AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1265690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265690 MtrunA17_Chr4g0071811 97.889 379 6 2 1 378 1 378 0.0 751
Msa1265690 MtrunA17_Chr2g0298711 39.175 291 137 9 36 308 43 311 1.93e-41 147
Msa1265690 MtrunA17_Chr5g0431351 46.154 117 58 3 262 378 99 210 8.52e-21 89.7
Msa1265690 MtrunA17_Chr7g0226451 34.591 159 66 5 255 378 190 345 1.12e-16 80.5
Msa1265690 MtrunA17_Chr1g0209861 43.519 108 49 4 283 378 311 418 1.17e-15 78.2
Msa1265690 MtrunA17_Chr8g0390971 42.157 102 47 1 289 378 293 394 1.86e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265690 AT5G57180.1 46.485 441 153 19 1 379 1 420 3.15e-77 244
Msa1265690 AT5G57180.2 46.364 440 153 19 1 378 1 419 2.52e-76 243
Msa1265690 AT4G25990.3 42.857 413 167 17 1 379 1 378 3.41e-67 217
Msa1265690 AT4G25990.2 42.857 413 167 17 1 379 1 378 1.14e-66 217
Msa1265690 AT4G25990.1 42.718 412 167 17 1 378 1 377 2.97e-66 215
Msa1265690 AT5G57180.3 41.688 391 149 18 1 329 1 374 2.03e-46 163
Msa1265690 AT5G57180.4 41.688 391 149 18 1 329 1 374 2.03e-46 163
Msa1265690 AT5G14370.1 44.860 107 38 4 288 378 229 330 1.17e-16 80.9
Msa1265690 AT1G07050.2 49.462 93 40 2 287 379 103 188 2.02e-16 77.4
Msa1265690 AT1G25440.1 36.170 141 73 2 252 378 260 397 2.26e-16 80.5
Msa1265690 AT1G07050.1 48.913 92 40 2 287 378 103 187 1.15e-15 75.5
Msa1265690 AT1G73870.1 44.444 108 46 3 285 378 274 381 4.07e-15 76.6
Msa1265690 AT1G68520.1 36.154 130 58 3 267 378 271 393 4.22e-14 73.6

Find 128 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACCGACACTGCATATTT+TGG 0.094471 8_2:-70225397 None:intergenic
TCCCGAATTTATATGAATTA+AGG 0.133453 8_2:-70224391 None:intergenic
TATGATATGGTTATGATAAT+TGG 0.154427 8_2:+70226678 Msa1265690:three_prime_UTR
TGACATGGTTTCACCGGTTT+TGG 0.239666 8_2:-70225078 None:intergenic
TCCCTTAATTCATATAAATT+CGG 0.248224 8_2:+70224389 Msa1265690:five_prime_UTR
ATTGATCATACCTTCATTCT+TGG 0.253571 8_2:-70226608 None:intergenic
GATTTATTATTGTTATTGTT+GGG 0.253877 8_2:-70224940 None:intergenic
AGCTTCGTTGTAGGTTTGAT+TGG 0.274583 8_2:-70224857 None:intergenic
TCTCATCCATGGATTGGTTT+TGG 0.279149 8_2:+70225261 Msa1265690:CDS
CCCGAATTTATATGAATTAA+GGG 0.284870 8_2:-70224390 None:intergenic
TTGGCGGGAATTTCGATTTC+CGG 0.294194 8_2:+70225280 Msa1265690:CDS
TGTCACAAATAGATTTGTTA+TGG 0.306145 8_2:+70226462 Msa1265690:CDS
CAATAATAAATCCACCAAAT+TGG 0.309576 8_2:+70224951 Msa1265690:CDS
AGGAAATCTAGTTTATGATA+TGG 0.321517 8_2:+70226665 Msa1265690:three_prime_UTR
TTGGGATTGGAGAAGAGGTT+TGG 0.329352 8_2:-70224904 None:intergenic
AACAAGCCTAACGCTTGTTT+TGG 0.329462 8_2:+70226883 Msa1265690:three_prime_UTR
GAGAGTAAAGAAAAGGAAAT+TGG 0.338392 8_2:-70224326 None:intergenic
GCTAAACCCTAAACCCAAAA+AGG 0.340692 8_2:-70224552 None:intergenic
TGCAGTCTGCACCAATGTTT+AGG 0.343838 8_2:+70226825 Msa1265690:three_prime_UTR
AATTGCATATGAGTGGAAAT+TGG 0.343867 8_2:-70224606 None:intergenic
GGATTTATTATTGTTATTGT+TGG 0.348745 8_2:-70224941 None:intergenic
GGGAATTTCGATTTCCGGTT+AGG 0.348859 8_2:+70225285 Msa1265690:CDS
GGGCTTGCCGTGAAGTAAAA+AGG 0.352021 8_2:-70225040 None:intergenic
GCCATGTTGATGAAGGAAAT+TGG 0.353307 8_2:+70225337 Msa1265690:CDS
CAGTCCTGTCTCCGATGCTT+CGG 0.355046 8_2:+70225599 Msa1265690:CDS
CCCTTAATTCATATAAATTC+GGG 0.360660 8_2:+70224390 Msa1265690:five_prime_UTR
ACCAGAAGTGAAATTCAAAA+AGG 0.363644 8_2:-70224636 None:intergenic
AATTGAATTACGACGGTGTT+CGG 0.364551 8_2:+70225538 Msa1265690:CDS
ACAAAGAACCTGAAATTGAT+TGG 0.366595 8_2:-70224457 None:intergenic
TTTCATACTCTTCAATTCAT+TGG 0.367471 8_2:+70224253 Msa1265690:five_prime_UTR
AGAAAATCACATATCAAGTT+AGG 0.372871 8_2:+70226564 Msa1265690:CDS
