AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1276810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1276810 MtrunA17_Chr8g0344481 94.521 146 8 0 1 146 1 146 7.70e-99 284
Msa1276810 MtrunA17_Chr2g0301131 33.571 140 75 4 11 138 12 145 7.35e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1276810 AT1G72450.2 33.758 157 82 5 15 154 3 154 2.29e-12 62.4
Msa1276810 AT1G72450.1 33.758 157 82 5 15 154 3 154 2.09e-11 60.5

Find 62 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGCTTTCTTTGGGAAAA+TGG 0.109042 8_3:+11705684 Msa1276810:CDS
TTGAAGGAAAAGAGAATTTC+TGG 0.175716 8_3:+11705652 Msa1276810:CDS
AAGGCCACAACAATCTTCTT+AGG 0.212839 8_3:-11707873 None:intergenic
GATTCTACACCTGCTTTCTT+TGG 0.212849 8_3:+11705676 Msa1276810:CDS
TTTAGTTCATCTTGAACTTT+AGG 0.228550 8_3:+11707807 Msa1276810:three_prime_UTR
TTAAGAAGTGAAGCTCTTCT+TGG 0.236604 8_3:-11707540 None:intergenic
CTAAGCTTGTTCGTTGAATC+TGG 0.272218 8_3:-11705413 None:intergenic
GTGTTTGTTTGAGGAATTTC+GGG 0.278263 8_3:-11707337 None:intergenic
AACTAATTTACCTGAACATT+TGG 0.278582 8_3:-11707392 None:intergenic
TCCTTGTTCGGTTGTTTCTA+AGG 0.284638 8_3:-11707362 None:intergenic
GAACTTCTCCGGTGCCTTAC+CGG 0.289709 8_3:-11705593 None:intergenic
AGTGTTTGTTTGAGGAATTT+CGG 0.311669 8_3:-11707338 None:intergenic
AGAGGACCTTATCAAATTAA+TGG 0.313978 8_3:+11707705 Msa1276810:three_prime_UTR
TGATCGGAAGAATTATCTTT+AGG 0.316360 8_3:-11707762 None:intergenic
ACTTTAGGTTATACATGTTT+TGG 0.324420 8_3:+11707822 Msa1276810:three_prime_UTR
CAATTGACGATATTCTATTC+CGG 0.343572 8_3:+11707176 Msa1276810:CDS
TATTATGTTATCAATCAATT+AGG 0.344670 8_3:+11707938 Msa1276810:three_prime_UTR
TTTGGCTTGGTTTCCTTGTT+CGG 0.349316 8_3:-11707374 None:intergenic
ATTCTACACCTGCTTTCTTT+GGG 0.379166 8_3:+11705677 Msa1276810:CDS
AATCTTCTTAGGGCATAGTT+AGG 0.380172 8_3:-11707862 None:intergenic
GGAAACCAAGCCAAATGTTC+AGG 0.389691 8_3:+11707382 Msa1276810:three_prime_UTR
TCAATTAGGTCCAATGCTTT+CGG 0.391973 8_3:+11707952 Msa1276810:three_prime_UTR
TTGTGACCATTAATTTGATA+AGG 0.394801 8_3:-11707711 None:intergenic
CACTAGTGACCGGTGCACTT+GGG 0.400373 8_3:-11707300 None:intergenic
CTTGTTCGTTGAATCTGGTT+TGG 0.405449 8_3:-11705408 None:intergenic
AATTGACGATATTCTATTCC+GGG 0.411302 8_3:+11707177 Msa1276810:CDS
TCACTAGTGACCGGTGCACT+TGG 0.422956 8_3:-11707301 None:intergenic
TAAATCTATATGAAGATTGA+AGG 0.439481 8_3:-11707892 None:intergenic
AACTAAATCACTATAAGTTA+AGG 0.443726 8_3:-11707791 None:intergenic
