AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1260630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1260630 MtrunA17_Chr4g0062111 97.175 177 5 0 1 177 1 177 2.53e-124 350
Msa1260630 MtrunA17_Chr8g0372221 40.940 149 81 4 33 177 61 206 9.88e-30 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1260630 AT5G23040.2 77.852 149 30 2 32 177 36 184 1.56e-80 239
Msa1260630 AT5G23040.1 77.852 149 30 2 32 177 36 184 1.56e-80 239
Msa1260630 AT3G51140.2 43.548 124 68 2 54 177 83 204 1.12e-29 108
Msa1260630 AT3G51140.1 38.509 161 93 3 17 177 50 204 6.23e-29 108

Find 61 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTAACTCTGGGGAATTT+TGG 0.086811 8_2:+62672560 None:intergenic
CAATAACATGCTCTTGAATT+AGG 0.126696 8_2:-62674353 Msa1260630:five_prime_UTR
ATGCTTCTGAAGAAGAAATT+TGG 0.165582 8_2:-62672502 Msa1260630:CDS
GAGGAAAGCGGTGGCGAAAT+TGG 0.249902 8_2:+62674254 None:intergenic
ATTGTTTCTCTTTGCCTTCA+TGG 0.298571 8_2:-62672255 Msa1260630:CDS
AATAACATGCTCTTGAATTA+GGG 0.304047 8_2:-62674352 Msa1260630:five_prime_UTR
TGCTTCTGAAGAAGAAATTT+GGG 0.335100 8_2:-62672501 Msa1260630:CDS
TTGGATTCTGCAGGCCCCTC+CGG 0.343652 8_2:-62674129 Msa1260630:intron
AATTCCCCAGAGTTAGTGTT+TGG 0.356525 8_2:-62672556 Msa1260630:CDS
GGAACGCGTTCGTTTCGTTT+TGG 0.380045 8_2:+62674390 None:intergenic
ATTATGAATTCTGCTGAAAC+TGG 0.386488 8_2:-62672221 Msa1260630:CDS
TCTTTGCCTTCATGGGTGGC+TGG 0.389646 8_2:-62672247 Msa1260630:CDS
TTGTTTCTCTTTGCCTTCAT+GGG 0.395534 8_2:-62672254 Msa1260630:CDS
AGGAAAGCGGTGGCGAAATT+GGG 0.406392 8_2:+62674255 None:intergenic
CATGCTCTTGAATTAGGGAT+TGG 0.418376 8_2:-62674347 Msa1260630:five_prime_UTR
TGGATTCTGCAGGCCCCTCC+GGG 0.429772 8_2:-62674128 Msa1260630:intron
ATTCCCCAGAGTTAGTGTTT+GGG 0.449394 8_2:-62672555 Msa1260630:CDS
GAAACTTGGCGAAACGTTGT+GGG 0.450485 8_2:+62674091 None:intergenic
CACACGGCGCATTCTAGCAT+TGG 0.454284 8_2:-62674477 Msa1260630:five_prime_UTR
ATGCTCTTGAATTAGGGATT+GGG 0.458692 8_2:-62674346 Msa1260630:five_prime_UTR
GTTATTGAACTCGCCGGAAA+AGG 0.462443 8_2:+62674369 None:intergenic
TGGGATCCTTACCGACGCCT+TGG 0.464510 8_2:-62672536 Msa1260630:CDS
AGGATCCCAAACACTAACTC+TGG 0.471905 8_2:+62672550 None:intergenic
CTGAGGATAATTTCAGTTGG+TGG 0.473276 8_2:+62672281 None:intergenic
TTTCTGTTGCAACAGTATGC+TGG 0.473842 8_2:-62672467 Msa1260630:CDS
ACTTAGTACGAAACGAAACT+TGG 0.478032 8_2:+62674077 None:intergenic
CGAAACTTGGCGAAACGTTG+TGG 0.490078 8_2:+62674090 None:intergenic
TCAAATCAAACCTGAAAAGC+AGG 0.503028 8_2:+62672197 None:intergenic
TGCGCTGTAGACACACCATA+TGG 0.511360 8_2:-62674055 Msa1260630:CDS
ACATAGGTGATACCTCCATA+TGG 0.517967 8_2:+62674040 None:intergenic
TTTCTTCTTCAGAAGCATCA+TGG 0.518629 8_2:+62672507 None:intergenic
TTTCTCTTTGCCTTCATGGG+TGG 0.529189 8_2:-62672251 Msa1260630:CDS
