATTT+AGG + chr7_4:44884152-44884171 None:intergenic 30.0%
TAAATTTCAGCACAAACGTA+AGG - chr7_4:44884151-44884170 Msa1156560:CDS 30.0%
GAAAAGGGAACCAAATATAA+TGG + chr7_4:44884213-44884232 None:intergenic 30.0%
!!! TTGGTTTTATGGCATTTGTT+TGG - chr7_4:44884306-44884325 Msa1156560:intron 30.0%
!!! TGGTTTTATGGCATTTGTTT+GGG - chr7_4:44884307-44884326 Msa1156560:intron 30.0%
GTTGCTATAATGATTCTTGT+TGG - chr7_4:44884332-44884351 Msa1156560:intron 30.0%
ACTATAAAAAGTCAAAGTGC+TGG + chr7_4:44884729-44884748 None:intergenic 30.0%
TTACTTTGAATCAGTCCATG+TGG - chr7_4:44882844-44882863 Msa1156560:three_prime_UTR 35.0%
TCGAAATCCTGTAATATCTC+AGG - chr7_4:44882879-44882898 Msa1156560:three_prime_UTR 35.0%
ATGTGAACCTGAGATATTAC+AGG + chr7_4:44882889-44882908 None:intergenic 35.0%
! TGTCAGGATAACTTTTACTG+TGG + chr7_4:44882954-44882973 None:intergenic 35.0%
TAGCTTCGTTCAAGATTGTT+AGG - chr7_4:44883057-44883076 Msa1156560:three_prime_UTR 35.0%
TGGACAAGAGAATAGGAAAT+TGG + chr7_4:44883107-44883126 None:intergenic 35.0%
ACAGGGGAAATTTGATAAGA+TGG - chr7_4:44883388-44883407 Msa1156560:CDS 35.0%
GAAGAAACAAATCATCGTGT+TGG - chr7_4:44883550-44883569 Msa1156560:CDS 35.0%
ATGTTTACGATTTCACGCTT+AGG - chr7_4:44883647-44883666 Msa1156560:CDS 35.0%
AGGCTTCAAACCATAATGAA+TGG + chr7_4:44883684-44883703 None:intergenic 35.0%
! AATAAGTTGAGCAACTTACC+TGG + chr7_4:44883715-44883734 None:intergenic 35.0%
TCGGTCAAAAAAAATGGTGA+TGG + chr7_4:44883767-44883786 None:intergenic 35.0%
GTGTATAGAAAGTTAGAAGG+AGG + chr7_4:44883881-44883900 None:intergenic 35.0%
GCAGTGTATAGAAAGTTAGA+AGG + chr7_4:44883884-44883903 None:intergenic 35.0%
ATTTACACAACCATTAGCCA+CGG - chr7_4:44884040-44884059 Msa1156560:CDS 35.0%
!! AGAGTGTTGTGTTTATCTTC+TGG - chr7_4:44884375-44884394 Msa1156560:intron 35.0%
! GAGTGTTGTGTTTATCTTCT+GGG - chr7_4:44884376-44884395 Msa1156560:intron 35.0%
ACAATGTGTGTTCCAATACT+CGG - chr7_4:44884437-44884456 Msa1156560:intron 35.0%
GTGCATATGGAATAAACCAA+CGG + chr7_4:44884694-44884713 None:intergenic 35.0%
TTACTTTGAATCAGTCCATG+TGG - chr7_4:44882844-44882863 Msa1156560:three_prime_UTR 35.0%
TCGAAATCCTGTAATATCTC+AGG - chr7_4:44882879-44882898 Msa1156560:three_prime_UTR 35.0%
ATGTGAACCTGAGATATTAC+AGG + chr7_4:44882889-44882908 None:intergenic 35.0%
! TGTCAGGATAACTTTTACTG+TGG + chr7_4:44882954-44882973 None:intergenic 35.0%
