Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1230210 | KEH18080.1 | 97.196 | 107 | 3 | 0 | 1 | 107 | 28 | 134 | 1.09e-69 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0767010 | Msa1230210 | 0.815573 | 8.627580e-52 | -8.615850e-47 |
| Msa0800400 | Msa1230210 | 0.805267 | 1.449786e-49 | -8.615850e-47 |
| Msa0156780 | Msa1230210 | 0.806328 | 8.678575e-50 | -8.615850e-47 |
| Msa1230210 | Msa1310780 | 0.887134 | 1.863273e-72 | -8.615850e-47 |
| Msa0426860 | Msa1230210 | 0.801690 | 7.993208e-49 | -8.615850e-47 |
| Msa0426890 | Msa1230210 | 0.803776 | 2.966777e-49 | -8.615850e-47 |
| Msa1070930 | Msa1230210 | 0.809755 | 1.617383e-50 | -8.615850e-47 |
| Msa1121580 | Msa1230210 | 0.814356 | 1.606667e-51 | -8.615850e-47 |
| Msa0569630 | Msa1230210 | 0.803112 | 4.071842e-49 | -8.615850e-47 |
| Msa0570310 | Msa1230210 | 0.810463 | 1.138304e-50 | -8.615850e-47 |
| Msa0899080 | Msa1230210 | 0.802223 | 6.213475e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1230210 | MtrunA17_Chr8g0338051 | 96.262 | 107 | 4 | 0 | 1 | 107 | 218 | 324 | 1.68e-70 | 213 |
| Msa1230210 | MtrunA17_Chr4g0009941 | 34.951 | 103 | 48 | 3 | 3 | 101 | 161 | 248 | 6.53e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1230210 | AT4G31530.1 | 74.528 | 106 | 27 | 0 | 1 | 106 | 218 | 323 | 3.80e-56 | 176 |
| Msa1230210 | AT4G31530.2 | 65.833 | 120 | 27 | 1 | 1 | 106 | 218 | 337 | 2.90e-52 | 167 |
Find 25 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAGTTAACTTCATATATT+TGG | 0.175603 | 8_2:+6178836 | None:intergenic |
| TCTTACCTAATTATAGTATA+TGG | 0.230550 | 8_2:+6179191 | None:intergenic |
| GCTTTCCATATACTATAATT+AGG | 0.245017 | 8_2:-6179196 | Msa1230210:intron |
| GAGGAATTTCTTCAAAATTC+TGG | 0.262366 | 8_2:-6179218 | Msa1230210:CDS |
| GGTCCATCTGTCAACCTTCC+AGG | 0.296760 | 8_2:+6179115 | None:intergenic |
| CACTTCTGAGCATCATTTCC+AGG | 0.302200 | 8_2:+6178586 | None:intergenic |
| CAACGGCGAGCAGTGCTTAT+AGG | 0.332728 | 8_2:-6179046 | Msa1230210:CDS |
| GATGGACCATACACATCTTA+TGG | 0.378844 | 8_2:-6179100 | Msa1230210:CDS |
| ATGTTTCAGGGTGAAGGTCC+TGG | 0.459105 | 8_2:-6178604 | Msa1230210:intron |
| ACATTACTCAAAGCAACAGC+TGG | 0.493897 | 8_2:-6179070 | Msa1230210:CDS |
| TTCAGCAACCACAATCCTAC+TGG | 0.542318 | 8_2:+6178897 | None:intergenic |
| GGTTGCTGAAGCTTGCGTAC+AGG | 0.566427 | 8_2:-6178884 | Msa1230210:CDS |
| AGACCTGGAAGGTTGACAGA+TGG | 0.569259 | 8_2:-6179118 | Msa1230210:CDS |
| ATGCATATGTTTCAGGGTGA+AGG | 0.572654 | 8_2:-6178610 | Msa1230210:intron |
| TTGAGACCATAAGATGTGTA+TGG | 0.590868 | 8_2:+6179094 | None:intergenic |
| CGAGCAGTGCTTATAGGTCA+AGG | 0.592563 | 8_2:-6179040 | Msa1230210:intron |
| GAAGTGGCAAGAATTATTTG+TGG | 0.603707 | 8_2:-6178569 | Msa1230210:CDS |
| ATATGAAGTTAACTCTGTTG+AGG | 0.604047 | 8_2:-6178830 | Msa1230210:intron |
| CTGGAAATGATGCTCAGAAG+TGG | 0.606575 | 8_2:-6178585 | Msa1230210:CDS |