AATCATCACCACCATGTTCA+TGG 0.382343 8_2:+70224728 Msa1265690:CDS
TGGGATTGGAGAAGAGGTTT+GGG 0.389394 8_2:-70224903 None:intergenic
GTCACAAATAGATTTGTTAT+GGG 0.395534 8_2:+70226463 Msa1265690:CDS
AGGGAAAAGCGAGTTAAAAC+AGG 0.397845 8_2:-70224371 None:intergenic
TATTCTAAACTAAACCCCTT+TGG 0.406174 8_2:+70224490 Msa1265690:five_prime_UTR
GAGTGTTGCAGCTTCGTTGT+AGG 0.410073 8_2:-70224866 None:intergenic
TCTTCTTCTCATCCATGGAT+TGG 0.411520 8_2:+70225255 Msa1265690:CDS
CCCCTTTGTGGTTGTTGCGT+TGG 0.419219 8_2:+70226740 Msa1265690:three_prime_UTR
TTTGGCTCCGACTGGAAATC+GGG 0.427983 8_2:-70225060 None:intergenic
TGAGATTGGGATTGGAGAAG+AGG 0.428990 8_2:-70224909 None:intergenic
GGTTTGGGTATGCAGTGTTA+AGG 0.430567 8_2:-70224888 None:intergenic
TATAAGAGTTATAAGTAAGA+TGG 0.435947 8_2:-70224214 None:intergenic
GCTTCGTTGTAGGTTTGATT+GGG 0.439703 8_2:-70224856 None:intergenic
TTGTTATGGGAGAATGGAGG+AGG 0.440896 8_2:+70226476 Msa1265690:CDS
AGTGGCAGAGAGTAAAGAAA+AGG 0.440979 8_2:-70224333 None:intergenic
CACCTCTCTGTAATCCTAAC+CGG 0.443262 8_2:-70225299 None:intergenic
ATAGATTTGTTATGGGAGAA+TGG 0.448843 8_2:+70226470 Msa1265690:CDS
ACTTTCCTTTCTCCTCTCCT+CGG 0.448991 8_2:+70224297 Msa1265690:five_prime_UTR
GAATGCAGAATTGAAAGAAA+TGG 0.449165 8_2:+70225482 Msa1265690:CDS
CACCATTGAACTAATCTCTC+CGG 0.459584 8_2:-70225106 None:intergenic
TGGGAGAATGGAGGAGGAAG+TGG 0.459617 8_2:+70226482 Msa1265690:CDS
AGTCCTGTCTCCGATGCTTC+GGG 0.462905 8_2:+70225600 Msa1265690:CDS
AAGCGAGTTAAAACAGGGGC+AGG 0.464071 8_2:-70224365 None:intergenic
ACGTCTCTTCTTCTCATCCA+TGG 0.467379 8_2:+70225250 Msa1265690:CDS
ATTGCATATGAGTGGAAATT+GGG 0.468717 8_2:-70224605 None:intergenic
ACGACGGTGTTCGGAAAGCT+TGG 0.468820 8_2:+70225547 Msa1265690:CDS
ATAGATAGATCCTCAAAAGT+TGG 0.471366 8_2:-70226774 None:intergenic
ACCAACGCAACAACCACAAA+GGG 0.472287 8_2:-70226741 None:intergenic
ATTTATTATTGTTATTGTTG+GGG 0.476105 8_2:-70224939 None:intergenic
TTTCCAGTCGGAGCCAAAAC+CGG 0.481789 8_2:+70225065 Msa1265690:CDS
CTTTCCTTTCTCCTCTCCTC+GGG 0.482959 8_2:+70224298 Msa1265690:five_prime_UTR
ACTAGAAGAAATTAGAAAAG+AGG 0.485084 8_2:+70226645 Msa1265690:three_prime_UTR
TTTCCCGAAGCATCGGAGAC+AGG 0.498685 8_2:-70225603 None:intergenic
GGACTCTGACATGGTTTCAC+CGG 0.498907 8_2:-70225084 None:intergenic
AAGGAAAGTACAGTGAAGAA+AGG 0.501568 8_2:-70224283 None:intergenic
AACATCGTTTCCCGAAGCAT+CGG 0.505047 8_2:-70225610 None:intergenic
GAAAGTACAGTGAAGAAAGG+AGG 0.516615 8_2:-70224280 None:intergenic
TGGAAATTGGGTGAAATTGA+GGG 0.517764 8_2:-70224593 None:intergenic
GATTCAGAGATTGTTGAAGA+AGG 0.524561 8_2:-70224778 None:intergenic
TCCCGGAGAGATTAGTTCAA+TGG 0.528675 8_2:+70225104 Msa1265690:CDS
ACCAATTTCCTTCATCAACA+TGG 0.530695 8_2:-70225338 None:intergenic
CGGTTAGGATTACAGAGAGG+TGG 0.532921 8_2:+70225300 Msa1265690:CDS
GAATCGCCAAAGTTGAAAAG+TGG 0.536005 8_2:+70225504 Msa1265690:CDS
AAAGTACAGTGAAGAAAGGA+GGG 0.536683 8_2:-70224279 None:intergenic
GTGGAAATTGGGTGAAATTG+AGG 0.537129 8_2:-70224594 None:intergenic
GCTCTTCGCCATGTTGATGA+AGG 0.541149 8_2:+70225330 Msa1265690:CDS
ACCATTGAACTAATCTCTCC+GGG 0.545916 8_2:-70225105 None:intergenic
AGAAGGAATTGATAGCATCA+TGG 0.548899 8_2:+70225200 Msa1265690:CDS
GAAGGAATTGATAGCATCAT+GGG 0.550516 8_2:+70225201 Msa1265690:CDS
TTCCCGCCAAAACCAATCCA+TGG 0.558777 8_2:-70225267 None:intergenic
GTTGCCCGAGGAGAGGAGAA+AGG 0.559676 8_2:-70224302 None:intergenic
CTCGACGAGGAAATTGAAGA+AGG 0.561335 8_2:+70225183 Msa1265690:CDS
CGGTGGTAAGACGACGGCGC+CGG 0.566016 8_2:+70225413 Msa1265690:CDS
ATCGGAGACAGGACTGTCAT+CGG 0.567406 8_2:-70225592 None:intergenic
GACGTGTCCAATACTCTGAA+AGG 0.567728 8_2:-70225012 None:intergenic
