AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1169640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1169640 MtrunA17_Chr7g0263581 97.877 471 10 0 1 471 1 471 0.0 965
Msa1169640 MtrunA17_Chr4g0030191 54.503 433 134 13 1 408 1 395 3.21e-118 354
Msa1169640 MtrunA17_Chr2g0325911 50.808 433 139 16 1 408 1 384 3.78e-103 315
Msa1169640 MtrunA17_Chr5g0401891 81.313 198 35 1 117 314 132 327 3.84e-96 299
Msa1169640 MtrunA17_Chr4g0053181 82.812 192 32 1 124 314 161 352 1.07e-93 293
Msa1169640 MtrunA17_Chr7g0216601 50.000 202 87 3 136 323 65 266 5.05e-55 188
Msa1169640 MtrunA17_Chr1g0170761 43.161 329 141 13 1 315 3 299 2.82e-54 186
Msa1169640 MtrunA17_Chr4g0072291 49.231 195 87 4 110 293 134 327 1.78e-50 179
Msa1169640 MtrunA17_Chr3g0126151 80.851 94 18 0 177 270 47 140 5.11e-50 167
Msa1169640 MtrunA17_Chr4g0054351 52.047 171 71 3 136 295 310 480 6.38e-50 180
Msa1169640 MtrunA17_Chr4g0054351 43.590 78 42 1 125 200 401 478 9.18e-12 67.4
Msa1169640 MtrunA17_Chr4g0032631 52.071 169 70 3 136 293 163 331 2.92e-49 176
Msa1169640 MtrunA17_Chr4g0032631 44.156 77 41 1 126 200 255 331 5.59e-12 67.8
Msa1169640 MtrunA17_Chr4g0032631 42.857 91 43 2 215 296 150 240 7.35e-11 64.3
Msa1169640 MtrunA17_Chr7g0249621 45.631 206 93 4 136 330 259 456 2.94e-49 179
Msa1169640 MtrunA17_Chr4g0002011 49.770 217 84 6 130 321 30 246 7.82e-49 170
Msa1169640 MtrunA17_Chr5g0400871 51.462 171 72 3 136 295 249 419 7.91e-49 175
Msa1169640 MtrunA17_Chr8g0355191 45.327 214 101 4 118 323 37 242 1.15e-48 172
Msa1169640 MtrunA17_Chr4g0073521 46.809 188 92 3 113 292 24 211 6.95e-47 166
Msa1169640 MtrunA17_Chr3g0135161 51.190 168 74 3 136 295 310 477 7.03e-47 172
Msa1169640 MtrunA17_Chr8g0343851 51.807 166 72 3 136 293 248 413 7.04e-47 171
Msa1169640 MtrunA17_Chr1g0152161 50.595 168 75 3 136 295 312 479 3.09e-46 170
Msa1169640 MtrunA17_Chr6g0452911 48.619 181 75 4 140 302 65 245 9.84e-46 163
Msa1169640 MtrunA17_Chr8g0355181 47.236 199 93 4 119 307 37 233 1.01e-44 160
Msa1169640 MtrunA17_Chr2g0328971 49.704 169 71 4 136 293 167 332 1.31e-44 163
Msa1169640 MtrunA17_Chr2g0328971 44.156 77 41 1 126 200 256 332 1.04e-11 67.0
Msa1169640 MtrunA17_Chr5g0412151 51.053 190 82 3 117 295 205 394 3.05e-44 162
Msa1169640 MtrunA17_Chr8g0367311 56.716 134 57 1 163 295 19 152 8.26e-43 152
Msa1169640 MtrunA17_Chr8g0367311 36.449 107 63 3 102 203 48 154 1.54e-11 64.7
Msa1169640 MtrunA17_Chr8g0362541 48.603 179 70 3 136 292 69 247 2.81e-38 144
Msa1169640 MtrunA17_Chr7g0257361 48.571 140 55 3 136 262 57 192 1.45e-25 108
Msa1169640 MtrunA17_Chr2g0305821 44.697 132 64 3 116 238 25 156 1.46e-20 88.6
Msa1169640 MtrunA17_Chr2g0305831 50.000 66 33 0 238 303 5 70 3.26e-13 68.6
Msa1169640 MtrunA17_Chr8g0375031 53.125 64 27 2 139 200 124 186 7.92e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1169640 AT2G28550.3 53.440 436 152 15 1 408 1 413 1.50e-109 332
Msa1169640 AT4G36920.1 75.814 215 48 2 117 328 110 323 3.64e-95 294
Msa1169640 AT4G36920.2 75.814 215 48 2 117 328 110 323 3.64e-95 294
Msa1169640 AT2G28550.1 50.459 436 150 16 1 408 1 398 5.07e-95 295
Msa1169640 AT5G67180.2 75.000 204 47 1 111 314 72 271 2.36e-90 277
Msa1169640 AT5G67180.1 75.000 204 47 1 111 314 72 271 3.01e-90 279
Msa1169640 AT5G67180.3 80.000 185 33 1 111 295 72 252 2.36e-88 271
Msa1169640 AT2G28550.4 58.147 313 98 8 1 289 1 304 9.06e-87 270
Msa1169640 AT2G28550.2 60.289 277 78 7 1 254 1 268 1.59e-76 241
Msa1169640 AT2G28550.6 60.289 277 78 7 1 254 1 268 1.59e-76 241
Msa1169640 AT5G67180.4 72.826 184 43 3 111 293 72 249 1.26e-73 233
Msa1169640 AT5G60120.1 38.171 503 219 18 1 455 1 459 9.57e-70 230
Msa1169640 AT5G60120.6 38.431 497 219 18 1 455 1 452 2.47e-69 229
Msa1169640 AT5G60120.4 43.836 365 139 12 1 330 1 334 4.51e-66 218
Msa1169640 AT5G60120.3 43.836 365 139 12 1 330 1 334 4.51e-66 218
Msa1169640 AT5G60120.5 43.836 365 139 12 1 330 1 334 4.51e-66 218
Msa1169640 AT5G60120.2 37.143 525 216 20 1 455 1 481 5.95e-66 221
Msa1169640 AT2G28550.5 52.713 258 88 9 156 408 4 232 7.78e-64 208
Msa1169640 AT1G72570.1 48.958 192 85 4 115 293 197 388 8.15e-52 181
Msa1169640 AT4G37750.1 43.460 237 107 4 114 323 258 494 5.89e-51 182
Msa1169640 AT1G51190.1 43.777 233 104 6 72 293 139 355 8.24e-51 181
Msa1169640 AT5G57390.2 48.168 191 81 4 121 293 60 250 3.02e-50 177
Msa1169640 AT5G57390.1 48.168 191 81 4 121 293 178 368 1.02e-49 178
Msa1169640 AT2G39250.1 39.701 335 123 11 1 326 1 265 3.05e-49 171
Msa1169640 AT3G20840.1 50.888 169 72 3 136 293 178 346 6.94e-49 176
Msa1169640 AT3G20840.1 44.156 77 41 1 126 200 270 346 8.78e-12 67.8
Msa1169640 AT5G17430.2 48.454 194 84 5 112 292 122 312 1.07e-48 175
Msa1169640 AT5G17430.1 48.454 194 84 5 112 292 184 374 2.00e-48 175
Msa1169640 AT2G39250.2 46.473 241 82 6 1 233 1 202 3.13e-48 166
Msa1169640 AT3G54320.3 46.829 205 84 4 120 299 32 236 8.89e-45 162
