AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1338520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1338520 MtrunA17_Chr7g0252891 97.719 263 6 0 1 263 216 478 0.0 539
Msa1338520 MtrunA17_Chr4g0015031 87.452 263 32 1 1 263 205 466 1.29e-161 456
Msa1338520 MtrunA17_Chr7g0252881 81.749 263 47 1 1 263 195 456 5.71e-154 436
Msa1338520 MtrunA17_Chr7g0252911 68.061 263 82 2 1 263 195 455 3.13e-128 371
Msa1338520 MtrunA17_Chr7g0252921 66.667 261 85 2 2 262 195 453 7.06e-116 340
Msa1338520 MtrunA17_Chr7g0252861 59.684 253 97 4 1 252 218 466 4.50e-93 282
Msa1338520 MtrunA17_Chr6g0461671 59.449 254 100 3 1 253 231 482 4.53e-91 277
Msa1338520 MtrunA17_Chr7g0252941 58.523 176 71 2 49 224 6 179 2.90e-59 186
Msa1338520 MtrunA17_Chr7g0252901 62.037 108 31 3 156 263 3 100 6.63e-37 126
Msa1338520 MtrunA17_Chr7g0252931 61.538 52 18 1 2 53 21 70 9.33e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1338520 AT5G18280.1 58.103 253 103 3 1 252 221 471 1.34e-93 284
Msa1338520 AT3G04080.1 55.336 253 110 3 1 252 220 470 1.78e-86 265

Find 69 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACTATGCATTGGGGAAAT+TGG 0.164398 tig0002256:+684 Msa1338520:five_prime_UTR
GTGAGACTATAAGGTGTATT+TGG 0.179514 tig0002256:-1661 None:intergenic
TACATCAATCGTTCAAATTT+AGG 0.187026 tig0002256:-2047 None:intergenic
TCTTCTCCATCTGCAACTTT+TGG 0.192928 tig0002256:-802 None:intergenic
CTTACCTAAGGTAATTAAAT+TGG 0.195132 tig0002256:-124 None:intergenic
AAGGCCAATGCCCCTGCTTC+TGG 0.233678 tig0002256:+1341 Msa1338520:CDS
CCCAGAGATAAGAACCTTCT+TGG 0.271914 tig0002256:-213 None:intergenic
CCTTATCAGAATTGCACTTT+TGG 0.272682 tig0002256:+1425 Msa1338520:CDS
AACTATGCATTGGGGAAATT+GGG 0.305765 tig0002256:+685 Msa1338520:five_prime_UTR
ATATGCTGGAGAAGAATTTA+AGG 0.312341 tig0002256:+1322 Msa1338520:CDS
AAATTTAGGTAACGATGATA+TGG 0.324810 tig0002256:-2033 None:intergenic
AATATGATCTCTATGTTCAT+AGG 0.325141 tig0002256:+860 Msa1338520:CDS
AGTACAAGCTTCTTAATGTA+TGG 0.360656 tig0002256:-826 None:intergenic
CAGTACGTGTTGCTCGTTGA+TGG 0.375641 tig0002256:+1814 Msa1338520:CDS
GGTGGAATTTGGAGTGGTGG+AGG 0.381650 tig0002256:+1446 Msa1338520:CDS
AAGAAGTGGATAAGTTGATT+TGG 0.384688 tig0002256:-1746 None:intergenic
GGACGAAGTGTGAGACTATA+AGG 0.386803 tig0002256:-1670 None:intergenic
TTAAAACTGGCTCCAGAAGC+AGG 0.407764 tig0002256:-1353 None:intergenic
GTGACAGTTAACTATGCATT+GGG 0.413726 tig0002256:+676 Msa1338520:five_prime_UTR
GGCTCCAGAAGCAGGGGCAT+TGG 0.420983 tig0002256:-1345 None:intergenic
TTGAGAATATTGATAGCTTA+TGG 0.431015 tig0002256:+2203 Msa1338520:three_prime_UTR
TGAGACTATAAGGTGTATTT+GGG 0.440417 tig0002256:-1660 None:intergenic
TGGAGTGGTGGAGGAGGAAG+TGG 0.447986 tig0002256:+1455 Msa1338520:CDS
GTGTTGCTCGTTGATGGATT+TGG 0.451160 tig0002256:+1820 Msa1338520:CDS
CCAGAGATAAGAACCTTCTT+GGG 0.465441 tig0002256:-212 None:intergenic
ATCAACTTATCCACTTCTTG+TGG 0.471679 tig0002256:+1750 Msa1338520:CDS
TGCATCACGTTGAACGTTGA+AGG 0.472392 tig0002256:-170 None:intergenic
CCAGCTAAAATGCAAGGGTT+AGG 0.472739 tig0002256:-1029 None:intergenic
TTTGGTGGAATTTGGAGTGG+TGG 0.483888 tig0002256:+1443 Msa1338520:CDS
GTGGAAGCTGCATGGCCTCT+AGG 0.485627 tig0002256:+1996 Msa1338520:CDS
TGGAGGTGGATCGGTTCAAA+TGG 0.485873 tig0002256:+744 Msa1338520:CDS
CCAAAAGTGCAATTCTGATA+AGG 0.489796 tig0002256:-1425 None:intergenic
ACAGTGGGAGTAATGGATCT+TGG 0.493110 tig0002256:+724 Msa1338520:exon
CTTTGGTAGAGAAGCATCTC+GGG 0.495672 tig0002256:+977 Msa1338520:CDS