ATATAGCCAAAACAAGCGTT+AGG 0.568478 8_2:-70226889 None:intergenic
TTCTTTGTAACGCAGCACGC+TGG 0.571082 8_2:-70226516 None:intergenic
AGATCGACGGCCAAGAATGA+AGG 0.572545 8_2:+70226598 Msa1265690:CDS
GTGAAACCATGTCAGAGTCC+CGG 0.575623 8_2:+70225087 Msa1265690:CDS
AATTAAGAATTGCATATGAG+TGG 0.578762 8_2:-70224613 None:intergenic
AATCACATATCAAGTTAGGA+AGG 0.579412 8_2:+70226568 Msa1265690:CDS
GGAACAGGAGGAAGAACCAT+CGG 0.583201 8_2:+70224685 Msa1265690:CDS
GAACAGGAGGAAGAACCATC+GGG 0.586253 8_2:+70224686 Msa1265690:CDS
ATCTCTCCGGGACTCTGACA+TGG 0.592091 8_2:-70225093 None:intergenic
GACCAACGCAACAACCACAA+AGG 0.593303 8_2:-70226742 None:intergenic
AATTGGTGTTGCCCGAGGAG+AGG 0.595304 8_2:-70224309 None:intergenic
AGGAGGAAGAACCATCGGGT+TGG 0.598540 8_2:+70224690 Msa1265690:CDS
AGGGGCAGGGCATTGTAAAG+TGG 0.599210 8_2:-70224351 None:intergenic
CATAAAGAAAACCTAAACAT+TGG 0.606506 8_2:-70226836 None:intergenic
TAAGACGACGGCGCCGGTGA+CGG 0.606872 8_2:+70225419 Msa1265690:CDS
CCCAAAATATGCAGTGTCGG+TGG 0.613838 8_2:+70225396 Msa1265690:CDS
ACAGGACTGTCATCGGCGAA+CGG 0.617792 8_2:-70225585 None:intergenic
CATCATGGGAGGTAGAATTG+AGG 0.620329 8_2:+70225215 Msa1265690:CDS
TCGCCCAAAATATGCAGTGT+CGG 0.623873 8_2:+70225393 Msa1265690:CDS
GATTTGTTATGGGAGAATGG+AGG 0.628311 8_2:+70226473 Msa1265690:CDS
AAATTGGGTGAAATTGAGGG+TGG 0.630325 8_2:-70224590 None:intergenic
GGAAGGTCAATGCAGATCGA+CGG 0.632746 8_2:+70226585 Msa1265690:CDS
AATTGGGTGAAATTGAGGGT+GGG 0.636386 8_2:-70224589 None:intergenic
GGGAAAAGCGAGTTAAAACA+GGG 0.641077 8_2:-70224370 None:intergenic
CGCGGAATCAATACTCGACG+AGG 0.646026 8_2:+70225170 Msa1265690:CDS
TATATCTAAATCCAACCCGA+TGG 0.646645 8_2:-70224701 None:intergenic
CGGCAAGCCCGATTTCCAGT+CGG 0.652179 8_2:+70225053 Msa1265690:CDS
CGGAAAGCTTGGTCTGACCG+AGG 0.657705 8_2:+70225558 Msa1265690:CDS
AAGGAAATTGGTGTTGCCCG+AGG 0.659053 8_2:-70224314 None:intergenic
TTTGTGGTGTAGATTGCAAG+CGG 0.661097 8_2:-70224811 None:intergenic
AGCGAGTTAAAACAGGGGCA+GGG 0.662824 8_2:-70224364 None:intergenic
GATGTTCGACTCCATGAACA+TGG 0.682341 8_2:-70224739 None:intergenic
TTGTTGAAATTGAATTACGA+CGG 0.684346 8_2:+70225531 Msa1265690:CDS
CAACGCAACAACCACAAAGG+GGG 0.685338 8_2:-70226739 None:intergenic
GGAATTGATAGCATCATGGG+AGG 0.697923 8_2:+70225204 Msa1265690:CDS
CAGTGTCGGTGGTAAGACGA+CGG 0.700394 8_2:+70225407 Msa1265690:CDS
ATGTTGATGAAGGAAATTGG+TGG 0.707203 8_2:+70225340 Msa1265690:CDS
TTCCGGTTAGGATTACAGAG+AGG 0.714429 8_2:+70225297 Msa1265690:CDS
GTACAGTGAAGAAAGGAGGG+TGG 0.717854 8_2:-70224276 None:intergenic
CCAACGCAACAACCACAAAG+GGG 0.718227 8_2:-70226740 None:intergenic
GGAAAAGCGAGTTAAAACAG+GGG 0.788071 8_2:-70224369 None:intergenic
GTTCGACTCCATGAACATGG+TGG 0.820791 8_2:-70224736 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATTATTGTTATTGTTG+GGG - chr8_2:70224942-70224961 None:intergenic 15.0%
!!! GATTTATTATTGTTATTGTT+GGG - chr8_2:70224943-70224962 None:intergenic 15.0%
!! AAGTAAAGTAAAAAATGAAT+GGG - chr8_2:70225976-70225995 None:intergenic 15.0%
!! AAAGTAAAGTAAAAAATGAA+TGG - chr8_2:70225977-70225996 None:intergenic 15.0%
!!! TTTTTTTGTGAAATAGAAAA+AGG - chr8_2:70226012-70226031 None:intergenic 15.0%
!! ATTCTCTTACAATATTTAAA+TGG + chr8_2:70226039-70226058 Msa1265690:intron 15.0%
!! TTCTCTTACAATATTTAAAT+GGG + chr8_2:70226040-70226059 Msa1265690:intron 15.0%
!! AAATTGTTTATTGATATTGT+GGG + chr8_2:70226426-70226445 Msa1265690:intron 15.0%
!! TATAAGAGTTATAAGTAAGA+TGG - chr8_2:70224217-70224236 None:intergenic 20.0%
!! TCCCTTAATTCATATAAATT+CGG + chr8_2:70224389-70224408 Msa1265690:five_prime_UTR 20.0%
!! TTTAAAAACCAATCAATTTC+AGG + chr8_2:70224449-70224468 Msa1265690:five_prime_UTR 20.0%
