AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1360930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1360930 MtrunA17_Chr3g0130741 97.315 447 10 1 1 447 1 445 0.0 889
Msa1360930 MtrunA17_Chr8g0384731 72.158 431 102 4 18 447 22 435 0.0 647
Msa1360930 MtrunA17_Chr1g0159791 55.556 378 162 5 68 441 39 414 7.07e-142 412
Msa1360930 MtrunA17_Chr4g0010951 45.232 409 184 9 71 442 46 451 2.09e-114 343
Msa1360930 MtrunA17_Chr6g0484731 44.855 379 192 11 71 436 176 550 1.13e-95 299
Msa1360930 MtrunA17_Chr3g0078321 37.000 400 223 14 55 440 32 416 4.23e-75 241
Msa1360930 MtrunA17_Chr4g0010941 44.492 236 99 4 235 440 2 235 9.77e-64 205
Msa1360930 MtrunA17_Chr1g0179751 25.783 415 256 13 66 444 84 482 2.29e-34 134
Msa1360930 MtrunA17_Chr1g0189381 26.984 441 267 17 40 446 20 439 1.87e-33 131
Msa1360930 MtrunA17_Chr7g0249451 26.585 410 250 17 66 444 49 438 7.15e-32 127
Msa1360930 MtrunA17_Chr7g0265331 26.214 412 238 17 75 444 68 455 5.31e-31 124
Msa1360930 MtrunA17_Chr2g0320621 26.866 402 248 16 66 437 53 438 2.01e-29 120
Msa1360930 MtrunA17_Chr7g0221681 27.419 434 256 19 47 444 31 441 7.18e-29 118
Msa1360930 MtrunA17_Chr4g0027681 25.063 395 247 15 73 437 62 437 1.13e-28 118
Msa1360930 MtrunA17_Chr4g0053721 27.007 411 251 17 52 436 43 430 3.04e-28 117
Msa1360930 MtrunA17_Chr5g0425141 28.075 374 211 17 91 436 106 449 1.31e-26 112
Msa1360930 MtrunA17_Chr8g0352671 25.000 400 247 14 65 439 68 439 7.71e-26 109
Msa1360930 MtrunA17_Chr5g0435871 25.500 400 243 16 65 439 68 437 1.38e-25 108
Msa1360930 MtrunA17_Chr5g0401601 26.400 375 236 15 85 439 87 441 1.55e-25 109
Msa1360930 MtrunA17_Chr5g0406001 27.174 368 223 12 91 436 117 461 4.00e-25 107
Msa1360930 MtrunA17_Chr8g0386441 25.000 396 248 14 65 439 65 432 4.09e-25 107
Msa1360930 MtrunA17_Chr5g0435971 25.500 400 243 16 65 439 65 434 6.59e-25 106
Msa1360930 MtrunA17_Chr8g0371371 29.288 379 217 19 91 443 117 470 7.70e-25 107
Msa1360930 MtrunA17_Chr2g0310831 25.445 393 243 16 66 438 80 442 1.14e-23 102
Msa1360930 MtrunA17_Chr8g0352661 25.320 391 240 15 72 439 78 439 2.01e-23 102
Msa1360930 MtrunA17_Chr3g0130931 25.564 399 228 22 71 439 92 451 4.07e-23 101
Msa1360930 MtrunA17_Chr5g0395911 26.357 387 224 16 80 438 62 415 2.89e-22 98.6
Msa1360930 MtrunA17_Chr2g0333991 25.984 381 237 15 76 435 78 434 3.36e-22 98.6
Msa1360930 MtrunA17_Chr3g0124421 25.831 391 223 15 91 447 104 461 4.17e-22 99.0
Msa1360930 MtrunA17_Chr2g0311451 25.260 384 242 15 76 438 78 437 1.51e-21 96.7
Msa1360930 MtrunA17_Chr2g0310801 24.485 388 246 15 68 438 78 435 9.59e-21 94.4
Msa1360930 MtrunA17_Chr3g0087851 24.607 382 237 16 82 439 85 439 8.44e-20 91.3
Msa1360930 MtrunA17_Chr8g0371351 26.455 378 228 16 84 436 97 449 2.81e-19 90.1
Msa1360930 MtrunA17_Chr7g0275731 28.117 377 216 19 85 437 140 485 1.15e-18 88.2
Msa1360930 MtrunA17_Chr2g0310821 23.117 385 248 15 68 439 96 445 1.92e-17 84.3
Msa1360930 MtrunA17_Chr4g0001991 26.005 373 231 19 88 439 172 520 1.42e-16 82.0
Msa1360930 MtrunA17_Chr5g0442921 26.067 445 258 18 34 439 144 556 1.79e-16 81.6
Msa1360930 MtrunA17_Chr2g0308001 24.096 415 247 17 68 446 36 418 1.84e-16 81.3
Msa1360930 MtrunA17_Chr6g0487471 27.368 285 169 10 85 343 85 357 1.12e-15 79.0
Msa1360930 MtrunA17_Chr5g0434411 23.136 389 233 12 89 441 84 442 2.23e-15 77.8
Msa1360930 MtrunA17_Chr5g0395871 25.000 376 227 18 85 436 80 424 4.51e-15 77.0
Msa1360930 MtrunA17_Chr8g0390761 30.357 168 98 6 89 252 119 271 5.00e-15 77.0
Msa1360930 MtrunA17_Chr1g0212321 31.757 148 85 6 85 228 145 280 1.74e-14 75.5
Msa1360930 MtrunA17_Chr2g0291201 23.936 376 239 15 85 437 101 452 3.49e-14 74.3
Msa1360930 MtrunA17_Chr6g0484901 24.242 363 226 16 91 436 161 491 3.93e-14 74.3
Msa1360930 MtrunA17_Chr1g0179091 23.058 399 227 17 64 438 26 368 4.89e-14 73.2
Msa1360930 MtrunA17_Chr3g0099901 24.267 375 245 14 79 437 135 486 9.39e-14 73.2
Msa1360930 MtrunA17_Chr3g0095891 23.620 453 266 20 31 439 45 461 2.88e-13 71.6
Msa1360930 MtrunA17_Chr3g0099891 25.000 376 237 17 77 436 108 454 9.01e-13 70.1
Msa1360930 MtrunA17_Chr1g0210201 25.000 372 225 19 85 436 130 467 1.73e-12 68.9
Msa1360930 MtrunA17_Chr5g0434441 23.316 386 238 12 89 441 71 431 2.46e-11 65.5
Msa1360930 MtrunA17_Chr7g0239001 22.646 393 236 18 89 439 62 428 6.33e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1360930 AT4G33490.2 65.206 388 132 3 63 447 35 422 0.0 536
Msa1360930 AT4G33490.1 67.330 352 112 3 63 411 32 383 2.64e-175 497
Msa1360930 AT1G44130.1 51.724 377 177 5 69 442 31 405 1.33e-142 414
Msa1360930 AT1G77480.2 46.867 399 207 5 49 443 29 426 1.80e-131 386
Msa1360930 AT1G77480.1 46.717 396 206 5 49 440 29 423 1.22e-129 383
Msa1360930 AT1G49050.1 44.836 397 194 14 71 447 187 578 6.89e-94 295
Msa1360930 AT1G49050.2 43.570 381 192 13 86 447 29 405 1.15e-87 273
Msa1360930 AT1G49050.3 43.911 271 134 9 71 325 187 455 1.41e-58 199
Msa1360930 AT1G65240.1 28.960 404 238 14 68 444 53 434 3.18e-38 145
Msa1360930 AT2G36670.2 26.521 411 243 15 70 447 81 465 8.28e-34 133
