AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1206910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1206910 MtrunA17_Chr7g0243051 88.462 52 6 0 23 74 151 202 8.75e-25 92.4
Msa1206910 MtrunA17_Chr4g0007601 63.462 52 19 0 23 74 135 186 3.13e-15 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1206910 AT3G51780.1 65.385 52 18 0 23 74 154 205 5.72e-16 70.5

Find 90 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGTGGACACAATGTTATT+TGG 0.218175 8_1:+64202429 Msa1206910:three_prime_UTR
GATCTCAAAATAAGAAAATA+TGG 0.234683 8_1:+64200004 Msa1206910:exon
TGGTGGACACAATGTTATTT+GGG 0.250228 8_1:+64202430 Msa1206910:three_prime_UTR
TCGAATGACTCCATATGATA+TGG 0.267394 8_1:+64201649 Msa1206910:CDS
CTCATTCTGATTCAAATTGC+AGG 0.290883 8_1:+64203635 Msa1206910:three_prime_UTR
GTTCAGTAGATCAAATTAAA+AGG 0.312955 8_1:+64203386 Msa1206910:three_prime_UTR
CCTCTTGTCTGTGTTGAAAA+TGG 0.317745 8_1:+64201678 Msa1206910:exon
TAACTGAACTCCCTCCTATT+AGG 0.329209 8_1:+64202267 Msa1206910:three_prime_UTR
AATAGATAAACTAACCTAAT+AGG 0.330539 8_1:-64202281 None:intergenic
AATCCAAGCAAAAGACTCTT+AGG 0.331299 8_1:-64202482 None:intergenic
GCTGGAGCTGGATGGTATTA+AGG 0.331947 8_1:+64200668 Msa1206910:CDS
TTGAAGCAAATCAATGTATC+AGG 0.351192 8_1:-64202064 None:intergenic
TCTTCCCAAACAGTATACTA+TGG 0.363218 8_1:-64203259 None:intergenic
CAATCCTTATGTCGATCTTA+AGG 0.363766 8_1:+64202084 Msa1206910:three_prime_UTR
TAAAACTATAGGGTTTGTTT+TGG 0.367070 8_1:+64202208 Msa1206910:intron
GGTTAAACCTAAAATGATAA+TGG 0.379506 8_1:+64202035 Msa1206910:intron
ATGCTCCATAGTATACTGTT+TGG 0.382079 8_1:+64203254 Msa1206910:three_prime_UTR
ATTAAAGAATGAAATCAAAA+TGG 0.388020 8_1:-64203491 None:intergenic
TGATAAAGAAAGGGAATTCA+TGG 0.391197 8_1:+64203364 Msa1206910:three_prime_UTR
GGTGATTCATGTTTGTTGTC+TGG 0.397207 8_1:+64202229 Msa1206910:three_prime_UTR
AGAAATCGGTGTGCTGCATT+AGG 0.400898 8_1:-64203584 None:intergenic
GCCTTGGATGTGGCTGTCAA+TGG 0.409273 8_1:+64200585 Msa1206910:CDS
GTTGCTTATGAGGAAATTGC+TGG 0.415619 8_1:+64200650 Msa1206910:CDS
TGCTCCATAGTATACTGTTT+GGG 0.420968 8_1:+64203255 Msa1206910:three_prime_UTR
AAGGACATGATGATATCTAT+TGG 0.424202 8_1:+64202103 Msa1206910:three_prime_UTR
ACTTCAAATCATAAAGAAAT+CGG 0.437226 8_1:-64203598 None:intergenic
AAAATAAACAAAGCCAAAAC+AGG 0.438918 8_1:-64203454 None:intergenic
TGACTACAGAGTTGCTTATG+AGG 0.441095 8_1:+64200640 Msa1206910:CDS
ATTTCATTGCTGATAAAGAA+AGG 0.445698 8_1:+64203354 Msa1206910:three_prime_UTR
GAAATTACTGTTCAAGATCC+AGG 0.454111 8_1:-64203336 None:intergenic
CTCCAGAGAGCTTCATCAAA+AGG 0.455289 8_1:-64201792 None:intergenic
ATTTAAATTGTTTCTGCAAG+TGG 0.457730 8_1:+64203698 Msa1206910:three_prime_UTR
GGCGGTCATGCTTGAAGGGT+TGG 0.466973 8_1:+64201699 Msa1206910:three_prime_UTR
GCAGGTGTTGGCCTTGGATG+TGG 0.469671 8_1:+64200575 Msa1206910:intron
CGGCCAAGGTTGCGATTTGA+CGG 0.472968 8_1:+64201878 Msa1206910:three_prime_UTR
AAAATGGCGGTCATGCTTGA+AGG 0.473451 8_1:+64201694 Msa1206910:exon
CAAGAACTGTAAGAATGATC+TGG 0.474307 8_1:-64203772 None:intergenic
AGCATATGCAGGTTGCCATC+AGG 0.476906 8_1:-64203236 None:intergenic
AATGAAAATACGACGGCTCA+TGG 0.476933 8_1:+64201839 Msa1206910:three_prime_UTR
CGAATGACTCCATATGATAT+GGG 0.478606 8_1:+64201650 Msa1206910:CDS
GTCATGCTTGAAGGGTTGGC+AGG 0.484504 8_1:+64201703 Msa1206910:three_prime_UTR
TCTGCAAGTGGAGCCTAGGC+TGG 0.491643 8_1:+64203710 Msa1206910:three_prime_UTR