GGAATTTGGAGTGGTGGAGG+AGG 0.496019 tig0002256:+1449 Msa1338520:CDS
AAAGGGACATACACATATGC+TGG 0.509416 tig0002256:+1308 Msa1338520:intron
CCCGCTGTAATCTCTTGCAA+TGG 0.520889 tig0002256:-1945 None:intergenic
TTTAGTAGAATATGTTTGCA+TGG 0.522109 tig0002256:+1780 Msa1338520:CDS
CATAAACAAAACAAGCTCAA+TGG 0.522666 tig0002256:-2170 None:intergenic
GGTGACAGTTAACTATGCAT+TGG 0.523428 tig0002256:+675 Msa1338520:intron
CATTAAGAAGCTTGTACTCA+AGG 0.525184 tig0002256:+831 Msa1338520:CDS
TCTTCCAGTTACTTACACTT+TGG 0.525363 tig0002256:+960 Msa1338520:intron
TAAAACTGGCTCCAGAAGCA+GGG 0.545057 tig0002256:-1352 None:intergenic
CTGAACAAATCCCTTACCTA+AGG 0.547857 tig0002256:-136 None:intergenic
AAATTGAAATCCACAAGAAG+TGG 0.551668 tig0002256:-1760 None:intergenic
ACTTTGGTAGAGAAGCATCT+CGG 0.551865 tig0002256:+976 Msa1338520:CDS
ACTATGCATTGGGGAAATTG+GGG 0.553135 tig0002256:+686 Msa1338520:five_prime_UTR
TCAAATCCAGCTAAAATGCA+AGG 0.555070 tig0002256:-1035 None:intergenic
GGAGTAATGGATCTTGGAGG+TGG 0.558456 tig0002256:+730 Msa1338520:exon
TGAATATCAAGATTCTCTTG+TGG 0.566345 tig0002256:+1977 Msa1338520:CDS
GCTTCAGTCTCAAGATCCAC+AGG 0.567472 tig0002256:-1691 None:intergenic
AGCTTGTACATTAAATCTTG+AGG 0.568041 tig0002256:+1720 Msa1338520:CDS
AATGCTCCAAAAGTTGCAGA+TGG 0.572535 tig0002256:+796 Msa1338520:CDS
TAGTCTCACACTTCGTCCTG+TGG 0.572847 tig0002256:+1675 Msa1338520:CDS
AATGGATCTTGGAGGTGGAT+CGG 0.580364 tig0002256:+735 Msa1338520:exon
ATTAAGAAGCTTGTACTCAA+GGG 0.588255 tig0002256:+832 Msa1338520:CDS
AATTAAGCTTAATTAAAGCA+TGG 0.589338 tig0002256:-94 None:intergenic
ATTCTCTTGTGGAAGCTGCA+TGG 0.602647 tig0002256:+1988 Msa1338520:CDS
TAATGCATACATCTTCAGCT+AGG 0.603926 tig0002256:-1514 None:intergenic
GTGGGAGTAATGGATCTTGG+AGG 0.612375 tig0002256:+727 Msa1338520:exon
CCATTGCAAGAGATTACAGC+GGG 0.615493 tig0002256:+1945 Msa1338520:CDS
CAAATCCAGCTAAAATGCAA+GGG 0.619736 tig0002256:-1034 None:intergenic
AAAACTGGCTCCAGAAGCAG+GGG 0.621311 tig0002256:-1351 None:intergenic
AAATGGCATATGCAGTGTCA+AGG 0.621886 tig0002256:+761 Msa1338520:CDS
GTGCAATTCTGATAAGGACA+TGG 0.628525 tig0002256:-1419 None:intergenic
TTGTACATTAAATCTTGAGG+AGG 0.647392 tig0002256:+1723 Msa1338520:CDS
GCTTCTACAGCATTTCCTAG+AGG 0.686619 tig0002256:-2011 None:intergenic
TCCATTGCAAGAGATTACAG+CGG 0.727640 tig0002256:+1944 Msa1338520:CDS
TGACAGTTAACTATGCATTG+GGG 0.729371 tig0002256:+677 Msa1338520:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTTTATTTTTAAAACT+CGG - tig0002256:1215-1234 None:intergenic 10.0%
!! TAAAAATAGAGAAATTAAAA+AGG - tig0002256:1261-1280 None:intergenic 10.0%
!! ATCATTAAATCAAAATAATT+TGG - tig0002256:1577-1596 None:intergenic 10.0%
!!! AGTTTTTTATTTTTAAAACT+CGG - tig0002256:1215-1234 None:intergenic 10.0%
!! TAAAAATAGAGAAATTAAAA+AGG - tig0002256:1261-1280 None:intergenic 10.0%
!! ATCATTAAATCAAAATAATT+TGG - tig0002256:1577-1596 None:intergenic 10.0%
!!! AAAATTGCAAATTTTCAAAT+GGG + tig0002256:8-27 Msa1338520:five_prime_UTR 15.0%
!!! TCATATAAGAATTTTATTTG+TGG + tig0002256:283-302 Msa1338520:intron 15.0%
!!! TACAACAAATATTTTTAGAA+AGG + tig0002256:475-494 Msa1338520:intron 15.0%
!!! ATATTTTTAGAAAGGAAAAA+TGG + tig0002256:483-502 Msa1338520:intron 15.0%
!! TCTACTTATTACATATTTAA+CGG - tig0002256:621-640 None:intergenic 15.0%
!!! ACAAATATTTTGTTTTTCAA+AGG + tig0002256:1290-1309 Msa1338520:intron 15.0%
!!! CAAATATTTTGTTTTTCAAA+GGG + tig0002256:1291-1310 Msa1338520:intron 15.0%
