AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1260030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1260030 MtrunA17_Chr4g0061171 100.000 160 0 0 1 160 1 160 2.83e-118 330
Msa1260030 MtrunA17_Chr3g0091481 96.250 160 6 0 1 160 1 160 5.53e-113 317
Msa1260030 MtrunA17_Chr4g0074021 93.711 159 10 0 1 159 1 159 2.73e-109 308
Msa1260030 MtrunA17_Chr5g0418211 91.824 159 13 0 1 159 1 159 9.03e-108 304
Msa1260030 MtrunA17_Chr4g0062241 71.569 102 19 1 1 102 1 92 4.95e-48 150
Msa1260030 MtrunA17_Chr4g0074011 46.094 128 21 2 18 145 42 121 1.23e-25 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1260030 AT1G13950.1 91.195 159 13 1 1 159 1 158 7.23e-104 294
Msa1260030 AT1G69410.1 86.792 159 20 1 1 159 1 158 5.64e-101 287
Msa1260030 AT1G26630.1 81.646 158 28 1 1 158 1 157 3.47e-95 272
Msa1260030 AT1G26630.2 83.465 127 20 1 1 127 1 126 4.40e-76 223

Find 71 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAATGAACGCTAAAATTT+TGG 0.101895 8_2:+61898995 None:intergenic
AGTGGTGTTGGTGGTGTTTA+TGG 0.113621 8_2:-61897183 Msa1260030:three_prime_UTR
TCAAACGAGTATTTCCTTAT+TGG 0.168992 8_2:-61897111 Msa1260030:three_prime_UTR
CATTACATTTGGCAAATTAA+TGG 0.194602 8_2:+61896831 None:intergenic
GTGAAGATGAGTGGTAAATT+AGG 0.229105 8_2:+61898933 None:intergenic
CAAACGAGTATTTCCTTATT+GGG 0.252830 8_2:-61897110 Msa1260030:three_prime_UTR
TTCGTCAGACATATTGATTC+AGG 0.274394 8_2:+61898844 None:intergenic
ACTCATTTCATTCCATAGTT+TGG 0.285322 8_2:-61896902 Msa1260030:three_prime_UTR
CTCATTGACATTTCTGAAGA+TGG 0.299146 8_2:-61898322 Msa1260030:CDS
CAATTTCCCCAACCAAACTA+TGG 0.307891 8_2:+61896890 None:intergenic
CATCGGCCTTGGATTCAAAT+TGG 0.320768 8_2:+61898816 None:intergenic
GTGAGCTTGCTCACTGAAAA+TGG 0.342511 8_2:-61897585 Msa1260030:CDS
TAAAGGACAAATACCCAATA+AGG 0.344877 8_2:+61897097 None:intergenic
TGAAGATGAGTGGTAAATTA+GGG 0.346937 8_2:+61898934 None:intergenic
CTTGGCATGTCCATGCTTCC+CGG 0.359341 8_2:+61898577 None:intergenic
ATCTGTGCTTTGAAGGATAT+TGG 0.363464 8_2:-61897316 Msa1260030:CDS
GGAAGCACCGGCATCGGCCT+TGG 0.374165 8_2:+61898805 None:intergenic
TTTCATTCCATAGTTTGGTT+GGG 0.375187 8_2:-61896897 Msa1260030:three_prime_UTR
AGCTTGTTGAGGATAAGTTT+TGG 0.388597 8_2:+61898784 None:intergenic
TACTATACTTGTAAAGATAA+AGG 0.392946 8_2:+61897080 None:intergenic
ATGAGAACAGAGATTTGTTT+CGG 0.409267 8_2:+61898896 None:intergenic
CGTAAAACTCTGCTCTAGTC+TGG 0.417524 8_2:+61897007 None:intergenic
ATTTCATTCCATAGTTTGGT+TGG 0.427612 8_2:-61896898 Msa1260030:three_prime_UTR
TTCATTCCATAGTTTGGTTG+GGG 0.466367 8_2:-61896896 Msa1260030:three_prime_UTR
GGAGCAGATCTGTGCTTTGA+AGG 0.467522 8_2:-61897323 Msa1260030:CDS
AATGTCAATGAGCTGATAAT+CGG 0.473877 8_2:+61898333 None:intergenic
TAACTATGTATCCATTCTTA+CGG 0.477130 8_2:+61898753 None:intergenic
GCTGGTACCATCCGTAAGAA+TGG 0.484451 8_2:-61898764 Msa1260030:CDS
TTATAATATTCGCAGACCTC+AGG 0.488089 8_2:+61898970 None:intergenic
TGATTCAGGAGTGGCGGTGG+CGG 0.509670 8_2:+61898858 None:intergenic
TGTATCCGTGATGTCTGCTA+TGG 0.510337 8_2:-61897350 Msa1260030:CDS