TAGCTTCGTTCAAGATTGTT+AGG - chr7_4:44883057-44883076 Msa1156560:three_prime_UTR 35.0%
TGGACAAGAGAATAGGAAAT+TGG + chr7_4:44883107-44883126 None:intergenic 35.0%
ACAGGGGAAATTTGATAAGA+TGG - chr7_4:44883388-44883407 Msa1156560:CDS 35.0%
GAAGAAACAAATCATCGTGT+TGG - chr7_4:44883550-44883569 Msa1156560:CDS 35.0%
ATGTTTACGATTTCACGCTT+AGG - chr7_4:44883647-44883666 Msa1156560:CDS 35.0%
AGGCTTCAAACCATAATGAA+TGG + chr7_4:44883684-44883703 None:intergenic 35.0%
! AATAAGTTGAGCAACTTACC+TGG + chr7_4:44883715-44883734 None:intergenic 35.0%
TCGGTCAAAAAAAATGGTGA+TGG + chr7_4:44883767-44883786 None:intergenic 35.0%
GTGTATAGAAAGTTAGAAGG+AGG + chr7_4:44883881-44883900 None:intergenic 35.0%
GCAGTGTATAGAAAGTTAGA+AGG + chr7_4:44883884-44883903 None:intergenic 35.0%
ATTTACACAACCATTAGCCA+CGG - chr7_4:44884040-44884059 Msa1156560:CDS 35.0%
!! AGAGTGTTGTGTTTATCTTC+TGG - chr7_4:44884375-44884394 Msa1156560:intron 35.0%
! GAGTGTTGTGTTTATCTTCT+GGG - chr7_4:44884376-44884395 Msa1156560:intron 35.0%
ACAATGTGTGTTCCAATACT+CGG - chr7_4:44884437-44884456 Msa1156560:intron 35.0%
GTGCATATGGAATAAACCAA+CGG + chr7_4:44884694-44884713 None:intergenic 35.0%
TTCAAGGACATGCTGCAATT+TGG - chr7_4:44883455-44883474 Msa1156560:CDS 40.0%
TGAGAACCAAGAAATCCTCT+TGG - chr7_4:44883484-44883503 Msa1156560:CDS 40.0%
!! TTTCAAGTTCATCCACCAAG+AGG + chr7_4:44883502-44883521 None:intergenic 40.0%
! TTTGAAGCCTTCCAATATCC+AGG - chr7_4:44883694-44883713 Msa1156560:intron 40.0%
CAACTTACCTGGATATTGGA+AGG + chr7_4:44883704-44883723 None:intergenic 40.0%
TGAGCAACTTACCTGGATAT+TGG + chr7_4:44883708-44883727 None:intergenic 40.0%
!! AGCAAATAAAGAGCACTGCA+TGG - chr7_4:44883979-44883998 Msa1156560:CDS 40.0%
! ATTTTTCCAACCGTGGCTAA+TGG + chr7_4:44884053-44884072 None:intergenic 40.0%
!!! TGAAGTCATTTTTCCAACCG+TGG + chr7_4:44884060-44884079 None:intergenic 40.0%
ACGGTTTCCTTGTGCTTATT+TGG + chr7_4:44884116-44884135 None:intergenic 40.0%
!!! GCTCAGGCTTTTTGGTTTTA+TGG - chr7_4:44884295-44884314 Msa1156560:intron 40.0%
TAAACACAACACTCTGCACA+CGG + chr7_4:44884370-44884389 None:intergenic 40.0%
AAGACAACCATTCCGAGTAT+TGG + chr7_4:44884452-44884471 None:intergenic 40.0%
ATACTCGGAATGGTTGTCTT+TGG - chr7_4:44884452-44884471 Msa1156560:intron 40.0%