GTGCCTTACCGGTGAGTCTC+CGG 0.457733 8_3:-11705582 None:intergenic
GCATTAAGAAGTCTAATTCA+AGG 0.463195 8_3:+11706111 Msa1276810:CDS
TGTGAAAATTTGAACTTCTC+CGG 0.466884 8_3:-11705604 None:intergenic
TCTAAGGCAGTGTTTGTTTG+AGG 0.469946 8_3:-11707346 None:intergenic
TTTGGGAAAATGGAACCTAA+AGG 0.472484 8_3:+11705694 Msa1276810:CDS
ACTAGTGACCGGTGCACTTG+GGG 0.487227 8_3:-11707299 None:intergenic
TACGAATGCAACTGAGATCA+TGG 0.488655 8_3:+11707229 Msa1276810:CDS
CTAATACTGTGTCGGATGGC+CGG 0.499466 8_3:+11705563 Msa1276810:CDS
AGGCCACAACAATCTTCTTA+GGG 0.511789 8_3:-11707872 None:intergenic
CCAACATGCAAAACTCAGAT+GGG 0.513652 8_3:+11706030 Msa1276810:CDS
ATTTACCTGAACATTTGGCT+TGG 0.519424 8_3:-11707387 None:intergenic
GAATTAGACTTCTTAATGCA+AGG 0.530933 8_3:-11706107 None:intergenic
TGAATCTCTTCCTCAGCTTG+TGG 0.531942 8_3:+11706079 Msa1276810:CDS
GCCAACATGCAAAACTCAGA+TGG 0.537855 8_3:+11706029 Msa1276810:CDS
TCGGATGGCCGGAGACTCAC+CGG 0.544946 8_3:+11705574 Msa1276810:CDS
TGCAACTGAGATCATGGAGT+TGG 0.557096 8_3:+11707235 Msa1276810:CDS
TAAGTTAAGGTCGAATTGAT+CGG 0.557387 8_3:-11707778 None:intergenic
GAAGAAAGAAACTATGAACT+AGG 0.564784 8_3:-11705456 None:intergenic
GAGACTCACCGGTAAGGCAC+CGG 0.568974 8_3:+11705585 Msa1276810:CDS
TTCTTGAGAAAAGAAAAGAA+AGG 0.582036 8_3:+11707565 Msa1276810:three_prime_UTR
GAATAGAATATCGTCAATTG+TGG 0.583205 8_3:-11707173 None:intergenic
GCCTTAGAAACAACCGAACA+AGG 0.584084 8_3:+11707361 Msa1276810:three_prime_UTR
AAATTTAGCTAATACTGTGT+CGG 0.588601 8_3:+11705555 Msa1276810:CDS
CTAGTGACCGGTGCACTTGG+GGG 0.600861 8_3:-11707298 None:intergenic
ATGATCTCAGTTGCATTCGT+AGG 0.602248 8_3:-11707227 None:intergenic
TGTTGCAGGGTCATTGCTAG+AGG 0.603165 8_3:+11707687 Msa1276810:intron
TTAGCTAATACTGTGTCGGA+TGG 0.619524 8_3:+11705559 Msa1276810:CDS
CAACATGCAAAACTCAGATG+GGG 0.638179 8_3:+11706031 Msa1276810:CDS
ATGCCCTAAGAAGATTGTTG+TGG 0.644601 8_3:+11707869 Msa1276810:three_prime_UTR
TTATGTAGATAAAGACCACA+TGG 0.687381 8_3:+11705371 Msa1276810:five_prime_UTR
AACATGCAAAACTCAGATGG+GGG 0.692839 8_3:+11706032 Msa1276810:CDS
GTCTGAACTATGAGACCATG+TGG 0.700545 8_3:-11705386 None:intergenic
TTAGCCTCTGAAAATCCAAG+CGG 0.722831 8_3:+11707266 Msa1276810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTAATTTTAATTTTTT+TGG + chr8_3:11705772-11705791 Msa1276810:intron 0.0%
!! AATAATAATAAAATTAAATT+AGG - chr8_3:11705843-11705862 None:intergenic 0.0%
!!! ATTATTATTTTGTTACAAAA+TGG + chr8_3:11705854-11705873 Msa1276810:intron 10.0%
