AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1158350


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1158350 MtrunA17_Chr7g0252891 87.500 136 7 1 1 126 12 147 1.36e-79 243
Msa1158350 MtrunA17_Chr4g0015031 87.500 136 7 1 1 126 1 136 7.74e-78 238
Msa1158350 MtrunA17_Chr7g0252921 73.984 123 21 2 14 126 4 125 2.21e-56 182
Msa1158350 MtrunA17_Chr7g0252911 70.536 112 22 2 25 126 16 126 2.32e-49 164
Msa1158350 MtrunA17_Chr7g0252881 71.429 112 21 2 25 126 16 126 3.27e-47 158
Msa1158350 MtrunA17_Chr7g0252861 55.000 120 43 2 17 126 31 149 3.00e-37 132
Msa1158350 MtrunA17_Chr6g0461671 51.754 114 44 2 23 126 50 162 5.45e-30 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1158350 AT5G18280.1 48.214 112 48 1 25 126 41 152 5.42e-26 102
Msa1158350 AT3G04080.1 55.422 83 27 1 54 126 69 151 1.40e-24 98.2
Msa1158350 AT1G14250.1 37.037 81 39 1 55 123 71 151 1.82e-11 61.2
Msa1158350 AT1G14230.1 33.981 103 52 2 33 123 63 161 2.08e-11 60.8

Find 38 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGAAATTGTTTCTTGTTT+TGG 0.150484 7_4:-47641829 None:intergenic
ACATGTTGTTTCCAAGAAAT+TGG 0.163680 7_4:-47641783 None:intergenic
ATTTCTTACTCCCAATTTCT+TGG 0.220318 7_4:+47641772 Msa1158350:CDS
AAAATTAGTTAGGAACATTT+AGG 0.247359 7_4:-47642714 None:intergenic
CATAAAGTTCATCATATTCT+TGG 0.284290 7_4:-47641713 None:intergenic
TTTCTCCATATGTAAGAGTT+AGG 0.302396 7_4:+47642513 Msa1158350:three_prime_UTR
CCAAGTCTAACGGGTGTCTT+GGG 0.304787 7_4:-47642371 None:intergenic
GTCTTGGGGCGTTGGTCCTC+AGG 0.350518 7_4:-47642356 None:intergenic
ATCGTTGATCAATCTATTGT+TGG 0.350731 7_4:+47642582 Msa1158350:three_prime_UTR
ATTATAAACGAAAATTAGTT+AGG 0.357213 7_4:-47642724 None:intergenic
CCCAAGTCTAACGGGTGTCT+TGG 0.371819 7_4:-47642372 None:intergenic
AATAGATTGATCAACGATAA+AGG 0.374549 7_4:-47642577 None:intergenic
CATGTTGTTTCCAAGAAATT+GGG 0.378180 7_4:-47641782 None:intergenic
AAGAAATTGGGAGTAAGAAA+TGG 0.403150 7_4:-47641770 None:intergenic
ATCAAACACAACAGCATAAG+AGG 0.406879 7_4:-47641848 None:intergenic
CCCAAGACACCCGTTAGACT+TGG 0.424438 7_4:+47642371 Msa1158350:CDS
TATGCTGTTGTGTTTGATGC+TGG 0.431064 7_4:+47641853 Msa1158350:CDS
ATCCGCGTATGCACTCAAAC+CGG 0.434433 7_4:-47642273 None:intergenic
CAATTCCTAACTCTTACATA+TGG 0.439155 7_4:-47642518 None:intergenic
AAACTTACCCCAAGTCTAAC+GGG 0.466778 7_4:-47642380 None:intergenic
TAACGGGTGTCTTGGGGCGT+TGG 0.467981 7_4:-47642364 None:intergenic
GTGTTTGATGCTGGTAGCAC+TGG 0.488893 7_4:+47641862 Msa1158350:CDS
CTTTCTGCCTGCTCTAAAAG+TGG 0.495493 7_4:-47642326 None:intergenic
ATTAGTCGAGGTTAAATGAT+GGG 0.503006 7_4:-47642448 None:intergenic
CCAAGACACCCGTTAGACTT+GGG 0.518452 7_4:+47642372 Msa1158350:CDS
TCCGCGTATGCACTCAAACC+GGG 0.534325 7_4:-47642272 None:intergenic
TATTAGTCGAGGTTAAATGA+TGG 0.537559 7_4:-47642449 None:intergenic
