AlfalfaGEDB | Msa1222960

M. sativa cultivar zhongmu-4 / Msa1222960

KEGG: K05350|K07409
GO: GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0010143 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0043170 GO:0044550 GO:0052722 GO:0055114 GO:0071704 GO:1901576
PFAM: PF00067.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1222960	RHN64945.1	99.016	508	5	0	1	508	37	544	0.0	1038

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1222960	sp\|O48928\|C77A3_SOYBN	46.245	506	255	9	9	506	10	506	1.40e-158	463

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0359250	Msa1222960	0.807409	5.127355e-50	-8.615850e-47
Msa0451400	Msa1222960	0.800441	1.438935e-48	-8.615850e-47
Msa0480970	Msa1222960	0.954678	1.719138e-112	-8.615850e-47
Msa0581750	Msa1222960	0.971357	4.906330e-133	-8.615850e-47
Msa0669840	Msa1222960	0.806260	8.966509e-50	-8.615850e-47
Msa0898730	Msa1222960	0.812258	4.641701e-51	-8.615850e-47
Msa1037900	Msa1222960	0.817781	2.759775e-52	-8.615850e-47
Msa1127140	Msa1222960	0.805646	1.207426e-49	-8.615850e-47
Msa1129960	Msa1222960	0.822316	2.527813e-53	-8.615850e-47
Msa1129970	Msa1222960	0.813228	2.846429e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1222960	MtrunA17_Chr4g0074581	99.016	508	5	0	1	508	37	544	0.0	1038
Msa1222960	MtrunA17_Chr1g0198191	46.612	487	249	6	24	506	36	515	8.01e-157	456
Msa1222960	MtrunA17_Chr2g0280121	39.570	465	260	8	52	506	56	509	1.55e-107	330
Msa1222960	MtrunA17_Chr5g0413911	38.697	491	282	8	32	506	41	528	2.15e-107	330
Msa1222960	MtrunA17_Chr2g0280081	39.957	463	259	8	52	506	553	1004	1.28e-105	338
Msa1222960	MtrunA17_Chr2g0280081	37.885	454	263	8	52	497	52	494	3.71e-95	310
Msa1222960	MtrunA17_Chr5g0409051	35.464	485	303	7	30	506	26	508	3.63e-103	318
Msa1222960	MtrunA17_Chr2g0280071	39.175	485	273	9	33	506	63	536	8.05e-101	319
Msa1222960	MtrunA17_Chr8g0387101	38.197	466	264	8	52	506	54	506	3.68e-98	305
Msa1222960	MtrunA17_Chr8g0364651	36.495	485	281	12	33	506	30	498	1.08e-97	304

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1222960	AT1G11600.1	68.493	511	156	4	1	507	1	510	0.0	711
Msa1222960	AT5G04630.1	44.770	478	255	5	32	506	37	508	8.69e-145	426
Msa1222960	AT5G04660.1	44.269	506	273	5	4	506	12	511	3.51e-143	421
Msa1222960	AT3G10560.1	43.852	488	259	6	23	506	36	512	4.76e-143	421
Msa1222960	AT3G10570.1	43.376	468	251	7	44	506	54	512	1.08e-136	405
Msa1222960	AT1G64930.1	39.375	480	282	8	33	506	32	508	1.18e-109	335
Msa1222960	AT1G64940.1	39.375	480	281	7	33	506	33	508	2.08e-108	332
Msa1222960	AT2G12190.1	39.375	480	283	8	33	506	32	509	3.75e-107	329
Msa1222960	AT1G64950.1	38.958	480	283	7	33	506	32	507	1.29e-106	328
Msa1222960	AT3G03470.1	36.492	496	284	10	29	506	27	509	4.74e-98	305

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Find 158 sgRNAs with CRISPR-Local