GGATCCCAAACACTAACTCT+GGG 0.535496 8_2:+62672551 None:intergenic
GGCCCCTCCGGGAAAATACA+AGG 0.538659 8_2:-62674117 Msa1260630:CDS
AATGCTCCAGCCACCCATGA+AGG 0.539964 8_2:+62672241 None:intergenic
TTGAGAGAGTGAATTGCAGA+AGG 0.548026 8_2:+62674315 None:intergenic
ATAATCACGTAATCTGCTAT+CGG 0.549468 8_2:-62674435 Msa1260630:five_prime_UTR
CTTCTGAGGATAATTTCAGT+TGG 0.554844 8_2:+62672278 None:intergenic
AGCACATTCTTGACCCATGG+TGG 0.555534 8_2:+62672320 None:intergenic
CACATTCTTGACCCATGGTG+GGG 0.558838 8_2:+62672322 None:intergenic
TTTAGCACATTCTTGACCCA+TGG 0.565764 8_2:+62672317 None:intergenic
CTAACACCAAGGCGTCGGTA+AGG 0.567021 8_2:+62672530 None:intergenic
GAGGAGCGTAGAATCAATAG+AGG 0.575040 8_2:-62672438 Msa1260630:CDS
GCACATTCTTGACCCATGGT+GGG 0.578738 8_2:+62672321 None:intergenic
GAGCATGTTATTGAACTCGC+CGG 0.581075 8_2:+62674363 None:intergenic
TGATGGCTAGTTTCGTACAA+AGG 0.587486 8_2:-62672397 Msa1260630:CDS
ACGGCGCATTCTAGCATTGG+AGG 0.600973 8_2:-62674474 Msa1260630:five_prime_UTR
AGTTGAGGAGTCCCCACCAT+GGG 0.606304 8_2:-62672333 Msa1260630:CDS
CAAGTTGAAGAAGAAAGTTG+AGG 0.611137 8_2:-62672348 Msa1260630:CDS
CAAAGAGAAACAATCTTCTG+AGG 0.616936 8_2:+62672264 None:intergenic
CATGGCTAACACCAAGGCGT+CGG 0.618805 8_2:+62672525 None:intergenic
TGGCTAGTTTCGTACAAAGG+AGG 0.635562 8_2:-62672394 Msa1260630:CDS
AACAGTATGCTGGACATGAG+AGG 0.646098 8_2:-62672457 Msa1260630:CDS
GCTTCTGAAGAAGAAATTTG+GGG 0.649344 8_2:-62672500 Msa1260630:CDS
AAGCATCATGGCTAACACCA+AGG 0.688521 8_2:+62672519 None:intergenic
AAGTTGAGGAGTCCCCACCA+TGG 0.688946 8_2:-62672334 Msa1260630:CDS
TCTCAATTACAACAACAACA+TGG 0.695771 8_2:-62674298 Msa1260630:exon
CTTCTGAAGAAGAAATTTGG+GGG 0.702966 8_2:-62672499 Msa1260630:CDS
GATGTGTGGTAGTTTGCACA+CGG 0.704273 8_2:-62674493 Msa1260630:five_prime_UTR
GCTGTAGACACACCATATGG+AGG 0.724697 8_2:-62674052 Msa1260630:intron
GATCCCAAACACTAACTCTG+GGG 0.736381 8_2:+62672552 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTTATTTATTCCATA+AGG + chr8_2:62673939-62673958 None:intergenic 10.0%
!! AATAAATGAATTAAGGATAT+AGG + chr8_2:62672355-62672374 None:intergenic 15.0%
!! GAAAACTAAATAATATAGAT+AGG + chr8_2:62672653-62672672 None:intergenic 15.0%
!! TAAAAATAACGATAAATTCT+AGG + chr8_2:62673282-62673301 None:intergenic 15.0%
!!! TCCATATTAATTAATGCTTT+TGG - chr8_2:62672409-62672428 Msa1260630:CDS 20.0%
!!! TCCAAAAGCATTAATTAATA+TGG + chr8_2:62672413-62672432 None:intergenic 20.0%
!! TAAATTCGAAGAAAAAACAT+AGG + chr8_2:62672536-62672555 None:intergenic 20.0%
!! TAAAATTCCACATATAAATG+CGG + chr8_2:62673026-62673045 None:intergenic 20.0%
!! CATGTATTATGAAATGTTTA+GGG + chr8_2:62673322-62673341 None:intergenic 20.0%
!!! AAATTGCAATCCTAATTTTT+TGG + chr8_2:62673550-62673569 None:intergenic 20.0%