!! AATAAACAATGTAATTAGAA+TGG - tig0002256:1619-1638 None:intergenic 15.0%
!!! CATTGTTTATTATTTTAGAT+TGG + tig0002256:1628-1647 Msa1338520:intron 15.0%
!!! TTTATTATTTTAGATTGGTT+TGG + tig0002256:1633-1652 Msa1338520:intron 15.0%
!!! TAGTTTAAAACAAATTACAA+AGG - tig0002256:1871-1890 None:intergenic 15.0%
!!! CAATAATTGATTTTATTTGT+TGG + tig0002256:1911-1930 Msa1338520:intron 15.0%
!!! TAAAATATTTGAATTTTCGT+AGG + tig0002256:2232-2251 Msa1338520:three_prime_UTR 15.0%
!!! TTTTTCAATGTTTTAAGATA+AGG + tig0002256:2277-2296 Msa1338520:three_prime_UTR 15.0%
!!! AAAATTGCAAATTTTCAAAT+GGG + tig0002256:8-27 Msa1338520:five_prime_UTR 15.0%
!!! TCATATAAGAATTTTATTTG+TGG + tig0002256:283-302 Msa1338520:intron 15.0%
!!! TACAACAAATATTTTTAGAA+AGG + tig0002256:475-494 Msa1338520:intron 15.0%
!!! ATATTTTTAGAAAGGAAAAA+TGG + tig0002256:483-502 Msa1338520:intron 15.0%
!! TCTACTTATTACATATTTAA+CGG - tig0002256:621-640 None:intergenic 15.0%
!!! ACAAATATTTTGTTTTTCAA+AGG + tig0002256:1290-1309 Msa1338520:intron 15.0%
!!! CAAATATTTTGTTTTTCAAA+GGG + tig0002256:1291-1310 Msa1338520:intron 15.0%
!! AATAAACAATGTAATTAGAA+TGG - tig0002256:1619-1638 None:intergenic 15.0%
!!! CATTGTTTATTATTTTAGAT+TGG + tig0002256:1628-1647 Msa1338520:intron 15.0%
!!! TTTATTATTTTAGATTGGTT+TGG + tig0002256:1633-1652 Msa1338520:intron 15.0%
!!! TAGTTTAAAACAAATTACAA+AGG - tig0002256:1871-1890 None:intergenic 15.0%
!!! CAATAATTGATTTTATTTGT+TGG + tig0002256:1911-1930 Msa1338520:intron 15.0%
!!! TAAAATATTTGAATTTTCGT+AGG + tig0002256:2232-2251 Msa1338520:three_prime_UTR 15.0%
!!! TTTTTCAATGTTTTAAGATA+AGG + tig0002256:2277-2296 Msa1338520:three_prime_UTR 15.0%
!!! CAAAATTGCAAATTTTCAAA+TGG + tig0002256:7-26 Msa1338520:five_prime_UTR 20.0%
!! AATTAAGCTTAATTAAAGCA+TGG - tig0002256:97-116 None:intergenic 20.0%
!! AATTTAATTACCTTAGGTAA+GGG + tig0002256:126-145 Msa1338520:five_prime_UTR 20.0%
!! AAAAAATTCACAAAAACAGT+GGG + tig0002256:709-728 Msa1338520:five_prime_UTR 20.0%
!! ATATTATATGAAGAACTAGT+GGG - tig0002256:894-913 None:intergenic 20.0%
!! TATATTATATGAAGAACTAG+TGG - tig0002256:895-914 None:intergenic 20.0%
!! TTAAGAGCTCTTAATATATT+AGG - tig0002256:934-953 None:intergenic 20.0%
!!! CTTTTTGCATTTTTTAAAAC+TGG - tig0002256:1369-1388 None:intergenic 20.0%
!! AACATTGAAAAACAAGTTAA+TGG - tig0002256:2269-2288 None:intergenic 20.0%
!!! CAAAATTGCAAATTTTCAAA+TGG + tig0002256:7-26 Msa1338520:five_prime_UTR 20.0%
!! AATTAAGCTTAATTAAAGCA+TGG - tig0002256:97-116 None:intergenic 20.0%
!! AATTTAATTACCTTAGGTAA+GGG + tig0002256:126-145 Msa1338520:five_prime_UTR 20.0%
!! AAAAAATTCACAAAAACAGT+GGG + tig0002256:709-728 Msa1338520:five_prime_UTR 20.0%
!! ATATTATATGAAGAACTAGT+GGG - tig0002256:894-913 None:intergenic 20.0%
!! TATATTATATGAAGAACTAG+TGG - tig0002256:895-914 None:intergenic 20.0%
!! TTAAGAGCTCTTAATATATT+AGG - tig0002256:934-953 None:intergenic 20.0%
!!! CTTTTTGCATTTTTTAAAAC+TGG - tig0002256:1369-1388 None:intergenic 20.0%
!! AACATTGAAAAACAAGTTAA+TGG - tig0002256:2269-2288 None:intergenic 20.0%
! CTTACCTAAGGTAATTAAAT+TGG - tig0002256:127-146 None:intergenic 25.0%
! CAATTTAATTACCTTAGGTA+AGG + tig0002256:125-144 Msa1338520:five_prime_UTR 25.0%
!! TTTGTTATGTCTGTTTGAAT+AGG + tig0002256:308-327 Msa1338520:intron 25.0%
! AAGAAAGTCAAATCTTGAAT+AGG - tig0002256:350-369 None:intergenic 25.0%
! TTGAAACTTGTCATCTTTAT+AGG + tig0002256:654-673 Msa1338520:intron 25.0%
! GAAAAAATTCACAAAAACAG+TGG + tig0002256:708-727 Msa1338520:five_prime_UTR 25.0%