TATGAGGAAATTGCTGGAGC+TGG 0.493825 8_1:+64200656 Msa1206910:CDS
TTGGATGTGGCTGTCAATGG+AGG 0.496098 8_1:+64200588 Msa1206910:CDS
GGGTTGGCAGGCAATGTCAT+TGG 0.497838 8_1:+64201715 Msa1206910:three_prime_UTR
TTTCATTGCTGATAAAGAAA+GGG 0.511017 8_1:+64203355 Msa1206910:three_prime_UTR
TGTTTCTGCAAGTGGAGCCT+AGG 0.513444 8_1:+64203706 Msa1206910:three_prime_UTR
GAATCAGAATGAGTGTGAAA+AGG 0.516413 8_1:-64203625 None:intergenic
TTCTGATTCAAATTGCAGGT+TGG 0.523285 8_1:+64203639 Msa1206910:three_prime_UTR
ATAAGTCTAATTTCTGACTA+CGG 0.526831 8_1:+64201767 Msa1206910:three_prime_UTR
TTGATGATAAAATAGAATCC+TGG 0.527986 8_1:+64203318 Msa1206910:three_prime_UTR
TTGGCAGGCAATGTCATTGG+AGG 0.531541 8_1:+64201718 Msa1206910:three_prime_UTR
AATATAAATGCTACCAGCCT+AGG 0.534326 8_1:-64203723 None:intergenic
GCCTGCTCCTTCTGTAGAGG+AGG 0.535099 8_1:+64200073 Msa1206910:CDS
ACCTCCTCTACAGAAGGAGC+AGG 0.535547 8_1:-64200074 None:intergenic
AAGAATGATCTGGTTGGTAT+TGG 0.537584 8_1:-64203762 None:intergenic
GTATACTATGGAGCATATGC+AGG 0.541768 8_1:-64203247 None:intergenic
AGATAAACTAACCTAATAGG+AGG 0.545332 8_1:-64202278 None:intergenic
CTGGCCTGCTCCTTCTGTAG+AGG 0.555619 8_1:+64200070 Msa1206910:CDS
GAAAATGTGTCTCACGGCCA+AGG 0.559670 8_1:+64201864 Msa1206910:three_prime_UTR
AACAGCTAATGAAAAGACTC+TGG 0.561222 8_1:+64200051 Msa1206910:CDS
GTAGCATTTATATTATGTCA+AGG 0.562076 8_1:+64203732 Msa1206910:three_prime_UTR
ATGTCCTTAAGATCGACATA+AGG 0.562412 8_1:-64202088 None:intergenic
TAGCATTTATATTATGTCAA+GGG 0.565431 8_1:+64203733 Msa1206910:three_prime_UTR
CAAATAACATTGTGTCCACC+AGG 0.577263 8_1:-64202428 None:intergenic
TTGTGAATCTTGTGAGCAGA+GGG 0.580456 8_1:-64202458 None:intergenic
AACCTAAAATGATAATGGCA+AGG 0.590419 8_1:+64202040 Msa1206910:three_prime_UTR
GGTGGACACAATGTTATTTG+GGG 0.591154 8_1:+64202431 Msa1206910:three_prime_UTR
TTACCGTCAAATCGCAACCT+TGG 0.595041 8_1:-64201881 None:intergenic
GATATTACCTCCTCTACAGA+AGG 0.596493 8_1:-64200080 None:intergenic
AACTGTAAGAATGATCTGGT+TGG 0.596770 8_1:-64203768 None:intergenic
TTAAGGCTGAAGGTGAAGCT+AGG 0.598267 8_1:+64200685 Msa1206910:CDS
CAATATGTAACACTTGCAAG+TGG 0.598595 8_1:+64203532 Msa1206910:three_prime_UTR
TGGATGTGGCTGTCAATGGA+GGG 0.601202 8_1:+64200589 Msa1206910:CDS
CTGGATGGTATTAAGGCTGA+AGG 0.605506 8_1:+64200675 Msa1206910:CDS
GATAAACTAACCTAATAGGA+GGG 0.607158 8_1:-64202277 None:intergenic
AGGAAATTGCTGGAGCTGGA+TGG 0.613422 8_1:+64200660 Msa1206910:CDS
AAATTCTTTGTCAGAAACCG+TGG 0.626027 8_1:-64200613 None:intergenic
ATGGCGGAAAATGTGTCTCA+CGG 0.626613 8_1:+64201858 Msa1206910:three_prime_UTR
GGCTGTCAATGGAGGGACCA+CGG 0.626800 8_1:+64200596 Msa1206910:CDS
TCCATTGACAGCCACATCCA+AGG 0.628433 8_1:-64200586 None:intergenic
AAATGGCGGTCATGCTTGAA+GGG 0.632430 8_1:+64201695 Msa1206910:three_prime_UTR
TAGGTTGCAGAGAAAAGCTG+AGG 0.646000 8_1:+64200704 Msa1206910:CDS
ATTGTGAATCTTGTGAGCAG+AGG 0.647355 8_1:-64202459 None:intergenic
CTTGTCTGTGTTGAAAATGG+CGG 0.653434 8_1:+64201681 Msa1206910:exon
AATTAAAAGGAAGATGACAA+TGG 0.667253 8_1:+64203399 Msa1206910:three_prime_UTR
TTGTCAAAGAGAAAACCTGA+TGG 0.669312 8_1:+64203221 Msa1206910:three_prime_UTR
ATACTTAAATGAAAATACGA+CGG 0.677422 8_1:+64201832 Msa1206910:three_prime_UTR
GAAAATACGACGGCTCATGG+CGG 0.694524 8_1:+64201842 Msa1206910:three_prime_UTR