Msa1169640 AT3G54320.1 46.602 206 85 4 120 300 32 237 1.28e-44 162
Msa1169640 AT5G65510.4 54.386 171 67 3 136 295 170 340 2.61e-44 161
Msa1169640 AT5G65510.2 54.386 171 67 3 136 295 228 398 4.33e-44 162
Msa1169640 AT5G65510.1 54.386 171 67 3 136 295 228 398 4.33e-44 162
Msa1169640 AT3G54990.2 61.818 165 49 3 84 245 75 228 5.67e-44 155
Msa1169640 AT3G54990.1 59.016 183 50 3 97 279 91 248 9.65e-44 157
Msa1169640 AT5G10510.7 52.047 171 71 3 136 295 227 397 3.23e-42 157
Msa1169640 AT5G10510.5 52.047 171 71 3 136 295 227 397 3.23e-42 157
Msa1169640 AT5G10510.6 52.047 171 71 3 136 295 265 435 4.24e-42 158
Msa1169640 AT5G10510.4 52.047 171 71 3 136 295 265 435 4.24e-42 158
Msa1169640 AT5G10510.1 52.047 171 71 3 136 295 250 420 4.53e-42 157
Msa1169640 AT5G65510.3 53.191 141 55 3 136 265 170 310 2.35e-40 147
Msa1169640 AT1G79700.2 53.448 174 70 2 130 292 43 216 4.39e-40 147
Msa1169640 AT1G16060.1 50.000 188 83 2 116 292 35 222 1.16e-39 146
Msa1169640 AT5G10510.2 50.000 168 76 3 136 295 227 394 4.60e-38 146
Msa1169640 AT5G10510.3 45.026 191 74 4 136 295 265 455 1.95e-35 139
Msa1169640 AT1G79700.1 50.575 174 65 3 130 292 43 206 7.79e-33 127
Msa1169640 AT3G54320.4 50.725 138 67 1 163 299 33 170 1.39e-31 125
Msa1169640 AT3G54320.5 50.725 138 67 1 163 299 33 170 1.39e-31 125
Msa1169640 AT1G79700.3 54.412 136 61 1 158 292 5 140 1.01e-28 114
Msa1169640 AT1G16060.3 54.412 136 61 1 158 292 17 152 7.67e-28 112
Msa1169640 AT1G79700.4 54.264 129 58 1 165 292 6 134 1.43e-26 107
Msa1169640 AT2G41710.1 48.855 131 53 2 136 256 67 193 4.83e-25 107
Msa1169640 AT2G41710.4 48.855 131 53 2 136 256 67 193 6.30e-25 107
Msa1169640 AT2G41710.2 47.059 136 53 3 136 256 67 198 6.68e-23 101
Msa1169640 AT2G41710.3 46.281 121 51 2 146 256 112 228 3.41e-20 93.2
Msa1169640 AT3G54320.2 54.167 96 43 1 206 300 1 96 3.49e-19 88.2
Msa1169640 AT1G16060.2 55.682 88 38 1 206 292 1 88 7.91e-18 82.4
Msa1169640 AT1G16060.4 55.682 88 38 1 206 292 1 88 7.91e-18 82.4
Msa1169640 AT1G16060.6 55.682 88 38 1 206 292 1 88 7.91e-18 82.4
Msa1169640 AT1G16060.5 55.682 88 38 1 206 292 1 88 7.91e-18 82.4
Msa1169640 AT3G25730.1 38.947 95 54 2 128 222 53 143 2.51e-12 68.6
Msa1169640 AT1G68840.2 37.383 107 63 2 116 222 44 146 1.07e-11 66.6
Msa1169640 AT1G68840.1 37.383 107 63 2 116 222 44 146 1.07e-11 66.6
Msa1169640 AT2G47520.1 44.578 83 43 2 122 202 32 113 1.37e-11 63.5

Find 165 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAGCTGCCATGCCCAATT+TGG 0.214682 7_4:-62665929 None:intergenic
CTCAATGTTCAGTCATCTTA+TGG 0.249805 7_4:+62665672 Msa1169640:CDS
GTTTGCAAGTTCAGCGTAAC+TGG 0.251291 7_4:+62665469 Msa1169640:CDS
GTCATTCCCGATTCGTTTGC+TGG 0.257226 7_4:-62663732 None:intergenic
AATCTGCTCCCGTTCCAAAT+TGG 0.265423 7_4:+62665915 Msa1169640:CDS
CTCCAAAGTCCATATAATAA+TGG 0.282974 7_4:+62665947 Msa1169640:CDS
GACGTTGGGAATCGCATATT+TGG 0.285512 7_4:+62663925 Msa1169640:CDS
AGTCTTTGTATCCAAGGTTT+AGG 0.309744 7_4:+62666193 Msa1169640:three_prime_UTR
AAAGTTGACCCATTATTATA+TGG 0.312812 7_4:-62665956 None:intergenic
GCTCGTATGGGACAGTTTCT+TGG 0.324940 7_4:+62664725 Msa1169640:CDS
GGAAGCGTTGTTGTTGAGTA+TGG 0.328168 7_4:-62663631 None:intergenic
ACGATTCAGTTATGAAGATT+TGG 0.335301 7_4:-62663435 None:intergenic
ATATAACCCACAAATAATAT+TGG 0.339629 7_4:-62666527 None:intergenic
ATCTGCTCCCGTTCCAAATT+GGG 0.340346 7_4:+62665916 Msa1169640:CDS
CGTTGATAGTTTGAGGACTC+TGG 0.357542 7_4:+62665639 Msa1169640:intron
AATGACATTATATTGCTATA+AGG 0.366223 7_4:+62663270 Msa1169640:five_prime_UTR
AAGAAAATAGGAATATTTAA+AGG 0.373877 7_4:-62666293 None:intergenic
ACCCATTATTATATGGACTT+TGG 0.377342 7_4:-62665949 None:intergenic
TTATGTAGGTATATATATCT+TGG 0.380122 7_4:+62664887 Msa1169640:intron
TTCTCTGCTGCAGCATCATC+AGG 0.380972 7_4:+62665992 Msa1169640:CDS
CTGAGCATCAACCTCCAATC+TGG 0.386362 7_4:+62665709 Msa1169640:CDS
AAAGTAGGAATTTAATTGCT+TGG 0.388624 7_4:+62666244 Msa1169640:three_prime_UTR
ACCATTACACTCTTATCAAC+AGG 0.390876 7_4:-62665501 None:intergenic
GTTACTTTCTACCGGAGAAC+TGG 0.391369 7_4:+62663903 Msa1169640:CDS
TCCAAAGTCCATATAATAAT+GGG 0.393361 7_4:+62665948 Msa1169640:CDS
GATGCTGCAGCAGAGAAATT+AGG 0.402479 7_4:-62665986 None:intergenic
TAATTACTTTCCCGTGTGTA+AGG 0.413461 7_4:+62665740 Msa1169640:CDS
CTCGTATGGGACAGTTTCTT+GGG 0.413776 7_4:+62664726 Msa1169640:CDS
TATGTAGGTATATATATCTT+GGG 0.414146 7_4:+62664888 Msa1169640:intron
AATATGATCATCAAACTTGT+AGG 0.417027 7_4:+62666216 Msa1169640:three_prime_UTR
TGCCATGCCCAATTTGGAAC+GGG 0.426003 7_4:-62665923 None:intergenic
GAATTGTAGCTTTATACTCT+AGG 0.434066 7_4:-62666151 None:intergenic
ACTGAAAATATTTGAGATGA+TGG 0.440420 7_4:+62666441 Msa1169640:three_prime_UTR
TTCTTCACCTGCTGAGGTCG+TGG 0.440742 7_4:-62663837 None:intergenic
GGGTGGAGGTATGTTATGTT+TGG 0.445569 7_4:-62666055 None:intergenic
AGTTTACCATGCCTTGCGGC+AGG 0.447162 7_4:-62666332 None:intergenic