CTTTGTGTGCAGATCAAGGA+TGG 0.512763 8_2:-61897397 Msa1260030:intron
CACTGAAAATGGAAACACTA+AGG 0.519818 8_2:-61897574 Msa1260030:CDS
TTTGAATCCAAGGCCGATGC+CGG 0.523893 8_2:-61898812 Msa1260030:CDS
TGTGAGACTAGTGGTGTTGG+TGG 0.526439 8_2:-61897192 Msa1260030:three_prime_UTR
GAAGTTTCGACTTCGAAGAC+CGG 0.527896 8_2:-61898596 Msa1260030:CDS
GAATAATCACCTTGCATGGA+CGG 0.531922 8_2:+61898723 None:intergenic
GGATGGATTTGCTGAGGGAA+AGG 0.534900 8_2:-61897380 Msa1260030:CDS
TGTCATCGGTGGGAAGTTTG+AGG 0.539905 8_2:+61897547 None:intergenic
ACTTCGAAGACCGGGAAGCA+TGG 0.555740 8_2:-61898587 Msa1260030:CDS
CTGAGTGAGCAGACTGTCAT+CGG 0.555770 8_2:+61897533 None:intergenic
TATTGATTCAGGAGTGGCGG+TGG 0.557198 8_2:+61898855 None:intergenic
TTACGAATAATCACCTTGCA+TGG 0.560163 8_2:+61898719 None:intergenic
TCTTGTGAGACTAGTGGTGT+TGG 0.565302 8_2:-61897195 Msa1260030:three_prime_UTR
CTGTTCTCATTATAGCTCGT+CGG 0.572672 8_2:-61898883 Msa1260030:five_prime_UTR
AGAGCACCAATTTGAATCCA+AGG 0.579604 8_2:-61898822 Msa1260030:CDS
AATTGCAACAAAATGACACT+TGG 0.580503 8_2:+61898559 None:intergenic
CTTCACTTCAAAGAAGCTTG+AGG 0.580602 8_2:-61898531 Msa1260030:CDS
AAAACTTATCCTCAACAAGC+TGG 0.581192 8_2:-61898782 Msa1260030:CDS
TAGCGTTCATTCCACGCCTG+AGG 0.590265 8_2:-61898986 Msa1260030:five_prime_UTR
AGTGAGCAGACTGTCATCGG+TGG 0.592770 8_2:+61897536 None:intergenic
AAGTTTCGACTTCGAAGACC+GGG 0.594274 8_2:-61898595 Msa1260030:CDS
TGTGCTTTGAAGGATATTGG+TGG 0.594489 8_2:-61897313 Msa1260030:CDS
CGGATGGTACCAGCTTGTTG+AGG 0.595998 8_2:+61898773 None:intergenic
CGTGATGTCTGCTATGGGTG+AGG 0.597852 8_2:-61897344 Msa1260030:CDS
CGATGACAGTCTGCTCACTC+AGG 0.597987 8_2:-61897532 Msa1260030:intron
ATAGATTCTTGTGAGACTAG+TGG 0.603554 8_2:-61897201 Msa1260030:three_prime_UTR
TATGTATCCATTCTTACGGA+TGG 0.607030 8_2:+61898757 None:intergenic
ACATCACAATTATGTGAAGA+CGG 0.610965 8_2:+61898500 None:intergenic
GTATCCGTGATGTCTGCTAT+GGG 0.612684 8_2:-61897349 Msa1260030:CDS
GTGAGCAGACTGTCATCGGT+GGG 0.615967 8_2:+61897537 None:intergenic
GATCAAGGATGGATTTGCTG+AGG 0.618132 8_2:-61897386 Msa1260030:CDS
ATCAAGGATGGATTTGCTGA+GGG 0.621184 8_2:-61897385 Msa1260030:CDS
TTCATGCAATACATAAACTG+TGG 0.634184 8_2:+61896857 None:intergenic
CAGACATCACGGATACAACA+AGG 0.636222 8_2:+61897356 None:intergenic
ACATATTGATTCAGGAGTGG+CGG 0.644758 8_2:+61898852 None:intergenic
CTCACCCATAGCAGACATCA+CGG 0.653023 8_2:+61897345 None:intergenic
ATATTCGCAGACCTCAGGCG+TGG 0.676343 8_2:+61898975 None:intergenic
CAGACATATTGATTCAGGAG+TGG 0.678905 8_2:+61898849 None:intergenic
CAATTGAAAGTGAAGATGAG+TGG 0.711720 8_2:+61898924 None:intergenic
TAATCGGTACGATTCACGTG+AGG 0.748646 8_2:+61898349 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAACAAACTAAATTAAAA+CGG - chr8_2:61898081-61898100 Msa1260030:intron 10.0%
!! TTAAACAAACTAAATTAAAA+CGG - chr8_2:61898081-61898100 Msa1260030:intron 10.0%
!!! CAATTGTTTTTATTTTTGTT+AGG - chr8_2:61897459-61897478 Msa1260030:intron 15.0%
!! TAAACAAACTAAATTAAAAC+GGG - chr8_2:61898082-61898101 Msa1260030:intron 15.0%