Msa1360930 AT5G22850.1 26.316 399 244 15 70 437 62 441 9.85e-34 132
Msa1360930 AT3G02740.1 29.250 400 238 16 71 444 67 447 1.10e-32 129
Msa1360930 AT5G43100.2 28.841 371 224 15 85 439 74 420 1.59e-32 129
Msa1360930 AT5G36260.1 27.273 385 238 12 84 444 75 441 3.05e-32 128
Msa1360930 AT5G43100.1 28.841 371 224 15 85 439 74 420 8.01e-32 128
Msa1360930 AT2G36670.1 26.202 416 243 16 70 447 81 470 1.12e-31 127
Msa1360930 AT3G02740.2 29.124 388 232 15 81 444 4 372 2.71e-31 124
Msa1360930 AT2G36670.4 25.758 396 231 14 86 447 33 399 2.44e-30 122
Msa1360930 AT2G36670.3 25.758 396 231 14 86 447 33 399 2.44e-30 122
Msa1360930 AT1G05840.1 26.904 394 229 13 85 444 78 446 2.84e-30 122
Msa1360930 AT1G08210.1 26.931 479 280 18 7 445 9 457 1.15e-28 118
Msa1360930 AT1G08210.4 26.931 479 280 18 7 445 43 491 1.53e-28 118
Msa1360930 AT1G08210.3 26.931 479 280 18 7 445 57 505 2.07e-28 118
Msa1360930 AT3G50050.1 26.781 407 260 14 51 439 53 439 4.11e-28 117
Msa1360930 AT2G17760.1 29.973 377 219 19 81 436 97 449 2.18e-27 114
Msa1360930 AT1G08210.2 25.845 503 282 19 7 445 9 484 3.82e-24 105
Msa1360930 AT5G10080.1 26.984 378 220 14 91 438 104 455 8.61e-23 101
Msa1360930 AT3G25700.1 26.702 382 236 17 85 436 82 449 3.85e-22 99.0
Msa1360930 AT4G35880.1 26.702 382 230 15 84 442 103 457 1.31e-21 97.8
Msa1360930 AT5G10770.1 26.203 374 238 15 79 437 124 474 1.53e-20 94.4
Msa1360930 AT3G51330.3 26.415 371 242 15 84 437 98 454 1.82e-20 94.0
Msa1360930 AT3G51330.1 26.486 370 241 15 84 436 98 453 2.15e-20 94.0
Msa1360930 AT2G28010.1 32.000 200 113 7 58 256 39 216 5.46e-19 89.0
Msa1360930 AT3G51360.7 24.462 372 242 12 84 437 2 352 6.81e-19 88.6
Msa1360930 AT3G51360.9 24.932 365 235 12 91 437 103 446 1.06e-18 89.0
Msa1360930 AT3G51360.1 24.932 365 235 12 91 437 93 436 1.12e-18 88.6
Msa1360930 AT1G66180.1 22.921 445 261 18 27 439 35 429 6.38e-17 83.2
Msa1360930 AT4G35880.2 26.163 344 209 14 84 404 103 424 5.40e-16 80.1
Msa1360930 AT3G51340.2 26.216 370 235 14 84 436 99 447 8.04e-16 80.1
Msa1360930 AT1G01300.1 26.260 377 221 18 85 436 140 484 1.26e-15 79.3
Msa1360930 AT4G12920.1 25.490 255 172 10 87 334 58 301 1.54e-15 78.6
Msa1360930 AT1G64830.1 28.094 299 179 15 85 360 84 369 4.37e-15 77.4
Msa1360930 AT3G51360.2 28.279 244 152 7 91 319 103 338 6.98e-15 76.3
Msa1360930 AT3G18490.1 24.935 385 228 21 77 436 152 500 9.57e-15 76.6
Msa1360930 AT3G51360.5 28.689 244 151 8 91 319 103 338 1.23e-14 75.9
Msa1360930 AT3G51360.6 28.689 244 151 8 91 319 103 338 1.25e-14 76.3
Msa1360930 AT3G51360.4 28.689 244 151 8 91 319 93 328 2.13e-14 75.1
Msa1360930 AT3G51360.10 28.689 244 151 8 91 319 93 328 2.35e-14 75.1
Msa1360930 AT3G51340.1 25.526 380 237 15 84 436 99 459 3.09e-14 75.1
Msa1360930 AT5G37540.1 23.641 423 265 16 52 440 44 442 7.82e-14 73.6
Msa1360930 AT4G30030.1 24.668 377 235 18 83 439 75 422 2.64e-13 72.0
Msa1360930 AT2G28040.2 24.228 421 252 19 34 437 12 382 3.63e-13 71.2
Msa1360930 AT2G23945.1 27.338 278 168 11 89 346 98 361 8.86e-13 70.5
Msa1360930 AT2G28040.1 24.060 399 235 19 66 439 38 393 1.88e-12 68.9
Msa1360930 AT5G10760.1 27.519 258 154 10 85 326 130 370 1.90e-12 69.3
Msa1360930 AT1G25510.1 23.360 381 236 16 77 436 138 483 4.02e-12 68.6
Msa1360930 AT3G20015.1 29.924 264 152 11 85 329 129 378 4.22e-12 68.2
Msa1360930 AT5G02190.1 23.980 392 218 23 96 437 82 443 1.34e-11 66.6
Msa1360930 AT2G35615.1 21.851 389 241 14 85 437 83 444 3.18e-11 65.5
Msa1360930 AT1G31450.1 23.773 387 234 16 85 437 83 442 6.68e-11 64.7
Msa1360930 AT3G25700.2 32.812 128 75 6 85 202 82 208 8.61e-11 63.5

Find 130 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTGACATCTTTGGATTT+TGG 0.149011 tig0017277:-31506 None:intergenic
TAATCAAATGCTGACATCTT+TGG 0.242452 tig0017277:-31513 None:intergenic
CTTATCAAGCACTAATTTCT+TGG 0.254030 tig0017277:+30640 Msa1360930:CDS
TGGTTCATCGGTTGTGTTCC+CGG 0.283511 tig0017277:+28309 Msa1360930:CDS
ACTTTATAGAACACGATATA+TGG 0.292279 tig0017277:+31604 Msa1360930:three_prime_UTR
AATCATGAAGTCAGGGAAAT+TGG 0.293581 tig0017277:+28099 Msa1360930:exon
GCTTTCGATCTTCCACTTCC+TGG 0.304667 tig0017277:-31008 None:intergenic
GGCGGAGGATACATCTTCTT+TGG 0.305148 tig0017277:+30365 Msa1360930:CDS
AGAGGAAGAGTTTGGTCTTC+AGG 0.323198 tig0017277:-30909 None:intergenic
AGACACCACATCCGCTTTAC+CGG 0.336790 tig0017277:+29362 Msa1360930:intron
GTGTCAATATCAAGAAAATA+TGG 0.341260 tig0017277:-29101 None:intergenic
GACAATGAAAAGCAGTTGAT+AGG 0.341276 tig0017277:+31455 Msa1360930:CDS
CTTCAGGAGGGATTTCGAAT+TGG 0.346518 tig0017277:-31031 None:intergenic
TCGGTTGTGTTCCCGGTTCA+TGG 0.346643 tig0017277:+28316 Msa1360930:CDS
TTCTTGGAGGGAAGAGGACT+GGG 0.346941 tig0017277:+30556 Msa1360930:CDS
CGCCATTCCTACCTCCGATT+AGG 0.353194 tig0017277:-31254 None:intergenic
TAGTTTGATTGCACGGCATT+GGG 0.360844 tig0017277:+31775 Msa1360930:three_prime_UTR
TTGGGGATTTCATTTAGAAT+TGG 0.362523 tig0017277:+31793 Msa1360930:three_prime_UTR
GGAGGGAAGAGGACTGGGTT+TGG 0.363106 tig0017277:+30561 Msa1360930:CDS
TCTACTTGTTTAGCTTGGTT+TGG 0.365632 tig0017277:+28154 Msa1360930:CDS