Find 404 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAGGAAGCTTAGGAA+GGG	0.632530	8_1:-87365036	None:intergenic
AAAATGTGGAGATCCCGAAA+TGG	0.539255	8_1:+87365181	Msa1222960:CDS
AAACATATCCGGATCCTTCC+AGG	0.363313	8_1:-87365892	None:intergenic
AAACATGTTGTTGGCCAAAA+TGG	0.250855	8_1:+87366043	Msa1222960:CDS
AAACCTACCACCAGGAGTTC+CGG	0.343196	8_1:+87364482	Msa1222960:CDS
AAAGTCCCTAATGTCCATGC+TGG	0.591224	8_1:-87366014	None:intergenic
AAAGTTTACGTGCAAAGCGA+TGG	0.522526	8_1:+87365158	Msa1222960:CDS
AACCCGGAACTCCTGGTGGT+AGG	0.651423	8_1:-87364485	None:intergenic
AACCTACCACCAGGAGTTCC+GGG	0.410541	8_1:+87364483	Msa1222960:CDS
AACGGCGCCACCACCACCTG+AGG	0.564095	8_1:-87364434	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAGAAGAGTAATTGTGAGA+AGG	-	chr8_1:87364322-87364341	None:intergenic	30.0%
TGTTAAGAAGAAGGAAAGAA+AGG	-	chr8_1:87364366-87364385	None:intergenic	30.0%
CAAAATGAAGCACATGAAAA+TGG	+	chr8_1:87364882-87364901	Msa1222960:CDS	30.0%
GTTAAAACCGGTAAAAAATC+AGG	-	chr8_1:87365053-87365072	None:intergenic	30.0%
TTAGACATAAGATGGTCAAA+GGG	-	chr8_1:87365281-87365300	None:intergenic	30.0%
TTTAGACATAAGATGGTCAA+AGG	-	chr8_1:87365282-87365301	None:intergenic	30.0%
TTCACAATTGCACATAGATA+AGG	-	chr8_1:87365732-87365751	None:intergenic	30.0%
TTATCTATGTGCAATTGTGA+AGG	+	chr8_1:87365731-87365750	Msa1222960:CDS	30.0%
GTAACAATTTAAATGGACCT+AGG	-	chr8_1:87366173-87366192	None:intergenic	30.0%
TAAGAAGAGTAATTGTGAGA+AGG	-	chr8_1:87364322-87364341	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	87364319	87366492	87364319	ID=Msa1222960;Name=Msa1222960
chr8_1	mRNA	87364319	87366492	87364319	ID=Msa1222960-mRNA-1;Parent=Msa1222960;Name=Msa1222960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=71\|1\|1\|1\|1\|1\|2\|308\|508
chr8_1	exon	87364319	87365202	87364319	ID=Msa1222960-mRNA-1:exon:31464;Parent=Msa1222960-mRNA-1
chr8_1	exon	87365471	87366492	87365471	ID=Msa1222960-mRNA-1:exon:31465;Parent=Msa1222960-mRNA-1
chr8_1	five_prime_UTR	87364319	87364389	87364319	ID=Msa1222960-mRNA-1:five_prime_utr;Parent=Msa1222960-mRNA-1
chr8_1	CDS	87364390	87365202	87364390	ID=Msa1222960-mRNA-1:cds;Parent=Msa1222960-mRNA-1
chr8_1	CDS	87365471	87366184	87365471	ID=Msa1222960-mRNA-1:cds;Parent=Msa1222960-mRNA-1
chr8_1	three_prime_UTR	87366185	87366492	87366185	ID=Msa1222960-mRNA-1:three_prime_utr;Parent=Msa1222960-mRNA-1

Gene Sequence

>Msa1222960

ATGGAGTTCATAGACGTTATAATGTTCCTTTTTGCCCTCGCCTTCCTCAGGTGGTGGTGGCGCCGTTGGTCAAAGACCGGGGGCGGCACTAAAAACCTACCACCAGGAGTTCCGGGTTGGCCAATCGTTGGCAATCTCTTCCAAGTTATCCTCCAACGCCGTCCTTTCATCTACATAGTTCGTGATTTACGTCTAAAATACGGTCCAATTTTCACAATGCAAATGGGTCAACGCACCTTGATTATAGTCACAGAAGCTGACCTCATCCATGAAGCCTTAATCCAACGAGGCCCACAGTTTGCAAGTAGGCCTAAAGATTCTCCCATTCGTCTCATCTTCAGCATGGGAAAGTGTGCCATCAACTCCGCCGAATACGGTCCACTCTGGCGCTCCCTCCGCCGCAACCTTGTGACGGAGATGATATCACCACTTAGAGTAAAACAGTGCAGTTGGATCAGAAAATGGGCCATGGAAGCTCACATGAGAAGGATCCAAAATGAAGCACATGAAAATGGTTTTGTTGAAGTGATGAGTAACTGCAGACTCACCATTTGTAGCATTCTTATATGTCTATGTTTCGGTGCGAAAATAACAGAGGAAAGAATCAGAGATATTGAGAGTGTTCTCAAAGAAGTCATGCTCATTACCCTTCCTAAGCTTCCTGATTTTTTACCGGTTTTAACACCATTATTCCGCGGCCATGTGAAGGAAGCGAAGAAGCTGAGGAAGAAACAGCTGGAGCTTATAGCGCCGTTGATAAGGAAACGGAAAGTTTACGTGCAAAGCGATGGAAAATGTGGAGATCCCGAAATGGTGAGTGCCATTGGTGCTGCTTATGTTGATTCATTGTTTGGTTTGGAAGTGCCTAGTAGAGGGAGATTGGGAGAAGAGGAATTAGTTACGCTCGTGTCAGAGATTATCAGTGCTGGTACTGACACGAGCGCAACGGCCATTGAATGGGCTTTGCTTCATTTAGTGATGGATCAAGAAATTCAAGAGAGGTTGTATAGAGAAATTGTTGATTGTGTTGGAAAATGCGGTGTTGTTATGGACAGTGACGTTGAAAAAATGCCTTATCTATGTGCAATTGTGAAGGAAACTTTTCGGCGTCATCCTCCTAGCCATTTTGTGCTTTCGCACGCGGCTACGGAGGAGACAAAGCTTGGTGGATACACTATTCCGGCAGATGCAAGTGTGGAGTTTTACACGGCTTGGTTGTCGGAAAATCCAAATACCTGGAAGGATCCGGATATGTTTCGACCCGAGAGGTTTTTGAATGGGGATGGAGTTGATGTTGATATCACAGGAACTAAAGAGATGAAGATGATGCCTTTTGGTGCTGGAAGAAGGATTTGTCCAGCATGGACATTAGGGACTTTGCATATAAACATGTTGTTGGCCAAAATGGTTCTTGCTTTTAGGTGGTTGCCGGTTCCAAATTCCCCACCCGACCCAACTGAGACATTTGCTTTCACCGTTGTTATGAAGAATCCTCTCAAGGCTGTTATTGTTCCTAGGTCCATTTAA

Protein sequence

>Msa1222960

MEFIDVIMFLFALAFLRWWWRRWSKTGGGTKNLPPGVPGWPIVGNLFQVILQRRPFIYIVRDLRLKYGPIFTMQMGQRTLIIVTEADLIHEALIQRGPQFASRPKDSPIRLIFSMGKCAINSAEYGPLWRSLRRNLVTEMISPLRVKQCSWIRKWAMEAHMRRIQNEAHENGFVEVMSNCRLTICSILICLCFGAKITEERIRDIESVLKEVMLITLPKLPDFLPVLTPLFRGHVKEAKKLRKKQLELIAPLIRKRKVYVQSDGKCGDPEMVSAIGAAYVDSLFGLEVPSRGRLGEEELVTLVSEIISAGTDTSATAIEWALLHLVMDQEIQERLYREIVDCVGKCGVVMDSDVEKMPYLCAIVKETFRRHPPSHFVLSHAATEETKLGGYTIPADASVEFYTAWLSENPNTWKDPDMFRPERFLNGDGVDVDITGTKEMKMMPFGAGRRICPAWTLGTLHINMLLAKMVLAFRWLPVPNSPPDPTETFAFTVVMKNPLKAVIVPRSI*