!!! CAATTGTTTTTATTTTTGTT+AGG - chr8_2:61897459-61897478 Msa1260030:intron 15.0%
!! TAAACAAACTAAATTAAAAC+GGG - chr8_2:61898082-61898101 Msa1260030:intron 15.0%
!! CACTTAATTAATCAAATCAA+AGG + chr8_2:61897565-61897584 None:intergenic 20.0%
!!! GATTTTGATATTCAATTAGT+TGG - chr8_2:61897642-61897661 Msa1260030:intron 20.0%
!!! GATTTTTTTACTTTCAGATT+AGG - chr8_2:61897700-61897719 Msa1260030:intron 20.0%
!!! TTTAGTTTGTTTAAGCTTTA+TGG + chr8_2:61898075-61898094 None:intergenic 20.0%
!! TTCATAATATAGCTATCAAA+AGG + chr8_2:61898170-61898189 None:intergenic 20.0%
!!! TGATTATGTTTTTGTATTGT+AGG - chr8_2:61898226-61898245 Msa1260030:intron 20.0%
!! TACTATACTTGTAAAGATAA+AGG + chr8_2:61898756-61898775 None:intergenic 20.0%
!! CACTTAATTAATCAAATCAA+AGG + chr8_2:61897565-61897584 None:intergenic 20.0%
!!! GATTTTGATATTCAATTAGT+TGG - chr8_2:61897642-61897661 Msa1260030:intron 20.0%
!!! GATTTTTTTACTTTCAGATT+AGG - chr8_2:61897700-61897719 Msa1260030:intron 20.0%
!!! TTTAGTTTGTTTAAGCTTTA+TGG + chr8_2:61898075-61898094 None:intergenic 20.0%
!! TTCATAATATAGCTATCAAA+AGG + chr8_2:61898170-61898189 None:intergenic 20.0%
!!! TGATTATGTTTTTGTATTGT+AGG - chr8_2:61898226-61898245 Msa1260030:intron 20.0%
!! TACTATACTTGTAAAGATAA+AGG + chr8_2:61898756-61898775 None:intergenic 20.0%
! TAACTATGTATCCATTCTTA+CGG + chr8_2:61897083-61897102 None:intergenic 25.0%
!! TTTTTGTACTTCGTTATTCA+TGG - chr8_2:61897165-61897184 Msa1260030:three_prime_UTR 25.0%
! GTTCATACTTCTTTATAATC+TGG + chr8_2:61897589-61897608 None:intergenic 25.0%
! AAGTATGAACTGATTGTTAT+GGG - chr8_2:61897599-61897618 Msa1260030:intron 25.0%
!! TTAGTTGGAAATTTGTTTGA+AGG - chr8_2:61897657-61897676 Msa1260030:intron 25.0%
! TAATCTGATTTAGATTAGTG+TGG + chr8_2:61897984-61898003 None:intergenic 25.0%
!! AGCTGAGAAAAAAAGATTTT+GGG + chr8_2:61898043-61898062 None:intergenic 25.0%
! TAACTATGTATCCATTCTTA+CGG + chr8_2:61897083-61897102 None:intergenic 25.0%
!! TTTTTGTACTTCGTTATTCA+TGG - chr8_2:61897165-61897184 Msa1260030:three_prime_UTR 25.0%
! GTTCATACTTCTTTATAATC+TGG + chr8_2:61897589-61897608 None:intergenic 25.0%
! AAGTATGAACTGATTGTTAT+GGG - chr8_2:61897599-61897618 Msa1260030:intron 25.0%
!! TTAGTTGGAAATTTGTTTGA+AGG - chr8_2:61897657-61897676 Msa1260030:intron 25.0%
! TAATCTGATTTAGATTAGTG+TGG + chr8_2:61897984-61898003 None:intergenic 25.0%
!! AGCTGAGAAAAAAAGATTTT+GGG + chr8_2:61898043-61898062 None:intergenic 25.0%
TGAAGATGAGTGGTAAATTA+GGG + chr8_2:61896902-61896921 None:intergenic 30.0%
! ATGAGAACAGAGATTTGTTT+CGG + chr8_2:61896940-61896959 None:intergenic 30.0%
!!! TGTTTTTTGTTGTTGCGATT+AGG - chr8_2:61897209-61897228 Msa1260030:three_prime_UTR 30.0%
AATTGCAACAAAATGACACT+TGG + chr8_2:61897277-61897296 None:intergenic 30.0%
ACATCACAATTATGTGAAGA+CGG + chr8_2:61897336-61897355 None:intergenic 30.0%
AATGTCAATGAGCTGATAAT+CGG + chr8_2:61897503-61897522 None:intergenic 30.0%
GAAGTATGAACTGATTGTTA+TGG - chr8_2:61897598-61897617 Msa1260030:intron 30.0%
CCATACACTTCAACATAAAA+GGG + chr8_2:61897912-61897931 None:intergenic 30.0%
CATACACAAAATCAATTCAC+CGG + chr8_2:61897935-61897954 None:intergenic 30.0%