AAAACTTACCCCAAGTCTAA+CGG 0.558815 7_4:-47642381 None:intergenic
TTTGATCAAAATGGTAAACA+TGG 0.580756 7_4:-47641894 None:intergenic
TTTAGAGCAGGCAGAAAGTG+TGG 0.588867 7_4:+47642331 Msa1158350:CDS
GGCAGAAAGTGTGGTTCCTG+AGG 0.596755 7_4:+47642340 Msa1158350:CDS
AATTGGGAGTAAGAAATGGA+AGG 0.610820 7_4:-47641766 None:intergenic
CAAGACACCCGTTAGACTTG+GGG 0.616733 7_4:+47642373 Msa1158350:CDS
CAAAATGGTAAACATGGACA+CGG 0.617625 7_4:-47641888 None:intergenic
TTAGTCGAGGTTAAATGATG+GGG 0.618302 7_4:-47642447 None:intergenic
CAAGTCTAACGGGTGTCTTG+GGG 0.619279 7_4:-47642370 None:intergenic
ACCCGGTTTGAGTGCATACG+CGG 0.634041 7_4:+47642271 Msa1158350:CDS
CCTTGTAAAAGATTGAACCG+TGG 0.709941 7_4:+47642627 Msa1158350:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGCATTTAAAAAAAATAA+TGG + chr7_4:47642027-47642046 Msa1158350:intron 10.0%
!! TATGCATTTAAAAAAAATAA+TGG + chr7_4:47642027-47642046 Msa1158350:intron 10.0%
!!! ATTTAGGATATAAAGAAAAA+CGG - chr7_4:47642701-47642720 None:intergenic 15.0%
!! ATTATAAACGAAAATTAGTT+AGG - chr7_4:47642727-47642746 None:intergenic 15.0%
!!! ATTTAGGATATAAAGAAAAA+CGG - chr7_4:47642701-47642720 None:intergenic 15.0%
!! ATTATAAACGAAAATTAGTT+AGG - chr7_4:47642727-47642746 None:intergenic 15.0%
!!! GATTTAAGTTTTGATCAAAA+TGG - chr7_4:47641906-47641925 None:intergenic 20.0%
!!! TGATGTTGAGTTTTATAATA+AGG + chr7_4:47641942-47641961 Msa1158350:intron 20.0%
!! AGTAAAACATAGTCTTTAAT+AGG + chr7_4:47642187-47642206 Msa1158350:intron 20.0%
!! AAAATTAGTTAGGAACATTT+AGG - chr7_4:47642717-47642736 None:intergenic 20.0%
!!! GATTTAAGTTTTGATCAAAA+TGG - chr7_4:47641906-47641925 None:intergenic 20.0%
!!! TGATGTTGAGTTTTATAATA+AGG + chr7_4:47641942-47641961 Msa1158350:intron 20.0%
!! AGTAAAACATAGTCTTTAAT+AGG + chr7_4:47642187-47642206 Msa1158350:intron 20.0%
!! AAAATTAGTTAGGAACATTT+AGG - chr7_4:47642717-47642736 None:intergenic 20.0%
! CATAAAGTTCATCATATTCT+TGG - chr7_4:47641716-47641735 None:intergenic 25.0%
!!! AGGAAATTGTTTCTTGTTTT+GGG - chr7_4:47641831-47641850 None:intergenic 25.0%
! TTTGATCAAAATGGTAAACA+TGG - chr7_4:47641897-47641916 None:intergenic 25.0%
! AACTTAAATCTTCTTCATGT+TGG + chr7_4:47641916-47641935 Msa1158350:intron 25.0%
! AAAGAATATGGTGAAGTTAA+AGG - chr7_4:47642241-47642260 None:intergenic 25.0%
! GAGAAAAATTCACTTTATGT+GGG - chr7_4:47642481-47642500 None:intergenic 25.0%
! AGAGAAAAATTCACTTTATG+TGG - chr7_4:47642482-47642501 None:intergenic 25.0%
! AATAGATTGATCAACGATAA+AGG - chr7_4:47642580-47642599 None:intergenic 25.0%
! CATAAAGTTCATCATATTCT+TGG - chr7_4:47641716-47641735 None:intergenic 25.0%
!!! AGGAAATTGTTTCTTGTTTT+GGG - chr7_4:47641831-47641850 None:intergenic 25.0%
! TTTGATCAAAATGGTAAACA+TGG - chr7_4:47641897-47641916 None:intergenic 25.0%
! AACTTAAATCTTCTTCATGT+TGG + chr7_4:47641916-47641935 Msa1158350:intron 25.0%