!!! GGATTTATTATTGTTATTGT+TGG - chr8_2:70224944-70224963 None:intergenic 20.0%
!! AAAAGAAAAAAAAAGAGAGA+GGG - chr8_2:70225847-70225866 None:intergenic 20.0%
!! AAAAAGAAAAAAAAAGAGAG+AGG - chr8_2:70225848-70225867 None:intergenic 20.0%
!!! TTTTTTTTTTAAGATTACCC+AGG + chr8_2:70225872-70225891 Msa1265690:intron 20.0%
!! AGTAAAGTAAAAAATGAATG+GGG - chr8_2:70225975-70225994 None:intergenic 20.0%
!! GAATATGTTACTCAATAAAA+AGG + chr8_2:70226067-70226086 Msa1265690:intron 20.0%
!!! AAGTAAAAAGATATTTGTTG+TGG + chr8_2:70226093-70226112 Msa1265690:intron 20.0%
!!! TTAAGATTTTGTCTTGAATT+AGG + chr8_2:70226228-70226247 Msa1265690:intron 20.0%
!!! AATTAGGTAAATTTTGTCTT+GGG + chr8_2:70226244-70226263 Msa1265690:intron 20.0%
!! AGTAGCATATGTTATAATTT+AGG + chr8_2:70226305-70226324 Msa1265690:intron 20.0%
!! TATTTAATAGATAGTGAATG+AGG + chr8_2:70226383-70226402 Msa1265690:intron 20.0%
!! ATTTAATAGATAGTGAATGA+GGG + chr8_2:70226384-70226403 Msa1265690:intron 20.0%
!! CAAATTGTTTATTGATATTG+TGG + chr8_2:70226425-70226444 Msa1265690:intron 20.0%
!! TATGATATGGTTATGATAAT+TGG + chr8_2:70226678-70226697 Msa1265690:three_prime_UTR 20.0%
! TTTCATACTCTTCAATTCAT+TGG + chr8_2:70224253-70224272 Msa1265690:five_prime_UTR 25.0%
! CCCGAATTTATATGAATTAA+GGG - chr8_2:70224393-70224412 None:intergenic 25.0%
! CCCTTAATTCATATAAATTC+GGG + chr8_2:70224390-70224409 Msa1265690:five_prime_UTR 25.0%
! TCCCGAATTTATATGAATTA+AGG - chr8_2:70224394-70224413 None:intergenic 25.0%
! AAAATGAAGAAAAAGCCAAA+GGG - chr8_2:70224508-70224527 None:intergenic 25.0%
! TAAAATGAAGAAAAAGCCAA+AGG - chr8_2:70224509-70224528 None:intergenic 25.0%
! AATTAAGAATTGCATATGAG+TGG - chr8_2:70224616-70224635 None:intergenic 25.0%
! CAATAATAAATCCACCAAAT+TGG + chr8_2:70224951-70224970 Msa1265690:CDS 25.0%
!!! TTCATAAGCTTTTTCCAATT+TGG - chr8_2:70224968-70224987 None:intergenic 25.0%
! TTGTTGAAATTGAATTACGA+CGG + chr8_2:70225531-70225550 Msa1265690:CDS 25.0%
!! AGCATTGAAATTGGTAATTA+AGG - chr8_2:70225701-70225720 None:intergenic 25.0%
!! ATTTTTAATCACGTGGAATA+TGG + chr8_2:70225899-70225918 Msa1265690:intron 25.0%
!! TTTTTAATCACGTGGAATAT+GGG + chr8_2:70225900-70225919 Msa1265690:intron 25.0%
!! GAATTAGGTAAATTTTGTCT+TGG + chr8_2:70226243-70226262 Msa1265690:intron 25.0%
!!! TGTCACAAATAGATTTGTTA+TGG + chr8_2:70226462-70226481 Msa1265690:CDS 25.0%
!!! GTCACAAATAGATTTGTTAT+GGG + chr8_2:70226463-70226482 Msa1265690:CDS 25.0%
! AGAAAATCACATATCAAGTT+AGG + chr8_2:70226564-70226583 Msa1265690:CDS 25.0%
! ACTAGAAGAAATTAGAAAAG+AGG + chr8_2:70226645-70226664 Msa1265690:three_prime_UTR 25.0%
!! AGGAAATCTAGTTTATGATA+TGG + chr8_2:70226665-70226684 Msa1265690:three_prime_UTR 25.0%
! CATAAAGAAAACCTAAACAT+TGG - chr8_2:70226839-70226858 None:intergenic 25.0%
GAGAGTAAAGAAAAGGAAAT+TGG - chr8_2:70224329-70224348 None:intergenic 30.0%
ACAAAGAACCTGAAATTGAT+TGG - chr8_2:70224460-70224479 None:intergenic 30.0%
TATTCTAAACTAAACCCCTT+TGG + chr8_2:70224490-70224509 Msa1265690:five_prime_UTR 30.0%
AAATGAAGAAAAAGCCAAAG+GGG - chr8_2:70224507-70224526 None:intergenic 30.0%
ATTGCATATGAGTGGAAATT+GGG - chr8_2:70224608-70224627 None:intergenic 30.0%
AATTGCATATGAGTGGAAAT+TGG - chr8_2:70224609-70224628 None:intergenic 30.0%
! TCCTTTTTGAATTTCACTTC+TGG + chr8_2:70224635-70224654 Msa1265690:five_prime_UTR 30.0%
ACCAGAAGTGAAATTCAAAA+AGG - chr8_2:70224639-70224658 None:intergenic 30.0%
!! AAAATGTCTTCGTGTTTTAG+TGG + chr8_2:70224664-70224683 Msa1265690:exon 30.0%
!! ATAAGCTTTTTCCAATTTGG+TGG - chr8_2:70224965-70224984 None:intergenic 30.0%
! TCTTTTACCTTTCAGAGTAT+TGG + chr8_2:70225005-70225024 Msa1265690:CDS 30.0%