! GAGCTGAGAAAAAAAGATTT+TGG + chr8_2:61898044-61898063 None:intergenic 30.0%
! GGTACAATAACTTTTGTAAC+AGG + chr8_2:61898119-61898138 None:intergenic 30.0%
TAAGCCTGTTACAGATTAAT+GGG + chr8_2:61898141-61898160 None:intergenic 30.0%
ATAAGCCTGTTACAGATTAA+TGG + chr8_2:61898142-61898161 None:intergenic 30.0%
! GATTTGTTCTTGCATTGTAA+TGG - chr8_2:61898332-61898351 Msa1260030:CDS 30.0%
! TGGTGTTTATGGATTTTTGT+TGG - chr8_2:61898661-61898680 Msa1260030:intron 30.0%
TCAAACGAGTATTTCCTTAT+TGG - chr8_2:61898722-61898741 Msa1260030:intron 30.0%
CAAACGAGTATTTCCTTATT+GGG - chr8_2:61898723-61898742 Msa1260030:intron 30.0%
TAAAGGACAAATACCCAATA+AGG + chr8_2:61898739-61898758 None:intergenic 30.0%
ACTCATTTCATTCCATAGTT+TGG - chr8_2:61898931-61898950 Msa1260030:five_prime_UTR 30.0%
ATTTCATTCCATAGTTTGGT+TGG - chr8_2:61898935-61898954 Msa1260030:five_prime_UTR 30.0%
TTTCATTCCATAGTTTGGTT+GGG - chr8_2:61898936-61898955 Msa1260030:five_prime_UTR 30.0%
TTCATGCAATACATAAACTG+TGG + chr8_2:61898979-61898998 None:intergenic 30.0%
! GGAATGAACGCTAAAATTTT+TGG + chr8_2:61896840-61896859 None:intergenic 30.0%
TGAAGATGAGTGGTAAATTA+GGG + chr8_2:61896902-61896921 None:intergenic 30.0%
! ATGAGAACAGAGATTTGTTT+CGG + chr8_2:61896940-61896959 None:intergenic 30.0%
!!! TGTTTTTTGTTGTTGCGATT+AGG - chr8_2:61897209-61897228 Msa1260030:three_prime_UTR 30.0%
AATTGCAACAAAATGACACT+TGG + chr8_2:61897277-61897296 None:intergenic 30.0%
ACATCACAATTATGTGAAGA+CGG + chr8_2:61897336-61897355 None:intergenic 30.0%
AATGTCAATGAGCTGATAAT+CGG + chr8_2:61897503-61897522 None:intergenic 30.0%
GAAGTATGAACTGATTGTTA+TGG - chr8_2:61897598-61897617 Msa1260030:intron 30.0%
CCATACACTTCAACATAAAA+GGG + chr8_2:61897912-61897931 None:intergenic 30.0%
CATACACAAAATCAATTCAC+CGG + chr8_2:61897935-61897954 None:intergenic 30.0%
! GAGCTGAGAAAAAAAGATTT+TGG + chr8_2:61898044-61898063 None:intergenic 30.0%
! GGTACAATAACTTTTGTAAC+AGG + chr8_2:61898119-61898138 None:intergenic 30.0%
TAAGCCTGTTACAGATTAAT+GGG + chr8_2:61898141-61898160 None:intergenic 30.0%
ATAAGCCTGTTACAGATTAA+TGG + chr8_2:61898142-61898161 None:intergenic 30.0%
! GATTTGTTCTTGCATTGTAA+TGG - chr8_2:61898332-61898351 Msa1260030:CDS 30.0%
! TGGTGTTTATGGATTTTTGT+TGG - chr8_2:61898661-61898680 Msa1260030:intron 30.0%
TCAAACGAGTATTTCCTTAT+TGG - chr8_2:61898722-61898741 Msa1260030:intron 30.0%
CAAACGAGTATTTCCTTATT+GGG - chr8_2:61898723-61898742 Msa1260030:intron 30.0%
TAAAGGACAAATACCCAATA+AGG + chr8_2:61898739-61898758 None:intergenic 30.0%
ACTCATTTCATTCCATAGTT+TGG - chr8_2:61898931-61898950 Msa1260030:five_prime_UTR 30.0%
ATTTCATTCCATAGTTTGGT+TGG - chr8_2:61898935-61898954 Msa1260030:five_prime_UTR 30.0%
TTTCATTCCATAGTTTGGTT+GGG - chr8_2:61898936-61898955 Msa1260030:five_prime_UTR 30.0%
TTCATGCAATACATAAACTG+TGG + chr8_2:61898979-61898998 None:intergenic 30.0%
TTATAATATTCGCAGACCTC+AGG + chr8_2:61896866-61896885 None:intergenic 35.0%
GTGAAGATGAGTGGTAAATT+AGG + chr8_2:61896903-61896922 None:intergenic 35.0%