! AAAGAATATGGTGAAGTTAA+AGG - chr7_4:47642241-47642260 None:intergenic 25.0%
! GAGAAAAATTCACTTTATGT+GGG - chr7_4:47642481-47642500 None:intergenic 25.0%
! AGAGAAAAATTCACTTTATG+TGG - chr7_4:47642482-47642501 None:intergenic 25.0%
! AATAGATTGATCAACGATAA+AGG - chr7_4:47642580-47642599 None:intergenic 25.0%
AAGAAATTGGGAGTAAGAAA+TGG - chr7_4:47641773-47641792 None:intergenic 30.0%
ATTTCTTACTCCCAATTTCT+TGG + chr7_4:47641772-47641791 Msa1158350:CDS 30.0%
!! CATGTTGTTTCCAAGAAATT+GGG - chr7_4:47641785-47641804 None:intergenic 30.0%
!! ACATGTTGTTTCCAAGAAAT+TGG - chr7_4:47641786-47641805 None:intergenic 30.0%
!!! CTTGTTTTGGGAATATCTTA+CGG - chr7_4:47641819-47641838 None:intergenic 30.0%
!! GAGGAAATTGTTTCTTGTTT+TGG - chr7_4:47641832-47641851 None:intergenic 30.0%
! AGGTGACAATAACATCTTTT+TGG + chr7_4:47641962-47641981 Msa1158350:intron 30.0%
CAAACAATTCTTCACTTCAA+TGG + chr7_4:47642090-47642109 Msa1158350:intron 30.0%
GTGTAGTCTACAAAAGAATA+TGG - chr7_4:47642253-47642272 None:intergenic 30.0%
ATTAGTCGAGGTTAAATGAT+GGG - chr7_4:47642451-47642470 None:intergenic 30.0%
TATTAGTCGAGGTTAAATGA+TGG - chr7_4:47642452-47642471 None:intergenic 30.0%
TTTCTCCATATGTAAGAGTT+AGG + chr7_4:47642513-47642532 Msa1158350:three_prime_UTR 30.0%
CAATTCCTAACTCTTACATA+TGG - chr7_4:47642521-47642540 None:intergenic 30.0%
ATCGTTGATCAATCTATTGT+TGG + chr7_4:47642582-47642601 Msa1158350:three_prime_UTR 30.0%
AAGAAATTGGGAGTAAGAAA+TGG - chr7_4:47641773-47641792 None:intergenic 30.0%
ATTTCTTACTCCCAATTTCT+TGG + chr7_4:47641772-47641791 Msa1158350:CDS 30.0%
!! CATGTTGTTTCCAAGAAATT+GGG - chr7_4:47641785-47641804 None:intergenic 30.0%
!! ACATGTTGTTTCCAAGAAAT+TGG - chr7_4:47641786-47641805 None:intergenic 30.0%
!!! CTTGTTTTGGGAATATCTTA+CGG - chr7_4:47641819-47641838 None:intergenic 30.0%
!! GAGGAAATTGTTTCTTGTTT+TGG - chr7_4:47641832-47641851 None:intergenic 30.0%
! AGGTGACAATAACATCTTTT+TGG + chr7_4:47641962-47641981 Msa1158350:intron 30.0%
CAAACAATTCTTCACTTCAA+TGG + chr7_4:47642090-47642109 Msa1158350:intron 30.0%
GTGTAGTCTACAAAAGAATA+TGG - chr7_4:47642253-47642272 None:intergenic 30.0%
ATTAGTCGAGGTTAAATGAT+GGG - chr7_4:47642451-47642470 None:intergenic 30.0%
TATTAGTCGAGGTTAAATGA+TGG - chr7_4:47642452-47642471 None:intergenic 30.0%
TTTCTCCATATGTAAGAGTT+AGG + chr7_4:47642513-47642532 Msa1158350:three_prime_UTR 30.0%
CAATTCCTAACTCTTACATA+TGG - chr7_4:47642521-47642540 None:intergenic 30.0%
ATCGTTGATCAATCTATTGT+TGG + chr7_4:47642582-47642601 Msa1158350:three_prime_UTR 30.0%
AATTGGGAGTAAGAAATGGA+AGG - chr7_4:47641769-47641788 None:intergenic 35.0%
ATCAAACACAACAGCATAAG+AGG - chr7_4:47641851-47641870 None:intergenic 35.0%
CAAAATGGTAAACATGGACA+CGG - chr7_4:47641891-47641910 None:intergenic 35.0%
! ATTCTTTTGTAGACTACACC+CGG + chr7_4:47642254-47642273 Msa1158350:intron 35.0%