TCTTCCTACCACCTCGTAGA+TGG 0.372260 tig0017277:+30245 Msa1360930:CDS
ACTTCATATACACTTCTAAA+AGG 0.375337 tig0017277:-30951 None:intergenic
TTCAGGAGGGATTTCGAATT+GGG 0.375689 tig0017277:-31030 None:intergenic
GGATAAACATTTCCATGAAC+CGG 0.391556 tig0017277:-28328 None:intergenic
TAACATGATATGTTGTTATA+TGG 0.393852 tig0017277:-31754 None:intergenic
CCTGGGAAGCTAAGTGCAAT+AGG 0.403734 tig0017277:-30990 None:intergenic
GCCTTGTTCGCGTTGCTCTC+AGG 0.405030 tig0017277:+29157 Msa1360930:CDS
TTATCAAGCACTAATTTCTT+GGG 0.408330 tig0017277:+30641 Msa1360930:CDS
GATATAATCAGATATGCTTC+AGG 0.410114 tig0017277:-31047 None:intergenic
ATGGAATGCTTGGCCTTGGC+AGG 0.412501 tig0017277:+30264 Msa1360930:CDS
CTCAGCAAACATTACTCAGC+AGG 0.412530 tig0017277:+30519 Msa1360930:intron
CATGGAAATGTTTATCCTGT+TGG 0.413700 tig0017277:+28334 Msa1360930:CDS
ACGAGGTGGTAGGAAGAATC+TGG 0.415099 tig0017277:-30239 None:intergenic
ATGGAAATGTTTATCCTGTT+GGG 0.418408 tig0017277:+28335 Msa1360930:CDS
GTTCCTCTACTTGTTTAGCT+TGG 0.418515 tig0017277:+28149 Msa1360930:CDS
CATCACATTGAAGCCATGTT+AGG 0.420882 tig0017277:-29133 None:intergenic
CTTAAAGTTCGTATGGCACT+TGG 0.422527 tig0017277:+29565 Msa1360930:CDS
AATAGAGAAATCATGAAGTC+AGG 0.426807 tig0017277:+28091 Msa1360930:exon
ATTTCAAGTTCAAGTGCAAA+TGG 0.428003 tig0017277:+28205 Msa1360930:CDS
CTTTCGATCTTCCACTTCCT+GGG 0.434979 tig0017277:-31007 None:intergenic
TATGTTCTCAACTTTACAAA+TGG 0.435278 tig0017277:+29535 Msa1360930:CDS
TGTTTATGATTCTTCTCGAT+TGG 0.447111 tig0017277:+30391 Msa1360930:CDS
GTTCTTGGAGGGAAGAGGAC+TGG 0.447212 tig0017277:+30555 Msa1360930:CDS
ACCAACATAGAGGAAGAGTT+TGG 0.449229 tig0017277:-30917 None:intergenic
TTAGTTTGATTGCACGGCAT+TGG 0.449305 tig0017277:+31774 Msa1360930:three_prime_UTR
GATATCACAGCTGAATGGTC+AGG 0.452709 tig0017277:+30301 Msa1360930:CDS
ATGATTCTTCTCGATTGGCT+TGG 0.455289 tig0017277:+30396 Msa1360930:CDS
CCTATTGCACTTAGCTTCCC+AGG 0.459180 tig0017277:+30990 Msa1360930:CDS
ATAATGTGACTATCAACATT+GGG 0.460427 tig0017277:+29066 Msa1360930:CDS
AACATGGGAAATGTTTGCTT+GGG 0.462552 tig0017277:+31192 Msa1360930:CDS
GTGCATAGTATATTGTTAAG+TGG 0.465634 tig0017277:+31668 Msa1360930:three_prime_UTR
ATATCAAGAAAATATGGCCT+TGG 0.468926 tig0017277:-29095 None:intergenic
GCACTTAGCTTCCCAGGAAG+TGG 0.475231 tig0017277:+30996 Msa1360930:CDS
GTAGATGGAATGCTTGGCCT+TGG 0.477499 tig0017277:+30260 Msa1360930:CDS
GCACAGAGGGGATGCCTGCA+TGG 0.478162 tig0017277:-29403 None:intergenic
GAACATGGGAAATGTTTGCT+TGG 0.481022 tig0017277:+31191 Msa1360930:intron
GTGGAAGATGTAAACCTAAT+CGG 0.481109 tig0017277:+31240 Msa1360930:CDS
ACTCTTCCTCTATGTTGGTA+TGG 0.484010 tig0017277:+30921 Msa1360930:CDS
ATATATCGTGTTCTATAAAG+TGG 0.488423 tig0017277:-31602 None:intergenic
ATTCTAGACGGCTCTGAAGT+AGG 0.488461 tig0017277:+31216 Msa1360930:CDS
TGGCTGATCACTTTGTCAGT+TGG 0.491728 tig0017277:-31734 None:intergenic
TCAAGAAAATATGGCCTTGG+TGG 0.495645 tig0017277:-29092 None:intergenic
CACCTCGTAGATGGAATGCT+TGG 0.499828 tig0017277:+30254 Msa1360930:CDS
ACGATATATGGTTGTATGAA+AGG 0.500199 tig0017277:+31616 Msa1360930:three_prime_UTR
ATCATGTTAGTTTGATTGCA+CGG 0.504799 tig0017277:+31768 Msa1360930:three_prime_UTR
TACCATCACTTTGTCTAGCA+TGG 0.505530 tig0017277:-31429 None:intergenic
CGAAAACGATTCAAGAATGA+AGG 0.508091 tig0017277:-28283 None:intergenic
GTGCCATACGAACTTTAAGT+TGG 0.511112 tig0017277:-29561 None:intergenic
GCAGATCATTACTCATCTCT+TGG 0.518717 tig0017277:+29493 Msa1360930:CDS
TATAATGTGACTATCAACAT+TGG 0.520628 tig0017277:+29065 Msa1360930:CDS
CTAGGCCGGTAAAGCGGATG+TGG 0.520658 tig0017277:-29367 None:intergenic
AGATAATCTAAGAGTAATGC+AGG 0.524455 tig0017277:+31826 Msa1360930:three_prime_UTR
TATTGTTAAGTGGCATATAG+AGG 0.527766 tig0017277:+31678 Msa1360930:three_prime_UTR
ACAATGAAAAGCAGTTGATA+GGG 0.527865 tig0017277:+31456 Msa1360930:CDS
GTGATGCACGGATGCACAGA+GGG 0.529685 tig0017277:-29416 None:intergenic
GGAGCTGAACTTGTTCTTGG+AGG 0.531520 tig0017277:+30543 Msa1360930:CDS
GATGACGACGACGACGATGA+AGG 0.535925 tig0017277:-28256 None:intergenic
CATGGGACTAAGTCATTGCT+AGG 0.535975 tig0017277:-29385 None:intergenic
ACCAAACTCTTCCTCTATGT+TGG 0.539118 tig0017277:+30916 Msa1360930:CDS
AACTTGTTCTTGGAGGGAAG+AGG 0.539126 tig0017277:+30550 Msa1360930:CDS
TGGAATGCTTGGCCTTGGCA+GGG 0.541186 tig0017277:+30265 Msa1360930:CDS
GGAATGCTTGGCCTTGGCAG+GGG 0.545359 tig0017277:+30266 Msa1360930:CDS
GAGCTGAACTTGTTCTTGGA+GGG 0.545394 tig0017277:+30544 Msa1360930:CDS
TACTAATCTCTCGACGACAT+TGG 0.546672 tig0017277:-30422 None:intergenic
AGTTTGATTGCACGGCATTG+GGG 0.551103 tig0017277:+31776 Msa1360930:three_prime_UTR
ATATCACAGCTGAATGGTCA+GGG 0.557396 tig0017277:+30302 Msa1360930:CDS
CTGTGCATCCGTGCATCACA+CGG 0.557548 tig0017277:+29420 Msa1360930:CDS
TCAGCAAACATTACTCAGCA+GGG 0.557841 tig0017277:+30520 Msa1360930:intron
ATAGAGAAATCATGAAGTCA+GGG 0.558907 tig0017277:+28092 Msa1360930:exon
AGGTTGTTCATATGAAAGAA+TGG 0.559496 tig0017277:-28236 None:intergenic
CACAGAGGGGATGCCTGCAT+GGG 0.564456 tig0017277:-29402 None:intergenic
GGACTAAGTCATTGCTAGGC+CGG 0.569566 tig0017277:-29381 None:intergenic