ACAATTGATAGAGATAAACA+AGG 0.713388 8_1:+64203662 Msa1206910:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGATAATGTTTTATTTA+GGG + chr8_1:64200389-64200408 Msa1206910:intron 10.0%
!!! CATAGTTTTTTTTAAAATAA+CGG - chr8_1:64202733-64202752 None:intergenic 10.0%
!!! TTTAGTTGATAAAAAATTTA+TGG + chr8_1:64202943-64202962 Msa1206910:intron 10.0%
!!! GTAATGATAATGTTTTATTT+AGG + chr8_1:64200388-64200407 Msa1206910:intron 15.0%
!!! CGTTATTTTAAAAAAAACTA+TGG + chr8_1:64202731-64202750 Msa1206910:intron 15.0%
!!! TTTAAAAAAAACTATGGTTT+TGG + chr8_1:64202737-64202756 Msa1206910:intron 15.0%
!!! TAAAATATCGTTCAAATTTT+CGG - chr8_1:64202919-64202938 None:intergenic 15.0%
!!! ATTATATGTATCATGAATTT+TGG - chr8_1:64203062-64203081 None:intergenic 15.0%
!! ATTAAAGAATGAAATCAAAA+TGG - chr8_1:64203494-64203513 None:intergenic 15.0%
!! GATCTCAAAATAAGAAAATA+TGG + chr8_1:64200004-64200023 Msa1206910:exon 20.0%
!!! ATATCAATAATCATCTGTTT+TGG + chr8_1:64200098-64200117 Msa1206910:intron 20.0%
!!! TATCAATAATCATCTGTTTT+GGG + chr8_1:64200099-64200118 Msa1206910:intron 20.0%
!!! AAAATCTTTTAACAAAACCA+TGG - chr8_1:64200419-64200438 None:intergenic 20.0%
!! TAATAATAATATGGGAAACA+TGG - chr8_1:64200495-64200514 None:intergenic 20.0%
!!! TTTAATACCATGATTTTGTT+TGG - chr8_1:64200825-64200844 None:intergenic 20.0%
!!! TTTCGAACTTTTTAAGTTTT+TGG + chr8_1:64200858-64200877 Msa1206910:intron 20.0%
!!! TTTTTAAGTTTTTGGAATCT+GGG + chr8_1:64200866-64200885 Msa1206910:intron 20.0%
!! GGGTTGTTAAAAATTAAAAA+TGG + chr8_1:64200886-64200905 Msa1206910:intron 20.0%
!! AAAAATTAAAAATGGAGTGT+TGG + chr8_1:64200894-64200913 Msa1206910:intron 20.0%
!! TAACTACTACTCAAAATATA+TGG + chr8_1:64201037-64201056 Msa1206910:intron 20.0%
!! ATACTTAAATGAAAATACGA+CGG + chr8_1:64201832-64201851 Msa1206910:three_prime_UTR 20.0%
!! AATAGATAAACTAACCTAAT+AGG - chr8_1:64202284-64202303 None:intergenic 20.0%
!!! TACATAGTTCTTTAAAAACA+TGG - chr8_1:64202642-64202661 None:intergenic 20.0%
!!! ATTTTTATGTTATCAACCAA+AGG - chr8_1:64202991-64203010 None:intergenic 20.0%
!!! GTTGATAACATAAAAATTGA+AGG + chr8_1:64202994-64203013 Msa1206910:intron 20.0%
!!! CTCATAAATTTTATGTATCA+TGG - chr8_1:64203085-64203104 None:intergenic 20.0%
!! CATGATACATAAAATTTATG+AGG + chr8_1:64203083-64203102 Msa1206910:intron 20.0%
!!! ATTGTTGTATTTCTATATAG+TGG + chr8_1:64203124-64203143 Msa1206910:intron 20.0%
!! ACTTCAAATCATAAAGAAAT+CGG - chr8_1:64203601-64203620 None:intergenic 20.0%
!! TAGCATTTATATTATGTCAA+GGG + chr8_1:64203733-64203752 Msa1206910:three_prime_UTR 20.0%
! TTTCTTCTCATGTGTTTATT+TGG - chr8_1:64200245-64200264 None:intergenic 25.0%
!!! CAAAATGATTTTCTGAAAGT+TGG + chr8_1:64200289-64200308 Msa1206910:intron 25.0%
!!! ATTACTGATTGTAATTTTGC+AGG + chr8_1:64200557-64200576 Msa1206910:intron 25.0%
!!! CTTTTTAAGTTTTTGGAATC+TGG + chr8_1:64200865-64200884 Msa1206910:intron 25.0%
!!! GTTTTGAATTTCTTTGACTT+TGG + chr8_1:64201085-64201104 Msa1206910:intron 25.0%
! ACAACAAATCATTAACACAA+GGG - chr8_1:64201132-64201151 None:intergenic 25.0%
! TACAACAAATCATTAACACA+AGG - chr8_1:64201133-64201152 None:intergenic 25.0%
! TTGTTGTAATATGTTTCCAA+TGG + chr8_1:64201145-64201164 Msa1206910:intron 25.0%
!!! AACGAAAATATTTTGACCAT+TGG - chr8_1:64201164-64201183 None:intergenic 25.0%
! AAGTAGGAAAACACATAAAT+AGG - chr8_1:64201305-64201324 None:intergenic 25.0%
! TACACTTGAAACTTAAAAGA+AGG + chr8_1:64201408-64201427 Msa1206910:intron 25.0%