! TCATCATCCTTTTTACTTCA+CGG + chr8_2:70225033-70225052 Msa1265690:CDS 30.0%
GAATGCAGAATTGAAAGAAA+TGG + chr8_2:70225482-70225501 Msa1265690:CDS 30.0%
!! AACAATCCACTTTTCAACTT+TGG - chr8_2:70225513-70225532 None:intergenic 30.0%
!!! ACAGGTGTTGAATTTTGATT+GGG - chr8_2:70225655-70225674 None:intergenic 30.0%
!!! AACAGGTGTTGAATTTTGAT+TGG - chr8_2:70225656-70225675 None:intergenic 30.0%
ACAAGTACGAAAACAAAAAC+AGG - chr8_2:70225673-70225692 None:intergenic 30.0%
!! GATTAAGGTACTGAAAATGT+CGG - chr8_2:70225731-70225750 None:intergenic 30.0%
AATTGACATATGAGGGATTA+AGG - chr8_2:70225746-70225765 None:intergenic 30.0%
GTTGTTCAATTGACATATGA+GGG - chr8_2:70225753-70225772 None:intergenic 30.0%
TGTTGTTCAATTGACATATG+AGG - chr8_2:70225754-70225773 None:intergenic 30.0%
CTTTGTGTAGTTTCAATAAC+CGG + chr8_2:70226191-70226210 Msa1265690:intron 30.0%
TTTGTCTTGGGTCAAAATTA+TGG + chr8_2:70226256-70226275 Msa1265690:intron 30.0%
TTGTCTTGGGTCAAAATTAT+GGG + chr8_2:70226257-70226276 Msa1265690:intron 30.0%
!! ATAGATTTGTTATGGGAGAA+TGG + chr8_2:70226470-70226489 Msa1265690:CDS 30.0%
! ATGTGATTTTCTTAGAGATG+AGG - chr8_2:70226556-70226575 None:intergenic 30.0%
AATCACATATCAAGTTAGGA+AGG + chr8_2:70226568-70226587 Msa1265690:CDS 30.0%
ATTGATCATACCTTCATTCT+TGG - chr8_2:70226611-70226630 None:intergenic 30.0%
!!! GCATCGTTCTTTTTTGTTTA+TGG + chr8_2:70226708-70226727 Msa1265690:three_prime_UTR 30.0%
!!! TCTTTTTTGTTTATGGAGAG+TGG + chr8_2:70226715-70226734 Msa1265690:three_prime_UTR 30.0%
ATAGATAGATCCTCAAAAGT+TGG - chr8_2:70226777-70226796 None:intergenic 30.0%
AAAGTACAGTGAAGAAAGGA+GGG - chr8_2:70224282-70224301 None:intergenic 35.0%
! AAGGAAAGTACAGTGAAGAA+AGG - chr8_2:70224286-70224305 None:intergenic 35.0%
! TTTTGTTCCCTCTACCTTTT+TGG + chr8_2:70224538-70224557 Msa1265690:five_prime_UTR 35.0%
! TTTGTTCCCTCTACCTTTTT+GGG + chr8_2:70224539-70224558 Msa1265690:five_prime_UTR 35.0%
TGGAAATTGGGTGAAATTGA+GGG - chr8_2:70224596-70224615 None:intergenic 35.0%
TATATCTAAATCCAACCCGA+TGG - chr8_2:70224704-70224723 None:intergenic 35.0%
GATTCAGAGATTGTTGAAGA+AGG - chr8_2:70224781-70224800 None:intergenic 35.0%
!! TTTGATTGGGTCGTTTTCTT+GGG - chr8_2:70224846-70224865 None:intergenic 35.0%
!!! GTTTGTTTTGAGATTGGGAT+TGG - chr8_2:70224920-70224939 None:intergenic 35.0%
!!! TTGGGGTTTGTTTTGAGATT+GGG - chr8_2:70224925-70224944 None:intergenic 35.0%
!! TGATGATGATGATTTTGACG+CGG + chr8_2:70225152-70225171 Msa1265690:CDS 35.0%
! AGAAGGAATTGATAGCATCA+TGG + chr8_2:70225200-70225219 Msa1265690:CDS 35.0%
! GAAGGAATTGATAGCATCAT+GGG + chr8_2:70225201-70225220 Msa1265690:CDS 35.0%
ACCAATTTCCTTCATCAACA+TGG - chr8_2:70225341-70225360 None:intergenic 35.0%
! ATGTTGATGAAGGAAATTGG+TGG + chr8_2:70225340-70225359 Msa1265690:CDS 35.0%
! AGGAAATTGGTGGAATTTTC+CGG + chr8_2:70225350-70225369 Msa1265690:CDS 35.0%
! ATTTTAAGCATATCAACCGC+CGG - chr8_2:70225372-70225391 None:intergenic 35.0%
GGAGAAGAAGAAAAAGAAGA+AGG + chr8_2:70225440-70225459 Msa1265690:CDS 35.0%
GAAAAAGAAGAAGGTTGTTG+CGG + chr8_2:70225449-70225468 Msa1265690:CDS 35.0%
AATTGAATTACGACGGTGTT+CGG + chr8_2:70225538-70225557 Msa1265690:CDS 35.0%
!! GGAAAACAGAGCATTGAAAT+TGG - chr8_2:70225710-70225729 None:intergenic 35.0%
CTTAACATGAACATACAGTC+CGG - chr8_2:70226213-70226232 None:intergenic 35.0%
TTATTGATATTGTGGGAGAC+AGG + chr8_2:70226433-70226452 Msa1265690:intron 35.0%
!! CACTTTTTGTCCAACTTTTG+AGG + chr8_2:70226764-70226783 Msa1265690:three_prime_UTR 35.0%
ATATAGCCAAAACAAGCGTT+AGG - chr8_2:70226892-70226911 None:intergenic 35.0%
! GAAAGTACAGTGAAGAAAGG+AGG - chr8_2:70224283-70224302 None:intergenic 40.0%
AGTGGCAGAGAGTAAAGAAA+AGG - chr8_2:70224336-70224355 None:intergenic 40.0%
