AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1068570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1068570 MtrunA17_Chr7g0250141 61.238 307 90 7 39 323 22 321 5.58e-110 322
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAATTTCTTTGTTTAATT+TGG 0.081323 7_2:+63595653 None:intergenic
TATTTCTTGTGACAACTTAT+TGG 0.139479 7_2:-63595680 Msa1068570:three_prime_UTR
TTACGTTTGTGCTGAAATTT+AGG 0.171556 7_2:+63596298 None:intergenic
CAAATTAAACAAAGAAATTT+AGG 0.210574 7_2:-63595652 Msa1068570:three_prime_UTR
ACGGTTTCCTTGTGCTTATT+TGG 0.219708 7_2:+63596334 None:intergenic
GTAATCTTGACCATTATATT+TGG 0.230664 7_2:-63596247 Msa1068570:CDS
TCCACATGCACCATGATTTC+CGG 0.235500 7_2:-63595744 Msa1068570:three_prime_UTR
AGGTCCAACGTGGTCAAATA+TGG 0.249406 7_2:-63596183 Msa1068570:CDS
AATCTTAAACCCATTCATTA+TGG 0.260718 7_2:-63596773 Msa1068570:CDS
CATCCAACAAGAGGATTTCT+TGG 0.273651 7_2:+63596975 None:intergenic
TACTCGGAATAGTTGTCTTT+GGG 0.278337 7_2:-63595994 Msa1068570:CDS
GAGTGTTGTGTTTATCTTCT+GGG 0.288933 7_2:-63596071 Msa1068570:CDS
CCTCTTTATCAACACAAATA+AGG 0.303589 7_2:+63596491 None:intergenic
ATACTCGGAATAGTTGTCTT+TGG 0.322656 7_2:-63595995 Msa1068570:CDS
TAGCTTCGTTCAAGATTGTT+AGG 0.328816 7_2:-63597407 Msa1068570:intron
TTCAAGTACATGCTGCAATT+TGG 0.337988 7_2:-63597010 Msa1068570:CDS
AGAGTGTTGTGTTTATCTTC+TGG 0.340356 7_2:-63596072 Msa1068570:CDS
TGAGCAACTTACCTGGATAT+TGG 0.346014 7_2:+63596739 None:intergenic
CGATCCACGTGTCGAAGAAA+AGG 0.367683 7_2:+63596221 None:intergenic
GAAAAGGGAACCAAATATAA+TGG 0.381996 7_2:+63596237 None:intergenic
AATATTTACGATTTATGAGT+TGG 0.387542 7_2:-63597050 Msa1068570:CDS
GAACCAAGAAATCCTCTTGT+TGG 0.390830 7_2:-63596978 Msa1068570:CDS
CTGCTTTGCGTATTGCTGTT+GGG 0.401892 7_2:-63595850 Msa1068570:three_prime_UTR
ATACTCTATAAAAGTATGTA+AGG 0.405060 7_2:+63596534 None:intergenic
AGGAACTTACTATGATTTGT+TGG 0.419337 7_2:+63596511 None:intergenic
CCTTATTTGTGTTGATAAAG+AGG 0.421351 7_2:-63596491 Msa1068570:CDS
TGAAATTTAGGATATGATAT+TGG 0.422942 7_2:+63596310 None:intergenic
TAATGTAATATACCTTTCAT+TGG 0.426452 7_2:-63595878 Msa1068570:three_prime_UTR
TAATTAAGACAAATGCTTTG+TGG 0.431922 7_2:-63595810 Msa1068570:three_prime_UTR
GACCACGTTGGACCTTCATA+AGG 0.436592 7_2:+63596191 None:intergenic
ATGTGAACCTGAGATATTAC+AGG 0.439567 7_2:+63597578 None:intergenic
GCTGCTTTGCGTATTGCTGT+TGG 0.444175 7_2:-63595851 Msa1068570:three_prime_UTR
ACCACGTTGGACCTTCATAA+GGG 0.454092 7_2:+63596192 None:intergenic
TTGCTGTTGGGCACTAAACT+TGG 0.457266 7_2:-63595838 Msa1068570:three_prime_UTR
GGCTTCAAACCATAATGAAT+GGG 0.460583 7_2:+63596764 None:intergenic
AGGCTTCAAACCATAATGAA+TGG 0.471617 7_2:+63596763 None:intergenic
AGATTCTAAATATATTTGTC+AGG 0.473876 7_2:+63597497 None:intergenic
AATCTTAGAATGTTGATTTG+AGG 0.478486 7_2:+63597463 None:intergenic
ATGTTTAAGATTTCACGCTT+AGG 0.481504 7_2:-63596797 Msa1068570:CDS
TCGAAATCCTGTAATATCTC+AGG 0.486803 7_2:-63597585 Msa1068570:five_prime_UTR
GTTGCTATAATGATTCTTGT+TGG 0.493716 7_2:-63596115 Msa1068570:CDS
TGTGCAAATCTGAAAATCTT+CGG 0.496767 7_2:-63596937 Msa1068570:CDS
TTTGAAGCCTTCCAATATCC+AGG 0.501125 7_2:-63596750 Msa1068570:intron
AAGTACATGCTGCAATTTGG+TGG 0.504000 7_2:-63597007 Msa1068570:CDS
AAAGTGCTGGCCGGAAATCA+TGG 0.508063 7_2:+63595734 None:intergenic
ACACAACCATTAGCCAAGGT+TGG 0.509145 7_2:-63596403 Msa1068570:CDS