TACTCGGAATGGTTGTCTTT+GGG - chr7_4:44884453-44884472 Msa1156560:intron 40.0%
AAAGCAGTACTACCAAGGAA+AGG + chr7_4:44884584-44884603 None:intergenic 40.0%
TTTGTGGTACGATACGAGAA+TGG - chr7_4:44884653-44884672 Msa1156560:five_prime_UTR 40.0%
CATATGGAATAAACCAACGG+TGG + chr7_4:44884691-44884710 None:intergenic 40.0%
TCCATATGCACCATGATTTC+CGG - chr7_4:44884703-44884722 Msa1156560:five_prime_UTR 40.0%
!! TAAAAAGTCAAAGTGCTGGC+CGG + chr7_4:44884725-44884744 None:intergenic 40.0%
TTCAAGGACATGCTGCAATT+TGG - chr7_4:44883455-44883474 Msa1156560:CDS 40.0%
TGAGAACCAAGAAATCCTCT+TGG - chr7_4:44883484-44883503 Msa1156560:CDS 40.0%
!! TTTCAAGTTCATCCACCAAG+AGG + chr7_4:44883502-44883521 None:intergenic 40.0%
! TTTGAAGCCTTCCAATATCC+AGG - chr7_4:44883694-44883713 Msa1156560:intron 40.0%
CAACTTACCTGGATATTGGA+AGG + chr7_4:44883704-44883723 None:intergenic 40.0%
TGAGCAACTTACCTGGATAT+TGG + chr7_4:44883708-44883727 None:intergenic 40.0%
!! AGCAAATAAAGAGCACTGCA+TGG - chr7_4:44883979-44883998 Msa1156560:CDS 40.0%
! ATTTTTCCAACCGTGGCTAA+TGG + chr7_4:44884053-44884072 None:intergenic 40.0%
!!! TGAAGTCATTTTTCCAACCG+TGG + chr7_4:44884060-44884079 None:intergenic 40.0%
ACGGTTTCCTTGTGCTTATT+TGG + chr7_4:44884116-44884135 None:intergenic 40.0%
!!! GCTCAGGCTTTTTGGTTTTA+TGG - chr7_4:44884295-44884314 Msa1156560:intron 40.0%
TAAACACAACACTCTGCACA+CGG + chr7_4:44884370-44884389 None:intergenic 40.0%
AAGACAACCATTCCGAGTAT+TGG + chr7_4:44884452-44884471 None:intergenic 40.0%
ATACTCGGAATGGTTGTCTT+TGG - chr7_4:44884452-44884471 Msa1156560:intron 40.0%
TACTCGGAATGGTTGTCTTT+GGG - chr7_4:44884453-44884472 Msa1156560:intron 40.0%
AAAGCAGTACTACCAAGGAA+AGG + chr7_4:44884584-44884603 None:intergenic 40.0%
TTTGTGGTACGATACGAGAA+TGG - chr7_4:44884653-44884672 Msa1156560:five_prime_UTR 40.0%
CATATGGAATAAACCAACGG+TGG + chr7_4:44884691-44884710 None:intergenic 40.0%
TCCATATGCACCATGATTTC+CGG - chr7_4:44884703-44884722 Msa1156560:five_prime_UTR 40.0%
!! TAAAAAGTCAAAGTGCTGGC+CGG + chr7_4:44884725-44884744 None:intergenic 40.0%
CGACACATCAAATGACCACA+TGG + chr7_4:44882862-44882881 None:intergenic 45.0%
AAGGACATGCTGCAATTTGG+TGG - chr7_4:44883458-44883477 Msa1156560:CDS 45.0%
GAACCAAGAAATCCTCTTGG+TGG - chr7_4:44883487-44883506 Msa1156560:CDS 45.0%
CATCCACCAAGAGGATTTCT+TGG + chr7_4:44883493-44883512 None:intergenic 45.0%