!!! GATATTAGTATATTGTTTTT+TGG - chr8_3:11705293-11705312 None:intergenic 15.0%
!!! TTAATGTTTTATTTCTTACT+TGG + chr8_3:11705742-11705761 Msa1276810:intron 15.0%
!!! TTTAGTATTGGAAAATTATT+GGG + chr8_3:11705886-11705905 Msa1276810:intron 15.0%
!!! ATTTACATGAAAAAACATTT+TGG - chr8_3:11706245-11706264 None:intergenic 15.0%
!!! AATGTTTTTTCATGTAAATT+AGG + chr8_3:11706246-11706265 Msa1276810:intron 15.0%
!!! ATATTGGTTAGAATAATAAT+TGG + chr8_3:11706737-11706756 Msa1276810:intron 15.0%
!!! TGTAAATTAATTTTATTGTG+AGG - chr8_3:11706992-11707011 None:intergenic 15.0%
!!! TTTAGAGTATCATTTTATTT+TGG - chr8_3:11707016-11707035 None:intergenic 15.0%
!! AAATATATATTGTAAGAGTT+TGG + chr8_3:11707092-11707111 Msa1276810:intron 15.0%
!! TATTATGTTATCAATCAATT+AGG + chr8_3:11707938-11707957 Msa1276810:three_prime_UTR 15.0%
!!! GTAAAGTTAAGATTTTTTGT+TGG + chr8_3:11705511-11705530 Msa1276810:five_prime_UTR 20.0%
!!! GTTTAGTATTGGAAAATTAT+TGG + chr8_3:11705885-11705904 Msa1276810:intron 20.0%
!! TAGATCTATTTATAACAAAG+TGG + chr8_3:11706355-11706374 Msa1276810:intron 20.0%
!!! TAAATAAGTGTAGATTTGTT+TGG + chr8_3:11706666-11706685 Msa1276810:intron 20.0%
!! AAGGAAATAAAATAAACAGA+CGG - chr8_3:11707617-11707636 None:intergenic 20.0%
!! AACTAAATCACTATAAGTTA+AGG - chr8_3:11707794-11707813 None:intergenic 20.0%
!! TAAATCTATATGAAGATTGA+AGG - chr8_3:11707895-11707914 None:intergenic 20.0%
!! AATCACATGTATTCAAAAAA+TGG - chr8_3:11708082-11708101 None:intergenic 20.0%
!!! TTTTTTGAATACATGTGATT+TGG + chr8_3:11708082-11708101 Msa1276810:three_prime_UTR 20.0%
!!! AAGTTAAGATTTTTTGTTGG+TGG + chr8_3:11705514-11705533 Msa1276810:five_prime_UTR 25.0%
!!! AGTTAAGATTTTTTGTTGGT+GGG + chr8_3:11705515-11705534 Msa1276810:five_prime_UTR 25.0%
! AAATTTAGCTAATACTGTGT+CGG + chr8_3:11705555-11705574 Msa1276810:CDS 25.0%
! AAGAGAAAGATTATACCTTT+AGG - chr8_3:11705712-11705731 None:intergenic 25.0%
!! ATAATCTTTCTCTTGTTTCA+TGG + chr8_3:11705718-11705737 Msa1276810:intron 25.0%
! AACCAAACAATTAATTGACA+AGG - chr8_3:11705808-11705827 None:intergenic 25.0%
! TTGTTACAAAATGGATGATT+TGG + chr8_3:11705863-11705882 Msa1276810:intron 25.0%
!!! TGAAAAAACATTTTGGTAGT+AGG - chr8_3:11706238-11706257 None:intergenic 25.0%
!! ATGTAAATTAGGTTAGTCAT+GGG + chr8_3:11706257-11706276 Msa1276810:intron 25.0%
!!! GATACAATTCAGTTTTGAAA+AGG + chr8_3:11706292-11706311 Msa1276810:intron 25.0%
! ATCTTGATTAAAACAATCGT+CGG + chr8_3:11706436-11706455 Msa1276810:intron 25.0%