| TCAAAGCAACAGCTGGGCAA+CGG | 0.612805 | 8_2:-6179063 | Msa1230210:CDS |
| CTTTCAGGAGATAAACTAGT+GGG | 0.632068 | 8_2:-6178925 | Msa1230210:intron |
| GGGTGAAGCCAGTAGGATTG+TGG | 0.633472 | 8_2:-6178905 | Msa1230210:CDS |
| CATTACTCAAAGCAACAGCT+GGG | 0.641871 | 8_2:-6179069 | Msa1230210:CDS |
| AACTAGTGGGTGAAGCCAGT+AGG | 0.661026 | 8_2:-6178912 | Msa1230210:CDS |
| GCTTTCAGGAGATAAACTAG+TGG | 0.695591 | 8_2:-6178926 | Msa1230210:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAATAATTAAGAAATTTA+CGG | + | chr8_2:6179064-6179083 | None:intergenic | 10.0% |
| !! | TCTTACCTAATTATAGTATA+TGG | + | chr8_2:6178588-6178607 | None:intergenic | 20.0% |
| !!! | ATTGATAACCAAAATGTAAA+TGG | - | chr8_2:6178630-6178649 | Msa1230210:intron | 20.0% |
| !!! | TCTAAAATCCATTTACATTT+TGG | + | chr8_2:6178641-6178660 | None:intergenic | 20.0% |
| !!! | ACTTTAAGCAAAAATACTTT+TGG | + | chr8_2:6178812-6178831 | None:intergenic | 20.0% |
| !!! | TTTCTATTGTAATTTTGTTG+AGG | - | chr8_2:6178974-6178993 | Msa1230210:intron | 20.0% |
| !! | TTTAGTGTAAATATCAAATG+CGG | - | chr8_2:6179106-6179125 | Msa1230210:CDS | 20.0% |
| ! | GCTTTCCATATACTATAATT+AGG | - | chr8_2:6178580-6178599 | Msa1230210:CDS | 25.0% |
| !!! | AGCAAAAATACTTTTGGAAA+AGG | + | chr8_2:6178806-6178825 | None:intergenic | 25.0% |
| !! | AAGTGTTAGAAATTTTGTGA+CGG | - | chr8_2:6178828-6178847 | Msa1230210:intron | 25.0% |
| ! | CAGAGTTAACTTCATATATT+TGG | + | chr8_2:6178943-6178962 | None:intergenic | 25.0% |
| ! | GAGGTAAAAATAATGATACA+TGG | - | chr8_2:6178993-6179012 | Msa1230210:intron | 25.0% |
| !!! | TACGGTTTTGATTCTATAAT+GGG | + | chr8_2:6179046-6179065 | None:intergenic | 25.0% |
| !!! | TTACGGTTTTGATTCTATAA+TGG | + | chr8_2:6179047-6179066 | None:intergenic | 25.0% |
| ! | TTAGTGTAAATATCAAATGC+GGG | - | chr8_2:6179107-6179126 | Msa1230210:CDS | 25.0% |
| GAGGAATTTCTTCAAAATTC+TGG | - | chr8_2:6178558-6178577 | Msa1230210:CDS | 30.0% | |
| !! | ATGTAAATGGATTTTAGACC+TGG | - | chr8_2:6178643-6178662 | Msa1230210:intron | 30.0% |
| ATATGAAGTTAACTCTGTTG+AGG | - | chr8_2:6178946-6178965 | Msa1230210:intron | 30.0% | |
| !!! | ACGGTTTTGATTCTATAATG+GGG | + | chr8_2:6179045-6179064 | None:intergenic | 30.0% |
| !! | AAATGGATTTTAGACCTGGA+AGG | - | chr8_2:6178647-6178666 | Msa1230210:intron | 35.0% |
| TTGAGACCATAAGATGTGTA+TGG | + | chr8_2:6178685-6178704 | None:intergenic | 35.0% | |
| CTTTCAGGAGATAAACTAGT+GGG | - | chr8_2:6178851-6178870 | Msa1230210:CDS | 35.0% | |
| !!! | TTTGGTTTTCAGTGACTTCT+AGG | + | chr8_2:6178925-6178944 | None:intergenic | 35.0% |
| ! | TTGATTCTATAATGGGGCTT+TGG | + | chr8_2:6179039-6179058 | None:intergenic | 35.0% |
| TGCTGAAATGCATATGTTTC+AGG | - | chr8_2:6179159-6179178 | Msa1230210:intron | 35.0% | |
| GCTGAAATGCATATGTTTCA+GGG | - | chr8_2:6179160-6179179 | Msa1230210:intron | 35.0% | |