!! AATTTATTTATTCCATAAGG+TGG + chr8_2:62673936-62673955 None:intergenic 20.0%
!!! TGCTTTTGAAAAAATATTGA+TGG - chr8_2:62674143-62674162 Msa1260630:intron 20.0%
! AATAACATGCTCTTGAATTA+GGG - chr8_2:62672205-62672224 Msa1260630:CDS 25.0%
! AAATGAATTAAGGATATAGG+AGG + chr8_2:62672352-62672371 None:intergenic 25.0%
! TGAAGCGAATAAATGAATTA+AGG + chr8_2:62672362-62672381 None:intergenic 25.0%
! TTTATTTGACTATTGCTACT+TGG - chr8_2:62672552-62672571 Msa1260630:CDS 25.0%
! AATATAGATAGGTGAATATG+TGG + chr8_2:62672642-62672661 None:intergenic 25.0%
!!! AACTTTTGTTTAAAATGCAC+CGG - chr8_2:62672748-62672767 Msa1260630:intron 25.0%
! TTGTTGTAAGCAAATAGTAA+GGG - chr8_2:62672786-62672805 Msa1260630:intron 25.0%
! AAAATTCCACATATAAATGC+GGG + chr8_2:62673025-62673044 None:intergenic 25.0%
! TAATCTATATTATACGCCAA+AGG + chr8_2:62673204-62673223 None:intergenic 25.0%
! GCATGTATTATGAAATGTTT+AGG + chr8_2:62673323-62673342 None:intergenic 25.0%
! TAGAAGCAACATCAATTATA+AGG - chr8_2:62673459-62673478 Msa1260630:intron 25.0%
! AACATCAATTATAAGGATAG+AGG - chr8_2:62673466-62673485 Msa1260630:intron 25.0%
! ACATCAATTATAAGGATAGA+GGG - chr8_2:62673467-62673486 Msa1260630:intron 25.0%
! TATAAAAGTTCCATACAACA+GGG - chr8_2:62673507-62673526 Msa1260630:intron 25.0%
!!! GTAGATCTGAAGTTTTTTAT+TGG + chr8_2:62673724-62673743 None:intergenic 25.0%
! CAAAAATCATCTTAAAGCTA+AGG + chr8_2:62673824-62673843 None:intergenic 25.0%
!!! CTTAGCTTTAAGATGATTTT+TGG - chr8_2:62673822-62673841 Msa1260630:intron 25.0%
ATAATCACGTAATCTGCTAT+CGG - chr8_2:62672122-62672141 Msa1260630:three_prime_UTR 30.0%
CAATAACATGCTCTTGAATT+AGG - chr8_2:62672204-62672223 Msa1260630:CDS 30.0%
TCTCAATTACAACAACAACA+TGG - chr8_2:62672259-62672278 Msa1260630:CDS 30.0%
ACAAAATGTCTTATCCCATT+AGG - chr8_2:62672685-62672704 Msa1260630:intron 30.0%
TCAACGGAATAAAACCTAAT+GGG + chr8_2:62672702-62672721 None:intergenic 30.0%
TTCAACGGAATAAAACCTAA+TGG + chr8_2:62672703-62672722 None:intergenic 30.0%
! CTTGTTGTAAGCAAATAGTA+AGG - chr8_2:62672785-62672804 Msa1260630:intron 30.0%
! CAAATAGTAAGGGTTTGTTT+GGG - chr8_2:62672796-62672815 Msa1260630:intron 30.0%
TATTATACGCCAAAGGTTAA+GGG + chr8_2:62673197-62673216 None:intergenic 30.0%
ATATTATACGCCAAAGGTTA+AGG + chr8_2:62673198-62673217 None:intergenic 30.0%
TGGCGTATAATATAGATTAG+TGG - chr8_2:62673205-62673224 Msa1260630:intron 30.0%
!!! TATGCCTGTAGGTTATTTTT+TGG - chr8_2:62673369-62673388 Msa1260630:intron 30.0%
!!! ATGCCTGTAGGTTATTTTTT+GGG - chr8_2:62673370-62673389 Msa1260630:intron 30.0%
CTATAAAAGTTCCATACAAC+AGG - chr8_2:62673506-62673525 Msa1260630:intron 30.0%
!! TTTCAATCTCCGATTTTAGT+TGG - chr8_2:62673567-62673586 Msa1260630:intron 30.0%
!! GAACTTGATCTTTGATTTGA+TGG - chr8_2:62673615-62673634 Msa1260630:intron 30.0%