!! AACAGATCTATACTTCTTTT+TGG - chr8_3:11706504-11706523 None:intergenic 25.0%
!!! AATCTTTCATTTTGATCTTG+TGG - chr8_3:11706824-11706843 None:intergenic 25.0%
!! AGATTTGTTTGATTGAAAAG+AGG + chr8_3:11706839-11706858 Msa1276810:intron 25.0%
!! AGGTTGAAGTATTAATGATA+TGG - chr8_3:11706892-11706911 None:intergenic 25.0%
!! TACATGTTAGTGATCAATTT+TGG - chr8_3:11706921-11706940 None:intergenic 25.0%
! CTAACATGTATGTAAGAAAA+AGG + chr8_3:11706931-11706950 Msa1276810:intron 25.0%
! ATAAACAACAATAAGAACAC+TGG - chr8_3:11707123-11707142 None:intergenic 25.0%
!!! TTTAATTTTTCACTAGTGAC+CGG - chr8_3:11707313-11707332 None:intergenic 25.0%
! AACTAATTTACCTGAACATT+TGG - chr8_3:11707395-11707414 None:intergenic 25.0%
! TTCTTGAGAAAAGAAAAGAA+AGG + chr8_3:11707565-11707584 Msa1276810:three_prime_UTR 25.0%
!! CGGAAATAGAAATTTTTAAC+TGG - chr8_3:11707597-11707616 None:intergenic 25.0%
! CAAAAATAGACAACATACAA+AGG - chr8_3:11707636-11707655 None:intergenic 25.0%
!!! TGTATGTTGTCTATTTTTGT+TGG + chr8_3:11707637-11707656 Msa1276810:intron 25.0%
! CAAGATTCAATTAATTAGCA+TGG - chr8_3:11707664-11707683 None:intergenic 25.0%
!! TTGTGACCATTAATTTGATA+AGG - chr8_3:11707714-11707733 None:intergenic 25.0%
! AATTAATGGTCACAAAAATG+AGG + chr8_3:11707719-11707738 Msa1276810:three_prime_UTR 25.0%
! ATTAATGGTCACAAAAATGA+GGG + chr8_3:11707720-11707739 Msa1276810:three_prime_UTR 25.0%
!! TTTAGTTCATCTTGAACTTT+AGG + chr8_3:11707807-11707826 Msa1276810:three_prime_UTR 25.0%
!!! ACTTTAGGTTATACATGTTT+TGG + chr8_3:11707822-11707841 Msa1276810:three_prime_UTR 25.0%
TTATGTAGATAAAGACCACA+TGG + chr8_3:11705371-11705390 Msa1276810:five_prime_UTR 30.0%
GAAGAAAGAAACTATGAACT+AGG - chr8_3:11705459-11705478 None:intergenic 30.0%
!!! GTTAAGATTTTTTGTTGGTG+GGG + chr8_3:11705516-11705535 Msa1276810:five_prime_UTR 30.0%
TGTGAAAATTTGAACTTCTC+CGG - chr8_3:11705607-11705626 None:intergenic 30.0%
TTGAAGGAAAAGAGAATTTC+TGG + chr8_3:11705652-11705671 Msa1276810:CDS 30.0%
TGCCTTGTCAATTAATTGTT+TGG + chr8_3:11705803-11705822 Msa1276810:intron 30.0%
!! TGGATGATTTGGTTTAGTAT+TGG + chr8_3:11705874-11705893 Msa1276810:intron 30.0%
! ATTTTGCTGATCGTGTTTTT+TGG + chr8_3:11705970-11705989 Msa1276810:intron 30.0%
!! GATTTTTCTAGCAAGTACAA+AGG + chr8_3:11705998-11706017 Msa1276810:intron 30.0%
GAATTAGACTTCTTAATGCA+AGG - chr8_3:11706110-11706129 None:intergenic 30.0%
GCATTAAGAAGTCTAATTCA+AGG + chr8_3:11706111-11706130 Msa1276810:CDS 30.0%