ATGATAACAAAGGAAGGAAA+TGG 0.447543 7_4:-62666396 None:intergenic
ATTTGAACATCAGAGTTAGA+AGG 0.449742 7_4:-62665420 None:intergenic
TTAAATTCCAATATTATTTG+TGG 0.449904 7_4:+62666520 Msa1169640:three_prime_UTR
TAAATTCCAATATTATTTGT+GGG 0.457494 7_4:+62666521 Msa1169640:three_prime_UTR
TGGACGATGGGAAGCTCGTA+TGG 0.459668 7_4:+62664712 Msa1169640:CDS
ATGATCATATTCCTAAACCT+TGG 0.459978 7_4:-62666204 None:intergenic
CCTGCAGTAGAAAGAGGGTA+TGG 0.464065 7_4:-62666110 None:intergenic
TACAACAGCTTCGCCGGCGC+CGG 0.464183 7_4:-62663691 None:intergenic
GCAGAATCTTGATCTGAACT+TGG 0.465184 7_4:+62665386 Msa1169640:CDS
CTATCCGCAGCTGTTGTTGA+AGG 0.465186 7_4:-62666019 None:intergenic
AAGAGCAATAGAGAATAGGA+TGG 0.477231 7_4:+62665892 Msa1169640:CDS
ACTCTCCTCCGTCGTTCAGA+TGG 0.481608 7_4:+62663515 Msa1169640:CDS
CATATTCACGAGAACAATGA+AGG 0.482184 7_4:+62663334 Msa1169640:five_prime_UTR
CTACCGGAGAACTGGACGTT+GGG 0.483697 7_4:+62663911 Msa1169640:CDS
AGGAAAGAGCAATAGAGAAT+AGG 0.487980 7_4:+62665888 Msa1169640:intron
CCATACCCTCTTTCTACTGC+AGG 0.495357 7_4:+62666110 Msa1169640:CDS
TTTGAGGCTAGTTCTTATGA+AGG 0.495376 7_4:+62665127 Msa1169640:CDS
ACTGAAGATTCATGCTCTTA+CGG 0.496241 7_4:+62663585 Msa1169640:CDS
TAGTACCATCGTTATCAGCC+TGG 0.496630 7_4:-62665162 None:intergenic
TGCTCTTACGGCGATGCATA+CGG 0.502837 7_4:+62663597 Msa1169640:CDS
TGTTGCTCCACGACCTCAGC+AGG 0.503437 7_4:+62663830 Msa1169640:CDS
TTTATGAGATGATTCTAATG+AGG 0.504275 7_4:+62666370 Msa1169640:three_prime_UTR
ACGTCTAGTCTTTGTATCCA+AGG 0.505412 7_4:+62666187 Msa1169640:three_prime_UTR
TGTCCGTACAACAGCTTCGC+CGG 0.508835 7_4:-62663697 None:intergenic
GTAGAAAGAGGGTATGGGGC+TGG 0.510625 7_4:-62666104 None:intergenic
ATCGTGGTGTTACTTTCTAC+CGG 0.518398 7_4:+62663895 Msa1169640:CDS
TAATTAGTACCACTCCAGAT+TGG 0.520137 7_4:-62665723 None:intergenic
AACATGCACCACAGTGGAAG+TGG 0.520906 7_4:+62665447 Msa1169640:CDS
ATTCCCAACGTCCAGTTCTC+CGG 0.521427 7_4:-62663914 None:intergenic
ATGGGGCTGGAGTTAGTCGC+TGG 0.523340 7_4:-62666091 None:intergenic
TGAAGGAGAGTTAACTTCCC+AGG 0.523464 7_4:+62665144 Msa1169640:CDS
ATTCAGTTATGAAGATTTGG+AGG 0.523564 7_4:-62663432 None:intergenic
GTCGCTGGAGTAGGAAAATG+AGG 0.525924 7_4:-62666076 None:intergenic
GAGCTTCAACACTCCGGCGC+CGG 0.528911 7_4:+62663678 Msa1169640:CDS
CGGTACGCCACCGTGATGAT+CGG 0.529586 7_4:-62663793 None:intergenic
AGGTTGATGCTCAGATGCTA+TGG 0.530023 7_4:-62665700 None:intergenic
TGTGGAAAACAAGTATACTT+AGG 0.532079 7_4:+62664063 Msa1169640:CDS
CTGCTATAAAATGCAATGGA+AGG 0.535486 7_4:+62665089 Msa1169640:CDS
CTGCAGTAGAAAGAGGGTAT+GGG 0.539113 7_4:-62666109 None:intergenic
CGATCATCACGGTGGCGTAC+CGG 0.539554 7_4:+62663794 Msa1169640:CDS
GTGAAAATGATAACAAAGGA+AGG 0.539785 7_4:-62666402 None:intergenic
CTGGAGTTAGTCGCTGGAGT+AGG 0.547085 7_4:-62666085 None:intergenic
AAACTCAAACCTCACAATGT+TGG 0.548139 7_4:+62663461 Msa1169640:exon
GCCGGAGTGTTGAAGCTCAC+CGG 0.549298 7_4:-62663673 None:intergenic
GTTGATAGTTTGAGGACTCT+GGG 0.550553 7_4:+62665640 Msa1169640:intron
GACAGAGTACTGGTTTCTCA+AGG 0.553049 7_4:+62664651 Msa1169640:CDS
GGTAAGAAGAGGAATAGAAG+AGG 0.553422 7_4:+62663291 Msa1169640:five_prime_UTR
GTTTACCATGCCTTGCGGCA+GGG 0.553482 7_4:-62666331 None:intergenic
TCAGCTCCTGCAGTAGAAAG+AGG 0.553716 7_4:-62666116 None:intergenic
TGATTACAGAGCGTATGATA+AGG 0.555672 7_4:+62665066 Msa1169640:intron
CTCCCGTTCCAAATTGGGCA+TGG 0.556092 7_4:+62665921 Msa1169640:CDS
TTATGATGAAGATATAAAGC+AGG 0.557521 7_4:+62664398 Msa1169640:CDS
GCGCCGGCGAAGCTGTTGTA+CGG 0.557964 7_4:+62663694 Msa1169640:CDS
TACTGCGAGCTTCGTGACCT+CGG 0.565421 7_4:-62663873 None:intergenic
GATTGAGATCCAACATTGTG+AGG 0.565610 7_4:-62663470 None:intergenic
AAAATCCCTGCCGCAAGGCA+TGG 0.567862 7_4:+62666326 Msa1169640:three_prime_UTR
ATCTGAACGACGGAGGAGAG+TGG 0.568541 7_4:-62663513 None:intergenic
ATATGATCATCAAACTTGTA+GGG 0.568850 7_4:+62666217 Msa1169640:three_prime_UTR
AACTTGTAGGGTCTAAAAGT+AGG 0.569140 7_4:+62666229 Msa1169640:three_prime_UTR
ATTCTGCGTAGACAGAGTAC+TGG 0.571541 7_4:+62664641 Msa1169640:CDS
GAGTAGGAAAATGAGGGTGG+AGG 0.571882 7_4:-62666069 None:intergenic
GTTCAGATGGAAGATTCATC+CGG 0.575771 7_4:+62663528 Msa1169640:CDS
CGAGCTGCGATAAAGTTTCG+TGG 0.575785 7_4:+62664342 Msa1169640:CDS
TGCAGTAGAAAGAGGGTATG+GGG 0.576798 7_4:-62666108 None:intergenic
TATCCGCAGCTGTTGTTGAA+GGG 0.577107 7_4:-62666018 None:intergenic
TGCTATAAAATGCAATGGAA+GGG 0.577688 7_4:+62665090 Msa1169640:CDS
CGCCTTCTGAGAGTAGAGGA+TGG 0.578820 7_4:+62663757 Msa1169640:CDS
GTCGACAACGGTACTATCTC+CGG 0.580552 7_4:+62663654 Msa1169640:CDS
TTACCTTGCCGCAGCATGCG+CGG 0.580853 7_4:-62664211 None:intergenic
TTCAGTTATGAAGATTTGGA+GGG 0.581412 7_4:-62663431 None:intergenic
TCTACCGGAGAACTGGACGT+TGG 0.583316 7_4:+62663910 Msa1169640:CDS
ATACCGCGCATGCTGCGGCA+AGG 0.583419 7_4:+62664208 Msa1169640:CDS
GCAACAACAACAGTCTGCTC+CGG 0.583622 7_4:-62663813 None:intergenic
CAGAATCTTGATCTGAACTT+GGG 0.584312 7_4:+62665387 Msa1169640:CDS
