AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1218150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218150 MtrunA17_Chr4g0067831 90.612 245 23 0 1 245 1 245 2.83e-161 446
Msa1218150 MtrunA17_Chr3g0115421 37.288 59 37 0 7 65 28 86 5.21e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218150 AT1G29100.2 29.213 89 63 0 6 94 4 92 2.96e-11 60.5
Msa1218150 AT1G29100.1 29.070 86 61 0 9 94 1 86 8.65e-11 58.9

Find 65 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAGTATCATTCAAAATTT+AGG 0.207400 8_1:-80722485 Msa1218150:three_prime_UTR
CGACAAAGGCTAACAGTTAT+TGG 0.306127 8_1:-80723185 Msa1218150:CDS
TTCAATATTGGAACAAATAA+TGG 0.318901 8_1:+80723103 None:intergenic
TATGGTATAATCTGTTTCTA+TGG 0.323340 8_1:+80722823 None:intergenic
TGAAGTTGTAGGTTCAATAT+TGG 0.323646 8_1:+80723091 None:intergenic
CATGTTAACAGAATTGGTTC+TGG 0.333825 8_1:-80722861 Msa1218150:CDS
CCTAACCATGTTAACAGAAT+TGG 0.338122 8_1:-80722867 Msa1218150:CDS
GTCTCTCTCCTCTATGTCTA+AGG 0.364022 8_1:+80722560 None:intergenic
AGTTAATAAGCACAGAAGAT+AGG 0.365330 8_1:+80722400 None:intergenic
GTAGAAATACAGGGTCATTA+TGG 0.366375 8_1:+80722805 None:intergenic
ATGTCACAGAATCATGATAT+TGG 0.385727 8_1:+80722505 None:intergenic
GAATCTTTAGATGAAGTTGT+AGG 0.385930 8_1:+80723080 None:intergenic
GATGGCTCTTGTAGAAATAC+AGG 0.396677 8_1:+80722795 None:intergenic
CTCGAGGTGTCTGCATTTGA+TGG 0.408504 8_1:+80723399 None:intergenic
CATGTAGGTGTTGGTGATGA+TGG 0.418378 8_1:+80722969 None:intergenic
GTTGCCTCAACCATTGGTTC+TGG 0.423380 8_1:+80722993 None:intergenic
AGTTTGCAATAGAAGTAAGA+AGG 0.451270 8_1:-80722441 Msa1218150:three_prime_UTR
CAGCAGTGGCAGAACAATCT+AGG 0.455141 8_1:-80722933 Msa1218150:CDS
CCAATTCTGTTAACATGGTT+AGG 0.456310 8_1:+80722867 None:intergenic
TATGTATGACATTCAATGGT+TGG 0.458995 8_1:+80722766 None:intergenic
AAAGCACTCAATGGCATCAA+TGG 0.463601 8_1:-80723323 Msa1218150:intron
ATGGATCAGCCACTCTTACA+TGG 0.465348 8_1:+80723418 None:intergenic
AAGGAGGTTCCTTGAGCTTG+TGG 0.468534 8_1:+80723017 None:intergenic
GTTAATAAGCACAGAAGATA+GGG 0.468859 8_1:+80722401 None:intergenic
GTTTGCAATAGAAGTAAGAA+GGG 0.471127 8_1:-80722440 Msa1218150:three_prime_UTR
TAAGGCACTATACTAGACTA+TGG 0.479471 8_1:+80722578 None:intergenic
TGTCAAAGCAATTAAGAAGA+AGG 0.487879 8_1:-80723141 Msa1218150:CDS
ATGTTAACAGAATTGGTTCT+GGG 0.488978 8_1:-80722860 Msa1218150:CDS