GTTTGCTTGGGCATTCTAGA+CGG 0.569919 tig0017277:+31204 Msa1360930:CDS
GTAGTTATCCGTGTGATGCA+CGG 0.570573 tig0017277:-29428 None:intergenic
TTAAAGTTCGTATGGCACTT+GGG 0.575859 tig0017277:+29566 Msa1360930:CDS
ACACGGGTAGCGACCTAACA+TGG 0.578697 tig0017277:+29120 Msa1360930:CDS
ACCTGAGAGCAACGCGAACA+AGG 0.580548 tig0017277:-29158 None:intergenic
GGTTTAGTGAGAAATGTCGT+TGG 0.585900 tig0017277:+30323 Msa1360930:CDS
GCAATGACTTAGTCCCATGC+AGG 0.592637 tig0017277:+29389 Msa1360930:CDS
CAAGAAAATATGGCCTTGGT+GGG 0.593840 tig0017277:-29091 None:intergenic
TTTCCATATGTAGTTGACGA+AGG 0.600659 tig0017277:+30857 Msa1360930:intron
AGCTTGATATCACAGCTGAA+TGG 0.601370 tig0017277:+30296 Msa1360930:CDS
TGTTTGAGTGCACAAGGCGG+AGG 0.601478 tig0017277:+30350 Msa1360930:CDS
AGTCCAACTTAAAGTTCGTA+TGG 0.601835 tig0017277:+29558 Msa1360930:CDS
ATTCAGCTGTGATATCAAGC+TGG 0.604358 tig0017277:-30294 None:intergenic
AGACGGCTCTGAAGTAGGAG+TGG 0.605898 tig0017277:+31221 Msa1360930:CDS
AAACCAAGCTAAACAAGTAG+AGG 0.606468 tig0017277:-28152 None:intergenic
TAGTTGACGAAGGAATTAGC+TGG 0.606843 tig0017277:+30867 Msa1360930:intron
AAATTGTTCTTCCAGAACAT+GGG 0.610504 tig0017277:+31177 Msa1360930:intron
CATTCCATCTACGAGGTGGT+AGG 0.611904 tig0017277:-30249 None:intergenic
TATATCGTGTTCTATAAAGT+GGG 0.612282 tig0017277:-31601 None:intergenic
ATAATCAGATATGCTTCAGG+AGG 0.624631 tig0017277:-31044 None:intergenic
GGACACTGTTTGAGTGCACA+AGG 0.625207 tig0017277:+30344 Msa1360930:CDS
TTTGATTACGAAAGATACAT+CGG 0.632392 tig0017277:+31528 Msa1360930:exon
ATGAAAAGCAGTTGATAGGG+TGG 0.632654 tig0017277:+31459 Msa1360930:CDS
ATCCATGCTAGACAAAGTGA+TGG 0.638414 tig0017277:+31427 Msa1360930:CDS
TCAACATTGGGTACCCACCA+AGG 0.642379 tig0017277:+29078 Msa1360930:CDS
GATAAACATTTCCATGAACC+GGG 0.645327 tig0017277:-28327 None:intergenic
AGAAAACTGAGACTATGTTG+TGG 0.653321 tig0017277:-28031 None:intergenic
AGCAAACATTACTCAGCAGG+GGG 0.654958 tig0017277:+30522 Msa1360930:intron
CAGCAAACATTACTCAGCAG+GGG 0.656453 tig0017277:+30521 Msa1360930:intron
TCATTGCTAGGCCGGTAAAG+CGG 0.656612 tig0017277:-29373 None:intergenic
TAATCAGATATGCTTCAGGA+GGG 0.677945 tig0017277:-31043 None:intergenic
ATGTAAACTGATTAACTGAG+TGG 0.681726 tig0017277:-31551 None:intergenic
GAAGATGTAAACCTAATCGG+AGG 0.690670 tig0017277:+31243 Msa1360930:CDS
CAAGCATTCCATCTACGAGG+TGG 0.700753 tig0017277:-30253 None:intergenic
TGTGATGCACGGATGCACAG+AGG 0.715403 tig0017277:-29417 None:intergenic
CACTGTTTGAGTGCACAAGG+CGG 0.732542 tig0017277:+30347 Msa1360930:CDS
GGCCAAGCATTCCATCTACG+AGG 0.742409 tig0017277:-30256 None:intergenic
TGATGCACGGATGCACAGAG+GGG 0.795188 tig0017277:-29415 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTATTCAAATTACTTT+AGG - tig0017277:28514-28533 None:intergenic 10.0%
!! AATAATAAAACATCTTTATA+AGG + tig0017277:28525-28544 Msa1360930:intron 10.0%
!!! CTTTTTTTTTAATATATAGA+TGG + tig0017277:28606-28625 Msa1360930:intron 10.0%
!! TAAATACTATTATTAGTTAA+TGG - tig0017277:29925-29944 None:intergenic 10.0%
!! AAAAAAAAAGCTTTCAATTT+GGG - tig0017277:28596-28615 None:intergenic 15.0%
!! TAAAAAAAAAGCTTTCAATT+TGG - tig0017277:28597-28616 None:intergenic 15.0%
!! TAAGTATAATTTATTCTGTT+TGG + tig0017277:28765-28784 Msa1360930:intron 15.0%
!!! TATTGAAGATTAATTTTGAT+AGG - tig0017277:29331-29350 None:intergenic 15.0%
!! AAAGTTTCCAATTTATATAT+GGG + tig0017277:29872-29891 Msa1360930:intron 15.0%
!! ATTATTAGTTAATGGTATTA+TGG - tig0017277:29917-29936 None:intergenic 15.0%
!!! TGATTTTTACTTCTTAATAA+AGG - tig0017277:30160-30179 None:intergenic 15.0%
!! TTTATTAAGAAGTAAAAATC+AGG + tig0017277:30159-30178 Msa1360930:intron 15.0%
!! AACAAAGAGAATGTAAAAAA+AGG - tig0017277:28377-28396 None:intergenic 20.0%
!!! TTCATTTTTTTTGTTTCCTT+CGG + tig0017277:28398-28417 Msa1360930:intron 20.0%
!! TAAAATAACAGAACAAAACA+TGG - tig0017277:28486-28505 None:intergenic 20.0%
!! ATTATTCAAATTACTTTAGG+AGG - tig0017277:28511-28530 None:intergenic 20.0%
!! ACAAAGAAAAACATGATTAT+GGG - tig0017277:28563-28582 None:intergenic 20.0%
!!! AATTTTGTCACCTTAAAAAA+TGG - tig0017277:28701-28720 None:intergenic 20.0%
!!! AAAATTGATTTTGACATGTT+TGG + tig0017277:28735-28754 Msa1360930:intron 20.0%
!! AATCAATTGTAAAAACCAAT+TGG - tig0017277:28929-28948 None:intergenic 20.0%
!!! CATTCAAAATCATTTTTTGT+TGG + tig0017277:28987-29006 Msa1360930:intron 20.0%
!!! ATATTTTCTTGATATTGACA+CGG + tig0017277:29103-29122 Msa1360930:CDS 20.0%
!! GAAAGTTTCCAATTTATATA+TGG + tig0017277:29871-29890 Msa1360930:intron 20.0%
!! AAGTTTCCAATTTATATATG+GGG + tig0017277:29873-29892 Msa1360930:intron 20.0%
!! TAAACTACTATTCTAAGTTT+GGG + tig0017277:29971-29990 Msa1360930:intron 20.0%
!! TTAATAAAGGATTAAGTGTA+TGG - tig0017277:30147-30166 None:intergenic 20.0%
!! AACTACATATGGAAATAAAA+CGG - tig0017277:30852-30871 None:intergenic 20.0%
!! AAAAGATAAAGCAAAAAAAG+TGG - tig0017277:31345-31364 None:intergenic 20.0%
!! TAACATGATATGTTGTTATA+TGG - tig0017277:31757-31776 None:intergenic 20.0%
! GACAAAGAAAAACATGATTA+TGG - tig0017277:28564-28583 None:intergenic 25.0%