! AATATGATCTCTATGTTCAT+AGG + tig0002256:860-879 Msa1338520:CDS 25.0%
! TTTAGTAGAATATGTTTGCA+TGG + tig0002256:1780-1799 Msa1338520:CDS 25.0%
!!! AATTGATTTTATTTGTTGGC+AGG + tig0002256:1915-1934 Msa1338520:intron 25.0%
! AAATTTAGGTAACGATGATA+TGG - tig0002256:2036-2055 None:intergenic 25.0%
! TACATCAATCGTTCAAATTT+AGG - tig0002256:2050-2069 None:intergenic 25.0%
!! TTGAGAATATTGATAGCTTA+TGG + tig0002256:2203-2222 Msa1338520:three_prime_UTR 25.0%
! CTTACCTAAGGTAATTAAAT+TGG - tig0002256:127-146 None:intergenic 25.0%
! CAATTTAATTACCTTAGGTA+AGG + tig0002256:125-144 Msa1338520:five_prime_UTR 25.0%
!! TTTGTTATGTCTGTTTGAAT+AGG + tig0002256:308-327 Msa1338520:intron 25.0%
! AAGAAAGTCAAATCTTGAAT+AGG - tig0002256:350-369 None:intergenic 25.0%
! TTGAAACTTGTCATCTTTAT+AGG + tig0002256:654-673 Msa1338520:intron 25.0%
! GAAAAAATTCACAAAAACAG+TGG + tig0002256:708-727 Msa1338520:five_prime_UTR 25.0%
! AATATGATCTCTATGTTCAT+AGG + tig0002256:860-879 Msa1338520:CDS 25.0%
! TTTAGTAGAATATGTTTGCA+TGG + tig0002256:1780-1799 Msa1338520:CDS 25.0%
!!! AATTGATTTTATTTGTTGGC+AGG + tig0002256:1915-1934 Msa1338520:intron 25.0%
! AAATTTAGGTAACGATGATA+TGG - tig0002256:2036-2055 None:intergenic 25.0%
! TACATCAATCGTTCAAATTT+AGG - tig0002256:2050-2069 None:intergenic 25.0%
!! TTGAGAATATTGATAGCTTA+TGG + tig0002256:2203-2222 Msa1338520:three_prime_UTR 25.0%
TAATGGTATCATTAGCTGTT+AGG + tig0002256:63-82 Msa1338520:five_prime_UTR 30.0%
TGCTCCAATTTAATTACCTT+AGG + tig0002256:120-139 Msa1338520:five_prime_UTR 30.0%
GATGCAGTTTCTATTATTGA+TGG + tig0002256:187-206 Msa1338520:five_prime_UTR 30.0%
AGAACATACACACACATATA+TGG + tig0002256:450-469 Msa1338520:intron 30.0%
AGTACAAGCTTCTTAATGTA+TGG - tig0002256:829-848 None:intergenic 30.0%
ATTAAGAAGCTTGTACTCAA+GGG + tig0002256:832-851 Msa1338520:CDS 30.0%
AAGTTGACTAAAACAACTCT+CGG + tig0002256:1165-1184 Msa1338520:intron 30.0%
ATATGCTGGAGAAGAATTTA+AGG + tig0002256:1322-1341 Msa1338520:CDS 30.0%
TGAGACTATAAGGTGTATTT+GGG - tig0002256:1663-1682 None:intergenic 30.0%
AGCTTGTACATTAAATCTTG+AGG + tig0002256:1720-1739 Msa1338520:CDS 30.0%
TTGTACATTAAATCTTGAGG+AGG + tig0002256:1723-1742 Msa1338520:CDS 30.0%
!! AAGAAGTGGATAAGTTGATT+TGG - tig0002256:1749-1768 None:intergenic 30.0%
AAATTGAAATCCACAAGAAG+TGG - tig0002256:1763-1782 None:intergenic 30.0%
TGAATATCAAGATTCTCTTG+TGG + tig0002256:1977-1996 Msa1338520:CDS 30.0%
CATAAACAAAACAAGCTCAA+TGG - tig0002256:2173-2192 None:intergenic 30.0%
TAATGGTATCATTAGCTGTT+AGG + tig0002256:63-82 Msa1338520:five_prime_UTR 30.0%
TGCTCCAATTTAATTACCTT+AGG + tig0002256:120-139 Msa1338520:five_prime_UTR 30.0%
GATGCAGTTTCTATTATTGA+TGG + tig0002256:187-206 Msa1338520:five_prime_UTR 30.0%
AGAACATACACACACATATA+TGG + tig0002256:450-469 Msa1338520:intron 30.0%
AGTACAAGCTTCTTAATGTA+TGG - tig0002256:829-848 None:intergenic 30.0%
ATTAAGAAGCTTGTACTCAA+GGG + tig0002256:832-851 Msa1338520:CDS 30.0%
AAGTTGACTAAAACAACTCT+CGG + tig0002256:1165-1184 Msa1338520:intron 30.0%
ATATGCTGGAGAAGAATTTA+AGG + tig0002256:1322-1341 Msa1338520:CDS 30.0%
TGAGACTATAAGGTGTATTT+GGG - tig0002256:1663-1682 None:intergenic 30.0%
AGCTTGTACATTAAATCTTG+AGG + tig0002256:1720-1739 Msa1338520:CDS 30.0%
TTGTACATTAAATCTTGAGG+AGG + tig0002256:1723-1742 Msa1338520:CDS 30.0%
!! AAGAAGTGGATAAGTTGATT+TGG - tig0002256:1749-1768 None:intergenic 30.0%
AAATTGAAATCCACAAGAAG+TGG - tig0002256:1763-1782 None:intergenic 30.0%
TGAATATCAAGATTCTCTTG+TGG + tig0002256:1977-1996 Msa1338520:CDS 30.0%