CAGCATTTATATTCCTTTCT+TGG - chr8_2:62673681-62673700 Msa1260630:intron 30.0%
AAAAGATTCTCAACCAAGAA+AGG + chr8_2:62673697-62673716 None:intergenic 30.0%
! ATGCTTCTGAAGAAGAAATT+TGG - chr8_2:62674055-62674074 Msa1260630:CDS 30.0%
! TGCTTCTGAAGAAGAAATTT+GGG - chr8_2:62674056-62674075 Msa1260630:CDS 30.0%
ATTATGAATTCTGCTGAAAC+TGG - chr8_2:62674336-62674355 Msa1260630:five_prime_UTR 30.0%
ATGCTCTTGAATTAGGGATT+GGG - chr8_2:62672211-62672230 Msa1260630:CDS 35.0%
!!! GAAGAAGGAGTACAGTTTTT+TGG + chr8_2:62672330-62672349 None:intergenic 35.0%
TTAAGGATATAGGAGGAAGA+AGG + chr8_2:62672345-62672364 None:intergenic 35.0%
! TTAATGCTTTTGGATTCTGC+AGG - chr8_2:62672419-62672438 Msa1260630:CDS 35.0%
ACTTAGTACGAAACGAAACT+TGG + chr8_2:62672483-62672502 None:intergenic 35.0%
ACAAGTGCAAAACTAAAAGC+CGG + chr8_2:62672770-62672789 None:intergenic 35.0%
! GCAAATAGTAAGGGTTTGTT+TGG - chr8_2:62672795-62672814 Msa1260630:intron 35.0%
! AAGGGTTTGTTTGGGAATTT+AGG - chr8_2:62672804-62672823 Msa1260630:intron 35.0%
GTTTGTTTGGGAATTTAGGA+GGG - chr8_2:62672808-62672827 Msa1260630:intron 35.0%
TTTGTTTGGGAATTTAGGAG+GGG - chr8_2:62672809-62672828 Msa1260630:intron 35.0%
!!! TGCCTGTAGGTTATTTTTTG+GGG - chr8_2:62673371-62673390 Msa1260630:intron 35.0%
!!! TGTAGGTTATTTTTTGGGGA+GGG - chr8_2:62673375-62673394 Msa1260630:intron 35.0%
CAATTATAAGGATAGAGGGA+AGG - chr8_2:62673471-62673490 Msa1260630:intron 35.0%
!!! TGCAATCCTAATTTTTTGGC+TGG + chr8_2:62673546-62673565 None:intergenic 35.0%
! TTGATGGTCATAGTAATGAG+TGG - chr8_2:62673631-62673650 Msa1260630:intron 35.0%
!!! ACTAGTTTTATGTCCTCTTC+TGG - chr8_2:62673773-62673792 Msa1260630:intron 35.0%
! GTCTTCTATTTTCCACCTTA+TGG - chr8_2:62673921-62673940 Msa1260630:intron 35.0%
! ATACGTGCATCCTTTTATAC+AGG - chr8_2:62673967-62673986 Msa1260630:intron 35.0%
TGGGACATTACCTGTATAAA+AGG + chr8_2:62673980-62673999 None:intergenic 35.0%
!! TTTCTTCTTCAGAAGCATCA+TGG + chr8_2:62674053-62674072 None:intergenic 35.0%
! GCTTCTGAAGAAGAAATTTG+GGG - chr8_2:62674057-62674076 Msa1260630:CDS 35.0%
! CTTCTGAAGAAGAAATTTGG+GGG - chr8_2:62674058-62674077 Msa1260630:CDS 35.0%
! CAAGTTGAAGAAGAAAGTTG+AGG - chr8_2:62674209-62674228 Msa1260630:intron 35.0%
! CTTCTGAGGATAATTTCAGT+TGG + chr8_2:62674282-62674301 None:intergenic 35.0%
CAAAGAGAAACAATCTTCTG+AGG + chr8_2:62674296-62674315 None:intergenic 35.0%
! ATTGTTTCTCTTTGCCTTCA+TGG - chr8_2:62674302-62674321 Msa1260630:five_prime_UTR 35.0%
! TTGTTTCTCTTTGCCTTCAT+GGG - chr8_2:62674303-62674322 Msa1260630:five_prime_UTR 35.0%
TCAAATCAAACCTGAAAAGC+AGG + chr8_2:62674363-62674382 None:intergenic 35.0%
!! TTTCGTTTTGGTCTGCAAGA+GGG + chr8_2:62672158-62672177 None:intergenic 40.0%
CATGCTCTTGAATTAGGGAT+TGG - chr8_2:62672210-62672229 Msa1260630:CDS 40.0%