!! AATCATATCAAGTGATTTTG+TGG + tig0017277:28643-28662 Msa1360930:intron 25.0%
! AACATACACATAAATTGGAA+CGG - tig0017277:28671-28690 None:intergenic 25.0%
! CCTATAACATACACATAAAT+TGG - tig0017277:28676-28695 None:intergenic 25.0%
! CCAATTTATGTGTATGTTAT+AGG + tig0017277:28673-28692 Msa1360930:intron 25.0%
!!! GTTATAGGTTCCATTTTTTA+AGG + tig0017277:28688-28707 Msa1360930:intron 25.0%
!!! AAGGTGACAAAATTGATTTT+AGG + tig0017277:28707-28726 Msa1360930:intron 25.0%
!!! AGGTGACAAAATTGATTTTA+GGG + tig0017277:28708-28727 Msa1360930:intron 25.0%
! TATAACATAAAGCTAGAAGT+TGG + tig0017277:28802-28821 Msa1360930:intron 25.0%
!! AATTTACCGCAATTTAAAAC+AGG - tig0017277:28830-28849 None:intergenic 25.0%
! GTAAAAGTGATTGAACAATA+AGG - tig0017277:28957-28976 None:intergenic 25.0%
!!! TCAAAATCATTTTTTGTTGG+CGG + tig0017277:28990-29009 Msa1360930:intron 25.0%
! TATAATGTGACTATCAACAT+TGG + tig0017277:29065-29084 Msa1360930:CDS 25.0%
! ATAATGTGACTATCAACATT+GGG + tig0017277:29066-29085 Msa1360930:CDS 25.0%
! GTGTCAATATCAAGAAAATA+TGG - tig0017277:29104-29123 None:intergenic 25.0%
!! TATTTTCTTGATATTGACAC+GGG + tig0017277:29104-29123 Msa1360930:CDS 25.0%
!!! TTCAAAGTTGTTAATTTGCT+AGG - tig0017277:29223-29242 None:intergenic 25.0%
! CTAATCTCATTTCAAGAAAA+AGG - tig0017277:29254-29273 None:intergenic 25.0%
! TATGTTCTCAACTTTACAAA+TGG + tig0017277:29535-29554 Msa1360930:CDS 25.0%
!!! TTTGTTTTCATAAACTATCC+CGG + tig0017277:29776-29795 Msa1360930:intron 25.0%
!!! AACTTTCACTTTAAAATCTC+CGG - tig0017277:29858-29877 None:intergenic 25.0%
! GTTTAGAAATAAGAACTTGA+TGG - tig0017277:29956-29975 None:intergenic 25.0%
! CTAAACTACTATTCTAAGTT+TGG + tig0017277:29970-29989 Msa1360930:intron 25.0%
! TTATCAAGCACTAATTTCTT+GGG + tig0017277:30641-30660 Msa1360930:CDS 25.0%
!! TTTAAAATGCTTGAATTTCG+AGG - tig0017277:30693-30712 None:intergenic 25.0%
! TCACTAATACGAGAAAATAT+TGG + tig0017277:30804-30823 Msa1360930:intron 25.0%
! ACTTCATATACACTTCTAAA+AGG - tig0017277:30954-30973 None:intergenic 25.0%
!! AATTGATAATATCCGTTAAC+AGG - tig0017277:31309-31328 None:intergenic 25.0%
! TTTGATTACGAAAGATACAT+CGG + tig0017277:31528-31547 Msa1360930:exon 25.0%
! TATATCGTGTTCTATAAAGT+GGG - tig0017277:31604-31623 None:intergenic 25.0%
! ATATATCGTGTTCTATAAAG+TGG - tig0017277:31605-31624 None:intergenic 25.0%
! ACTTTATAGAACACGATATA+TGG + tig0017277:31604-31623 Msa1360930:three_prime_UTR 25.0%
AATAGAGAAATCATGAAGTC+AGG + tig0017277:28091-28110 Msa1360930:exon 30.0%
ATAGAGAAATCATGAAGTCA+GGG + tig0017277:28092-28111 Msa1360930:exon 30.0%
!! ATTTCAAGTTCAAGTGCAAA+TGG + tig0017277:28205-28224 Msa1360930:CDS 30.0%
AGGTTGTTCATATGAAAGAA+TGG - tig0017277:28239-28258 None:intergenic 30.0%
ATGGAAATGTTTATCCTGTT+GGG + tig0017277:28335-28354 Msa1360930:CDS 30.0%
ATAACAGAACAAAACATGGA+GGG - tig0017277:28482-28501 None:intergenic 30.0%
AATAACAGAACAAAACATGG+AGG - tig0017277:28483-28502 None:intergenic 30.0%
AGAAAAACATGATTATGGGA+AGG - tig0017277:28559-28578 None:intergenic 30.0%
ACATACACATAAATTGGAAC+GGG - tig0017277:28670-28689 None:intergenic 30.0%
!! GGTGACAAAATTGATTTTAG+GGG + tig0017277:28709-28728 Msa1360930:intron 30.0%
CCTGCATATGTTAAAAGTAA+GGG - tig0017277:29042-29061 None:intergenic 30.0%
ACCTGCATATGTTAAAAGTA+AGG - tig0017277:29043-29062 None:intergenic 30.0%
ATATCAAGAAAATATGGCCT+TGG - tig0017277:29098-29117 None:intergenic 30.0%
! TGCTAGGTAATTTTGAATGT+TGG - tig0017277:29207-29226 None:intergenic 30.0%
CAGACATACATATATCTATC+TGG - tig0017277:29285-29304 None:intergenic 30.0%
!!! TTTAGGTTTTTAGTCCTAGT+TGG + tig0017277:29656-29675 Msa1360930:intron 30.0%
! CTTATTTCCATTATTTCTCC+GGG - tig0017277:29797-29816 None:intergenic 30.0%
! CCTTATTTCCATTATTTCTC+CGG - tig0017277:29798-29817 None:intergenic 30.0%
TGTAAGCCCCATATATAAAT+TGG - tig0017277:29882-29901 None:intergenic 30.0%
TTGAATCTATCTACTGACAT+AGG - tig0017277:30028-30047 None:intergenic 30.0%
AGAACATACACAGAGATATT+TGG - tig0017277:30061-30080 None:intergenic 30.0%
AAATATCTCTGTGTATGTTC+TGG + tig0017277:30060-30079 Msa1360930:intron 30.0%
!!! GCTATCTAGTACAACTTTTT+TGG + tig0017277:30181-30200 Msa1360930:intron 30.0%
!! TTGGTTATTTTATGCAGATG+CGG + tig0017277:30200-30219 Msa1360930:intron 30.0%
TGTTTATGATTCTTCTCGAT+TGG + tig0017277:30391-30410 Msa1360930:CDS 30.0%
CTTATCAAGCACTAATTTCT+TGG + tig0017277:30640-30659 Msa1360930:CDS 30.0%
GATATAATCAGATATGCTTC+AGG - tig0017277:31050-31069 None:intergenic 30.0%
! CTTGAAGAAAGTGATTAGTT+AGG - tig0017277:31085-31104 None:intergenic 30.0%
AAATTGTTCTTCCAGAACAT+GGG + tig0017277:31177-31196 Msa1360930:intron 30.0%
! ACAATGAAAAGCAGTTGATA+GGG + tig0017277:31456-31475 Msa1360930:CDS 30.0%
TAATCAAATGCTGACATCTT+TGG - tig0017277:31516-31535 None:intergenic 30.0%
ATGTAAACTGATTAACTGAG+TGG - tig0017277:31554-31573 None:intergenic 30.0%
ACGATATATGGTTGTATGAA+AGG + tig0017277:31616-31635 Msa1360930:three_prime_UTR 30.0%
GTGCATAGTATATTGTTAAG+TGG + tig0017277:31668-31687 Msa1360930:three_prime_UTR 30.0%
TATTGTTAAGTGGCATATAG+AGG + tig0017277:31678-31697 Msa1360930:three_prime_UTR 30.0%