CATAAACAAAACAAGCTCAA+TGG - tig0002256:2173-2192 None:intergenic 30.0%
TTCAAATGGGTCATCTATTG+CGG + tig0002256:21-40 Msa1338520:five_prime_UTR 35.0%
TCATTAGCTGTTAGGTCTAT+AGG + tig0002256:71-90 Msa1338520:five_prime_UTR 35.0%
ATTATTGATGGAACCCAAGA+AGG + tig0002256:199-218 Msa1338520:five_prime_UTR 35.0%
GTGACAGTTAACTATGCATT+GGG + tig0002256:676-695 Msa1338520:five_prime_UTR 35.0%
TGACAGTTAACTATGCATTG+GGG + tig0002256:677-696 Msa1338520:five_prime_UTR 35.0%
TAACTATGCATTGGGGAAAT+TGG + tig0002256:684-703 Msa1338520:five_prime_UTR 35.0%
AACTATGCATTGGGGAAATT+GGG + tig0002256:685-704 Msa1338520:five_prime_UTR 35.0%
CATTAAGAAGCTTGTACTCA+AGG + tig0002256:831-850 Msa1338520:CDS 35.0%
TCTTCCAGTTACTTACACTT+TGG + tig0002256:960-979 Msa1338520:intron 35.0%
TCTACCAAAGTGTAAGTAAC+TGG - tig0002256:967-986 None:intergenic 35.0%
CAAATCCAGCTAAAATGCAA+GGG - tig0002256:1037-1056 None:intergenic 35.0%
TCAAATCCAGCTAAAATGCA+AGG - tig0002256:1038-1057 None:intergenic 35.0%
!!! TGCATTTTAGCTGGATTTGA+TGG + tig0002256:1038-1057 Msa1338520:CDS 35.0%
ATGCAAAAAGATAGTTCGTC+AGG + tig0002256:1379-1398 Msa1338520:CDS 35.0%
! CCAAAAGTGCAATTCTGATA+AGG - tig0002256:1428-1447 None:intergenic 35.0%
! CCTTATCAGAATTGCACTTT+TGG + tig0002256:1425-1444 Msa1338520:CDS 35.0%
! TATCAGAATTGCACTTTTGG+TGG + tig0002256:1428-1447 Msa1338520:CDS 35.0%
! ATTGCACTTTTGGTGGAATT+TGG + tig0002256:1435-1454 Msa1338520:CDS 35.0%
TAATGCATACATCTTCAGCT+AGG - tig0002256:1517-1536 None:intergenic 35.0%
GTGAGACTATAAGGTGTATT+TGG - tig0002256:1664-1683 None:intergenic 35.0%
ATCAACTTATCCACTTCTTG+TGG + tig0002256:1750-1769 Msa1338520:CDS 35.0%
TTCAAATGGGTCATCTATTG+CGG + tig0002256:21-40 Msa1338520:five_prime_UTR 35.0%
TCATTAGCTGTTAGGTCTAT+AGG + tig0002256:71-90 Msa1338520:five_prime_UTR 35.0%
ATTATTGATGGAACCCAAGA+AGG + tig0002256:199-218 Msa1338520:five_prime_UTR 35.0%
GTGACAGTTAACTATGCATT+GGG + tig0002256:676-695 Msa1338520:five_prime_UTR 35.0%
TGACAGTTAACTATGCATTG+GGG + tig0002256:677-696 Msa1338520:five_prime_UTR 35.0%
TAACTATGCATTGGGGAAAT+TGG + tig0002256:684-703 Msa1338520:five_prime_UTR 35.0%
AACTATGCATTGGGGAAATT+GGG + tig0002256:685-704 Msa1338520:five_prime_UTR 35.0%
CATTAAGAAGCTTGTACTCA+AGG + tig0002256:831-850 Msa1338520:CDS 35.0%
TCTTCCAGTTACTTACACTT+TGG + tig0002256:960-979 Msa1338520:intron 35.0%
TCTACCAAAGTGTAAGTAAC+TGG - tig0002256:967-986 None:intergenic 35.0%
CAAATCCAGCTAAAATGCAA+GGG - tig0002256:1037-1056 None:intergenic 35.0%
TCAAATCCAGCTAAAATGCA+AGG - tig0002256:1038-1057 None:intergenic 35.0%
!!! TGCATTTTAGCTGGATTTGA+TGG + tig0002256:1038-1057 Msa1338520:CDS 35.0%
ATGCAAAAAGATAGTTCGTC+AGG + tig0002256:1379-1398 Msa1338520:CDS 35.0%
! CCAAAAGTGCAATTCTGATA+AGG - tig0002256:1428-1447 None:intergenic 35.0%
! CCTTATCAGAATTGCACTTT+TGG + tig0002256:1425-1444 Msa1338520:CDS 35.0%
! TATCAGAATTGCACTTTTGG+TGG + tig0002256:1428-1447 Msa1338520:CDS 35.0%
! ATTGCACTTTTGGTGGAATT+TGG + tig0002256:1435-1454 Msa1338520:CDS 35.0%
TAATGCATACATCTTCAGCT+AGG - tig0002256:1517-1536 None:intergenic 35.0%
GTGAGACTATAAGGTGTATT+TGG - tig0002256:1664-1683 None:intergenic 35.0%
ATCAACTTATCCACTTCTTG+TGG + tig0002256:1750-1769 Msa1338520:CDS 35.0%
TCAAATGGGTCATCTATTGC+GGG + tig0002256:22-41 Msa1338520:five_prime_UTR 40.0%
CTGAACAAATCCCTTACCTA+AGG - tig0002256:139-158 None:intergenic 40.0%
CCAGAGATAAGAACCTTCTT+GGG - tig0002256:215-234 None:intergenic 40.0%