!!! TTCATTATCGTTGATTCTAA+CGG + chr8_1:64201503-64201522 Msa1206910:intron 25.0%
!! ATTTATTGAATGCATTTTGC+AGG + chr8_1:64201619-64201638 Msa1206910:intron 25.0%
! ATAAGTCTAATTTCTGACTA+CGG + chr8_1:64201767-64201786 Msa1206910:three_prime_UTR 25.0%
!!! GTTAAGTTTTTTACTGCTAT+GGG + chr8_1:64201969-64201988 Msa1206910:intron 25.0%
! GGTTAAACCTAAAATGATAA+TGG + chr8_1:64202035-64202054 Msa1206910:intron 25.0%
!!! ACTTATGTCTCTAAAACTAT+AGG + chr8_1:64202197-64202216 Msa1206910:intron 25.0%
!!! CTTATGTCTCTAAAACTATA+GGG + chr8_1:64202198-64202217 Msa1206910:intron 25.0%
!!! TAAAACTATAGGGTTTGTTT+TGG + chr8_1:64202208-64202227 Msa1206910:intron 25.0%
!!! TTTTTTTTAAGACAATCTCC+TGG + chr8_1:64202410-64202429 Msa1206910:intron 25.0%
!!! CTTTAAAAACATGGCAATTT+TGG - chr8_1:64202633-64202652 None:intergenic 25.0%
! TTTAAAGAACTATGTATGCT+TGG + chr8_1:64202646-64202665 Msa1206910:intron 25.0%
!!! AATAACGGTAATTTTAGTCT+TGG - chr8_1:64202718-64202737 None:intergenic 25.0%
!!! AATGACTTTTTTTAGTGTAC+AGG + chr8_1:64202876-64202895 Msa1206910:intron 25.0%
!! TTTATGGCAAGAATTCTTTT+TGG + chr8_1:64202959-64202978 Msa1206910:intron 25.0%
!! TTATGGCAAGAATTCTTTTT+GGG + chr8_1:64202960-64202979 Msa1206910:intron 25.0%
! GAAAAAATGTGAATAGTGAT+TGG + chr8_1:64203294-64203313 Msa1206910:three_prime_UTR 25.0%
!! TTGATGATAAAATAGAATCC+TGG + chr8_1:64203318-64203337 Msa1206910:three_prime_UTR 25.0%
! ATTTCATTGCTGATAAAGAA+AGG + chr8_1:64203354-64203373 Msa1206910:three_prime_UTR 25.0%
! TTTCATTGCTGATAAAGAAA+GGG + chr8_1:64203355-64203374 Msa1206910:three_prime_UTR 25.0%
! GTTCAGTAGATCAAATTAAA+AGG + chr8_1:64203386-64203405 Msa1206910:three_prime_UTR 25.0%
! AATTAAAAGGAAGATGACAA+TGG + chr8_1:64203399-64203418 Msa1206910:three_prime_UTR 25.0%
!! ACAGTTAATTTTACCTGTTT+TGG + chr8_1:64203441-64203460 Msa1206910:three_prime_UTR 25.0%
! AAAATAAACAAAGCCAAAAC+AGG - chr8_1:64203457-64203476 None:intergenic 25.0%
! ACAATTGATAGAGATAAACA+AGG + chr8_1:64203662-64203681 Msa1206910:three_prime_UTR 25.0%
!!! ATTTAAATTGTTTCTGCAAG+TGG + chr8_1:64203698-64203717 Msa1206910:three_prime_UTR 25.0%
! GTAGCATTTATATTATGTCA+AGG + chr8_1:64203732-64203751 Msa1206910:three_prime_UTR 25.0%
! AGCAAGTTATTAGCTTAAGA+AGG - chr8_1:64200326-64200345 None:intergenic 30.0%
AAAAGCTGAGGTAATGTTTA+TGG + chr8_1:64200716-64200735 Msa1206910:intron 30.0%
!! GATTTTGTTTGGCACATATA+TGG - chr8_1:64200814-64200833 None:intergenic 30.0%
ATATGTGCCAAACAAAATCA+TGG + chr8_1:64200815-64200834 Msa1206910:intron 30.0%
!! TTTGATGGTGTTGAAATTCA+TGG + chr8_1:64200979-64200998 Msa1206910:intron 30.0%
TCGCTGTAATTTATGAATTG+AGG + chr8_1:64201063-64201082 Msa1206910:intron 30.0%
! TTTTGATAAGAAAGCGATTC+TGG + chr8_1:64201182-64201201 Msa1206910:intron 30.0%
! CACAACTTATTGATGGTATT+TGG + chr8_1:64201480-64201499 Msa1206910:intron 30.0%
AATATGAAGAGCAGGAAATA+AGG - chr8_1:64201931-64201950 None:intergenic 30.0%
GTAAAAAACTTAACCATCAC+AGG - chr8_1:64201963-64201982 None:intergenic 30.0%
!!! GGTTAAGTTTTTTACTGCTA+TGG + chr8_1:64201968-64201987 Msa1206910:intron 30.0%
CATACTAATTTCCCTAGATT+GGG + chr8_1:64202001-64202020 Msa1206910:intron 30.0%
TTTCATAATCACCCAATCTA+GGG - chr8_1:64202015-64202034 None:intergenic 30.0%
!! AACCTAAAATGATAATGGCA+AGG + chr8_1:64202040-64202059 Msa1206910:three_prime_UTR 30.0%
TTGAAGCAAATCAATGTATC+AGG - chr8_1:64202067-64202086 None:intergenic 30.0%
