AlfalfaGEDB | Msa1185320

M. sativa cultivar zhongmu-4 / Msa1185320

KEGG: K09260
GO: GO:0000003 GO:0000060 GO:0001101 GO:0003006 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006606 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0007275 GO:0008104 GO:0008134 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009739 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009909 GO:0009911 GO:0010033 GO:0010073 GO:0010074 GO:0010077 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015031 GO:0015833 GO:0017038 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0033993 GO:0034504 GO:0034613 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045184 GO:0045893 GO:0045935 GO:0046907 GO:0048367 GO:0048507 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0060255 GO:0061458 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0080090 GO:0090567 GO:0098727 GO:0140110 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1185320	sp\|O64645\|SOC1_ARATH	65.438	217	56	4	1	206	1	209	1.68e-89	265

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1185320	A0A072TND6	91.518	224	12	1	1	217	1	224	2.11e-141	403

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TFs/TRs:

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Gene ID	Type	Classification
Msa1185320	TF	MADS-MIKC

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0306810	Msa1185320	0.893375	6.667147e-75	-8.615850e-47
Msa1037620	Msa1185320	0.823199	1.575421e-53	-8.615850e-47
Msa1130300	Msa1185320	0.823360	1.444588e-53	-8.615850e-47
Msa1185290	Msa1185320	0.886974	2.143120e-72	-8.615850e-47
Msa1185310	Msa1185320	0.871875	4.895935e-67	-8.615850e-47
Msa1185320	Msa1239320	0.909357	6.229224e-82	-8.615850e-47
Msa1185320	Msa1329230	0.818536	1.862737e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1185320	MtrunA17_Chr8g0351031	91.518	224	12	1	1	217	1	224	4.06e-145	403
Msa1185320	MtrunA17_Chr8g0351041	91.925	161	13	0	1	161	1	161	1.04e-105	301
Msa1185320	MtrunA17_Chr7g0246431	66.981	212	59	4	1	205	1	208	3.59e-94	274
Msa1185320	MtrunA17_Chr4g0057151	58.333	216	70	4	1	209	1	203	1.34e-76	229
Msa1185320	MtrunA17_Chr3g0097101	45.455	209	105	3	1	203	16	221	3.93e-53	171
Msa1185320	MtrunA17_Chr2g0284911	49.714	175	87	1	3	177	18	191	3.68e-52	169
Msa1185320	MtrunA17_Chr8g0351051	42.922	219	108	3	1	203	1	218	1.86e-49	161
Msa1185320	MtrunA17_Chr3g0077501	47.953	171	88	1	1	171	1	170	6.52e-49	159
Msa1185320	MtrunA17_Chr8g0380021	46.067	178	95	1	3	180	19	195	5.47e-48	157
Msa1185320	MtrunA17_Chr8g0366231	50.296	169	82	2	1	168	1	168	8.39e-47	154

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1185320	AT2G45660.1	65.438	217	56	4	1	206	1	209	1.70e-90	265
Msa1185320	AT2G45660.2	75.155	161	35	2	1	159	1	158	1.88e-82	243
Msa1185320	AT5G62165.3	56.808	213	78	3	1	206	1	206	1.69e-79	237
Msa1185320	AT5G62165.1	56.808	213	78	3	1	206	1	206	1.69e-79	237
Msa1185320	AT5G62165.2	56.808	213	78	3	1	206	1	206	1.69e-79	237
Msa1185320	AT4G22950.3	58.447	219	84	3	1	215	1	216	3.73e-76	229
Msa1185320	AT4G22950.1	58.447	219	84	3	1	215	1	216	3.73e-76	229
Msa1185320	AT4G22950.2	65.497	171	57	1	1	171	1	169	2.84e-74	222
Msa1185320	AT5G62165.17	63.694	157	56	1	1	157	1	156	6.94e-71	213
Msa1185320	AT5G62165.12	63.694	157	56	1	1	157	1	156	6.94e-71	213