CCAAGAAGGTTCTTATCTCT+GGG + tig0002256:213-232 Msa1338520:five_prime_UTR 40.0%
TGTGTATGTGTACGCATGTA+TGG - tig0002256:405-424 None:intergenic 40.0%
GGTGACAGTTAACTATGCAT+TGG + tig0002256:675-694 Msa1338520:intron 40.0%
ACTATGCATTGGGGAAATTG+GGG + tig0002256:686-705 Msa1338520:five_prime_UTR 40.0%
CACAAAAACAGTGGGAGTAA+TGG + tig0002256:717-736 Msa1338520:exon 40.0%
AAATGGCATATGCAGTGTCA+AGG + tig0002256:761-780 Msa1338520:CDS 40.0%
AATGCTCCAAAAGTTGCAGA+TGG + tig0002256:796-815 Msa1338520:CDS 40.0%
! TCTTCTCCATCTGCAACTTT+TGG - tig0002256:805-824 None:intergenic 40.0%
!! ACTTTGGTAGAGAAGCATCT+CGG + tig0002256:976-995 Msa1338520:CDS 40.0%
AAAGGGACATACACATATGC+TGG + tig0002256:1308-1327 Msa1338520:intron 40.0%
GTGCAATTCTGATAAGGACA+TGG - tig0002256:1422-1441 None:intergenic 40.0%
! ACTTTTGGTGGAATTTGGAG+TGG + tig0002256:1440-1459 Msa1338520:CDS 40.0%
TCCATTGCAAGAGATTACAG+CGG + tig0002256:1944-1963 Msa1338520:CDS 40.0%
TCAAATGGGTCATCTATTGC+GGG + tig0002256:22-41 Msa1338520:five_prime_UTR 40.0%
CTGAACAAATCCCTTACCTA+AGG - tig0002256:139-158 None:intergenic 40.0%
CCAGAGATAAGAACCTTCTT+GGG - tig0002256:215-234 None:intergenic 40.0%
CCAAGAAGGTTCTTATCTCT+GGG + tig0002256:213-232 Msa1338520:five_prime_UTR 40.0%
TGTGTATGTGTACGCATGTA+TGG - tig0002256:405-424 None:intergenic 40.0%
GGTGACAGTTAACTATGCAT+TGG + tig0002256:675-694 Msa1338520:intron 40.0%
ACTATGCATTGGGGAAATTG+GGG + tig0002256:686-705 Msa1338520:five_prime_UTR 40.0%
CACAAAAACAGTGGGAGTAA+TGG + tig0002256:717-736 Msa1338520:exon 40.0%
AAATGGCATATGCAGTGTCA+AGG + tig0002256:761-780 Msa1338520:CDS 40.0%
AATGCTCCAAAAGTTGCAGA+TGG + tig0002256:796-815 Msa1338520:CDS 40.0%
! TCTTCTCCATCTGCAACTTT+TGG - tig0002256:805-824 None:intergenic 40.0%
!! ACTTTGGTAGAGAAGCATCT+CGG + tig0002256:976-995 Msa1338520:CDS 40.0%
AAAGGGACATACACATATGC+TGG + tig0002256:1308-1327 Msa1338520:intron 40.0%
GTGCAATTCTGATAAGGACA+TGG - tig0002256:1422-1441 None:intergenic 40.0%
! ACTTTTGGTGGAATTTGGAG+TGG + tig0002256:1440-1459 Msa1338520:CDS 40.0%
TCCATTGCAAGAGATTACAG+CGG + tig0002256:1944-1963 Msa1338520:CDS 40.0%
CGGAGGGAGTAGTTCAATAA+TGG + tig0002256:46-65 Msa1338520:five_prime_UTR 45.0%
TGCATCACGTTGAACGTTGA+AGG - tig0002256:173-192 None:intergenic 45.0%
CCCAAGAAGGTTCTTATCTC+TGG + tig0002256:212-231 Msa1338520:five_prime_UTR 45.0%
CCCAGAGATAAGAACCTTCT+TGG - tig0002256:216-235 None:intergenic 45.0%
TGTACGCATGTATGGATCGA+TGG - tig0002256:397-416 None:intergenic 45.0%
!! ACAGTGGGAGTAATGGATCT+TGG + tig0002256:724-743 Msa1338520:exon 45.0%
AATGGATCTTGGAGGTGGAT+CGG + tig0002256:735-754 Msa1338520:exon 45.0%
!! CTTTGGTAGAGAAGCATCTC+GGG + tig0002256:977-996 Msa1338520:CDS 45.0%
! ATCTCGGGCAGAGATTTTGA+AGG + tig0002256:992-1011 Msa1338520:CDS 45.0%
CCAGCTAAAATGCAAGGGTT+AGG - tig0002256:1032-1051 None:intergenic 45.0%
!! CCTAACCCTTGCATTTTAGC+TGG + tig0002256:1029-1048 Msa1338520:CDS 45.0%
TCGGTATCTGACCTAACGAT+CGG - tig0002256:1196-1215 None:intergenic 45.0%
! TAAAACTGGCTCCAGAAGCA+GGG - tig0002256:1355-1374 None:intergenic 45.0%
! TTAAAACTGGCTCCAGAAGC+AGG - tig0002256:1356-1375 None:intergenic 45.0%
! TTTGGTGGAATTTGGAGTGG+TGG + tig0002256:1443-1462 Msa1338520:CDS 45.0%
GGACGAAGTGTGAGACTATA+AGG - tig0002256:1673-1692 None:intergenic 45.0%
!! GTGTTGCTCGTTGATGGATT+TGG + tig0002256:1820-1839 Msa1338520:CDS 45.0%
CCATTGCAAGAGATTACAGC+GGG + tig0002256:1945-1964 Msa1338520:CDS 45.0%
ATTCTCTTGTGGAAGCTGCA+TGG + tig0002256:1988-2007 Msa1338520:CDS 45.0%