! ATCATGTTAGTTTGATTGCA+CGG + tig0017277:31768-31787 Msa1360930:three_prime_UTR 30.0%
! TTGGGGATTTCATTTAGAAT+TGG + tig0017277:31793-31812 Msa1360930:three_prime_UTR 30.0%
AGAAAACTGAGACTATGTTG+TGG - tig0017277:28034-28053 None:intergenic 35.0%
AATCATGAAGTCAGGGAAAT+TGG + tig0017277:28099-28118 Msa1360930:exon 35.0%
AAACCAAGCTAAACAAGTAG+AGG - tig0017277:28155-28174 None:intergenic 35.0%
!! TCTACTTGTTTAGCTTGGTT+TGG + tig0017277:28154-28173 Msa1360930:CDS 35.0%
CGAAAACGATTCAAGAATGA+AGG - tig0017277:28286-28305 None:intergenic 35.0%
! TTCTTGAATCGTTTTCGTTC+TGG + tig0017277:28289-28308 Msa1360930:CDS 35.0%
GATAAACATTTCCATGAACC+GGG - tig0017277:28330-28349 None:intergenic 35.0%
GGATAAACATTTCCATGAAC+CGG - tig0017277:28331-28350 None:intergenic 35.0%
CATGGAAATGTTTATCCTGT+TGG + tig0017277:28334-28353 Msa1360930:CDS 35.0%
TTTATGCCTTTCTTTCTCCT+AGG + tig0017277:28427-28446 Msa1360930:intron 35.0%
! TCTTCATGCCTTTTCCTATT+TGG + tig0017277:28456-28475 Msa1360930:intron 35.0%
!! TTGGTGCCTGTTTTAAATTG+CGG + tig0017277:28821-28840 Msa1360930:intron 35.0%
CATTGAGTTTCTCGAAATCA+TGG - tig0017277:28890-28909 None:intergenic 35.0%
!! CCCTTACTTTTAACATATGC+AGG + tig0017277:29039-29058 Msa1360930:intron 35.0%
AGTCCAACTTAAAGTTCGTA+TGG + tig0017277:29558-29577 Msa1360930:CDS 35.0%
TTAAAGTTCGTATGGCACTT+GGG + tig0017277:29566-29585 Msa1360930:CDS 35.0%
GGGTATGTAAAGCCTATTTA+AGG - tig0017277:29620-29639 None:intergenic 35.0%
TAAAGAAGTGAGAGTGGTAT+GGG - tig0017277:29640-29659 None:intergenic 35.0%
AAAACCTAAAGAAGTGAGAG+TGG - tig0017277:29646-29665 None:intergenic 35.0%
AAACTATCCCGGAGAAATAA+TGG + tig0017277:29787-29806 Msa1360930:intron 35.0%
! CCGGAGAAATAATGGAAATA+AGG + tig0017277:29795-29814 Msa1360930:intron 35.0%
!! TATTGGCTCATTGCTTAACA+CGG + tig0017277:30821-30840 Msa1360930:intron 35.0%
TTTCCATATGTAGTTGACGA+AGG + tig0017277:30857-30876 Msa1360930:intron 35.0%
ATTCCTTCGTCAACTACATA+TGG - tig0017277:30863-30882 None:intergenic 35.0%
! TCTTCAGGTGCTTCTTTTAT+GGG - tig0017277:30897-30916 None:intergenic 35.0%
! CTTTTTCCATACCAACATAG+AGG - tig0017277:30930-30949 None:intergenic 35.0%
TAATCAGATATGCTTCAGGA+GGG - tig0017277:31046-31065 None:intergenic 35.0%
ATAATCAGATATGCTTCAGG+AGG - tig0017277:31047-31066 None:intergenic 35.0%
CAAATTGTTCTTCCAGAACA+TGG + tig0017277:31176-31195 Msa1360930:intron 35.0%
AACATGGGAAATGTTTGCTT+GGG + tig0017277:31192-31211 Msa1360930:CDS 35.0%
GTGGAAGATGTAAACCTAAT+CGG + tig0017277:31240-31259 Msa1360930:CDS 35.0%
! GCATAGTTCATCAAGAGTTT+TGG - tig0017277:31370-31389 None:intergenic 35.0%
GACAATGAAAAGCAGTTGAT+AGG + tig0017277:31455-31474 Msa1360930:CDS 35.0%
!!! CATCTTTGGATTTTGGAACT+CGG - tig0017277:31502-31521 None:intergenic 35.0%
! ATGCTGACATCTTTGGATTT+TGG - tig0017277:31509-31528 None:intergenic 35.0%
GTTCCTCTACTTGTTTAGCT+TGG + tig0017277:28149-28168 Msa1360930:CDS 40.0%
!!! TCGTTTTCGTTCTGGTTCAT+CGG + tig0017277:28297-28316 Msa1360930:CDS 40.0%
! GTTCTTTTTACGTACCCAAC+AGG - tig0017277:28352-28371 None:intergenic 40.0%
AAGGCATAAAGTGAAACCGA+AGG - tig0017277:28417-28436 None:intergenic 40.0%
AAGCAACCTAGGAGAAAGAA+AGG - tig0017277:28436-28455 None:intergenic 40.0%
GAGTTCGAATATGCACCAAT+TGG + tig0017277:28911-28930 Msa1360930:intron 40.0%
! CAAGAAAATATGGCCTTGGT+GGG - tig0017277:29094-29113 None:intergenic 40.0%
! TCAAGAAAATATGGCCTTGG+TGG - tig0017277:29095-29114 None:intergenic 40.0%
CATCACATTGAAGCCATGTT+AGG - tig0017277:29136-29155 None:intergenic 40.0%
GCAGATCATTACTCATCTCT+TGG + tig0017277:29493-29512 Msa1360930:CDS 40.0%
GTGCCATACGAACTTTAAGT+TGG - tig0017277:29564-29583 None:intergenic 40.0%
CTTAAAGTTCGTATGGCACT+TGG + tig0017277:29565-29584 Msa1360930:CDS 40.0%
!! ACCCTTTGCTAGCCTTAAAT+AGG + tig0017277:29605-29624 Msa1360930:intron 40.0%
! GCCTATTTAAGGCTAGCAAA+GGG - tig0017277:29609-29628 None:intergenic 40.0%
! AGCCTATTTAAGGCTAGCAA+AGG - tig0017277:29610-29629 None:intergenic 40.0%
CTAAAGAAGTGAGAGTGGTA+TGG - tig0017277:29641-29660 None:intergenic 40.0%
CATACCACTCTCACTTCTTT+AGG + tig0017277:29639-29658 Msa1360930:intron 40.0%
AACGCTTATGCTATCCAACT+AGG - tig0017277:29673-29692 None:intergenic 40.0%
GTGTCAGAAGTTGTAACAAC+CGG + tig0017277:29836-29855 Msa1360930:intron 40.0%
TACTGACATAGGCATTTGTG+AGG - tig0017277:30017-30036 None:intergenic 40.0%
! CTGAAACACTTGTTGCAGAA+AGG - tig0017277:30096-30115 None:intergenic 40.0%
ATTCAGCTGTGATATCAAGC+TGG - tig0017277:30297-30316 None:intergenic 40.0%
AGCTTGATATCACAGCTGAA+TGG + tig0017277:30296-30315 Msa1360930:CDS 40.0%
ATATCACAGCTGAATGGTCA+GGG + tig0017277:30302-30321 Msa1360930:CDS 40.0%
GGTTTAGTGAGAAATGTCGT+TGG + tig0017277:30323-30342 Msa1360930:CDS 40.0%
! ATGATTCTTCTCGATTGGCT+TGG + tig0017277:30396-30415 Msa1360930:CDS 40.0%
TACTAATCTCTCGACGACAT+TGG - tig0017277:30425-30444 None:intergenic 40.0%
TCAGCAAACATTACTCAGCA+GGG + tig0017277:30520-30539 Msa1360930:intron 40.0%
!!! TTCTTGCTGTTTTTGACGCT+GGG + tig0017277:30589-30608 Msa1360930:CDS 40.0%
!! CGTTGACATAGTCTTGTTTG+TGG - tig0017277:30731-30750 None:intergenic 40.0%