GGAAAAGCGAGTTAAAACAG+GGG - chr8_2:70224372-70224391 None:intergenic 40.0%
GGGAAAAGCGAGTTAAAACA+GGG - chr8_2:70224373-70224392 None:intergenic 40.0%
AGGGAAAAGCGAGTTAAAAC+AGG - chr8_2:70224374-70224393 None:intergenic 40.0%
CCTAAACCCAAAAAGGTAGA+GGG - chr8_2:70224548-70224567 None:intergenic 40.0%
!!! CCTCTACCTTTTTGGGTTTA+GGG + chr8_2:70224546-70224565 Msa1265690:five_prime_UTR 40.0%
GCTAAACCCTAAACCCAAAA+AGG - chr8_2:70224555-70224574 None:intergenic 40.0%
AATTGGGTGAAATTGAGGGT+GGG - chr8_2:70224592-70224611 None:intergenic 40.0%
AAATTGGGTGAAATTGAGGG+TGG - chr8_2:70224593-70224612 None:intergenic 40.0%
GTGGAAATTGGGTGAAATTG+AGG - chr8_2:70224597-70224616 None:intergenic 40.0%
!!! TCTTCGTGTTTTAGTGGAAC+AGG + chr8_2:70224670-70224689 Msa1265690:CDS 40.0%
AATCATCACCACCATGTTCA+TGG + chr8_2:70224728-70224747 Msa1265690:CDS 40.0%
TTTGTGGTGTAGATTGCAAG+CGG - chr8_2:70224814-70224833 None:intergenic 40.0%
!! TTGATTGGGTCGTTTTCTTG+GGG - chr8_2:70224845-70224864 None:intergenic 40.0%
!! GTTTGATTGGGTCGTTTTCT+TGG - chr8_2:70224847-70224866 None:intergenic 40.0%
GCTTCGTTGTAGGTTTGATT+GGG - chr8_2:70224859-70224878 None:intergenic 40.0%
AGCTTCGTTGTAGGTTTGAT+TGG - chr8_2:70224860-70224879 None:intergenic 40.0%
!!! GTTGGGGTTTGTTTTGAGAT+TGG - chr8_2:70224926-70224945 None:intergenic 40.0%
ACCATTGAACTAATCTCTCC+GGG - chr8_2:70225108-70225127 None:intergenic 40.0%
CACCATTGAACTAATCTCTC+CGG - chr8_2:70225109-70225128 None:intergenic 40.0%
TCTTCTTCTCATCCATGGAT+TGG + chr8_2:70225255-70225274 Msa1265690:CDS 40.0%
!!! TCTCATCCATGGATTGGTTT+TGG + chr8_2:70225261-70225280 Msa1265690:CDS 40.0%
! GCCATGTTGATGAAGGAAAT+TGG + chr8_2:70225337-70225356 Msa1265690:CDS 40.0%
! AAATTGGTGGAATTTTCCGG+CGG + chr8_2:70225353-70225372 Msa1265690:CDS 40.0%
! TTTTTCTTCTTCTCCGTCAC+CGG - chr8_2:70225435-70225454 None:intergenic 40.0%
GAATCGCCAAAGTTGAAAAG+TGG + chr8_2:70225504-70225523 Msa1265690:CDS 40.0%
CGTGATTAAAAATCTCGCCT+GGG - chr8_2:70225892-70225911 None:intergenic 40.0%
ACGTGATTAAAAATCTCGCC+TGG - chr8_2:70225893-70225912 None:intergenic 40.0%
! AGGCGAGATTTTTAATCACG+TGG + chr8_2:70225892-70225911 Msa1265690:intron 40.0%
!! GATTTGTTATGGGAGAATGG+AGG + chr8_2:70226473-70226492 Msa1265690:CDS 40.0%
! AACAAGCCTAACGCTTGTTT+TGG + chr8_2:70226883-70226902 Msa1265690:three_prime_UTR 40.0%
ACTTTCCTTTCTCCTCTCCT+CGG + chr8_2:70224297-70224316 Msa1265690:five_prime_UTR 45.0%
!!! CCCTCTACCTTTTTGGGTTT+AGG + chr8_2:70224545-70224564 Msa1265690:five_prime_UTR 45.0%
CCCTAAACCCAAAAAGGTAG+AGG - chr8_2:70224549-70224568 None:intergenic 45.0%
!!! TCGTGTTTTAGTGGAACAGG+AGG + chr8_2:70224673-70224692 Msa1265690:CDS 45.0%
GATGTTCGACTCCATGAACA+TGG - chr8_2:70224742-70224761 None:intergenic 45.0%
GGTTTGGGTATGCAGTGTTA+AGG - chr8_2:70224891-70224910 None:intergenic 45.0%
TGGGATTGGAGAAGAGGTTT+GGG - chr8_2:70224906-70224925 None:intergenic 45.0%
TTGGGATTGGAGAAGAGGTT+TGG - chr8_2:70224907-70224926 None:intergenic 45.0%
TGAGATTGGGATTGGAGAAG+AGG - chr8_2:70224912-70224931 None:intergenic 45.0%
GACGTGTCCAATACTCTGAA+AGG - chr8_2:70225015-70225034 None:intergenic 45.0%
!!! TTTTGGCTCCGACTGGAAAT+CGG - chr8_2:70225064-70225083 None:intergenic 45.0%
!!! TGACATGGTTTCACCGGTTT+TGG - chr8_2:70225081-70225100 None:intergenic 45.0%
! TCCCGGAGAGATTAGTTCAA+TGG + chr8_2:70225104-70225123 Msa1265690:CDS 45.0%
CTCGACGAGGAAATTGAAGA+AGG + chr8_2:70225183-70225202 Msa1265690:CDS 45.0%
! GGAATTGATAGCATCATGGG+AGG + chr8_2:70225204-70225223 Msa1265690:CDS 45.0%
CATCATGGGAGGTAGAATTG+AGG + chr8_2:70225215-70225234 Msa1265690:CDS 45.0%
ACGTCTCTTCTTCTCATCCA+TGG + chr8_2:70225250-70225269 Msa1265690:CDS 45.0%