! AAAGATTTTGCAGCTTCTTC+TGG - chr7_4:47642302-47642321 None:intergenic 35.0%
!!! TTTGATTCCACTTTTAGAGC+AGG + chr7_4:47642319-47642338 Msa1158350:CDS 35.0%
AAAACTTACCCCAAGTCTAA+CGG - chr7_4:47642384-47642403 None:intergenic 35.0%
TTAGTCGAGGTTAAATGATG+GGG - chr7_4:47642450-47642469 None:intergenic 35.0%
GTGGGTAAAAATATTAGTCG+AGG - chr7_4:47642463-47642482 None:intergenic 35.0%
!!! GTCTCTACTTTTTTTGTCCA+CGG - chr7_4:47642647-47642666 None:intergenic 35.0%
AATTGGGAGTAAGAAATGGA+AGG - chr7_4:47641769-47641788 None:intergenic 35.0%
ATCAAACACAACAGCATAAG+AGG - chr7_4:47641851-47641870 None:intergenic 35.0%
CAAAATGGTAAACATGGACA+CGG - chr7_4:47641891-47641910 None:intergenic 35.0%
! ATTCTTTTGTAGACTACACC+CGG + chr7_4:47642254-47642273 Msa1158350:intron 35.0%
! AAAGATTTTGCAGCTTCTTC+TGG - chr7_4:47642302-47642321 None:intergenic 35.0%
!!! TTTGATTCCACTTTTAGAGC+AGG + chr7_4:47642319-47642338 Msa1158350:CDS 35.0%
AAAACTTACCCCAAGTCTAA+CGG - chr7_4:47642384-47642403 None:intergenic 35.0%
TTAGTCGAGGTTAAATGATG+GGG - chr7_4:47642450-47642469 None:intergenic 35.0%
GTGGGTAAAAATATTAGTCG+AGG - chr7_4:47642463-47642482 None:intergenic 35.0%
!!! GTCTCTACTTTTTTTGTCCA+CGG - chr7_4:47642647-47642666 None:intergenic 35.0%
TATGCTGTTGTGTTTGATGC+TGG + chr7_4:47641853-47641872 Msa1158350:CDS 40.0%
AAACTTACCCCAAGTCTAAC+GGG - chr7_4:47642383-47642402 None:intergenic 40.0%
! CCACGGTTCAATCTTTTACA+AGG - chr7_4:47642630-47642649 None:intergenic 40.0%
CCTTGTAAAAGATTGAACCG+TGG + chr7_4:47642627-47642646 Msa1158350:three_prime_UTR 40.0%
TATGCTGTTGTGTTTGATGC+TGG + chr7_4:47641853-47641872 Msa1158350:CDS 40.0%
AAACTTACCCCAAGTCTAAC+GGG - chr7_4:47642383-47642402 None:intergenic 40.0%
! CCACGGTTCAATCTTTTACA+AGG - chr7_4:47642630-47642649 None:intergenic 40.0%
CCTTGTAAAAGATTGAACCG+TGG + chr7_4:47642627-47642646 Msa1158350:three_prime_UTR 40.0%
!! CTTTCTGCCTGCTCTAAAAG+TGG - chr7_4:47642329-47642348 None:intergenic 45.0%
! TTTAGAGCAGGCAGAAAGTG+TGG + chr7_4:47642331-47642350 Msa1158350:CDS 45.0%
!! CTTTCTGCCTGCTCTAAAAG+TGG - chr7_4:47642329-47642348 None:intergenic 45.0%
! TTTAGAGCAGGCAGAAAGTG+TGG + chr7_4:47642331-47642350 Msa1158350:CDS 45.0%
GTGTTTGATGCTGGTAGCAC+TGG + chr7_4:47641862-47641881 Msa1158350:CDS 50.0%
ATCCGCGTATGCACTCAAAC+CGG - chr7_4:47642276-47642295 None:intergenic 50.0%
! CAAGTCTAACGGGTGTCTTG+GGG - chr7_4:47642373-47642392 None:intergenic 50.0%
! CCAAGTCTAACGGGTGTCTT+GGG - chr7_4:47642374-47642393 None:intergenic 50.0%
CCAAGACACCCGTTAGACTT+GGG + chr7_4:47642372-47642391 Msa1158350:CDS 50.0%
CAAGACACCCGTTAGACTTG+GGG + chr7_4:47642373-47642392 Msa1158350:CDS 50.0%
GTGTTTGATGCTGGTAGCAC+TGG + chr7_4:47641862-47641881 Msa1158350:CDS 50.0%
ATCCGCGTATGCACTCAAAC+CGG - chr7_4:47642276-47642295 None:intergenic 50.0%