TAGTTGACGAAGGAATTAGC+TGG + tig0017277:30867-30886 Msa1360930:intron 40.0%
! GTCTTCAGGTGCTTCTTTTA+TGG - tig0017277:30898-30917 None:intergenic 40.0%
ACCAAACTCTTCCTCTATGT+TGG + tig0017277:30916-30935 Msa1360930:CDS 40.0%
ACCAACATAGAGGAAGAGTT+TGG - tig0017277:30920-30939 None:intergenic 40.0%
ACTCTTCCTCTATGTTGGTA+TGG + tig0017277:30921-30940 Msa1360930:CDS 40.0%
TTCAGGAGGGATTTCGAATT+GGG - tig0017277:31033-31052 None:intergenic 40.0%
AGCAAACATTTCCCATGTTC+TGG - tig0017277:31191-31210 None:intergenic 40.0%
GAACATGGGAAATGTTTGCT+TGG + tig0017277:31191-31210 Msa1360930:intron 40.0%
GAAGATGTAAACCTAATCGG+AGG + tig0017277:31243-31262 Msa1360930:CDS 40.0%
ATGTAAACCTAATCGGAGGT+AGG + tig0017277:31247-31266 Msa1360930:intron 40.0%
CTGATGCTTGTTCCTGTTAA+CGG + tig0017277:31294-31313 Msa1360930:intron 40.0%
ATGGATATGTCTGCATACAC+AGG - tig0017277:31413-31432 None:intergenic 40.0%
ATCCATGCTAGACAAAGTGA+TGG + tig0017277:31427-31446 Msa1360930:CDS 40.0%
TACCATCACTTTGTCTAGCA+TGG - tig0017277:31432-31451 None:intergenic 40.0%
!! ATGAAAAGCAGTTGATAGGG+TGG + tig0017277:31459-31478 Msa1360930:CDS 40.0%
! TTAGTTTGATTGCACGGCAT+TGG + tig0017277:31774-31793 Msa1360930:three_prime_UTR 40.0%
TAGTTTGATTGCACGGCATT+GGG + tig0017277:31775-31794 Msa1360930:three_prime_UTR 40.0%
AGGCATGAAGAAAGCAACCT+AGG - tig0017277:28447-28466 None:intergenic 45.0%
ATGGAGGGCCAAATAGGAAA+AGG - tig0017277:28467-28486 None:intergenic 45.0%
CAAAACATGGAGGGCCAAAT+AGG - tig0017277:28473-28492 None:intergenic 45.0%
CATGGGACTAAGTCATTGCT+AGG - tig0017277:29388-29407 None:intergenic 45.0%
GTAGTTATCCGTGTGATGCA+CGG - tig0017277:29431-29450 None:intergenic 45.0%
GATATCACAGCTGAATGGTC+AGG + tig0017277:30301-30320 Msa1360930:CDS 45.0%
CTCAGCAAACATTACTCAGC+AGG + tig0017277:30519-30538 Msa1360930:intron 45.0%
CAGCAAACATTACTCAGCAG+GGG + tig0017277:30521-30540 Msa1360930:intron 45.0%
AGCAAACATTACTCAGCAGG+GGG + tig0017277:30522-30541 Msa1360930:intron 45.0%
! GAGCTGAACTTGTTCTTGGA+GGG + tig0017277:30544-30563 Msa1360930:CDS 45.0%
! AACTTGTTCTTGGAGGGAAG+AGG + tig0017277:30550-30569 Msa1360930:CDS 45.0%
!!! CTTCTTGCTGTTTTTGACGC+TGG + tig0017277:30588-30607 Msa1360930:CDS 45.0%
! AGAGGAAGAGTTTGGTCTTC+AGG - tig0017277:30912-30931 None:intergenic 45.0%
CTTTCGATCTTCCACTTCCT+GGG - tig0017277:31010-31029 None:intergenic 45.0%
CTTCAGGAGGGATTTCGAAT+TGG - tig0017277:31034-31053 None:intergenic 45.0%
!! TGTCAGCATCGACACATGTA+AGG - tig0017277:31158-31177 None:intergenic 45.0%
GTTTGCTTGGGCATTCTAGA+CGG + tig0017277:31204-31223 Msa1360930:CDS 45.0%
!! ATTCTAGACGGCTCTGAAGT+AGG + tig0017277:31216-31235 Msa1360930:CDS 45.0%
AACCTAATCGGAGGTAGGAA+TGG + tig0017277:31252-31271 Msa1360930:intron 45.0%
TGGCTGATCACTTTGTCAGT+TGG - tig0017277:31737-31756 None:intergenic 45.0%
AGTTTGATTGCACGGCATTG+GGG + tig0017277:31776-31795 Msa1360930:three_prime_UTR 45.0%
!!! TTTTTTTTTAATATATAGAT+GGG + tig0017277:28607-28626 Msa1360930:intron 5.0%
! TGGTTCATCGGTTGTGTTCC+CGG + tig0017277:28309-28328 Msa1360930:CDS 50.0%
TCAACATTGGGTACCCACCA+AGG + tig0017277:29078-29097 Msa1360930:CDS 50.0%
AGACACCACATCCGCTTTAC+CGG + tig0017277:29362-29381 Msa1360930:intron 50.0%
TCATTGCTAGGCCGGTAAAG+CGG - tig0017277:29376-29395 None:intergenic 50.0%
GGACTAAGTCATTGCTAGGC+CGG - tig0017277:29384-29403 None:intergenic 50.0%
GCAATGACTTAGTCCCATGC+AGG + tig0017277:29389-29408 Msa1360930:CDS 50.0%
ACGAGGTGGTAGGAAGAATC+TGG - tig0017277:30242-30261 None:intergenic 50.0%
TCTTCCTACCACCTCGTAGA+TGG + tig0017277:30245-30264 Msa1360930:CDS 50.0%
CATTCCATCTACGAGGTGGT+AGG - tig0017277:30252-30271 None:intergenic 50.0%
CAAGCATTCCATCTACGAGG+TGG - tig0017277:30256-30275 None:intergenic 50.0%
CACCTCGTAGATGGAATGCT+TGG + tig0017277:30254-30273 Msa1360930:CDS 50.0%
GTAGATGGAATGCTTGGCCT+TGG + tig0017277:30260-30279 Msa1360930:CDS 50.0%
GGACACTGTTTGAGTGCACA+AGG + tig0017277:30344-30363 Msa1360930:CDS 50.0%
CACTGTTTGAGTGCACAAGG+CGG + tig0017277:30347-30366 Msa1360930:CDS 50.0%
GGCGGAGGATACATCTTCTT+TGG + tig0017277:30365-30384 Msa1360930:CDS 50.0%
! GGAGCTGAACTTGTTCTTGG+AGG + tig0017277:30543-30562 Msa1360930:CDS 50.0%
TTCTTGGAGGGAAGAGGACT+GGG + tig0017277:30556-30575 Msa1360930:CDS 50.0%
CCTGGGAAGCTAAGTGCAAT+AGG - tig0017277:30993-31012 None:intergenic 50.0%
CCTATTGCACTTAGCTTCCC+AGG + tig0017277:30990-31009 Msa1360930:CDS 50.0%
GCTTTCGATCTTCCACTTCC+TGG - tig0017277:31011-31030 None:intergenic 50.0%
TGTCTGCATACACAGGACGA+GGG - tig0017277:31406-31425 None:intergenic 50.0%
ATGTCTGCATACACAGGACG+AGG - tig0017277:31407-31426 None:intergenic 50.0%
GATGACGACGACGACGATGA+AGG - tig0017277:28259-28278 None:intergenic 55.0%
TCGGTTGTGTTCCCGGTTCA+TGG + tig0017277:28316-28335 Msa1360930:CDS 55.0%
ACACGGGTAGCGACCTAACA+TGG + tig0017277:29120-29139 Msa1360930:CDS 55.0%
ACCTGAGAGCAACGCGAACA+AGG - tig0017277:29161-29180 None:intergenic 55.0%
TGATGCACGGATGCACAGAG+GGG - tig0017277:29418-29437 None:intergenic 55.0%
GTGATGCACGGATGCACAGA+GGG - tig0017277:29419-29438 None:intergenic 55.0%
TGTGATGCACGGATGCACAG+AGG - tig0017277:29420-29439 None:intergenic 55.0%