GCTTCTACAGCATTTCCTAG+AGG - tig0002256:2014-2033 None:intergenic 45.0%
CGGAGGGAGTAGTTCAATAA+TGG + tig0002256:46-65 Msa1338520:five_prime_UTR 45.0%
TGCATCACGTTGAACGTTGA+AGG - tig0002256:173-192 None:intergenic 45.0%
CCCAAGAAGGTTCTTATCTC+TGG + tig0002256:212-231 Msa1338520:five_prime_UTR 45.0%
CCCAGAGATAAGAACCTTCT+TGG - tig0002256:216-235 None:intergenic 45.0%
TGTACGCATGTATGGATCGA+TGG - tig0002256:397-416 None:intergenic 45.0%
!! ACAGTGGGAGTAATGGATCT+TGG + tig0002256:724-743 Msa1338520:exon 45.0%
AATGGATCTTGGAGGTGGAT+CGG + tig0002256:735-754 Msa1338520:exon 45.0%
!! CTTTGGTAGAGAAGCATCTC+GGG + tig0002256:977-996 Msa1338520:CDS 45.0%
! ATCTCGGGCAGAGATTTTGA+AGG + tig0002256:992-1011 Msa1338520:CDS 45.0%
CCAGCTAAAATGCAAGGGTT+AGG - tig0002256:1032-1051 None:intergenic 45.0%
!! CCTAACCCTTGCATTTTAGC+TGG + tig0002256:1029-1048 Msa1338520:CDS 45.0%
TCGGTATCTGACCTAACGAT+CGG - tig0002256:1196-1215 None:intergenic 45.0%
! TAAAACTGGCTCCAGAAGCA+GGG - tig0002256:1355-1374 None:intergenic 45.0%
! TTAAAACTGGCTCCAGAAGC+AGG - tig0002256:1356-1375 None:intergenic 45.0%
! TTTGGTGGAATTTGGAGTGG+TGG + tig0002256:1443-1462 Msa1338520:CDS 45.0%
GGACGAAGTGTGAGACTATA+AGG - tig0002256:1673-1692 None:intergenic 45.0%
!! GTGTTGCTCGTTGATGGATT+TGG + tig0002256:1820-1839 Msa1338520:CDS 45.0%
CCATTGCAAGAGATTACAGC+GGG + tig0002256:1945-1964 Msa1338520:CDS 45.0%
ATTCTCTTGTGGAAGCTGCA+TGG + tig0002256:1988-2007 Msa1338520:CDS 45.0%
GCTTCTACAGCATTTCCTAG+AGG - tig0002256:2014-2033 None:intergenic 45.0%
!! TTAAAAATAAAAAACTAATA+CGG + tig0002256:1219-1238 Msa1338520:intron 5.0%
!! TTAAAAATAAAAAACTAATA+CGG + tig0002256:1219-1238 Msa1338520:intron 5.0%
ATGGGTCATCTATTGCGGGA+CGG + tig0002256:26-45 Msa1338520:five_prime_UTR 50.0%
!! GTGGGAGTAATGGATCTTGG+AGG + tig0002256:727-746 Msa1338520:exon 50.0%
!! GGAGTAATGGATCTTGGAGG+TGG + tig0002256:730-749 Msa1338520:exon 50.0%
! TGGAGGTGGATCGGTTCAAA+TGG + tig0002256:744-763 Msa1338520:CDS 50.0%
TCTCGGTTTAGCCGATCGTT+AGG + tig0002256:1182-1201 Msa1338520:intron 50.0%
! AAAACTGGCTCCAGAAGCAG+GGG - tig0002256:1354-1373 None:intergenic 50.0%
TAGTCTCACACTTCGTCCTG+TGG + tig0002256:1675-1694 Msa1338520:CDS 50.0%
GCTTCAGTCTCAAGATCCAC+AGG - tig0002256:1694-1713 None:intergenic 50.0%
! CAGTACGTGTTGCTCGTTGA+TGG + tig0002256:1814-1833 Msa1338520:CDS 50.0%
CCCGCTGTAATCTCTTGCAA+TGG - tig0002256:1948-1967 None:intergenic 50.0%
ATGGGTCATCTATTGCGGGA+CGG + tig0002256:26-45 Msa1338520:five_prime_UTR 50.0%
!! GTGGGAGTAATGGATCTTGG+AGG + tig0002256:727-746 Msa1338520:exon 50.0%
!! GGAGTAATGGATCTTGGAGG+TGG + tig0002256:730-749 Msa1338520:exon 50.0%
! TGGAGGTGGATCGGTTCAAA+TGG + tig0002256:744-763 Msa1338520:CDS 50.0%
TCTCGGTTTAGCCGATCGTT+AGG + tig0002256:1182-1201 Msa1338520:intron 50.0%
! AAAACTGGCTCCAGAAGCAG+GGG - tig0002256:1354-1373 None:intergenic 50.0%
TAGTCTCACACTTCGTCCTG+TGG + tig0002256:1675-1694 Msa1338520:CDS 50.0%
GCTTCAGTCTCAAGATCCAC+AGG - tig0002256:1694-1713 None:intergenic 50.0%
! CAGTACGTGTTGCTCGTTGA+TGG + tig0002256:1814-1833 Msa1338520:CDS 50.0%
CCCGCTGTAATCTCTTGCAA+TGG - tig0002256:1948-1967 None:intergenic 50.0%
GTCATCTATTGCGGGACGGA+GGG + tig0002256:30-49 Msa1338520:five_prime_UTR 55.0%
! GGTGGAATTTGGAGTGGTGG+AGG + tig0002256:1446-1465 Msa1338520:CDS 55.0%
! GGAATTTGGAGTGGTGGAGG+AGG + tig0002256:1449-1468 Msa1338520:CDS 55.0%
GTCATCTATTGCGGGACGGA+GGG + tig0002256:30-49 Msa1338520:five_prime_UTR 55.0%