CAATTGAAAGTGAAGATGAG+TGG + chr8_2:61896912-61896931 None:intergenic 35.0%
!! TTCGTCAGACATATTGATTC+AGG + chr8_2:61896992-61897011 None:intergenic 35.0%
!!! AGCTTGTTGAGGATAAGTTT+TGG + chr8_2:61897052-61897071 None:intergenic 35.0%
AAAACTTATCCTCAACAAGC+TGG - chr8_2:61897051-61897070 Msa1260030:three_prime_UTR 35.0%
TATGTATCCATTCTTACGGA+TGG + chr8_2:61897079-61897098 None:intergenic 35.0%
TTACGAATAATCACCTTGCA+TGG + chr8_2:61897117-61897136 None:intergenic 35.0%
! CTCATTGACATTTCTGAAGA+TGG - chr8_2:61897511-61897530 Msa1260030:intron 35.0%
! GTTGGAAATTTGTTTGAAGG+AGG - chr8_2:61897660-61897679 Msa1260030:intron 35.0%
! CCCTTTTATGTTGAAGTGTA+TGG - chr8_2:61897909-61897928 Msa1260030:intron 35.0%
GCCATACACTTCAACATAAA+AGG + chr8_2:61897913-61897932 None:intergenic 35.0%
TTTATGTTGAAGTGTATGGC+CGG - chr8_2:61897913-61897932 Msa1260030:intron 35.0%
AAGCCTGTTACAGATTAATG+GGG + chr8_2:61898140-61898159 None:intergenic 35.0%
CACTGAAAATGGAAACACTA+AGG - chr8_2:61898259-61898278 Msa1260030:intron 35.0%
!! ATCTGTGCTTTGAAGGATAT+TGG - chr8_2:61898517-61898536 Msa1260030:CDS 35.0%
ATAGATTCTTGTGAGACTAG+TGG - chr8_2:61898632-61898651 Msa1260030:intron 35.0%
TTCATTCCATAGTTTGGTTG+GGG - chr8_2:61898937-61898956 Msa1260030:five_prime_UTR 35.0%
TTATAATATTCGCAGACCTC+AGG + chr8_2:61896866-61896885 None:intergenic 35.0%
GTGAAGATGAGTGGTAAATT+AGG + chr8_2:61896903-61896922 None:intergenic 35.0%
CAATTGAAAGTGAAGATGAG+TGG + chr8_2:61896912-61896931 None:intergenic 35.0%
!! TTCGTCAGACATATTGATTC+AGG + chr8_2:61896992-61897011 None:intergenic 35.0%
!!! AGCTTGTTGAGGATAAGTTT+TGG + chr8_2:61897052-61897071 None:intergenic 35.0%
AAAACTTATCCTCAACAAGC+TGG - chr8_2:61897051-61897070 Msa1260030:three_prime_UTR 35.0%
TATGTATCCATTCTTACGGA+TGG + chr8_2:61897079-61897098 None:intergenic 35.0%
TTACGAATAATCACCTTGCA+TGG + chr8_2:61897117-61897136 None:intergenic 35.0%
! CTCATTGACATTTCTGAAGA+TGG - chr8_2:61897511-61897530 Msa1260030:intron 35.0%
! GTTGGAAATTTGTTTGAAGG+AGG - chr8_2:61897660-61897679 Msa1260030:intron 35.0%
! CCCTTTTATGTTGAAGTGTA+TGG - chr8_2:61897909-61897928 Msa1260030:intron 35.0%
GCCATACACTTCAACATAAA+AGG + chr8_2:61897913-61897932 None:intergenic 35.0%
TTTATGTTGAAGTGTATGGC+CGG - chr8_2:61897913-61897932 Msa1260030:intron 35.0%
AAGCCTGTTACAGATTAATG+GGG + chr8_2:61898140-61898159 None:intergenic 35.0%
CACTGAAAATGGAAACACTA+AGG - chr8_2:61898259-61898278 Msa1260030:intron 35.0%
!! ATCTGTGCTTTGAAGGATAT+TGG - chr8_2:61898517-61898536 Msa1260030:CDS 35.0%
ATAGATTCTTGTGAGACTAG+TGG - chr8_2:61898632-61898651 Msa1260030:intron 35.0%
TTCATTCCATAGTTTGGTTG+GGG - chr8_2:61898937-61898956 Msa1260030:five_prime_UTR 35.0%
CTGTTCTCATTATAGCTCGT+CGG - chr8_2:61896950-61896969 Msa1260030:three_prime_UTR 40.0%
!! ACATATTGATTCAGGAGTGG+CGG + chr8_2:61896984-61897003 None:intergenic 40.0%
!! CAGACATATTGATTCAGGAG+TGG + chr8_2:61896987-61897006 None:intergenic 40.0%
! AGAGCACCAATTTGAATCCA+AGG - chr8_2:61897011-61897030 Msa1260030:three_prime_UTR 40.0%
!!! AGGATAAGTTTTGGAAGCAC+CGG + chr8_2:61897043-61897062 None:intergenic 40.0%