AAGGACATGATGATATCTAT+TGG + chr8_1:64202103-64202122 Msa1206910:three_prime_UTR 30.0%
GATAAACTAACCTAATAGGA+GGG - chr8_1:64202280-64202299 None:intergenic 30.0%
AGATAAACTAACCTAATAGG+AGG - chr8_1:64202281-64202300 None:intergenic 30.0%
!!! AAAAAAACTATGGTTTTGGC+TGG + chr8_1:64202741-64202760 Msa1206910:intron 30.0%
TTCAAAAGTAACTGCATGTT+AGG - chr8_1:64202857-64202876 None:intergenic 30.0%
AACTATATTAGACAACACTG+AGG - chr8_1:64203168-64203187 None:intergenic 30.0%
TTATGTGTAAACTGTTTCAG+TGG + chr8_1:64203190-64203209 Msa1206910:intron 30.0%
TGATAAAGAAAGGGAATTCA+TGG + chr8_1:64203364-64203383 Msa1206910:three_prime_UTR 30.0%
CATTAGGACAAAAAAACTGT+GGG - chr8_1:64203571-64203590 None:intergenic 30.0%
AACAGCTAATGAAAAGACTC+TGG + chr8_1:64200051-64200070 Msa1206910:CDS 35.0%
! GATTGTAATTTTGCAGGTGT+TGG + chr8_1:64200563-64200582 Msa1206910:intron 35.0%
AAATTCTTTGTCAGAAACCG+TGG - chr8_1:64200616-64200635 None:intergenic 35.0%
!! ATGCAAATTGCAGGTTTTGA+TGG + chr8_1:64200964-64200983 Msa1206910:intron 35.0%
! TGGTTACACAATCTCTGTTT+TGG + chr8_1:64201008-64201027 Msa1206910:intron 35.0%
CCAAAAACATGCAGAAAAGA+GGG - chr8_1:64201261-64201280 None:intergenic 35.0%
ACCAAAAACATGCAGAAAAG+AGG - chr8_1:64201262-64201281 None:intergenic 35.0%
GATAACTCATGTGCGATATT+TGG + chr8_1:64201327-64201346 Msa1206910:intron 35.0%
TCACTTGCACAACTTATTGA+TGG + chr8_1:64201473-64201492 Msa1206910:intron 35.0%
TCGAATGACTCCATATGATA+TGG + chr8_1:64201649-64201668 Msa1206910:CDS 35.0%
CGAATGACTCCATATGATAT+GGG + chr8_1:64201650-64201669 Msa1206910:CDS 35.0%
!!! GTCATTGGAGGTTTTGAATA+TGG + chr8_1:64201730-64201749 Msa1206910:three_prime_UTR 35.0%
TTCCTGCTCTTCATATTGAT+TGG + chr8_1:64201934-64201953 Msa1206910:intron 35.0%
GCATACTAATTTCCCTAGAT+TGG + chr8_1:64202000-64202019 Msa1206910:intron 35.0%
CTTTCATAATCACCCAATCT+AGG - chr8_1:64202016-64202035 None:intergenic 35.0%
CTAGATTGGGTGATTATGAA+AGG + chr8_1:64202014-64202033 Msa1206910:intron 35.0%
! GACCTTGCCATTATCATTTT+AGG - chr8_1:64202045-64202064 None:intergenic 35.0%
CAATCCTTATGTCGATCTTA+AGG + chr8_1:64202084-64202103 Msa1206910:three_prime_UTR 35.0%
ATGTCCTTAAGATCGACATA+AGG - chr8_1:64202091-64202110 None:intergenic 35.0%
!!! TTTTTAAGACAATCTCCTGG+TGG + chr8_1:64202413-64202432 Msa1206910:intron 35.0%
! TGGTGGACACAATGTTATTT+GGG + chr8_1:64202430-64202449 Msa1206910:three_prime_UTR 35.0%
! AATCCTAAGAGTCTTTTGCT+TGG + chr8_1:64202479-64202498 Msa1206910:three_prime_UTR 35.0%
AATCCAAGCAAAAGACTCTT+AGG - chr8_1:64202485-64202504 None:intergenic 35.0%
! AGTCTTTTGCTTGGATTTCT+TGG + chr8_1:64202488-64202507 Msa1206910:three_prime_UTR 35.0%
AGAACTATGTATGCTTGGAT+TGG + chr8_1:64202651-64202670 Msa1206910:intron 35.0%
TGGAACGCGAGAAAATTAAT+TGG - chr8_1:64202698-64202717 None:intergenic 35.0%
TAACTGCATGTTAGGAATCA+CGG - chr8_1:64202849-64202868 None:intergenic 35.0%
!!! CTTTTTTTAGTGTACAGGTG+CGG + chr8_1:64202881-64202900 Msa1206910:intron 35.0%
!!! TTTTTTTAGTGTACAGGTGC+GGG + chr8_1:64202882-64202901 Msa1206910:intron 35.0%
!!! TTCTTTTTGGGTAAAGCCTT+TGG + chr8_1:64202972-64202991 Msa1206910:intron 35.0%
TTGTCAAAGAGAAAACCTGA+TGG + chr8_1:64203221-64203240 Msa1206910:three_prime_UTR 35.0%
ATGCTCCATAGTATACTGTT+TGG + chr8_1:64203254-64203273 Msa1206910:three_prime_UTR 35.0%
TGCTCCATAGTATACTGTTT+GGG + chr8_1:64203255-64203274 Msa1206910:three_prime_UTR 35.0%