! GGTGGAATTTGGAGTGGTGG+AGG + tig0002256:1446-1465 Msa1338520:CDS 55.0%
! GGAATTTGGAGTGGTGGAGG+AGG + tig0002256:1449-1468 Msa1338520:CDS 55.0%
GGTCATCTATTGCGGGACGG+AGG + tig0002256:29-48 Msa1338520:five_prime_UTR 60.0%
AAGGCCAATGCCCCTGCTTC+TGG + tig0002256:1341-1360 Msa1338520:CDS 60.0%
! TGGAGTGGTGGAGGAGGAAG+TGG + tig0002256:1455-1474 Msa1338520:CDS 60.0%
GTGGAAGCTGCATGGCCTCT+AGG + tig0002256:1996-2015 Msa1338520:CDS 60.0%
GGTCATCTATTGCGGGACGG+AGG + tig0002256:29-48 Msa1338520:five_prime_UTR 60.0%
AAGGCCAATGCCCCTGCTTC+TGG + tig0002256:1341-1360 Msa1338520:CDS 60.0%
! TGGAGTGGTGGAGGAGGAAG+TGG + tig0002256:1455-1474 Msa1338520:CDS 60.0%
GTGGAAGCTGCATGGCCTCT+AGG + tig0002256:1996-2015 Msa1338520:CDS 60.0%
! GGCTCCAGAAGCAGGGGCAT+TGG - tig0002256:1348-1367 None:intergenic 65.0%
! GGCTCCAGAAGCAGGGGCAT+TGG - tig0002256:1348-1367 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0002256 gene 7 2329 7 ID=Msa1338520;Name=Msa1338520
tig0002256 mRNA 7 2329 7 ID=Msa1338520-mRNA-1;Parent=Msa1338520;Name=Msa1338520-mRNA-1;_AED=0.38;_eAED=0.38;_QI=288|1|1|1|1|1|6|250|263
tig0002256 exon 7 234 7 ID=Msa1338520-mRNA-1:exon:1695;Parent=Msa1338520-mRNA-1
tig0002256 exon 676 881 676 ID=Msa1338520-mRNA-1:exon:1696;Parent=Msa1338520-mRNA-1
tig0002256 exon 968 1059 968 ID=Msa1338520-mRNA-1:exon:1697;Parent=Msa1338520-mRNA-1
tig0002256 exon 1312 1526 1312 ID=Msa1338520-mRNA-1:exon:1698;Parent=Msa1338520-mRNA-1
tig0002256 exon 1646 1841 1646 ID=Msa1338520-mRNA-1:exon:1699;Parent=Msa1338520-mRNA-1
tig0002256 exon 1937 2329 1937 ID=Msa1338520-mRNA-1:exon:1700;Parent=Msa1338520-mRNA-1
tig0002256 five_prime_UTR 7 234 7 ID=Msa1338520-mRNA-1:five_prime_utr;Parent=Msa1338520-mRNA-1
tig0002256 five_prime_UTR 676 735 676 ID=Msa1338520-mRNA-1:five_prime_utr;Parent=Msa1338520-mRNA-1
tig0002256 CDS 736 881 736 ID=Msa1338520-mRNA-1:cds;Parent=Msa1338520-mRNA-1
tig0002256 CDS 968 1059 968 ID=Msa1338520-mRNA-1:cds;Parent=Msa1338520-mRNA-1
tig0002256 CDS 1312 1526 1312 ID=Msa1338520-mRNA-1:cds;Parent=Msa1338520-mRNA-1
tig0002256 CDS 1646 1841 1646 ID=Msa1338520-mRNA-1:cds;Parent=Msa1338520-mRNA-1
tig0002256 CDS 1937 2079 1937 ID=Msa1338520-mRNA-1:cds;Parent=Msa1338520-mRNA-1
tig0002256 three_prime_UTR 2080 2329 2080 ID=Msa1338520-mRNA-1:three_prime_utr;Parent=Msa1338520-mRNA-1
Gene Sequence

>Msa1338520

ATGGATCTTGGAGGTGGATCGGTTCAAATGGCATATGCAGTGTCAAGGAATACAGCTAAAAATGCTCCAAAAGTTGCAGATGGAGAAGATCCATACATTAAGAAGCTTGTACTCAAGGGAAAAAAATATGATCTCTATGTTCATAGTTACTTACACTTTGGTAGAGAAGCATCTCGGGCAGAGATTTTGAAGGTCACTCATAATTCTCCTAACCCTTGCATTTTAGCTGGATTTGATGGGACATACACATATGCTGGAGAAGAATTTAAGGCCAATGCCCCTGCTTCTGGAGCCAGTTTTAAAAAATGCAAAAAGATAGTTCGTCAGGCACTTAAATTGAACTATCCATGTCCTTATCAGAATTGCACTTTTGGTGGAATTTGGAGTGGTGGAGGAGGAAGTGGACAGAGAATACTTTTTGCTGCTTCATCTTTCTTTTACCTAGCTGAAGATATTGGTTTGGTTGACCCAAATACACCTTATAGTCTCACACTTCGTCCTGTGGATCTTGAGACTGAAGCTAAGAGAGCTTGTACATTAAATCTTGAGGAGGCCAAATCAACTTATCCACTTCTTGTGGATTTCAATTTAGTAGAATATGTTTGCATGGATCTTATATATCAGTACGTGTTGCTCGTTGATGGATTTGGCTTAGATCCATTGCAAGAGATTACAGCGGGAAAGCAAATTGAATATCAAGATTCTCTTGTGGAAGCTGCATGGCCTCTAGGAAATGCTGTAGAAGCCATATCATCGTTACCTAAATTTGAACGATTGATGTATTTTATTTAA
Protein sequence

>Msa1338520

MDLGGGSVQMAYAVSRNTAKNAPKVADGEDPYIKKLVLKGKKYDLYVHSYLHFGREASRAEILKVTHNSPNPCILAGFDGTYTYAGEEFKANAPASGASFKKCKKIVRQALKLNYPCPYQNCTFGGIWSGGGGSGQRILFAASSFFYLAEDIGLVDPNTPYSLTLRPVDLETEAKRACTLNLEEAKSTYPLLVDFNLVEYVCMDLIYQYVLLVDGFGLDPLQEITAGKQIEYQDSLVEAAWPLGNAVEAISSLPKFERLMYFI*