TTGAGAGAGTGAATTGCAGA+AGG + chr8_2:62672245-62672264 None:intergenic 40.0%
!!! CAGTTTTTTGGAGAGGAAAG+CGG + chr8_2:62672318-62672337 None:intergenic 40.0%
!!! AGGAGTACAGTTTTTTGGAG+AGG + chr8_2:62672325-62672344 None:intergenic 40.0%
! ATTCCTTGTATTTTCCCGGA+GGG + chr8_2:62672446-62672465 None:intergenic 40.0%
! TGGGATTCCTTGTATTTTCC+CGG + chr8_2:62672450-62672469 None:intergenic 40.0%
ACATAGGTGATACCTCCATA+TGG + chr8_2:62672520-62672539 None:intergenic 40.0%
!!! GGTTTTATTCCGTTGAATCG+TGG - chr8_2:62672706-62672725 Msa1260630:intron 40.0%
GATACACTTCCACGATTCAA+CGG + chr8_2:62672718-62672737 None:intergenic 40.0%
! GGTTTGTTTGGGAATTTAGG+AGG - chr8_2:62672807-62672826 Msa1260630:intron 40.0%
AGGGGAGGGTTTAAAAACTA+GGG - chr8_2:62672827-62672846 Msa1260630:intron 40.0%
GAGGGTTTAAAAACTAGGGA+AGG - chr8_2:62672831-62672850 Msa1260630:intron 40.0%
GGTTTAAAAACTAGGGAAGG+AGG - chr8_2:62672834-62672853 Msa1260630:intron 40.0%
TGCAATCCCGCATTTATATG+TGG - chr8_2:62673016-62673035 Msa1260630:intron 40.0%
AGCAATGATCCCTTAACCTT+TGG - chr8_2:62673185-62673204 Msa1260630:intron 40.0%
TACATGCTGAGAAGCAAACA+AGG - chr8_2:62673336-62673355 Msa1260630:intron 40.0%
GCTTTGATAAGTATGCCTGT+AGG - chr8_2:62673358-62673377 Msa1260630:intron 40.0%
CTCCCCAAAAAATAACCTAC+AGG + chr8_2:62673376-62673395 None:intergenic 40.0%
!!! CTGTAGGTTATTTTTTGGGG+AGG - chr8_2:62673374-62673393 Msa1260630:intron 40.0%
!!! GTAGGTTATTTTTTGGGGAG+GGG - chr8_2:62673376-62673395 Msa1260630:intron 40.0%
!!! TAGGTTATTTTTTGGGGAGG+GGG - chr8_2:62673377-62673396 Msa1260630:intron 40.0%
TCCTGACCAGCCAAAAAATT+AGG - chr8_2:62673537-62673556 Msa1260630:intron 40.0%
!!! TCCTAATTTTTTGGCTGGTC+AGG + chr8_2:62673541-62673560 None:intergenic 40.0%
! CAGTTGGCTCCAACTAAAAT+CGG + chr8_2:62673579-62673598 None:intergenic 40.0%
!! CACTAACTCTGGGGAATTTT+GGG + chr8_2:62673999-62674018 None:intergenic 40.0%
!! ACACTAACTCTGGGGAATTT+TGG + chr8_2:62674000-62674019 None:intergenic 40.0%
AATTCCCCAGAGTTAGTGTT+TGG - chr8_2:62674001-62674020 Msa1260630:intron 40.0%
ATTCCCCAGAGTTAGTGTTT+GGG - chr8_2:62674002-62674021 Msa1260630:intron 40.0%
TTTCTGTTGCAACAGTATGC+TGG - chr8_2:62674090-62674109 Msa1260630:CDS 40.0%
!! TGATGGCTAGTTTCGTACAA+AGG - chr8_2:62674160-62674179 Msa1260630:intron 40.0%
TTTAGCACATTCTTGACCCA+TGG + chr8_2:62674243-62674262 None:intergenic 40.0%
CTGAGGATAATTTCAGTTGG+TGG + chr8_2:62674279-62674298 None:intergenic 40.0%
! GATGTGTGGTAGTTTGCACA+CGG - chr8_2:62672064-62672083 Msa1260630:three_prime_UTR 45.0%
!! GTTTCGTTTTGGTCTGCAAG+AGG + chr8_2:62672159-62672178 None:intergenic 45.0%
! AAACGAACGCGTTCCTTTTC+CGG - chr8_2:62672175-62672194 Msa1260630:three_prime_UTR 45.0%
GTTATTGAACTCGCCGGAAA+AGG + chr8_2:62672191-62672210 None:intergenic 45.0%