CTAGACTATGGTACATGCAT+TGG 0.493223 8_1:+80722590 None:intergenic
CAGAACCAATTCTGTTAACA+TGG 0.495361 8_1:+80722862 None:intergenic
CGCAAAACAGAAACATGCTC+AGG 0.497022 8_1:-80722308 Msa1218150:three_prime_UTR
GATGGTGTTGCCTCAACCAT+TGG 0.497219 8_1:+80722987 None:intergenic
TAACTATGTATGACATTCAA+TGG 0.498996 8_1:+80722762 None:intergenic
TAGACTATGGTACATGCATT+GGG 0.499389 8_1:+80722591 None:intergenic
TGTCTTGCATTGTGCCATGT+AGG 0.515576 8_1:+80722954 None:intergenic
AAAGGCTAACAGTTATTGGA+TGG 0.538223 8_1:-80723181 Msa1218150:CDS
AGACACTACATGAAAAGTAA+AGG 0.538953 8_1:-80722541 Msa1218150:three_prime_UTR
CATCATCACCAACACCTACA+TGG 0.540194 8_1:-80722968 Msa1218150:CDS
TATTCAGTGATATGAGATGA+TGG 0.549737 8_1:+80722726 None:intergenic
AAGATCAAGAAAGCACTCAA+TGG 0.551774 8_1:-80723332 Msa1218150:CDS
CTCAACCATTGGTTCTGGAA+AGG 0.553516 8_1:+80722998 None:intergenic
CTCATATGTTACACCATCAT+CGG 0.560041 8_1:-80722890 Msa1218150:CDS
CTTTCCAGAACCAATGGTTG+AGG 0.566197 8_1:-80722997 Msa1218150:CDS
AACCTCCTTTCCAGAACCAA+TGG 0.569484 8_1:-80723003 Msa1218150:CDS
GTATAGTGCCTTAGACATAG+AGG 0.571229 8_1:-80722568 Msa1218150:three_prime_UTR
CACATCCGTTACAATCAACT+CGG 0.572623 8_1:+80723360 None:intergenic
TTGTAATGTGTGTGCCATGA+TGG 0.573329 8_1:+80722687 None:intergenic
AACCATTGGTTCTGGAAAGG+AGG 0.576590 8_1:+80723001 None:intergenic
TCGTTGACTTCGATCGACAA+AGG 0.580733 8_1:-80723199 Msa1218150:CDS
GCATTGTGCCATGTAGGTGT+TGG 0.586636 8_1:+80722960 None:intergenic
AGGAACTGAAGATGCAGAAG+AGG 0.588018 8_1:-80722913 Msa1218150:CDS
CTTCAAGCTCCACAAGCTCA+AGG 0.591533 8_1:-80723026 Msa1218150:CDS
ACTTGTATCGTTGTGACTCG+AGG 0.595070 8_1:+80723383 None:intergenic
TGGATCAGCCACTCTTACAT+GGG 0.605123 8_1:+80723419 None:intergenic
GGGATTGTCATCGCTGAACA+TGG 0.605508 8_1:+80722611 None:intergenic
TAACATGGTTAGGCCGATGA+TGG 0.610686 8_1:+80722877 None:intergenic
CAAGTCCGAGTTGATTGTAA+CGG 0.616785 8_1:-80723365 Msa1218150:CDS
GCAAGCATCCCATGTAAGAG+TGG 0.623377 8_1:-80723427 Msa1218150:five_prime_UTR
AAGGCTAACAGTTATTGGAT+GGG 0.626092 8_1:-80723180 Msa1218150:CDS
AATATTGGAACAAATAATGG+TGG 0.628109 8_1:+80723106 None:intergenic
AATGTGTTAGATCACCATCA+TGG 0.630608 8_1:-80722701 Msa1218150:CDS
GGCACAATGCAAGACAGCAG+TGG 0.669598 8_1:-80722947 Msa1218150:CDS
ATGGCTCTTGTAGAAATACA+GGG 0.673754 8_1:+80722796 None:intergenic
TGAAATACTTATCAAACACG+AGG 0.689171 8_1:-80722246 None:intergenic