AAATATGGGTCTCCTCGCTC+AGG 0.509899 7_2:-63596168 Msa1068570:CDS
GATCCACGTGTCGAAGAAAA+GGG 0.523570 7_2:+63596222 None:intergenic
GGTCCAACGTGGTCAAATAT+GGG 0.526486 7_2:-63596182 Msa1068570:CDS
TTTCAAGTTCATCCAACAAG+AGG 0.532536 7_2:+63596966 None:intergenic
GACGAATGCATCACCACCGT+TGG 0.543227 7_2:-63595772 Msa1068570:three_prime_UTR
TAAATTTCAGCACAAACGTA+AGG 0.550229 7_2:-63596296 Msa1068570:CDS
ATTTGGATTATTACTACTAG+TGG 0.555390 7_2:+63596351 None:intergenic
AGCAAATAAAGAGCACTGCA+TGG 0.561104 7_2:-63596468 Msa1068570:CDS
CAACTTACCTGGATATTGGA+AGG 0.568102 7_2:+63596743 None:intergenic
AAAATCAAGAGTTGATCAGA+AGG 0.572726 7_2:-63596859 Msa1068570:CDS
ATGTTGATTTGAGGATGAAA+CGG 0.585785 7_2:+63597472 None:intergenic
GATCGAGCCATCCCTTATGA+AGG 0.587003 7_2:-63596203 Msa1068570:CDS
ACTCGGAATAGTTGTCTTTG+GGG 0.587329 7_2:-63595993 Msa1068570:CDS
ACACTCTGCACACTGACGAA+TGG 0.594198 7_2:+63596088 None:intergenic
AACAAGAATCATTATAGCAA+CGG 0.594551 7_2:+63596117 None:intergenic
TTTGTGGTACGATACGAGAA+TGG 0.597174 7_2:-63595794 Msa1068570:three_prime_UTR
CGTTGGACCTTCATAAGGGA+TGG 0.600614 7_2:+63596196 None:intergenic
GCCGGAAATCATGGTGCATG+TGG 0.601199 7_2:+63595743 None:intergenic
GCAATGCGTGTTCAAATACT+CGG 0.602116 7_2:-63596010 Msa1068570:CDS
ATTTACACAACCATTAGCCA+AGG 0.606338 7_2:-63596407 Msa1068570:CDS
TACTCTATAAAAGTATGTAA+GGG 0.610014 7_2:+63596535 None:intergenic
TTACTTTGAATCAGTCCATG+TGG 0.639089 7_2:-63597620 Msa1068570:five_prime_UTR
AAAGCAGCACTACCAATGAA+AGG 0.642670 7_2:+63595866 None:intergenic
CGACACATCAAATGACCACA+TGG 0.645456 7_2:+63597605 None:intergenic
TTTAGGATATGATATTGGCA+CGG 0.659698 7_2:+63596315 None:intergenic
AGACCCATATTTGACCACGT+TGG 0.670283 7_2:+63596179 None:intergenic
TAATAATCCAAATAAGCACA+AGG 0.676862 7_2:-63596341 Msa1068570:CDS
GTGCATGTGGAATAAACCAA+CGG 0.721729 7_2:+63595756 None:intergenic
TCCCTTATGAAGGTCCAACG+TGG 0.728134 7_2:-63596193 Msa1068570:CDS
CATGTGGAATAAACCAACGG+TGG 0.797614 7_2:+63595759 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAATCAAAACATTTCA+AGG + chr7_2:63595928-63595947 None:intergenic 15.0%
!!! CTAAATTTCTTTGTTTAATT+TGG + chr7_2:63597583-63597602 None:intergenic 15.0%
!! CAAATTAAACAAAGAAATTT+AGG - chr7_2:63597581-63597600 Msa1068570:five_prime_UTR 15.0%
!! TATTAAATCAAAACATTTCA+AGG + chr7_2:63595928-63595947 None:intergenic 15.0%
!!! CTAAATTTCTTTGTTTAATT+TGG + chr7_2:63597583-63597602 None:intergenic 15.0%
!! CAAATTAAACAAAGAAATTT+AGG - chr7_2:63597581-63597600 Msa1068570:five_prime_UTR 15.0%
!! AAAAAGATGAAACAGATTAT+GGG + chr7_2:63595687-63595706 None:intergenic 20.0%
!! TAAAAAGATGAAACAGATTA+TGG + chr7_2:63595688-63595707 None:intergenic 20.0%
!! AGATTCTAAATATATTTGTC+AGG + chr7_2:63595739-63595758 None:intergenic 20.0%
!! CCAGAAAGAAAAAAAAAAAT+TGG + chr7_2:63595896-63595915 None:intergenic 20.0%
!!! CCAATTTTTTTTTTTCTTTC+TGG - chr7_2:63595893-63595912 Msa1068570:three_prime_UTR 20.0%
!! AATATTTACGATTTATGAGT+TGG - chr7_2:63596183-63596202 Msa1068570:CDS 20.0%
!! CTAAGAATAATAGTAAAAGT+CGG + chr7_2:63596575-63596594 None:intergenic 20.0%
!!! TACTCTATAAAAGTATGTAA+GGG + chr7_2:63596701-63596720 None:intergenic 20.0%
!!! ATACTCTATAAAAGTATGTA+AGG + chr7_2:63596702-63596721 None:intergenic 20.0%
!! AATTCAATGTAAAAAACACA+CGG + chr7_2:63596809-63596828 None:intergenic 20.0%