CTGTGCATCCGTGCATCACA+CGG + tig0017277:29420-29439 Msa1360930:CDS 55.0%
GGCCAAGCATTCCATCTACG+AGG - tig0017277:30259-30278 None:intergenic 55.0%
!! ATGGAATGCTTGGCCTTGGC+AGG + tig0017277:30264-30283 Msa1360930:CDS 55.0%
!! TGGAATGCTTGGCCTTGGCA+GGG + tig0017277:30265-30284 Msa1360930:CDS 55.0%
! AGCTGGATTTTCCCCTGCCA+AGG - tig0017277:30280-30299 None:intergenic 55.0%
TGTTTGAGTGCACAAGGCGG+AGG + tig0017277:30350-30369 Msa1360930:CDS 55.0%
! GGGGGAGCTGAACTTGTTCT+TGG + tig0017277:30540-30559 Msa1360930:CDS 55.0%
GTTCTTGGAGGGAAGAGGAC+TGG + tig0017277:30555-30574 Msa1360930:CDS 55.0%
GCACTTAGCTTCCCAGGAAG+TGG + tig0017277:30996-31015 Msa1360930:CDS 55.0%
!! AGACGGCTCTGAAGTAGGAG+TGG + tig0017277:31221-31240 Msa1360930:CDS 55.0%
CGCCATTCCTACCTCCGATT+AGG - tig0017277:31257-31276 None:intergenic 55.0%
! GCCTTGTTCGCGTTGCTCTC+AGG + tig0017277:29157-29176 Msa1360930:CDS 60.0%
CTAGGCCGGTAAAGCGGATG+TGG - tig0017277:29370-29389 None:intergenic 60.0%
CACAGAGGGGATGCCTGCAT+GGG - tig0017277:29405-29424 None:intergenic 60.0%
!! GGAATGCTTGGCCTTGGCAG+GGG + tig0017277:30266-30285 Msa1360930:CDS 60.0%
GGAGGGAAGAGGACTGGGTT+TGG + tig0017277:30561-30580 Msa1360930:CDS 60.0%
GCACAGAGGGGATGCCTGCA+TGG - tig0017277:29406-29425 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0017277 gene 28005 31845 28005 ID=Msa1360930;Name=Msa1360930
tig0017277 mRNA 28005 31845 28005 ID=Msa1360930-mRNA-1;Parent=Msa1360930;Name=Msa1360930-mRNA-1;_AED=0.06;_eAED=0.06;_QI=98|1|1|1|1|1|8|313|447
tig0017277 exon 28005 28356 28005 ID=Msa1360930-mRNA-1:exon:12134;Parent=Msa1360930-mRNA-1
tig0017277 exon 29061 29178 29061 ID=Msa1360930-mRNA-1:exon:12135;Parent=Msa1360930-mRNA-1
tig0017277 exon 29364 29587 29364 ID=Msa1360930-mRNA-1:exon:12136;Parent=Msa1360930-mRNA-1
tig0017277 exon 30217 30441 30217 ID=Msa1360930-mRNA-1:exon:12137;Parent=Msa1360930-mRNA-1
tig0017277 exon 30524 30662 30524 ID=Msa1360930-mRNA-1:exon:12138;Parent=Msa1360930-mRNA-1
tig0017277 exon 30870 31070 30870 ID=Msa1360930-mRNA-1:exon:12139;Parent=Msa1360930-mRNA-1
tig0017277 exon 31192 31264 31192 ID=Msa1360930-mRNA-1:exon:12140;Parent=Msa1360930-mRNA-1
tig0017277 exon 31423 31845 31423 ID=Msa1360930-mRNA-1:exon:12141;Parent=Msa1360930-mRNA-1
tig0017277 five_prime_UTR 28005 28102 28005 ID=Msa1360930-mRNA-1:five_prime_utr;Parent=Msa1360930-mRNA-1
tig0017277 CDS 28103 28356 28103 ID=Msa1360930-mRNA-1:cds;Parent=Msa1360930-mRNA-1
tig0017277 CDS 29061 29178 29061 ID=Msa1360930-mRNA-1:cds;Parent=Msa1360930-mRNA-1
tig0017277 CDS 29364 29587 29364 ID=Msa1360930-mRNA-1:cds;Parent=Msa1360930-mRNA-1
tig0017277 CDS 30217 30441 30217 ID=Msa1360930-mRNA-1:cds;Parent=Msa1360930-mRNA-1
tig0017277 CDS 30524 30662 30524 ID=Msa1360930-mRNA-1:cds;Parent=Msa1360930-mRNA-1
tig0017277 CDS 30870 31070 30870 ID=Msa1360930-mRNA-1:cds;Parent=Msa1360930-mRNA-1
tig0017277 CDS 31192 31264 31192 ID=Msa1360930-mRNA-1:cds;Parent=Msa1360930-mRNA-1
tig0017277 CDS 31423 31532 31423 ID=Msa1360930-mRNA-1:cds;Parent=Msa1360930-mRNA-1
tig0017277 three_prime_UTR 31533 31845 31533 ID=Msa1360930-mRNA-1:three_prime_utr;Parent=Msa1360930-mRNA-1
Gene Sequence

>Msa1360930

ATGAAGTCAGGGAAATTGGTATTCTTTGTTGTTCTACTCTTGTTGAGTTCCTCTACTTGTTTAGCTTGGTTTGGTAGTAGTAGTAGTAGTAGCAAACACAAAATTTCAAGTTCAAGTGCAAATGGAAGAAACTCCATTCTTTCATATGAACAACCTTCATCGTCGTCGTCGTCATCATCGCCTTCATTCTTGAATCGTTTTCGTTCTGGTTCATCGGTTGTGTTCCCGGTTCATGGAAATGTTTATCCTGTTGGGTTTTATAATGTGACTATCAACATTGGGTACCCACCAAGGCCATATTTTCTTGATATTGACACGGGTAGCGACCTAACATGGCTTCAATGTGATGCGCCTTGTTCGCGTTGCTCTCAGACACCACATCCGCTTTACCGGCCTAGCAATGACTTAGTCCCATGCAGGCATCCCCTCTGTGCATCCGTGCATCACACGGATAACTACGACTGTGAAGTCGAGCATCAGTGTGACTATGAAGTTGAGTATGCAGATCATTACTCATCTCTTGGTGTACTTGTCAATGATGTTTATGTTCTCAACTTTACAAATGGAGTCCAACTTAAAGTTCGTATGGCACTTGGATGCGGCTATGATCAGATTTTTCCAGATTCTTCCTACCACCTCGTAGATGGAATGCTTGGCCTTGGCAGGGGAAAATCCAGCTTGATATCACAGCTGAATGGTCAGGGTTTAGTGAGAAATGTCGTTGGACACTGTTTGAGTGCACAAGGCGGAGGATACATCTTCTTTGGAGATGTTTATGATTCTTCTCGATTGGCTTGGACTCCAATGTCGTCGAGAGATTACAAACATTACTCAGCAGGGGGAGCTGAACTTGTTCTTGGAGGGAAGAGGACTGGGTTTGGAAATCTTCTTGCTGTTTTTGACGCTGGGAGTTCTTATACTTACTTCAACTCTAATGCTTATCAAGCACTAATTTCTTGGTTGACGAAGGAATTAGCTGGAAAGCCCATAAAAGAAGCACCTGAAGACCAAACTCTTCCTCTATGTTGGTATGGAAAAAGACCTTTTAGAAGTGTATATGAAGTCAGAAAATACTTCAAGCCTATTGCACTTAGCTTCCCAGGAAGTGGAAGATCGAAAGCCCAATTCGAAATCCCTCCTGAAGCATATCTGATTATATCAAACATGGGAAATGTTTGCTTGGGCATTCTAGACGGCTCTGAAGTAGGAGTGGAAGATGTAAACCTAATCGGAGACATATCCATGCTAGACAAAGTGATGGTATTTGACAATGAAAAGCAGTTGATAGGGTGGACAGCAGCAGATTGTAACCGAGTTCCAAAATCCAAAGATGTCAGCATTTGA
Protein sequence

>Msa1360930

MKSGKLVFFVVLLLLSSSTCLAWFGSSSSSSKHKISSSSANGRNSILSYEQPSSSSSSSSPSFLNRFRSGSSVVFPVHGNVYPVGFYNVTINIGYPPRPYFLDIDTGSDLTWLQCDAPCSRCSQTPHPLYRPSNDLVPCRHPLCASVHHTDNYDCEVEHQCDYEVEYADHYSSLGVLVNDVYVLNFTNGVQLKVRMALGCGYDQIFPDSSYHLVDGMLGLGRGKSSLISQLNGQGLVRNVVGHCLSAQGGGYIFFGDVYDSSRLAWTPMSSRDYKHYSAGGAELVLGGKRTGFGNLLAVFDAGSSYTYFNSNAYQALISWLTKELAGKPIKEAPEDQTLPLCWYGKRPFRSVYEVRKYFKPIALSFPGSGRSKAQFEIPPEAYLIISNMGNVCLGILDGSEVGVEDVNLIGDISMLDKVMVFDNEKQLIGWTAADCNRVPKSKDVSI*