GAGCATGTTATTGAACTCGC+CGG + chr8_2:62672197-62672216 None:intergenic 45.0%
!!! TTTTTTGGAGAGGAAAGCGG+TGG + chr8_2:62672315-62672334 None:intergenic 45.0%
! TTCCTTGTATTTTCCCGGAG+GGG + chr8_2:62672445-62672464 None:intergenic 45.0%
! GATTCCTTGTATTTTCCCGG+AGG + chr8_2:62672447-62672466 None:intergenic 45.0%
GAAACTTGGCGAAACGTTGT+GGG + chr8_2:62672469-62672488 None:intergenic 45.0%
TTTGGGAATTTAGGAGGGGA+GGG - chr8_2:62672813-62672832 Msa1260630:intron 45.0%
GAGGGGAGGGTTTAAAAACT+AGG - chr8_2:62672826-62672845 Msa1260630:intron 45.0%
AGGAGGAAGCTGAAGGAATA+TGG - chr8_2:62672851-62672870 Msa1260630:intron 45.0%
! GAGCTAGTGCTGTTAGATAG+TGG - chr8_2:62672924-62672943 Msa1260630:intron 45.0%
! CTGTTAGATAGTGGTCATCG+TGG - chr8_2:62672933-62672952 Msa1260630:intron 45.0%
CGCTTCACAAATTGCTATGG+CGG + chr8_2:62672970-62672989 None:intergenic 45.0%
TGGCGCTTCACAAATTGCTA+TGG + chr8_2:62672973-62672992 None:intergenic 45.0%
TATAGTGCCGCAATGGTAGA+TGG + chr8_2:62672993-62673012 None:intergenic 45.0%
ATCTACCATTGCGGCACTAT+AGG - chr8_2:62672992-62673011 Msa1260630:intron 45.0%
!! TCTGGCAGTTTATGGTGGAT+TGG + chr8_2:62673050-62673069 None:intergenic 45.0%
AGCTTGAACTGATGTTGCAG+TGG + chr8_2:62673075-62673094 None:intergenic 45.0%
ACTGCAACATCAGTTCAAGC+TGG - chr8_2:62673074-62673093 Msa1260630:intron 45.0%
ATCCTGAAGGTGGAAAAGGA+GGG + chr8_2:62673163-62673182 None:intergenic 45.0%
CTACATCCTGAAGGTGGAAA+AGG + chr8_2:62673167-62673186 None:intergenic 45.0%
TCATTGCTACATCCTGAAGG+TGG + chr8_2:62673173-62673192 None:intergenic 45.0%
GGATCATTGCTACATCCTGA+AGG + chr8_2:62673176-62673195 None:intergenic 45.0%
TGAAATAGCCCAAGCTAAGC+AGG - chr8_2:62673416-62673435 Msa1260630:intron 45.0%
AATGACGTCCTGCTTAGCTT+GGG + chr8_2:62673427-62673446 None:intergenic 45.0%
AAATGACGTCCTGCTTAGCT+TGG + chr8_2:62673428-62673447 None:intergenic 45.0%
TCCTCTTCTGGTAACTAGCT+GGG - chr8_2:62673785-62673804 Msa1260630:intron 45.0%
GATCCCAAACACTAACTCTG+GGG + chr8_2:62674008-62674027 None:intergenic 45.0%
GGATCCCAAACACTAACTCT+GGG + chr8_2:62674009-62674028 None:intergenic 45.0%
AGGATCCCAAACACTAACTC+TGG + chr8_2:62674010-62674029 None:intergenic 45.0%
AAGCATCATGGCTAACACCA+AGG + chr8_2:62674041-62674060 None:intergenic 45.0%
AACAGTATGCTGGACATGAG+AGG - chr8_2:62674100-62674119 Msa1260630:CDS 45.0%
! GAGGAGCGTAGAATCAATAG+AGG - chr8_2:62674119-62674138 Msa1260630:CDS 45.0%
!! TGGCTAGTTTCGTACAAAGG+AGG - chr8_2:62674163-62674182 Msa1260630:intron 45.0%
TTTCTCTTTGCCTTCATGGG+TGG - chr8_2:62674306-62674325 Msa1260630:five_prime_UTR 45.0%
!! TGAAACTGGACCTGCTTTTC+AGG - chr8_2:62674350-62674369 Msa1260630:five_prime_UTR 45.0%
!! ATAAAAAAATAAAGAAATAT+AGG - chr8_2:62674460-62674479 Msa1260630:five_prime_UTR 5.0%
! GGAACGCGTTCGTTTCGTTT+TGG + chr8_2:62672170-62672189 None:intergenic 50.0%