!!! TGAAATTTAGGATATGATAT+TGG + chr7_2:63596926-63596945 None:intergenic 20.0%
!!! TGGAAAATTTTAGAGAATAT+TGG - chr7_2:63597273-63597292 Msa1068570:intron 20.0%
!! TAATGTAATATACCTTTCAT+TGG - chr7_2:63597355-63597374 Msa1068570:intron 20.0%
!! AAAAAGATGAAACAGATTAT+GGG + chr7_2:63595687-63595706 None:intergenic 20.0%
!! TAAAAAGATGAAACAGATTA+TGG + chr7_2:63595688-63595707 None:intergenic 20.0%
!! AGATTCTAAATATATTTGTC+AGG + chr7_2:63595739-63595758 None:intergenic 20.0%
!! CCAGAAAGAAAAAAAAAAAT+TGG + chr7_2:63595896-63595915 None:intergenic 20.0%
!!! CCAATTTTTTTTTTTCTTTC+TGG - chr7_2:63595893-63595912 Msa1068570:three_prime_UTR 20.0%
!! AATATTTACGATTTATGAGT+TGG - chr7_2:63596183-63596202 Msa1068570:CDS 20.0%
!! CTAAGAATAATAGTAAAAGT+CGG + chr7_2:63596575-63596594 None:intergenic 20.0%
!!! TACTCTATAAAAGTATGTAA+GGG + chr7_2:63596701-63596720 None:intergenic 20.0%
!!! ATACTCTATAAAAGTATGTA+AGG + chr7_2:63596702-63596721 None:intergenic 20.0%
!! AATTCAATGTAAAAAACACA+CGG + chr7_2:63596809-63596828 None:intergenic 20.0%
!!! TGAAATTTAGGATATGATAT+TGG + chr7_2:63596926-63596945 None:intergenic 20.0%
!!! TGGAAAATTTTAGAGAATAT+TGG - chr7_2:63597273-63597292 Msa1068570:intron 20.0%
!! TAATGTAATATACCTTTCAT+TGG - chr7_2:63597355-63597374 Msa1068570:intron 20.0%
!! ATAACTTTTACTGTGGAATT+TGG + chr7_2:63595716-63595735 None:intergenic 25.0%
!!! AATCTTAGAATGTTGATTTG+AGG + chr7_2:63595773-63595792 None:intergenic 25.0%
! AAAAAATTGGACAAGAGAAT+AGG + chr7_2:63595883-63595902 None:intergenic 25.0%
! TTCATAAACCCTAACAATAT+AGG - chr7_2:63596014-63596033 Msa1068570:CDS 25.0%
!!! ATTTCTTTTTCATGGATAAC+AGG - chr7_2:63596137-63596156 Msa1068570:CDS 25.0%
!!! TTTCTTTTTCATGGATAACA+GGG - chr7_2:63596138-63596157 Msa1068570:CDS 25.0%
! AATCTTAAACCCATTCATTA+TGG - chr7_2:63596460-63596479 Msa1068570:CDS 25.0%
! TAAAAGTCGGTCAAAAAAAA+TGG + chr7_2:63596562-63596581 None:intergenic 25.0%
! TTAGAAAGACAGATATTACA+AGG - chr7_2:63596591-63596610 Msa1068570:intron 25.0%
! AAAAAAATCAAAATGAGTCC+TGG + chr7_2:63596621-63596640 None:intergenic 25.0%
! ATTTGGATTATTACTACTAG+TGG + chr7_2:63596885-63596904 None:intergenic 25.0%
! TAATAATCCAAATAAGCACA+AGG - chr7_2:63596892-63596911 Msa1068570:CDS 25.0%
! GTAATCTTGACCATTATATT+TGG - chr7_2:63596986-63597005 Msa1068570:CDS 25.0%
! AACAAGAATCATTATAGCAA+CGG + chr7_2:63597119-63597138 None:intergenic 25.0%
! TAATTAAGACAAATGCTTTG+TGG - chr7_2:63597423-63597442 Msa1068570:five_prime_UTR 25.0%
! TATTTCTTGTGACAACTTAT+TGG - chr7_2:63597553-63597572 Msa1068570:five_prime_UTR 25.0%
!! ATAACTTTTACTGTGGAATT+TGG + chr7_2:63595716-63595735 None:intergenic 25.0%
!!! AATCTTAGAATGTTGATTTG+AGG + chr7_2:63595773-63595792 None:intergenic 25.0%
! AAAAAATTGGACAAGAGAAT+AGG + chr7_2:63595883-63595902 None:intergenic 25.0%
! TTCATAAACCCTAACAATAT+AGG - chr7_2:63596014-63596033 Msa1068570:CDS 25.0%
!!! ATTTCTTTTTCATGGATAAC+AGG - chr7_2:63596137-63596156 Msa1068570:CDS 25.0%
!!! TTTCTTTTTCATGGATAACA+GGG - chr7_2:63596138-63596157 Msa1068570:CDS 25.0%
! AATCTTAAACCCATTCATTA+TGG - chr7_2:63596460-63596479 Msa1068570:CDS 25.0%
! TAAAAGTCGGTCAAAAAAAA+TGG + chr7_2:63596562-63596581 None:intergenic 25.0%
! TTAGAAAGACAGATATTACA+AGG - chr7_2:63596591-63596610 Msa1068570:intron 25.0%