! CATGTAAATTAGGTTAGTCA+TGG + chr8_3:11706256-11706275 Msa1276810:intron 30.0%
TTAGCAAAAGTCGAATTGTT+TGG + chr8_3:11706537-11706556 Msa1276810:intron 30.0%
TAGCAAAAGTCGAATTGTTT+GGG + chr8_3:11706538-11706557 Msa1276810:intron 30.0%
!! GTTTTGATCTTCTAGTCTAA+GGG - chr8_3:11706644-11706663 None:intergenic 30.0%
!!! GTTAGTGATCAATTTTGGTT+GGG - chr8_3:11706916-11706935 None:intergenic 30.0%
!! TGTTAGTGATCAATTTTGGT+TGG - chr8_3:11706917-11706936 None:intergenic 30.0%
! AGGTATTGTTGTGTTAAGTT+TGG + chr8_3:11706951-11706970 Msa1276810:intron 30.0%
GAATAGAATATCGTCAATTG+TGG - chr8_3:11707176-11707195 None:intergenic 30.0%
CAATTGACGATATTCTATTC+CGG + chr8_3:11707176-11707195 Msa1276810:CDS 30.0%
AATTGACGATATTCTATTCC+GGG + chr8_3:11707177-11707196 Msa1276810:CDS 30.0%
! GTCAAATACTAACATTTTCC+CGG - chr8_3:11707198-11707217 None:intergenic 30.0%
GGTCACTAGTGAAAAATTAA+AGG + chr8_3:11707312-11707331 Msa1276810:CDS 30.0%
AGTGTTTGTTTGAGGAATTT+CGG - chr8_3:11707341-11707360 None:intergenic 30.0%
TTGCATGCTTTGTAATGTTT+CGG - chr8_3:11707464-11707483 None:intergenic 30.0%
GAGAAAAGAAAAGAAAGGTA+TGG + chr8_3:11707570-11707589 Msa1276810:intron 30.0%
ATTGAATCTTGAATTGTTGC+AGG + chr8_3:11707673-11707692 Msa1276810:intron 30.0%
TTGAATCTTGAATTGTTGCA+GGG + chr8_3:11707674-11707693 Msa1276810:intron 30.0%
AGAGGACCTTATCAAATTAA+TGG + chr8_3:11707705-11707724 Msa1276810:three_prime_UTR 30.0%
TGATCGGAAGAATTATCTTT+AGG - chr8_3:11707765-11707784 None:intergenic 30.0%
TAAGTTAAGGTCGAATTGAT+CGG - chr8_3:11707781-11707800 None:intergenic 30.0%
!! ATCAGTTTTATCACTGAGAT+TGG + chr8_3:11707991-11708010 Msa1276810:three_prime_UTR 30.0%
!! TCTTTTGAGTCAGTTCTTGA+AGG + chr8_3:11705636-11705655 Msa1276810:CDS 35.0%
ATTCTACACCTGCTTTCTTT+GGG + chr8_3:11705677-11705696 Msa1276810:CDS 35.0%
TTTGGGAAAATGGAACCTAA+AGG + chr8_3:11705694-11705713 Msa1276810:CDS 35.0%
TCTAGACATCTAATGACAAG+TGG - chr8_3:11706339-11706358 None:intergenic 35.0%
TTATAACAAAGTGGCGCAAT+TGG + chr8_3:11706364-11706383 Msa1276810:intron 35.0%
! TAGCTTAGGTCGAAATGTTT+TGG + chr8_3:11706398-11706417 Msa1276810:intron 35.0%
TAAAATCGTGAATCAGTGAG+AGG + chr8_3:11706471-11706490 Msa1276810:intron 35.0%
CTTCTAGTCTAAGGGAAATT+GGG - chr8_3:11706636-11706655 None:intergenic 35.0%
TCTTCTAGTCTAAGGGAAAT+TGG - chr8_3:11706637-11706656 None:intergenic 35.0%
!! CGTTTTGATCTTCTAGTCTA+AGG - chr8_3:11706645-11706664 None:intergenic 35.0%
TTAGCTAAGGCCTAATTGTT+CGG + chr8_3:11706698-11706717 Msa1276810:intron 35.0%