AACTATATAGAACACTACAG+TGG 0.711693 8_1:-80722666 Msa1218150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTTTGTGTGATTAATTA+TGG - chr8_1:80722256-80722275 Msa1218150:three_prime_UTR 20.0%
!! TTCAATATTGGAACAAATAA+TGG + chr8_1:80722628-80722647 None:intergenic 20.0%
!! CATAGTATCATTCAAAATTT+AGG - chr8_1:80723243-80723262 Msa1218150:intron 20.0%
!!! CTTTTTTGTGTGATTAATTA+TGG - chr8_1:80722256-80722275 Msa1218150:three_prime_UTR 20.0%
!! TTCAATATTGGAACAAATAA+TGG + chr8_1:80722628-80722647 None:intergenic 20.0%
!! CATAGTATCATTCAAAATTT+AGG - chr8_1:80723243-80723262 Msa1218150:intron 20.0%
!! ATTGCTTTGACAATATTTTC+TGG + chr8_1:80722579-80722598 None:intergenic 25.0%
! AATATTGGAACAAATAATGG+TGG + chr8_1:80722625-80722644 None:intergenic 25.0%
!!! TGAAGATTTTCTTTTGGTTT+TGG + chr8_1:80722687-80722706 None:intergenic 25.0%
!!! TATGGTATAATCTGTTTCTA+TGG + chr8_1:80722908-80722927 None:intergenic 25.0%
! TAACTATGTATGACATTCAA+TGG + chr8_1:80722969-80722988 None:intergenic 25.0%
!! ATTGCTTTGACAATATTTTC+TGG + chr8_1:80722579-80722598 None:intergenic 25.0%
! AATATTGGAACAAATAATGG+TGG + chr8_1:80722625-80722644 None:intergenic 25.0%
!!! TGAAGATTTTCTTTTGGTTT+TGG + chr8_1:80722687-80722706 None:intergenic 25.0%
!!! TATGGTATAATCTGTTTCTA+TGG + chr8_1:80722908-80722927 None:intergenic 25.0%
! TAACTATGTATGACATTCAA+TGG + chr8_1:80722969-80722988 None:intergenic 25.0%
CAACAAGTTCACAAAATAGT+TGG + chr8_1:80722486-80722505 None:intergenic 30.0%
TGTCAAAGCAATTAAGAAGA+AGG - chr8_1:80722587-80722606 Msa1218150:exon 30.0%
TGAAGTTGTAGGTTCAATAT+TGG + chr8_1:80722640-80722659 None:intergenic 30.0%
GAATCTTTAGATGAAGTTGT+AGG + chr8_1:80722651-80722670 None:intergenic 30.0%
!!! TCTTTTGGTTTTGGTTTTGT+TGG + chr8_1:80722678-80722697 None:intergenic 30.0%
! ATGTTAACAGAATTGGTTCT+GGG - chr8_1:80722868-80722887 Msa1218150:CDS 30.0%
! TTCAATGGTTGGATTTTAGA+TGG + chr8_1:80722954-80722973 None:intergenic 30.0%
TATGTATGACATTCAATGGT+TGG + chr8_1:80722965-80722984 None:intergenic 30.0%
TATTCAGTGATATGAGATGA+TGG + chr8_1:80723005-80723024 None:intergenic 30.0%
AACTATATAGAACACTACAG+TGG - chr8_1:80723062-80723081 Msa1218150:CDS 30.0%
AGACACTACATGAAAAGTAA+AGG - chr8_1:80723187-80723206 Msa1218150:CDS 30.0%
ATGTCACAGAATCATGATAT+TGG + chr8_1:80723226-80723245 None:intergenic 30.0%