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Find 70 sgRNAs with CRISPR-Local

Find 670 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCATAATTGCTTGTTT+TGG	0.291781	8_1:+22376386	None:intergenic
AAACTCTTAGGAGAAGGTCT+AGG	0.447821	8_1:-22366469	Msa1185320:CDS
AAAGAAAATATATGTATAAA+GGG	0.409393	8_1:+22376082	None:intergenic
AAAGTAGTGATCCATAATTG+AGG	0.623208	8_1:+22376195	None:intergenic
AAATGCCATGCTCACAGAGA+AGG	0.595558	8_1:-22365699	Msa1185320:intron
AACAAGACGCATTTCTCCAA+AGG	0.605530	8_1:-22365249	Msa1185320:CDS
AACGATACCGCAATCATACC+AGG	0.576369	8_1:-22369623	Msa1185320:CDS
AACTCTTAGGAGAAGGTCTA+GGG	0.544433	8_1:-22366468	Msa1185320:CDS
AAGCAAAATTCGAGCAAGAA+AGG	0.486364	8_1:-22366392	Msa1185320:intron
AAGTAGTCCAAGTTCAGATG+TGG	0.602735	8_1:-22365306	Msa1185320:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTGATTAATAAGGAAAGGT+AGG	-	chr8_1:22365055-22365074	Msa1185320:three_prime_UTR	30.0%
AAAGTAGTGATCCATAATTG+AGG	+	chr8_1:22365153-22365172	None:intergenic	30.0%
TAGAACAGGAAAATAAGGAA+GGG	+	chr8_1:22365236-22365255	None:intergenic	30.0%
GCAAGAAAAGCAATTTCAAA+AGG	+	chr8_1:22365318-22365337	None:intergenic	30.0%
GATGAAAGAAACAAGGAAAT+GGG	+	chr8_1:22365477-22365496	None:intergenic	30.0%
TATGAAGGATGAAAGAAACA+AGG	+	chr8_1:22365484-22365503	None:intergenic	30.0%
ACATTCTCAAAGAGAAGAAA+TGG	-	chr8_1:22365875-22365894	Msa1185320:intron	30.0%
ACACAACACTGATAATTCAA+AGG	+	chr8_1:22365915-22365934	None:intergenic	30.0%
CTAGAGTAAACAAACTCTAA+GGG	+	chr8_1:22366347-22366366	None:intergenic	30.0%
AGAAGTGAGAGAATGAAAAT+AGG	-	chr8_1:22366398-22366417	Msa1185320:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	22364953	22376414	22364953	ID=Msa1185320;Name=Msa1185320
chr8_1	mRNA	22364953	22376414	22364953	ID=Msa1185320-mRNA-1;Parent=Msa1185320;Name=Msa1185320-mRNA-1;_AED=0.14;_eAED=0.14;_QI=409\|1\|1\|1\|0.85\|0.75\|8\|291\|217
chr8_1	exon	22364953	22365384	22364953	ID=Msa1185320-mRNA-1:exon:8256;Parent=Msa1185320-mRNA-1
chr8_1	exon	22365700	22365741	22365700	ID=Msa1185320-mRNA-1:exon:8255;Parent=Msa1185320-mRNA-1
chr8_1	exon	22366273	22366314	22366273	ID=Msa1185320-mRNA-1:exon:8254;Parent=Msa1185320-mRNA-1
chr8_1	exon	22366393	22366492	22366393	ID=Msa1185320-mRNA-1:exon:8253;Parent=Msa1185320-mRNA-1
chr8_1	exon	22366605	22366666	22366605	ID=Msa1185320-mRNA-1:exon:8252;Parent=Msa1185320-mRNA-1
chr8_1	exon	22369578	22369662	22369578	ID=Msa1185320-mRNA-1:exon:8251;Parent=Msa1185320-mRNA-1
chr8_1	exon	22375368	22375566	22375368	ID=Msa1185320-mRNA-1:exon:8250;Parent=Msa1185320-mRNA-1
chr8_1	exon	22376023	22376414	22376023	ID=Msa1185320-mRNA-1:exon:8249;Parent=Msa1185320-mRNA-1
chr8_1	five_prime_UTR	22376023	22376414	22376023	ID=Msa1185320-mRNA-1:five_prime_utr;Parent=Msa1185320-mRNA-1
chr8_1	five_prime_UTR	22375550	22375566	22375550	ID=Msa1185320-mRNA-1:five_prime_utr;Parent=Msa1185320-mRNA-1
chr8_1	CDS	22375368	22375549	22375368	ID=Msa1185320-mRNA-1:cds;Parent=Msa1185320-mRNA-1
chr8_1	CDS	22369578	22369662	22369578	ID=Msa1185320-mRNA-1:cds;Parent=Msa1185320-mRNA-1
chr8_1	CDS	22366605	22366666	22366605	ID=Msa1185320-mRNA-1:cds;Parent=Msa1185320-mRNA-1
chr8_1	CDS	22366393	22366492	22366393	ID=Msa1185320-mRNA-1:cds;Parent=Msa1185320-mRNA-1
chr8_1	CDS	22366273	22366314	22366273	ID=Msa1185320-mRNA-1:cds;Parent=Msa1185320-mRNA-1
chr8_1	CDS	22365700	22365741	22365700	ID=Msa1185320-mRNA-1:cds;Parent=Msa1185320-mRNA-1
chr8_1	CDS	22365244	22365384	22365244	ID=Msa1185320-mRNA-1:cds;Parent=Msa1185320-mRNA-1
chr8_1	three_prime_UTR	22364953	22365243	22364953	ID=Msa1185320-mRNA-1:three_prime_utr;Parent=Msa1185320-mRNA-1