GTAGTACTCGAGTTCAATAT+TGG + chr8_3:11706721-11706740 Msa1276810:intron 35.0%
TTTATTGTGAGGTTGAGATC+TGG - chr8_3:11706981-11707000 None:intergenic 35.0%
CTAAACTCGTTGAACATCAA+TGG + chr8_3:11707031-11707050 Msa1276810:intron 35.0%
! ATCGTCAATTGTGGAGTTTT+AGG - chr8_3:11707167-11707186 None:intergenic 35.0%
! ATTTTCAGAGGCTAATTTGG+TGG - chr8_3:11707261-11707280 None:intergenic 35.0%
! TGGATTTTCAGAGGCTAATT+TGG - chr8_3:11707264-11707283 None:intergenic 35.0%
GTGTTTGTTTGAGGAATTTC+GGG - chr8_3:11707340-11707359 None:intergenic 35.0%
ATTTACCTGAACATTTGGCT+TGG - chr8_3:11707390-11707409 None:intergenic 35.0%
TTAAGAAGTGAAGCTCTTCT+TGG - chr8_3:11707543-11707562 None:intergenic 35.0%
AAAAATGAGGGTAGTAGTTC+TGG + chr8_3:11707732-11707751 Msa1276810:three_prime_UTR 35.0%
AATCTTCTTAGGGCATAGTT+AGG - chr8_3:11707865-11707884 None:intergenic 35.0%
TCAATTAGGTCCAATGCTTT+CGG + chr8_3:11707952-11707971 Msa1276810:three_prime_UTR 35.0%
!!! TTGTTTTGATCCGAAAGCAT+TGG - chr8_3:11707965-11707984 None:intergenic 35.0%
CTTGTTCGTTGAATCTGGTT+TGG - chr8_3:11705411-11705430 None:intergenic 40.0%
! CTAAGCTTGTTCGTTGAATC+TGG - chr8_3:11705416-11705435 None:intergenic 40.0%
TTAGCTAATACTGTGTCGGA+TGG + chr8_3:11705559-11705578 Msa1276810:CDS 40.0%
GATTCTACACCTGCTTTCTT+TGG + chr8_3:11705676-11705695 Msa1276810:CDS 40.0%
! ACCTGCTTTCTTTGGGAAAA+TGG + chr8_3:11705684-11705703 Msa1276810:CDS 40.0%
! TCCATTTTCCCAAAGAAAGC+AGG - chr8_3:11705688-11705707 None:intergenic 40.0%
!!! CTGATCGTGTTTTTTGGTTG+TGG + chr8_3:11705976-11705995 Msa1276810:intron 40.0%
CCAACATGCAAAACTCAGAT+GGG + chr8_3:11706030-11706049 Msa1276810:CDS 40.0%
CAACATGCAAAACTCAGATG+GGG + chr8_3:11706031-11706050 Msa1276810:CDS 40.0%
AACATGCAAAACTCAGATGG+GGG + chr8_3:11706032-11706051 Msa1276810:CDS 40.0%
! AAATTAGGTTAGTCATGGGC+TGG + chr8_3:11706261-11706280 Msa1276810:intron 40.0%
AGACATCTAATGACAAGTGG+TGG - chr8_3:11706336-11706355 None:intergenic 40.0%
GTAGTACTTGAGTTCGATCT+TGG + chr8_3:11706560-11706579 Msa1276810:intron 40.0%
TACTTGAGTTCGATCTTGGT+TGG + chr8_3:11706564-11706583 Msa1276810:intron 40.0%
AGTCTAAGGGAAATTGGGTT+CGG - chr8_3:11706631-11706650 None:intergenic 40.0%
GAACAATTAGGCCTTAGCTA+AGG - chr8_3:11706699-11706718 None:intergenic 40.0%
TAGCTAAGGCCTAATTGTTC+GGG + chr8_3:11706699-11706718 Msa1276810:intron 40.0%
!!! GTGATCAATTTTGGTTGGGA+AGG - chr8_3:11706912-11706931 None:intergenic 40.0%
ACTCGTTGAACATCAATGGT+AGG + chr8_3:11707035-11707054 Msa1276810:intron 40.0%