!!! CATCCATGGATTGGTTTTGG+CGG + chr8_2:70225264-70225283 Msa1265690:CDS 45.0%
!!! ATCCATGGATTGGTTTTGGC+GGG + chr8_2:70225265-70225284 Msa1265690:CDS 45.0%
TTGGCGGGAATTTCGATTTC+CGG + chr8_2:70225280-70225299 Msa1265690:CDS 45.0%
GGGAATTTCGATTTCCGGTT+AGG + chr8_2:70225285-70225304 Msa1265690:CDS 45.0%
TTCCGGTTAGGATTACAGAG+AGG + chr8_2:70225297-70225316 Msa1265690:CDS 45.0%
CACCTCTCTGTAATCCTAAC+CGG - chr8_2:70225302-70225321 None:intergenic 45.0%
TCGCCCAAAATATGCAGTGT+CGG + chr8_2:70225393-70225412 Msa1265690:CDS 45.0%
! CCACCGACACTGCATATTTT+GGG - chr8_2:70225399-70225418 None:intergenic 45.0%
! ACCACCGACACTGCATATTT+TGG - chr8_2:70225400-70225419 None:intergenic 45.0%
AACATCGTTTCCCGAAGCAT+CGG - chr8_2:70225613-70225632 None:intergenic 45.0%
TTGTTATGGGAGAATGGAGG+AGG + chr8_2:70226476-70226495 Msa1265690:CDS 45.0%
ACCAACGCAACAACCACAAA+GGG - chr8_2:70226744-70226763 None:intergenic 45.0%
TGCAGTCTGCACCAATGTTT+AGG + chr8_2:70226825-70226844 Msa1265690:three_prime_UTR 45.0%
GTACAGTGAAGAAAGGAGGG+TGG - chr8_2:70224279-70224298 None:intergenic 50.0%
CTTTCCTTTCTCCTCTCCTC+GGG + chr8_2:70224298-70224317 Msa1265690:five_prime_UTR 50.0%
!! AAGGAAATTGGTGTTGCCCG+AGG - chr8_2:70224317-70224336 None:intergenic 50.0%
AGCGAGTTAAAACAGGGGCA+GGG - chr8_2:70224367-70224386 None:intergenic 50.0%
AAGCGAGTTAAAACAGGGGC+AGG - chr8_2:70224368-70224387 None:intergenic 50.0%
GGAACAGGAGGAAGAACCAT+CGG + chr8_2:70224685-70224704 Msa1265690:CDS 50.0%
GAACAGGAGGAAGAACCATC+GGG + chr8_2:70224686-70224705 Msa1265690:CDS 50.0%
GTTCGACTCCATGAACATGG+TGG - chr8_2:70224739-70224758 None:intergenic 50.0%
!!! TCTTGGGGTTCTGGGTTTTG+TGG - chr8_2:70224830-70224849 None:intergenic 50.0%
!! GGTCGTTTTCTTGGGGTTCT+GGG - chr8_2:70224838-70224857 None:intergenic 50.0%
! GAGTGTTGCAGCTTCGTTGT+AGG - chr8_2:70224869-70224888 None:intergenic 50.0%
GGGCTTGCCGTGAAGTAAAA+AGG - chr8_2:70225043-70225062 None:intergenic 50.0%
! TTTGGCTCCGACTGGAAATC+GGG - chr8_2:70225063-70225082 None:intergenic 50.0%
TTTCCAGTCGGAGCCAAAAC+CGG + chr8_2:70225065-70225084 Msa1265690:CDS 50.0%
GGACTCTGACATGGTTTCAC+CGG - chr8_2:70225087-70225106 None:intergenic 50.0%
GTGAAACCATGTCAGAGTCC+CGG + chr8_2:70225087-70225106 Msa1265690:CDS 50.0%
TTCCCGCCAAAACCAATCCA+TGG - chr8_2:70225270-70225289 None:intergenic 50.0%
CGGTTAGGATTACAGAGAGG+TGG + chr8_2:70225300-70225319 Msa1265690:CDS 50.0%
! GCTCTTCGCCATGTTGATGA+AGG + chr8_2:70225330-70225349 Msa1265690:CDS 50.0%
! CCCAAAATATGCAGTGTCGG+TGG + chr8_2:70225396-70225415 Msa1265690:CDS 50.0%
ATCGGAGACAGGACTGTCAT+CGG - chr8_2:70225595-70225614 None:intergenic 50.0%
GATATTGTGGGAGACAGGCA+CGG + chr8_2:70226438-70226457 Msa1265690:intron 50.0%
TTCTTTGTAACGCAGCACGC+TGG - chr8_2:70226519-70226538 None:intergenic 50.0%
GGAAGGTCAATGCAGATCGA+CGG + chr8_2:70226585-70226604 Msa1265690:CDS 50.0%
AGATCGACGGCCAAGAATGA+AGG + chr8_2:70226598-70226617 Msa1265690:CDS 50.0%
CAACGCAACAACCACAAAGG+GGG - chr8_2:70226742-70226761 None:intergenic 50.0%
CCAACGCAACAACCACAAAG+GGG - chr8_2:70226743-70226762 None:intergenic 50.0%
GACCAACGCAACAACCACAA+AGG - chr8_2:70226745-70226764 None:intergenic 50.0%
!! AATTGGTGTTGCCCGAGGAG+AGG - chr8_2:70224312-70224331 None:intergenic 55.0%
!! AGGGGCAGGGCATTGTAAAG+TGG - chr8_2:70224354-70224373 None:intergenic 55.0%
AGGAGGAAGAACCATCGGGT+TGG + chr8_2:70224690-70224709 Msa1265690:CDS 55.0%
!! GGGTCGTTTTCTTGGGGTTC+TGG - chr8_2:70224839-70224858 None:intergenic 55.0%
ATCTCTCCGGGACTCTGACA+TGG - chr8_2:70225096-70225115 None:intergenic 55.0%
CGCGGAATCAATACTCGACG+AGG + chr8_2:70225170-70225189 Msa1265690:CDS 55.0%
!! CAGTGTCGGTGGTAAGACGA+CGG + chr8_2:70225407-70225426 Msa1265690:CDS 55.0%