AGGAAAGCGGTGGCGAAATT+GGG + chr8_2:62672305-62672324 None:intergenic 50.0%
CGAAACTTGGCGAAACGTTG+TGG + chr8_2:62672470-62672489 None:intergenic 50.0%
TGCGCTGTAGACACACCATA+TGG - chr8_2:62672502-62672521 Msa1260630:CDS 50.0%
GCTGTAGACACACCATATGG+AGG - chr8_2:62672505-62672524 Msa1260630:CDS 50.0%
GTTTGGGAATTTAGGAGGGG+AGG - chr8_2:62672812-62672831 Msa1260630:intron 50.0%
GATGTACTAGAGCCAAGCAC+AGG - chr8_2:62672896-62672915 Msa1260630:intron 50.0%
CTAGCTCAACAACCTGTGCT+TGG + chr8_2:62672911-62672930 None:intergenic 50.0%
! TGAAGCGCCATCTACCATTG+CGG - chr8_2:62672983-62673002 Msa1260630:intron 50.0%
TGCAACCTATAGTGCCGCAA+TGG + chr8_2:62673000-62673019 None:intergenic 50.0%
TGGTGTCTGGCAGTTTATGG+TGG + chr8_2:62673055-62673074 None:intergenic 50.0%
CAGTGGTGTCTGGCAGTTTA+TGG + chr8_2:62673058-62673077 None:intergenic 50.0%
ACTGATGTTGCAGTGGTGTC+TGG + chr8_2:62673068-62673087 None:intergenic 50.0%
TGAAGGTGGAAAAGGAGGGT+GGG + chr8_2:62673159-62673178 None:intergenic 50.0%
CATCCTGAAGGTGGAAAAGG+AGG + chr8_2:62673164-62673183 None:intergenic 50.0%
GCTAAGCAGGACGTCATTTC+AGG - chr8_2:62673429-62673448 Msa1260630:intron 50.0%
! AAGGATAGAGGGAAGGCATG+CGG - chr8_2:62673478-62673497 Msa1260630:intron 50.0%
CTCTTCGACAGAGTGACAGT+TGG + chr8_2:62673595-62673614 None:intergenic 50.0%
CAACTGTCACTCTGTCGAAG+AGG - chr8_2:62673593-62673612 Msa1260630:intron 50.0%
GTCCTCTTCTGGTAACTAGC+TGG - chr8_2:62673784-62673803 Msa1260630:intron 50.0%
ACCCAGCTAGTTACCAGAAG+AGG + chr8_2:62673789-62673808 None:intergenic 50.0%
TCTTCTGGTAACTAGCTGGG+TGG - chr8_2:62673788-62673807 Msa1260630:intron 50.0%
TGGTAACTAGCTGGGTGGTT+TGG - chr8_2:62673793-62673812 Msa1260630:intron 50.0%
CACATTCTTGACCCATGGTG+GGG + chr8_2:62674238-62674257 None:intergenic 50.0%
GCACATTCTTGACCCATGGT+GGG + chr8_2:62674239-62674258 None:intergenic 50.0%
AGCACATTCTTGACCCATGG+TGG + chr8_2:62674240-62674259 None:intergenic 50.0%
CACACGGCGCATTCTAGCAT+TGG - chr8_2:62672080-62672099 Msa1260630:three_prime_UTR 55.0%
! ACGGCGCATTCTAGCATTGG+AGG - chr8_2:62672083-62672102 Msa1260630:three_prime_UTR 55.0%
!! CGTTTTGGTCTGCAAGAGGG+AGG + chr8_2:62672155-62672174 None:intergenic 55.0%
GAGGAAAGCGGTGGCGAAAT+TGG + chr8_2:62672306-62672325 None:intergenic 55.0%
CTAGGGAAGGAGGAAGCTGA+AGG - chr8_2:62672844-62672863 Msa1260630:intron 55.0%
CTGAAGGTGGAAAAGGAGGG+TGG + chr8_2:62673160-62673179 None:intergenic 55.0%
! CACCCTCCTTTTCCACCTTC+AGG - chr8_2:62673158-62673177 Msa1260630:intron 55.0%
! AGGATAGAGGGAAGGCATGC+GGG - chr8_2:62673479-62673498 Msa1260630:intron 55.0%
! GGAGCACTCTCCCTGTTGTA+TGG + chr8_2:62673520-62673539 None:intergenic 55.0%
!! CTAACACCAAGGCGTCGGTA+AGG + chr8_2:62674030-62674049 None:intergenic 55.0%