AAGGCTGCTATAAAATGCAA+TGG 0.584344 7_4:+62665085 Msa1169640:CDS
TTAGTACCACTCCAGATTGG+AGG 0.585536 7_4:-62665720 None:intergenic
GAATGAAAGATAGAGAGAGA+AGG 0.588114 7_4:-62663377 None:intergenic
GACGGAGGAATCAAAGCTAC+CGG 0.588222 7_4:-62663547 None:intergenic
TTTGCAAGTTCAGCGTAACT+GGG 0.588454 7_4:+62665470 Msa1169640:CDS
TCTGCATAAATGTGGACGAT+GGG 0.589018 7_4:+62664700 Msa1169640:CDS
AGTACCATCGTTATCAGCCT+GGG 0.589962 7_4:-62665161 None:intergenic
ATCCATCCTCTACTCTCAGA+AGG 0.594152 7_4:-62663759 None:intergenic
CTGCCATGCCCAATTTGGAA+CGG 0.595613 7_4:-62665924 None:intergenic
GACGGAGGAGAGTGGAAGAG+AGG 0.597615 7_4:-62663505 None:intergenic
ATAGCGAGCTAGATGCTGCA+AGG 0.598807 7_4:+62664918 Msa1169640:CDS
ACAGAGTACTGGTTTCTCAA+GGG 0.599902 7_4:+62664652 Msa1169640:CDS
ATTTGATACCGCGCATGCTG+CGG 0.600999 7_4:+62664203 Msa1169640:CDS
TCCTCTACTCTCAGAAGGCG+AGG 0.602345 7_4:-62663754 None:intergenic
CAGAGTACTGGTTTCTCAAG+GGG 0.605158 7_4:+62664653 Msa1169640:CDS
TATTGCTATAAGGTAAGAAG+AGG 0.605217 7_4:+62663280 Msa1169640:five_prime_UTR
AAAGTTTCGTGGCGTCGATG+CGG 0.606313 7_4:+62664353 Msa1169640:CDS
CGACTCTTCTTCACCTGCTG+AGG 0.608124 7_4:-62663843 None:intergenic
GGACGATGGGAAGCTCGTAT+GGG 0.611195 7_4:+62664713 Msa1169640:CDS
CAGCTCCTGCAGTAGAAAGA+GGG 0.613397 7_4:-62666115 None:intergenic
ATTAGTATTCACCTTACACA+CGG 0.613966 7_4:-62665751 None:intergenic
TGAACATCAGAGTTAGAAGG+AGG 0.614498 7_4:-62665417 None:intergenic
GCCTGTTGATAAGAGTGTAA+TGG 0.619307 7_4:+62665500 Msa1169640:CDS
AATTTCTCGTCCGATCATCA+CGG 0.619679 7_4:+62663783 Msa1169640:CDS
AACAACGCTTCCGTCGACAA+CGG 0.620133 7_4:+62663642 Msa1169640:CDS
CTAGAGTTTACCATGCCTTG+CGG 0.621770 7_4:-62666336 None:intergenic
GGAGTTACTCTGCATAAATG+TGG 0.624873 7_4:+62664692 Msa1169640:CDS
CTCTGCATAAATGTGGACGA+TGG 0.629073 7_4:+62664699 Msa1169640:CDS
GAGAGTGAAAATGATAACAA+AGG 0.632471 7_4:-62666406 None:intergenic
ACCTCGCCTTCTGAGAGTAG+AGG 0.632485 7_4:+62663753 Msa1169640:CDS
TCCGGTGAGCTTCAACACTC+CGG 0.632556 7_4:+62663672 Msa1169640:CDS
GTAGATGAATAACTTTACAA+AGG 0.633971 7_4:+62664604 Msa1169640:intron
TCACGAAGCTCGCAGTATCG+TGG 0.635514 7_4:+62663879 Msa1169640:CDS
AGAGCATGAATCTTCAGTGT+CGG 0.641138 7_4:-62663580 None:intergenic
GGTTGATGCTCAGATGCTAT+GGG 0.641889 7_4:-62665699 None:intergenic
GTCGGCAGCGTTGACGACGG+AGG 0.642992 7_4:-62663562 None:intergenic
AGAAGAGTCGTCGTGGACCG+AGG 0.647561 7_4:+62663856 Msa1169640:CDS
GCTGCAGCAGAGAAATTAGG+TGG 0.656992 7_4:-62665983 None:intergenic
AATATTTGAGATGATGGTGA+AGG 0.660564 7_4:+62666447 Msa1169640:three_prime_UTR
CTGGAGTAGGAAAATGAGGG+TGG 0.661562 7_4:-62666072 None:intergenic
ATATTCACGAGAACAATGAA+GGG 0.661827 7_4:+62663335 Msa1169640:five_prime_UTR
CATCAACCTCCAATCTGGAG+TGG 0.662314 7_4:+62665714 Msa1169640:CDS
GGAGATAGTACCGTTGTCGA+CGG 0.663908 7_4:-62663652 None:intergenic
ATCTTCCATCTGAACGACGG+AGG 0.664445 7_4:-62663520 None:intergenic
AGTGTCGGCAGCGTTGACGA+CGG 0.674336 7_4:-62663565 None:intergenic
TTAGTATTCACCTTACACAC+GGG 0.680796 7_4:-62665750 None:intergenic
AGGGGAAGTTCTAAATACAG+AGG 0.681558 7_4:+62664671 Msa1169640:CDS
ATTCCCTTCAACAACAGCTG+CGG 0.681754 7_4:+62666015 Msa1169640:CDS
CAGGTGAAGAAGAGTCGTCG+TGG 0.684547 7_4:+62663849 Msa1169640:CDS
TGAATCTTCCATCTGAACGA+CGG 0.693422 7_4:-62663523 None:intergenic
TCGCTGGAGTAGGAAAATGA+GGG 0.701704 7_4:-62666075 None:intergenic
TTCTCGTCCGATCATCACGG+TGG 0.718908 7_4:+62663786 Msa1169640:CDS
ACTTCCCAGGCTGATAACGA+TGG 0.728400 7_4:+62665157 Msa1169640:CDS
ATGATGAACATGCACCACAG+TGG 0.729813 7_4:+62665441 Msa1169640:CDS
CTTGCAAACCACTTCCACTG+TGG 0.732557 7_4:-62665455 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATCAAATTAAACTAA+AGG - chr7_4:62664767-62664786 None:intergenic 10.0%
!!! TATTTAGTTCTTAATTATAT+TGG + chr7_4:62664835-62664854 Msa1169640:intron 10.0%
!! AAAGATCAAAATCTAAAAAA+AGG - chr7_4:62664162-62664181 None:intergenic 15.0%
!!! TCTTTGAAATTTAATTTTTC+AGG + chr7_4:62664177-62664196 Msa1169640:intron 15.0%
!!! TAAATATTGAATGTTTTTCA+TGG + chr7_4:62664274-62664293 Msa1169640:intron 15.0%
!! TATAATTAAGAACTAAATAC+TGG - chr7_4:62664834-62664853 None:intergenic 15.0%
!!! AAATGCCTAATTAAATATTT+TGG + chr7_4:62664981-62665000 Msa1169640:intron 15.0%
!! ATGATCCAAAATATTTAATT+AGG - chr7_4:62664989-62665008 None:intergenic 15.0%
!! TGATTATAAAACCAAAAAAA+AGG - chr7_4:62665209-62665228 None:intergenic 15.0%
!! AAGAAAATAGGAATATTTAA+AGG - chr7_4:62666296-62666315 None:intergenic 15.0%
!! TTAAATTCCAATATTATTTG+TGG + chr7_4:62666520-62666539 Msa1169640:three_prime_UTR 15.0%
!! AATGACATTATATTGCTATA+AGG + chr7_4:62663270-62663289 Msa1169640:five_prime_UTR 20.0%
!!! TTGAAATTTAATTTTTCAGG+TGG + chr7_4:62664180-62664199 Msa1169640:intron 20.0%
!!! GTTTTTAATTATATTGGTCT+GGG + chr7_4:62664863-62664882 Msa1169640:intron 20.0%
!! TTATGTAGGTATATATATCT+TGG + chr7_4:62664887-62664906 Msa1169640:intron 20.0%