! AAAAAAATCAAAATGAGTCC+TGG + chr7_2:63596621-63596640 None:intergenic 25.0%
! ATTTGGATTATTACTACTAG+TGG + chr7_2:63596885-63596904 None:intergenic 25.0%
! TAATAATCCAAATAAGCACA+AGG - chr7_2:63596892-63596911 Msa1068570:CDS 25.0%
! GTAATCTTGACCATTATATT+TGG - chr7_2:63596986-63597005 Msa1068570:CDS 25.0%
! AACAAGAATCATTATAGCAA+CGG + chr7_2:63597119-63597138 None:intergenic 25.0%
! TAATTAAGACAAATGCTTTG+TGG - chr7_2:63597423-63597442 Msa1068570:five_prime_UTR 25.0%
! TATTTCTTGTGACAACTTAT+TGG - chr7_2:63597553-63597572 Msa1068570:five_prime_UTR 25.0%
!! ATGTTGATTTGAGGATGAAA+CGG + chr7_2:63595764-63595783 None:intergenic 30.0%
AAGAGAATAGGAAATTGGAA+TGG + chr7_2:63595871-63595890 None:intergenic 30.0%
TGCTACATCCTATATTGTTA+GGG + chr7_2:63596025-63596044 None:intergenic 30.0%
ATGCTACATCCTATATTGTT+AGG + chr7_2:63596026-63596045 None:intergenic 30.0%
GATGTAGCATAATATAGCTT+AGG - chr7_2:63596036-63596055 Msa1068570:CDS 30.0%
!! GTGTACACATTTCTTTTTCA+TGG - chr7_2:63596129-63596148 Msa1068570:CDS 30.0%
!! TTCTTTTTCATGGATAACAG+GGG - chr7_2:63596139-63596158 Msa1068570:CDS 30.0%
TGTGCAAATCTGAAAATCTT+CGG - chr7_2:63596296-63596315 Msa1068570:CDS 30.0%
! TGAAGAAACAAATTTTCACG+TGG - chr7_2:63596338-63596357 Msa1068570:CDS 30.0%
! GAAGAAACAAATTTTCACGT+GGG - chr7_2:63596339-63596358 Msa1068570:CDS 30.0%
! AAAATCAAGAGTTGATCAGA+AGG - chr7_2:63596374-63596393 Msa1068570:CDS 30.0%
! ATGTTTAAGATTTCACGCTT+AGG - chr7_2:63596436-63596455 Msa1068570:CDS 30.0%
TCGGTCAAAAAAAATGGTAA+TGG + chr7_2:63596556-63596575 None:intergenic 30.0%
! AGGAACTTACTATGATTTGT+TGG + chr7_2:63596725-63596744 None:intergenic 30.0%
CCTCTTTATCAACACAAATA+AGG + chr7_2:63596745-63596764 None:intergenic 30.0%
! CCTTATTTGTGTTGATAAAG+AGG - chr7_2:63596742-63596761 Msa1068570:intron 30.0%
! TTTAGGATATGATATTGGCA+CGG + chr7_2:63596921-63596940 None:intergenic 30.0%
TTACGTTTGTGCTGAAATTT+AGG + chr7_2:63596938-63596957 None:intergenic 30.0%
TAAATTTCAGCACAAACGTA+AGG - chr7_2:63596937-63596956 Msa1068570:CDS 30.0%
GAAAAGGGAACCAAATATAA+TGG + chr7_2:63596999-63597018 None:intergenic 30.0%
GTTGCTATAATGATTCTTGT+TGG - chr7_2:63597118-63597137 Msa1068570:exon 30.0%
!! ATGTTGATTTGAGGATGAAA+CGG + chr7_2:63595764-63595783 None:intergenic 30.0%
AAGAGAATAGGAAATTGGAA+TGG + chr7_2:63595871-63595890 None:intergenic 30.0%
TGCTACATCCTATATTGTTA+GGG + chr7_2:63596025-63596044 None:intergenic 30.0%
ATGCTACATCCTATATTGTT+AGG + chr7_2:63596026-63596045 None:intergenic 30.0%
GATGTAGCATAATATAGCTT+AGG - chr7_2:63596036-63596055 Msa1068570:CDS 30.0%
!! GTGTACACATTTCTTTTTCA+TGG - chr7_2:63596129-63596148 Msa1068570:CDS 30.0%
!! TTCTTTTTCATGGATAACAG+GGG - chr7_2:63596139-63596158 Msa1068570:CDS 30.0%
TGTGCAAATCTGAAAATCTT+CGG - chr7_2:63596296-63596315 Msa1068570:CDS 30.0%
! TGAAGAAACAAATTTTCACG+TGG - chr7_2:63596338-63596357 Msa1068570:CDS 30.0%
! GAAGAAACAAATTTTCACGT+GGG - chr7_2:63596339-63596358 Msa1068570:CDS 30.0%
! AAAATCAAGAGTTGATCAGA+AGG - chr7_2:63596374-63596393 Msa1068570:CDS 30.0%
! ATGTTTAAGATTTCACGCTT+AGG - chr7_2:63596436-63596455 Msa1068570:CDS 30.0%
TCGGTCAAAAAAAATGGTAA+TGG + chr7_2:63596556-63596575 None:intergenic 30.0%
! AGGAACTTACTATGATTTGT+TGG + chr7_2:63596725-63596744 None:intergenic 30.0%
CCTCTTTATCAACACAAATA+AGG + chr7_2:63596745-63596764 None:intergenic 30.0%