TCTTCCCAAACAGTATACTA+TGG - chr8_1:64203262-64203281 None:intergenic 35.0%
GAAATTACTGTTCAAGATCC+AGG - chr8_1:64203339-64203358 None:intergenic 35.0%
CAATATGTAACACTTGCAAG+TGG + chr8_1:64203532-64203551 Msa1206910:three_prime_UTR 35.0%
!!! ACTTGCAAGTGGTTTTGTTT+TGG + chr8_1:64203543-64203562 Msa1206910:three_prime_UTR 35.0%
GCATTAGGACAAAAAAACTG+TGG - chr8_1:64203572-64203591 None:intergenic 35.0%
GAATCAGAATGAGTGTGAAA+AGG - chr8_1:64203628-64203647 None:intergenic 35.0%
CTCATTCTGATTCAAATTGC+AGG + chr8_1:64203635-64203654 Msa1206910:three_prime_UTR 35.0%
TTCTGATTCAAATTGCAGGT+TGG + chr8_1:64203639-64203658 Msa1206910:three_prime_UTR 35.0%
AATATAAATGCTACCAGCCT+AGG - chr8_1:64203726-64203745 None:intergenic 35.0%
! AAGAATGATCTGGTTGGTAT+TGG - chr8_1:64203765-64203784 None:intergenic 35.0%
AACTGTAAGAATGATCTGGT+TGG - chr8_1:64203771-64203790 None:intergenic 35.0%
CAAGAACTGTAAGAATGATC+TGG - chr8_1:64203775-64203794 None:intergenic 35.0%
GATATTACCTCCTCTACAGA+AGG - chr8_1:64200083-64200102 None:intergenic 40.0%
!!! GTTTTATTTAGGGTGAGCCA+TGG + chr8_1:64200399-64200418 Msa1206910:intron 40.0%
TTTCAAGCTGTTGCTGTAGT+AGG + chr8_1:64200436-64200455 Msa1206910:intron 40.0%
TGACTACAGAGTTGCTTATG+AGG + chr8_1:64200640-64200659 Msa1206910:CDS 40.0%
GTTGCTTATGAGGAAATTGC+TGG + chr8_1:64200650-64200669 Msa1206910:CDS 40.0%
!! ATGGAGTGTTGGAATAGTGT+TGG + chr8_1:64200905-64200924 Msa1206910:intron 40.0%
CTCAATGTGATGCAAATTGC+AGG + chr8_1:64200955-64200974 Msa1206910:intron 40.0%
GTTGAAATTCATGGAGCTCA+TGG + chr8_1:64200988-64201007 Msa1206910:intron 40.0%
TAAGAAAGCGATTCTGGTGA+AGG + chr8_1:64201188-64201207 Msa1206910:intron 40.0%
! CCCTCTTTTCTGCATGTTTT+TGG + chr8_1:64201258-64201277 Msa1206910:intron 40.0%
! GCACATGAGTTATCTGAAGT+AGG - chr8_1:64201321-64201340 None:intergenic 40.0%
!! TCATGTGCGATATTTGGTGT+GGG + chr8_1:64201333-64201352 Msa1206910:intron 40.0%
! GAGGCTTTTCCCATATCATA+TGG - chr8_1:64201662-64201681 None:intergenic 40.0%
! CCATTTTCAACACAGACAAG+AGG - chr8_1:64201681-64201700 None:intergenic 40.0%
CCTCTTGTCTGTGTTGAAAA+TGG + chr8_1:64201678-64201697 Msa1206910:exon 40.0%
CTTGTCTGTGTTGAAAATGG+CGG + chr8_1:64201681-64201700 Msa1206910:exon 40.0%
AATGAAAATACGACGGCTCA+TGG + chr8_1:64201839-64201858 Msa1206910:three_prime_UTR 40.0%
CACCAATCAATATGAAGAGC+AGG - chr8_1:64201939-64201958 None:intergenic 40.0%
! TATTGATTGGTGACCTGTGA+TGG + chr8_1:64201947-64201966 Msa1206910:intron 40.0%
!! GGTGATTCATGTTTGTTGTC+TGG + chr8_1:64202229-64202248 Msa1206910:three_prime_UTR 40.0%
TAACTGAACTCCCTCCTATT+AGG + chr8_1:64202267-64202286 Msa1206910:three_prime_UTR 40.0%
!! CTCACAAGTTTGTTGTCACA+TGG + chr8_1:64202374-64202393 Msa1206910:intron 40.0%
!! CAAGTTTGTTGTCACATGGT+TGG + chr8_1:64202378-64202397 Msa1206910:intron 40.0%
CAAATAACATTGTGTCCACC+AGG - chr8_1:64202431-64202450 None:intergenic 40.0%
! CTGGTGGACACAATGTTATT+TGG + chr8_1:64202429-64202448 Msa1206910:three_prime_UTR 40.0%
! GGTGGACACAATGTTATTTG+GGG + chr8_1:64202431-64202450 Msa1206910:three_prime_UTR 40.0%
TTGTGAATCTTGTGAGCAGA+GGG - chr8_1:64202461-64202480 None:intergenic 40.0%
ATTGTGAATCTTGTGAGCAG+AGG - chr8_1:64202462-64202481 None:intergenic 40.0%
! ACTATGTATGCTTGGATTGG+CGG + chr8_1:64202654-64202673 Msa1206910:intron 40.0%
!!! AAAACTATGGTTTTGGCTGG+CGG + chr8_1:64202744-64202763 Msa1206910:intron 40.0%
! GCAGTGAACCATTGTGATTT+TGG + chr8_1:64202778-64202797 Msa1206910:intron 40.0%
GTATACTATGGAGCATATGC+AGG - chr8_1:64203250-64203269 None:intergenic 40.0%
TGTTGCTGTAGTAGGATCAG+AGG + chr8_1:64200444-64200463 Msa1206910:intron 45.0%
!!! AATTTTGCAGGTGTTGGCCT+TGG + chr8_1:64200569-64200588 Msa1206910:intron 45.0%
TATGAGGAAATTGCTGGAGC+TGG + chr8_1:64200656-64200675 Msa1206910:CDS 45.0%
CTGGATGGTATTAAGGCTGA+AGG + chr8_1:64200675-64200694 Msa1206910:CDS 45.0%
TTAAGGCTGAAGGTGAAGCT+AGG + chr8_1:64200685-64200704 Msa1206910:CDS 45.0%
TAGGTTGCAGAGAAAAGCTG+AGG + chr8_1:64200704-64200723 Msa1206910:CDS 45.0%
!! GAGTGTTGGAATAGTGTTGG+TGG + chr8_1:64200908-64200927 Msa1206910:intron 45.0%
! CTCATGTGCGATATTTGGTG+TGG + chr8_1:64201332-64201351 Msa1206910:intron 45.0%
AAAATGGCGGTCATGCTTGA+AGG + chr8_1:64201694-64201713 Msa1206910:exon 45.0%
AAATGGCGGTCATGCTTGAA+GGG + chr8_1:64201695-64201714 Msa1206910:three_prime_UTR 45.0%
! AGCCTTTTGATGAAGCTCTC+TGG + chr8_1:64201790-64201809 Msa1206910:three_prime_UTR 45.0%
CTCCAGAGAGCTTCATCAAA+AGG - chr8_1:64201795-64201814 None:intergenic 45.0%
! ATGGCGGAAAATGTGTCTCA+CGG + chr8_1:64201858-64201877 Msa1206910:three_prime_UTR 45.0%
TTACCGTCAAATCGCAACCT+TGG - chr8_1:64201884-64201903 None:intergenic 45.0%
!! AGCGTTGGCCAAAATCACAA+TGG - chr8_1:64202789-64202808 None:intergenic 45.0%
! TTTTGGCCAACGCTACACTT+TGG + chr8_1:64202795-64202814 Msa1206910:intron 45.0%
TAAGCTCCAAAGTGTAGCGT+TGG - chr8_1:64202804-64202823 None:intergenic 45.0%
AGGAATCACGGTGAGTTTCA+CGG - chr8_1:64202837-64202856 None:intergenic 45.0%
AGAAATCGGTGTGCTGCATT+AGG - chr8_1:64203587-64203606 None:intergenic 45.0%
!! ATAATGAATAATAATAATAT+GGG - chr8_1:64200503-64200522 None:intergenic 5.0%
!! TATAATGAATAATAATAATA+TGG - chr8_1:64200504-64200523 None:intergenic 5.0%
TGCTGTAGTAGGATCAGAGG+TGG + chr8_1:64200447-64200466 Msa1206910:intron 50.0%
! AGGTGGACAAGCTTTGTGAC+AGG + chr8_1:64200464-64200483 Msa1206910:intron 50.0%
! GGTGGACAAGCTTTGTGACA+GGG + chr8_1:64200465-64200484 Msa1206910:intron 50.0%
TCCATTGACAGCCACATCCA+AGG - chr8_1:64200589-64200608 None:intergenic 50.0%
TTGGATGTGGCTGTCAATGG+AGG + chr8_1:64200588-64200607 Msa1206910:CDS 50.0%
TGGATGTGGCTGTCAATGGA+GGG + chr8_1:64200589-64200608 Msa1206910:CDS 50.0%
AGGAAATTGCTGGAGCTGGA+TGG + chr8_1:64200660-64200679 Msa1206910:CDS 50.0%
GCTGGAGCTGGATGGTATTA+AGG + chr8_1:64200668-64200687 Msa1206910:CDS 50.0%
! AGCGATTCTGGTGAAGGTGT+CGG + chr8_1:64201194-64201213 Msa1206910:intron 50.0%
CATGCAGAAAAGAGGGACGA+TGG - chr8_1:64201254-64201273 None:intergenic 50.0%
TTGGCAGGCAATGTCATTGG+AGG + chr8_1:64201718-64201737 Msa1206910:three_prime_UTR 50.0%
GAAAATACGACGGCTCATGG+CGG + chr8_1:64201842-64201861 Msa1206910:three_prime_UTR 50.0%
GAAAATGTGTCTCACGGCCA+AGG + chr8_1:64201864-64201883 Msa1206910:three_prime_UTR 50.0%
AGCATATGCAGGTTGCCATC+AGG - chr8_1:64203239-64203258 None:intergenic 50.0%
! TGTTTCTGCAAGTGGAGCCT+AGG + chr8_1:64203706-64203725 Msa1206910:three_prime_UTR 50.0%
ACCTCCTCTACAGAAGGAGC+AGG - chr8_1:64200077-64200096 None:intergenic 55.0%
GCCTTGGATGTGGCTGTCAA+TGG + chr8_1:64200585-64200604 Msa1206910:CDS 55.0%
!! GATTCTGGTGAAGGTGTCGG+CGG + chr8_1:64201197-64201216 Msa1206910:intron 55.0%
! GTCGGCGGAGAGGAGTTTTA+CGG + chr8_1:64201212-64201231 Msa1206910:intron 55.0%
! GTCATGCTTGAAGGGTTGGC+AGG + chr8_1:64201703-64201722 Msa1206910:three_prime_UTR 55.0%