!! TATGTAGGTATATATATCTT+GGG + chr7_4:62664888-62664907 Msa1169640:intron 20.0%
!! ATTTATCAATCATATATAGC+AGG + chr7_4:62664946-62664965 Msa1169640:intron 20.0%
!! ATTATTTGTGTTCTATTTAC+AGG + chr7_4:62665868-62665887 Msa1169640:intron 20.0%
! TTATGATGAAGATATAAAGC+AGG + chr7_4:62664398-62664417 Msa1169640:CDS 25.0%
! GTAGATGAATAACTTTACAA+AGG + chr7_4:62664604-62664623 Msa1169640:intron 25.0%
!!! AATCACAAATCAGTCATTTT+AGG - chr7_4:62664797-62664816 None:intergenic 25.0%
!!! AAGAACTAAATACTGGTTTA+TGG - chr7_4:62664827-62664846 None:intergenic 25.0%
! AGTTCTTAATTATATTGGTC+TGG + chr7_4:62664840-62664859 Msa1169640:intron 25.0%
!! GTTCTTAATTATATTGGTCT+GGG + chr7_4:62664841-62664860 Msa1169640:intron 25.0%
!!! GTCTGGGTTTTTAATTATAT+TGG + chr7_4:62664857-62664876 Msa1169640:intron 25.0%
!!! GGTTTTTAATTATATTGGTC+TGG + chr7_4:62664862-62664881 Msa1169640:intron 25.0%
! AGGTTAATTGACATACTTTA+AGG - chr7_4:62665293-62665312 None:intergenic 25.0%
! TTAACCTTTACTATTAAGAC+TGG + chr7_4:62665306-62665325 Msa1169640:intron 25.0%
! TCCAAAGTCCATATAATAAT+GGG + chr7_4:62665948-62665967 Msa1169640:CDS 25.0%
! AAAGTTGACCCATTATTATA+TGG - chr7_4:62665959-62665978 None:intergenic 25.0%
! AATATGATCATCAAACTTGT+AGG + chr7_4:62666216-62666235 Msa1169640:three_prime_UTR 25.0%
! ATATGATCATCAAACTTGTA+GGG + chr7_4:62666217-62666236 Msa1169640:three_prime_UTR 25.0%
! AAAGTAGGAATTTAATTGCT+TGG + chr7_4:62666244-62666263 Msa1169640:three_prime_UTR 25.0%
!!! TTTTTTACACGGAAGAAAAT+AGG - chr7_4:62666308-62666327 None:intergenic 25.0%
! TTTATGAGATGATTCTAATG+AGG + chr7_4:62666370-62666389 Msa1169640:three_prime_UTR 25.0%
!! TATTTTCAGTAAGAAATGCA+TGG - chr7_4:62666431-62666450 None:intergenic 25.0%
!! ACTGAAAATATTTGAGATGA+TGG + chr7_4:62666441-62666460 Msa1169640:three_prime_UTR 25.0%
TATTGCTATAAGGTAAGAAG+AGG + chr7_4:62663280-62663299 Msa1169640:five_prime_UTR 30.0%
ATATTCACGAGAACAATGAA+GGG + chr7_4:62663335-62663354 Msa1169640:five_prime_UTR 30.0%
TTCAGTTATGAAGATTTGGA+GGG - chr7_4:62663434-62663453 None:intergenic 30.0%
ATTCAGTTATGAAGATTTGG+AGG - chr7_4:62663435-62663454 None:intergenic 30.0%
ACGATTCAGTTATGAAGATT+TGG - chr7_4:62663438-62663457 None:intergenic 30.0%
!! TCTGTTTTCGTTTTTCTGTA+GGG + chr7_4:62664038-62664057 Msa1169640:intron 30.0%
TGTGGAAAACAAGTATACTT+AGG + chr7_4:62664063-62664082 Msa1169640:CDS 30.0%
TGCTATAAAATGCAATGGAA+GGG + chr7_4:62665090-62665109 Msa1169640:CDS 30.0%
AGAGTGAGTGATAGTTTATA+TGG - chr7_4:62665256-62665275 None:intergenic 30.0%
ATCACCAGTCTTAATAGTAA+AGG - chr7_4:62665313-62665332 None:intergenic 30.0%
ATTTGAACATCAGAGTTAGA+AGG - chr7_4:62665423-62665442 None:intergenic 30.0%
! TTGATAAGAGTGTAATGGTA+AGG + chr7_4:62665505-62665524 Msa1169640:intron 30.0%
ATTAGTATTCACCTTACACA+CGG - chr7_4:62665754-62665773 None:intergenic 30.0%
ATATCTTAAGAATCTTCTGC+TGG + chr7_4:62665833-62665852 Msa1169640:intron 30.0%
CTCCAAAGTCCATATAATAA+TGG + chr7_4:62665947-62665966 Msa1169640:CDS 30.0%
ACCCATTATTATATGGACTT+TGG - chr7_4:62665952-62665971 None:intergenic 30.0%
!! GAATTGTAGCTTTATACTCT+AGG - chr7_4:62666154-62666173 None:intergenic 30.0%
ATGATCATATTCCTAAACCT+TGG - chr7_4:62666207-62666226 None:intergenic 30.0%
ATGATAACAAAGGAAGGAAA+TGG - chr7_4:62666399-62666418 None:intergenic 30.0%
GTGAAAATGATAACAAAGGA+AGG - chr7_4:62666405-62666424 None:intergenic 30.0%
GAGAGTGAAAATGATAACAA+AGG - chr7_4:62666409-62666428 None:intergenic 30.0%
! AATATTTGAGATGATGGTGA+AGG + chr7_4:62666447-62666466 Msa1169640:three_prime_UTR 30.0%
CATATTCACGAGAACAATGA+AGG + chr7_4:62663334-62663353 Msa1169640:five_prime_UTR 35.0%
!!! GAAGGGAAGCATTTTCATTT+TGG + chr7_4:62663352-62663371 Msa1169640:five_prime_UTR 35.0%
GAATGAAAGATAGAGAGAGA+AGG - chr7_4:62663380-62663399 None:intergenic 35.0%
AAACTCAAACCTCACAATGT+TGG + chr7_4:62663461-62663480 Msa1169640:exon 35.0%
ACTGAAGATTCATGCTCTTA+CGG + chr7_4:62663585-62663604 Msa1169640:CDS 35.0%
ATTTGGTTAGCATTTCGCAA+TGG + chr7_4:62663942-62663961 Msa1169640:intron 35.0%
ACAGAAAAACGAAAACAGAG+TGG - chr7_4:62664037-62664056 None:intergenic 35.0%
!! CTCTGTTTTCGTTTTTCTGT+AGG + chr7_4:62664037-62664056 Msa1169640:intron 35.0%
! ATATTGGTCTGGGTTTATGT+AGG + chr7_4:62664873-62664892 Msa1169640:intron 35.0%
! TACGTGTTGTGTTATTGACT+TGG + chr7_4:62665031-62665050 Msa1169640:intron 35.0%
TGATTACAGAGCGTATGATA+AGG + chr7_4:62665066-62665085 Msa1169640:intron 35.0%
AAGGCTGCTATAAAATGCAA+TGG + chr7_4:62665085-62665104 Msa1169640:CDS 35.0%
CTGCTATAAAATGCAATGGA+AGG + chr7_4:62665089-62665108 Msa1169640:CDS 35.0%
! GGAAGCAGTTACAAATTTTG+AGG + chr7_4:62665111-62665130 Msa1169640:CDS 35.0%
! TTTGAGGCTAGTTCTTATGA+AGG + chr7_4:62665127-62665146 Msa1169640:CDS 35.0%
GTACTACTTATTGACCATTG+AGG + chr7_4:62665179-62665198 Msa1169640:intron 35.0%
AAAAAAAAGGTGAGCCTCAA+TGG - chr7_4:62665196-62665215 None:intergenic 35.0%