AGTTTGCAATAGAAGTAAGA+AGG - chr8_1:80723287-80723306 Msa1218150:intron 30.0%
GTTTGCAATAGAAGTAAGAA+GGG - chr8_1:80723288-80723307 Msa1218150:intron 30.0%
GTTAATAAGCACAGAAGATA+GGG + chr8_1:80723330-80723349 None:intergenic 30.0%
AGTTAATAAGCACAGAAGAT+AGG + chr8_1:80723331-80723350 None:intergenic 30.0%
CAACAAGTTCACAAAATAGT+TGG + chr8_1:80722486-80722505 None:intergenic 30.0%
TGTCAAAGCAATTAAGAAGA+AGG - chr8_1:80722587-80722606 Msa1218150:exon 30.0%
TGAAGTTGTAGGTTCAATAT+TGG + chr8_1:80722640-80722659 None:intergenic 30.0%
GAATCTTTAGATGAAGTTGT+AGG + chr8_1:80722651-80722670 None:intergenic 30.0%
!!! TCTTTTGGTTTTGGTTTTGT+TGG + chr8_1:80722678-80722697 None:intergenic 30.0%
! ATGTTAACAGAATTGGTTCT+GGG - chr8_1:80722868-80722887 Msa1218150:CDS 30.0%
! TTCAATGGTTGGATTTTAGA+TGG + chr8_1:80722954-80722973 None:intergenic 30.0%
TATGTATGACATTCAATGGT+TGG + chr8_1:80722965-80722984 None:intergenic 30.0%
TATTCAGTGATATGAGATGA+TGG + chr8_1:80723005-80723024 None:intergenic 30.0%
AACTATATAGAACACTACAG+TGG - chr8_1:80723062-80723081 Msa1218150:CDS 30.0%
AGACACTACATGAAAAGTAA+AGG - chr8_1:80723187-80723206 Msa1218150:CDS 30.0%
ATGTCACAGAATCATGATAT+TGG + chr8_1:80723226-80723245 None:intergenic 30.0%
AGTTTGCAATAGAAGTAAGA+AGG - chr8_1:80723287-80723306 Msa1218150:intron 30.0%
GTTTGCAATAGAAGTAAGAA+GGG - chr8_1:80723288-80723307 Msa1218150:intron 30.0%
GTTAATAAGCACAGAAGATA+GGG + chr8_1:80723330-80723349 None:intergenic 30.0%
AGTTAATAAGCACAGAAGAT+AGG + chr8_1:80723331-80723350 None:intergenic 30.0%
!! AAGATCAAGAAAGCACTCAA+TGG - chr8_1:80722396-80722415 Msa1218150:three_prime_UTR 35.0%
! TTGCTGGTGTGTTATTAAGA+AGG + chr8_1:80722457-80722476 None:intergenic 35.0%
! AAATAGTTGGTTGATGTTGC+TGG + chr8_1:80722473-80722492 None:intergenic 35.0%
!!! TTGTGAACTTGTTGTTTTGC+AGG - chr8_1:80722492-80722511 Msa1218150:three_prime_UTR 35.0%
AAAGGCTAACAGTTATTGGA+TGG - chr8_1:80722547-80722566 Msa1218150:three_prime_UTR 35.0%
AAGGCTAACAGTTATTGGAT+GGG - chr8_1:80722548-80722567 Msa1218150:three_prime_UTR 35.0%
! GGAGCTTGAAGATTTTCTTT+TGG + chr8_1:80722693-80722712 None:intergenic 35.0%
CTCATATGTTACACCATCAT+CGG - chr8_1:80722838-80722857 Msa1218150:CDS 35.0%
CCAATTCTGTTAACATGGTT+AGG + chr8_1:80722864-80722883 None:intergenic 35.0%
CCTAACCATGTTAACAGAAT+TGG - chr8_1:80722861-80722880 Msa1218150:CDS 35.0%