| GAAGTGGCAAGAATTATTTG+TGG | - | chr8_2:6179207-6179226 | Msa1230210:CDS | 35.0% | |
| GATGGACCATACACATCTTA+TGG | - | chr8_2:6178676-6178695 | Msa1230210:intron | 40.0% | |
| ACATTACTCAAAGCAACAGC+TGG | - | chr8_2:6178706-6178725 | Msa1230210:intron | 40.0% | |
| CATTACTCAAAGCAACAGCT+GGG | - | chr8_2:6178707-6178726 | Msa1230210:intron | 40.0% | |
| ! | GAAATTTTGTGACGGCTTTC+AGG | - | chr8_2:6178836-6178855 | Msa1230210:CDS | 40.0% |
| GCTTTCAGGAGATAAACTAG+TGG | - | chr8_2:6178850-6178869 | Msa1230210:CDS | 40.0% | |
| ATGCATATGTTTCAGGGTGA+AGG | - | chr8_2:6179166-6179185 | Msa1230210:intron | 40.0% | |
| TTCAGCAACCACAATCCTAC+TGG | + | chr8_2:6178882-6178901 | None:intergenic | 45.0% | |
| CACTTCTGAGCATCATTTCC+AGG | + | chr8_2:6179193-6179212 | None:intergenic | 45.0% | |
| CTGGAAATGATGCTCAGAAG+TGG | - | chr8_2:6179191-6179210 | Msa1230210:intron | 45.0% | |
| AGACCTGGAAGGTTGACAGA+TGG | - | chr8_2:6178658-6178677 | Msa1230210:intron | 50.0% | |
| TCAAAGCAACAGCTGGGCAA+CGG | - | chr8_2:6178713-6178732 | Msa1230210:intron | 50.0% | |
| CGAGCAGTGCTTATAGGTCA+AGG | - | chr8_2:6178736-6178755 | Msa1230210:intron | 50.0% | |
| ! | AACTAGTGGGTGAAGCCAGT+AGG | - | chr8_2:6178864-6178883 | Msa1230210:CDS | 50.0% |
| ATGTTTCAGGGTGAAGGTCC+TGG | - | chr8_2:6179172-6179191 | Msa1230210:intron | 50.0% | |
| GGTCCATCTGTCAACCTTCC+AGG | + | chr8_2:6178664-6178683 | None:intergenic | 55.0% | |
| CAACGGCGAGCAGTGCTTAT+AGG | - | chr8_2:6178730-6178749 | Msa1230210:intron | 55.0% | |
| GGGTGAAGCCAGTAGGATTG+TGG | - | chr8_2:6178871-6178890 | Msa1230210:CDS | 55.0% | |
| GGTTGCTGAAGCTTGCGTAC+AGG | - | chr8_2:6178892-6178911 | Msa1230210:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 6178552 | 6179246 | 6178552 | ID=Msa1230210;Name=Msa1230210 |
| chr8_2 | mRNA | 6178552 | 6179246 | 6178552 | ID=Msa1230210-mRNA-1;Parent=Msa1230210;Name=Msa1230210-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|0.33|0|1|1|0.75|4|0|107 |
| chr8_2 | exon | 6179197 | 6179246 | 6179197 | ID=Msa1230210-mRNA-1:exon:2238;Parent=Msa1230210-mRNA-1 |
| chr8_2 | exon | 6179041 | 6179138 | 6179041 | ID=Msa1230210-mRNA-1:exon:2237;Parent=Msa1230210-mRNA-1 |
| chr8_2 | exon | 6178831 | 6178940 | 6178831 | ID=Msa1230210-mRNA-1:exon:2236;Parent=Msa1230210-mRNA-1 |
| chr8_2 | exon | 6178552 | 6178617 | 6178552 | ID=Msa1230210-mRNA-1:exon:2235;Parent=Msa1230210-mRNA-1 |
| chr8_2 | CDS | 6179197 | 6179246 | 6179197 | ID=Msa1230210-mRNA-1:cds;Parent=Msa1230210-mRNA-1 |
| chr8_2 | CDS | 6179041 | 6179138 | 6179041 | ID=Msa1230210-mRNA-1:cds;Parent=Msa1230210-mRNA-1 |
| chr8_2 | CDS | 6178831 | 6178940 | 6178831 | ID=Msa1230210-mRNA-1:cds;Parent=Msa1230210-mRNA-1 |
| chr8_2 | CDS | 6178552 | 6178617 | 6178552 | ID=Msa1230210-mRNA-1:cds;Parent=Msa1230210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
