Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1367250 | XP_039689784.1 | 91.367 | 139 | 7 | 2 | 1 | 138 | 1 | 135 | 3.70e-74 | 227 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1367250 | G7JJ62 | 91.367 | 139 | 7 | 2 | 1 | 138 | 1 | 135 | 1.77e-74 | 227 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1367250 | Msa1430360 | 0.869948 | 2.110678e-66 | -8.615850e-47 |
| Msa0132530 | Msa1367250 | 0.806771 | 6.997666e-50 | -8.615850e-47 |
| Msa0132540 | Msa1367250 | 0.802010 | 6.873016e-49 | -8.615850e-47 |
| Msa0132550 | Msa1367250 | 0.862228 | 5.880266e-64 | -8.615850e-47 |
| Msa1263840 | Msa1367250 | 0.896931 | 2.296446e-76 | -8.615850e-47 |
| Msa1263880 | Msa1367250 | 0.874797 | 5.099862e-68 | -8.615850e-47 |
| Msa0410150 | Msa1367250 | 0.800881 | 1.170262e-48 | -8.615850e-47 |
| Msa1015930 | Msa1367250 | 0.814797 | 1.282962e-51 | -8.615850e-47 |
| Msa1223290 | Msa1367250 | 0.935729 | 5.358444e-97 | -8.615850e-47 |
| Msa0887620 | Msa1367250 | 0.859860 | 3.086915e-63 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1367250 | MtrunA17_Chr4g0074761 | 91.367 | 139 | 7 | 2 | 1 | 138 | 1 | 135 | 3.40e-78 | 227 |
| Msa1367250 | MtrunA17_Chr4g0074741 | 69.014 | 142 | 34 | 5 | 1 | 138 | 1 | 136 | 1.36e-57 | 175 |
| Msa1367250 | MtrunA17_Chr2g0288321 | 49.650 | 143 | 49 | 6 | 1 | 138 | 1 | 125 | 1.52e-30 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTTTGCCTTCCTTGATTC+AGG | 0.178873 | tig0018511:+192597 | None:intergenic |
| GATGATTATTTGTAATTTCA+TGG | 0.249624 | tig0018511:+192310 | None:intergenic |
| AAGCGAACTCGCTGAATTAT+TGG | 0.251619 | tig0018511:-192448 | Msa1367250:CDS |
| AAGTTAATTAACATTCATTC+AGG | 0.322588 | tig0018511:+192261 | None:intergenic |
| CAGAAGGAGATTGATGTATT+AGG | 0.330408 | tig0018511:-192525 | Msa1367250:CDS |
| CTGCAGAAGAAGTTCTGTTT+AGG | 0.332908 | tig0018511:-192350 | Msa1367250:CDS |
| GCAGCAGATGAAGAAGAAAA+AGG | 0.371748 | tig0018511:-192382 | Msa1367250:CDS |
| AGCGAACTCGCTGAATTATT+GGG | 0.386486 | tig0018511:-192447 | Msa1367250:CDS |
| TGGAGTGGGTCGGAGAGGAC+TGG | 0.403882 | tig0018511:-192638 | Msa1367250:CDS |
| ACCTTGCTTGATGATGGATA+TGG | 0.406319 | tig0018511:+192576 | None:intergenic |
| TCAAACACCTTGCTTGATGA+TGG | 0.416575 | tig0018511:+192570 | None:intergenic |
| TTCTTCTTCATCTGCTGCTG+TGG | 0.454237 | tig0018511:+192387 | None:intergenic |
| GGAGTGGGTCGGAGAGGACT+GGG | 0.460819 | tig0018511:-192637 | Msa1367250:CDS |
| GAGAGGACTGGGAATCTTTG+AGG | 0.465583 | tig0018511:-192626 | Msa1367250:CDS |
| TTGCAAACCATCATCTTCAA+TGG | 0.494191 | tig0018511:-192658 | Msa1367250:CDS |
| ACCCACTCCATTGAAGATGA+TGG | 0.494750 | tig0018511:+192651 | None:intergenic |
| AAATTACAAATAATCATCAC+TGG | 0.529214 | tig0018511:-192305 | Msa1367250:CDS |
| AACCATCATCTTCAATGGAG+TGG | 0.534475 | tig0018511:-192653 | Msa1367250:CDS |
| TGAGGTCTAAACCTGAATCA+AGG | 0.541762 | tig0018511:-192608 | Msa1367250:CDS |
| TCAGGAATAGTCTCTAGCAC+TGG | 0.560488 | tig0018511:+192279 | None:intergenic |
| ACCATCATCTTCAATGGAGT+GGG | 0.563387 | tig0018511:-192652 | Msa1367250:CDS |
| GAGCTTCTTGTGACGCAAGT+GGG | 0.570204 | tig0018511:-192494 | Msa1367250:CDS |
| AGAGCTTCTTGTGACGCAAG+TGG | 0.586202 | tig0018511:-192495 | Msa1367250:CDS |
| TTCTTGTGACGCAAGTGGGA+AGG | 0.592803 | tig0018511:-192490 | Msa1367250:CDS |
| GTCTAAACCTGAATCAAGGA+AGG | 0.611894 | tig0018511:-192604 | Msa1367250:CDS |