AGTTAGAAGGAGGATCAGAG+AGG + chr7_4:44883871-44883890 None:intergenic 45.0%
GATCCACGTGTCGAAGAAAA+GGG + chr7_4:44884228-44884247 None:intergenic 45.0%
ACCACGTTGGACCTTCATAA+GGG + chr7_4:44884258-44884277 None:intergenic 45.0%
AGGTCCAACGTGGTCAAATA+TGG - chr7_4:44884264-44884283 Msa1156560:intron 45.0%
GGTCCAACGTGGTCAAATAT+GGG - chr7_4:44884265-44884284 Msa1156560:intron 45.0%
AGACCCATATTTGACCACGT+TGG + chr7_4:44884271-44884290 None:intergenic 45.0%
AAAACCAAAAAGCCTGAGCG+AGG + chr7_4:44884294-44884313 None:intergenic 45.0%
GTGTGTTCCAATACTCGGAA+TGG - chr7_4:44884442-44884461 Msa1156560:intron 45.0%
ACTCGGAATGGTTGTCTTTG+GGG - chr7_4:44884454-44884473 Msa1156560:intron 45.0%
TACGCAAAGCAGTACTACCA+AGG + chr7_4:44884589-44884608 None:intergenic 45.0%
ACTGCTTTGCGTATTGCTGT+TGG - chr7_4:44884596-44884615 Msa1156560:five_prime_UTR 45.0%
! CTGCTTTGCGTATTGCTGTT+GGG - chr7_4:44884597-44884616 Msa1156560:five_prime_UTR 45.0%
! TTGCTGTTGGGCACTAAACT+TGG - chr7_4:44884609-44884628 Msa1156560:five_prime_UTR 45.0%
CGACACATCAAATGACCACA+TGG + chr7_4:44882862-44882881 None:intergenic 45.0%
AAGGACATGCTGCAATTTGG+TGG - chr7_4:44883458-44883477 Msa1156560:CDS 45.0%
GAACCAAGAAATCCTCTTGG+TGG - chr7_4:44883487-44883506 Msa1156560:CDS 45.0%
CATCCACCAAGAGGATTTCT+TGG + chr7_4:44883493-44883512 None:intergenic 45.0%
AGTTAGAAGGAGGATCAGAG+AGG + chr7_4:44883871-44883890 None:intergenic 45.0%
GATCCACGTGTCGAAGAAAA+GGG + chr7_4:44884228-44884247 None:intergenic 45.0%
ACCACGTTGGACCTTCATAA+GGG + chr7_4:44884258-44884277 None:intergenic 45.0%
AGGTCCAACGTGGTCAAATA+TGG - chr7_4:44884264-44884283 Msa1156560:intron 45.0%
GGTCCAACGTGGTCAAATAT+GGG - chr7_4:44884265-44884284 Msa1156560:intron 45.0%
AGACCCATATTTGACCACGT+TGG + chr7_4:44884271-44884290 None:intergenic 45.0%
AAAACCAAAAAGCCTGAGCG+AGG + chr7_4:44884294-44884313 None:intergenic 45.0%
GTGTGTTCCAATACTCGGAA+TGG - chr7_4:44884442-44884461 Msa1156560:intron 45.0%
ACTCGGAATGGTTGTCTTTG+GGG - chr7_4:44884454-44884473 Msa1156560:intron 45.0%
TACGCAAAGCAGTACTACCA+AGG + chr7_4:44884589-44884608 None:intergenic 45.0%
ACTGCTTTGCGTATTGCTGT+TGG - chr7_4:44884596-44884615 Msa1156560:five_prime_UTR 45.0%
! CTGCTTTGCGTATTGCTGTT+GGG - chr7_4:44884597-44884616 Msa1156560:five_prime_UTR 45.0%