TATCAAAAACCGTCCATGCA+AGG - chr8_2:61897101-61897120 Msa1260030:three_prime_UTR 40.0%
GAATAATCACCTTGCATGGA+CGG + chr8_2:61897113-61897132 None:intergenic 40.0%
CTTCACTTCAAAGAAGCTTG+AGG - chr8_2:61897302-61897321 Msa1260030:exon 40.0%
GTACCCCATTAATCTGTAAC+AGG - chr8_2:61898134-61898153 Msa1260030:intron 40.0%
TTTGCTTTGTGTGCAGATCA+AGG - chr8_2:61898432-61898451 Msa1260030:intron 40.0%
!! ATCAAGGATGGATTTGCTGA+GGG - chr8_2:61898448-61898467 Msa1260030:intron 40.0%
!! TGTGCTTTGAAGGATATTGG+TGG - chr8_2:61898520-61898539 Msa1260030:CDS 40.0%
CAATTTCCCCAACCAAACTA+TGG + chr8_2:61898946-61898965 None:intergenic 40.0%
CTGTTCTCATTATAGCTCGT+CGG - chr8_2:61896950-61896969 Msa1260030:three_prime_UTR 40.0%
!! ACATATTGATTCAGGAGTGG+CGG + chr8_2:61896984-61897003 None:intergenic 40.0%
!! CAGACATATTGATTCAGGAG+TGG + chr8_2:61896987-61897006 None:intergenic 40.0%
! AGAGCACCAATTTGAATCCA+AGG - chr8_2:61897011-61897030 Msa1260030:three_prime_UTR 40.0%
!!! AGGATAAGTTTTGGAAGCAC+CGG + chr8_2:61897043-61897062 None:intergenic 40.0%
TATCAAAAACCGTCCATGCA+AGG - chr8_2:61897101-61897120 Msa1260030:three_prime_UTR 40.0%
GAATAATCACCTTGCATGGA+CGG + chr8_2:61897113-61897132 None:intergenic 40.0%
CTTCACTTCAAAGAAGCTTG+AGG - chr8_2:61897302-61897321 Msa1260030:exon 40.0%
GTACCCCATTAATCTGTAAC+AGG - chr8_2:61898134-61898153 Msa1260030:intron 40.0%
TTTGCTTTGTGTGCAGATCA+AGG - chr8_2:61898432-61898451 Msa1260030:intron 40.0%
!! ATCAAGGATGGATTTGCTGA+GGG - chr8_2:61898448-61898467 Msa1260030:intron 40.0%
!! TGTGCTTTGAAGGATATTGG+TGG - chr8_2:61898520-61898539 Msa1260030:CDS 40.0%
CAATTTCCCCAACCAAACTA+TGG + chr8_2:61898946-61898965 None:intergenic 40.0%
CATCGGCCTTGGATTCAAAT+TGG + chr8_2:61897020-61897039 None:intergenic 45.0%
GAAGTTTCGACTTCGAAGAC+CGG - chr8_2:61897237-61897256 Msa1260030:three_prime_UTR 45.0%
AAGTTTCGACTTCGAAGACC+GGG - chr8_2:61897238-61897257 Msa1260030:three_prime_UTR 45.0%
TAATCGGTACGATTCACGTG+AGG + chr8_2:61897487-61897506 None:intergenic 45.0%
! GTGAGCTTGCTCACTGAAAA+TGG - chr8_2:61898248-61898267 Msa1260030:intron 45.0%
CTTTGTGTGCAGATCAAGGA+TGG - chr8_2:61898436-61898455 Msa1260030:intron 45.0%
!! GATCAAGGATGGATTTGCTG+AGG - chr8_2:61898447-61898466 Msa1260030:intron 45.0%
CAGACATCACGGATACAACA+AGG + chr8_2:61898480-61898499 None:intergenic 45.0%
TGTATCCGTGATGTCTGCTA+TGG - chr8_2:61898483-61898502 Msa1260030:intron 45.0%
GTATCCGTGATGTCTGCTAT+GGG - chr8_2:61898484-61898503 Msa1260030:intron 45.0%
TCTTGTGAGACTAGTGGTGT+TGG - chr8_2:61898638-61898657 Msa1260030:intron 45.0%
!! AGTGGTGTTGGTGGTGTTTA+TGG - chr8_2:61898650-61898669 Msa1260030:intron 45.0%
CGTAAAACTCTGCTCTAGTC+TGG + chr8_2:61898829-61898848 None:intergenic 45.0%
CATCGGCCTTGGATTCAAAT+TGG + chr8_2:61897020-61897039 None:intergenic 45.0%
GAAGTTTCGACTTCGAAGAC+CGG - chr8_2:61897237-61897256 Msa1260030:three_prime_UTR 45.0%
AAGTTTCGACTTCGAAGACC+GGG - chr8_2:61897238-61897257 Msa1260030:three_prime_UTR 45.0%
TAATCGGTACGATTCACGTG+AGG + chr8_2:61897487-61897506 None:intergenic 45.0%