! CCTTATTTGTGTTGATAAAG+AGG - chr7_2:63596742-63596761 Msa1068570:intron 30.0%
! TTTAGGATATGATATTGGCA+CGG + chr7_2:63596921-63596940 None:intergenic 30.0%
TTACGTTTGTGCTGAAATTT+AGG + chr7_2:63596938-63596957 None:intergenic 30.0%
TAAATTTCAGCACAAACGTA+AGG - chr7_2:63596937-63596956 Msa1068570:CDS 30.0%
GAAAAGGGAACCAAATATAA+TGG + chr7_2:63596999-63597018 None:intergenic 30.0%
GTTGCTATAATGATTCTTGT+TGG - chr7_2:63597118-63597137 Msa1068570:exon 30.0%
TCGAAATCCTGTAATATCTC+AGG - chr7_2:63595648-63595667 Msa1068570:three_prime_UTR 35.0%
ATGTGAACCTGAGATATTAC+AGG + chr7_2:63595658-63595677 None:intergenic 35.0%
! TGTCAGGATAACTTTTACTG+TGG + chr7_2:63595723-63595742 None:intergenic 35.0%
TAGCTTCGTTCAAGATTGTT+AGG - chr7_2:63595826-63595845 Msa1068570:three_prime_UTR 35.0%
TGGACAAGAGAATAGGAAAT+TGG + chr7_2:63595876-63595895 None:intergenic 35.0%
!! TCTTTTTCATGGATAACAGG+GGG - chr7_2:63596140-63596159 Msa1068570:CDS 35.0%
TTCAAGTACATGCTGCAATT+TGG - chr7_2:63596223-63596242 Msa1068570:CDS 35.0%
!! TTTCAAGTTCATCCAACAAG+AGG + chr7_2:63596270-63596289 None:intergenic 35.0%
GGCTTCAAACCATAATGAAT+GGG + chr7_2:63596472-63596491 None:intergenic 35.0%
AGGCTTCAAACCATAATGAA+TGG + chr7_2:63596473-63596492 None:intergenic 35.0%
! AATAAGTTGAGCAACTTACC+TGG + chr7_2:63596504-63596523 None:intergenic 35.0%
CAGATATTACAAGGAAATCC+AGG - chr7_2:63596600-63596619 Msa1068570:intron 35.0%
GTGTATAGAAAGTTAGAAGG+AGG + chr7_2:63596667-63596686 None:intergenic 35.0%
GCAGTGTATAGAAAGTTAGA+AGG + chr7_2:63596670-63596689 None:intergenic 35.0%
ATTTACACAACCATTAGCCA+AGG - chr7_2:63596826-63596845 Msa1068570:CDS 35.0%
! ATTTTTCCAACCTTGGCTAA+TGG + chr7_2:63596839-63596858 None:intergenic 35.0%
!!! TGAAGTCATTTTTCCAACCT+TGG + chr7_2:63596846-63596865 None:intergenic 35.0%
!!! TTGGTTTTATGGCGTTTGTT+TGG - chr7_2:63597092-63597111 Msa1068570:CDS 35.0%
!!! TGGTTTTATGGCGTTTGTTT+GGG - chr7_2:63597093-63597112 Msa1068570:CDS 35.0%
!! AGAGTGTTGTGTTTATCTTC+TGG - chr7_2:63597161-63597180 Msa1068570:intron 35.0%
! GAGTGTTGTGTTTATCTTCT+GGG - chr7_2:63597162-63597181 Msa1068570:intron 35.0%
ATACTCGGAATAGTTGTCTT+TGG - chr7_2:63597238-63597257 Msa1068570:intron 35.0%
TACTCGGAATAGTTGTCTTT+GGG - chr7_2:63597239-63597258 Msa1068570:intron 35.0%
GCTATAAAAAGTCAAAGTGC+TGG + chr7_2:63597515-63597534 None:intergenic 35.0%
TCGAAATCCTGTAATATCTC+AGG - chr7_2:63595648-63595667 Msa1068570:three_prime_UTR 35.0%
ATGTGAACCTGAGATATTAC+AGG + chr7_2:63595658-63595677 None:intergenic 35.0%
! TGTCAGGATAACTTTTACTG+TGG + chr7_2:63595723-63595742 None:intergenic 35.0%
TAGCTTCGTTCAAGATTGTT+AGG - chr7_2:63595826-63595845 Msa1068570:three_prime_UTR 35.0%
TGGACAAGAGAATAGGAAAT+TGG + chr7_2:63595876-63595895 None:intergenic 35.0%
!! TCTTTTTCATGGATAACAGG+GGG - chr7_2:63596140-63596159 Msa1068570:CDS 35.0%
TTCAAGTACATGCTGCAATT+TGG - chr7_2:63596223-63596242 Msa1068570:CDS 35.0%
!! TTTCAAGTTCATCCAACAAG+AGG + chr7_2:63596270-63596289 None:intergenic 35.0%
GGCTTCAAACCATAATGAAT+GGG + chr7_2:63596472-63596491 None:intergenic 35.0%
AGGCTTCAAACCATAATGAA+TGG + chr7_2:63596473-63596492 None:intergenic 35.0%
! AATAAGTTGAGCAACTTACC+TGG + chr7_2:63596504-63596523 None:intergenic 35.0%
CAGATATTACAAGGAAATCC+AGG - chr7_2:63596600-63596619 Msa1068570:intron 35.0%
GTGTATAGAAAGTTAGAAGG+AGG + chr7_2:63596667-63596686 None:intergenic 35.0%