ACGACGGTGTTCGGAAAGCT+TGG + chr8_2:70225547-70225566 Msa1265690:CDS 55.0%
ACAGGACTGTCATCGGCGAA+CGG - chr8_2:70225588-70225607 None:intergenic 55.0%
CAGTCCTGTCTCCGATGCTT+CGG + chr8_2:70225599-70225618 Msa1265690:CDS 55.0%
AGTCCTGTCTCCGATGCTTC+GGG + chr8_2:70225600-70225619 Msa1265690:CDS 55.0%
!! TTTCCCGAAGCATCGGAGAC+AGG - chr8_2:70225606-70225625 None:intergenic 55.0%
TGGGAGAATGGAGGAGGAAG+TGG + chr8_2:70226482-70226501 Msa1265690:CDS 55.0%
CCCCTTTGTGGTTGTTGCGT+TGG + chr8_2:70226740-70226759 Msa1265690:three_prime_UTR 55.0%
GTTGCCCGAGGAGAGGAGAA+AGG - chr8_2:70224305-70224324 None:intergenic 60.0%
CGGCAAGCCCGATTTCCAGT+CGG + chr8_2:70225053-70225072 Msa1265690:CDS 60.0%
!!! TCACCGGTTTTGGCTCCGAC+TGG - chr8_2:70225071-70225090 None:intergenic 60.0%
CGGAAAGCTTGGTCTGACCG+AGG + chr8_2:70225558-70225577 Msa1265690:CDS 60.0%
TGGAGAGTGGACCCCCTTTG+TGG + chr8_2:70226728-70226747 Msa1265690:three_prime_UTR 60.0%
!! TAAGACGACGGCGCCGGTGA+CGG + chr8_2:70225419-70225438 Msa1265690:CDS 65.0%
CATCGGCGAACGGCGTTCCT+CGG - chr8_2:70225578-70225597 None:intergenic 65.0%
CGGTGGTAAGACGACGGCGC+CGG + chr8_2:70225413-70225432 Msa1265690:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 70224193 70226952 70224193 ID=Msa1265690;Name=Msa1265690
chr8_2 mRNA 70224193 70226952 70224193 ID=Msa1265690-mRNA-1;Parent=Msa1265690;Name=Msa1265690-mRNA-1;_AED=0.08;_eAED=0.08;_QI=474|1|1|1|1|1|2|327|379
chr8_2 exon 70224193 70225635 70224193 ID=Msa1265690-mRNA-1:exon:23279;Parent=Msa1265690-mRNA-1
chr8_2 exon 70226455 70226952 70226455 ID=Msa1265690-mRNA-1:exon:23280;Parent=Msa1265690-mRNA-1
chr8_2 five_prime_UTR 70224193 70224666 70224193 ID=Msa1265690-mRNA-1:five_prime_utr;Parent=Msa1265690-mRNA-1
chr8_2 CDS 70224667 70225635 70224667 ID=Msa1265690-mRNA-1:cds;Parent=Msa1265690-mRNA-1
chr8_2 CDS 70226455 70226625 70226455 ID=Msa1265690-mRNA-1:cds;Parent=Msa1265690-mRNA-1
chr8_2 three_prime_UTR 70226626 70226952 70226626 ID=Msa1265690-mRNA-1:three_prime_utr;Parent=Msa1265690-mRNA-1
Gene Sequence

>Msa1265690

ATGTCTTCGTGTTTTAGTGGAACAGGAGGAAGAACCATCGGGTTGGATTTAGATATAGTTAAATCATCACCACCATGTTCATGGAGTCGAACATCACAAACATCTTCTTCACCTTCTTCAACAATCTCTGAATCAAGCAATTCACCGCTTGCAATCTACACCACAAAACCCAGAACCCCAAGAAAACGACCCAATCAAACCTACAACGAAGCTGCAACACTCCTTAACACTGCATACCCAAACCTCTTCTCCAATCCCAATCTCAAAACAAACCCCAACAATAACAATAATAAATCCACCAAATTGGAAAAAGCTTATGAATTCGACTCTTCTGAACTTCTTTTACCTTTCAGAGTATTGGACACGTCATCATCCTTTTTACTTCACGGCAAGCCCGATTTCCAGTCGGAGCCAAAACCGGTGAAACCATGTCAGAGTCCCGGAGAGATTAGTTCAATGGTGAATTGTCTTGAATTGAACGATGATGATGATGATGATTTTGACGCGGAATCAATACTCGACGAGGAAATTGAAGAAGGAATTGATAGCATCATGGGAGGTAGAATTGAGGAGAATGAATCTAACGTCTCTTCTTCTCATCCATGGATTGGTTTTGGCGGGAATTTCGATTTCCGGTTAGGATTACAGAGAGGTGGAGTGAGAGCTCTTCGCCATGTTGATGAAGGAAATTGGTGGAATTTTCCGGCGGTTGATATGCTTAAAATTTCGCCCAAAATATGCAGTGTCGGTGGTAAGACGACGGCGCCGGTGACGGAGAAGAAGAAAAAGAAGAAGGTTGTTGCGGTAGAGAGATTGAATGCAGAATTGAAAGAAATGGAATCGCCAAAGTTGAAAAGTGGATTGTTGTTGAAATTGAATTACGACGGTGTTCGGAAAGCTTGGTCTGACCGAGGAACGCCGTTCGCCGATGACAGTCCTGTCTCCGATGCTTCGGGAAACGATGTTAATGCACGGTTGTCACAAATAGATTTGTTATGGGAGAATGGAGGAGGAAGTGGTGTAAGAGAAGCCAGCGTGCTGCGTTACAAAGAAAAGCGTCGTACACGCCTCATCTCTAAGAAAATCACATATCAAGTTAGGAAGGTCAATGCAGATCGACGGCCAAGAATGAAGGTATGA
Protein sequence

>Msa1265690

MSSCFSGTGGRTIGLDLDIVKSSPPCSWSRTSQTSSSPSSTISESSNSPLAIYTTKPRTPRKRPNQTYNEAATLLNTAYPNLFSNPNLKTNPNNNNNKSTKLEKAYEFDSSELLLPFRVLDTSSSFLLHGKPDFQSEPKPVKPCQSPGEISSMVNCLELNDDDDDDFDAESILDEEIEEGIDSIMGGRIEENESNVSSSHPWIGFGGNFDFRLGLQRGGVRALRHVDEGNWWNFPAVDMLKISPKICSVGGKTTAPVTEKKKKKKVVAVERLNAELKEMESPKLKSGLLLKLNYDGVRKAWSDRGTPFADDSPVSDASGNDVNARLSQIDLLWENGGGSGVREASVLRYKEKRRTRLISKKITYQVRKVNADRRPRMKV*