! GTGAGCTTGCTCACTGAAAA+TGG - chr8_2:61898248-61898267 Msa1260030:intron 45.0%
CTTTGTGTGCAGATCAAGGA+TGG - chr8_2:61898436-61898455 Msa1260030:intron 45.0%
!! GATCAAGGATGGATTTGCTG+AGG - chr8_2:61898447-61898466 Msa1260030:intron 45.0%
CAGACATCACGGATACAACA+AGG + chr8_2:61898480-61898499 None:intergenic 45.0%
TGTATCCGTGATGTCTGCTA+TGG - chr8_2:61898483-61898502 Msa1260030:intron 45.0%
GTATCCGTGATGTCTGCTAT+GGG - chr8_2:61898484-61898503 Msa1260030:intron 45.0%
TCTTGTGAGACTAGTGGTGT+TGG - chr8_2:61898638-61898657 Msa1260030:intron 45.0%
!! AGTGGTGTTGGTGGTGTTTA+TGG - chr8_2:61898650-61898669 Msa1260030:intron 45.0%
CGTAAAACTCTGCTCTAGTC+TGG + chr8_2:61898829-61898848 None:intergenic 45.0%
!! TATTGATTCAGGAGTGGCGG+TGG + chr8_2:61896981-61897000 None:intergenic 50.0%
TTTGAATCCAAGGCCGATGC+CGG - chr8_2:61897021-61897040 Msa1260030:three_prime_UTR 50.0%
!!! AGTTTTGGAAGCACCGGCAT+CGG + chr8_2:61897037-61897056 None:intergenic 50.0%
GCTGGTACCATCCGTAAGAA+TGG - chr8_2:61897069-61897088 Msa1260030:three_prime_UTR 50.0%
TGTCATCGGTGGGAAGTTTG+AGG + chr8_2:61898289-61898308 None:intergenic 50.0%
CTGAGTGAGCAGACTGTCAT+CGG + chr8_2:61898303-61898322 None:intergenic 50.0%
!! GGATGGATTTGCTGAGGGAA+AGG - chr8_2:61898453-61898472 Msa1260030:intron 50.0%
CTCACCCATAGCAGACATCA+CGG + chr8_2:61898491-61898510 None:intergenic 50.0%
GGAGCAGATCTGTGCTTTGA+AGG - chr8_2:61898510-61898529 Msa1260030:CDS 50.0%
! TGTGAGACTAGTGGTGTTGG+TGG - chr8_2:61898641-61898660 Msa1260030:intron 50.0%
!! TATTGATTCAGGAGTGGCGG+TGG + chr8_2:61896981-61897000 None:intergenic 50.0%
TTTGAATCCAAGGCCGATGC+CGG - chr8_2:61897021-61897040 Msa1260030:three_prime_UTR 50.0%
!!! AGTTTTGGAAGCACCGGCAT+CGG + chr8_2:61897037-61897056 None:intergenic 50.0%
GCTGGTACCATCCGTAAGAA+TGG - chr8_2:61897069-61897088 Msa1260030:three_prime_UTR 50.0%
TGTCATCGGTGGGAAGTTTG+AGG + chr8_2:61898289-61898308 None:intergenic 50.0%
CTGAGTGAGCAGACTGTCAT+CGG + chr8_2:61898303-61898322 None:intergenic 50.0%
!! GGATGGATTTGCTGAGGGAA+AGG - chr8_2:61898453-61898472 Msa1260030:intron 50.0%
CTCACCCATAGCAGACATCA+CGG + chr8_2:61898491-61898510 None:intergenic 50.0%
GGAGCAGATCTGTGCTTTGA+AGG - chr8_2:61898510-61898529 Msa1260030:CDS 50.0%
! TGTGAGACTAGTGGTGTTGG+TGG - chr8_2:61898641-61898660 Msa1260030:intron 50.0%
TAGCGTTCATTCCACGCCTG+AGG - chr8_2:61896847-61896866 Msa1260030:three_prime_UTR 55.0%
ATATTCGCAGACCTCAGGCG+TGG + chr8_2:61896861-61896880 None:intergenic 55.0%
!! CGGATGGTACCAGCTTGTTG+AGG + chr8_2:61897063-61897082 None:intergenic 55.0%
ACTTCGAAGACCGGGAAGCA+TGG - chr8_2:61897246-61897265 Msa1260030:three_prime_UTR 55.0%
CTTGGCATGTCCATGCTTCC+CGG + chr8_2:61897259-61897278 None:intergenic 55.0%
GTGAGCAGACTGTCATCGGT+GGG + chr8_2:61898299-61898318 None:intergenic 55.0%
AGTGAGCAGACTGTCATCGG+TGG + chr8_2:61898300-61898319 None:intergenic 55.0%
CGATGACAGTCTGCTCACTC+AGG - chr8_2:61898301-61898320 Msa1260030:intron 55.0%
CGTGATGTCTGCTATGGGTG+AGG - chr8_2:61898489-61898508 Msa1260030:intron 55.0%
TAGCGTTCATTCCACGCCTG+AGG - chr8_2:61896847-61896866 Msa1260030:three_prime_UTR 55.0%