!!! ATTGAGGCTCACCTTTTTTT+TGG + chr7_4:62665195-62665214 Msa1169640:intron 35.0%
CAGAATCTTGATCTGAACTT+GGG + chr7_4:62665387-62665406 Msa1169640:CDS 35.0%
ACCATTACACTCTTATCAAC+AGG - chr7_4:62665504-62665523 None:intergenic 35.0%
AATTCATGTGAAAGAGCAGA+GGG - chr7_4:62665577-62665596 None:intergenic 35.0%
!! TAATCTGCGTTGATAGTTTG+AGG + chr7_4:62665632-62665651 Msa1169640:intron 35.0%
CTCAATGTTCAGTCATCTTA+TGG + chr7_4:62665672-62665691 Msa1169640:CDS 35.0%
TAATTAGTACCACTCCAGAT+TGG - chr7_4:62665726-62665745 None:intergenic 35.0%
TAATTACTTTCCCGTGTGTA+AGG + chr7_4:62665740-62665759 Msa1169640:CDS 35.0%
TTAGTATTCACCTTACACAC+GGG - chr7_4:62665753-62665772 None:intergenic 35.0%
AGGAAAGAGCAATAGAGAAT+AGG + chr7_4:62665888-62665907 Msa1169640:intron 35.0%
AAGAGCAATAGAGAATAGGA+TGG + chr7_4:62665892-62665911 Msa1169640:CDS 35.0%
AGTCTTTGTATCCAAGGTTT+AGG + chr7_4:62666193-62666212 Msa1169640:three_prime_UTR 35.0%
AACTTGTAGGGTCTAAAAGT+AGG + chr7_4:62666229-62666248 Msa1169640:three_prime_UTR 35.0%
GGTAAGAAGAGGAATAGAAG+AGG + chr7_4:62663291-62663310 Msa1169640:five_prime_UTR 40.0%
GATTGAGATCCAACATTGTG+AGG - chr7_4:62663473-62663492 None:intergenic 40.0%
TGAATCTTCCATCTGAACGA+CGG - chr7_4:62663526-62663545 None:intergenic 40.0%
GTTCAGATGGAAGATTCATC+CGG + chr7_4:62663528-62663547 Msa1169640:CDS 40.0%
AGAGCATGAATCTTCAGTGT+CGG - chr7_4:62663583-62663602 None:intergenic 40.0%
!! GCTTTTTCCAGCAAACGAAT+CGG + chr7_4:62663725-62663744 Msa1169640:CDS 40.0%
! CTTTTTCCAGCAAACGAATC+GGG + chr7_4:62663726-62663745 Msa1169640:CDS 40.0%
AATTTCTCGTCCGATCATCA+CGG + chr7_4:62663783-62663802 Msa1169640:CDS 40.0%
!! ATCGTGGTGTTACTTTCTAC+CGG + chr7_4:62663895-62663914 Msa1169640:CDS 40.0%
!! TCGTTTTTCTGTAGGGATTG+TGG + chr7_4:62664045-62664064 Msa1169640:intron 40.0%
!! ACAGAGTACTGGTTTCTCAA+GGG + chr7_4:62664652-62664671 Msa1169640:CDS 40.0%
!! AGGGGAAGTTCTAAATACAG+AGG + chr7_4:62664671-62664690 Msa1169640:CDS 40.0%
GGAGTTACTCTGCATAAATG+TGG + chr7_4:62664692-62664711 Msa1169640:CDS 40.0%
TCTGCATAAATGTGGACGAT+GGG + chr7_4:62664700-62664719 Msa1169640:CDS 40.0%
GCAGAATCTTGATCTGAACT+TGG + chr7_4:62665386-62665405 Msa1169640:CDS 40.0%
TGAACATCAGAGTTAGAAGG+AGG - chr7_4:62665420-62665439 None:intergenic 40.0%
TTTGCAAGTTCAGCGTAACT+GGG + chr7_4:62665470-62665489 Msa1169640:CDS 40.0%
! GCCTGTTGATAAGAGTGTAA+TGG + chr7_4:62665500-62665519 Msa1169640:CDS 40.0%
ATTCATGTGAAAGAGCAGAG+GGG - chr7_4:62665576-62665595 None:intergenic 40.0%
GAATTCATGTGAAAGAGCAG+AGG - chr7_4:62665578-62665597 None:intergenic 40.0%
!! GTTGATAGTTTGAGGACTCT+GGG + chr7_4:62665640-62665659 Msa1169640:intron 40.0%
! ACGTCTAGTCTTTGTATCCA+AGG + chr7_4:62666187-62666206 Msa1169640:three_prime_UTR 40.0%
!!! CGGCAGGGATTTTTTTTACA+CGG - chr7_4:62666319-62666338 None:intergenic 40.0%
TAAAAAAAATCCCTGCCGCA+AGG + chr7_4:62666321-62666340 Msa1169640:three_prime_UTR 40.0%
!! GGAAGCGTTGTTGTTGAGTA+TGG - chr7_4:62663634-62663653 None:intergenic 45.0%
ATCCATCCTCTACTCTCAGA+AGG - chr7_4:62663762-62663781 None:intergenic 45.0%
! GTTACTTTCTACCGGAGAAC+TGG + chr7_4:62663903-62663922 Msa1169640:CDS 45.0%
GACGTTGGGAATCGCATATT+TGG + chr7_4:62663925-62663944 Msa1169640:CDS 45.0%
ATTCTGCGTAGACAGAGTAC+TGG + chr7_4:62664641-62664660 Msa1169640:CDS 45.0%
!! GACAGAGTACTGGTTTCTCA+AGG + chr7_4:62664651-62664670 Msa1169640:CDS 45.0%
!! CAGAGTACTGGTTTCTCAAG+GGG + chr7_4:62664653-62664672 Msa1169640:CDS 45.0%
CTCTGCATAAATGTGGACGA+TGG + chr7_4:62664699-62664718 Msa1169640:CDS 45.0%
CTCGTATGGGACAGTTTCTT+GGG + chr7_4:62664726-62664745 Msa1169640:CDS 45.0%
TGAAGGAGAGTTAACTTCCC+AGG + chr7_4:62665144-62665163 Msa1169640:CDS 45.0%
AGTACCATCGTTATCAGCCT+GGG - chr7_4:62665164-62665183 None:intergenic 45.0%
TAGTACCATCGTTATCAGCC+TGG - chr7_4:62665165-62665184 None:intergenic 45.0%
ATGATGAACATGCACCACAG+TGG + chr7_4:62665441-62665460 Msa1169640:CDS 45.0%
GTTTGCAAGTTCAGCGTAAC+TGG + chr7_4:62665469-62665488 Msa1169640:CDS 45.0%
!! CGTTGATAGTTTGAGGACTC+TGG + chr7_4:62665639-62665658 Msa1169640:intron 45.0%
! GGTTGATGCTCAGATGCTAT+GGG - chr7_4:62665702-62665721 None:intergenic 45.0%
!! AGGTTGATGCTCAGATGCTA+TGG - chr7_4:62665703-62665722 None:intergenic 45.0%
TTAGTACCACTCCAGATTGG+AGG - chr7_4:62665723-62665742 None:intergenic 45.0%
AATCTGCTCCCGTTCCAAAT+TGG + chr7_4:62665915-62665934 Msa1169640:CDS 45.0%
ATCTGCTCCCGTTCCAAATT+GGG + chr7_4:62665916-62665935 Msa1169640:CDS 45.0%
GATGCTGCAGCAGAGAAATT+AGG - chr7_4:62665989-62666008 None:intergenic 45.0%
ATTCCCTTCAACAACAGCTG+CGG + chr7_4:62666015-62666034 Msa1169640:CDS 45.0%
TATCCGCAGCTGTTGTTGAA+GGG - chr7_4:62666021-62666040 None:intergenic 45.0%
! GGGTGGAGGTATGTTATGTT+TGG - chr7_4:62666058-62666077 None:intergenic 45.0%
TCGCTGGAGTAGGAAAATGA+GGG - chr7_4:62666078-62666097 None:intergenic 45.0%
! TGCAGTAGAAAGAGGGTATG+GGG - chr7_4:62666111-62666130 None:intergenic 45.0%
! CTGCAGTAGAAAGAGGGTAT+GGG - chr7_4:62666112-62666131 None:intergenic 45.0%