Gene Sequence

>Msa1185320

ATGGTGAGAGGAAAGACACAAATGAAGCGTATAGAGAACGCAACAAGTAGGCAAGTAACATTCTCAAAGAGAAGAAATGGTTTGTTGAAGAAAGCCTTTGAATTATCAGTGTTGTGTGATGCTGAGGTTGCTCTTATTGTTTTCTCACCAAGAGGGAGGCTATATGAATTTGCTAGCTCTAGCATTCCAGAAACGATTGAACGATACCGCAATCATACCAGGATTAATAGCACTCCAACACCATCTGAATCAGTTGAAAATACTCAGCAATTGAAGGAAGAAGCAGAAAACATGATGAAAAAGATTGACCTTCTTGAGACTTCAAAACGGAAACTCTTAGGAGAAGGTCTAGGGTCTTGTTCCATTGATGAACTACAAAAGATTGAACAACAGTTAGAGAGAAGTATAAGCAAAATTCGAGCAAGAAAGACTCAGGTTTTCAGGGAACAAATTGAGCAGCTAAAGGAAAAGGAAAAAACCCTAGTTGCTGAAAATGCCATGCTCACAGAGAAGGAAGCAACAAATGATCGGAGAGAGAATATAGCTGAAGCTGAACCTTCTGCAGATCAAAGTAGTCCAAGTTCAGATGTGGAGACTGAATTGTTCATTGGTCTTCCAGAAACAAGAACAAGACGCATTTCTCCAAAGGTGTAG

Protein sequence

>Msa1185320

MVRGKTQMKRIENATSRQVTFSKRRNGLLKKAFELSVLCDAEVALIVFSPRGRLYEFASSSIPETIERYRNHTRINSTPTPSESVENTQQLKEEAENMMKKIDLLETSKRKLLGEGLGSCSIDELQKIEQQLERSISKIRARKTQVFREQIEQLKEKEKTLVAENAMLTEKEATNDRRENIAEAEPSADQSSPSSDVETELFIGLPETRTRRISPKV*