! TTGCTGTTGGGCACTAAACT+TGG - chr7_4:44884609-44884628 Msa1156560:five_prime_UTR 45.0%
ACACAACCATTAGCCACGGT+TGG - chr7_4:44884044-44884063 Msa1156560:CDS 50.0%
! GTTCCCTTTTCTTCGACACG+TGG - chr7_4:44884222-44884241 Msa1156560:intron 50.0%
CGATCCACGTGTCGAAGAAA+AGG + chr7_4:44884229-44884248 None:intergenic 50.0%
GATCGAGCCATCCCTTATGA+AGG - chr7_4:44884244-44884263 Msa1156560:intron 50.0%
CGTTGGACCTTCATAAGGGA+TGG + chr7_4:44884254-44884273 None:intergenic 50.0%
TCCCTTATGAAGGTCCAACG+TGG - chr7_4:44884254-44884273 Msa1156560:intron 50.0%
GACCACGTTGGACCTTCATA+AGG + chr7_4:44884259-44884278 None:intergenic 50.0%
AAATATGGGTCTCCTCGCTC+AGG - chr7_4:44884279-44884298 Msa1156560:intron 50.0%
GCCGGAAATCATGGTGCATA+TGG + chr7_4:44884707-44884726 None:intergenic 50.0%
!! AAAGTGCTGGCCGGAAATCA+TGG + chr7_4:44884716-44884735 None:intergenic 50.0%
ACACAACCATTAGCCACGGT+TGG - chr7_4:44884044-44884063 Msa1156560:CDS 50.0%
! GTTCCCTTTTCTTCGACACG+TGG - chr7_4:44884222-44884241 Msa1156560:intron 50.0%
CGATCCACGTGTCGAAGAAA+AGG + chr7_4:44884229-44884248 None:intergenic 50.0%
GATCGAGCCATCCCTTATGA+AGG - chr7_4:44884244-44884263 Msa1156560:intron 50.0%
CGTTGGACCTTCATAAGGGA+TGG + chr7_4:44884254-44884273 None:intergenic 50.0%
TCCCTTATGAAGGTCCAACG+TGG - chr7_4:44884254-44884273 Msa1156560:intron 50.0%
GACCACGTTGGACCTTCATA+AGG + chr7_4:44884259-44884278 None:intergenic 50.0%
AAATATGGGTCTCCTCGCTC+AGG - chr7_4:44884279-44884298 Msa1156560:intron 50.0%
GCCGGAAATCATGGTGCATA+TGG + chr7_4:44884707-44884726 None:intergenic 50.0%
!! AAAGTGCTGGCCGGAAATCA+TGG + chr7_4:44884716-44884735 None:intergenic 50.0%
! GTCTCCTCGCTCAGGCTTTT+TGG - chr7_4:44884287-44884306 Msa1156560:intron 55.0%
ACACTCTGCACACGGACGAA+TGG + chr7_4:44884362-44884381 None:intergenic 55.0%
!! GTCTTTGGGGTGTTTGCTCG+TGG - chr7_4:44884467-44884486 Msa1156560:intron 55.0%
GACGAATGCATCACCACCGT+TGG - chr7_4:44884675-44884694 Msa1156560:five_prime_UTR 55.0%
! GTCTCCTCGCTCAGGCTTTT+TGG - chr7_4:44884287-44884306 Msa1156560:intron 55.0%
ACACTCTGCACACGGACGAA+TGG + chr7_4:44884362-44884381 None:intergenic 55.0%
!! GTCTTTGGGGTGTTTGCTCG+TGG - chr7_4:44884467-44884486 Msa1156560:intron 55.0%
GACGAATGCATCACCACCGT+TGG - chr7_4:44884675-44884694 Msa1156560:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 44882817 44884771 44882817 ID=Msa1156560;Name=Msa1156560