CTAGAGTTTACCATGCCTTG+CGG - chr7_4:62666339-62666358 None:intergenic 45.0%
ATCTTCCATCTGAACGACGG+AGG - chr7_4:62663523-62663542 None:intergenic 50.0%
GACGGAGGAATCAAAGCTAC+CGG - chr7_4:62663550-62663569 None:intergenic 50.0%
TGCTCTTACGGCGATGCATA+CGG + chr7_4:62663597-62663616 Msa1169640:CDS 50.0%
AACAACGCTTCCGTCGACAA+CGG + chr7_4:62663642-62663661 Msa1169640:CDS 50.0%
GGAGATAGTACCGTTGTCGA+CGG - chr7_4:62663655-62663674 None:intergenic 50.0%
GTCGACAACGGTACTATCTC+CGG + chr7_4:62663654-62663673 Msa1169640:CDS 50.0%
! GTCATTCCCGATTCGTTTGC+TGG - chr7_4:62663735-62663754 None:intergenic 50.0%
GCAACAACAACAGTCTGCTC+CGG - chr7_4:62663816-62663835 None:intergenic 50.0%
ATTCCCAACGTCCAGTTCTC+CGG - chr7_4:62663917-62663936 None:intergenic 50.0%
ATTTGATACCGCGCATGCTG+CGG + chr7_4:62664203-62664222 Msa1169640:CDS 50.0%
CGAGCTGCGATAAAGTTTCG+TGG + chr7_4:62664342-62664361 Msa1169640:CDS 50.0%
!! AAAGTTTCGTGGCGTCGATG+CGG + chr7_4:62664353-62664372 Msa1169640:CDS 50.0%
GCTCGTATGGGACAGTTTCT+TGG + chr7_4:62664725-62664744 Msa1169640:CDS 50.0%
ATAGCGAGCTAGATGCTGCA+AGG + chr7_4:62664918-62664937 Msa1169640:CDS 50.0%
! ACTTCCCAGGCTGATAACGA+TGG + chr7_4:62665157-62665176 Msa1169640:CDS 50.0%
AACATGCACCACAGTGGAAG+TGG + chr7_4:62665447-62665466 Msa1169640:CDS 50.0%
CTTGCAAACCACTTCCACTG+TGG - chr7_4:62665458-62665477 None:intergenic 50.0%
CTGAGCATCAACCTCCAATC+TGG + chr7_4:62665709-62665728 Msa1169640:CDS 50.0%
CATCAACCTCCAATCTGGAG+TGG + chr7_4:62665714-62665733 Msa1169640:CDS 50.0%
! TGCCATGCCCAATTTGGAAC+GGG - chr7_4:62665926-62665945 None:intergenic 50.0%
CTGCCATGCCCAATTTGGAA+CGG - chr7_4:62665927-62665946 None:intergenic 50.0%
GCTGCAGCAGAGAAATTAGG+TGG - chr7_4:62665986-62666005 None:intergenic 50.0%
TTCTCTGCTGCAGCATCATC+AGG + chr7_4:62665992-62666011 Msa1169640:CDS 50.0%
CTATCCGCAGCTGTTGTTGA+AGG - chr7_4:62666022-62666041 None:intergenic 50.0%
GAGTAGGAAAATGAGGGTGG+AGG - chr7_4:62666072-62666091 None:intergenic 50.0%
CTGGAGTAGGAAAATGAGGG+TGG - chr7_4:62666075-62666094 None:intergenic 50.0%
GTCGCTGGAGTAGGAAAATG+AGG - chr7_4:62666079-62666098 None:intergenic 50.0%
CCTGCAGTAGAAAGAGGGTA+TGG - chr7_4:62666113-62666132 None:intergenic 50.0%
CCATACCCTCTTTCTACTGC+AGG + chr7_4:62666110-62666129 Msa1169640:CDS 50.0%
CAGCTCCTGCAGTAGAAAGA+GGG - chr7_4:62666118-62666137 None:intergenic 50.0%
TCAGCTCCTGCAGTAGAAAG+AGG - chr7_4:62666119-62666138 None:intergenic 50.0%
ATCTGAACGACGGAGGAGAG+TGG - chr7_4:62663516-62663535 None:intergenic 55.0%
ACTCTCCTCCGTCGTTCAGA+TGG + chr7_4:62663515-62663534 Msa1169640:CDS 55.0%
TCCGGTGAGCTTCAACACTC+CGG + chr7_4:62663672-62663691 Msa1169640:CDS 55.0%
TGTCCGTACAACAGCTTCGC+CGG - chr7_4:62663700-62663719 None:intergenic 55.0%
! ACCTCGCCTTCTGAGAGTAG+AGG + chr7_4:62663753-62663772 Msa1169640:CDS 55.0%
TCCTCTACTCTCAGAAGGCG+AGG - chr7_4:62663757-62663776 None:intergenic 55.0%
! CGCCTTCTGAGAGTAGAGGA+TGG + chr7_4:62663757-62663776 Msa1169640:CDS 55.0%
TTCTCGTCCGATCATCACGG+TGG + chr7_4:62663786-62663805 Msa1169640:CDS 55.0%
TTCTTCACCTGCTGAGGTCG+TGG - chr7_4:62663840-62663859 None:intergenic 55.0%
CGACTCTTCTTCACCTGCTG+AGG - chr7_4:62663846-62663865 None:intergenic 55.0%
CAGGTGAAGAAGAGTCGTCG+TGG + chr7_4:62663849-62663868 Msa1169640:CDS 55.0%
TACTGCGAGCTTCGTGACCT+CGG - chr7_4:62663876-62663895 None:intergenic 55.0%
TCACGAAGCTCGCAGTATCG+TGG + chr7_4:62663879-62663898 Msa1169640:CDS 55.0%
TCTACCGGAGAACTGGACGT+TGG + chr7_4:62663910-62663929 Msa1169640:CDS 55.0%
CTACCGGAGAACTGGACGTT+GGG + chr7_4:62663911-62663930 Msa1169640:CDS 55.0%
TGGACGATGGGAAGCTCGTA+TGG + chr7_4:62664712-62664731 Msa1169640:CDS 55.0%
GGACGATGGGAAGCTCGTAT+GGG + chr7_4:62664713-62664732 Msa1169640:CDS 55.0%
! TGAAAGAGCAGAGGGGCATG+AGG - chr7_4:62665569-62665588 None:intergenic 55.0%
CTCCCGTTCCAAATTGGGCA+TGG + chr7_4:62665921-62665940 Msa1169640:CDS 55.0%
TGGAGCTGCCATGCCCAATT+TGG - chr7_4:62665932-62665951 None:intergenic 55.0%
CTGGAGTTAGTCGCTGGAGT+AGG - chr7_4:62666088-62666107 None:intergenic 55.0%
! GTAGAAAGAGGGTATGGGGC+TGG - chr7_4:62666107-62666126 None:intergenic 55.0%
AAAATCCCTGCCGCAAGGCA+TGG + chr7_4:62666326-62666345 Msa1169640:three_prime_UTR 55.0%
GTTTACCATGCCTTGCGGCA+GGG - chr7_4:62666334-62666353 None:intergenic 55.0%
AGTTTACCATGCCTTGCGGC+AGG - chr7_4:62666335-62666354 None:intergenic 55.0%
GACGGAGGAGAGTGGAAGAG+AGG - chr7_4:62663508-62663527 None:intergenic 60.0%
!! AGTGTCGGCAGCGTTGACGA+CGG - chr7_4:62663568-62663587 None:intergenic 60.0%
!! GCCGGAGTGTTGAAGCTCAC+CGG - chr7_4:62663676-62663695 None:intergenic 60.0%
CGGTACGCCACCGTGATGAT+CGG - chr7_4:62663796-62663815 None:intergenic 60.0%
CGATCATCACGGTGGCGTAC+CGG + chr7_4:62663794-62663813 Msa1169640:CDS 60.0%
TGTTGCTCCACGACCTCAGC+AGG + chr7_4:62663830-62663849 Msa1169640:CDS 60.0%
AGAAGAGTCGTCGTGGACCG+AGG + chr7_4:62663856-62663875 Msa1169640:CDS 60.0%