GCAGTGTATAGAAAGTTAGA+AGG + chr7_2:63596670-63596689 None:intergenic 35.0%
ATTTACACAACCATTAGCCA+AGG - chr7_2:63596826-63596845 Msa1068570:CDS 35.0%
! ATTTTTCCAACCTTGGCTAA+TGG + chr7_2:63596839-63596858 None:intergenic 35.0%
!!! TGAAGTCATTTTTCCAACCT+TGG + chr7_2:63596846-63596865 None:intergenic 35.0%
!!! TTGGTTTTATGGCGTTTGTT+TGG - chr7_2:63597092-63597111 Msa1068570:CDS 35.0%
!!! TGGTTTTATGGCGTTTGTTT+GGG - chr7_2:63597093-63597112 Msa1068570:CDS 35.0%
!! AGAGTGTTGTGTTTATCTTC+TGG - chr7_2:63597161-63597180 Msa1068570:intron 35.0%
! GAGTGTTGTGTTTATCTTCT+GGG - chr7_2:63597162-63597181 Msa1068570:intron 35.0%
ATACTCGGAATAGTTGTCTT+TGG - chr7_2:63597238-63597257 Msa1068570:intron 35.0%
TACTCGGAATAGTTGTCTTT+GGG - chr7_2:63597239-63597258 Msa1068570:intron 35.0%
GCTATAAAAAGTCAAAGTGC+TGG + chr7_2:63597515-63597534 None:intergenic 35.0%
CAGGGGGAAATTTGATAAGA+TGG - chr7_2:63596156-63596175 Msa1068570:CDS 40.0%
AAGTACATGCTGCAATTTGG+TGG - chr7_2:63596226-63596245 Msa1068570:CDS 40.0%
GAACCAAGAAATCCTCTTGT+TGG - chr7_2:63596255-63596274 Msa1068570:CDS 40.0%
CATCCAACAAGAGGATTTCT+TGG + chr7_2:63596261-63596280 None:intergenic 40.0%
! TTTGAAGCCTTCCAATATCC+AGG - chr7_2:63596483-63596502 Msa1068570:CDS 40.0%
CAACTTACCTGGATATTGGA+AGG + chr7_2:63596493-63596512 None:intergenic 40.0%
TGAGCAACTTACCTGGATAT+TGG + chr7_2:63596497-63596516 None:intergenic 40.0%
!! AGCAAATAAAGAGCACTGCA+TGG - chr7_2:63596765-63596784 Msa1068570:CDS 40.0%
ACGGTTTCCTTGTGCTTATT+TGG + chr7_2:63596902-63596921 None:intergenic 40.0%
!!! GCTCAGGCTTTTTGGTTTTA+TGG - chr7_2:63597081-63597100 Msa1068570:CDS 40.0%
GCAATGCGTGTTCAAATACT+CGG - chr7_2:63597223-63597242 Msa1068570:intron 40.0%
ACTCGGAATAGTTGTCTTTG+GGG - chr7_2:63597240-63597259 Msa1068570:intron 40.0%
AAAGCAGCACTACCAATGAA+AGG + chr7_2:63597370-63597389 None:intergenic 40.0%
TTTGTGGTACGATACGAGAA+TGG - chr7_2:63597439-63597458 Msa1068570:five_prime_UTR 40.0%
GTGCATGTGGAATAAACCAA+CGG + chr7_2:63597480-63597499 None:intergenic 40.0%
!! TAAAAAGTCAAAGTGCTGGC+CGG + chr7_2:63597511-63597530 None:intergenic 40.0%
CAGGGGGAAATTTGATAAGA+TGG - chr7_2:63596156-63596175 Msa1068570:CDS 40.0%
AAGTACATGCTGCAATTTGG+TGG - chr7_2:63596226-63596245 Msa1068570:CDS 40.0%
GAACCAAGAAATCCTCTTGT+TGG - chr7_2:63596255-63596274 Msa1068570:CDS 40.0%
CATCCAACAAGAGGATTTCT+TGG + chr7_2:63596261-63596280 None:intergenic 40.0%
! TTTGAAGCCTTCCAATATCC+AGG - chr7_2:63596483-63596502 Msa1068570:CDS 40.0%
CAACTTACCTGGATATTGGA+AGG + chr7_2:63596493-63596512 None:intergenic 40.0%
TGAGCAACTTACCTGGATAT+TGG + chr7_2:63596497-63596516 None:intergenic 40.0%
!! AGCAAATAAAGAGCACTGCA+TGG - chr7_2:63596765-63596784 Msa1068570:CDS 40.0%
ACGGTTTCCTTGTGCTTATT+TGG + chr7_2:63596902-63596921 None:intergenic 40.0%
!!! GCTCAGGCTTTTTGGTTTTA+TGG - chr7_2:63597081-63597100 Msa1068570:CDS 40.0%
GCAATGCGTGTTCAAATACT+CGG - chr7_2:63597223-63597242 Msa1068570:intron 40.0%
ACTCGGAATAGTTGTCTTTG+GGG - chr7_2:63597240-63597259 Msa1068570:intron 40.0%
AAAGCAGCACTACCAATGAA+AGG + chr7_2:63597370-63597389 None:intergenic 40.0%
TTTGTGGTACGATACGAGAA+TGG - chr7_2:63597439-63597458 Msa1068570:five_prime_UTR 40.0%
GTGCATGTGGAATAAACCAA+CGG + chr7_2:63597480-63597499 None:intergenic 40.0%
!! TAAAAAGTCAAAGTGCTGGC+CGG + chr7_2:63597511-63597530 None:intergenic 40.0%