ATGATCTCAGTTGCATTCGT+AGG - chr8_3:11707230-11707249 None:intergenic 40.0%
TACGAATGCAACTGAGATCA+TGG + chr8_3:11707229-11707248 Msa1276810:CDS 40.0%
! TTAGCCTCTGAAAATCCAAG+CGG + chr8_3:11707266-11707285 Msa1276810:CDS 40.0%
! TCTAAGGCAGTGTTTGTTTG+AGG - chr8_3:11707349-11707368 None:intergenic 40.0%
!! TCCTTGTTCGGTTGTTTCTA+AGG - chr8_3:11707365-11707384 None:intergenic 40.0%
!! TTTGGCTTGGTTTCCTTGTT+CGG - chr8_3:11707377-11707396 None:intergenic 40.0%
ATGCCCTAAGAAGATTGTTG+TGG + chr8_3:11707869-11707888 Msa1276810:three_prime_UTR 40.0%
AGGCCACAACAATCTTCTTA+GGG - chr8_3:11707875-11707894 None:intergenic 40.0%
AAGGCCACAACAATCTTCTT+AGG - chr8_3:11707876-11707895 None:intergenic 40.0%
GTCTGAACTATGAGACCATG+TGG - chr8_3:11705389-11705408 None:intergenic 45.0%
GCCAACATGCAAAACTCAGA+TGG + chr8_3:11706029-11706048 Msa1276810:CDS 45.0%
! CCCATCTGAGTTTTGCATGT+TGG - chr8_3:11706033-11706052 None:intergenic 45.0%
TGAATCTCTTCCTCAGCTTG+TGG + chr8_3:11706079-11706098 Msa1276810:CDS 45.0%
! CAAGGATTTTCCACAAGCTG+AGG - chr8_3:11706092-11706111 None:intergenic 45.0%
! TGGTTAATGCGCGTTAGCTT+AGG + chr8_3:11706384-11706403 Msa1276810:intron 45.0%
!! TTGGTTCTACGCCTTAGCTA+AGG + chr8_3:11706685-11706704 Msa1276810:intron 45.0%
CGAGTACTACCCGAACAATT+AGG - chr8_3:11706711-11706730 None:intergenic 45.0%
GTCGAGACTGAATTTCACTG+TGG + chr8_3:11706797-11706816 Msa1276810:intron 45.0%
TGCAACTGAGATCATGGAGT+TGG + chr8_3:11707235-11707254 Msa1276810:CDS 45.0%
! TTCACCGCTTGGATTTTCAG+AGG - chr8_3:11707273-11707292 None:intergenic 45.0%
GCCTTAGAAACAACCGAACA+AGG + chr8_3:11707361-11707380 Msa1276810:three_prime_UTR 45.0%
GGAAACCAAGCCAAATGTTC+AGG + chr8_3:11707382-11707401 Msa1276810:three_prime_UTR 45.0%
CTAATACTGTGTCGGATGGC+CGG + chr8_3:11705563-11705582 Msa1276810:CDS 50.0%
AAGAAAACCCCCAAGTGCAC+CGG + chr8_3:11707291-11707310 Msa1276810:CDS 50.0%
TGTTGCAGGGTCATTGCTAG+AGG + chr8_3:11707687-11707706 Msa1276810:intron 50.0%
GAACTTCTCCGGTGCCTTAC+CGG - chr8_3:11705596-11705615 None:intergenic 55.0%
! TGGGGGTTTTCTTCACCGCT+TGG - chr8_3:11707284-11707303 None:intergenic 55.0%
ACTAGTGACCGGTGCACTTG+GGG - chr8_3:11707302-11707321 None:intergenic 55.0%
CACTAGTGACCGGTGCACTT+GGG - chr8_3:11707303-11707322 None:intergenic 55.0%
TCACTAGTGACCGGTGCACT+TGG - chr8_3:11707304-11707323 None:intergenic 55.0%
GTGCCTTACCGGTGAGTCTC+CGG - chr8_3:11705585-11705604 None:intergenic 60.0%