| GCCATATCCATCATCAAGCA+AGG | 0.615763 | tig0018511:-192577 | Msa1367250:CDS |
| TAGTCTTGATCATCATCAGA+AGG | 0.617267 | tig0018511:-192541 | Msa1367250:CDS |
| TTCAATGGAGTGGGTCGGAG+AGG | 0.622877 | tig0018511:-192643 | Msa1367250:CDS |
| TCATCTTCAATGGAGTGGGT+CGG | 0.639140 | tig0018511:-192648 | Msa1367250:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTGTAATTTCATGGTTTT+TGG | + | tig0018511:192630-192649 | None:intergenic | 20.0% |
| !! | GATGATTATTTGTAATTTCA+TGG | + | tig0018511:192637-192656 | None:intergenic | 20.0% |
| !! | AAATTACAAATAATCATCAC+TGG | - | tig0018511:192639-192658 | Msa1367250:CDS | 20.0% |
| TTGCAAACCATCATCTTCAA+TGG | - | tig0018511:192286-192305 | Msa1367250:CDS | 35.0% | |
| ! | TAGTCTTGATCATCATCAGA+AGG | - | tig0018511:192403-192422 | Msa1367250:CDS | 35.0% |
| CAGAAGGAGATTGATGTATT+AGG | - | tig0018511:192419-192438 | Msa1367250:CDS | 35.0% | |
| AACCATCATCTTCAATGGAG+TGG | - | tig0018511:192291-192310 | Msa1367250:CDS | 40.0% | |
| ACCATCATCTTCAATGGAGT+GGG | - | tig0018511:192292-192311 | Msa1367250:CDS | 40.0% | |
| TGAGGTCTAAACCTGAATCA+AGG | - | tig0018511:192336-192355 | Msa1367250:CDS | 40.0% | |
| GTCTAAACCTGAATCAAGGA+AGG | - | tig0018511:192340-192359 | Msa1367250:CDS | 40.0% | |
| !! | ACCTTGCTTGATGATGGATA+TGG | + | tig0018511:192371-192390 | None:intergenic | 40.0% |
| TCAAACACCTTGCTTGATGA+TGG | + | tig0018511:192377-192396 | None:intergenic | 40.0% | |
| AAGCGAACTCGCTGAATTAT+TGG | - | tig0018511:192496-192515 | Msa1367250:CDS | 40.0% | |
| AGCGAACTCGCTGAATTATT+GGG | - | tig0018511:192497-192516 | Msa1367250:CDS | 40.0% | |
| GCAGCAGATGAAGAAGAAAA+AGG | - | tig0018511:192562-192581 | Msa1367250:CDS | 40.0% | |
| CTGCAGAAGAAGTTCTGTTT+AGG | - | tig0018511:192594-192613 | Msa1367250:CDS | 40.0% | |
| ACCCACTCCATTGAAGATGA+TGG | + | tig0018511:192296-192315 | None:intergenic | 45.0% | |
| TCATCTTCAATGGAGTGGGT+CGG | - | tig0018511:192296-192315 | Msa1367250:CDS | 45.0% | |
| GCCATATCCATCATCAAGCA+AGG | - | tig0018511:192367-192386 | Msa1367250:CDS | 45.0% | |
| TTCTTCTTCATCTGCTGCTG+TGG | + | tig0018511:192560-192579 | None:intergenic | 45.0% | |
| TCAGGAATAGTCTCTAGCAC+TGG | + | tig0018511:192668-192687 | None:intergenic | 45.0% | |
| GAGAGGACTGGGAATCTTTG+AGG | - | tig0018511:192318-192337 | Msa1367250:CDS | 50.0% | |
| GGCTTTGCCTTCCTTGATTC+AGG | + | tig0018511:192350-192369 | None:intergenic | 50.0% | |
| AGAGCTTCTTGTGACGCAAG+TGG | - | tig0018511:192449-192468 | Msa1367250:CDS | 50.0% | |
| GAGCTTCTTGTGACGCAAGT+GGG | - | tig0018511:192450-192469 | Msa1367250:CDS | 50.0% | |
| TTCTTGTGACGCAAGTGGGA+AGG | - | tig0018511:192454-192473 | Msa1367250:CDS | 50.0% | |
| TTCAATGGAGTGGGTCGGAG+AGG | - | tig0018511:192301-192320 | Msa1367250:CDS | 55.0% | |
| TGGAGTGGGTCGGAGAGGAC+TGG | - | tig0018511:192306-192325 | Msa1367250:CDS | 65.0% | |
| GGAGTGGGTCGGAGAGGACT+GGG | - | tig0018511:192307-192326 | Msa1367250:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018511 | gene | 192275 | 192691 | 192275 | ID=Msa1367250;Name=Msa1367250 |
| tig0018511 | mRNA | 192275 | 192691 | 192275 | ID=Msa1367250-mRNA-1;Parent=Msa1367250;Name=Msa1367250-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|138 |
| tig0018511 | exon | 192275 | 192691 | 192275 | ID=Msa1367250-mRNA-1:exon:15479;Parent=Msa1367250-mRNA-1 |
| tig0018511 | CDS | 192275 | 192691 | 192275 | ID=Msa1367250-mRNA-1:cds;Parent=Msa1367250-mRNA-1 |
| Gene Sequence |
| Protein sequence |