TTACCTTGCCGCAGCATGCG+CGG - chr7_4:62664214-62664233 None:intergenic 60.0%
ATGGGGCTGGAGTTAGTCGC+TGG - chr7_4:62666094-62666113 None:intergenic 60.0%
GAGCTTCAACACTCCGGCGC+CGG + chr7_4:62663678-62663697 Msa1169640:CDS 65.0%
TACAACAGCTTCGCCGGCGC+CGG - chr7_4:62663694-62663713 None:intergenic 65.0%
! GCGCCGGCGAAGCTGTTGTA+CGG + chr7_4:62663694-62663713 Msa1169640:CDS 65.0%
ATACCGCGCATGCTGCGGCA+AGG + chr7_4:62664208-62664227 Msa1169640:CDS 65.0%
!! GTCGGCAGCGTTGACGACGG+AGG - chr7_4:62663565-62663584 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 62663266 62666543 62663266 ID=Msa1169640;Name=Msa1169640
chr7_4 mRNA 62663266 62666543 62663266 ID=Msa1169640-mRNA-1;Parent=Msa1169640;Name=Msa1169640-mRNA-1;_AED=0.14;_eAED=0.14;_QI=211|1|1|1|1|1|10|405|471
chr7_4 exon 62663266 62663946 62663266 ID=Msa1169640-mRNA-1:exon:19866;Parent=Msa1169640-mRNA-1
chr7_4 exon 62664059 62664084 62664059 ID=Msa1169640-mRNA-1:exon:19867;Parent=Msa1169640-mRNA-1
chr7_4 exon 62664199 62664229 62664199 ID=Msa1169640-mRNA-1:exon:19868;Parent=Msa1169640-mRNA-1
chr7_4 exon 62664332 62664419 62664332 ID=Msa1169640-mRNA-1:exon:19869;Parent=Msa1169640-mRNA-1
chr7_4 exon 62664608 62664753 62664608 ID=Msa1169640-mRNA-1:exon:19870;Parent=Msa1169640-mRNA-1
chr7_4 exon 62664895 62664939 62664895 ID=Msa1169640-mRNA-1:exon:19871;Parent=Msa1169640-mRNA-1
chr7_4 exon 62665075 62665178 62665075 ID=Msa1169640-mRNA-1:exon:19872;Parent=Msa1169640-mRNA-1
chr7_4 exon 62665379 62665521 62665379 ID=Msa1169640-mRNA-1:exon:19873;Parent=Msa1169640-mRNA-1
chr7_4 exon 62665648 62665761 62665648 ID=Msa1169640-mRNA-1:exon:19874;Parent=Msa1169640-mRNA-1
chr7_4 exon 62665890 62666543 62665890 ID=Msa1169640-mRNA-1:exon:19875;Parent=Msa1169640-mRNA-1
chr7_4 five_prime_UTR 62663266 62663476 62663266 ID=Msa1169640-mRNA-1:five_prime_utr;Parent=Msa1169640-mRNA-1
chr7_4 CDS 62663477 62663946 62663477 ID=Msa1169640-mRNA-1:cds;Parent=Msa1169640-mRNA-1
chr7_4 CDS 62664059 62664084 62664059 ID=Msa1169640-mRNA-1:cds;Parent=Msa1169640-mRNA-1
chr7_4 CDS 62664199 62664229 62664199 ID=Msa1169640-mRNA-1:cds;Parent=Msa1169640-mRNA-1
chr7_4 CDS 62664332 62664419 62664332 ID=Msa1169640-mRNA-1:cds;Parent=Msa1169640-mRNA-1
chr7_4 CDS 62664608 62664753 62664608 ID=Msa1169640-mRNA-1:cds;Parent=Msa1169640-mRNA-1
chr7_4 CDS 62664895 62664939 62664895 ID=Msa1169640-mRNA-1:cds;Parent=Msa1169640-mRNA-1
chr7_4 CDS 62665075 62665178 62665075 ID=Msa1169640-mRNA-1:cds;Parent=Msa1169640-mRNA-1
chr7_4 CDS 62665379 62665521 62665379 ID=Msa1169640-mRNA-1:cds;Parent=Msa1169640-mRNA-1
chr7_4 CDS 62665648 62665761 62665648 ID=Msa1169640-mRNA-1:cds;Parent=Msa1169640-mRNA-1
chr7_4 CDS 62665890 62666138 62665890 ID=Msa1169640-mRNA-1:cds;Parent=Msa1169640-mRNA-1
chr7_4 three_prime_UTR 62666139 62666543 62666139 ID=Msa1169640-mRNA-1:three_prime_utr;Parent=Msa1169640-mRNA-1
Gene Sequence

>Msa1169640

ATGTTGGATCTCAATCTCAACGCTGATTCCTCTCTTCCACTCTCCTCCGTCGTTCAGATGGAAGATTCATCCGGTAGCTTTGATTCCTCCGTCGTCAACGCTGCCGACACTGAAGATTCATGCTCTTACGGCGATGCATACGGTTACAACTTCGCCATACTCAACAACAACGCTTCCGTCGACAACGGTACTATCTCCGGTGAGCTTCAACACTCCGGCGCCGGCGAAGCTGTTGTACGGACAATTCAGCTTTTTCCAGCAAACGAATCGGGAATGACCTCGCCTTCTGAGAGTAGAGGATGGATGAATTTCTCGTCCGATCATCACGGTGGCGTACCGGAGCAGACTGTTGTTGTTGCTCCACGACCTCAGCAGGTGAAGAAGAGTCGTCGTGGACCGAGGTCACGAAGCTCGCAGTATCGTGGTGTTACTTTCTACCGGAGAACTGGACGTTGGGAATCGCATATTTGGGATTGTGGAAAACAAGTATACTTAGGTGGATTTGATACCGCGCATGCTGCGGCAAGAGCATATGATCGAGCTGCGATAAAGTTTCGTGGCGTCGATGCGGATATTAATTTCAATGTCAGTGATTATGATGAAGATATAAAGCAGATGAATAACTTTACAAAGGAAGAGTTTGTGCATATTCTGCGTAGACAGAGTACTGGTTTCTCAAGGGGAAGTTCTAAATACAGAGGAGTTACTCTGCATAAATGTGGACGATGGGAAGCTCGTATGGGACAGTTTCTTGGGAAGAAGTATATATATCTTGGGCTTTTTGATAGCGAGCTAGATGCTGCAAGAGCGTATGATAAGGCTGCTATAAAATGCAATGGAAGGGAAGCAGTTACAAATTTTGAGGCTAGTTCTTATGAAGGAGAGTTAACTTCCCAGGCTGATAACGATGATATCAAGCAGAATCTTGATCTGAACTTGGGTATAGCTCCTCCTTCTAACTCTGATGTTCAAATGATGAACATGCACCACAGTGGAAGTGGTTTGCAAGTTCAGCGTAACTGGGATGATATGCCTGTTGATAAGAGTGTAATGTTTGAGGACTCTGGGTCAAGATCACTCAATGTTCAGTCATCTTATGGCTTTCCCATAGCATCTGAGCATCAACCTCCAATCTGGAGTGGTACTAATTACTTTCCCGTGTGTAAGGAAAGAGCAATAGAGAATAGGATGGAATCTGCTCCCGTTCCAAATTGGGCATGGCAGCTCCAAAGTCCATATAATAATGGGTCAACTTTGATTCCACCTAATTTCTCTGCTGCAGCATCATCAGGATTCCCTTCAACAACAGCTGCGGATAGTCAGATTCATTTTCCAAACATAACATACCTCCACCCTCATTTTCCTACTCCAGCGACTAACTCCAGCCCCATACCCTCTTTCTACTGCAGGAGCTGA
Protein sequence

>Msa1169640

MLDLNLNADSSLPLSSVVQMEDSSGSFDSSVVNAADTEDSCSYGDAYGYNFAILNNNASVDNGTISGELQHSGAGEAVVRTIQLFPANESGMTSPSESRGWMNFSSDHHGGVPEQTVVVAPRPQQVKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAHAAARAYDRAAIKFRGVDADINFNVSDYDEDIKQMNNFTKEEFVHILRRQSTGFSRGSSKYRGVTLHKCGRWEARMGQFLGKKYIYLGLFDSELDAARAYDKAAIKCNGREAVTNFEASSYEGELTSQADNDDIKQNLDLNLGIAPPSNSDVQMMNMHHSGSGLQVQRNWDDMPVDKSVMFEDSGSRSLNVQSSYGFPIASEHQPPIWSGTNYFPVCKERAIENRMESAPVPNWAWQLQSPYNNGSTLIPPNFSAAASSGFPSTTAADSQIHFPNITYLHPHFPTPATNSSPIPSFYCRS*