CGACACATCAAATGACCACA+TGG + chr7_2:63595631-63595650 None:intergenic 45.0%
AGTTAGAAGGAGGATCAGAG+AGG + chr7_2:63596657-63596676 None:intergenic 45.0%
ACACAACCATTAGCCAAGGT+TGG - chr7_2:63596830-63596849 Msa1068570:CDS 45.0%
GATCCACGTGTCGAAGAAAA+GGG + chr7_2:63597014-63597033 None:intergenic 45.0%
ACCACGTTGGACCTTCATAA+GGG + chr7_2:63597044-63597063 None:intergenic 45.0%
AGGTCCAACGTGGTCAAATA+TGG - chr7_2:63597050-63597069 Msa1068570:CDS 45.0%
GGTCCAACGTGGTCAAATAT+GGG - chr7_2:63597051-63597070 Msa1068570:CDS 45.0%
AGACCCATATTTGACCACGT+TGG + chr7_2:63597057-63597076 None:intergenic 45.0%
AAAACCAAAAAGCCTGAGCG+AGG + chr7_2:63597080-63597099 None:intergenic 45.0%
! CTGCTTTGCGTATTGCTGTT+GGG - chr7_2:63597383-63597402 Msa1068570:intron 45.0%
! TTGCTGTTGGGCACTAAACT+TGG - chr7_2:63597395-63597414 Msa1068570:intron 45.0%
CATGTGGAATAAACCAACGG+TGG + chr7_2:63597477-63597496 None:intergenic 45.0%
TCCACATGCACCATGATTTC+CGG - chr7_2:63597489-63597508 Msa1068570:five_prime_UTR 45.0%
CGACACATCAAATGACCACA+TGG + chr7_2:63595631-63595650 None:intergenic 45.0%
AGTTAGAAGGAGGATCAGAG+AGG + chr7_2:63596657-63596676 None:intergenic 45.0%
ACACAACCATTAGCCAAGGT+TGG - chr7_2:63596830-63596849 Msa1068570:CDS 45.0%
GATCCACGTGTCGAAGAAAA+GGG + chr7_2:63597014-63597033 None:intergenic 45.0%
ACCACGTTGGACCTTCATAA+GGG + chr7_2:63597044-63597063 None:intergenic 45.0%
AGGTCCAACGTGGTCAAATA+TGG - chr7_2:63597050-63597069 Msa1068570:CDS 45.0%
GGTCCAACGTGGTCAAATAT+GGG - chr7_2:63597051-63597070 Msa1068570:CDS 45.0%
AGACCCATATTTGACCACGT+TGG + chr7_2:63597057-63597076 None:intergenic 45.0%
AAAACCAAAAAGCCTGAGCG+AGG + chr7_2:63597080-63597099 None:intergenic 45.0%
! CTGCTTTGCGTATTGCTGTT+GGG - chr7_2:63597383-63597402 Msa1068570:intron 45.0%
! TTGCTGTTGGGCACTAAACT+TGG - chr7_2:63597395-63597414 Msa1068570:intron 45.0%
CATGTGGAATAAACCAACGG+TGG + chr7_2:63597477-63597496 None:intergenic 45.0%
TCCACATGCACCATGATTTC+CGG - chr7_2:63597489-63597508 Msa1068570:five_prime_UTR 45.0%
! GTTCCCTTTTCTTCGACACG+TGG - chr7_2:63597008-63597027 Msa1068570:CDS 50.0%
CGATCCACGTGTCGAAGAAA+AGG + chr7_2:63597015-63597034 None:intergenic 50.0%
GATCGAGCCATCCCTTATGA+AGG - chr7_2:63597030-63597049 Msa1068570:CDS 50.0%
CGTTGGACCTTCATAAGGGA+TGG + chr7_2:63597040-63597059 None:intergenic 50.0%
TCCCTTATGAAGGTCCAACG+TGG - chr7_2:63597040-63597059 Msa1068570:CDS 50.0%
GACCACGTTGGACCTTCATA+AGG + chr7_2:63597045-63597064 None:intergenic 50.0%
AAATATGGGTCTCCTCGCTC+AGG - chr7_2:63597065-63597084 Msa1068570:CDS 50.0%
ACACTCTGCACACTGACGAA+TGG + chr7_2:63597148-63597167 None:intergenic 50.0%
! GCTGCTTTGCGTATTGCTGT+TGG - chr7_2:63597382-63597401 Msa1068570:intron 50.0%
!! AAAGTGCTGGCCGGAAATCA+TGG + chr7_2:63597502-63597521 None:intergenic 50.0%
! GTTCCCTTTTCTTCGACACG+TGG - chr7_2:63597008-63597027 Msa1068570:CDS 50.0%
CGATCCACGTGTCGAAGAAA+AGG + chr7_2:63597015-63597034 None:intergenic 50.0%
GATCGAGCCATCCCTTATGA+AGG - chr7_2:63597030-63597049 Msa1068570:CDS 50.0%
CGTTGGACCTTCATAAGGGA+TGG + chr7_2:63597040-63597059 None:intergenic 50.0%
TCCCTTATGAAGGTCCAACG+TGG - chr7_2:63597040-63597059 Msa1068570:CDS 50.0%
GACCACGTTGGACCTTCATA+AGG + chr7_2:63597045-63597064 None:intergenic 50.0%
AAATATGGGTCTCCTCGCTC+AGG - chr7_2:63597065-63597084 Msa1068570:CDS 50.0%