ATATTCGCAGACCTCAGGCG+TGG + chr8_2:61896861-61896880 None:intergenic 55.0%
!! CGGATGGTACCAGCTTGTTG+AGG + chr8_2:61897063-61897082 None:intergenic 55.0%
ACTTCGAAGACCGGGAAGCA+TGG - chr8_2:61897246-61897265 Msa1260030:three_prime_UTR 55.0%
CTTGGCATGTCCATGCTTCC+CGG + chr8_2:61897259-61897278 None:intergenic 55.0%
GTGAGCAGACTGTCATCGGT+GGG + chr8_2:61898299-61898318 None:intergenic 55.0%
AGTGAGCAGACTGTCATCGG+TGG + chr8_2:61898300-61898319 None:intergenic 55.0%
CGATGACAGTCTGCTCACTC+AGG - chr8_2:61898301-61898320 Msa1260030:intron 55.0%
CGTGATGTCTGCTATGGGTG+AGG - chr8_2:61898489-61898508 Msa1260030:intron 55.0%
TGATTCAGGAGTGGCGGTGG+CGG + chr8_2:61896978-61896997 None:intergenic 60.0%
TGATTCAGGAGTGGCGGTGG+CGG + chr8_2:61896978-61896997 None:intergenic 60.0%
!! GGAAGCACCGGCATCGGCCT+TGG + chr8_2:61897031-61897050 None:intergenic 70.0%
!! GGAAGCACCGGCATCGGCCT+TGG + chr8_2:61897031-61897050 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 61896840 61899015 61896840 ID=Msa1260030;Name=Msa1260030
chr8_2 mRNA 61896840 61899015 61896840 ID=Msa1260030-mRNA-1;Parent=Msa1260030;Name=Msa1260030-mRNA-1;_AED=0.11;_eAED=0.11;_QI=160|1|1|1|1|1|5|463|160
chr8_2 exon 61896840 61897407 61896840 ID=Msa1260030-mRNA-1:exon:19581;Parent=Msa1260030-mRNA-1
chr8_2 exon 61897533 61897607 61897533 ID=Msa1260030-mRNA-1:exon:19580;Parent=Msa1260030-mRNA-1
chr8_2 exon 61898318 61898374 61898318 ID=Msa1260030-mRNA-1:exon:19579;Parent=Msa1260030-mRNA-1
chr8_2 exon 61898502 61898624 61898502 ID=Msa1260030-mRNA-1:exon:19578;Parent=Msa1260030-mRNA-1
chr8_2 exon 61898733 61899015 61898733 ID=Msa1260030-mRNA-1:exon:19577;Parent=Msa1260030-mRNA-1
chr8_2 five_prime_UTR 61898856 61899015 61898856 ID=Msa1260030-mRNA-1:five_prime_utr;Parent=Msa1260030-mRNA-1
chr8_2 CDS 61898733 61898855 61898733 ID=Msa1260030-mRNA-1:cds;Parent=Msa1260030-mRNA-1
chr8_2 CDS 61898502 61898624 61898502 ID=Msa1260030-mRNA-1:cds;Parent=Msa1260030-mRNA-1
chr8_2 CDS 61898318 61898374 61898318 ID=Msa1260030-mRNA-1:cds;Parent=Msa1260030-mRNA-1
chr8_2 CDS 61897533 61897607 61897533 ID=Msa1260030-mRNA-1:cds;Parent=Msa1260030-mRNA-1
chr8_2 CDS 61897303 61897407 61897303 ID=Msa1260030-mRNA-1:cds;Parent=Msa1260030-mRNA-1
chr8_2 three_prime_UTR 61896840 61897302 61896840 ID=Msa1260030-mRNA-1:three_prime_utr;Parent=Msa1260030-mRNA-1
Gene Sequence

>Msa1260030

ATGTCTGACGAAGAGCACCAATTTGAATCCAAGGCCGATGCCGGTGCTTCCAAAACTTATCCTCAACAAGCTGGTACCATCCGTAAGAATGGATACATAGTTATCAAAAACCGTCCATGCAAGGTTGTTGAAGTTTCGACTTCGAAGACCGGGAAGCATGGACATGCCAAGTGTCATTTTGTTGCAATTGATATCTTCACTTCAAAGAAGCTTGAGGATATCGTGCCGTCTTCACATAATTGTGATGTTCCTCACGTGAATCGTACCGATTATCAGCTCATTGACATTTCTGAAGATGGATTTGTGAGCTTGCTCACTGAAAATGGAAACACTAAGGATGACCTCAAACTTCCCACCGATGACAGTCTGCTCACTCAGATCAAGGATGGATTTGCTGAGGGAAAGGACCTTGTTGTATCCGTGATGTCTGCTATGGGTGAGGAGCAGATCTGTGCTTTGAAGGATATTGGTGGAAAGAACTAA
Protein sequence

>Msa1260030

MSDEEHQFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTDDSLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGGKN*