Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1223290 | XP_039689784.1 | 91.367 | 139 | 7 | 2 | 1 | 138 | 1 | 135 | 3.70e-74 | 227 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0132530 | Msa1223290 | 0.804522 | 2.074503e-49 | -8.615850e-47 |
| Msa0132550 | Msa1223290 | 0.858790 | 6.468844e-63 | -8.615850e-47 |
| Msa0200460 | Msa1223290 | 0.844045 | 9.639832e-59 | -8.615850e-47 |
| Msa1015930 | Msa1223290 | 0.815267 | 1.009281e-51 | -8.615850e-47 |
| Msa1223290 | Msa1263840 | 0.958780 | 1.008060e-116 | -8.615850e-47 |
| Msa1223290 | Msa1263880 | 0.934467 | 3.859364e-96 | -8.615850e-47 |
| Msa1223290 | Msa1367250 | 0.935729 | 5.358444e-97 | -8.615850e-47 |
| Msa1223290 | Msa1430360 | 0.869438 | 3.096901e-66 | -8.615850e-47 |
| Msa0887620 | Msa1223290 | 0.859427 | 4.168311e-63 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1223290 | MtrunA17_Chr4g0074761 | 91.367 | 139 | 7 | 2 | 1 | 138 | 1 | 135 | 3.40e-78 | 227 |
| Msa1223290 | MtrunA17_Chr4g0074741 | 69.014 | 142 | 34 | 5 | 1 | 138 | 1 | 136 | 1.36e-57 | 175 |
| Msa1223290 | MtrunA17_Chr2g0288321 | 49.650 | 143 | 49 | 6 | 1 | 138 | 1 | 125 | 1.52e-30 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTTTGCCTTCCTTGATTC+AGG | 0.178873 | 8_1:+87702294 | None:intergenic |
| GATGATTATTTGTAATTTCA+TGG | 0.249624 | 8_1:+87702007 | None:intergenic |
| GAGCGAACTCGCTGAATTAT+TGG | 0.258439 | 8_1:-87702145 | Msa1223290:CDS |
| AAGTTAATTAACATTCATTC+AGG | 0.322588 | 8_1:+87701958 | None:intergenic |
| CAGAAGGAGATTGATGTATT+AGG | 0.330408 | 8_1:-87702222 | Msa1223290:CDS |
| CTGCAGAAGAAGTTCTGTTT+AGG | 0.332908 | 8_1:-87702047 | Msa1223290:CDS |
| GCAGCAGATGAAGAAGAAAA+AGG | 0.371748 | 8_1:-87702079 | Msa1223290:CDS |
| AGCGAACTCGCTGAATTATT+GGG | 0.393306 | 8_1:-87702144 | Msa1223290:CDS |
| TGGAGTGGGTCGGAGAGGAC+TGG | 0.403882 | 8_1:-87702335 | Msa1223290:CDS |
| ACCTTGCTTGATGATGGATA+TGG | 0.406319 | 8_1:+87702273 | None:intergenic |
| TCAAACACCTTGCTTGATGA+TGG | 0.416575 | 8_1:+87702267 | None:intergenic |
| TTCTTCTTCATCTGCTGCTG+TGG | 0.454237 | 8_1:+87702084 | None:intergenic |
| GGAGTGGGTCGGAGAGGACT+GGG | 0.460819 | 8_1:-87702334 | Msa1223290:CDS |
| GAGAGGACTGGGAATCTTTG+AGG | 0.465583 | 8_1:-87702323 | Msa1223290:CDS |
| TTGCAAACCATCATCTTCAA+TGG | 0.494191 | 8_1:-87702355 | Msa1223290:CDS |
| ACCCACTCCATTGAAGATGA+TGG | 0.494750 | 8_1:+87702348 | None:intergenic |
| AAATTACAAATAATCATCAC+TGG | 0.529214 | 8_1:-87702002 | Msa1223290:CDS |
| AACCATCATCTTCAATGGAG+TGG | 0.534475 | 8_1:-87702350 | Msa1223290:CDS |
| TGAGGTCTAAACCTGAATCA+AGG | 0.541762 | 8_1:-87702305 | Msa1223290:CDS |
| TCAGGAATAGTCTCTAGCAC+TGG | 0.560488 | 8_1:+87701976 | None:intergenic |
| ACCATCATCTTCAATGGAGT+GGG | 0.563387 | 8_1:-87702349 | Msa1223290:CDS |
| GAGCTTCTTGTGACGCAAGT+GGG | 0.570204 | 8_1:-87702191 | Msa1223290:CDS |
| AGAGCTTCTTGTGACGCAAG+TGG | 0.586202 | 8_1:-87702192 | Msa1223290:CDS |
| TTCTTGTGACGCAAGTGGGA+AGG | 0.592803 | 8_1:-87702187 | Msa1223290:CDS |
| GTCTAAACCTGAATCAAGGA+AGG | 0.611894 | 8_1:-87702301 | Msa1223290:CDS |
| GCCATATCCATCATCAAGCA+AGG | 0.615763 | 8_1:-87702274 | Msa1223290:CDS |
| TAGTCTTGATCATCATCAGA+AGG | 0.617267 | 8_1:-87702238 | Msa1223290:CDS |
| TTCAATGGAGTGGGTCGGAG+AGG | 0.622877 | 8_1:-87702340 | Msa1223290:CDS |
| TCATCTTCAATGGAGTGGGT+CGG | 0.639140 | 8_1:-87702345 | Msa1223290:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTGTAATTTCATGGTTTT+TGG | + | chr8_1:87702327-87702346 | None:intergenic | 20.0% |
| !! | GATGATTATTTGTAATTTCA+TGG | + | chr8_1:87702334-87702353 | None:intergenic | 20.0% |
| !! | AAATTACAAATAATCATCAC+TGG | - | chr8_1:87702336-87702355 | Msa1223290:CDS | 20.0% |
| TTGCAAACCATCATCTTCAA+TGG | - | chr8_1:87701983-87702002 | Msa1223290:CDS | 35.0% | |
| ! | TAGTCTTGATCATCATCAGA+AGG | - | chr8_1:87702100-87702119 | Msa1223290:CDS | 35.0% |
| CAGAAGGAGATTGATGTATT+AGG | - | chr8_1:87702116-87702135 | Msa1223290:CDS | 35.0% | |
| AACCATCATCTTCAATGGAG+TGG | - | chr8_1:87701988-87702007 | Msa1223290:CDS | 40.0% | |
| ACCATCATCTTCAATGGAGT+GGG | - | chr8_1:87701989-87702008 | Msa1223290:CDS | 40.0% | |
| TGAGGTCTAAACCTGAATCA+AGG | - | chr8_1:87702033-87702052 | Msa1223290:CDS | 40.0% | |
| GTCTAAACCTGAATCAAGGA+AGG | - | chr8_1:87702037-87702056 | Msa1223290:CDS | 40.0% | |
| !! | ACCTTGCTTGATGATGGATA+TGG | + | chr8_1:87702068-87702087 | None:intergenic | 40.0% |
| TCAAACACCTTGCTTGATGA+TGG | + | chr8_1:87702074-87702093 | None:intergenic | 40.0% | |
| AGCGAACTCGCTGAATTATT+GGG | - | chr8_1:87702194-87702213 | Msa1223290:CDS | 40.0% | |
| GCAGCAGATGAAGAAGAAAA+AGG | - | chr8_1:87702259-87702278 | Msa1223290:CDS | 40.0% | |
| CTGCAGAAGAAGTTCTGTTT+AGG | - | chr8_1:87702291-87702310 | Msa1223290:CDS | 40.0% | |
| ACCCACTCCATTGAAGATGA+TGG | + | chr8_1:87701993-87702012 | None:intergenic | 45.0% | |
| TCATCTTCAATGGAGTGGGT+CGG | - | chr8_1:87701993-87702012 | Msa1223290:CDS | 45.0% | |
| GCCATATCCATCATCAAGCA+AGG | - | chr8_1:87702064-87702083 | Msa1223290:CDS | 45.0% | |
| GAGCGAACTCGCTGAATTAT+TGG | - | chr8_1:87702193-87702212 | Msa1223290:CDS | 45.0% | |
| TTCTTCTTCATCTGCTGCTG+TGG | + | chr8_1:87702257-87702276 | None:intergenic | 45.0% | |
| TCAGGAATAGTCTCTAGCAC+TGG | + | chr8_1:87702365-87702384 | None:intergenic | 45.0% | |
| GAGAGGACTGGGAATCTTTG+AGG | - | chr8_1:87702015-87702034 | Msa1223290:CDS | 50.0% | |
| GGCTTTGCCTTCCTTGATTC+AGG | + | chr8_1:87702047-87702066 | None:intergenic | 50.0% | |
| AGAGCTTCTTGTGACGCAAG+TGG | - | chr8_1:87702146-87702165 | Msa1223290:CDS | 50.0% | |
| GAGCTTCTTGTGACGCAAGT+GGG | - | chr8_1:87702147-87702166 | Msa1223290:CDS | 50.0% | |
| TTCTTGTGACGCAAGTGGGA+AGG | - | chr8_1:87702151-87702170 | Msa1223290:CDS | 50.0% | |
| TTCAATGGAGTGGGTCGGAG+AGG | - | chr8_1:87701998-87702017 | Msa1223290:CDS | 55.0% | |
| TGGAGTGGGTCGGAGAGGAC+TGG | - | chr8_1:87702003-87702022 | Msa1223290:CDS | 65.0% | |
| GGAGTGGGTCGGAGAGGACT+GGG | - | chr8_1:87702004-87702023 | Msa1223290:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 87701972 | 87702388 | 87701972 | ID=Msa1223290;Name=Msa1223290 |
| chr8_1 | mRNA | 87701972 | 87702388 | 87701972 | ID=Msa1223290-mRNA-1;Parent=Msa1223290;Name=Msa1223290-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|138 |
| chr8_1 | exon | 87701972 | 87702388 | 87701972 | ID=Msa1223290-mRNA-1:exon:31784;Parent=Msa1223290-mRNA-1 |
| chr8_1 | CDS | 87701972 | 87702388 | 87701972 | ID=Msa1223290-mRNA-1:cds;Parent=Msa1223290-mRNA-1 |
| Gene Sequence |
| Protein sequence |