CATGGCTAACACCAAGGCGT+CGG + chr8_2:62674035-62674054 None:intergenic 55.0%
AAGTTGAGGAGTCCCCACCA+TGG - chr8_2:62674223-62674242 Msa1260630:intron 55.0%
AGTTGAGGAGTCCCCACCAT+GGG - chr8_2:62674224-62674243 Msa1260630:intron 55.0%
TCTTTGCCTTCATGGGTGGC+TGG - chr8_2:62674310-62674329 Msa1260630:five_prime_UTR 55.0%
AATGCTCCAGCCACCCATGA+AGG + chr8_2:62674319-62674338 None:intergenic 55.0%
TTGGATTCTGCAGGCCCCTC+CGG - chr8_2:62672428-62672447 Msa1260630:CDS 60.0%
GGCCCCTCCGGGAAAATACA+AGG - chr8_2:62672440-62672459 Msa1260630:CDS 60.0%
GGAAAAGGAGGGTGGGAGCA+AGG + chr8_2:62673152-62673171 None:intergenic 60.0%
! GGATAGAGGGAAGGCATGCG+GGG - chr8_2:62673480-62673499 Msa1260630:intron 60.0%
! GATAGAGGGAAGGCATGCGG+GGG - chr8_2:62673481-62673500 Msa1260630:intron 60.0%
TGGGATCCTTACCGACGCCT+TGG - chr8_2:62674021-62674040 Msa1260630:intron 60.0%
TGGATTCTGCAGGCCCCTCC+GGG - chr8_2:62672429-62672448 Msa1260630:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 62672062 62674517 62672062 ID=Msa1260630;Name=Msa1260630
chr8_2 mRNA 62672062 62674517 62672062 ID=Msa1260630-mRNA-1;Parent=Msa1260630;Name=Msa1260630-mRNA-1;_AED=0.02;_eAED=0.02;_QI=216|1|1|1|1|1|3|140|177
chr8_2 exon 62672062 62672590 62672062 ID=Msa1260630-mRNA-1:exon:19933;Parent=Msa1260630-mRNA-1
chr8_2 exon 62674053 62674138 62674053 ID=Msa1260630-mRNA-1:exon:19932;Parent=Msa1260630-mRNA-1
chr8_2 exon 62674243 62674517 62674243 ID=Msa1260630-mRNA-1:exon:19931;Parent=Msa1260630-mRNA-1
chr8_2 five_prime_UTR 62674302 62674517 62674302 ID=Msa1260630-mRNA-1:five_prime_utr;Parent=Msa1260630-mRNA-1
chr8_2 CDS 62674243 62674301 62674243 ID=Msa1260630-mRNA-1:cds;Parent=Msa1260630-mRNA-1
chr8_2 CDS 62674053 62674138 62674053 ID=Msa1260630-mRNA-1:cds;Parent=Msa1260630-mRNA-1
chr8_2 CDS 62672202 62672590 62672202 ID=Msa1260630-mRNA-1:cds;Parent=Msa1260630-mRNA-1
chr8_2 three_prime_UTR 62672062 62672201 62672062 ID=Msa1260630-mRNA-1:three_prime_utr;Parent=Msa1260630-mRNA-1
Gene Sequence

>Msa1260630

ATGGCTTCTCTTTCACTTTCATCTCCCAATTTCGCCACCGCTTTCCTCTCCAAAAAACTGCCCCTCCGGGAAAATACAAGGAATCCCACAACGTTTCGCCAAGTTTCGTTTCGTACTAAGTGCGCTGTAGACACACCATATGGAGGTAATGTCCCAAAATTCCCCAGAGTTAGTGTTTGGGATCCTTACCGACGCCTTGGTGTTAGCCATGATGCTTCTGAAGAAGAAATTTGGGGGTCAAGAAATTTTCTGTTGCAACAGTATGCTGGACATGAGAGGAGCGTAGAATCAATAGAGGCTGCTTTTGAAAAAATATTGATGGCTAGTTTCGTACAAAGGAGGAAAACAAAAATTAATTTGAAAAGCAAGTTGAAGAAGAAAGTTGAGGAGTCCCCACCATGGGTCAAGAATGTGCTAAACATTGTTGAATTTCCACCAACTGAAATTATCCTCAGAAGATTGTTTCTCTTTGCCTTCATGGGTGGCTGGAGCATTATGAATTCTGCTGAAACTGGACCTGCTTTTCAGGTTTGA
Protein sequence

>Msa1260630

MASLSLSSPNFATAFLSKKLPLRENTRNPTTFRQVSFRTKCAVDTPYGGNVPKFPRVSVWDPYRRLGVSHDASEEEIWGSRNFLLQQYAGHERSVESIEAAFEKILMASFVQRRKTKINLKSKLKKKVEESPPWVKNVLNIVEFPPTEIILRRLFLFAFMGGWSIMNSAETGPAFQV*