! CAAGTCTAACGGGTGTCTTG+GGG - chr7_4:47642373-47642392 None:intergenic 50.0%
! CCAAGTCTAACGGGTGTCTT+GGG - chr7_4:47642374-47642393 None:intergenic 50.0%
CCAAGACACCCGTTAGACTT+GGG + chr7_4:47642372-47642391 Msa1158350:CDS 50.0%
CAAGACACCCGTTAGACTTG+GGG + chr7_4:47642373-47642392 Msa1158350:CDS 50.0%
! ACCCGGTTTGAGTGCATACG+CGG + chr7_4:47642271-47642290 Msa1158350:CDS 55.0%
TCCGCGTATGCACTCAAACC+GGG - chr7_4:47642275-47642294 None:intergenic 55.0%
! GGCAGAAAGTGTGGTTCCTG+AGG + chr7_4:47642340-47642359 Msa1158350:CDS 55.0%
CCCAAGACACCCGTTAGACT+TGG + chr7_4:47642371-47642390 Msa1158350:CDS 55.0%
! CCCAAGTCTAACGGGTGTCT+TGG - chr7_4:47642375-47642394 None:intergenic 55.0%
! ACCCGGTTTGAGTGCATACG+CGG + chr7_4:47642271-47642290 Msa1158350:CDS 55.0%
TCCGCGTATGCACTCAAACC+GGG - chr7_4:47642275-47642294 None:intergenic 55.0%
! GGCAGAAAGTGTGGTTCCTG+AGG + chr7_4:47642340-47642359 Msa1158350:CDS 55.0%
CCCAAGACACCCGTTAGACT+TGG + chr7_4:47642371-47642390 Msa1158350:CDS 55.0%
! CCCAAGTCTAACGGGTGTCT+TGG - chr7_4:47642375-47642394 None:intergenic 55.0%
! TAACGGGTGTCTTGGGGCGT+TGG - chr7_4:47642367-47642386 None:intergenic 60.0%
! TAACGGGTGTCTTGGGGCGT+TGG - chr7_4:47642367-47642386 None:intergenic 60.0%
!! GTCTTGGGGCGTTGGTCCTC+AGG - chr7_4:47642359-47642378 None:intergenic 65.0%
!! GTCTTGGGGCGTTGGTCCTC+AGG - chr7_4:47642359-47642378 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 47641691 47642772 47641691 ID=Msa1158350;Name=Msa1158350
chr7_4 mRNA 47641691 47642772 47641691 ID=Msa1158350-mRNA-1;Parent=Msa1158350;Name=Msa1158350-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0.5|1|0|0.5|2|303|148
chr7_4 exon 47641691 47641933 47641691 ID=Msa1158350-mRNA-1:exon:12735;Parent=Msa1158350-mRNA-1
chr7_4 exon 47642266 47642772 47642266 ID=Msa1158350-mRNA-1:exon:12736;Parent=Msa1158350-mRNA-1
chr7_4 CDS 47641691 47641933 47641691 ID=Msa1158350-mRNA-1:cds;Parent=Msa1158350-mRNA-1
chr7_4 CDS 47642266 47642469 47642266 ID=Msa1158350-mRNA-1:cds;Parent=Msa1158350-mRNA-1
chr7_4 three_prime_UTR 47642470 47642772 47642470 ID=Msa1158350-mRNA-1:three_prime_utr;Parent=Msa1158350-mRNA-1
Gene Sequence

>Msa1158350

ATGGTTTTGCTTTGGCAAAACACCAAGAATATGATGAACTTTATGACACTCATCACATTTCTACTCTTCATTATGCCTTCCATTTCTTACTCCCAATTTCTTGGAAACAACATGTTACTCACTAACCGTAAGATATTCCCAAAACAAGAAACAATTTCCTCTTATGCTGTTGTGTTTGATGCTGGTAGCACTGGAAGCCGTGTCCATGTTTACCATTTTGATCAAAACTTAAATCTTCTTCATACTACACCCGGTTTGAGTGCATACGCGGATAATCCAGAAGAAGCTGCAAAATCTTTGATTCCACTTTTAGAGCAGGCAGAAAGTGTGGTTCCTGAGGACCAACGCCCCAAGACACCCGTTAGACTTGGGGTAAGTTTTTTCGTGTATTTTTTTATATATCATGTTATAGATATTGAGTTTTCCCCATCATTTAACCTCGACTAA

Protein sequence

>Msa1158350

MVLLWQNTKNMMNFMTLITFLLFIMPSISYSQFLGNNMLLTNRKIFPKQETISSYAVVFDAGSTGSRVHVYHFDQNLNLLHTTPGLSAYADNPEEAAKSLIPLLEQAESVVPEDQRPKTPVRLGVSFFVYFFIYHVIDIEFSPSFNLD*