CAGAACCAATTCTGTTAACA+TGG + chr8_1:80722869-80722888 None:intergenic 35.0%
! CATGTTAACAGAATTGGTTC+TGG - chr8_1:80722867-80722886 Msa1218150:CDS 35.0%
GTAGAAATACAGGGTCATTA+TGG + chr8_1:80722926-80722945 None:intergenic 35.0%
ATGGCTCTTGTAGAAATACA+GGG + chr8_1:80722935-80722954 None:intergenic 35.0%
AATGTGTTAGATCACCATCA+TGG - chr8_1:80723027-80723046 Msa1218150:CDS 35.0%
TAGACTATGGTACATGCATT+GGG + chr8_1:80723140-80723159 None:intergenic 35.0%
! TAAGGCACTATACTAGACTA+TGG + chr8_1:80723153-80723172 None:intergenic 35.0%
!!! TGCTTTTTTGCTTTGACGAA+AGG + chr8_1:80723368-80723387 None:intergenic 35.0%
! GTTTCTGTTTTGCGAAATTC+AGG + chr8_1:80723414-80723433 None:intergenic 35.0%
!! AAGATCAAGAAAGCACTCAA+TGG - chr8_1:80722396-80722415 Msa1218150:three_prime_UTR 35.0%
! TTGCTGGTGTGTTATTAAGA+AGG + chr8_1:80722457-80722476 None:intergenic 35.0%
! AAATAGTTGGTTGATGTTGC+TGG + chr8_1:80722473-80722492 None:intergenic 35.0%
!!! TTGTGAACTTGTTGTTTTGC+AGG - chr8_1:80722492-80722511 Msa1218150:three_prime_UTR 35.0%
AAAGGCTAACAGTTATTGGA+TGG - chr8_1:80722547-80722566 Msa1218150:three_prime_UTR 35.0%
AAGGCTAACAGTTATTGGAT+GGG - chr8_1:80722548-80722567 Msa1218150:three_prime_UTR 35.0%
! GGAGCTTGAAGATTTTCTTT+TGG + chr8_1:80722693-80722712 None:intergenic 35.0%
CTCATATGTTACACCATCAT+CGG - chr8_1:80722838-80722857 Msa1218150:CDS 35.0%
CCAATTCTGTTAACATGGTT+AGG + chr8_1:80722864-80722883 None:intergenic 35.0%
CCTAACCATGTTAACAGAAT+TGG - chr8_1:80722861-80722880 Msa1218150:CDS 35.0%
CAGAACCAATTCTGTTAACA+TGG + chr8_1:80722869-80722888 None:intergenic 35.0%
! CATGTTAACAGAATTGGTTC+TGG - chr8_1:80722867-80722886 Msa1218150:CDS 35.0%
GTAGAAATACAGGGTCATTA+TGG + chr8_1:80722926-80722945 None:intergenic 35.0%
ATGGCTCTTGTAGAAATACA+GGG + chr8_1:80722935-80722954 None:intergenic 35.0%
AATGTGTTAGATCACCATCA+TGG - chr8_1:80723027-80723046 Msa1218150:CDS 35.0%
TAGACTATGGTACATGCATT+GGG + chr8_1:80723140-80723159 None:intergenic 35.0%
! TAAGGCACTATACTAGACTA+TGG + chr8_1:80723153-80723172 None:intergenic 35.0%
!!! TGCTTTTTTGCTTTGACGAA+AGG + chr8_1:80723368-80723387 None:intergenic 35.0%
! GTTTCTGTTTTGCGAAATTC+AGG + chr8_1:80723414-80723433 None:intergenic 35.0%
!! CAAGTCCGAGTTGATTGTAA+CGG - chr8_1:80722363-80722382 Msa1218150:three_prime_UTR 40.0%
CACATCCGTTACAATCAACT+CGG + chr8_1:80722371-80722390 None:intergenic 40.0%
! AAAGCACTCAATGGCATCAA+TGG - chr8_1:80722405-80722424 Msa1218150:three_prime_UTR 40.0%
CGACAAAGGCTAACAGTTAT+TGG - chr8_1:80722543-80722562 Msa1218150:three_prime_UTR 40.0%
GATGGCTCTTGTAGAAATAC+AGG + chr8_1:80722936-80722955 None:intergenic 40.0%
TTGTAATGTGTGTGCCATGA+TGG + chr8_1:80723044-80723063 None:intergenic 40.0%
CTAGACTATGGTACATGCAT+TGG + chr8_1:80723141-80723160 None:intergenic 40.0%
GTATAGTGCCTTAGACATAG+AGG - chr8_1:80723160-80723179 Msa1218150:CDS 40.0%
!! CAAGTCCGAGTTGATTGTAA+CGG - chr8_1:80722363-80722382 Msa1218150:three_prime_UTR 40.0%
CACATCCGTTACAATCAACT+CGG + chr8_1:80722371-80722390 None:intergenic 40.0%
! AAAGCACTCAATGGCATCAA+TGG - chr8_1:80722405-80722424 Msa1218150:three_prime_UTR 40.0%
CGACAAAGGCTAACAGTTAT+TGG - chr8_1:80722543-80722562 Msa1218150:three_prime_UTR 40.0%
GATGGCTCTTGTAGAAATAC+AGG + chr8_1:80722936-80722955 None:intergenic 40.0%
TTGTAATGTGTGTGCCATGA+TGG + chr8_1:80723044-80723063 None:intergenic 40.0%
CTAGACTATGGTACATGCAT+TGG + chr8_1:80723141-80723160 None:intergenic 40.0%
GTATAGTGCCTTAGACATAG+AGG - chr8_1:80723160-80723179 Msa1218150:CDS 40.0%
TGGATCAGCCACTCTTACAT+GGG + chr8_1:80722312-80722331 None:intergenic 45.0%
ATGGATCAGCCACTCTTACA+TGG + chr8_1:80722313-80722332 None:intergenic 45.0%
ACTTGTATCGTTGTGACTCG+AGG + chr8_1:80722348-80722367 None:intergenic 45.0%
CACTCAATGGCATCAATGGT+AGG - chr8_1:80722409-80722428 Msa1218150:three_prime_UTR 45.0%
!! TCGTTGACTTCGATCGACAA+AGG - chr8_1:80722529-80722548 Msa1218150:three_prime_UTR 45.0%
AACCTCCTTTCCAGAACCAA+TGG - chr8_1:80722725-80722744 Msa1218150:CDS 45.0%
!! AACCATTGGTTCTGGAAAGG+AGG + chr8_1:80722730-80722749 None:intergenic 45.0%
!! CTCAACCATTGGTTCTGGAA+AGG + chr8_1:80722733-80722752 None:intergenic 45.0%
CTTTCCAGAACCAATGGTTG+AGG - chr8_1:80722731-80722750 Msa1218150:CDS 45.0%
!! CATGTAGGTGTTGGTGATGA+TGG + chr8_1:80722762-80722781 None:intergenic 45.0%
CATCATCACCAACACCTACA+TGG - chr8_1:80722760-80722779 Msa1218150:CDS 45.0%
TGTCTTGCATTGTGCCATGT+AGG + chr8_1:80722777-80722796 None:intergenic 45.0%
AGGAACTGAAGATGCAGAAG+AGG - chr8_1:80722815-80722834 Msa1218150:CDS 45.0%
TAACATGGTTAGGCCGATGA+TGG + chr8_1:80722854-80722873 None:intergenic 45.0%
GTCTCTCTCCTCTATGTCTA+AGG + chr8_1:80723171-80723190 None:intergenic 45.0%
CGCAAAACAGAAACATGCTC+AGG - chr8_1:80723420-80723439 Msa1218150:five_prime_UTR 45.0%
TGGATCAGCCACTCTTACAT+GGG + chr8_1:80722312-80722331 None:intergenic 45.0%
ATGGATCAGCCACTCTTACA+TGG + chr8_1:80722313-80722332 None:intergenic 45.0%
ACTTGTATCGTTGTGACTCG+AGG + chr8_1:80722348-80722367 None:intergenic 45.0%
CACTCAATGGCATCAATGGT+AGG - chr8_1:80722409-80722428 Msa1218150:three_prime_UTR 45.0%
!! TCGTTGACTTCGATCGACAA+AGG - chr8_1:80722529-80722548 Msa1218150:three_prime_UTR 45.0%
AACCTCCTTTCCAGAACCAA+TGG - chr8_1:80722725-80722744 Msa1218150:CDS 45.0%
!! AACCATTGGTTCTGGAAAGG+AGG + chr8_1:80722730-80722749 None:intergenic 45.0%
!! CTCAACCATTGGTTCTGGAA+AGG + chr8_1:80722733-80722752 None:intergenic 45.0%
CTTTCCAGAACCAATGGTTG+AGG - chr8_1:80722731-80722750 Msa1218150:CDS 45.0%
!! CATGTAGGTGTTGGTGATGA+TGG + chr8_1:80722762-80722781 None:intergenic 45.0%
CATCATCACCAACACCTACA+TGG - chr8_1:80722760-80722779 Msa1218150:CDS 45.0%
TGTCTTGCATTGTGCCATGT+AGG + chr8_1:80722777-80722796 None:intergenic 45.0%
AGGAACTGAAGATGCAGAAG+AGG - chr8_1:80722815-80722834 Msa1218150:CDS 45.0%
TAACATGGTTAGGCCGATGA+TGG + chr8_1:80722854-80722873 None:intergenic 45.0%
GTCTCTCTCCTCTATGTCTA+AGG + chr8_1:80723171-80723190 None:intergenic 45.0%
CGCAAAACAGAAACATGCTC+AGG - chr8_1:80723420-80723439 Msa1218150:five_prime_UTR 45.0%
GCAAGCATCCCATGTAAGAG+TGG - chr8_1:80722301-80722320 Msa1218150:three_prime_UTR 50.0%
! CTCGAGGTGTCTGCATTTGA+TGG + chr8_1:80722332-80722351 None:intergenic 50.0%
CTTCAAGCTCCACAAGCTCA+AGG - chr8_1:80722702-80722721 Msa1218150:CDS 50.0%
AAGGAGGTTCCTTGAGCTTG+TGG + chr8_1:80722714-80722733 None:intergenic 50.0%
!! GTTGCCTCAACCATTGGTTC+TGG + chr8_1:80722738-80722757 None:intergenic 50.0%
!! GATGGTGTTGCCTCAACCAT+TGG + chr8_1:80722744-80722763 None:intergenic 50.0%
GCATTGTGCCATGTAGGTGT+TGG + chr8_1:80722771-80722790 None:intergenic 50.0%
CAGCAGTGGCAGAACAATCT+AGG - chr8_1:80722795-80722814 Msa1218150:CDS 50.0%
GGGATTGTCATCGCTGAACA+TGG + chr8_1:80723120-80723139 None:intergenic 50.0%
GCAAGCATCCCATGTAAGAG+TGG - chr8_1:80722301-80722320 Msa1218150:three_prime_UTR 50.0%
! CTCGAGGTGTCTGCATTTGA+TGG + chr8_1:80722332-80722351 None:intergenic 50.0%
CTTCAAGCTCCACAAGCTCA+AGG - chr8_1:80722702-80722721 Msa1218150:CDS 50.0%
AAGGAGGTTCCTTGAGCTTG+TGG + chr8_1:80722714-80722733 None:intergenic 50.0%
!! GTTGCCTCAACCATTGGTTC+TGG + chr8_1:80722738-80722757 None:intergenic 50.0%
!! GATGGTGTTGCCTCAACCAT+TGG + chr8_1:80722744-80722763 None:intergenic 50.0%
GCATTGTGCCATGTAGGTGT+TGG + chr8_1:80722771-80722790 None:intergenic 50.0%
CAGCAGTGGCAGAACAATCT+AGG - chr8_1:80722795-80722814 Msa1218150:CDS 50.0%
GGGATTGTCATCGCTGAACA+TGG + chr8_1:80723120-80723139 None:intergenic 50.0%
GGCACAATGCAAGACAGCAG+TGG - chr8_1:80722781-80722800 Msa1218150:CDS 55.0%
GGCACAATGCAAGACAGCAG+TGG - chr8_1:80722781-80722800 Msa1218150:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 80722249 80723501 80722249 ID=Msa1218150;Name=Msa1218150
chr8_1 mRNA 80722249 80723501 80722249 ID=Msa1218150-mRNA-1;Parent=Msa1218150;Name=Msa1218150-mRNA-1;_AED=0.03;_eAED=0.03;_QI=87|1|1|1|1|1|2|341|245
chr8_1 exon 80722249 80723236 80722249 ID=Msa1218150-mRNA-1:exon:28389;Parent=Msa1218150-mRNA-1
chr8_1 exon 80723324 80723501 80723324 ID=Msa1218150-mRNA-1:exon:28388;Parent=Msa1218150-mRNA-1
chr8_1 five_prime_UTR 80723415 80723501 80723415 ID=Msa1218150-mRNA-1:five_prime_utr;Parent=Msa1218150-mRNA-1
chr8_1 CDS 80723324 80723414 80723324 ID=Msa1218150-mRNA-1:cds;Parent=Msa1218150-mRNA-1
chr8_1 CDS 80722590 80723236 80722590 ID=Msa1218150-mRNA-1:cds;Parent=Msa1218150-mRNA-1
chr8_1 three_prime_UTR 80722249 80722589 80722249 ID=Msa1218150-mRNA-1:three_prime_utr;Parent=Msa1218150-mRNA-1
Gene Sequence

>Msa1218150

ATGCAGACACCTCGAGTCACAACGATACAAGTCCGAGTTGATTGTAACGGATGTGCGCAGAAGATCAAGAAAGCACTCAATGGCATCAATGGTATACATGATCTTCTCGTTGACTTCGATCGACAAAGGCTAACAGTTATTGGATGGGCAGATCCAGAAAATATTGTCAAAGCAATTAAGAAGAAGGCAAAGAAGAATGCCACCATTATTTGTTCCAATATTGAACCTACAACTTCATCTAAAGATTCTAAACCAACAAAACCAAAACCAAAAGAAAATCTTCAAGCTCCACAAGCTCAAGGAACCTCCTTTCCAGAACCAATGGTTGAGGCAACACCATCATCACCAACACCTACATGGCACAATGCAAGACAGCAGTGGCAGAACAATCTAGGAACTGAAGATGCAGAAGAGGCTCATATGTTACACCATCATCGGCCTAACCATGTTAACAGAATTGGTTCTGGGAATAACTATGTTGACCATAGAAACAGATTATACCATAATGACCCTGTATTTCTACAAGAGCCATCTAAAATCCAACCATTGAATGTCATACATAGTTACAATACATACATGCCATCATCTCATATCACTGAATATGAATGTGTTAGATCACCATCATGGCACACACATTACAACTATATAGAACACTACAGTGGAGACTACAATGACAACAATGTAGATATAGCAGCCATGTTCAGCGATGACAATCCCAATGCATGTACCATAGTCTAG
Protein sequence

>Msa1218150

MQTPRVTTIQVRVDCNGCAQKIKKALNGINGIHDLLVDFDRQRLTVIGWADPENIVKAIKKKAKKNATIICSNIEPTTSSKDSKPTKPKPKENLQAPQAQGTSFPEPMVEATPSSPTPTWHNARQQWQNNLGTEDAEEAHMLHHHRPNHVNRIGSGNNYVDHRNRLYHNDPVFLQEPSKIQPLNVIHSYNTYMPSSHITEYECVRSPSWHTHYNYIEHYSGDYNDNNVDIAAMFSDDNPNACTIV*