chr7_4 mRNA 44882817 44884771 44882817 ID=Msa1156560-mRNA-1;Parent=Msa1156560;Name=Msa1156560-mRNA-1;_AED=0.10;_eAED=0.10;_QI=277|1|1|1|0.5|0.66|3|272|300
chr7_4 exon 44882817 44883682 44882817 ID=Msa1156560-mRNA-1:exon:11641;Parent=Msa1156560-mRNA-1
chr7_4 exon 44883873 44884196 44883873 ID=Msa1156560-mRNA-1:exon:11640;Parent=Msa1156560-mRNA-1
chr7_4 exon 44884510 44884771 44884510 ID=Msa1156560-mRNA-1:exon:11639;Parent=Msa1156560-mRNA-1
chr7_4 five_prime_UTR 44884510 44884771 44884510 ID=Msa1156560-mRNA-1:five_prime_utr;Parent=Msa1156560-mRNA-1
chr7_4 five_prime_UTR 44884182 44884196 44884182 ID=Msa1156560-mRNA-1:five_prime_utr;Parent=Msa1156560-mRNA-1
chr7_4 CDS 44883873 44884181 44883873 ID=Msa1156560-mRNA-1:cds;Parent=Msa1156560-mRNA-1
chr7_4 CDS 44883089 44883682 44883089 ID=Msa1156560-mRNA-1:cds;Parent=Msa1156560-mRNA-1
chr7_4 three_prime_UTR 44882817 44883088 44882817 ID=Msa1156560-mRNA-1:three_prime_utr;Parent=Msa1156560-mRNA-1
Gene Sequence

>Msa1156560

ATGGATATAAGGTATATGATTTATGAGATGAAAAAATTAGCTGAAGACTTCAAGGACATGCTGCAATTTGGTGGTTCTGAGAACCAAGAAATCCTCTTGGTGGATGAACTTGAAAATTCAATGCGCAAATCTGTTGATGTTTTGAAGAAACAAATCATCGTGTTGGATGAACTACGACAAAATCAAGAGTTGATCAGAAGGAAATTGATTGATGATGAAATTTTCGATAGCGATGATTATATGTTTACGATTTCACGCTTAGGAAATCTTAAAGCCATTCATTATGGTTTGAAGCCTTCCAATATCCAGAATGTTCCCTTACATACTTTTATAGAGTATCCAACAAATCATAGTAAGTTCCTTATTTGTGTTGATAAAGAGGAGCAAATAAAGAGCACTGCATGGAATTTACACAAAAATGAACCGTGTGTTTTTTACATTGAATTTACACAACCATTAGCCACGGTTGGAAAAATGACTTCAATATTGCAACACAACTCCACTAGTAGTAATAATCCAAATAAGCACAAGGAAACCGTGCCAATATCATATCCTAAATTTCAGCACAAACGTAAGGAAATTGCACAATATGTTTCATTTGTTGTAATCTTGACCATTATATTTGGTTCCCTTTTCTTCGACACGTGGATCGAGCCATCCCTTATGAAGGTCCAACGTGGTCAAATATGGGTCTCCTCGCTCAGGCTTTTTGGTTTTATGGCATTTGTTTGGGCCGTTGCTATAATGATTCTTGTTGGTGTACCATTCGTCCGTGTGCAGAGTGTTGTGTTTATCTTCTGGGCTATTTTGATGCATCTTAAATCGCACAACTCAATCACTACAATGTGTGTTCCAATACTCGGAATGGTTGTCTTTGGGGTGTTTGCTCGTGGAAAATTTTAG
Protein sequence

>Msa1156560

MDIRYMIYEMKKLAEDFKDMLQFGGSENQEILLVDELENSMRKSVDVLKKQIIVLDELRQNQELIRRKLIDDEIFDSDDYMFTISRLGNLKAIHYGLKPSNIQNVPLHTFIEYPTNHSKFLICVDKEEQIKSTAWNLHKNEPCVFYIEFTQPLATVGKMTSILQHNSTSSNNPNKHKETVPISYPKFQHKRKEIAQYVSFVVILTIIFGSLFFDTWIEPSLMKVQRGQIWVSSLRLFGFMAFVWAVAIMILVGVPFVRVQSVVFIFWAILMHLKSHNSITTMCVPILGMVVFGVFARGKF*