ACACTCTGCACACTGACGAA+TGG + chr7_2:63597148-63597167 None:intergenic 50.0%
! GCTGCTTTGCGTATTGCTGT+TGG - chr7_2:63597382-63597401 Msa1068570:intron 50.0%
!! AAAGTGCTGGCCGGAAATCA+TGG + chr7_2:63597502-63597521 None:intergenic 50.0%
! GTCTCCTCGCTCAGGCTTTT+TGG - chr7_2:63597073-63597092 Msa1068570:CDS 55.0%
!! GTCTTTGGGGTACTTGCTCG+TGG - chr7_2:63597253-63597272 Msa1068570:intron 55.0%
GACGAATGCATCACCACCGT+TGG - chr7_2:63597461-63597480 Msa1068570:five_prime_UTR 55.0%
GCCGGAAATCATGGTGCATG+TGG + chr7_2:63597493-63597512 None:intergenic 55.0%
! GTCTCCTCGCTCAGGCTTTT+TGG - chr7_2:63597073-63597092 Msa1068570:CDS 55.0%
!! GTCTTTGGGGTACTTGCTCG+TGG - chr7_2:63597253-63597272 Msa1068570:intron 55.0%
GACGAATGCATCACCACCGT+TGG - chr7_2:63597461-63597480 Msa1068570:five_prime_UTR 55.0%
GCCGGAAATCATGGTGCATG+TGG + chr7_2:63597493-63597512 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 63595616 63597639 63595616 ID=Msa1068570;Name=Msa1068570
chr7_2 mRNA 63595616 63597639 63595616 ID=Msa1068570-mRNA-1;Parent=Msa1068570;Name=Msa1068570-mRNA-1;_AED=0.03;_eAED=0.03;_QI=264|1|1|1|0.5|0.33|3|354|323
chr7_2 exon 63595616 63596563 63595616 ID=Msa1068570-mRNA-1:exon:13201;Parent=Msa1068570-mRNA-1
chr7_2 exon 63596751 63597160 63596751 ID=Msa1068570-mRNA-1:exon:13200;Parent=Msa1068570-mRNA-1
chr7_2 exon 63597408 63597639 63597408 ID=Msa1068570-mRNA-1:exon:13199;Parent=Msa1068570-mRNA-1
chr7_2 five_prime_UTR 63597408 63597639 63597408 ID=Msa1068570-mRNA-1:five_prime_utr;Parent=Msa1068570-mRNA-1
chr7_2 five_prime_UTR 63597129 63597160 63597129 ID=Msa1068570-mRNA-1:five_prime_utr;Parent=Msa1068570-mRNA-1
chr7_2 CDS 63596751 63597128 63596751 ID=Msa1068570-mRNA-1:cds;Parent=Msa1068570-mRNA-1
chr7_2 CDS 63595970 63596563 63595970 ID=Msa1068570-mRNA-1:cds;Parent=Msa1068570-mRNA-1
chr7_2 three_prime_UTR 63595616 63595969 63595616 ID=Msa1068570-mRNA-1:three_prime_utr;Parent=Msa1068570-mRNA-1
Gene Sequence

>Msa1068570

ATGTGTACACATTTCTTTTTCATGGATAACAGGGGGAAATTTGATAAGATGGTTAAAATATTTACGATTTATGAGTTGGAAAAATTAACTGAAGACTTCAAGTACATGCTGCAATTTGGTGGTTCTGAGAACCAAGAAATCCTCTTGTTGGATGAACTTGAAAATTCAGTGTGCAAATCTGAAAATCTTCGGCGAGTTGCTGTTGATGTTTTGAAGAAACAAATTTTCACGTGGGATGAACTACGACAAAATCAAGAGTTGATCAGAAGGAAATTGATTGATGATGAAATTTTTGATAGAGATGATTATATGTTTAAGATTTCACGCTTAGGAAATCTTAAACCCATTCATTATGGTTTGAAGCCTTCCAATATCCAGAATGTTCCCTTACATACTTTTATAGAGTATCCAACAAATCATAGTAAGTTCCTTATTTGTGTTGATAAAGAGGAGCAAATAAAGAGCACTGCATGGAATTTACACAAAAATGAACCGTGTGTTTTTTACATTGAATTTACACAACCATTAGCCAAGGTTGGAAAAATGACTTCACAATTGCAACACAACTCCACTAGTAGTAATAATCCAAATAAGCACAAGGAAACCGTGCCAATATCATATCCTAAATTTCAGCACAAACGTAAGGAAATTGCACAATATGTTTCATTTGTTGTAATCTTGACCATTATATTTGGTTCCCTTTTCTTCGACACGTGGATCGAGCCATCCCTTATGAAGGTCCAACGTGGTCAAATATGGGTCTCCTCGCTCAGGCTTTTTGGTTTTATGGCGTTTGTTTGGGCCGTTGCTATAATGATTCTTGTTGGTGTACCATTCGTCAGTGTGCAGAGTGTTGTGTTTATCTTCTGGGCTATTTTGATGCATCTTAAATCTCACAACTCAATCACTGCAATGCGTGTTCAAATACTCGGAATAGTTGTCTTTGGGGTACTTGCTCGTGGAAAATTTTAG
Protein sequence

>Msa1068570

MCTHFFFMDNRGKFDKMVKIFTIYELEKLTEDFKYMLQFGGSENQEILLLDELENSVCKSENLRRVAVDVLKKQIFTWDELRQNQELIRRKLIDDEIFDRDDYMFKISRLGNLKPIHYGLKPSNIQNVPLHTFIEYPTNHSKFLICVDKEEQIKSTAWNLHKNEPCVFYIEFTQPLAKVGKMTSQLQHNSTSSNNPNKHKETVPISYPKFQHKRKEIAQYVSFVVILTIIFGSLFFDTWIEPSLMKVQRGQIWVSSLRLFGFMAFVWAVAIMILVGVPFVSVQSVVFIFWAILMHLKSHNSITAMRVQILGIVVFGVLARGKF*