! GGGTTGGCAGGCAATGTCAT+TGG + chr8_1:64201715-64201734 Msa1206910:three_prime_UTR 55.0%
CGGCCAAGGTTGCGATTTGA+CGG + chr8_1:64201878-64201897 Msa1206910:three_prime_UTR 55.0%
!!! ACTATGGTTTTGGCTGGCGG+TGG + chr8_1:64202747-64202766 Msa1206910:intron 55.0%
!!! CTATGGTTTTGGCTGGCGGT+GGG + chr8_1:64202748-64202767 Msa1206910:intron 55.0%
CTGGCCTGCTCCTTCTGTAG+AGG + chr8_1:64200070-64200089 Msa1206910:CDS 60.0%
GCCTGCTCCTTCTGTAGAGG+AGG + chr8_1:64200073-64200092 Msa1206910:CDS 60.0%
!! GCAGGTGTTGGCCTTGGATG+TGG + chr8_1:64200575-64200594 Msa1206910:intron 60.0%
GGCTGTCAATGGAGGGACCA+CGG + chr8_1:64200596-64200615 Msa1206910:CDS 60.0%
! GGCGGAGAGGAGTTTTACGG+TGG + chr8_1:64201215-64201234 Msa1206910:intron 60.0%
GGCGGTCATGCTTGAAGGGT+TGG + chr8_1:64201699-64201718 Msa1206910:three_prime_UTR 60.0%
TCTGCAAGTGGAGCCTAGGC+TGG + chr8_1:64203710-64203729 Msa1206910:three_prime_UTR 60.0%
!! TGGTGAAGGTGTCGGCGGAG+AGG + chr8_1:64201202-64201221 Msa1206910:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 64199893 64203794 64199893 ID=Msa1206910;Name=Msa1206910
chr8_1 mRNA 64199893 64203794 64199893 ID=Msa1206910-mRNA-1;Parent=Msa1206910;Name=Msa1206910-mRNA-1;_AED=0.05;_eAED=0.05;_QI=130|0.66|1|1|0.33|0.57|7|1038|90
chr8_1 exon 64199893 64200094 64199893 ID=Msa1206910-mRNA-1:exon:20924;Parent=Msa1206910-mRNA-1
chr8_1 exon 64200579 64200725 64200579 ID=Msa1206910-mRNA-1:exon:20925;Parent=Msa1206910-mRNA-1
chr8_1 exon 64201641 64201899 64201641 ID=Msa1206910-mRNA-1:exon:20926;Parent=Msa1206910-mRNA-1
chr8_1 exon 64202036 64202124 64202036 ID=Msa1206910-mRNA-1:exon:20927;Parent=Msa1206910-mRNA-1
chr8_1 exon 64202219 64202288 64202219 ID=Msa1206910-mRNA-1:exon:20928;Parent=Msa1206910-mRNA-1
chr8_1 exon 64202421 64202509 64202421 ID=Msa1206910-mRNA-1:exon:20929;Parent=Msa1206910-mRNA-1
chr8_1 exon 64203210 64203794 64203210 ID=Msa1206910-mRNA-1:exon:20930;Parent=Msa1206910-mRNA-1
chr8_1 five_prime_UTR 64199893 64200022 64199893 ID=Msa1206910-mRNA-1:five_prime_utr;Parent=Msa1206910-mRNA-1
chr8_1 CDS 64200023 64200094 64200023 ID=Msa1206910-mRNA-1:cds;Parent=Msa1206910-mRNA-1
chr8_1 CDS 64200579 64200725 64200579 ID=Msa1206910-mRNA-1:cds;Parent=Msa1206910-mRNA-1
chr8_1 CDS 64201641 64201694 64201641 ID=Msa1206910-mRNA-1:cds;Parent=Msa1206910-mRNA-1
chr8_1 three_prime_UTR 64201695 64201899 64201695 ID=Msa1206910-mRNA-1:three_prime_utr;Parent=Msa1206910-mRNA-1
chr8_1 three_prime_UTR 64202036 64202124 64202036 ID=Msa1206910-mRNA-1:three_prime_utr;Parent=Msa1206910-mRNA-1
chr8_1 three_prime_UTR 64202219 64202288 64202219 ID=Msa1206910-mRNA-1:three_prime_utr;Parent=Msa1206910-mRNA-1
chr8_1 three_prime_UTR 64202421 64202509 64202421 ID=Msa1206910-mRNA-1:three_prime_utr;Parent=Msa1206910-mRNA-1
chr8_1 three_prime_UTR 64203210 64203794 64203210 ID=Msa1206910-mRNA-1:three_prime_utr;Parent=Msa1206910-mRNA-1
Gene Sequence

>Msa1206910

ATGGTTGAGTATCAGAGTGCTGATCATGAACAGCTAATGAAAAGACTCTGGCCTGCTCCTTCTGTAGAGGAGGTGTTGGCCTTGGATGTGGCTGTCAATGGAGGGACCACGGTTTCTGACAAAGAATTTATTGTGACTACAGAGTTGCTTATGAGGAAATTGCTGGAGCTGGATGGTATTAAGGCTGAAGGTGAAGCTAGGTTGCAGAGAAAAGCTGAGGTATTTGCTCGAATGACTCCATATGATATGGGAAAAGCCTCTTGTCTGTGTTGA
Protein sequence

>Msa1206910

MVEYQSADHEQLMKRLWPAPSVEEVLALDVAVNGGTTVSDKEFIVTTELLMRKLLELDGIKAEGEARLQRKAEVFARMTPYDMGKASCLC*