GAGACTCACCGGTAAGGCAC+CGG + chr8_3:11705585-11705604 Msa1276810:CDS 60.0%
CTAGTGACCGGTGCACTTGG+GGG - chr8_3:11707301-11707320 None:intergenic 60.0%
TCGGATGGCCGGAGACTCAC+CGG + chr8_3:11705574-11705593 Msa1276810:CDS 65.0%
TGGCCGGAGACTCACCGGTA+AGG + chr8_3:11705579-11705598 Msa1276810:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 11705273 11708106 11705273 ID=Msa1276810;Name=Msa1276810
chr8_3 mRNA 11705273 11708106 11705273 ID=Msa1276810-mRNA-1;Parent=Msa1276810;Name=Msa1276810-mRNA-1;_AED=0.08;_eAED=0.08;_QI=277|1|1|1|0.75|0.8|5|542|155
chr8_3 exon 11705273 11705715 11705273 ID=Msa1276810-mRNA-1:exon:4719;Parent=Msa1276810-mRNA-1
chr8_3 exon 11706009 11706132 11706009 ID=Msa1276810-mRNA-1:exon:4720;Parent=Msa1276810-mRNA-1
chr8_3 exon 11707154 11707403 11707154 ID=Msa1276810-mRNA-1:exon:4721;Parent=Msa1276810-mRNA-1
chr8_3 exon 11707529 11707586 11707529 ID=Msa1276810-mRNA-1:exon:4722;Parent=Msa1276810-mRNA-1
chr8_3 exon 11707695 11708106 11707695 ID=Msa1276810-mRNA-1:exon:4723;Parent=Msa1276810-mRNA-1
chr8_3 five_prime_UTR 11705273 11705549 11705273 ID=Msa1276810-mRNA-1:five_prime_utr;Parent=Msa1276810-mRNA-1
chr8_3 CDS 11705550 11705715 11705550 ID=Msa1276810-mRNA-1:cds;Parent=Msa1276810-mRNA-1
chr8_3 CDS 11706009 11706132 11706009 ID=Msa1276810-mRNA-1:cds;Parent=Msa1276810-mRNA-1
chr8_3 CDS 11707154 11707331 11707154 ID=Msa1276810-mRNA-1:cds;Parent=Msa1276810-mRNA-1
chr8_3 three_prime_UTR 11707332 11707403 11707332 ID=Msa1276810-mRNA-1:three_prime_utr;Parent=Msa1276810-mRNA-1
chr8_3 three_prime_UTR 11707529 11707586 11707529 ID=Msa1276810-mRNA-1:three_prime_utr;Parent=Msa1276810-mRNA-1
chr8_3 three_prime_UTR 11707695 11708106 11707695 ID=Msa1276810-mRNA-1:three_prime_utr;Parent=Msa1276810-mRNA-1
Gene Sequence

>Msa1276810

ATGTCAAATTTAGCTAATACTGTGTCGGATGGCCGGAGACTCACCGGTAAGGCACCGGAGAAGTTCAAATTTTCACAGACTTGTAGTCTTTTGAGTCAGTTCTTGAAGGAAAAGAGAATTTCTGGTGATTCTACACCTGCTTTCTTTGGGAAAATGGAACCTAAAGCAAGTACAAAGGATTTGTTAGCCAACATGCAAAACTCAGATGGGGGTTTGAGACTAAATGCATCTGCTATTGAATCTCTTCCTCAGCTTGTGGAAAATCCTTGCATTAAGAAGTCTAATTCAAGATCAACAGATCCTAAAACTCCACAATTGACGATATTCTATTCCGGGAAAATGTTAGTATTTGACGCTTTTTCGCCTACGAATGCAACTGAGATCATGGAGTTGGCCACCAAATTAGCCTCTGAAAATCCAAGCGGTGAAGAAAACCCCCAAGTGCACCGGTCACTAGTGAAAAATTAA
Protein sequence

>Msa1276810

MSNLANTVSDGRRLTGKAPEKFKFSQTCSLLSQFLKEKRISGDSTPAFFGKMEPKASTKDLLANMQNSDGGLRLNASAIESLPQLVENPCIKKSNSRSTDPKTPQLTIFYSGKMLVFDAFSPTNATEIMELATKLASENPSGEENPQVHRSLVKN*